Biology 6A Biological Form & Function Lab Manual
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The Glycosyltransferase Repertoire of the Spikemoss Selaginella Moellendorffiind a a Comparative Study of Its Cell Wall
Purdue University Purdue e-Pubs Department of Botany and Plant Pathology Faculty Department of Botany and Plant Pathology Publications 5-2-2012 The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffiind a a Comparative Study of Its Cell Wall. Jesper Harholt Iben Sørensen Cornell University Jonatan Fangel Alison Roberts University of Rhode Island William G.T. Willats See next page for additional authors Follow this and additional works at: http://docs.lib.purdue.edu/btnypubs Recommended Citation Harholt, Jesper; Sørensen, Iben; Fangel, Jonatan; Roberts, Alison; Willats, William G.T.; Scheller, Henrik Vibe; Petersen, Bent Larsen; Banks, Jo Ann; and Ulvskov, Peter, "The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffii and a Comparative Study of Its Cell Wall." (2012). Department of Botany and Plant Pathology Faculty Publications. Paper 15. http://dx.doi.org/10.1371/journal.pone.0035846 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact [email protected] for additional information. Authors Jesper Harholt, Iben Sørensen, Jonatan Fangel, Alison Roberts, William G.T. Willats, Henrik Vibe Scheller, Bent Larsen Petersen, Jo Ann Banks, and Peter Ulvskov This article is available at Purdue e-Pubs: http://docs.lib.purdue.edu/btnypubs/15 The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffii and a Comparative Study of Its Cell Wall Jesper Harholt1, Iben Sørensen1,2, Jonatan Fangel1, Alison Roberts3, William G. -
The Analysis of Pinus Pinaster Snrks Reveals Clues of the Evolution of This Family and a New Set of Abiotic Stress Resistance Biomarkers
agronomy Article The Analysis of Pinus pinaster SnRKs Reveals Clues of the Evolution of This Family and a New Set of Abiotic Stress Resistance Biomarkers Francisco Javier Colina * , María Carbó , Ana Álvarez , Luis Valledor * and María Jesús Cañal Plant Physiology, Department of Organisms and Systems Biology and University Institute of Biotechnology (IUBA), University of Oviedo, 33006 Oviedo, Spain; [email protected] (M.C.); [email protected] (A.Á.); [email protected] (M.J.C.) * Correspondence: [email protected] (F.J.C.); [email protected] (L.V.) Received: 15 January 2020; Accepted: 17 February 2020; Published: 19 February 2020 Abstract: Climate change is increasing the intensity and incidence of environmental stressors, reducing the biomass yields of forestry species as Pinus pinaster. Selection of new stress-tolerant varieties is thus required. Many genes related to plant stress signaling pathways have proven useful for this purpose with sucrose non-fermenting related kinases (SnRK), conserved across plant evolution and connected to different phosphorylation cascades within ABA- and Ca2+-mediated signaling pathways, as a good example. The modulation of SnRKs and/or the selection of specific SnRK alleles have proven successful strategies to increase plant stress resistance. Despite this, SnRKs have been barely studied in gymnosperms. In this work P. pinaster SnRK sequences (PpiSnRK) were identified through a homology- and domain-based sequence analysis using Arabidopsis SnRK sequences as query. Moreover, PpiSnRKs links to the gymnosperm stress response were modeled out of the known interactions of PpiSnRKs orthologs from other species with different signaling complexity. This approach successfully identified the pine SnRK family and predicted their central role into the gymnosperm stress response, linking them to ABA, Ca2+, sugar/energy and possibly ethylene signaling. -
Morphological Features of the Anther Development in Tomato Plants with Non-Specific Male Sterility
biology Article Morphological Features of the Anther Development in Tomato Plants with Non-Specific Male Sterility Inna A. Chaban 1, Neonila V. Kononenko 1 , Alexander A. Gulevich 1, Liliya R. Bogoutdinova 1, Marat R. Khaliluev 1,2,* and Ekaterina N. Baranova 1,* 1 All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia; [email protected] (I.A.C.); [email protected] (N.V.K.); [email protected] (A.A.G.); [email protected] (L.R.B.) 2 Moscow Timiryazev Agricultural Academy, Agronomy and Biotechnology Faculty, Russian State Agrarian University, Timiryazevskaya 49, 127550 Moscow, Russia * Correspondence: [email protected] (M.R.K.); [email protected] (E.N.B.) Received: 3 January 2020; Accepted: 12 February 2020; Published: 17 February 2020 Abstract: The study was devoted to morphological and cytoembryological analysis of disorders in the anther and pollen development of transgenic tomato plants with a normal and abnormal phenotype, which is characterized by the impaired development of generative organs. Various abnormalities in the structural organization of anthers and microspores were revealed. Such abnormalities in microspores lead to the blocking of asymmetric cell division and, accordingly, the male gametophyte formation. Some of the non-degenerated microspores accumulate a large number of storage inclusions, forming sterile mononuclear pseudo-pollen, which is similar in size and appearance to fertile pollen grain (looks like pollen grain). It was discussed that the growth of tapetal cells in abnormal anthers by increasing the size and ploidy level of nuclei contributes to this process. It has been shown that in transgenic plants with a normal phenotype, individual disturbances are also observed in the development of both male and female gametophytes. -
The Origin of Alternation of Generations in Land Plants
Theoriginof alternation of generations inlandplants: afocuson matrotrophy andhexose transport Linda K.E.Graham and LeeW .Wilcox Department of Botany,University of Wisconsin, 430Lincoln Drive, Madison,WI 53706, USA (lkgraham@facsta¡.wisc .edu ) Alifehistory involving alternation of two developmentally associated, multicellular generations (sporophyteand gametophyte) is anautapomorphy of embryophytes (bryophytes + vascularplants) . Microfossil dataindicate that Mid ^Late Ordovicianland plants possessed such alifecycle, and that the originof alternationof generationspreceded this date.Molecular phylogenetic data unambiguously relate charophyceangreen algae to the ancestryof monophyletic embryophytes, and identify bryophytes as early-divergentland plants. Comparison of reproduction in charophyceans and bryophytes suggests that the followingstages occurredduring evolutionary origin of embryophytic alternation of generations: (i) originof oogamy;(ii) retention ofeggsand zygotes on the parentalthallus; (iii) originof matrotrophy (regulatedtransfer ofnutritional and morphogenetic solutes fromparental cells tothe nextgeneration); (iv)origin of a multicellularsporophyte generation ;and(v) origin of non-£ agellate, walled spores. Oogamy,egg/zygoteretention andmatrotrophy characterize at least some moderncharophyceans, and arepostulated to represent pre-adaptativefeatures inherited byembryophytes from ancestral charophyceans.Matrotrophy is hypothesizedto have preceded originof the multicellularsporophytes of plants,and to represent acritical innovation.Molecular -
Microsporogenesis and Male Gametogenesis in Jatropha Curcas L. (Euphorbiaceae)1 Huanfang F
Journal of the Torrey Botanical Society 134(3), 2007, pp. 335–343 Microsporogenesis and male gametogenesis in Jatropha curcas L. (Euphorbiaceae)1 Huanfang F. Liu South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China, and Graduate School of Chinese Academy of Sciences, Beijing, 100039, China Bruce K. Kirchoff University of North Carolina at Greensboro, Department of Biology, 312 Eberhart, P.O. Box 26170, Greensboro, NC 27402-6170 Guojiang J. Wu and Jingping P. Liao2 South China Botanical Garden, Chinese Academy of Sciences, Key Laboratory of Digital Botanical Garden in Guangdong, Guangzhou, 510650, China LIU, H. F. (South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China, and Graduate School of Chinese Academy of Sciences, Beijing, 100039, China), B. K. KIRCHOFF (University of North Carolina at Greensboro, Department of Biology, 312 Eberhart, P.O. Box 26170, Greensboro, NC 27402-6170), G. J. WU, AND J. P. LIAO (South China Botanical Garden, Chinese Academy of Sciences, Key Laboratory of Digital Botanical Garden in Guangdong, Guangzhou, 510650, China). Microsporogenesis and male gametogenesis in Jatropha curcas L. (Euphorbiaceae). J. Torrey Bot. Soc. 134: 335–343. 2007.— Microsporogenesis and male gametogenesis of Jatropha curcas L. (Euphorbiaceae) was studied in order to provide additional data on this poorly studied family. Male flowers of J. curcas have ten stamens, which each bear four microsporangia. The development of the anther wall is of the dicotyledonous type, and is composed of an epidermis, endothecium, middle layer(s) and glandular tapetum. The cytokinesis following meiosis is simultaneous, producing tetrahedral tetrads. Mature pollen grains are two-celled at anthesis, with a spindle shaped generative cell. -
Spermatophyte Flora of Liangzi Lake Wetland Nature Reserve
E3S Web of Conferences 143, 02040 (2 020) https://doi.org/10.1051/e3sconf/20 2014302040 ARFEE 2019 Spermatophyte Flora of Liangzi Lake Wetland Nature Reserve Xinyang Zhang, Shijing He*, Rong Tao, and Huan Dai Wuhan Institute of Design and Sciences, Wuhan 430205, China Abstract. Based on route and sample-plot survey, plant resources of Liangzi Lake Wetland Nature Reserve were investigated. The result showed that there were 503 species of spermatophyte belonging to 296 genera of 86 families. There were 5 species under national first and second level protection. The dominant families of spermatophyte contained 20 species and above. The dominant genera of spermatophyte contained 4 species and below. The 86 families of spermatophyte can be divided into 7 distribution types and 4 variants. Tropic distribution type was dominant, accounting for 70.83% in total (excluding cosmopolitans). The 296 genera of spermatophyte can be divided into 14 distribution types and 9 variants. Temperate elements were a little more than tropical elements, accounting for 50.84% and 49.16% in total (excluding cosmopolitans) respectively. Reserve had 3 Chinese endemic genera, reflecting certain ancient and relict. The purpose of the research is to provide background information and scientific basis for the protection, construction, management and rational utilization of plant resources in the reserve. 1 Preface temperature is 17℃, the annual average rainfall is 1663mm, the average sunshine hour is 2061 hours, and Flora refers to the sum of all plant species in a certain the frost free period is 270 days. The rain bearing area is region or country. It is the result of the development and 208500 hectares, and the annual average water level is evolution of the plant kingdom under certain natural and 17.81m. -
Curriculum Vitae
CURRICULUM VITAE ORCID ID: 0000-0003-0186-6546 Gar W. Rothwell Edwin and Ruth Kennedy Distinguished Professor Emeritus Department of Environmental and Plant Biology Porter Hall 401E T: 740 593 1129 Ohio University F: 740 593 1130 Athens, OH 45701 E: [email protected] also Courtesy Professor Department of Botany and PlantPathology Oregon State University T: 541 737- 5252 Corvallis, OR 97331 E: [email protected] Education Ph.D.,1973 University of Alberta (Botany) M.S., 1969 University of Illinois, Chicago (Biology) B.A., 1966 Central Washington University (Biology) Academic Awards and Honors 2018 International Organisation of Palaeobotany lifetime Honorary Membership 2014 Fellow of the Paleontological Society 2009 Distinguished Fellow of the Botanical Society of America 2004 Ohio University Distinguished Professor 2002 Michael A. Cichan Award, Botanical Society of America 1999-2004 Ohio University Presidential Research Scholar in Biomedical and Life Sciences 1993 Edgar T. Wherry Award, Botanical Society of America 1991-1992 Outstanding Graduate Faculty Award, Ohio University 1982-1983 Chairman, Paleobotanical Section, Botanical Society of America 1972-1973 University of Alberta Dissertation Fellow 1971 Paleobotanical (Isabel Cookson) Award, Botanical Society of America Positions Held 2011-present Courtesy Professor of Botany and Plant Pathology, Oregon State University 2008-2009 Visiting Senior Researcher, University of Alberta 2004-present Edwin and Ruth Kennedy Distinguished Professor of Environmental and Plant Biology, Ohio -
External Morphological Characteristics for Periods During Pecan Microspore and Pollen Differentiation I.E
J. AMER. Soc. HORT. SCI. 117(1):190-196. 1992. External Morphological Characteristics for Periods during Pecan Microspore and Pollen Differentiation I.E. Yates Russell Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA 30613 Darrell Sparks Department of Horticulture, University of Georgia, Athens, GA 30602 Additional index words. Carya illinoensis, staminate flower, meiosis, cytology, cytochemistry, electron microscopy Abstract. Catkin external morphological characteristics of a protogynous (’Stuart’) and a protandrous (’Desirable’) cultivar of pecan [Carya illinoensis (Wangenh.) C. Koch] were related temporally to the differentiation of microspore and pollen grains. Reproductive cell development was divided into seven periods based on evaluations of number, location, and intensity of staining of the nucleus and/or nucleolus; and vacuolization and staining intensity of the cytoplasm. Catkins with anthers and bracteoles enclosed by bracts did not have reproductive cells that were matured to free microspore. Free microspore developed only after bracteoles became externally visible. The Period 1 nucleus was at the periphery of the cell and a large central vacuole was present; at Period 2, the nucleus was at the center and vacuolation had been reduced. As the angle between the bract and catkin rachis increased to 45°, vacnolation was reduced as the nucleus enlarged and moved to a central location in the microspore (Periods 3 and 4). The majority of the pollen grains were binucleate, and the generative nucleus became elliptical (Periods 5 and 6) by the time anthers became externally visible. Acetocarmine staining intensity of cellular components masked the presence of the gener- ative nucleus (Period 7) just before anther dehiscence. -
Evolution of the Life Cycle in Land Plants
Journal of Systematics and Evolution 50 (3): 171–194 (2012) doi: 10.1111/j.1759-6831.2012.00188.x Review Evolution of the life cycle in land plants ∗ 1Yin-Long QIU 1Alexander B. TAYLOR 2Hilary A. McMANUS 1(Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA) 2(Department of Biological Sciences, Le Moyne College, Syracuse, NY 13214, USA) Abstract All sexually reproducing eukaryotes have a life cycle consisting of a haploid and a diploid phase, marked by meiosis and syngamy (fertilization). Each phase is adapted to certain environmental conditions. In land plants, the recently reconstructed phylogeny indicates that the life cycle has evolved from a condition with a dominant free-living haploid gametophyte to one with a dominant free-living diploid sporophyte. The latter condition allows plants to produce more genotypic diversity by harnessing the diversity-generating power of meiosis and fertilization, and is selectively favored as more solar energy is fixed and fed into the biosystem on earth and the environment becomes more heterogeneous entropically. Liverworts occupy an important position for understanding the origin of the diploid generation in the life cycle of land plants. Hornworts and lycophytes represent critical extant transitional groups in the change from the gametophyte to the sporophyte as the independent free-living generation. Seed plants, with the most elaborate sporophyte and the most reduced gametophyte (except the megagametophyte in many gymnosperms), have the best developed sexual reproduction system that can be matched only by mammals among eukaryotes: an ancient and stable sex determination mechanism (heterospory) that enhances outcrossing, a highly bimodal and skewed distribution of sperm and egg numbers, a male-driven mutation system, female specialization in mutation selection and nourishment of the offspring, and well developed internal fertilization. -
Diversity and Evolution of the Megaphyll in Euphyllophytes
G Model PALEVO-665; No. of Pages 16 ARTICLE IN PRESS C. R. Palevol xxx (2012) xxx–xxx Contents lists available at SciVerse ScienceDirect Comptes Rendus Palevol w ww.sciencedirect.com General palaeontology, systematics and evolution (Palaeobotany) Diversity and evolution of the megaphyll in Euphyllophytes: Phylogenetic hypotheses and the problem of foliar organ definition Diversité et évolution de la mégaphylle chez les Euphyllophytes : hypothèses phylogénétiques et le problème de la définition de l’organe foliaire ∗ Adèle Corvez , Véronique Barriel , Jean-Yves Dubuisson UMR 7207 CNRS-MNHN-UPMC, centre de recherches en paléobiodiversité et paléoenvironnements, 57, rue Cuvier, CP 48, 75005 Paris, France a r t i c l e i n f o a b s t r a c t Article history: Recent paleobotanical studies suggest that megaphylls evolved several times in land plant st Received 1 February 2012 evolution, implying that behind the single word “megaphyll” are hidden very differ- Accepted after revision 23 May 2012 ent notions and concepts. We therefore review current knowledge about diverse foliar Available online xxx organs and related characters observed in fossil and living plants, using one phylogenetic hypothesis to infer their origins and evolution. Four foliar organs and one lateral axis are Presented by Philippe Taquet described in detail and differ by the different combination of four main characters: lateral organ symmetry, abdaxity, planation and webbing. Phylogenetic analyses show that the Keywords: “true” megaphyll appeared at least twice in Euphyllophytes, and that the history of the Euphyllophytes Megaphyll four main characters is different in each case. The current definition of the megaphyll is questioned; we propose a clear and accurate terminology in order to remove ambiguities Bilateral symmetry Abdaxity of the current vocabulary. -
Late Devonian Spermatophyte Diversity and Paleoecology at Red Hill, North-Central Pennsylvania, U.S.A. Walter L
West Chester University Digital Commons @ West Chester University Geology & Astronomy Faculty Publications Geology & Astronomy 2010 Late Devonian spermatophyte diversity and paleoecology at Red Hill, north-central Pennsylvania, U.S.A. Walter L. Cressler III West Chester University, [email protected] Cyrille Prestianni Ben A. LePage Follow this and additional works at: http://digitalcommons.wcupa.edu/geol_facpub Part of the Geology Commons, Paleobiology Commons, and the Paleontology Commons Recommended Citation Cressler III, W.L., Prestianni, C., and LePage, B.A. 2010. Late Devonian spermatophyte diversity and paleoecology at Red Hill, north- central Pennsylvania, U.S.A. International Journal of Coal Geology 83, 91-102. This Article is brought to you for free and open access by the Geology & Astronomy at Digital Commons @ West Chester University. It has been accepted for inclusion in Geology & Astronomy Faculty Publications by an authorized administrator of Digital Commons @ West Chester University. For more information, please contact [email protected]. ARTICLE IN PRESS COGEL-01655; No of Pages 12 International Journal of Coal Geology xxx (2009) xxx–xxx Contents lists available at ScienceDirect International Journal of Coal Geology journal homepage: www.elsevier.com/locate/ijcoalgeo Late Devonian spermatophyte diversity and paleoecology at Red Hill, north-central Pennsylvania, USA Walter L. Cressler III a,⁎, Cyrille Prestianni b, Ben A. LePage c a Francis Harvey Green Library, 29 West Rosedale Avenue, West Chester University, West Chester, PA, 19383, USA b Université de Liège, Boulevard du Rectorat B18, Liège 4000 Belgium c The Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA, 19103 and PECO Energy Company, 2301 Market Avenue, S9-1, Philadelphia, PA 19103, USA article info abstract Article history: Early spermatophytes have been discovered at Red Hill, a Late Devonian (Famennian) fossil locality in north- Received 9 January 2009 central Pennsylvania, USA. -
Structure and Function of Spores in the Aquatic Heterosporous Fern Family Marsileaceae
Int. J. Plant Sci. 163(4):485–505. 2002. ᭧ 2002 by The University of Chicago. All rights reserved. 1058-5893/2002/16304-0001$15.00 STRUCTURE AND FUNCTION OF SPORES IN THE AQUATIC HETEROSPOROUS FERN FAMILY MARSILEACEAE Harald Schneider1 and Kathleen M. Pryer2 Department of Botany, Field Museum of Natural History, 1400 South Lake Shore Drive, Chicago, Illinois 60605-2496, U.S.A. Spores of the aquatic heterosporous fern family Marsileaceae differ markedly from spores of Salviniaceae, the only other family of heterosporous ferns and sister group to Marsileaceae, and from spores of all ho- mosporous ferns. The marsileaceous outer spore wall (perine) is modified above the aperture into a structure, the acrolamella, and the perine and acrolamella are further modified into a remarkable gelatinous layer that envelops the spore. Observations with light and scanning electron microscopy indicate that the three living marsileaceous fern genera (Marsilea, Pilularia, and Regnellidium) each have distinctive spores, particularly with regard to the perine and acrolamella. Several spore characters support a division of Marsilea into two groups. Spore character evolution is discussed in the context of developmental and possible functional aspects. The gelatinous perine layer acts as a flexible, floating organ that envelops the spores only for a short time and appears to be an adaptation of marsileaceous ferns to amphibious habitats. The gelatinous nature of the perine layer is likely the result of acidic polysaccharide components in the spore wall that have hydrogel (swelling and shrinking) properties. Megaspores floating at the water/air interface form a concave meniscus, at the center of which is the gelatinous acrolamella that encloses a “sperm lake.” This meniscus creates a vortex-like effect that serves as a trap for free-swimming sperm cells, propelling them into the sperm lake.