Infectious Diseases Combatting malaria, dengue and Zika using nuclear technology By Nicole Jawerth and Elodie Broussard A male Aedes species mosquito. iseases such as malaria, dengue and transcription–polymerase chain reaction (Photo: IAEA) Zika, spread by various mosquito (RT–PCR) (see page 8). Experts across the Dspecies, are wreaking havoc on millions of world have been trained and equipped by lives worldwide. To combat these harmful the IAEA to use this technique for detecting, and often life-threatening diseases, experts tracking and studying pathogens such as in many countries are turning to nuclear and viruses. The diagnostic results help health nuclear-derived techniques for both disease care professionals to provide treatment and detection and insect control. enable experts to track the viruses and take action to control their spread. Dengue and Zika When a new outbreak of disease struck in The dengue virus and Zika virus are mainly 2015 and 2016, physicians weren’t sure of spread by Aedes species mosquitoes, which its cause, but RT–PCR helped to determine are most common in tropical regions. In most that the outbreak was the Zika virus and not cases, the dengue virus causes debilitating another virus such as dengue. RT–PCR was flu-like symptoms, but all four strains of the used to detect the virus in infected people virus also have the potential to cause severe, throughout the epidemic, which was declared life-threatening diseases. In the case of the Zika a public health emergency of international virus, many infected people are asymptomatic concern by the World Health Organization or only have mild symptoms; however, the virus (WHO) in January 2016. During that time, can cause serious birth defects in newborns, and many countries received support from the it can lead to a debilitating neurological disorder IAEA, in cooperation with the Food and in some adults. Agriculture Organization of the United Nations (FAO), in using this method. One of the most accurate and widely used laboratory methods for detecting While the ongoing Zika threat has been the dengue and Zika viruses is reverse manageable since the epidemic ended in 22 | IAEA Bulletin, June 2020 Infectious Diseases November 2016, dengue has continued birth control method called the sterile insect to be a growing problem. Some countries technique (SIT) (see The Science box). in Latin America and the Caribbean, for example, reported increases of up to three “SIT has been successfully implemented times the number of dengue cases in January against numerous insect pests of agricultural 2020 compared to the same period in 2019. importance and is now being adapted for Meanwhile, in Asia, over 80 000 cases were use against mosquitoes,” said Rafael Argilés reported in Bangladesh in 2019, making it the Herrero, entomologist at the Joint FAO/IAEA country’s largest dengue outbreak on record. Division of Nuclear Techniques in Food and Agriculture. “The method is very specific to The situation has been further compounded the target species and has no impact on other by the global COVID-19 pandemic that living organisms or the environment.” began in early 2020. “The combination of dengue and COVID-19 has overburdened Research on the use of SIT against Aedes many health care systems,” said Diana mosquitoes has intensified worldwide, in part Paez, Head of the Nuclear Medicine and through IAEA and FAO support. Examples Diagnostic Imaging Section at the IAEA. include a four-year project launched in 2016 “This is further exacerbated by dengue and to help countries in the Latin America and the COVID-19 sharing similar symptoms and Caribbean region. In 2019, upon Bangladesh’s some laboratory features, making differential request, a four-year work plan was agreed for diagnosis difficult. When one disease is testing SIT for suppressing dengue-spreading misdiagnosed as the other, it complicates mosquitoes. Projects have also been launched disease management and control, which is in Asia and Europe, and pilot tests have been why accurate tests like RT–PCR are critical.” initiated in 13 countries worldwide, with some achieving as high as 95% suppression. Besides diagnosing and tracking these diseases in people, experts have been looking In early 2020, the IAEA, FAO, Special for ways to reduce the virus-spreading Aedes Programme for Research and Training in mosquito population. One option has been Tropical Diseases (TDR) and WHO released area-wide insect management using, among the Guidance Framework for Testing the other techniques, a nuclear-based insect Sterile Insect Technique as a Vector Control (Photo: IAEA) IAEA Bulletin, June 2020 | 23 Infectious Diseases Tool against Aedes-Borne Diseases. The of Foreign Affairs. He explained how the publication outlines how an SIT programme number of reported malaria cases has dropped can be initiated and how to decide whether to in Oman in the last two decades and the implement it in a country’s affected areas. significant role molecular diagnostic imaging methods, acquired through the IAEA’s technical cooperation programme, play in the Malaria country’s new policy to screen travellers from Malaria is an infectious parasitic disease spread malaria-endemic places. “This policy will by female Anopheles mosquitoes. This disease eventually lead to Oman becoming a malaria- threatens approximately half of the world’s free country.” “Diagnostic imaging is population by causing a range of adverse health able to detect malaria symptoms and, in some cases, death. By reducing the population of Anopheles mosquitoes using SIT, experts are also infections that have not Health professionals can diagnose malaria hoping to reduce the spread of malaria. Some been detected through by testing a patient’s blood for microscopic technical challenges they face have been routine screenings.” signs of the parasite, as well as by measuring ensuring only male sterilized mosquitoes antigens from the immune system’s response are released, as well as developing efficient — Hadj Slimane Cherif, Head, Office to the parasite. Polymerase chain reaction trapping systems. This has created obstacles of Peaceful Nuclear Technology, (PCR) testing is also able to detect malaria, to the large-scale use of SIT against this type Ministry of Foreign Affairs, Oman particularly in cases with low levels of of mosquito. parasites or when other infections are present. Through medical imaging techniques One of the ongoing challenges with SIT such as X-ray and computed tomography for mosquito control is how to effectively (CT), physicians can evaluate the clinical release these fragile insects. In June 2020, complications of the disease. researchers found that using drones to release sterilized males was more cost-effective, “Diagnostic imaging is able to detect malaria faster and less damaging to the mosquitoes infections that have not been detected through than other common release methods, such as routine screenings,” said Hadj Slimane on-the-ground or by airplane. These findings Cherif, Head of the Office of Peaceful marked a major breakthrough in expanding Nuclear Technology of Oman’s Ministry the use of SIT against mosquitoes. THE SCIENCE Sterile Insect Technique The sterile insect technique (SIT) uses radiation to sterilize male insects that are mass produced in special facilities. Large quantities of the sterile male insects are systematically released from the ground or by air. They mate with wild females in nature, which results in no new offspring. Over time, this brings the insect population down, or, when insect populations are isolated, it can eradicate an entire population. SIT has been successfully used for over 50 years against agricultural insect pests. Mass-rearing of insects Male and female insects are The sterile male insects They compete These females lay eggs that are takes place in special separated. Ionizing radiation is are released. with wild males to mate infertile and bear no ospring, facilities. used to sterilize the male insects. with females. reducing the insect population. (Graphic: R. Kenn/IAEA) 24 | IAEA Bulletin, June 2020.