Metabolite Standards Detected by UHPLC-HRMS

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Metabolite Standards Detected by UHPLC-HRMS Metabolite standards detected by UHPLC-HRMS #ID NAME 1 - 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE 2 - 10-HYDROXYDECANOATE 3 - 1-HYDROXY-2-NAPHTHOATE 4 - 2,4-DIHYDROXYPTERIDINE 5 - 2,6-DIHYDROXYPYRIDINE 6 - 2-Sulfoaniline 7 - 3-AMINO-4-HYDROXYBENZOATE 8 - 3-AMINO-5-HYDROXYBENZOATE 9 - 3-Hydroxyphenyl acetate 10 - 3-METHYL-2-OXINDOLE 11 - 3-NITRO-L-TYROSINE 12 - 4-QUINOLINECARBOXYLATE 13 - 4-QUINOLINECARBOXYLIC ACID 14 - ANILINE-2-SULFONATE (2-SULFOANILINE) 15 - BIS(2-ETHYLHEXYL)PHTHALATE 16 - CORTISOL 21-ACETATE 17 - delta-Valerolactone 18 - DEOXYCORTICOSTERONE ACETATE 19 - DIDECANOYL-GLYCEROPHOSPHOCHOLINE 20 - D-MANNOSAMINE 21 - ETHYL 3-INDOLEACETATE 22 - GALACTOSAMINE 23 - GLUCOSAMINATE 24 - Hydrocortisone acetate 25 - Hydrocortisone acetate (CORTISOL 21-ACETATE) 26- LUMICHROME 27 - Methyl vallinic acid 28 - N6-(DELTA2-ISOPENTENYL)-ADENINE 29 - N-ACETYLPROLINE 30 - N-METHYLGLUTAMATE 31- PALATINOSE 32 - S-HEXYL-GLUTATHIONE 33 - SN-GLYCERO-3-PHOSPHOCHOLINE 34 - URACIL 5-CARBOXYLATE 35 HMDB0000001 1-METHYL-L-HISTIDINE 36 HMDB0000012 DEOXYURIDINE 37 HMDB0000014 DEOXYCYTIDINE 38 HMDB0000015 CORTEXOLONE 39 HMDB0000017 4-pyridoxic acid 40 HMDB0000020 p-hydroxyphenylacetic acid 41 HMDB0000022 3-METHOXYTYRAMINE 42 HMDB0000026 ureidopropionic acid 43 HMDB0000030 BIOTIN 44 HMDB0000033 CARNOSINE 45 HMDB0000034 ADENINE 46 HMDB0000036 Taurocholic acid (TCA) 47 HMDB0000038 DIHYDROBIOPTERIN 48 HMDB0000043 BETAINE 49 HMDB0000045 ADENOSINE MONOPHOSPHATE (AMP) Inselspital Printed on 30.05.2018 University Institute of Clinical Chemistry Page 1/9 Bern, Switzerland Metabolite standards detected by UHPLC-HRMS #ID NAME 50 HMDB0000048 MELIBIOSE 51 HMDB0000050 ADENOSINE 52 HMDB0000052 Argininosuccinic acid 53 HMDB0000053 Androstenedione 54 HMDB0000058 CYCLIC AMP 55 HMDB0000062 L-CARNITINE 56 HMDB0000063 CORTISOL 57 HMDB0000064 CREATINE 58 HMDB0000068 EPINEPHRINE 59 HMDB0000070 pipecolic acid 60 HMDB0000073 DOPAMINE 61 HMDB0000076 DIHYDROURACIL 62 HMDB0000085 DEOXYGUANOSINE 63 HMDB0000089 CYTIDINE 64 HMDB0000094 Citric acid 65 HMDB0000095 CYTIDINE MONOPHOSPHATE 66 HMDB0000099 L-CYSTATHIONINE 67 HMDB0000101 DEOXYADENOSINE 68 HMDB0000107 GALACTITOL 69 HMDB0000112 gamma-aminobutyric acid 70 HMDB0000118 Homovanillic acid 71 HMDB0000121 Folic acid 72 HMDB0000122 D-Glucose 73 HMDB0000125 GLUTATHIONE 74 HMDB0000126 GLYCEROL 3-PHOSPHATE 75 HMDB0000127 D-Glucuronic acid 76 HMDB0000128 Guanidoacetic acid 77 HMDB0000130 homogentisic acid 78 HMDB0000132 GUANINE 79 HMDB0000133 GUANOSINE 80 HMDB0000134 fumaric acid 81 HMDB0000138 Glycocholic acid (GCA) 82 HMDB0000143 D-GALACTOSE 83 HMDB0000148 L-Glutamic acid 84 HMDB0000150 Gluconolactone 85 HMDB0000151 beta-estradiol 86 HMDB0000152 Gentisic acid 87 HMDB0000156 L-Malic acid 88 HMDB0000157 Hypoxanthine 89 HMDB0000158 L-TYROSINE 90 HMDB0000159 L-PHENYLALANINE 91 HMDB0000162 L-Proline 92 HMDB0000163 D-MALTOSE 93 HMDB0000167 L-THREONINE 94 HMDB0000168 L-ASPARAGINE 95 HMDB0000169 D-Mannose 96 HMDB0000172 L-ISOLEUCINE 97 HMDB0000175 Inosinic acid 98 HMDB0000176 maleic acid Inselspital Printed on 30.05.2018 University Institute of Clinical Chemistry Page 2/9 Bern, Switzerland Metabolite standards detected by UHPLC-HRMS #ID NAME 99 HMDB0000177 L-HISTIDINE 100 HMDB0000181 L-DOPA 101 HMDB0000182 L-Lysine 102 HMDB0000186 Alpha-LACTOSE 103 HMDB0000191 L-Aspartic acid 104 HMDB0000192 L-CYSTINE 105 HMDB0000194 ANSERINE 106 HMDB0000195 INOSINE 107 HMDB0000197 Indoleacetic acid 108 HMDB0000201 L-Acetylcarnitine (C2) 109 HMDB0000202 methylmalonic acid 110 HMDB0000207 Oleic acid 111 HMDB0000208 Oxoglutaric acid 112 HMDB0000210 PANTOTHENIC ACID 113 HMDB0000211 MYOINOSITOL 114 HMDB0000212 N-ACETYLGALACTOSAMINE 115 HMDB0000215 N-ACETYL-D-GLUCOSAMINE 116 HMDB0000216 norepinephrine (NORADRENALINE) 117 HMDB0000220 Palmitic acid 118 HMDB0000222 L-PALMITOYLCARNITINE (C16) 119 HMDB0000226 orotic acid 120 HMDB0000227 mevalonic acid 121 HMDB0000229 Nicotinamide ribotide 122 HMDB0000230 N-Acetylneuraminic acid 123 HMDB0000234 Testosterone 124 HMDB0000239 PYRIDOXINE 125 HMDB0000244 RIBOFLAVIN 126 HMDB0000247 SORBITOL 127 HMDB0000248 THYROXINE 128 HMDB0000251 TAURINE 129 HMDB0000252 Sphingosine 130 HMDB0000254 succinic acid 131 HMDB0000258 SUCROSE 132 HMDB0000262 Thymine 133 HMDB0000265 LIOTHYRONINE 134 HMDB0000267 pyroglutamic acid 135 HMDB0000269 SPHINGANINE 136 HMDB0000271 SARCOSINE 137 HMDB0000273 THYMIDINE 138 HMDB0000283 D-RIBOSE 139 HMDB0000286 URIDINE DIPHOSPHATE GLUCOSE 140 HMDB0000288 URIDINE 5'-MONOPHOSPHATE 141 HMDB0000289 Uric acid 142 HMDB0000290 URIDINE DIPHOSPHATE-N-ACETYLGLUCOSAMINE 143 HMDB0000291 Vanillylmandelic acid 144 HMDB0000292 XANTHINE 145 HMDB0000296 URIDINE 146 HMDB0000299 XANTHOSINE 147 HMDB0000300 URACIL Inselspital Printed on 30.05.2018 University Institute of Clinical Chemistry Page 3/9 Bern, Switzerland Metabolite standards detected by UHPLC-HRMS #ID NAME 148 HMDB0000301 urocanic acid 149 HMDB0000303 TRYPTAMINE 150 HMDB0000304 URIDINE DIPHOSPHATE-N-ACETYLGALACTOSAMINE 151 HMDB0000306 TYRAMINE 152 HMDB0000310 Methylacetoacetic acid 153 HMDB0000318 3,4-DIHYDROXYPHENYLGLYCOL 154 HMDB0000355 3-Hydroxymethylglutaric acid 155 HMDB0000397 2-Pyrocatechuic acid 156 HMDB0000418 18-Hydroxycortisol 157 HMDB0000422 2-METHYLGLUTARIC ACID 158 HMDB0000426 Citramalic acid 159 HMDB0000446 N-ALPHA-ACETYLLYSINE 160 HMDB0000448 adipic acid 161 HMDB0000450 5-HYDROXYLYSINE 162 HMDB0000462 ALLANTOIN 163 HMDB0000469 5-HYDROXYMETHYLURACIL 164 HMDB0000472 5-Hydroxy-L-tryptophan 165 HMDB0000479 3-Methyl-L-histidine 166 HMDB0000482 Caprylic acid 167 HMDB0000491 3-methyl-2-oxovaleric acid 168 HMDB0000497 5,6-Dihydrouridine 169 HMDB0000500 4-hydroxybenzoic acid 170 HMDB0000508 RIBITOL 171 HMDB0000510 aminoadipic acid 172 HMDB0000512 N-Acetyl-L-phenylalanine 173 HMDB0000517 L-ARGININE 174 HMDB0000518 chenodeoxycholic acid 175 HMDB0000526 5a-Tetrahydrocortisol 176 HMDB0000528 4,5-Dihydroorotic acid 177 HMDB0000532 Acetylglycine 178 HMDB0000562 CREATININE 179 HMDB0000573 Elaidic acid 180 HMDB0000575 DL-Homocystine 181 HMDB0000582 3,5-Diiodothyronine 182 HMDB0000592 GLUCOSAMINE 6-SULFATE 183 HMDB0000593 PC(18:1/18:1) 184 HMDB0000618 D-Ribulose 5-phosphate 185 HMDB0000619 Cholic acid (CA) 186 HMDB0000625 gluconic acid 187 HMDB0000626 deoxycholic acid 188 HMDB0000630 Cytosine 189 HMDB0000631 Deoxycholic acid glycine conjugate (GDCA) 190 HMDB0000634 Citraconic acid 191 HMDB0000637 Chenodeoxycholic acid glycine conjugate (GCDCA) 192 HMDB0000639 Galactaric acid 193 HMDB0000641 L-Glutamine 194 HMDB0000645 GALACTOSE 1-PHOSPHATE 195 HMDB0000651 Decanoylcarnitine (C10) 196 HMDB0000660 D-Fructose Inselspital Printed on 30.05.2018 University Institute of Clinical Chemistry Page 4/9 Bern, Switzerland Metabolite standards detected by UHPLC-HRMS #ID NAME 197 HMDB0000661 glutaric acid 198 HMDB0000669 Ortho-Hydroxyphenylacetic acid 199 HMDB0000673 Linoleic acid 200 HMDB0000682 INDOXYL SULFATE 201 HMDB0000684 L-Kynurenine 202 HMDB0000687 L-Leucine 203 HMDB0000688 Isovalerylcarnitine (C5) 204 HMDB0000694 L-2-Hydroxyglutaric acid 205 HMDB0000696 L-Methionine 206 HMDB0000698 Lithocholic acid glycine conjugate (GLCA) 207 HMDB0000703 mandelic acid 208 HMDB0000705 Hexanoylcarnitine (C6) 209 HMDB0000707 4-hydroxyphenylpyruvic acid 210 HMDB0000708 Glycoursodeoxycholic acid (GUDCA) 211 HMDB0000714 hippuric acid 212 HMDB0000715 kynurenic acid 213 HMDB0000716 L-pipecolic acid 214 HMDB0000719 L-Homoserine 215 HMDB0000722 Lithocholyltaurine (TLCA) 216 HMDB0000725 4-Hydroxyproline 217 HMDB0000733 Hyodeoxycholic acid 218 HMDB0000742 HOMOCYSTEINE 219 HMDB0000752 Methylglutaric acid 220 HMDB0000755 Hydroxyphenyllactic acid 221 HMDB0000761 Lithocholic acid (LCA) 222 HMDB0000763 5-Hydroxyindoleacetic acid 223 HMDB0000765 MANNITOL 224 HMDB0000766 N-Acetyl-L-Alanine 225 HMDB0000774 PREGNENOLONE SULFATE 226 HMDB0000784 azelaic acid 227 HMDB0000791 L-Octanoylcarnitine (C8) 228 HMDB0000792 sebacic acid 229 HMDB0000802 PTERIN 230 HMDB0000805 Pyrrolidonecarboxylic acid 231 HMDB0000806 myristic acid 232 HMDB0000812 n-acetyl-l-aspartic acid 233 HMDB0000819 NORMETANEPHRINE 234 HMDB0000824 Propionylcarnitine (C3) 235 HMDB0000827 Stearic acid 236 HMDB0000842 quinaldic acid 237 HMDB0000848 Stearoylcarnitine (C18) 238 HMDB0000857 Pimelic acid 239 HMDB0000858 monomethyl glutaric acid 240 HMDB0000870 HISTAMINE 241 HMDB0000873 4-METHYLCATECHOL 242 HMDB0000874 Tauroursodeoxycholic acid (TUDCA) 243 HMDB0000875 TRIGONELLINE 244 HMDB0000881 Xanthurenic acid 245 HMDB0000883 L-VALINE Inselspital Printed on 30.05.2018 University Institute of Clinical Chemistry Page 5/9 Bern, Switzerland Metabolite standards detected by UHPLC-HRMS #ID NAME 246 HMDB0000893 suberic acid 247 HMDB0000896 Taurodeoxycholic acid (TDCA) 248 HMDB0000902 NAD 249 HMDB0000904 Citrulline 250 HMDB0000905 DEOXYADENOSINE MONOPHOSPHATE 251 HMDB0000929 L-TRYPTOPHAN 252 HMDB0000930 TRANS-CINNAMic acid 253 HMDB0000939 S-ADENOSYLHOMOCYSTEINE 254 HMDB0000946 Ursodesoxycholic acid 255 HMDB0000951 Taurochenodesoxycholic acid (TCDCA) 256 HMDB0000954 trans-ferulic acid 257 HMDB0000962 LIPOAMIDE 258 HMDB0000975 TREHALOSE 259 HMDB0001015 N-FORMYL-L-METHIONINE 260 HMDB0001065 2-Hydroxyphenethylamine 261 HMDB0001072 COENZYME Q10 262 HMDB0001078 MANNOSE 6-PHOSPHATE 263 HMDB0001123 2-Aminobenzoic acid 264 HMDB0001129 N-ACETYLMANNOSAMINE 265 HMDB0001138 n-acetylglutamic acid 266 HMDB0001149 5-aminolevulinic acid 267 HMDB0001151 ALLOSE 268 HMDB0001160 tricosanoic acid 269 HMDB0001161 4-Trimethylammoniobutanoic acid 270 HMDB0001173 5'-Methylthioadenosine 271 HMDB0001178 ADENOSINE DIPHOSPHATE RIBOSE 272 HMDB0001186 N1-ACETYLSPERMINE 273 HMDB0001190
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    Mechanism of excessive purine biosynthesis in hypoxanthine- guanine phosphoribosyltransferase deficiency Leif B. Sorensen J Clin Invest. 1970;49(5):968-978. https://doi.org/10.1172/JCI106316. Research Article Certain gouty subjects with excessive de novo purine synthesis are deficient in hypoxanthineguanine phosphoribosyltransferase (HG-PRTase [EC 2.4.2.8]). The mechanism of accelerated uric acid formation in these patients was explored by measuring the incorporation of glycine-14C into various urinary purine bases of normal and enzyme-deficient subjects during treatment with the xanthine oxidase inhibitor, allopurinol. In the presence of normal HG-PRTase activity, allopurinol reduced purine biosynthesis as demonstrated by diminished excretion of total urinary purine or by reduction of glycine-14C incorporation into hypoxanthine, xanthine, and uric acid to less than one-half of control values. A boy with the Lesch-Nyhan syndrome was resistant to this effect of allopurinol while a patient with 12.5% of normal enzyme activity had an equivocal response. Three patients with normal HG-PRTase activity had a mean molar ratio of hypoxanthine to xanthine in the urine of 0.28, whereas two subjects who were deficient in HG-PRTase had reversal of this ratio (1.01 and 1.04). The patterns of 14C-labeling observed in HG-PRTase deficiency reflected the role of hypoxanthine as precursor of xanthine. The data indicate that excessive uric acid in HG-PRTase deficiency is derived from hypoxanthine which is insufficiently reutilized and, as a consequence thereof, catabolized inordinately to uric acid. The data provide evidence for cyclic interconversion of adenine and hypoxanthine derivatives. Cleavage of inosinic acid to hypoxanthine via inosine does […] Find the latest version: https://jci.me/106316/pdf Mechanism of Excessive Purine Biosynthesis in Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency LEIF B.
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