Chocolate, Theobromine, Dogs, and Other Great Stuff
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Cocoa Bean Shell—A By-Product with Nutritional Properties and Biofunctional Potential
nutrients Review Cocoa Bean Shell—A By-Product with Nutritional Properties and Biofunctional Potential Olga Rojo-Poveda 1,2,* , Letricia Barbosa-Pereira 2,3 , Giuseppe Zeppa 2,* and Caroline Stévigny 1,* 1 RD3 Department-Unit of Pharmacognosy, Bioanalysis and Drug Discovery, Faculty of Pharmacy, Université libre de Bruxelles, 1050 Brussels, Belgium 2 Department of Agriculture, Forestry and Food Sciences (DISAFA), University of Turin, 10095 Grugliasco, Italy 3 Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain; [email protected] * Correspondence: [email protected] (O.R.-P.); [email protected] (G.Z.); [email protected] (C.S.) Received: 20 March 2020; Accepted: 15 April 2020; Published: 17 April 2020 Abstract: Cocoa bean shells (CBS) are one of the main by-products from the transformation of cocoa beans, representing 10%-17% of the total cocoa bean weight. Hence, their disposal could lead to environmental and economic issues. As CBS could be a source of nutrients and interesting compounds, such as fiber (around 50% w/w), cocoa volatile compounds, proteins, minerals, vitamins, and a large spectrum of polyphenols, CBS may be a valuable ingredient/additive for innovative and functional foods. In fact, the valorization of food by-products within the frame of a circular economy is becoming crucial due to economic and environmental reasons. The aim of this review is to look over the chemical and nutritional composition of CBS and to revise the several uses that have been proposed in order to valorize this by-product for food, livestock feed, or industrial usages, but also for different medical applications. -
Recommended International Code of Practice
CAC/RCP 72-2013 Page 1 of 9 CODE OF PRACTICE FOR THE PREVENTION AND REDUCTION OF OCHRATOXIN A CONTAMINATION IN COCOA (CAC/RCP 72-2013) 1. INTRODUCTION 1. This document is intended to provide guidance for all interested parties producing and handling cocoa beans for human consumption. All cocoa beans should be prepared and handled in accordance with the General Principles of Food Hygiene1, which are relevant for all foods being prepared for human consumption. This code of practice indicates the measures that should be implemented by all persons that have the responsibility for assuring that food is safe and suitable for consumption. 2. Ochratoxin A (OTA) is a toxic fungal metabolite classified by the International Agency for Research on Cancer as a possible human carcinogen (group 2B). JECFA established a PTWI of 100 ng/kg bodyweight for OTA. OTA is produced by a few species in the genera Aspergillus and Penicillium. In cocoa beans, the studies have shown that only Aspergillus species, specifically A. carbonarius and A. niger agregate, with lower numbers of A. westerdijkiae, A. ochraceus and A. melleus are involved. OTA is produced when favourable conditions of water activity, nutrition and temperature required for growth of fungi and OTA biosynthesis are present. 3. The fruit of cocoa derived from the cocoa tree, Theobroma cacao L., is composed of pericarp, tissue that arises from the ripened ovary wall of a fruit, and the ovary. When the fruit is ripe the external tissue, also known as the pod, consisting of thick and hard organic material, could be used as compost, animal feed and a source of potash. -
The Xanthines (Theobromine and Aminophyllin)
effect of this might conceivably escape detection in THE XANTHINES (THEOBROMINE AND individuals engaged in very heavy work. From this AMINOPHYLLINE) IN THE TREAT- point of view our subjects were particularly favorable for this since of as MENT OF CARDIAC PAIN study, most them, shown in the table, were not engaged in any occupation. HARRY M.D. GOLD, GLYCERYL TRINITRATE TEST NATHANIEL T. M.D. KWIT, Early in the course of the study it was believed AND desirable to HAROLD M.D. restrict the selection of patients to those OTTO, who could establish their qualifications for service in NEW YORK such a study as this by their ability to distinguish An endeavor was made in this study to secure evi- between the efficacy of glyceryl trinitrate taken under dence on the question of whether the xanthines relieve the tongue and a soluble placebo tablet taken in the cardiac pain. same manner for relief during attacks of pain. The SELECTION OF PATIENTS discovery of several patients who found the two equally effective those who had suffered an of The were 100 ambulant in attend- among attack subjects patients thrombosis and were to thoracic ance at the cardiac in whom the of coronary subject pain clinic, diagnosis on effort led us to abandon this restriction. arteriosclerotic heart disease with cardiac pain was made, in accordance with the nomenclature and criteria The results obtained in sixty patients in whom the the New York Heart Association.1 glyceryl trinitrate test was made are of some interest. adopted by These received trinitrate were selected from a total case load of patients glyceryl tablets, %0o They approxi- or cr 0.4 which were mately 700 patients, representing an average sample /4so grain (0.6 mg.), they of the cardiac clinic several racial directed to take under the tongue at the onset of an population, comprising attack of In of these of groups, both native and born. -
The Dark, the Milk, the White Chocolate Recipes from Around the World Pdf, Epub, Ebook
CHOCOLATE IS THE NEW SEXY : THE DARK, THE MILK, THE WHITE CHOCOLATE RECIPES FROM AROUND THE WORLD PDF, EPUB, EBOOK Clydex | 54 pages | 24 Nov 2014 | Createspace Independent Publishing Platform | 9781976580192 | English | none Chocolate is The New Sexy : The Dark, The Milk, The White Chocolate recipes from around the world PDF Book I could find nothing to dispute what I had written on semi-sweet chocolate. Ever since she began contributing to the site several years ago, Mary has embraced the exciting challenge of being a wiseGEEK researcher and writer. Cocoa powder is made when the cacao liquor is pressed to remove the cocoa butter, resulting in a fine, unsweetened powder. They even say it has some health benefits, so you don't have to feel bad when you indulge. If it feels gritty or rough, you're feeling the sugar crystals and it's moisture bloom. All products linked here have been independently selected by our editors. For bakers, chocolate is one hell of a complicated ingredient—over volatile compounds contribute to its aroma and flavor. Rhonda Ward December 20, Tony Buys would do you share your recipes? Milk Chocolate : All of the above, plus milk solids. Its bitterness comes from pure nibs, the finely ground centers of roasted cocoa beans. Name required. Sugar and vanilla are also added to make this chocolate creamy and to enhance the flavor. Season to taste with additional salt or vanilla, and serve hot. Also called "drinking chocolate," many of the most luscious recipes melt real chocolate into warm milk, creating the ultimate comfort drinks. -
Effect of Practical Timing of Dosage on Theophylline Blood Levels In
Arch Dis Child: first published as 10.1136/adc.53.2.167 on 1 February 1978. Downloaded from Archives of Disease in Childhood, 1978, 53, 167-182 Short reports Effect of practical timing of dosage Methods on theophylline blood levels in For the period of the study, the children did not asthmatic children treated with receive tea, which contains theophylline. choline theophyllinate GroupA. These childrenwere given choline theophyll- The bronchodilator effect of theophylline has been inate in a dosage of as near as possible 8 mg/kg per known for over 50 years, but although it has been dose qds (mean 7 9 mg/kg, range 7-2-8 7 mg/kg), widely used both intravenously and rectally in the starting with half the dose on the first day to try and treatment ofacute asthma, its use as an oral broncho- minimise nausea. The doses were given at 0800 h, dilator has been limited by inadequate knowledge of 1300 h, 1700 h, and 2100 h, times considered suitable the correct dosage of the oral preparations. The for administration outside hospital. On the third day therapeutic level of theophylline is generally con- ofthe study serum levels were measured 2 hours after sidered to be between 10 and 20 ,ug/ml (Turner- each dose and before the 0800 h dose the following Warwick, 1957; Jenne et al., 1972), although sub- morning. optimal bronchodilation can be achieved with levels of 5 ,ug/ml or less (Maselli et al., 1970; Nicholson and Group B. These children were given cholinetheophyll- Chick, 1973). Serious toxicity with levels of less than inate in a dosage of as near as possible 10 mg/kg per copyright. -
UHPLC-HRMS Analysis of Theobromine in Theobroma Cacao
ition & F tr oo u d N f S o c Mladenovic et al., J Nutr Food Sci 2018, 8:6 l i e a n n r c DOI: 10.4172/2155-9600.1000737 e u s o J Journal of Nutrition & Food Sciences ISSN: 2155-9600 Research Article Open Access UHPLC-HRMS Analysis of Theobromine in Theobroma cacao and its Products Katarina Mladenovic, Yuriko Root and Dilrukshi Ramanathan* New Jersey Center for Science, Technology and Mathematics, Kean University, USA Abstract Theobroma cacao seed is the major ingredient in all types of chocolate products and contains Theobromine. Theobromine is a bitter alkaloid beneficial in the treatment of hypertension, arteriosclerosis and angina pectoris. Of all chocolate brand samples, Sample J containing 70% Cacao had the highest amount of theobromine. Sample A containing 11% Cacao had the least amount of theobromine. The 100% Cacao Chocolate Bar had the highest concentration of theobromine in comparison to roasted cocoa having the lowest concentration of theobromine. Quantitative analysis of Theobromine was completed on the Thermo Scientific UHPLC and LTQ Orbit rap Discovery equipped with an ESI ion source. A three-minute gradient method with a flow rate of 300 μL/min was developed on the UHPLC-HRMS using HPLC-grade water and acetonitrile. Ethyl ether was used to remove cacao fats and water was used to isolate theobromine. To obtain the precision of the theobromine extraction process, the recovery analysis was 86%. Keywords: Theobromine cacao; Hypertension; Smooth muscle; Frosty Pod Rot (Moniliasis) devastated Theobroma cacao species in Psychological effects; Carbohydrates Central/South America by 80% [9]. -
Pharmacological Research Theobroma Cacao L., the Food Of
Pharmacological Research 61 (2010) 5–13 Contents lists available at ScienceDirect Pharmacological Research journal homepage: www.elsevier.com/locate/yphrs Review Theobroma cacao L., the Food of the Gods: A scientific approach beyond myths and claims M. Rusconi ∗, A. Conti Alpine Foundation for Life Sciences (AFLS), 6718 Olivone, Switzerland article info abstract Article history: Cocoa beans are rich source of polyphenols, contributing about 10% of the dry weight of the whole bean Received 2 June 2009 and its derivative chocolate, particularly dark chocolate, is considered one of the major contributors of Received in revised form 30 August 2009 antioxidants to the American diet after fruits and vegetables. At present the wide variation in cocoa Accepted 31 August 2009 processing and in the content and profile of polyphenols make it difficult to determine to what extent the findings about positive effects expressed in different studies, translate into tangible clinical benefits. Keywords: Moreover, before claiming any healthy properties to a plant, natural product or food item on human Theobroma cacao L. subject, a basic research project approved by scientific and ethical commissions has to be performed. Chocolate Polyphenols Until now the definition, composition, manufacturing specifications, packaging and labelling of cocoa Health claims and chocolate products in Europe, are regulated by “Directive 2000/36/EC of the European parliament and of the council”. The definitions take changes in consumer tastes, chocolate composition and labelling into account, but do not consider the real potential of healthy, beneficial and nutraceutical effects. In fact, they fail to establish an official analytical methodology for the quantification of phenolic compounds in cocoa and chocolate. -
3-Methylxanthine Production Through Biodegradation of Theobromine By
Zhou et al. BMC Microbiology (2020) 20:269 https://doi.org/10.1186/s12866-020-01951-z RESEARCH ARTICLE Open Access 3-Methylxanthine production through biodegradation of theobromine by Aspergillus sydowii PT-2 Binxing Zhou1*† , Cunqiang Ma1,2,3*†, Chengqin Zheng1, Tao Xia4, Bingsong Ma1 and Xiaohui Liu1 Abstract Background: Methylxanthines, including caffeine, theobromine and theophylline, are natural and synthetic compounds in tea, which could be metabolized by certain kinds of bacteria and fungi. Previous studies confirmed that several microbial isolates from Pu-erh tea could degrade and convert caffeine and theophylline. We speculated that these candidate isolates also could degrade and convert theobromine through N-demethylation and oxidation. In this study, seven tea-derived fungal strains were inoculated into various theobromine agar medias and theobromine liquid mediums to assess their capacity in theobromine utilization. Related metabolites with theobromine degradation were detected by using HPLC in the liquid culture to investigate their potential application in the production of 3-methylxanthine. Results: Based on theobromine utilization capacity, Aspergillus niger PT-1, Aspergillus sydowii PT-2, Aspergillus ustus PT-6 and Aspergillus tamarii PT-7 have demonstrated the potential for theobromine biodegradation. Particularly, A. sydowii PT-2 and A. tamarii PT-7 could degrade theobromine significantly (p < 0.05) in all given liquid mediums. 3,7- Dimethyluric acid, 3-methylxanthine, 7-methylxanthine, 3-methyluric acid, xanthine, and uric acid were detected in A. sydowii PT-2 and A. tamarii PT-7 culture, respectively, which confirmed the existence of N-demethylation and oxidation in theobromine catabolism. 3-Methylxanthine was common and main demethylated metabolite of theobromine in the liquid culture. -
Exploring Cocoa Properties: Is Theobromine a Cognitive Modulator?
Psychopharmacology https://doi.org/10.1007/s00213-019-5172-0 REVIEW Exploring cocoa properties: is theobromine a cognitive modulator? Ilaria Cova1 & V. Leta1,2 & C. Mariani2 & L. Pantoni2 & S. Pomati1 Received: 7 May 2018 /Accepted: 16 January 2019 # Springer-Verlag GmbH Germany, part of Springer Nature 2019 Abstract Nutritional qualities of cocoa have been acknowledged by several authors; a particular focus has been placed on its high content of flavanols, known for their excellent antioxidant properties and subsequent protective effect on cardio- and cerebrovascular systems as well as for neuromodulatory and neuroprotective actions. Other active components of cocoa are methylxanthines (caffeine and theobromine). Whereas the effects of caffeine are extensively researched, the same is not the case for theobromine; this review summarizes evidence on the effect of theobromine on cognitive functions. Considering animal studies, it can be asserted that acute exposition to theobromine has a reduced and delayed nootropic effect with respect to caffeine, whereas both animal and human studies suggested a potential neuroprotective action of long-term assumption of theobromine through a reduction of Aβ amyloid pathology, which is commonly observed in Alzheimer’s disease patients’ brains. Hence, the conceiv- able action of theobromine alone and associated with caffeine or other cocoa constituents on cognitive modulation is yet underexplored and future studies are needed to shed light on this promising molecule. Keywords Cocoa . Theobromine . Cognitive modulator . Cognition Introduction predominant fraction of triglyceride molecules species (in particular oleic, stearic, palmitic, and linoleic acid) Theobroma cacao and its products (Pittenauer and Allmaier 2009); proteins contribute to 10–15% of the dry weight of cocoa seeds and consist Cocoa comes from the processing of seeds of a tropical tree, mainly of albumin and globulin fractions (Zak and considered by the Aztecs as a sacred plant. -
2 Cocoa Plants Purpose • Students Know the History of the Cocoa Plant
2 Cocoa Plants Purpose • Students know the history of the cocoa plant. • Students can name botanical characteristics of cocoa plants. • Students can name factors which are important for the growth of cocoa plants and describe diseases and pests of the plant. Overview • Basic historical, botanical and ecological knowledge about the cocoa plant. Time • 90-135 minutes Material • Printed pictures of the history of the cocoa plant • Cocoa pod • Cocoa tree (or pictures of a cocoa tree) • Handouts for each student Teachers Support Procedure: O) Preparation Order (Homework Task) • Give the students this worksheet a week in advance as a preparatory homework assignment. Students interview someone they know and that has cocoa farm about growth factors and diseases of cocoa trees. The results are used in C) Ecology and cocoa cultivation. ( Through the interview, the students establish a connection between the topic of the teaching unit and the world in which they live. In addition, the interview provides the opportunity to acquire specialist knowledge.) A) History of cocoa tree • Show a cocoa pod (or a picture of a cocoa tree) and ask the students where the cocoa plant has its origin (e.g. individual students answer / individual students show their guess on a map / the teacher names continents and the students raise their hand at their guess). • Form groups and distribute pictures of the history of the cocoa tree to groups. • Draw a timeline on the blackboard (4000 BC – today). Each group briefly discusses what their picture might represent, what it might have to do with the history of the cocoa tree and what time it alludes to. -
Lonely Planet's Global Chocolate Tour 1 Preview
CONTENTS Introduction 3 Costa Rica 60 Europe 146 The Beans 4 Cuba 64 Austria 148 Cacao to Chocolate 6 Ecuador 66 Belgium 152 INTRODUCTION Types of Chocolate 8 Honduras 70 Eastern Europe 160 From camel milk chocolate in Dubai to honeycomb We couldn't cover every worthy Swiss chocolatier or Glossary 11 Mexico 72 France 164 chocolate in Australia, single-origin chocolate ice incredible Parisian chocolate boutique, but we included Nicaragua 80 Germany 176 cream in San Francisco and chocolate-covered blueberries favourites from Lonely Planet writers across the world. The Africa & The Middle East 12 Peru 82 Iceland 184 from Trappist Monks in Quebec, the world of chocolate has major cacao-growing countries are represented as well, Cote d’Ivoire 14 Chocomuseos 84 Ireland 186 never been more diverse...or more delectable. Innovative often with tours of cacao farms where it's possible to see Ghana 16 USA 86 Italy 188 chocolatiers are thinking up novel ingredient combinations the crop as it's grown and harvested. While most production Israel & Palestinian Territories 18 Top Chocolate Festivals 116 Netherlands 194 from Ho Chi Minh City to Texas and finding new means of of chocolate is done elsewhere and growers in places like São Tomé & Príncipe 22 Portugal 200 sourcing from and supporting small cacao farmers in the Côte d'Ivoire and Costa Rica primarily export the raw crop South Africa 24 Asia 118 Spain 202 race to elevate each bite into chocolate heaven. Yet not without much in-country chocolate production of their own, United Arab Emirates 30 India 120 Switzerland 206 every chocolate destination in this book is a craft bean-to- new bar-makers are popping up all over to challenge the Top Hot Chocolates 32 Indonesia 122 United Kingdom 212 bar maker; beloved Hershey's Chocolate World, chocolate- traditional paradigm and capture more of the revenue from Japan 124 Top Flavour Pairings 228 themed hotels and classic old-world cafes serving famous the chocolate trade domestically. -
About Theobroma Cacao
Overview 3 Chocolate Trees: All About Theobroma Cacao How much water does a cacao tree need? Sources Cacao trees cannot survive in very dry International Cocoa Or- weather and thrive in climates with high ganization. www.icco.org/ humidity and rainfall. These moisture-lov- about/growing.aspx ing plants grow best in areas that receive approximately 60 to 80 inches (1,500 to American Museum of 2,000 mm) of rain per year and cannot sur- Natural History. vive in regions that consistently receive www.amnh.org/ less than four inches (100 mm) of rain per sciencebulletins/ month. biobulletin/biobulletin/ story720.html How many beans are needed to make chocolate? American Museum of On average: Natural History. • Each tree will yield 20 to 30 pods per www.amnh.org/ year. sciencebulletins/ • Each pod contains 20 to 40 beans. biobulletin/biobulletin/ • Approximately 400 beans are required story669.html to make one pound of chocolate. by their natural predators, but on an World Agroforestry Centre. How much space does a cacao tree need open plantation they can decimate a www.worldagroforestry. to grow? cacao crop. org/ Cacao trees grow 15 to 25 feet (4.5 – 6 m) • Rainforests meet the shade require- tall and need an area of about 15 square ments of cacao trees and provide the feet (1.4 square meters). high humidity, rainfall and soil condi- tions necessary for continued growth. What kind of soil is best? Cacao trees grown in the shade and Cacao needs soil that is nutrient-rich, humidity of a forest produce far more retains water well and has good drainage.