bioRxiv preprint doi: https://doi.org/10.1101/2020.09.15.297549; this version posted September 15, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 DWV infection and replication at the early stage in vitro using honey bee pupal 2 cells 3 4 Yunfei Wu, Jing Li, and Tatsuhiko Kadowaki 5 6 Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, 111 Ren'ai 7 Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China 8 9 Corresponding author: 10 Tatsuhiko Kadowaki 11 Department of Biological Sciences, Xi’an Jiaotong-Liverpool University 12 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town 13 Jiangsu Province 215123, China 14 TEL: 86 512 88161659, FAX: 86 512 88161899 15 E-mail:
[email protected] 16 17 Running title: DWV infection and replication in vitro 18 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.09.15.297549; this version posted September 15, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 19 Abstract 20 Deformed wing virus (DWV) has been best characterized among honey bee viruses; 21 however, very little is known about the mechanisms of viral infection and replication 22 due to the lack of honey bee cell lines. To resolve this problem, we established in vitro 23 system to reconstitute DWV binding and entry to the host cell followed by translation of 24 the genome RNA and the polyprotein processing with honey bee pupal cells.