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Abreviations ABREVIATIONS ADN acide désoxyribonucléique Adeno Adenovirus AlHV1 Alcelaphine herpesvirus 1 ARf6 ADP-ribosylation factor ARN acide ribonucléique ARN-T ARN de transfert ATP adénosine triphosphate BECs cellules endothéliales matures du sang BHK-21 baby hamster kidney 21 BL lymphome de Burkitt BoHV-1 bovine herpesvirus-1 BoHV-4 bovine herpesvirus-4 Cbl Casitas B-lineage Lymphoma protein CBP chemokine binding protein CCPs clathrin-coated pits CCSC C Capsid Specific Component CD cluster of differenciation CIEBV Chromosome Integration of Epstein Barr Virus genome CME clathrin-mediated endocytosis CMH complexe majeur d'histocompatibilité CMV cytomegalovirus CPNE copine CryoEM Cryo-électrotomography Cryo-ET Cryo-Electron-Tomography DHC dynein heavy chain DIC dynein internediate chain DLBCL lymphome diffus à larges cellules B DLC dynein light chain DMEM dubelcco's modified eagle's medium DNA Desoxyribonucleic acid EBNA Epstein Barr nuclear antigen EBV Epstein Barr Virus Ecs cellules endothéliales EE early endosomes EHV-2 Equid Herpesvirus-2 EphA2 Ephrine A2 ESCRT endosomal sorting complexes required for transport FCS fetal calf serum FG Phénylalanine-Gly GA appareil de Golgi GAGs glycosaminoglycans GAM goat anti-mouse GEEC GPI-anchored anriched endocytic compartments HCMV human cytomegalovirus 1 ABREVIATIONS HHV-1 Human herpesvirus-1 HHV-4 Human herpesvirus-4 HHV-5 Human herpesvirus-5 HHV-6 Human herpesvirus 6 HHV-7 Human herpesvirus 7 HHV-7 Human herpesvirus 7 HHV-8 Human herpesvirus-8 HIV Human immunodeficiency virus HL lymphome de Hodgkin HLA Human Leucocyte Antigen HPF homologous protein family HPV-16 Human papillomavirus 16 HS héparans sulfates HSV-1 Herpes simplex virus 1 HSV-2 Herpes simplex virus 2 HVEM Herpesvirus entry mediator HVS Herpesvirus saimiri IBD Importin Binding Domain Ics cytokines inflammatoires ICTV International Committee on Taxonomy of Viruses IE gene immediate-early gene IFN interferon IL interleukine ILV intraluminal vesicles Impα Importine α Impβ Importine β INM Inner nuclear membrane IRAK-1 Interleukin-1 receptor activated kinase-1 IRL Internal repeat long KHC Kinesin heavy chain KLC Kinesin light chain KS Kaposi sarcoma KSHV Kaposi sarcoma associated virus L gene late gene LAMP lysosomal-associated membrane protein LANA latency associated nuclear antigen LCMV lymphocytic choriomeningitis virus LE late endosomes LECs cellules endothéliales lymphatiques LMP Latent membrane protein LUR longue région unique linéaire Ly lysosome mAb monoclonal antibody MAPK Mitogen-activated protein kinase 2 ABREVIATIONS MCD maladie multicentrique de Castleman MCF malignant catarrhal fever MCMV Murine cytomegalovirus MCP Major capsid protein ME maturing endosomes ME microscopie electronique MFSD major facilitator superfamily domain containing miRNAs micros RNAs MOCT Microtubule Organizing Center mPy Mouse polyomavirus MuHV-1 Murid herpesvirus-1 MuHV-4 Murid Herpesvirus-4 NAT8 N-acetyltransferase 8 NEC Nuclear Egress Complex NES Nuclear Export Signal NLSs signaux de localisation nucléaire NPCs carcinomes nasopharyngés NTRs Nuclear Transport Receptors Nups nucléoporines OBP Origin-binding protein ONM Outer nuclear membrane ORF open reading frame Ori Origin gene p.f.u plate forming unit p.i post -infection PAC Packaging sequence PAMP’s Pathogen Associated Molecular Patterns PBCH hyperplasie à cellules B polymorphiques PBL lymphome plasmoblastique PCR Polymerase chain reaction PEL lymphome primaire à effusion PH hyperplasie plasmocytaire PIDD induced protein with a death domain PKD protéine kinase D PN pores nucléaires Poms pores membranaires prDNA poly-repetitive DNA PRR pattern-recognition receptors PRV pseudorabiesvirus RAIDD RIP-associated Ich-1/Ced-3 homologous protein with a death domain RE recycling endosomes RE réticulum endoplasmique RNA ribonucleic acid RRV Rhesus monkey rhadinovirus 3 ABREVIATIONS RTA replication and transcription activator SaHV-2 Samiriine herpesvirus-2 SC spindle cells SFV Semliki Forest virus SIDA syndrome de l'immunodéficience acquise siRNA small interference RNA SV40 simian virus 40 TAF TBP-associated factors TBP TATA binding protein TF transcription factor TFIID transcription factor II D TGN Trans-Golgi network TLR Toll-like receptor TR terminal repeats TRI-1 triplex monomer protein TRI-2 triplex dimer protein TRIM tripartite motif TRL long inverted repeat UL Long unique region vCCLs chémokines virales v-FLIP viral FLICE inhibitory protein vGPCR récepteur viral couplé à la protéine G VIH Virus de l'immunodéficience acquise VP Viral protein VSV Vesicular stomatis virus VZV Varicella zona virus 4 REMERCIEMENTSREMERCIEMENTS Je ne sais pas par où commencer…. Il s’est passé tant de choses. Il y a presque 6 ans, je contactais le Prof. Vanderplasschen qui souhaitait agrandir ses équipes et très vite, j’ai pu intégrer celle du Prof. Gillet. Je vais donc d’abord te remercier Laurent : pour ton humanité, ta gentillesse, ta présence, ton soutien dans les moments difficiles, professionnels ou privés. Ensuite, qui d’autre que toi m’aurait aussi bien guidée dans ce travail, orientée, suivie… . Ensuite, je souhaite remercier mes collègues, scientifiques, secrétaires ou techniciens. Les semaines entières partagées sous la hotte avec François, plus de 90.000 puits à infecter, à marquer, à fixer… . Les campagnols à dompter avec Sylvie, les lapins de Béné tous les vendredis pendant des semaines, les nuits à réveiller Maygane qui dormait dans le couloir pendant que je revenais récolter mes puits et qu’elle manipait toutes les deux heures… . Mais au-delà de ça, ce sont des liens affectifs qui se créent, et que la vie dénoue tant les chemins pris s’éloignent forcément. Sylvie, je n’oublierai jamais nos heures passées à LAX, aéroport international de Los Angeles où nous avons séjourné une semaine, coincée à cause du volcan Eyjafjöll en 2010. Les Etats-Unis, la Malaisie puis le Canada : mes plus grands voyages, je les ai faits avec toi ! Merci d’avoir partagé avec moi mon premier congrès international! Bénédicte et Céline, les inséparables : vous avez toujours eu un mot réconfortant en cas de coup dur ; toujours présentes pour un coup de main ou en cas de coup de mou… . Cher Bilal, grâce à toi, les amours pakistanaises n’ont plus de secret pour moi ! Je sais tout ;-) Merci pour ta bonne humeur et dépêche-toi de trouver une femme… parce que tu le vaux bien. Bérengère, on en a partagé des larmes et des joies. Tu t’es éloignée de la Belgique mais j’espère vraiment qu’on fera notre possible pour perdurer cette amitié. Chère Maygane, toutes nos peines de cœur ont été passées à la moulinette, les hommes ont eu souvent les oreilles qui sifflaient avec nous, car on ne les a pas épargnés ! Merci pour tous ces moments d’amitié et de partage (y compris à suer au fitness !!!). Françoise, ton capital sympathie :-D a grandi chaque jour un peu plus dans mon cœur, tu es une fille géniale. Océane, j’espère que tu vas trouver un petit coin de labo au soleil pour accueillir tes deux hommes et toi, merci pour tous tes tuyaux sur le clonage ! Will, mon cher Will. Nous deux, on est devenu amis après le labo : nos rendez-vous hebdomadaires à s’enfiler les escaliers de Bueren en courant, les rendez-vous presque quotidiens sur Whatsapp/par mail/ou sms pour s’encourager dans nos moments difficiles, … tu sais que je ne peux plus me passer de toi ;-). Après ça, il y a tous les anciens, et les nouveaux (que je ne 5 REMERCIEMENTS connais même pas). Je vais en oublier alors je ne me risque pas à tous les citer mais je garde à coup sûr, dans mon cœur, des moments privilégiés passés avec chacun, des échanges sincères, tendres et drôles. Je garderai précieusement ces moments intenses, humains et vrais en souvenir. Mes amis. D’abord Pascal. Mon meilleur ami. Deux jours sans te parler ! C’est une éternité ! Parfois même, tu le sais, on s’appelle 5 fois par jour, et il n’y a pas toujours assez de place sur notre boîte vocale. Tu m’as toujours encouragée à terminer cette thèse, c’est aussi en partie grâce à toi que je n’ai pas abandonné. Marc, Annick, Caroline, Danièle, Vincent, Quentin, Kristel, Cécile, l’autre Cécile, Manu, Valérie, Philippe, Géraldine, Andy, Julien, Catherine, Barbara, Chantal, Nico … il en manque certainement. Mais chacun d’entre vous contribue à ce que je suis aujourd’hui. Et si je ne compte pas mourir avec ma thèse dans les bras, j’espère vivre encore des moments complices et heureux avec vous tous! Merci pour votre soutien, et surtout, pour votre amour. Grâce à vous, je sais qu’on peut aimer très fort en amitié. Ma « nouvelle » filleule Kathleen : à 14 ans, tu m’as choisie comme marraine, merci de ton amour, de ta confiance et aussi, de ton soutien et de tes encouragements. Et puis tous les copains du sport : Flo, Benoit, Christelle, et les 100 autres!!! Tous les lundis, les mardis et les vendredis, je me shoote à votre enthousiasme, au son de vos cris et à votre sourire lors de mes cours. Merci de m’avoir donné la pêche à chaque fois que j’en manquais. Et last but not least, LA FAMILIA !!! Maman, chère maman, ça y est: ta fille sort enfin des études! La plus belle phrase que tu m’offres, c’est quand tu me dis : « Quoique tu décides, je te soutiens, tu feras les bons choix ». Plus on vieillit toutes les deux, plus on se comprend et plus on s’aime. Parrain, marraine et Marie-France : sans vous, je n’aurais certainement pas le parcours que j’ai aujourd’hui : merci pour votre soutien dans toutes les épreuves, et la thèse en faisait partie. Mes cousins : Renaud, super designer, merci pour avoir fait la couverture de ma thèse !! Et Maud, Thomas et Corentin qui ont partagé tant de moments avec moi. Et puis mon Estelle, ma petite fille, à qui j’ai si souvent dit que je ne pouvais pas jouer parce que je devais travailler.
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