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Planktothrix Presentation29 07 3 3rd Webinar Planktothrix rubescens bbe - the company for advanced algae and cyanobacteria measurement www.bbe-moldaenke.de Welcome Detlev Lohse Frederike Lohse Sönke Kobarg bbe Team www.bbe-moldaenke.de Webinar – Planktotrix rubescens Part 1 Presentation 15 min Part 2 Demonstration 3 min Part 3 Interview 10 min Feedback Follow up www.bbe-moldaenke.de MONITORING THE APPEARANCE OF THE CYANOBACTERIA PLANKTOTHRIX RUBESCENS Detlev Lohse from the bbe Moldaenke company, Germany www.bbe-moldaenke.de Page 4 North Rhine-Westphalia 16,6 Mio. inhabitants (2013) Water consumption 130 l/day = 2,2 Mio. m 3/day total www.bbe-moldaenke.de Page 5 The area of interest in 2013 Sengbach dam 20 ha 2,8 Mio. m 3 Maximum depth 36 m www.bbe-moldaenke.de Page 6 Extraction for drinking water processing Planktothrix rubescens massive dam with waterworks Glüder www.bbe-moldaenke.de Page 7 The threat of the Planktothrix Reduce water quality Raise health risks Cyanotoxin Additional counteractions Raise costs Close of extraction Planktothrix rubescens www.bbe-moldaenke.de Page 8 WHO Guidelines 1999 www.bbe-moldaenke.de Page 9 How it works: fluorometric measurement of algal and cyanobacterial microplankton Excitation of pigments with light of different wavelengths Light emisson Accessory pigments + Chlorophyll a Photosynthetic membrane Determination of the red chlorophyll fluorescence www.bbe-moldaenke.de Page 10 What are the Algae classes? Algae classes contain different accessory pigments Spectral Green Blue Brown Red Mixed Group Peripheral Chlorophyll a/b- Phycobilisomes Chlorophyll a/c- Phycobilisomes Chlorophyll a/c- Antenna Xanthophyll (Phycocyanin) Xanthophyll (Phycoerythrin) Phycobiliprotein Heterokonto- Cyanophyta phyta Division Chlorophyta Rhodophyta Cryptophyta Glaucophyta Haptophyta Dinophyta …which affect the chlorophyll fluorescence emission in characteristic pattern www.bbe-moldaenke.de Page 11 Who is who in algae: norm spectra … represent characteristic red fluorescence of algal cells on excitation with different wavelengths www.bbe-moldaenke.de Page 12 Addition of Plantothrix rubescens spectrum www.bbe-moldaenke.de Page 13 Structure of phycobilisomes Phycoerythrin Phycocyanin Chlorophyll Allophycocyanin Thylakoid membrane www.bbe-moldaenke.de Page 14 Spectral features of the Planktothrix rubescens • phycobilins close the „gap“ in the absorption spectra of chlorophyll and carotenoids and act as antenna pigments • redish cyanobacteria contain phycoerythrin (Planktothrix rubescens ) www.bbe-moldaenke.de Page 15 What can multispectral fluorometry accomplish? Differentiation of algal classes in a complex mixture Determination of cyanobacteria i.e. Planktothrix rubescens Determination of chlorophyll content Correction for fluorescent interfering substances Field measurement www.bbe-moldaenke.de Page 16 Depth analysis: distribution of Planktothrix rubescens www.bbe-moldaenke.de Page 17 Gas vesicles • Protein cylinders • Hollow, gas filled • Water (cytoplama) exclusion • Sensitive to outer pressure • Sensitive to high photosynthesis rate www.bbe-moldaenke.de Page 18 Scheme of main types of active movements in cyanobacteria www.bbe-moldaenke.de Page 19 The point of extraction: compare the cell counts and the OnlineAnalyser www.bbe-moldaenke.de Page 20 FluoroProbe profiles in a German river dam E2 – E6 = raw water extraction points www.bbe-moldaenke.de Page 21 www.bbe-moldaenke.de Page 22 Conclusion Fluorometric depth profiling and flow through oneline analysis are a sensitive and rapid tool for the detection of upcoming Plankthotrix www.bbe-moldaenke.de Page 23 Acknowledgement Public Services Solingen Andreas Mokros, Waterworks Glüder www.bbe-moldaenke.de Page 24.
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  • Scholarworks@UNO
    University of New Orleans ScholarWorks@UNO University of New Orleans Theses and Dissertations Dissertations and Theses Summer 8-4-2011 Identification and characterization of enzymes involved in the biosynthesis of different phycobiliproteins in cyanobacteria Avijit Biswas University of New Orleans, [email protected] Follow this and additional works at: https://scholarworks.uno.edu/td Part of the Biochemistry, Biophysics, and Structural Biology Commons Recommended Citation Biswas, Avijit, "Identification and characterization of enzymes involved in the biosynthesis of different phycobiliproteins in cyanobacteria" (2011). University of New Orleans Theses and Dissertations. 446. https://scholarworks.uno.edu/td/446 This Dissertation-Restricted is protected by copyright and/or related rights. It has been brought to you by ScholarWorks@UNO with permission from the rights-holder(s). You are free to use this Dissertation-Restricted in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/or on the work itself. This Dissertation-Restricted has been accepted for inclusion in University of New Orleans Theses and Dissertations by an authorized administrator of ScholarWorks@UNO. For more information, please contact [email protected]. Identification and characterization of enzymes involved in biosynthesis of different phycobiliproteins in cyanobacteria A Thesis Submitted to the Graduate Faculty of the University of New Orleans in partial fulfillment of the requirements for the degree of Doctor of Philosophy In Chemistry (Biochemistry) By Avijit Biswas B.S.
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