Full wwPDB EM Validation Report ○i

Dec 14, 2020 – 11:42 PM JST

PDB ID : 5WYJ EMDB ID : EMD-6695 Title : Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1) Authors : Ye, K.; Zhu, X.; Sun, Q. Deposited on : 2017-01-13 Resolution : 8.70 Å(reported)

This is a Full wwPDB EM Validation Report for a publicly released PDB entry.

We welcome your comments at [email protected] A user guide is available at https://www.wwpdb.org/validation/2017/EMValidationReportHelp with specific help available everywhere you see the ○i symbol.

The following versions of software and data (see references ○i ) were used in the production of this report:

EMDB validation analysis : 0.0.0.dev61 MolProbity : 4.02b-467 Percentile statistics : 20191225.v01 (using entries in the PDB archive December 25th 2019) Ideal geometry (proteins) : Engh & Huber (2001) Ideal geometry (DNA, RNA) : Parkinson et al. (1996) Validation Pipeline (wwPDB-VP) : 2.15.1 Page 2 Full wwPDB EM Validation Report EMD-6695, 5WYJ

1 Overall quality at a glance ○i

The following experimental techniques were used to determine the structure: ELECTRON MICROSCOPY The reported resolution of this entry is 8.70 Å. Percentile scores (ranging between 0-100) for global validation metrics of the entry are shown in the following graphic. The table shows the number of entries on which the scores are based.

Whole archive EM structures Metric (#Entries) (#Entries) Clashscore 158937 4297 Ramachandran outliers 154571 4023 Sidechain outliers 154315 3826 RNA backbone 4643 859

The table below summarises the geometric issues observed across the polymeric chains and their fit to the map. The red, orange, yellow and green segments of the bar indicate the fraction of residues that contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria respectively. A grey segment represents the fraction of residues that are not modelled. The numeric value for each fraction is indicated below the corresponding segment, with a dot representing fractions <=5% The upper red bar (where present) indicates the fraction of residues that have poor fit to the EM map (all-atom inclusion < 40%). The numeric value is given above the bar.

Mol Chain Length Quality of chain

1 3A 333

2 3B 327

2 3C 327

3 3D 504

4 3E 511

5 3F 573

6 3G 126 Continued on next page... Page 3 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Length Quality of chain

6 3H 126

7 5A 700

8 AA 776

9 AB 643

10 AC 713

11 AD 575

12 AE 1769

13 AF 513

14 AG 896

15 B1 1183

16 BA 923

17 BB 943

18 BC 817

19 BD 594

20 BE 939

21 CA 297

22 CB 1237

23 E1 252

23 E2 252

24 E3 483

25 E4 707

26 K1 316

27 MA 183

28 MB 290

29 MC 593 Continued on next page... Page 4 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Length Quality of chain

30 P1 274

31 R1 367

32 R2 1729

33 S1 489

34 SA 1812

35 SC 255

36 SF 261

37 SG 225

38 SH 236

39 SI 190

40 SJ 200

41 SK 197

42 SM 156

43 SN 143

44 SO 151

45 SP 137

46 SR 143

47 SX 130

48 SY 145

49 SZ 135

50 Sc 82

51 Sd 67

52 Sf 63

53 Sg 152

54 U1 554 Continued on next page... Page 5 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Length Quality of chain

55 U2 250

56 U3 2493

57 U4 189

58 U5 274

59 UA 1615

60 UB 987

61 UC 1033 Page 6 Full wwPDB EM Validation Report EMD-6695, 5WYJ

2 Entry composition ○i

There are 61 unique types of molecules in this entry. The entry contains 173863 atoms, of which 0 are hydrogens and 0 are deuteriums. In the tables below, the AltConf column contains the number of residues with at least one atom in alternate conformation and the Trace column contains the number of residues modelled with at most 2 atoms. • Molecule 1 is a RNA chain called U3 RNA. Mol Chain Residues Atoms AltConf Trace Total C N O P 1 3A 157 0 0 3327 1488 575 1107 157

• Molecule 2 is a protein called rRNA 2’-O-methyltransferase fibrillarin.

Mol Chain Residues Atoms AltConf Trace Total C N O S 2 3B 239 0 0 1866 1183 332 341 10 Total C N O S 2 3C 239 0 0 1866 1183 332 341 10

• Molecule 3 is a protein called Nucleolar protein 56.

Mol Chain Residues Atoms AltConf Trace Total C N O S 3 3D 370 0 0 2915 1843 503 560 9

• Molecule 4 is a protein called Nucleolar protein 58.

Mol Chain Residues Atoms AltConf Trace Total C N O S 4 3E 382 0 0 2935 1859 498 570 8

• Molecule 5 is a protein called Ribosomal RNA-processing protein 9.

Mol Chain Residues Atoms AltConf Trace Total C N O S 5 3F 365 0 0 2916 1871 506 529 10

• Molecule 6 is a protein called 13 kDa ribonucleoprotein-associated protein. Page 7 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O S 6 3G 122 0 0 924 589 159 172 4 Total C N O S 6 3H 122 0 0 924 589 159 172 4

• Molecule 7 is a RNA chain called 5ETS RNA. Mol Chain Residues Atoms AltConf Trace Total C N O P 7 5A 462 0 0 9867 4406 1749 3250 462

• Molecule 8 is a protein called Utp4.

Mol Chain Residues Atoms AltConf Trace Total C N O 8 AA 569 0 0 2845 1707 569 569

• Molecule 9 is a protein called Utp5.

Mol Chain Residues Atoms AltConf Trace Total C N O 9 AB 403 0 0 2015 1209 403 403

• Molecule 10 is a protein called Utp8.

Mol Chain Residues Atoms AltConf Trace Total C N O 10 AC 472 0 0 2360 1416 472 472

• Molecule 11 is a protein called Utp9.

Mol Chain Residues Atoms AltConf Trace Total C N O 11 AD 101 0 0 505 303 101 101

• Molecule 12 is a protein called U3 small nucleolar RNA-associated protein 10,Utp10.

Mol Chain Residues Atoms AltConf Trace Total C N O S 12 AE 1536 0 0 9970 6261 1772 1918 19

• Molecule 13 is a protein called Utp15. Page 8 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O 13 AF 376 0 0 1880 1128 376 376

• Molecule 14 is a protein called Utp17.

Mol Chain Residues Atoms AltConf Trace Total C N O 14 AG 612 0 0 3060 1836 612 612

• Molecule 15 is a protein called Ribosome biogenesis protein BMS1.

Mol Chain Residues Atoms AltConf Trace Total C N O S 15 B1 536 0 0 4325 2801 758 746 20

• Molecule 16 is a protein called Periodic tryptophan protein 2.

Mol Chain Residues Atoms AltConf Trace Total C N O S 16 BA 755 0 0 6026 3862 1025 1123 16

• Molecule 17 is a protein called U3 small nucleolar RNA-associated protein 12.

Mol Chain Residues Atoms AltConf Trace Total C N O S 17 BB 778 0 0 6138 3931 1019 1161 27

• Molecule 18 is a protein called U3 small nucleolar RNA-associated protein 13.

Mol Chain Residues Atoms AltConf Trace Total C N O S 18 BC 783 0 0 6117 3870 1033 1187 27

• Molecule 19 is a protein called U3 small nucleolar RNA-associated protein 18.

Mol Chain Residues Atoms AltConf Trace Total C N O S 19 BD 325 0 0 2539 1606 458 466 9

• Molecule 20 is a protein called U3 small nucleolar RNA-associated protein 21. Page 9 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O S 20 BE 753 0 0 5936 3769 1020 1126 21

• Molecule 21 is a protein called Ribosomal RNA-processing protein 7.

Mol Chain Residues Atoms AltConf Trace Total C N O S 21 CA 196 0 0 1582 1016 259 300 7

• Molecule 22 is a protein called U3 small nucleolar RNA-associated protein 22.

Mol Chain Residues Atoms AltConf Trace Total C N O S 22 CB 1098 0 0 8870 5763 1462 1621 24

• Molecule 23 is a protein called Ribosomal RNA small subunit methyltransferase NEP1.

Mol Chain Residues Atoms AltConf Trace Total C N O S 23 E1 217 0 0 1689 1073 293 312 11 Total C N O S 23 E2 216 0 0 1695 1078 295 313 9

• Molecule 24 is a protein called Essential nuclear protein 1.

Mol Chain Residues Atoms AltConf Trace Total C N O S 24 E3 260 0 0 2114 1378 359 374 3

• Molecule 25 is a protein called Enp2.

Mol Chain Residues Atoms AltConf Trace Total C N O 25 E4 285 0 0 1425 855 285 285

• Molecule 26 is a protein called KRR1 small subunit processome component.

Mol Chain Residues Atoms AltConf Trace Total C N O S 26 K1 175 0 0 1410 903 252 245 10

• Molecule 27 is a protein called U3 small nucleolar ribonucleoprotein protein IMP3. Page 10 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O S 27 MA 133 0 0 1097 692 204 194 7

• Molecule 28 is a protein called U3 small nucleolar ribonucleoprotein protein IMP4.

Mol Chain Residues Atoms AltConf Trace Total C N O S 28 MB 184 0 0 1465 926 273 260 6

• Molecule 29 is a protein called Mpp10,U3 small nucleolar RNA-associated protein MPP10.

Mol Chain Residues Atoms AltConf Trace Total C N O 29 MC 46 0 0 307 193 52 62

• Molecule 30 is a protein called Pre-rRNA-processing protein PNO1.

Mol Chain Residues Atoms AltConf Trace Total C N O S 30 P1 173 0 0 1368 876 245 243 4

• Molecule 31 is a protein called RNA 3’-terminal phosphate cyclase-like protein.

Mol Chain Residues Atoms AltConf Trace Total C N O S 31 R1 355 0 0 2742 1756 466 509 11

• Molecule 32 is a protein called rRNA biogenesis protein RRP5.

Mol Chain Residues Atoms AltConf Trace Total C N O S 32 R2 272 0 0 2228 1433 374 416 5

• Molecule 33 is a protein called Sof1.

Mol Chain Residues Atoms AltConf Trace Total C N O 33 S1 285 0 0 1425 855 285 285

• Molecule 34 is a RNA chain called 18S ribosomal RNA. Page 11 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O P 34 SA 1115 0 0 23759 10623 4225 7796 1115

• Molecule 35 is a protein called 40S ribosomal protein S1-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 35 SC 214 0 0 1709 1084 310 311 4

• Molecule 36 is a protein called 40S ribosomal protein S4-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 36 SF 237 0 0 1881 1205 345 328 3

• Molecule 37 is a protein called 40S ribosomal protein S5.

Mol Chain Residues Atoms AltConf Trace Total C N O S 37 SG 206 0 0 1609 1007 300 299 3

• Molecule 38 is a protein called 40S ribosomal protein S6-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 38 SH 174 0 0 1369 856 262 248 3

• Molecule 39 is a protein called 40S ribosomal protein S7-A.

Mol Chain Residues Atoms AltConf Trace Total C N O 39 SI 165 0 0 1322 856 227 239

• Molecule 40 is a protein called 40S ribosomal protein S8-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 40 SJ 170 0 0 1350 839 268 241 2

• Molecule 41 is a protein called 40S ribosomal protein S9-A. Page 12 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O S 41 SK 175 0 0 1412 892 272 247 1

• Molecule 42 is a protein called 40S ribosomal protein S11-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 42 SM 141 0 0 1143 733 216 191 3

• Molecule 43 is a protein called 40S ribosomal protein S12.

Mol Chain Residues Atoms AltConf Trace Total C N O S 43 SN 124 0 0 890 560 156 172 2

• Molecule 44 is a protein called 40S ribosomal protein S13.

Mol Chain Residues Atoms AltConf Trace Total C N O S 44 SO 134 0 0 1087 698 202 186 1

• Molecule 45 is a protein called 40S ribosomal protein S14-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 45 SP 109 0 0 750 462 147 140 1

• Molecule 46 is a protein called 40S ribosomal protein S16-A.

Mol Chain Residues Atoms AltConf Trace Total C N O 46 SR 125 0 0 973 625 174 174

• Molecule 47 is a protein called 40S ribosomal protein S22-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 47 SX 129 0 0 1021 650 188 180 3

• Molecule 48 is a protein called 40S ribosomal protein S23-A. Page 13 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O S 48 SY 103 0 0 785 501 144 138 2

• Molecule 49 is a protein called 40S ribosomal protein S24-A.

Mol Chain Residues Atoms AltConf Trace Total C N O 49 SZ 101 0 0 801 512 144 145

• Molecule 50 is a protein called 40S ribosomal protein S27-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 50 Sc 79 0 0 595 371 108 111 5

• Molecule 51 is a protein called 40S ribosomal protein S28-A.

Mol Chain Residues Atoms AltConf Trace Total C N O S 51 Sd 63 0 0 497 306 99 91 1

• Molecule 52 is a protein called 40S ribosomal protein S30-A.

Mol Chain Residues Atoms AltConf Trace Total C N O 52 Sf 30 0 0 251 162 50 39

• Molecule 53 is a protein called Ubiquitin-40S ribosomal protein S31.

Mol Chain Residues Atoms AltConf Trace Total C N O S 53 Sg 51 0 0 397 249 73 71 4

• Molecule 54 is a protein called Utp7.

Mol Chain Residues Atoms AltConf Trace Total C N O 54 U1 285 0 0 1425 855 285 285

• Molecule 55 is a protein called Utp11. Page 14 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Mol Chain Residues Atoms AltConf Trace Total C N O 55 U2 73 0 0 365 219 73 73

• Molecule 56 is a protein called Utp20.

Mol Chain Residues Atoms AltConf Trace Total C N O 56 U3 1407 0 0 7035 4221 1407 1407

• Molecule 57 is a protein called rRNA-processing protein FCF1.

Mol Chain Residues Atoms AltConf Trace Total C N O S 57 U4 126 0 0 990 633 179 168 10

• Molecule 58 is a protein called Ribosome biogenesis protein UTP30.

Mol Chain Residues Atoms AltConf Trace Total C N O S 58 U5 248 0 0 2009 1285 357 359 8

• Molecule 59 is a protein called Helical domain protein.

Mol Chain Residues Atoms AltConf Trace Total C N O 59 UA 338 0 0 1690 1014 338 338

• Molecule 60 is a protein called Helical domain protein.

Mol Chain Residues Atoms AltConf Trace Total C N O 60 UB 555 0 0 2775 1665 555 555

• Molecule 61 is a protein called Unassigned helices.

Mol Chain Residues Atoms AltConf Trace Total C N O 61 UC 660 0 0 3300 1980 660 660 Page 15 Full wwPDB EM Validation Report EMD-6695, 5WYJ

3 Residue-property plots ○i

These plots are drawn for all protein, RNA, DNA and oligosaccharide chains in the entry. The first graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and atom inclusion in map density. Residues are color-coded according to the number of geometric quality criteria for which they contain at least one outlier: green = 0, yellow = 1, orange = 2 and red = 3 or more. A red diamond above a residue indicates a poor fit to the EM map for this residue (all-atom inclusion < 40%). Stretches of 2 or more consecutive residues without any outlier are shown as a green connector. Residues present in the sample, but not in the model, are shown in grey. • Molecule 1: U3 RNA

Chain 3A:        G1 U2 C3 G4 A5 C6 G7 U8 A9 C10 U11 U12 C13 A14 U15 G18 A19 A22 U23 U24 U C U A U A G G A A U C G U38 U46 G47 A48 C49 U50 C51 U52 U53 C54 A55 A58 G59 A60 G61 C62 C63 A64 C65 U66 G67 A68    G78 G79 U80 U81 G82 A83 U84 G85 A86 G U C C C A U A A C96 U102 A103 C104 C105 C106 C107 A108 G109 A110 G111 U112 G113 A114 G A A A C C G A A A U U G A A U C U A A A U U A G C U U G G U C C G C A A U C C U U A G C G G U U C G G C C A U C U A U A A U U U U G A A U A A A A A U U U U G C U U U G199 U204    G205 C206 A207 U U U G U A G U U U U U U C C U U U G G A A G U A A U U A C A A U A U242 A246 C252 G253 A254 U255 G256 A257 U260 U261 G262 A263 C264 C265 C266 A267 U C C U      A U G U A C U U C U U U U U U G A A G G G A U A G G G C U C U A U304 G310 G311 A317 U318 G319 A322 G323 U324 C325 U326 G327 A328 C329 A330 A331 G332 U333

• Molecule 2: rRNA 2’-O-methyltransferase fibrillarin

Chain 3B: MET SER PHE ARG PRO GLY SER ARG GLY GLY SER ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY                  SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY ARG GLY GLY ALA ALA GLY GLY ALA ARG GLY GLY A84 K85 I88 E89 P90 A94 G95 A99 R100 G101 K102 L105 L106 V107 T108 K109 N110 M111 A112 P113 G114 E115 S116 V117 Y118 G119 E120 K121 R122 I123 S124 V125 E126                         E127 P128 S129 D132 G133 V134 P135 P136 T137 K138 V139 E140 Y141 R142 V143 W144 N145 R148 S149 K150 L159 D160 F163 I164 A165 K168 K169 V170 L171 Y172 L173 G174 A175 A176 T179 D186 V187 V188 G189 P190 E191 G192 V193 V194 H201 R205 R214 I217 Page 16 Full wwPDB EM Validation Report EMD-6695, 5WYJ                       I221 P227 Y230 R231 M232 L233 I234 G235 M236 V237 D238 C239 V240 F241 A242 D243 V244 A245 R251 L255 M259 F260 L261 K262 D263 Q264 G265 G266 V267 V268 I271 K272 I276 V280 D281 Q291 R294 I298 K299 P300 E310 R311 D312 H313 C314 V317 G318   R319 Y320 M321 R322 SER GLY LEU LYS LYS

• Molecule 2: rRNA 2’-O-methyltransferase fibrillarin

Chain 3C: MET SER PHE ARG PRO GLY SER ARG GLY GLY SER ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY PHE GLY GLY ARG GLY GLY                           SER ARG GLY GLY ALA ARG GLY GLY SER ARG GLY GLY ARG GLY GLY ALA ALA GLY GLY ALA ARG GLY GLY A84 K85 V86 V87 I88 H93 A94 G95 V96 R100 G101 K102 E103 D104 L105 L106 V107 M111 A112 P113 G114 E115 S116 V117 Y118 G119 R122 E126 E127 P128 S129 K130                                         E131 D132 G133 V134 P135 P136 T137 K138 R142 V143 W144 N145 P146 F147 R148 S149 K150 A153 G154 I155 M156 G157 D160 E161 L162 F163 I164 A165 P166 G167 K168 K169 V170 L171 Y172 A175 A176 S177 G178 T179 S180 H183 Y195 A196 V197 E198 F199 S200 H201 R202 P203 G204 L207                                      I208 S209 M210 A211 R214 P215 N216 I217 I218 P219 I220 I221 E222 D223 A224 R225 R231 M232 L233 I234 G235 M236 V237 D238 C239 V240 F241 A242 D243 V244 A245 D248 Q249 A250 R251 I252 I253 N256 S257 H258 M259 F260 L261 K262 D263 G266 V267 V268 I269 S270 A273 N274 C275 I276                       D277 S278 T279 V280 D281 A282 E283 R288 R294 E295 E296 R297 I298 K299 P300 L306 E307 P308 Y309 E310 R311 D312 H313 C314 R319 R322 SER GLY LEU LYS LYS

• Molecule 3: Nucleolar protein 56

Chain 3D:                  MET ALA PRO ILE GLU TYR LEU L8 F9 F18 K19 V20 K21 L22 Q23 S29 R30 L31 K32 E33 V34 Q35 E36 Q37 F41 F44 T45 K46 L47 I48 E49 L50 A54 A62 A66 I69 V74 L78 I81 P87 K88 A89 S90 S91 K92 K93 K94 N95                     I96 P107 E111 Y115 V116 S120 N121 E122 L123 A124 R129 L138 G141 L142 Q143 S144 G145 D146 L147 E148 R149 A150 Q151 L152 G153 L154 G155 H156 A157 Y158 A161 K162 V163 K164 F165 S166 V167 Q168 K169 N170 Q176 A177 I178 A179 L180 W198 Y199 L206                         P211 D212 N213 K218 L221 F222 I223 K224 D225 K226 A227 S228 L229 N230 D231 D232 S233 L234 H235 D236 L237 A238 A239 L240 E243 D244 Q249 D253 R256 I257 S258 M259 G260 Q261 D262 I263 S264 E265 M268 A275 Y284 R285 Y291 H297 E309 T323 N324       L325 S326 K327 Q328 A329 ALA SER THR VAL GLN ILE LEU GLY ALA GLU LYS ALA LEU PHE ARG ALA LEU LYS THR LYS GLY ASN THR PRO LYS TYR GLY LEU ILE TYR HIS SER GLY PHE ILE SER LYS ALA SER ALA K370 G373 R377 I389 D390 N391 Y392 E395 P396 T417 GLY LYS PRO THR LEU LYS ASN GLU LEU ALA ILE GLN GLU ALA MET GLU LEU TYR ASN LYS ASP LYS PRO ALA ALA GLU VAL GLU GLU THR LYS GLU LYS GLU SER SER LYS LYS ARG LYS LEU GLU ASP ASP ASP GLU GLU LYS LYS GLU LYS LYS GLU LYS LYS SER LYS LYS GLU LYS Page 17 Full wwPDB EM Validation Report EMD-6695, 5WYJ LYS GLU LYS LYS GLU LYS LYS ASP LYS LYS GLU LYS LYS ASP LYS LYS GLU LYS LYS ASP LYS LYS LYS LYS SER LYS ASP

• Molecule 4: Nucleolar protein 58

Chain 3E:                      MET ALA Y3 V4 E7 T8 S9 A10 G11 Y12 A13 L14 L15 S18 ASP LYS LYS ILE TYR LYS SER SER SER LEU ILE GLN ASP LEU ASP SER SER ASP LYS V38 L39 K40 I44 A45 A46 F47 S48 K49 F50 N51 S52 A53 A56 L57 E58 E59 A60 N61 S62 I63 I64 E65                                             G66 K67 V68 L72 E73 K74 L75 L76 E77 E78 I79 K80 K81 D82 K83 K84 S85 T86 L87 I88 V89 S90 E91 T92 A95 N96 A97 I98 N99 K100 L101 G102 L103 N104 F105 N106 V107 V108 S109 D110 A111 V112 D115 I116 Y117 R118 P125 E126 L127 L128 P129 G130 M131 S132 L143                       K151 L152 K153 F154 S155 A156 D157 K158 V159 D160 I163 A166 D172 L173 D174 N178 R183 W187 H191 T200 A206 L210 I214 R215 E220 T221 D222 L223 S224 E225 I226 L227 P228 E229 E230 I231 R234 T237 A238 V241 S242 M243 G244 T245               E246 Q249 T250 D251 L252 N256 A257 L272 L276 S277 M280 T288 Q289 G292 E293 G296 L299 I300 A301 S305 A310 K311 A314 I317 K333 H334 D335 T336 L341 L342 L347 V348 G349 G357 K358 R361 V362 L363 A364 A365 K366 A367           Y373 D374 D379 R380 D381 D382 S383 G384 D385 L388 E389 S390 R391 R397 L401 E402 G403 ARG ASP LEU ARG THR THR PRO LYS VAL VAL ARG GLU ALA LYS LYS VAL GLU MET THR GLU ALA ARG ALA TYR ASN ALA ASP ALA ASP THR ALA LYS ALA ALA SER ASP SER GLU SER ASP SER ASP ASP GLU GLU GLU GLU LYS LYS GLU LYS LYS GLU LYS LYS ARG LYS ARG ASP ASP ASP GLU ASP SER LYS ASP SER LYS LYS ALA LYS LYS GLU LYS LYS ASP LYS LYS GLU LYS LYS GLU LYS LYS GLU LYS LYS GLU LYS LYS GLU LYS LYS GLU LYS LYS GLU LYS LYS SER LYS LYS GLU LYS LYS GLU LYS LYS

• Molecule 5: Ribosomal RNA-processing protein 9

Chain 3F: MET SER ASP VAL THR GLN GLN LYS LYS ARG LYS ARG SER LYS GLY GLU VAL ASN PRO SER LYS PRO THR VAL ASP GLU GLU ILE THR ASP PRO SER SER ASN GLU ASP GLU GLN LEU GLU VAL SER ASP GLU GLU ASP ALA LEU GLU SER GLU GLU GLU PHE GLU GLY GLU ASN PRO ALA ASP LYS ARG ARG ARG LEU ALA LYS GLN TYR LEU GLU ASN LEU LYS SER GLU ALA ASN ASP ILE LEU THR ASP ASN ARG ASN ALA GLU GLU LYS ASP LEU ASN ASN LEU LYS GLU ARG THR ILE ASP GLU TYR ASN ASN PHE ASP ALA GLY ASP LEU ASP LYS ASP ILE ILE ALA SER ARG             LEU LYS GLU ASP VAL ALA GLU GLN GLN G130 R134 G137 D138 I142 S143 E144 A145 K146 Q147 V152 G153 E154 N155 N156 L157 T158 C159 I160 S161 C162 P165 VAL LEU ASN LYS TYR THR PHE GLU GLU SER SER ASN GLY ASP LYS ASN LYS GLY R184 A187 Y188 T189 V190                     S191 K192 D193 L194 Q195 L196 T197 K198 Y199 D200 I201 R207 P208 K209 K210 L211 K212 Y213 I221 P222 THR SER LYS HIS GLU TYR GLU N230 T231 T232 E233 G234 H235 Y236 D237 E238 I239 A244 S245 P246 D247 G254 D257 R258 K259 L260 T265 E266 T276 K277 R280 G281                        E282 V283 L284 S285 L286 A287 F288 R289 K290 N291 S292 D293 Q294 L295 Y296 A297 S298 C299 A300 D301 F302 K303 I304 R305 I309 N310 Q311 L315 E316 I317 L318 Y319 H322 D323 I324 V325 I328 S329 A330 L331 A332 M333 E334 R335 R342 D343 R344 T345 A346 M347 L348 W349 K350 I351 P352 Page 18 Full wwPDB EM Validation Report EMD-6695, 5WYJ                    D353 E354 T355 R356 L357 T358 F359 R360 G361 G362 D363 E364 P365 Q366 K367 L368 L369 R370 R371 E375 ASN ALA LYS GLU GLY GLU ASP GLY GLU VAL LYS TYR PRO ASP GLU SER GLU ALA PRO LEU F396 F397 C398 I402 V405 S406 M407 V408 D409 D410 T415 G416 S417 N421        L424 L427 K430 F434 T435 E436 R437 I438 A439 H440 G441 I442 L443 PRO GLU PRO SER PHE ASN ASP ILE SER GLY GLU THR ASP GLU GLU LEU ARG LYS ARG GLN LEU GLN GLY LYS LYS L469 L470 Q471 P472 I475 L478 I481 P482 Y483 S484 N485 S491 W492 S493 G494                     S495 L496 K497 K500 I501 L505 L511 G512 E513 L514 S515 G516 A517 K518 Q525 V526 V527 E528 S529 GLY LYS HIS GLY LYS GLU K536 F537 R538 I539 L540 A541 S542 I543 A544 K545 E546 H547 R548 L549 G550 R551 W552 I553 A554 N555 V556 S557 G562 I563 Y564 S565 A566 V567 Q570 THR GLY PHE

• Molecule 6: 13 kDa ribonucleoprotein-associated protein

Chain 3G:                       MET SER ALA PRO N5 P6 K7 A12 I20 L21 V24 Q25 Q26 A27 A28 N29 Q32 L33 K34 K35 A40 S49 E50 I53 M54 A55 A56 D57 E72 P77 Y78 V81 P82 S83 R84 R89 A90 C91 G92 V93 S94 R95 P96 V97 I98 A99 A100 S101 I102   N105 K111 I114 K118 E122 I126

• Molecule 6: 13 kDa ribonucleoprotein-associated protein

Chain 3H:                       MET SER ALA PRO N5 L11 A12 D13 Q25 N29 L30 R31 Q32 L33 K34 K35 A40 R46 S49 A56 D57 C58 V79 F80 V81 P82 S83 R84 V85 V93 S94 R95 P96 V97 I98 A99 A100 S101 N105 D106 A109 I110 K111 I114 I121 L125  I126

• Molecule 7: 5ETS RNA

Chain 5A: A U G C G A A7 G12 U13 U14 A21 A22 C26 G27 A A A A G A G U U U G G A A A C G A A U U C G A G U A54 U58 U59 G60 U61 C62 G63 G67 U68 U69 A70 U71 G72 U76                    U G U A A A U G G C C U C G90 U91 G98 G101 A102 G103 A106 G107 U108 C109 G114 G115 A118 U119 C120 G121 U122 C123 A124 G125 A126 U127 C128 U129 G130 C131 C132 U133 A134 C137 U138 C139 U140 A141 U142 A143 C144 A145 G146 G150                                             U151 U152 U153 A154 A155 U156 U157 G158 A159 C160 A U162 G163 G164 G168 A169 U170 G171 C172 G173 U174 A175 U176 U177 G178 A179 G180 A181 G182 A183 U184 A185 C186 A187 A188 U189 U190 U191 G192 G193 G194 A195 A196 G197 A198 A199 A200 U201 U202 C203 C204 C205 A206 G207 A208 G209 U210 G211 U212 Page 19 Full wwPDB EM Validation Report EMD-6695, 5WYJ                              G213 U214 U215 U216 C217 U218 U219 U220 U221 G222 C223 G224 U225 U226 U227 A228 A229 U232 G233 A234 A235 C236 A237 G238 U239 C240 U241 C242 A243 U244 C245 G246 G250 C251 A252 U253 C U U G C G A260 U261 U262 C263 C264 A265 U266 U267 G268 G269 U270 G271 A272 G273 C274 A275 G278 A279 A280 G281 G282 U286 G287 G290 G291 A292 U293 U294 A C U A G C U A A U A G C A A U C U A U U U C A A A G A A U U C A A A C U U G333 G334 G337 A338 A339 U340                            G341 C342 U348 G349 U352 A353 G354 C355 C356 G357 G358 U359 C360 G361 C362 A363 A364 G365 A366 C367 U368 G369 U370 G371 A372 U373 U376 U377 C378 A379 A380 G381 U382 G383 U384 A385 A386 C387 C388 U389 C390 C391 U392 C393 U394 C395 A396 A397 A398 U399 C400 A401 G402 C403 G404 A405 U406                               A407 U408 C409 A410 C413 G414 C417 C418 A419 U U C C G U G A A A C A C432 C433 G434 G435 G436 G437 U438 A439 U440 C441 U442 G443 U444 G447 G448 U449 G450 G451 A452 A453 C454 C455 U456 G457 A458 U459 U460 A461 G462 A463 G464 G465 A466 A467 A468 C469               A476 G477 C480 U A U G G U A U G G U G A C494 G495 G496 A497 G502 C503 G506 U507 C508 G511 A512 G513 U514 A517 A518 A519 A520 G521 C522 U523 U524 U525 U526 U527 G528 A529 A530 A534 G535 C538 A539 U542 C C G545                       G546 C547 A548 G552 A553 G554 A555 G556 A557 C558 C559 U560 G561 A562 A A A A G C A A U U U U U575 C576 U577 G578 G579 A580 A581 U582 U583 U589 G590 U591 U U C C A A A C U C A A U A A G U A U C U U C U A G C A A G A G G G A A U A G G U G G G A A A A A A A A A A A G A G A U U U C G G U U U C U U U C U U U U U U A C U G C U U G U U G C U U C U U C U U U U A A G A U A G U

• Molecule 8: Utp4

Chain AA:                        X1 X5 X6 X9 X10 X11 X12 X13 X16 X20 X27 X28 X29 X33 X34 X35 X36 X37 X41 X42 X43 X44 X49 X54 X61 X62 X65 X66 X67 X68 X72 X73 X74 X75 X76 X77 X78 X79 X83 X95 X96 X97 X98 X99 X108                            X109 X110 X111 X116 X129 X132 X136 X137 X140 X143 X144 X148 X152 X153 X154 X155 X156 X159 X160 X161 X165 X166 X167 X172 X173 X178 X179 X180 X181 X186 X187 X193 X194 X195 X202 X203 X204 X205 X213 X214 X215 X216 X217 X218                      X219 X231 X232 X233 X234 X239 X240 X245 X246 X247 X248 X249 X252 X253 X254 X255 X256 X257 X258 X262 X272 X273 X274 X277 X278 X281 X282 X283 X289 X290 X294 X295 X296 X297 X298 X302 X308 X309 X310 X311 X312 X313 X319 X332 X338                        X339 X340 X344 X347 X354 X355 X356 X357 X358 X359 X360 X366 X372 X373 X382 X383 X384 X385 X386 X387 X388 X391 X392 X393 X394 X395 X398 X399 X400 X401 X404 X405 X409 X410 X411 X412 X415 X416 X417 X418 X421 X422 X423 X424 X425 X428 X429                       X433 X434 X438 X439 X444 X452 X460 X461 X462 X463 X464 X465 X466 X475 X476 X477 X478 X479 X482 X483 X484 X485 X486 X492 X493 X498 X499 X500 X501 X502 X506 X507 X516 X517 X518 X519 X522 X523 X524 X529 X530 X533 X534 X535 X536 X537      X538 X539 X553 X554 X561 X565 X569 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK Page 20 Full wwPDB EM Validation Report EMD-6695, 5WYJ UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 9: Utp5

Chain AB:                                X1 X2 X3 X4 X5 X8 X9 X14 X15 X16 X17 X18 X19 X20 X21 X22 X28 X29 X30 X31 X32 X33 X34 X42 X48 X49 X54 X58 X59 X62 X68 X72 X73 X74 X80 X84 X89 X90 X91 X92 X93 X94 X95 X96 X97 X101                                    X102 X103 X104 X108 X109 X110 X114 X115 X116 X117 X121 X125 X131 X132 X133 X134 X135 X136 X137 X138 X139 X140 X141 X142 X145 X148 X149 X153 X154 X155 X160 X161 X167 X177 X180 X181 X182 X185 X186 X190 X191 X192 X193 X194 X195                                X201 X202 X203 X206 X207 X216 X220 X221 X222 X223 X226 X227 X228 X236 X237 X238 X239 X240 X244 X248 X253 X254 X255 X259 X260 X261 X262 X263 X264 X265 X266 X267 X268 X271 X272 X273 X274 X275 X276 X277 X280 X281 X282 X283 X284 X285 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          UNK UNK UNK UNK UNK X414 X428 X429 X430 X431 X432 X433 X434 X435 UNK UNK UNK UNK X440 X441 X442 X446 X452 UNK X454 X464 UNK UNK X467 X473 X474 X477 X478 X479 X480 X484 UNK UNK UNK UNK X489 X508 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK    UNK X520 X547 X548 X549 X550 X553 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 10: Utp8

Chain AC:                                                  X1 X2 X3 X4 X8 X9 X12 X13 X14 X15 X16 X17 X18 X19 X20 X21 X22 X23 X28 X29 X30 X31 X35 X36 X37 X38 X47 X48 X49 X50 X51 X52 X53 X54 X55 X56 X57 X58 X59 X60 X61 X62 X63 X64 X68 X69 X70 X71 X72 X73 X74 X75 X76 X77                                                   X78 X79 X80 X81 X82 X83 X84 X85 X86 X87 X88 X89 X90 X91 X92 X93 X94 X95 X96 X97 X98 X99 X100 X101 X102 X103 X104 X105 X106 X107 X108 X109 X112 X113 X114 X115 X116 X117 X126 X127 X128 X129 X130 X131 X132 X133 X134 X137 X138 X139 X142 X143 X144 X145 X146 X147 Page 21 Full wwPDB EM Validation Report EMD-6695, 5WYJ                                                  X148 X151 X152 X153 X157 X158 X159 X160 X163 X164 X165 X166 X167 X168 X169 X170 X171 X172 X173 X174 X175 X176 X177 X178 X179 X180 X181 X182 X183 X184 X185 X186 X187 X188 X189 X190 X193 X196 X197 X202 X203 X206 X207 X208 X211 X212 X213 X214 X215 X216 X217 X218                                     X219 X226 X227 X228 X231 X235 X236 X237 X238 X239 X240 X243 X244 X245 X246 X247 X248 X249 X250 X253 X254 X255 X256 X257 X258 X261 X262 X263 X264 X265 X266 X267 X268 X269 X270 X274 X275 X276 X277 X278 X279 X280 X281 X284 X285 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK    UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X408 X409 X410 X411              X414 X415 X416 X417 X418 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X436 X439 X440 X443 X447 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X459 X460 X461 X466 X470 X474 X475 X476 UNK UNK UNK X480 X485 X491         X495 UNK UNK UNK UNK X500 X501 X502 X503 X510 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X525 X530 X534 X537 UNK UNK UNK UNK UNK X543 X548 X552 X553 X556 UNK UNK UNK UNK UNK UNK UNK UNK UNK X566 X567 X568 X576               UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X595 X596 X597 X598 X599 X602 X603 X604 X605 X606 X607 X608 X609 X610 X611 X612 X613 X617 X626 UNK UNK UNK UNK UNK UNK X633 X645 X646 X647 X650 X651 X652 UNK UNK UNK UNK UNK   UNK UNK X660 X675 X681 X688 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 11: Utp9

Chain AD: UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK        UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X381 X389 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X407 X408 X409 X410 X411 X412 X415 X420 UNK UNK UNK UNK UNK UNK UNK X428 X439 Page 22 Full wwPDB EM Validation Report EMD-6695, 5WYJ                 UNK X441 X447 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X458 X459 X460 X461 X465 X466 X469 UNK UNK X472 X488 X491 X492 X495 X498 X499 X502 X509 X513 X514 X515 X516 X517 X518 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 12: U3 small nucleolar RNA-associated protein 10,Utp10

Chain AE:               MET SER S3 L8 A9 Q10 V11 A12 S13 N14 N15 L20 D21 R25 H29 S30 A31 S32 L33 S37 A41 T42 Q43 D44 Y45 D46 F47 E58 P64 R71 F74 S75 E76 S77 S78 L81 D82 R83 N84 V85 D95 I98 L104 A105 S106                      W109 L115 H116 A117 T118 E119 W120 L121 V122 R123 R124 F125 Q126 I127 H128 T132 E133 M134 L137 N141 R150 I151 L152 S153 I154 I155 K156 L157 N162 C163 L164 V168 R169 S170 E171 A176 I180 F183 N184 D185 Y192 L196 H202 N203 Y206                   Q209 T213 I218 N219 F223 N227 D228 E229 K230 L231 N232 L245 L246 K249 L261 V262 V263 F264 A265 T266 A267 K272 I275 D287 A288 K292 F303 L306 K307 Q314 L315 P316 I319 F323 D324 S325 K326 L333 T334 F335 L336 D337 K338           E339 P342 V343 C344 D345 K346 F347 I348 T349 S350 Y351 T352 R353 S354 R357 Y358 D359 R360 S361 I365 I366 L367 S368 L369 L370 K371 R374 L375 E376 I384 L390 SER GLU ILE L394 K397 L400 V401 F404 E405 Y406 F407 I408 SER ILE ASN E412 D413 L414 S421               LEU GLY LEU THR GLY GLU LEU PHE GLU ILE ARG LEU THR THR SER LEU PHE THR ASN ALA ASP VAL ASN THR ASP ILE VAL LYS GLN LEU SER ASP PRO VAL GLU THR THR LYS LYS ASP THR A463 S464 F465 Q466 T467 F468 L469 D470 K471 H472 S473 E474 L475 I476 N477 T478 T479 N480                                         L484 T485 E486 T487 G488 E489 R490 V494 I502 G503 K504 G505 Y506 K507 A508 S509 S510 F511 L512 T513 S514 F515 E520 F525 R528 V529 T530 I531 S532 P533 A534 A535 P536 T537 A538 L539 K540 S543 L544 N545 N546 I547 A548 K549 Y550 I551 N552 S553 I554 E555 N559 T562                                    L563 V564 P565 C566 L567 I568 C569 A570 L571 R572 D573 A574 S575 I576 K577 V578 R579 K584 S587 A590 K591 R592 P593 S594 T595 K596 H597 Y598 F599 L600 S601 D602 K603 G606 E607 N608 I611 P612 M613 L614 N615 P616 K617 D618 S619 W622 G625 F626 L627 N628 E629 Y630 V631                                     T632 E633 N634 Y635 D636 I637 S638 R639 I640 L641 T642 P643 K644 E647 K648 F653 W654 A655 N656 Q657 A658 L659 L660 I661 P662 S663 P664 Y665 A666 V669 L670 L671 N675 K676 T679 Y680 A681 S682 S683 Y684 S685 S686 L687 F688 E689 E690 F691 I692 S693 H694 Y695 L696 R699                                     S705 C706 I707 A708 N709 F713 E714 R718 V724 S725 P726 K727 E728 K729 Q730 S731 F732 M733 I734 D735 F736 V737 L738 S739 A740 L741 N742 S743 D744 Y745 E746 Q747 L748 A749 N750 I751 A752 A753 E754 R755 L756 I757 S758 I759 F760 A761 S762 L763 N764 Q767 K768 L769 K770 Q773 N774                                               I775 V776 D777 S778 S779 S780 N781 V782 E783 S784 S785 Y786 D787 T788 V791 L792 Q793 S794 L795 D798 S799 D800 I801 F802 I805 L806 N807 Q808 UNK X810 X811 X812 X813 X814 X815 X816 X817 X818 X819 X820 X821 X822 X823 X824 X825 X826 X827 X828 X829 X830 X831 X832 X833 X834 X835 X836 X837                                                 X843 X846 X847 X848 X849 X850 X851 X852 X853 X854 X855 X858 X861 X862 X863 X864 X865 X866 X869 X870 X871 X872 X873 X874 X875 X876 X877 X878 X879 X880 X885 X886 X890 X891 X892 X893 X894 X895 X896 X897 X898 X899 X900 X901 X906 X907 X908 X909 X910 X911 X912 X913 Page 23 Full wwPDB EM Validation Report EMD-6695, 5WYJ                                                       X914 X915 X916 X917 X918 X919 X920 X921 X922 X923 X924 X925 X926 X927 X928 X929 X930 X931 X932 X933 X934 X935 X936 X937 X938 X939 X942 X943 X944 X947 X948 X949 X950 X951 X952 X953 X954 X955 X956 X957 X958 X959 X960 X961 X962 X963 X964 X965 X966 X967 X968 X969 X970 X971 X972 X973 X974 X975                                                         X976 X977 X978 X979 X980 X983 X984 X985 X986 X987 X988 X989 X990 X991 X992 X993 X994 X995 X996 X997 X998 X999 X1000 X1001 X1002 X1003 X1004 X1005 X1006 X1007 X1008 X1009 X1010 X1011 X1012 X1013 X1014 X1015 X1016 X1017 X1018 X1019 X1020 X1021 X1022 X1023 X1024 X1025 X1026 X1027 X1028 X1029 X1030 X1031 X1032 X1033 X1034 X1035 X1036                                                      X1037 X1041 X1042 X1045 X1046 X1047 X1048 X1049 X1050 X1051 X1052 X1053 X1054 X1055 X1056 X1057 X1058 X1059 X1060 X1061 X1062 X1063 X1064 X1065 X1066 X1067 X1068 X1069 X1070 X1071 X1072 X1073 X1074 X1075 X1076 X1077 X1078 X1079 X1080 X1081 X1082 X1083 X1084 X1085 X1086 X1087 X1088 X1089 X1090 X1091 X1092 X1093 X1094 X1095 X1096 X1097 X1098 X1099                                                           X1100 X1101 X1102 X1103 X1104 X1105 X1106 X1107 X1108 X1109 X1110 X1111 X1112 X1113 X1114 X1115 X1116 X1117 X1118 X1119 X1120 X1121 X1122 X1123 X1124 X1125 X1126 X1127 X1128 X1129 X1130 X1131 X1132 X1133 X1134 X1135 X1136 X1137 X1138 X1139 X1140 X1141 X1142 X1143 X1144 X1145 X1146 X1147 X1148 X1149 X1150 X1151 X1152 X1153 X1154 X1155 X1156 X1157 X1158 X1159                 X1160 X1161 X1162 X1163 X1164 X1165 X1166 X1167 X1168 X1169 X1170 X1171 X1172 X1173 X1174 X1175 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK                                        UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1359 X1360 X1361 X1362 X1363 X1364 X1365 X1366 X1367 X1368 X1369 X1370 X1371 X1372 X1373 X1374 X1375 X1376 X1377 X1378 X1379 X1380 X1381 X1382 X1383 X1384 X1385 X1386 X1387 X1388 X1389 X1390 X1391 X1392 X1393 X1394 X1395 X1396 X1397 X1398 X1399                                                        X1400 X1401 X1402 X1403 X1404 X1405 X1408 X1409 X1410 X1411 X1412 X1413 X1414 X1415 X1416 X1417 X1418 X1419 X1420 X1421 X1422 X1423 X1424 X1425 X1426 X1427 X1428 X1429 X1430 X1431 X1432 X1433 X1434 X1435 X1436 X1437 X1438 X1439 X1440 X1441 X1442 X1443 X1444 X1445 X1446 X1447 X1448 X1449 X1456 X1457 X1458 X1459 X1460 X1461 X1462 X1463 X1464 X1465                                                          X1466 X1467 X1468 X1469 X1470 X1471 X1472 X1473 X1474 X1475 X1476 X1477 X1480 X1481 X1482 X1483 X1484 X1485 X1486 X1487 X1488 X1489 X1490 X1493 X1494 X1495 X1496 X1497 X1498 X1499 X1500 X1501 X1502 X1503 X1504 X1505 X1506 X1507 X1508 X1509 X1510 X1511 X1512 X1513 X1514 X1515 X1516 X1517 X1518 X1519 X1520 X1521 X1522 X1523 X1524 X1525 X1526 X1527                                                  X1528 X1529 X1530 X1531 X1532 X1533 X1536 X1537 X1538 X1539 X1540 X1541 X1542 X1543 X1544 X1547 X1548 X1549 X1550 X1551 X1552 X1553 X1554 X1555 X1556 X1557 X1558 X1559 X1560 X1561 X1562 X1563 X1564 X1565 X1566 X1567 X1568 X1569 X1570 X1571 X1572 X1573 X1574 X1575 X1576 X1577 X1578 X1579 X1580 X1581 X1582 X1583 X1584 X1585 X1586 X1590 X1591                                                X1592 X1593 X1594 X1598 X1599 X1600 X1601 X1602 X1603 X1604 X1605 X1606 X1607 X1608 X1609 X1610 X1611 X1612 X1613 X1614 X1615 X1616 X1622 X1623 X1624 X1625 X1626 X1627 X1628 X1629 X1630 X1631 X1632 X1633 X1634 X1635 X1636 X1637 X1638 X1639 X1640 X1641 X1642 X1643 X1644 X1645 X1646 X1647 X1648 X1649 X1650 X1651 X1652 X1653 X1654 X1655 X1656 X1657                                                      X1658 X1659 X1660 X1661 X1662 X1663 X1664 X1665 X1666 X1667 X1668 X1669 X1670 X1671 X1672 X1673 X1674 X1675 X1676 X1677 X1678 X1679 X1680 X1681 X1682 X1683 X1684 X1685 X1686 X1687 X1688 X1689 X1692 X1693 X1694 X1695 X1696 X1697 X1698 X1699 X1700 X1701 X1702 X1703 X1704 X1705 X1706 X1707 X1708 X1709 X1710 X1711 X1712 X1713 X1714 X1715 X1716 X1717 X1718                                             X1719 X1720 X1721 X1722 X1723 X1724 X1725 X1726 X1727 X1728 X1729 X1730 X1731 X1732 X1735 X1736 X1737 X1738 X1739 X1740 X1741 X1742 X1743 X1744 X1745 X1746 X1747 X1748 X1749 X1750 X1751 X1752 X1753 X1754 X1755 X1756 X1757 X1758 X1759 X1760 X1761 X1762 X1763 X1764 X1765 X1766 X1767 X1768 X1769 Page 24 Full wwPDB EM Validation Report EMD-6695, 5WYJ

• Molecule 13: Utp15

Chain AF:                        X1 X2 X3 X4 X5 X13 X16 X17 X21 X22 X23 X28 X29 X30 X31 X35 X39 X43 X54 X55 X67 X68 X69 X70 X71 X72 X73 X74 X75 X76 X77 X78 X89 X93 X99 X100 X101 X105 X109 X110 X117 X118 X132 X133                     X134 X141 X142 X143 X148 X149 X150 X151 X152 X153 X157 X172 X173 X177 X178 X181 X182 X185 X192 X193 X194 X195 X201 X206 X210 X214 X221 X226 X234 X240 X243 X244 X252 X253 X259 X260 X261 X275 X279 X284 X285 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK    UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X382 X383 X384 X391 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X404 X405 X406 X407    X411 X415 UNK UNK UNK UNK UNK UNK UNK UNK X424 X427 X428 X431 X432 X433 X434 UNK UNK UNK UNK UNK X440 X449 X452 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X468 X474 X478 X481 UNK X483 X495 X505 X509 X513

• Molecule 14: Utp17

Chain AG:                            UNK X2 X3 X4 X7 X8 X9 X10 X11 X12 X13 X14 X15 X30 X31 X32 X33 X36 X44 X45 X48 X53 X56 X57 X58 X59 X63 X79 X88 X95 X96 X97 X98 X99 X100 X104 X108 X111 X112 X113 X114 X115 X116 X117 X118 X119                                X122 X126 X130 X131 X132 X133 X136 X137 X138 X139 X140 X141 X142 X143 X144 X145 X146 X153 X154 X164 X175 X176 X179 X180 X181 X186 X187 X188 X195 X199 X204 X208 X209 X210 X211 X212 X219 X220 X221 X222 X223 X224 X225 X226 X227 X228                                     X229 X230 X233 X237 X238 X239 X243 X244 X250 X251 X260 X261 X262 X263 X264 X271 X274 X279 X280 X281 X282 X283 X284 X285 X292 X293 X294 X295 X296 X297 X298 X299 X300 X301 X302 X303 X304 X305 X306 X307 X308 X312 X313 X314 X315 X316 X317 X318 X319                                   X336 X337 X338 X339 X340 X341 X342 X343 X344 X345 X346 X353 X356 X357 X358 X359 X360 X361 X362 X365 X366 X370 X374 X375 X376 X381 X382 X383 X384 X385 X394 X395 X396 X397 X398 X399 X400 X401 X402 X405 X406 X409 X410 X411 X412 X413 X416 X417 X418 X419                               X420 X421 X422 X423 X424 X438 X441 X442 X443 X444 X445 X446 X447 X448 X449 X450 X451 X452 X453 X454 X457 X458 X459 X460 X464 X465 X469 X470 X475 X483 X484 X485 X486 X487 X488 X489 X495 X496 X497 X502 X508 X509 X510 X513 X525 X526 X527 X528      X536 X537 X538 X543 X546 X547 X548 X549 X567 X568 X569 X570 X571 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK Page 25 Full wwPDB EM Validation Report EMD-6695, 5WYJ UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK    UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X807 X823 X827 X831 X836 X841 X842 X843 X844 X845 X846 X847 X848 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 15: Ribosome biogenesis protein BMS1

Chain B1:       MET GLU GLN SER ASN LYS GLN HIS ARG LYS ALA LYS GLU LYS ASN THR ALA LYS LYS LYS LEU HIS THR GLN GLY HIS ASN ALA LYS ALA PHE ALA VAL ALA ALA PRO GLY LYS MET ALA ARG THR MET GLN ARG SER SER ASP VAL ASN GLU ARG LYS L33 H34 M37 V38 D39 R40                        D44 D45 P46 P47 P48 V53 P57 T63 R66 V69 T73 T76 L77 N78 S88 G89 R93 F96 L97 E98 C99 P100 A101 D102 D103 A106 D109 I110 A111 K112 I113 A114 D115 L116 V117 L118 L119 L120 F125 G126 F127 E128 M129 E133 F134                     I137 H141 G142 M143 P144 R145 V146 L147 G148 V149 A150 T151 L155 Q159 W174 A181 K182 L183 F184 Y185 L186 S187 G188 V189 I190 R193 Y194 P195 D196 R197 L200 N201 R204 F205 I206 S207 K210 F211 R212 P213 L214 K215 W216 E219 H220 P221 Y222 A225                D226 D230 L231 T232 H233 P234 E235 L236 I237 E238 T239 Q243 I244 D245 R246 A249 G252 Y253 G256 L259 P260 R266 V267 H268 I269 A270 D274 F275 A278 E281 K282 L283 P284 ASP PRO CYS PRO THR PRO PHE TYR GLN GLN LYS LEU ASP ASP PHE GLU ARG GLU LYS MET LYS GLU GLU ALA LYS ALA ASN GLY GLU ILE THR THR ALA SER THR THR ARG ARG ARG LYS ARG LEU ASP ASP LYS ASP LYS LEU ILE TYR ALA PRO MET SER ASP VAL GLY GLY VAL LEU MET ASP LYS ASP ALA VAL TYR ILE ASP ILE GLY LYS LYS ASN GLU GLU PRO SER PHE VAL PRO GLY GLN GLU ARG GLY GLU GLY GLU LYS LEU MET THR GLY LEU GLN SER VAL GLU GLN SER ILE ALA GLU LYS PHE ASP GLY VAL GLY LEU GLN LEU PHE SER ASN GLY THR GLU LEU HIS GLU VAL ALA ASP HIS GLU GLY MET ASP VAL GLU SER GLY GLU GLU SER ILE GLU ASP ASP GLU GLY LYS SER LYS GLY ARG THR SER LEU ARG LYS PRO ARG ILE TYR GLY LYS PRO VAL GLN GLU GLU ASP ALA ASP ILE ASP ASN LEU PRO SER ASP GLU GLU PRO TYR THR ASN ASP ASP ASP VAL GLN ASP SER GLU PRO ARG MET VAL GLU ILE ASP PHE ASN     ASN THR GLY GLU GLN GLY ALA GLU LYS LEU ALA LEU GLU THR ASP SER GLU PHE GLU GLU SER GLU ASP GLU PHE SER TRP GLU ARG THR ALA ALA ASN LYS LEU LYS LYS THR GLU SER LYS LYS ARG THR W547 N548 K551 D556 N557 I558 S559 P560 G569 GLU ASP ASP ASP        SER LYS ASP GLU SER ASP ILE GLU GLU ASP VAL ASP ASP ASP PHE PHE ARG LYS LYS ASP GLY THR VAL THR LYS GLU GLY ASN LYS ASP HIS ALA V606 D607 L608 E609 K610 F611 V612 D616 K623 K624 W625 K626 A630 R634 F635 L636 GLY ALA GLY ILE LEU GLY ASN ASP ASN LYS THR LYS SER ASP SER ASN GLU GLY GLY GLU GLU LEU TYR GLY ASP PHE GLU ASP LEU GLU ASP GLY ASN PRO SER GLU GLN ALA GLU ASP ASN SER ASP LYS GLU SER GLU ASP GLU ASP GLU ASN GLU ASP THR ASN GLY ASP ASP ASP ASN SER PHE THR ASN PHE ASP ALA Page 26 Full wwPDB EM Validation Report EMD-6695, 5WYJ GLU GLU LYS LYS ASP LEU THR MET GLU GLN GLU ARG GLU MET ASN ALA ALA LYS LYS GLU LYS LEU ARG ALA GLN PHE GLU ILE GLU GLU GLY GLU ASN PHE LYS GLU ASP ASP GLU ASN ASN GLU TYR ASP THR TRP TYR GLU LEU GLN LYS ALA LYS ILE SER LYS GLN LEU GLU ILE            ASN ASN ILE GLU TYR GLN GLU MET THR PRO GLU GLN ARG GLN ARG ILE GLU GLY PHE LYS ALA GLY S787 Y788 V789 R790 I791 V792 P797 P809 M812 G813 G814 L815 L816 P817 T818 E819 F822 G823 I824 A827 R828 L829 R830 R831 H832 R833 W834 H835 K836 K837 I838 L839 K840                        T841 N842 L845 V846 L847 R852 R853 F854 Q855 T856 L857 T863 D864 R868 T869 Y874 T875 P876 C881 Y886 G887 P888 L889 C890 S891 P892 N893 T894 P895 V899 Q900 I901 S905 D906 T907 G908 N909 G910 F911 R912 I913 I918 V919 E920 E921 I922 D923 V924 I928                             K931 L932 K933 L934 V935 G936 F937 P938 Y939 K940 I941 F942 K943 N944 T945 A946 F947 I948 M951 E957 R960 F961 E962 Q965 I966 K967 T968 V969 S970 G971 I972 R973 G974 E975 I976 K977 R978 A979 L980 S981 K982 P983 E984 R988 A989 E992 D993 K994 I995 L996 M997 S998 D999 I1000 R1004 S1005 W1006 K1012 N1016 PRO VAL THR SER LEU LEU LEU LYS GLU LYS THR GLU TRP LYS GLY LEU ARG LEU THR GLY GLN ILE ARG ALA ALA MET ASN LEU GLU THR PRO SER ASN PRO ASP SER ALA TYR HIS LYS ILE GLU ARG VAL GLU ARG HIS PHE ASN GLY LEU LYS VAL PRO LYS ALA VAL GLN LYS GLU LEU PRO PHE LYS SER GLN ILE HIS GLN MET LYS PRO GLN LYS LYS LYS THR TYR MET ALA LYS ARG ALA VAL VAL LEU GLY GLY ASP GLU LYS LYS ALA ARG SER PHE ILE GLN LYS VAL LEU THR ILE SER LYS ALA LYS ASP SER LYS ARG LYS GLU GLN LYS ALA SER GLN ARG LYS GLU ARG LEU LYS LYS LEU ALA LYS MET GLU GLU GLU LYS SER GLN ARG ASP LYS GLU LYS LYS LYS GLU TYR PHE ALA GLN ASN GLY LYS ARG THR THR MET GLY GLY ASP ASP GLU SER ARG PRO ARG LYS MET ARG ARG

• Molecule 16: Periodic tryptophan protein 2

Chain BA:                        M1 K2 S3 D4 F5 S8 N9 L10 L11 G12 T13 V14 Y15 R16 Q17 T21 D24 D25 Q28 L29 L30 S31 P32 V33 G34 N35 R36 V37 S38 V39 F40 D41 N45 F48 T49 F50 E51 Y52 E53 N57 I58 A59 A60 I61 D62 L63 N64 K65 Q66 G67 T68 S72                I73 D74 E75 D76 G77 R78 L81 V82 K85 A86 R87 N88 V89 L90 H91 H92 F93 N94 F95 K96 E97 K98 C99 K103 F111 A112 L113 A114 S115 G116 R117 F118 L119 W122 D126 V127 N128 K129 D130 R131 Q132 F133 A134 P135 R138 H139 H142 H145 D148 I149                   T150 S151 L152 T153 W154 S155 Q156 R159 F160 I161 L162 T163 T164 S165 K166 D167 L168 S169 A170 K171 I172 W173 S174 V175 D176 A183 A184 T185 T186 F187 N188 G189 H190 R191 D192 Y193 V194 M195 G196 A197 F198 F199 S200 H201 I206 Y207 K211 D212 V217 K222 ARG PRO SER ASP                 ASP ASP ASP ASN GLU SER GLU ASP ASP ASP LYS GLN GLU GLU VAL ASP ILE SER K245 Y246 S247 W248 R249 A258 V263 K264 C265 V266 T267 F268 F279 E283 F284 L289 M300 V305 N306 T307 V308 S309 V310 W316 F319 G320 S321 S322 K323 L324 L328 V329                             L339 Q342 G343 H344 F345 D346 S347 T348 N349 P355 D356 R359 T362 A363 D366 G367 K368 G377 F378 C379 L380 E384 E385 A392 V393 Q394 F395 A396 K397 R398 G399 Q400 S404 S405 G409 R412 R418 Y419 R420 N421 F422 R423 T426 G427 T428 E429                           R430 I431 Q432 F433 N434 V438 D439 P440 S441 V445 C446 S449 L450 D451 N452 F453 H456 V457 W458 Q461 L465 L466 D467 A468 G471 H472 E473 G474 P475 V476 S477 N485 S486 S490 A491 S492 W493 D494 K495 T496 I497 R498 I499 W500 F503 G504 R505 S506 Q507 Q508                 V509 E510 V514 Y515 S516 D517 V518 L519 A520 L521 S522 M523 G527 V532 S533 T534 L535 K536 G537 Q538 I539 S540 I541 F542 D546 A547 K548 Q549 V550 G551 N552 I553 D554 C555 R556 K557 D558 I559 ILE SER GLY ARG PHE ASN GLN ASP ARG PHE THR ALA LYS ASN SER GLU ARG S577 Page 27 Full wwPDB EM Validation Report EMD-6695, 5WYJ            K578 F579 F580 T581 T582 I583 H584 Y585 S586 F587 I592 V593 A594 G595 G596 N597 N598 N599 S600 I601 C602 L603 Y604 D605 N608 L611 L612 F615 S618 R619 N620 MET ALA LEU ASN GLY THR LEU GLU PHE LEU ASN SER LYS LYS MET THR GLU ALA GLY SER LEU ASP LEU ILE              ASP ASP ALA GLY GLU ASN SER ASP LEU GLU ASP ARG ILE ASP ASN SER LEU PRO GLY SER GLN ARG GLY GLY ASP LEU SER THR ARG LYS MET ARG PRO GLU V679 R680 V681 T682 S683 V684 Q685 F686 T689 A694 A695 T698 E699 L702 I703 T706 N707 D708 T709 I710 L711             F712 D713 P714 F715 D716 L717 D718 V719 D720 V721 R731 F743 R744 L745 N746 I751 N752 Y755 E756 I770 R777 K780 F781 I782 G783 D784 E788 I792 N795 L796 I797 W798 I799 L803 N811 Y815 L816 F817 M821 R822 Q825 R826 F827 I828 V829   R830 N837 N842 Y846 R847 T852 ASP GLY SER MET GLU ASP GLY ALA ALA ASP ASP ASP GLU VAL LEU LEU LYS ASP ASP ALA ASP GLU ASP ASN GLU GLU ASN GLU GLU ASN ASP VAL VAL MET GLU SER ASP ASP GLU GLU GLY TRP ILE GLY PHE ASN GLY LYS ASP ASN LYS LEU PRO LEU SER ASN GLU ASN ASP SER SER ASP GLU GLU GLU ASN GLU LYS GLU LEU PRO

• Molecule 17: U3 small nucleolar RNA-associated protein 12

Chain BB:                                MET VAL LYS SER TYR GLN ARG PHE GLU GLN A11 A12 G15 V16 I17 A18 S19 N20 A21 N22 C23 V24 W25 I26 S30 G31 N34 G35 S36 G37 P38 L41 S44 A45 L46 E47 D48 V49 N50 I51 W52 K55 L59 V60 L63 S64 D65 G66 L67 P68 P69 G70                        A71 S72 D73 A74 R75 A80 E86 A87 H88 K89 D90 A95 V96 A99 I103 F117 N118 K121 A122 A123 I124 T125 L126 G131 R135 S138 G139 S140 K141 D142 W148 D149 L150 V151 G152 E153 V154 K158 K163 D164 S165 I166 T167 G168 F169 W170 C171              Q172 G173 E174 S179 T180 S181 K182 M185 I186 K187 L188 E198 T199 H200 I201 T204 G209 L210 A211 V212 K213 D214 D215 L216 L217 I218 T219 T220 Q225 V226 K227 I228 W229 K230 L231 N235 D236 K237 M238 G239 G240 K241 L242 T243 E244 M245 E249 K250 Q251 S252 K253 Q254      R255 G256 L257 K258 F261 D267 F271 F272 Q275 ASN ALA ASP LYS THR ILE GLU THR PHE ARG ILE ARG LYS GLU GLU GLU ILE ALA ARG GLY LEU LYS LYS ARG GLU LYS ARG LEU LYS GLU LYS GLY LEU THR GLU GLU GLU ILE ALA LYS SER ILE LYS GLU SER Y321                                  L326 H327 P328 F329 Q330 T331 I332 R333 S334 L335 A341 S342 W343 T344 T345 V346 S347 S348 S349 K350 L351 T357 S358 S359 N360 T361 I367 P368 R372 D373 P374 K381 T382 H383 T384 I385 E386 L387 Q390 R391 T392 D393 V394 I397 D398 I399 S400 D401 D402 N403 K404 L405 L406                    A407 T408 A409 S410 N411 L414 W417 K423 R426 T427 F428 E429 C430 L434 K437 F438 L439 P440 G441 G442 L443 L444 V445 I446 L447 L454 Q455 L456 F457 D458 L459 S462 S463 L464 L465 D466 T467 I468 E469 D473 S478 L479 D480 L481 T482 S483 D484 L488                            G491 T496 F499 W500 D501 F502 N506 S507 L508 V509 P510 K513 N514 K515 F516 L517 P518 V519 L520 H524 D525 T526 T527 L528 E529 L530 T531 I534 L535 C536 V537 R538 V539 D542 D543 R544 Y545 S549 L550 L551 D552 N553 T554 V555 F559 L560 D561 S562 M563 K564 F565               S568 L569 Y570 G571 H572 K573 L574 P575 V576 I579 K586 M587 S592 A593 D594 K595 N596 I597 K598 D603 L611 Q615 D616 S617 I618 M619 N620 V621 K622 F623 L624 P625 H628 N629 F630 F631 S634 K635 D636 A637 V638 V639 K640 Y641 W642 C649 I650 Q651 K652 L653 Page 28 Full wwPDB EM Validation Report EMD-6695, 5WYJ      H656 A662 L663 F671 S674 D678 H679 S680 I681 R682 E685 GLU THR GLU ASP GLN VAL PHE LEU GLU GLU GLU LYS GLU LYS GLU LEU GLU GLU GLN TYR GLU ASP THR LEU LEU THR SER LEU GLU GLU GLY ASN GLY ASP ASP ALA PHE LYS ALA ASP ALA SER GLY         GLU GLY VAL GLU ASP GLU ALA SER GLY VAL HIS LYS GLN THR LEU GLU SER LEU K747 A748 G749 E750 R751 L752 D757 E764 G765 N770 R771 D772 M773 LYS LEU TRP GLN ARG LYS LYS LEU GLY GLU ALA PRO ILE LYS PRO GLN GLY ASN ALA V793 L794 I795 A796 V797 N798 K799                T800 R811 I812 D819 A820 L821 M822 V823 Y828 F832 L833 I836 D837 T838 N842 L846 H847 P851 K855 N856 L857 I860 F863 N864 H865 K866 E867 Q871 K877 R882 V883 K884 R888 K892 L898 G899 F900 Q903 G904 L905 K906 F907 N913 LEU ARG HIS ASN TYR GLU PHE VAL ASP GLU TYR ASP GLN GLN GLU LYS GLU SER ASN SER ALA ARG LYS ARG VAL PHE GLY THR VAL ILE

• Molecule 18: U3 small nucleolar RNA-associated protein 13

Chain BC:                           MET ASP LEU LYS THR SER TYR LYS GLY ILE SER LEU N13 P14 I15 Y16 A17 G18 A21 V22 A23 T24 V25 S26 E27 N28 G29 K30 A33 T34 P35 V36 L37 D38 E39 I40 N41 I42 I43 D44 L45 T46 P47 G48 S49 R50 K51 I52 L53 H54 K55 I56 S57 N58 Q62 E63 I64                                  T65 A66 L67 K68 L69 D72 G73 Q74 Y75 L76 T77 Y78 V79 S80 Q81 A82 Q83 L84 L90 K91 T92 G93 K94 R97 S103 P104 S105 Y106 I107 L108 D109 A110 D111 T115 L116 L117 A118 V119 G120 G121 T122 D123 G124 S125 I126 I127 E132 N133 G134 Y135 I136 T137 H138 S139                                        F140 K141 I147 S148 S149 L150 G154 N157 S158 K159 I160 A164 S165 M171 V172 K173 V174 R180 K181 C182 L183 H184 T185 L186 Q187 T190 S191 A192 V193 L196 D197 I198 I199 E200 V201 P202 D203 N204 D205 E206 P207 S208 L209 N210 L211 L212 S213 G214 G215 R216 I220                               N221 L222 W223 N226 M227 K228 K229 K230 C231 K232 K235 T236 L237 P238 V239 N240 Q241 Q242 V243 E244 S245 C246 K250 D251 G252 D253 G254 K255 R256 I257 I258 Y259 T260 A261 G262 G263 D264 A265 I266 F267 Q268 L269 I270 D271 S272 E273 S274 G275 S276 V277 L278 K279 R280 T281 N282 K283 P284                                      I285 E286 E287 I290 I291 G292 L297 S298 N299 S300 Q301 M302 F303 L304 S307 D308 Q309 T310 L311 I314 N315 V316 E317 E318 D319 L320 K321 N322 D323 E324 D325 T326 I327 Q328 V329 T330 S331 S332 I333 A334 G335 N336 I339 I340 M343 R344 Y345 V346 G347 P348 E349 L350 N351 K352                                     L353 A354 L355 A356 T357 N358 S359 L362 R363 I364 I365 P366 V367 L370 E374 A375 S376 L377 P378 L379 D380 V381 E382 I383 Y384 E385 G386 H387 L391 N392 S393 L394 D395 A396 T397 E398 D399 G400 A404 S407 A412 N420 S421 C422 K423 F424 D425 I426 Y427 A428 K429 Y430                                         S434 T438 A439 V440 P443 N444 I445 V446 S447 Y450 P451 L455 T456 A457 T462 I463 K464 K465 W466 I467 I468 P469 K470 P471 T472 A473 S474 M475 D476 V477 Q478 I479 I480 K481 V482 S483 E484 R487 H488 A489 H490 E491 K492 D493 I494 L497 S498 V499 D503 S504                                     T508 A509 S510 Y511 D512 C515 N519 L520 E521 N522 G523 E524 L525 E526 A527 T528 L529 A530 N531 H532 K533 L536 W537 D538 F541 C542 Q543 Y544 D545 K546 L547 L548 A549 T550 G553 D554 K555 I559 L562 F565 S566 V567 M568 K569 T570 G573 H574 T575 N576 R580                         C581 S582 F583 I584 N585 K586 Q587 K588 Q589 S592 D596 G597 L598 I599 D603 C604 S605 S606 G607 E608 C609 L610 L613 D614 G615 H616 L620 W621 A622 L623 D628 A635 D636 A637 D638 F641 Q642 F643 W644 LYS ASP CYS THR GLU GLN GLU ILE GLU GLU GLU GLN GLU Page 29 Full wwPDB EM Validation Report EMD-6695, 5WYJ                       LYS ALA K660 L661 Q662 E666 Q667 N680 A681 L684 A685 M686 T687 L688 D689 H690 P691 V697 L698 K699 R700 A701 L702 G703 R706 S707 R708 Q709 D710 T711 E712 E713 G714 K715 I716 E717 V718 I719 F720 N721 E722 D725 I728 S729 I730 R742 N747 T748 K751 T752       H753 T759 I760 R761 C762 I763 L764 K771 L772 S773 E774 I775 K780 D783 T789 I805 L806 L810 VAL GLU MET ASP LYS LEU PHE

• Molecule 19: U3 small nucleolar RNA-associated protein 18

Chain BD: MET THR MET ALA THR THR ALA MET ASN VAL SER VAL PRO PRO PRO ASP GLU GLU GLU GLN LEU LEU ALA LYS PHE VAL PHE GLY ASP THR THR ASP LEU GLN GLU ASN LEU ALA LYS PHE ASN ALA ASP PHE ILE PHE ASN GLU GLN GLU MET ASP VAL GLU ASP GLN GLU ASP GLU GLY SER GLU SER ASP ASN SER GLU GLU ASP GLU ALA GLN ASN GLY GLU LEU ASP HIS VAL ASN ASN ASP GLN LEU PHE PHE VAL ASP ASP GLY GLY ASN GLU ASP SER GLN ASP LYS ASN GLU ASP THR MET ASP VAL ASP ASP GLU ASP ASP SER SER SER ASP ASP TYR SER GLU ASP SER GLU GLU ALA ALA TRP ILE ASP SER ASP ASP GLU LYS ILE LYS VAL PRO ILE LEU VAL THR ASN LYS THR LYS LYS LEU ARG THR SER TYR ASN GLU SER LYS ILE ASN GLY VAL HIS TYR ILE ASN ARG LEU ARG SER GLN PHE GLU LYS ILE TYR PRO ARG PRO LYS TRP VAL ASP ASP   GLU SER ASP SER GLU LEU ASP ASP GLU GLU ASP ASP GLU GLU GLU GLY SER ASN ASN VAL ILE ASN GLY ASP ILE ASN ALA LEU THR LYS ILE LEU SER THR THR TYR ASN TYR LYS ASP THR LEU SER ASN SER LYS LEU LEU PRO PRO LYS LYS LEU ASP I235 V236 R237 L238 K239 D240                                     H245 P246 S247 H248 S249 A250 I251 S259 K260 P261 L262 D269 L272 R273 I274 Y275 D278 G279 K280 T281 N282 H283 L284 V285 T286 S287 L288 H289 L290 V291 G292 Q296 T297 C298 T299 F300 Y301 T302 S303 L304 S305 N306 Q307 N308 Q309 Q310 N311 I312 F313 T314 R317 R318 R319                 Y320 M321 H322 S323 W324 D325 L326 S327 LEU GLU ASN LEU THR HIS SER Q335 T336 A337 K338 I339 F342 S343 R344 L345 Y346 G347 H348 E349 S350 T351 Q352 R353 S354 F355 E356 N357 H362 L363 Q364 Q367 T368 N369 S370 V371 H372 GLY ILE VAL LEU LEU GLN GLY ASN ASN G382 W383 I384                            N385 I386 L387 H388 S389 T390 S391 G392 L395 M396 G397 C398 K399 V403 I404 T405 D406 F407 C408 I409 D410 Y411 Q412 P413 I414 S415 R416 G417 K418 F419 I422 L423 I424 A425 V426 N427 A428 Y429 G430 E431 V432 W433 E434 F435 D436 L437 N438 K439 N440 G441 H442 V443 I444 R445 R446 W447 K448                       G452 V453 G454 I455 I458 Q459 G462 GLY THR THR THR THR CYS PRO ALA LEU GLN ILE SER LYS ILE LYS GLN N479 R480 W481 L482 A483 S486 E487 S488 G489 F490 Y494 D495 R496 N497 N498 A499 M500 T501 V508 D512 Q513 T516 T517 I518 S519 N520 L521 Q522 F523                  S524 I529 L530 C531 M532 A533 S534 V537 K538 D539 A540 L541 R542 L543 V544 C549 S550 V551 F552 S553 N554 W555 P556 G559 T560 P561 L562 G563 K564 V568 S573 G574 G575 L576 L577 A578 N581 K585 L588 W589 K590 L591 ASN HIS TYR

• Molecule 20: U3 small nucleolar RNA-associated protein 21

Chain BE:             MET SER ILE ASP LEU LYS LYS ARG LYS VAL GLU GLU ASP VAL ARG SER ARG GLY LYS N20 S21 K22 I23 F24 F27 N32 V33 S34 N35 G36 V37 P38 F39 A40 V51 T52 C53 F58 Q59 I60 Y61 D62 A63 N64 T65 L66 H67 F70 E73 T76 P77 S78 S79 Page 30 Full wwPDB EM Validation Report EMD-6695, 5WYJ                       I80 V81 A82 L83 S84 Y91 A92 A93 Y94 E95 N96 K97 V98 E107 H108 L109 L110 E111 L112 E113 T114 D115 A116 N117 V118 L121 C122 I123 F124 L128 S131 T132 D133 D134 N135 S136 I137 F138 I139 Y140 K141 K142 S143 D144 P145 Q146 D147 F153 Y154 T155 K156 L157 T158 V159                          Q163 G164 L170 Q171 H172 L173 A174 T175 Y176 K179 V188 L189 L190 F191 N192 V193 R194 T195 G196 V199 F200 F205 Q208 I209 A212 E213 P214 A215 P216 V217 L218 A222 L223 V226 E229 V230 I231 M232 G238 R242 T243 I244 K245 I246 P247 Q248 S249                    R250 L254 R257 T258 D259 H263 V266 D272 L273 I274 F275 R280 R281 S282 R283 I284 H285 V286 L287 K288 N289 I290 H291 R292 E293 S294 Y295 G296 G297 V298 T299 Q300 P308 I309 I310 V311 G315 D316 N317 S318 L319 K320 E321 Y322 V323 F324 D325 P326 SER LEU SER GLN                 GLY SER GLY ASP VAL VAL VAL Q338 P339 P340 R341 Y342 L343 R344 S345 R346 H349 S350 P353 S354 Y355 I356 S363 H364 F365 K371 D372 R373 S374 L375 W376 S377 F378 S379 L380 R381 K382 D383 A384 Q385 S386 M389 S390 Q391 R392 LEU HIS LYS LYS GLN ASP GLY GLY ARG VAL                        GLY GLY SER THR ILE LYS S409 P412 E413 I414 V415 A416 L417 A418 I419 E420 N421 A422 R423 I424 G425 E426 N429 I430 I431 H434 K435 D436 E437 K438 F439 A440 R441 T442 W443 D444 M445 K448 R449 V450 G451 R452 F455 D456 T457 T458 D459 D460 G461 F462 V463 K464 S465 V466 A467                         M468 S469 Q470 C471 G472 N473 F474 G475 F476 I477 S480 N481 I484 T485 I486 Y487 N488 M489 K497 Y498 K499 R503 A504 V505 T506 G507 I508 S509 L510 D511 G512 M513 N514 R515 C520 G521 L522 I525 V526 Y529 D530 F531 S534 T535 K539 L540 K541 L542 D543 A544 P545           I546 T547 A548 Y551 D556 L557 F558 A559 L560 A561 L562 D563 S566 I567 V568 V569 A572 V573 T574 Q575 R576 V577 V578 R579 Q580 L581 W582 G583 R587 I588 D592 F593 S594 P595 E596 G597 R598 V601 S602 A603 S604 L605 I609 R610 T611 W612 D613 T616 G617 G618 C619           I620 D621 G622 I623 D626 N627 V628 A629 T630 N631 V632 K633 F634 G638 D639 L640 L641 A642 T643 T644 T647 G648 N649 G650 I651 C652 I653 W654 T655 Q659 F660 T665 R666 T667 S671 GLU PHE ALA ARG MET ALA LEU PRO SER THR SER VAL ARG GLY ASN ASP SER MET LEU SER GLY ALA LEU GLU SER ASN GLY GLY GLU ASP LEU ASN ASP ILE ASP PHE ASN THR TYR THR SER LEU GLU GLN ILE ASP LYS GLU LEU LEU THR LEU SER ILE GLY PRO ARG SER LYS MET ASN THR LEU LEU HIS LEU ASP VAL ILE ARG LYS ARG SER LYS PRO LYS GLU ALA PRO  LYS LYS SER GLU LYS LEU PRO PHE PHE LEU GLN LEU SER GLY GLU LYS VAL GLY ASP GLU ALA SER VAL ARG GLU GLY ILE ALA HIS GLU THR PRO GLU GLU ILE HIS ARG ARG ASP GLN GLU ALA Q793 K794 K795 L796 D797 E800 F805 LYS VAL THR GLY ARG LEU GLY PHE GLU              SER H816 K828 I838 N839 F840 I849 ARG SER LEU ASN SER PHE GLU PRO PHE D859 E860 I861 V862 W863 F864 I865 L868 T869 Q870 E882 H892 I896 N900 Q903 D904 I905 A906 S907 A908 L909 Q910 E913 D914 K918 R921 M930 G931 V932 A933 T937 T938 A939

• Molecule 21: Ribosomal RNA-processing protein 7

Chain CA:                                   MET GLY I3 E4 D5 I6 S7 K10 N11 G12 F13 I14 V15 V16 P17 F18 L26 P27 LYS SER GLN GLU A32 S33 L34 H35 F36 M37 F38 A39 K40 R41 H42 Q43 S44 S45 N46 S47 N48 E49 S50 D51 C52 L53 F54 L55 V56 N57 L60 L61 E65 F70 L74 C75 Page 31 Full wwPDB EM Validation Report EMD-6695, 5WYJ                              G76 K77 V81 S82 H83 V84 E85 E86 L87 L88 Y89 N90 D91 E92 F93 G94 L95 H96 E97 V98 D99 L100 S101 A102 L103 T104 S105 ASP LEU MET SER SER THR ASP VAL ASN GLU LYS ARG TYR THR P120 R121 N122 T123 A124 L125 L126 V129 D130 A131 A132 S133 I134 C137 W138                                K142 K143 Y144 S145 N146 L147 H148 A149 K150 H151 P152 N153 E154 L155 F156 E157 W158 T159 Y160 T161 T162 P163 S164 F165 T166 T167 F168 V169 N170 F171 Y172 K173 P174 L175 D176 I177 L180 K181 E182 D183 I184 H185 T186 H187 M188 A189 I190 F191 E192 E195 A196 Q197 A198 Q199 Q203 S204  D208 G211 L214 V215 V216 GLY LYS ASN THR LYS SER LEU ASN SER ILE ARG LYS LYS ILE LEU ASN LYS ASN PRO LEU SER LYS HIS GLU ASN LYS ALA LYS PRO ILE SER ASN ILE ASP LYS LYS ALA LYS LYS ASP PHE TYR ARG PHE GLN VAL ARG GLU ARG LYS LYS GLN GLU ILE ASN GLN LEU LEU SER LYS PHE LYS GLU ASP GLN GLU ARG ILE LYS VAL MET LYS ALA LYS ARG LYS PHE ASN PRO TYR THR

• Molecule 22: U3 small nucleolar RNA-associated protein 22

Chain CB: MET ALA THR SER VAL LYS ARG LYS ALA SER GLU THR SER ASP GLN ASN ILE VAL LYS VAL GLN LYS LYS HIS SER THR GLN ASP SER THR THR ASP ASN GLY SER LYS GLU ASN ASP HIS SER SER GLN ALA ILE ASN GLU ARG THR VAL PRO GLU GLN GLU ASN ASP GLU SER ASP THR                               SER PRO GLU SER ASN GLU VAL ALA THR ASN THR ALA ALA THR ARG HIS ASN GLY LYS VAL T81 A82 T83 E84 S85 Y86 D87 I88 H89 I90 A91 R92 E93 T94 A95 E96 L97 F98 K99 S100 F103 K104 I107 L111 K115 L116 K117 H120 V121 L122 E125 K126 F127 L128                                           H129 K130 L131 Y132 D133 I134 L135 Q136 E137 I138 P139 D140 W141 E142 E143 K144 S145 L146 A147 E148 V149 D150 S151 F152 F153 K154 N155 K156 I157 V158 S159 F162 V163 D164 P165 K166 P167 I168 P169 Q170 N171 T172 N173 Y174 K175 F176 N177 Y178 K179 K180 P181 D182 I183 S184 L185 I186 G187 S188                                 A193 G194 I195 N199 G200 S201 S202 I203 D204 L207 T208 M209 P210 K211 E212 L213 F214 E215 K216 K217 D218 F219 F222 R223 C224 L225 H226 K227 R228 Y231 L232 L235 T236 H237 H238 L239 L242 L243 K244 K245 D246 K247 L248 D249 Q253 L254 F259 D260 N261 D262 P266                                   I267 L268 R269 I270 S271 C272 S273 K274 PRO THR GLY ASP SER LEU SER D282 Y283 R289 F290 S291 I292 N293 L294 L295 I296 G297 F298 P299 Y300 K301 E304 P305 K306 K307 L308 L309 P310 N311 R312 N313 C314 I315 R316 I317 ALA GLN GLU SER LYS GLU GLN SER L326 P327 A328 T329 P330 L331                                     Y332 N333 F334 L337 S338 S339 S340 N344 Y345 L346 K347 Y348 L349 T352 K353 K354 Q355 S358 F359 A362 T363 V364 L365 G366 R367 Q371 G374 F375 S376 S377 N378 H381 S382 G383 S384 L385 G386 G387 F388 F391 L396 M397 A398 A399 L400 L401 G404 G405 I406                                N407 S408 N409 K410 I411 L412 L413 H414 Y419 Q420 L421 F422 K423 G424 V425 I426 K427 Y428 T431 M432 D433 H436 D437 F442 H443 S444 N445 PRO GLU ASN SER SER SER SER P453 D459 Q463 T464 T472 T478 K479 M480 I487 L488 Y491 A492 G493 E494 T495 L496                             R497 M498 L499 N500 N501 V502 V503 Q504 D505 Q506 F507 S508 N509 I510 F511 L512 T513 N514 F518 D519 N520 L521 K522 Y523 D524 L525 C526 Y527 Q530 L531 P532 L533 Y536 N537 N538 L539 E540 T541 S542 L543 A544 A545 T546 F547 G548 S549 M550 E551 R552 V553 K554 F555 I556 T557 L558 E559                                    L562 A563 H564 K565 I566 T567 A570 R571 Y572 A573 L574 G575 D576 R577 I578 Q582 I583 Q588 K589 S590 D591 F592 R597 K598 V599 N602 H607 F608 N609 F610 D611 R614 V615 K616 L617 I618 V619 N620 P621 C624 D625 K626 L627 V628 T629 K630 G631 P632 S635 E636 T637 Page 32 Full wwPDB EM Validation Report EMD-6695, 5WYJ                                 M638 S639 A643 V644 N647 F648 W649 K652 S653 S654 L655 R656 R657 F658 K659 D660 G661 C666 C667 V668 W669 S670 T671 S672 S673 S674 E675 S679 N683 L686 H689 V690 S691 K692 K693 A694 Q695 I696 S697 N698 E699 F704 F707 L708 P709 N712 S715 S716                                     A717 K718 T719 S720 V721 L722 N723 Y738 F742 K745 L746 P747 L748 K751 S752 I753 L754 P755 V756 G757 S758 R761 Y762 T763 S764 L765 C766 Q767 P768 A772 Y773 S774 D775 P776 D777 F778 F779 Q780 D781 V782 I783 L784 E785 F786 E787 T788 S789 P790 K791 W792 P793 D794 E795 I796                             T797 S798 L799 E800 A805 F806 L807 L808 K809 I810 Q811 E812 E813 L814 S815 A816 N817 S818 S823 S826 R827 D828 E829 S830 I831 N834 L835 E836 I837 V838 T839 L840 N841 I842 E846 G847 Y848 G849 F850 K851 F852 R853 V854 L855 T856 E857 R858 D859 E860 I861 L862 Y863 L864 R865 A866                             I867 A868 N869 A870 E873 E877 L878 E879 A880 L883 K884 A887 K888 Y889 H895 T896 R897 T898 L899 E900 S903 H904 Q907 F908 Y909 V913 R914 L915 R918 D921 T922 H923 L924 L925 L926 G927 H928 I929 T930 D931 I937 A938 I939 K940 P941 F942 V943 A946                         P947 Y948 F949 I950 P951 G952 S953 L954 E955 K960 V961 L962 N969 W970 K971 D972 L975 I976 L977 D978 L979 E983 ASP ASP ILE ARG ASP THR PHE GLU THR SER ILE GLY ALA GLY SER GLU LEU ASP SER LYS THR MET LYS LYS LEU SER E1010 R1011 L1012 Y1017 I1020 Q1021                                   L1027 R1028 P1032 N1033 G1034 T1035 H1036 L1037 Q1038 F1039 F1040 V1041 K1044 N1045 D1046 P1047 L1051 Y1052 S1053 S1054 G1055 I1056 P1059 I1060 R1063 L1064 T1065 A1066 L1067 A1068 K1069 V1070 L1074 H1078 Q1082 F1089 T1090 P1091 G1092 L1093 K1094 D1095 Y1096 D1097 F1098 V1099 V1100 R1103 T1104                            P1105 I1106 G1107 L1108 C1112 L1115 S1116 ALA THR GLU PHE LYS ASN ILE THR ASN ASP GLN A1128 P1129 S1130 N1131 F1132 L1136 N1137 D1138 L1139 S1140 E1141 K1142 M1143 D1144 P1145 T1146 Y1147 Q1148 L1149 V1150 K1151 Y1152 L1153 Y1157 K1158 N1159 S1160 L1161 I1162 L1163 S1164 S1165 R1166 K1167 Y1168 I1169 N1172                                    E1175 K1179 N1180 V1181 I1182 T1183 I1186 L1189 F1190 H1194 K1195 F1196 R1197 V1198 N1199 L1200 D1201 C1202 N1203 V1204 K1205 P1206 D1209 E1210 N1211 V1212 E1222 I1223 A1224 A1225 F1226 G1227 N1228 D1229 M1230 V1231 I1232 N1233 F1234 E1235 T1236 D1237

• Molecule 23: Ribosomal RNA small subunit methyltransferase NEP1

Chain E1:                  MET VAL GLU ASP SER ARG VAL ARG ASP ALA LEU LYS GLY GLY ASP GLN LYS ALA LEU PRO ALA SER LEU VAL PRO GLN ALA P28 P29 V30 L31 T32 S33 K34 D35 K36 K39 R40 M41 I42 V43 V44 L45 A46 M47 A48 S49 L50 E51 T52 H53 K54 I55 SER SER ASN GLY PRO GLY                      GLY ASP K64 Y65 V66 L67 L68 L76 L77 M80 D83 I84 S85 E86 A87 R88 P89 D90 I91 Q94 L97 T98 L99 L100 D101 S102 P103 I104 N105 K106 A107 L110 Q111 V112 Y113 I114 Q115 T116 G119 I120 L121 I122 E123 V124 N125 P126 T127 V128 R129 F134 L140                                 L144 K147 L148 S149 I150 R151 S152 V153 N154 S155 E156 E157 K158 K161 V162 I163 L171 P172 K177 V178 T179 L180 S181 F182 D183 R188 V189 Q190 D191 Y192 K195 L196 D197 D198 D199 I202 C203 A208 M209 A210 R211 G212 K213 D214 N215 F216 A217 D218 E219 D222             E223 K224 V225 G226 L227 L232 S233 A234 S235 V236 A237 H243 E246 D247 N250 I251 L252

• Molecule 23: Ribosomal RNA small subunit methyltransferase NEP1 Page 33 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain E2:                MET VAL GLU ASP SER ARG VAL ARG ASP ALA LEU LYS GLY GLY ASP GLN LYS ALA LEU PRO ALA SER LEU VAL PRO GLN ALA P28 P29 V30 L31 T32 S33 K34 D35 K36 I37 T38 K39 R40 M41 I42 V43 V44 L45 A46 M47 A48 S49 L50 E51 T52 H53 K54 I55 SER SER ASN GLY PRO                         GLY GLY ASP K64 Y65 V66 L67 L68 N69 CYS D71 D72 H73 Q74 L77 K78 D83 E86 A87 R88 L96 L100 D101 S102 P103 I104 A107 G108 K109 L110 Q111 V112 Y113 I114 Q115 T116 S117 R118 G119 I120 L121 I122 E123 V124 I130 P131 R132 M141 V142 Q143 L144                            K147 I150 R151 E157 K158 L159 L160 K161 V162 I163 K164 I167 H170 L171 P172 T173 K174 C175 R176 K177 V178 T179 L180 D183 A184 P185 V189 Y192 I193 L196 D197 D198 D199 E200 C203 V204 F205 A208 M209 A210 R211 D214 N215 F216 E219 Y220 V221 D222            E223 K224 V225 G226 L227 S228 P231 L232 S233 A234 F241 A245 E246 W249 N250 I251 L252

• Molecule 24: Essential nuclear protein 1

Chain E3: MET ALA ARG ALA SER SER THR LYS ALA ARG LYS GLN ARG HIS ASP PRO LEU LEU LYS ASP LEU ASP ALA ALA GLN GLY THR LEU LYS LYS ILE ASN LYS LYS LYS LEU ALA GLN ASN ASP ALA ALA ASN HIS ASP ALA ALA ASN GLU GLU ASP GLY TYR ILE ASP SER LYS ALA SER ARG LYS ILE LEU GLN LEU ALA LYS GLU GLN GLN ASP GLU ILE GLU GLY GLU GLU LEU ALA GLU SER GLU ARG ASN LYS GLN PHE GLU ALA ARG PHE THR THR MET SER TYR ASP ASP GLU ASP GLU ASP GLU ASP GLU ASP GLU GLU ALA PHE GLY GLU ASP ILE SER ASP PHE GLU PRO GLU GLY ASP TYR LYS GLU GLU GLU GLU ILE VAL GLU ILE ASP GLU GLU ASP ALA ALA MET PHE GLU GLN TYR PHE LYS LYS SER ASP ASP PHE ASN SER LEU SER GLY SER TYR ASN LEU ALA ASP LYS ILE MET ALA SER ILE ARG GLU LYS GLU SER GLN VAL GLU ASP MET GLN ASP ASP                         GLU PRO LEU ALA ASN GLU GLN ASN THR SER ARG GLY ASN ILE SER SER GLY LEU LYS SER GLY GLU GLY VAL A205 L206 P207 E208 K209 V210 I211 K212 A213 Y214 T215 T216 L221 K222 T223 W224 T225 H226 G227 K228 L229 P230 K231 L232 F233 K234 V235 I236 P237 S238 L239 R240 N241 D244                         V248 T249 N250 P251 E252 E253 W254 S255 P256 H257 V258 V259 Y260 E261 A262 F266 V267 S268 N269 L270 Q276 I279 N280 L283 R286 F287 R288 D289 N290 I291 E292 T293 S294 E295 D296 H297 N300 Y301 H302 I303 Y304 R305 A306 V307 K308 K309 S310 L311 Y312 F318 K319 G320                                       F321 L322 F323 N331 V332 R333 E334 A335 A338 G339 S340 V341 L342 A343 K344 V345 S346 A349 L350 H351 S352 S353 L356 R361 L362 P363 F364 S365 F371 L376 D377 K378 K379 Y380 A381 D388 D389 C390 F397 R398 I399 L400 D401 ASP G403 S404 N405 G406 E407 T410                         R411 V412 L413 W417 H418 L422 Y428 D431 I432 T433 Q434 D435 Q436 F439 L440 L441 E442 T443 V444 R447 G448 H449 K450 D451 I452 G453 P454 E455 I456 R457 R458 E459 L460 L461 A462 S465 ARG GLU PHE VAL ASP PRO GLN GLU ALA ASN ASP ASP LEU MET ILE ASP VAL ASN

• Molecule 25: Enp2

Chain E4: Page 34 Full wwPDB EM Validation Report EMD-6695, 5WYJ                                        X1 X2 X3 X10 X11 X12 X13 X14 X18 X19 X20 X21 X22 X23 X31 X32 X33 X34 X41 X42 X48 X49 X50 X54 X55 X56 X57 X58 X59 X68 X69 X70 X71 X72 X73 X74 X75 X76 X77 X93 X94 X95 X96 X100 X103 X106 X107 X108 X109                              X110 X111 X112 X113 X114 X115 X116 X120 X126 X133 X134 X138 X139 X140 X141 X145 X146 X147 X150 X151 X155 X156 X157 X158 X159 X160 X165 X166 X167 X168 X171 X172 X173 X174 X175 X176 X177 X178 X179 X180 X184 X185 X186 X187 X190 X193 X194                         X197 X198 X199 X200 X201 X211 X212 X213 X214 X215 X216 X217 X218 X219 X220 X221 X222 X226 X227 X228 X229 X230 X233 X234 X238 X239 X240 X244 X258 X259 X265 X268 X272 X276 X277 X283 X284 X285 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 26: KRR1 small subunit processome component

Chain K1:              MET VAL SER THR HIS ASN ARG ASP LYS PRO TRP ASP THR ASP ASP ILE ASP LYS TRP LYS ILE GLU GLU PHE LYS GLU GLU ASP ASN ALA SER GLY GLN PRO PHE ALA GLU E38 S39 M42 T43 L44 F45 P46 K47 Y48 R49 T61 R62 A63 L64 D65 A70 C71 D74 L75                          V76 E77 T84 T85 R86 K87 P91 A92 A97 R98 D99 L100 L103 R106 S107 V108 P109 F110 L117 Q118 D119 D120 C123 D124 V125 I126 K127 I128 G129 N130 F131 V132 T133 N134 K135 E136 R137 F138 V139 K140 R141 R142 Q143 R144 L145 V146 N149 G150 N151 T152 L153 K154                         A155 L158 L159 T160 K161 C162 Y163 I164 L165 V166 Q167 V171 S172 A173 M174 G175 L180 V183 R184 V187 E188 D189 C190 M191 K192 N193 I194 H195 P196 I197 Y198 H199 E202 L203 R207 E208 R212 PRO GLU LEU ALA ASN GLU ASP TRP SER ARG PHE LEU PRO MET PHE LYS LYS ARG ASN VAL ALA ARG LYS LYS PRO LYS LYS ILE ARG ASN VAL GLU LYS LYS VAL TYR THR PRO PHE PRO PRO ALA GLN LEU PRO ARG LYS VAL ASP LEU GLU ILE GLU SER GLY GLU TYR PHE LEU SER LYS ARG GLU LYS GLN MET LYS LYS LEU ASN GLU GLN LYS GLU LYS GLN MET GLU ARG GLU ILE GLU ARG GLN GLU GLU ARG ALA LYS ASP PHE ILE ALA PRO GLU GLU GLU ALA TYR LYS PRO ASN GLN ASN

• Molecule 27: U3 small nucleolar ribonucleoprotein protein IMP3 Page 35 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain MA:            MET VAL ARG LYS LEU LYS HIS HIS GLU GLN LYS LEU LEU LYS LYS VAL ASP PHE LEU GLU TRP LYS GLN ASP GLN GLY HIS ARG D29 Y44 Y47 N48 R49 I50 R55 T67 F70 R71 R72 K73 H74 D80 T89 T90 K91 S92 K93 V101 R108 L111              P112 V113 I114 A121 E122 E132 Q133 G134 H135 V138 G139 P140 I143 N144 D145 P146 A147 Y148 L149 V150 E155 V158 V161 ASP ASN SER LYS ILE LYS LYS THR LEU LEU ARG TYR ARG ASN GLN ILE ASP ASP PHE ASP PHE SER

• Molecule 28: U3 small nucleolar ribonucleoprotein protein IMP4

Chain MB: MET LEU ARG ARG GLN ALA ARG GLU ARG ARG GLU TYR LEU TYR ARG LYS ALA GLN GLU LEU GLN ASP SER GLN LEU GLN GLN LYS ARG GLN ILE ILE LYS GLN ALA LEU ALA GLN GLY LYS PRO LEU PRO LYS GLU LEU ALA GLU ASP GLU SER LEU GLN LYS ASP PHE ARG TYR ASP GLN                 SER LEU LYS GLU SER GLU GLU ALA ASP ASP LEU GLN VAL ASP ASP GLU TYR ALA ALA THR SER GLY ILE M84 D85 P86 R94 L100 F103 L109 L110 F111 P112 N113 R116 L117 N118 Y122 V123 M124 P125 N126 G135 H143 E144 H145 R146 P149 T150 S151 L152                         T162 F165 S166 L167 H168 N169 V170 V171 M172 R173 I176 I177 N181 Q182 S183 E184 V185 N186 P187 I190 F191 D192 N193 F194 A197 K200 I205 L209 F210 P214 K215 R220 A225 N226 R227 G228 D229 V233 R234 Q235 H236 T241 V245 E246 I247         A248 E249 V250 G251 P252 R253 F254 E255 M256 R257 L258 L261 R262 L263 G264 T265 L266 E267 ASN LYS ASP ALA ASP VAL GLU TRP GLN LEU ARG ARG PHE ILE ARG THR ALA ASN LYS LYS ASP TYR LEU

• Molecule 29: Mpp10,U3 small nucleolar RNA-associated protein MPP10

Chain MC:   UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X45 X54 X58 X63 X64 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK E429 K432 Page 36 Full wwPDB EM Validation Report EMD-6695, 5WYJ V454 HIS PHE VAL PRO LYS PRO ALA SER THR SER LEU GLU ILE ARG VAL GLU THR PRO THR ILE SER MET GLU ASP ALA GLN PRO LEU TYR MET SER ASN ALA SER SER LEU ALA PRO GLN GLU ILE TYR ASN VAL GLY LYS ALA GLU LYS ASP GLY GLU ILE ARG LEU LYS ASN GLY VAL ALA MET SER LYS GLU GLU LEU THR ARG GLU ASP LYS ASN ARG LEU ARG ARG ALA LEU LYS ARG LYS ARG SER LYS ALA ASN LEU PRO ASN VAL ASN LYS ARG SER LYS ARG ASN ASP VAL VAL ASP THR LEU SER LYS ALA LYS ASN ILE THR VAL ILE ASN GLN LYS GLY GLU LYS LYS ASP VAL SER GLY LYS THR LYS LYS SER ARG SER GLY PRO ASP SER THR ASN ILE LYS LEU

• Molecule 30: Pre-rRNA-processing protein PNO1

Chain P1: MET VAL ALA PRO THR ALA LEU LYS LYS ALA THR VAL THR PRO VAL SER GLY GLN ASP GLY GLY SER SER ARG ILE ILE GLY ILE ASN ASN THR GLU SER ILE ASP GLU ASP ASP ASP ASP ASP VAL LEU LEU ASP ASP SER ASP ASN ASN THR ALA LYS GLU GLU VAL GLU GLY GLU GLU              GLY SER ARG LYS THR HIS GLU SER LYS THR VAL VAL VAL ASP ASP GLN GLY LYS PRO ARG PHE THR SER ALA SER LYS THR GLN GLY ASN LYS ILE LYS F94 P103 H104 R105 M106 L109 W113 H123 L126 T134 K135 S136 N142 P143 K144 F145 T146 T147 D148 P149                            L152 F158 A161 F162 T163 L164 G165 F166 D170 L174 L175 R176 L177 D178 D179 F185 E186 V187 K188 K191 T192 L193 T194 G195 D196 H197 L198 A201 I202 G203 R204 I205 A219 T220 R221 T222 H232 F237 I240 V248 I251 S254 P255 P256 V266 ALA SER ARG LEU LYS GLU ARG TYR

• Molecule 31: RNA 3’-terminal phosphate cyclase-like protein

Chain R1:               MET SER SER SER ALA PRO K7 Y8 T9 T10 F11 S14 F17 R18 L19 R20 L23 L26 K29 P30 I31 K32 I33 G39 D40 L41 F52 E66 I67 V74 I75 Y76 R77 I81 A85 H88 I89 C90 V96 G97 Y98 E101 P102 Y105 L106                             A107 P108 F109 S110 K111 F114 K119 G120 I121 T122 D127 A128 W135 G136 L137 V140 K143 E148 C149 A150 T153 L154 K155 R156 P159 P160 E165 V166 V169 V170 D171 S172 L173 I174 A175 Q176 P177 I178 T179 R185 P186 I187 I188 T192 T198 R199                          V200 S201 P202 S203 L204 V205 N206 R207 M208 L216 L219 E222 A227 D228 V229 W230 R231 N234 S235 G236 Q248 K252 Y256 F257 A258 E259 D260 I261 G262 D263 A264 G265 L271 Q278 E281 K285 S286 A287 A288 V289 G290 R291 M301 G304 K305 E306                    D307 I308 G309 R310 L311 R312 I313 E316 E320 R321 I332 F333 N334 T335 D343 E344 A345 D346 N347 E348 D349 M350 I351 A352 T353 I354 K355 G358 F359 T360 N361 THR SER LYS LYS ILE ALA

• Molecule 32: rRNA biogenesis protein RRP5

Chain R2: MET VAL ALA SER THR LYS ARG LYS ARG ASP GLU ASP PHE PRO LEU SER ARG GLU ASP SER THR LYS GLN PRO SER THR SER SER LEU VAL ARG ASN THR GLU GLU VAL SER PHE PRO ARG GLY GLY ALA SER ALA LEU THR PRO LEU GLU LEU LYS GLN VAL ALA ASN GLU ALA ALA SER ASP VAL LEU PHE GLY ASN GLU SER VAL LYS ALA SER GLU PRO ALA SER ARG PRO LEU LYS LYS LYS LYS THR THR LYS LYS SER THR SER LYS ASP SER GLU ALA SER SER ALA ASN SER ASP GLU ALA ARG ALA GLY LEU ILE GLU HIS VAL ASN PHE LYS THR LEU LYS ASN GLY SER Page 37 Full wwPDB EM Validation Report EMD-6695, 5WYJ SER LEU LEU GLY GLN ILE SER ALA ILE THR LYS GLN ASP LEU CYS ILE THR PHE THR ASP GLY ILE SER GLY TYR VAL ASN LEU THR HIS ILE SER GLU GLU PHE THR SER ILE LEU GLU ASP LEU ASP GLU ASP MET ASP SER ASP THR ASP ALA ALA ASP GLU LYS LYS SER LYS VAL GLU ASP ALA GLU TYR GLU SER SER ASP ASP GLU ASP GLU LYS LEU ASP LYS SER ASN GLU LEU PRO ASN LEU ARG ARG TYR PHE HIS ILE GLY GLN TRP LEU ARG CYS SER VAL ILE LYS ASN THR SER LEU GLU PRO SER THR LYS LYS SER LYS LYS LYS ARG ILE GLU LEU THR ILE GLU PRO SER SER VAL ASN ILE TYR ALA ASP GLU ASP LEU VAL LYS SER THR SER ILE GLN CYS ALA VAL LYS SER ILE GLU ASP HIS GLY ALA THR LEU ASP VAL GLY LEU PRO GLY PHE THR GLY PHE ILE ALA LYS LYS ASP PHE GLY ASN PHE GLU LYS LEU LEU PRO GLY ALA VAL PHE LEU GLY ASN ILE THR LYS LYS SER ASP ARG SER ILE VAL VAL ASN THR ASP PHE SER ASP LYS LYS ASN LYS ILE THR GLN ILE SER SER ILE ASP ALA ILE ILE PRO GLY GLN ILE VAL ASP LEU LEU CYS GLU SER ILE THR LYS ASN GLY ILE ALA GLY LYS VAL PHE GLY LEU VAL SER GLY VAL VAL ASN VAL SER HIS LEU ARG THR PHE SER GLU GLU ASP LEU LYS HIS LYS PHE VAL ILE GLY SER SER ILE ARG CYS ARG ILE ILE ALA CYS LEU GLU ASN LYS SER GLY ASP LYS VAL LEU ILE LEU SER ASN LEU PRO HIS ILE LEU LYS LEU GLU ASP ALA LEU ARG SER THR GLU GLY LEU ASP ALA PHE PRO ILE GLY TYR THR PHE GLU SER CYS SER ILE LYS GLY ARG ASP SER GLU TYR LEU TYR LEU ALA LEU ASP ASP ASP ARG LEU GLY LYS VAL HIS SER SER ARG VAL GLY GLU ILE GLU ASN SER GLU ASN LEU SER SER ARG VAL LEU GLY TYR SER PRO VAL ASP ASP ILE TYR GLN LEU SER THR ASP PRO LYS TYR LEU LYS LEU LYS TYR LEU ARG THR ASN ASP ILE PRO ILE GLY GLU LEU LEU PRO SER CYS GLU ILE THR SER VAL SER SER SER GLY ILE GLU LEU LYS ILE PHE ASN GLY GLN PHE LYS ALA SER VAL PRO PRO LEU HIS ILE SER ASP THR ARG LEU VAL TYR PRO GLU ARG LYS PHE LYS ILE GLY SER LYS VAL LYS GLY ARG VAL ILE SER VAL ASN SER ARG GLY ASN VAL HIS VAL THR LEU LYS LYS SER LEU VAL ASN ILE GLU ASP ASN GLU LEU PRO LEU VAL SER THR TYR GLU ASN ALA LYS ASN ILE LYS GLU LYS ASN GLU LYS THR LEU ALA THR ILE GLN VAL PHE LYS PRO ASN GLY CYS ILE ILE SER PHE PHE GLY GLY LEU SER GLY PHE LEU PRO ASN SER GLU ILE SER GLU VAL PHE VAL LYS ARG PRO GLU GLU HIS LEU ARG LEU GLY GLN THR VAL ILE VAL LYS LEU LEU ASP VAL ASP ALA ASP ARG ARG ARG ILE ILE ALA THR CYS LYS VAL SER ASN GLU GLN ALA ALA GLN GLN LYS ASP THR ILE GLU ASN ILE VAL PRO GLY ARG THR ILE ILE THR VAL HIS VAL ILE GLU LYS THR LYS ASP SER VAL ILE VAL GLU ILE PRO ASP VAL GLY LEU ARG GLY VAL ILE TYR VAL GLY HIS LEU SER ASP SER ARG ILE GLU GLN ASN ARG ALA GLN LEU LYS LYS LEU ARG ILE GLY THR GLU LEU THR GLY LEU VAL ILE ASP LYS ASP THR ARG THR ARG VAL PHE ASN MET SER LEU LYS SER SER LEU ILE LYS ASP ALA LYS LYS GLU THR LEU PRO LEU THR TYR ASP ASP VAL LYS ASP LEU ASN LYS ASP VAL PRO MET HIS ALA TYR ILE LYS SER ILE SER ASP LYS GLY LEU PHE VAL ALA PHE ASN GLY LYS PHE ILE GLY LEU VAL LEU PRO SER TYR ALA VAL ASP SER ARG ASP ILE ASP ILE SER LYS ALA PHE TYR ILE ASN GLN SER VAL THR VAL TYR LEU LEU ARG THR ASP ASP LYS ASN GLN LYS PHE LEU LEU SER LEU LYS ALA PRO LYS VAL LYS GLU GLU LYS LYS LYS VAL GLU SER ASN ILE GLU ASP PRO VAL ASP SER SER ILE LYS SER TRP ASP ASP LEU SER ILE GLY SER ILE VAL LYS ALA LYS ILE LYS SER VAL LYS LYS ASN GLN LEU ASN VAL ILE LEU ALA ALA ASN LEU HIS GLY ARG VAL ASP ILE ALA GLU VAL PHE ASP THR TYR GLU GLU ILE THR ASP LYS LYS GLN PRO LEU SER ASN TYR LYS LYS ASP ASP VAL ILE LYS VAL LYS ILE ILE GLY ASN HIS ASP VAL LYS SER HIS LYS PHE LEU PRO ILE THR HIS LYS ILE SER LYS ALA SER VAL LEU GLU LEU SER MET LYS PRO SER GLU LEU LYS SER LYS GLU VAL HIS THR LYS SER LEU GLU GLU ILE ASN ILE GLY GLN GLU LEU THR GLY PHE VAL ASN ASN SER SER GLY ASN HIS LEU TRP LEU THR ILE SER PRO VAL LEU LYS ALA ARG ILE SER LEU LEU ASP LEU ALA ASP ASN ASP SER ASN PHE SER GLU ASN ILE GLU SER VAL PHE PRO LEU GLY SER ALA LEU GLN VAL LYS VAL ALA SER ILE ASP ARG GLU HIS GLY PHE VAL ASN ALA ILE GLY LYS SER HIS VAL ASP ILE ASN MET SER THR ILE LYS VAL GLY ASP GLU LEU PRO GLY ARG VAL LEU LYS ILE ALA GLU LYS TYR VAL LEU LEU ASP LEU GLY ASN LYS VAL THR GLY ILE SER PHE ILE THR ASP ALA LEU ASN ASP PHE SER LEU THR LEU LYS GLU ALA PHE GLU ASP LYS ILE ASN ASN VAL ILE PRO THR THR VAL LEU SER VAL ASP GLU GLN ASN LYS LYS ILE GLU LEU SER LEU ARG PRO ALA THR ALA LYS THR ARG SER ILE LYS SER HIS GLU ASP LEU LYS GLN GLY GLU ILE VAL ASP GLY ILE VAL LYS ASN VAL ASN ASP LYS GLY ILE PHE VAL TYR LEU SER ARG LYS VAL GLU ALA PHE VAL PRO VAL SER LYS LEU SER ASP SER TYR LEU LYS GLU TRP LYS LYS PHE TYR LYS PRO MET GLN TYR VAL LEU GLY LYS VAL VAL THR CYS ASP GLU ASP SER ARG ILE SER LEU THR LEU ARG GLU SER GLU ILE ASN GLY ASP LEU LYS VAL LEU LYS THR TYR SER ASP ILE LYS ALA GLY ASP VAL PHE GLU GLY THR ILE LYS SER VAL THR ASP PHE GLY VAL PHE VAL LYS LEU ASP ASN THR VAL ASN VAL THR GLY LEU ALA HIS ILE THR GLU ILE ALA ASP LYS LYS PRO GLU ASP LEU SER ALA LEU PHE GLY VAL GLY ASP ARG VAL LYS ALA ILE Page 38 Full wwPDB EM Validation Report EMD-6695, 5WYJ VAL LEU LYS THR ASN PRO GLU LYS LYS GLN ILE SER LEU SER LEU LYS ALA SER HIS PHE SER LYS GLU ALA GLU LEU ALA SER THR THR THR THR THR THR THR VAL ASP GLN LEU GLU LYS GLU ASP GLU ASP GLU VAL MET ALA ASP ALA GLY PHE ASN ASP SER ASP SER GLU SER            ASP ILE GLY ASP GLN ASN THR GLU VAL ALA ASP ARG LYS PRO GLU THR SER SER ASP GLY LEU SER LEU SER ALA GLY PHE D1408 W1409 T1410 A1411 S1412 I1413 L1414 D1415 Q1416 A1417 Q1418 GLU GLU GLU GLU SER ASP GLN ASP GLN GLU ASP PHE THR GLU ASN LYS LYS HIS LYS HIS LYS ARG                                         ARG LYS GLU ASN VAL VAL GLN ASP LYS THR ILE ASP ILE ASN THR ARG A1457 P1458 E1459 S1460 V1461 A1462 D1463 F1464 E1465 R1466 L1467 L1468 I1469 G1470 N1471 P1472 N1473 S1474 S1475 V1476 V1477 W1478 M1479 N1480 Y1481 M1482 A1483 F1484 Q1485 L1486 Q1487 L1488 S1489 E1490 I1491 E1492 K1493 A1494 R1495 E1496 L1497 A1498 E1499 R1500                                                      A1501 L1502 K1503 T1504 I1505 N1506 F1507 R1508 E1509 E1510 A1511 E1512 K1513 I1516 W1517 I1518 L1521 N1522 L1523 E1524 N1525 T1526 F1527 G1528 T1529 T1532 L1533 E1534 E1535 V1536 F1537 S1538 R1539 A1540 C1541 Q1542 Y1543 M1544 D1545 S1546 Y1547 T1548 I1549 H1550 T1551 K1552 L1553 L1554 G1555 I1556 Y1557 E1558 I1559 S1560 E1561 K1562 F1563                                                             D1564 K1565 A1566 A1567 E1568 L1569 F1570 K1571 A1572 T1573 A1574 K1575 K1576 F1577 G1578 G1579 E1580 K1581 V1582 S1583 I1584 W1585 V1586 S1587 W1588 G1589 D1590 F1591 L1592 I1593 S1594 H1595 N1596 E1597 E1598 Q1599 E1600 A1601 R1602 T1603 I1604 L1605 G1606 N1607 A1608 L1609 K1610 A1611 L1612 P1613 K1614 R1615 N1616 H1617 I1618 E1619 V1620 V1621 R1622 K1623                                                             F1624 A1625 Q1626 L1627 E1628 F1629 A1630 K1631 G1632 D1633 P1634 E1635 R1636 G1637 R1638 S1639 L1640 F1641 E1642 G1643 L1644 V1645 A1646 D1647 A1648 P1649 K1650 R1651 I1652 D1653 L1654 W1655 N1656 V1657 Y1658 V1659 D1660 Q1661 E1662 V1663 K1664 A1665 K1666 D1667 K1668 K1669 K1670 V1671 E1672 D1673 L1674 F1675 E1676 R1677 I1678 I1679 T1680 K1681 K1682 I1683                                   T1684 R1685 K1686 Q1687 A1688 K1689 F1690 F1691 F1692 N1693 K1694 W1695 L1696 Q1697 F1698 E1699 E1700 S1701 GLU GLY ASP GLU K1706 T1707 I1708 E1709 Y1710 V1711 K1712 A1713 K1714 A1715 T1716 E1717 Y1718 V1719 A1720 S1721 HIS GLU SER GLN LYS ALA ASP GLU

• Molecule 33: Sof1

Chain S1: UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK         UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X101 X111 X112 X113 X114 X115 X116 X117 X118 X121 X126 X127 X128 X137 X138                  X145 X146 X157 X158 X159 X162 X163 X167 X168 X169 X170 X171 X175 X178 X179 X180 X184 X185 X186 X187 X195 X196 X199 X200 X203 X204 X208 X209 X210 X211 X212 X218 X219 X220 X221 X225 X226 X227 X235 X236 X237 X238 X239 X240 X241 X242 X243                           X244 X245 X246 X247 X248 X249 X250 X251 X252 X253 X254 X255 X261 X272 X273 X276 X277 X278 X279 X282 X289 X290 X291 X292 X295 X296 X297 X301 X302 X303 X307 X308 X309 X310 X316 X317 X318 X319 X320 X334 X342 X350 X351 X357 X358 X359 X360 X364 X371 X372 X373 X384 X385 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 34: 18S ribosomal RNA Page 39 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain SA:    U A U C U G G7 U8 U9 G10 A11 U15 G16 C17 C A G U A22 C25 A26 U27 G34 U35 C38 A39 A A G A U U A46 A47 C50 A51 C54 A U G U C U A A G U A U A A G C A              A U U U A U A C A G U G A A A C U G C G A92 A93 U94 G95 G96 A100 U101 U102 A103 A104 A105 U106 C107 A108 U111 C114 G115 U116 U117 A126 G127 U128 U129 U132 U133 U134 A135 C136 U137 A138 C139 A140 A145 U146         G151 U152 G153 G154 U155 A156 A157 U158 U159 C160 U161 A162 G163 U170 A173 U174 G175 C176 U177 U178 A179 A180 A181 C184 U185 C186 G187 A188 C189 C190 C191 U192 U193 U194 G195 G196 G199 A200 G201 A202 U203 G204 U205 A206 A215 U216 A A A A A A U C            A A U G U C U U C G G A C U C U U U G A U G A U U249 C250 A251 U252 A253 A254 U255 A256 A257 U260 U261 G264 A265 A266 C270 A271 U272 G273 G274 C275 C276 U277 U278 G279 U280 G281 C282 U283 G284 G285 A288 U289      G290 G291 C294 A295 U296 U297 C298 A299 U302 C308 U313 A316 C321 G322 A323 U327 A328 G329 G330 A331 U332 U335 G336 G337 C338 C339 U340 A341 U345 U348 U349 U350 C351 A352 A353 C354 G355 G356 G357 U358 A359 A360 C361 G362 G363 G364 G365 U368 A369                A370 G371 G372 G373 U374 U375 C376 G377 A378 U379 U380 C381 C382 G383 G384 A387 G388 G389 G390 A391 G392 C393 C394 U395 G396 A399 A400 A401 C402 G403 G404 C405 U406 A407 C411 A416 A417 G418 G423 C A G426 C427 A428 G429 G430 C431 G432 C433 G C A A A U439          U440 A441 C442 C443 C444 A445 A446 A452 U453 U454 C455 G459 A460 G461 G462 U466 G467 A468 C469 A470 A475 U476 A477 A478 U482 A483 C484 A485 G486 G487 G488 C491 A492 U493 U494 G497 G498 U501 U502 G503 U504 A505 A506 U507 U508 G509 G510 A511 A512 A515  A518 U522 G523 U524 A525 A526 A527 U528 A529 C530 C531 U532 G537 A538 G539 G540 A541 A542 C543 A544 A545 U546 U547 G548 G549 A550 G G G C A A G557 U558 C559 U560 G561 G562 U563 G C C566 A567 G568 C569 A570 G571 C572 C573 G574 C575 G576 G U A A U U  C C584 A585 A591 A592 U593 A594 G595 A605 A G U U G U U G C A G U U A A A A A G624 U629 A630 A635 U638 U U G G G C C C G G U U G G C C G G U C C G A U U U U U U C G U G U A C U G G A U U U C C A A C G G G G C C U U U C C U U C U G G C U A A C C U U G A G U C C U U G U G G C U C U U G G C G A A C C A G G A C U U U U A C U U U G A A A A A A U U A G A G U G U U C A A A G C A G G C G U A U U G C U C G A A U A U A U U A G C A U G G A A U A A U A G A A U A G G A C G U U U G G U U C U A U U U U G U U G G U U U   C U A G G A C C A U C G U A A U G A U860 U861 A862 A863 U864 A865 G866 G867 G868 A869 C870 G871 G872 U873 G876 G877 G878 C883 A884 G885 U886 A887 C890 A891 A892 U893 U894 G895 U896 C897 A898 G899 A900 G901 U908 U912 G913    G914 U921 G922 A923 A924 G925 U928 A929 A930 C931 U932 A933 C934 U935 G936 C937 G938 A941 G942 U947 G948 A951 A955 U959 U960 U961 U964 U965 A966 A967 U968 C969 C975 G976 A977 A978 A G U U A G G G G A U C G A A Page 40 Full wwPDB EM Validation Report EMD-6695, 5WYJ  G A U G A U C A G A U A C C G U C G U A G U C U U A A C C A U A A A C U A U G C C G A C U1038 A1039 G1040 G1041 G1042 A1043 U1044 C1045 G1046 U1049 G1050 G1051 U1052 G1053      U1058 U1059 U1060 A1061 A1062 U1063 C1066 C1072 G1073 G1074 C1075 A1076 U1080 A1081 C1082 G1083 A1084 G1085 A1086 A1087 A1088 U1089 C1090 A A A G U C U U U G G G U U C U G G G G G1111 G1112 U1117 G1118 G1119 A1124 A1125 G G C U G    A A A C U U A A A G G A A U U1145 G1150 A1151 A1152 G1153 G1154 G1155 C1156 A1157 C1158 C1159 A1160 A1163 G1164 G1165 A1166 G1167 C1173 C1174 U1175 G1176 C1177 G1178 G1179 C1180 U U A A U U U G A1189 C1190 U1191 C1192 A1193 C1197 G G G                             G1201 A1202 A1203 A1204 C1205 U1206 C1207 A1208 C1209 C1210 A1211 G1212 G1213 C1216 A1217 G1218 A1219 C1220 A1224 U1225 A1226 A1227 G1228 G1229 G1233 A1234 C1235 A1236 G1237 A1238 U1239 U1240 G1241 A1242 G1243 A1244 G1245 C1246 U1247 C1248 U1249 U1250 U1251 C1252 U1253 A1256 U1257 U1258 G1263 G1264 G1265 U1266 G1267 G1268 U1269     G1270 G1271 U1272 G1273 C1274 A1275 U1276 G1277 G1278 G1281 U1282 U C U U A G U U G G U G G A G U G A U U U G U C U G C U U A A U U G C G A U A A C G A A C G A G A C C U U A A C C U A C U A A A U A G U G G U G C U A G C A U U U G C U G G U U A U C C A C U U C U U A G A G G G A C U                  A U C G G U U U C A A G C C G A U G G A A G U U U G A G G C A A U A A1425 C1426 A1427 G1428 G1429 U1430 C1431 U1432 G1433 U1434 G1435 A1436 U1437 C1440 C1441 U1442 U1443 A1444 G1445 A1446 C1447 G1448 U1449 U1450                 G1453 C1456 C1457 G C A1460 C1461 G1462 C1463 G1464 C1465 A1471 C1472 U1473 G1474 A1475 C1481 C1482 A1483 G1484 C1485 G1486 A1487 G1488 U C U A A C1494 C1495 U1496 U1497 G1498 G1499 C1500 C1501 G1502 A1503 G1504 A1505 G1506 U1510 U1511 G1512 G1513 U1514 A1515 A1516 U C U U G U G  A1524 A1525 A1526 C1527 U1528 C1529 U1535 G1536 C1537 U G G G G A U A G A G C A U U G U A A U U A U U G C U C U U C A1569 A1570 C1571 G1572 A1573 G1574 G1575 C1581 U1582 A1583 G1584 U1585 A1586 A1587 G1588 A1593 G1594         U1595 C1596 C1599 A1600 G1601 U1604 G1605 C1606 G1607 U1608 U1609 G1610 A1611 U1612 U1613 A1614 C1615 G1616 U1617 C1618 C1619 C1620 C1623 C1624 C1625 U1628 G1629 U1630 A1631 C1632 A1633 C1636 C1637 G1638 U1647 A1648 G1649 U1650 A1651 C1652 C1653 U1657 G1658 U1661 G1662 G1663 C1664 U1665 U1666 C1674              A1678 G1679 C1683 U1684 G1685 C1686 U U A G A G A A G G G G G C A A C U C C A U C U C A G A G1715 C1716 G1717 G1718 A1719 G1720 A1721 A1722 U1723 U1724 U1725 G1726 G1727 A1728 C1729 A1730 C1733 U1734 U1735 U1738 C1739 A1740    G1748 A1749 A1754 A1755 A1756 G1757 U1758 C1759 G1760 A1763 C1764 A1765 A1766 G1767 G U U U C C G U A G G U G A A C C U G C G G A A G G A U1795 C1796 A1802 G1803 A1804 A1805 A1806 U1807 U1808 U1812

• Molecule 35: 40S ribosomal protein S1-A

Chain SC: Page 41 Full wwPDB EM Validation Report EMD-6695, 5WYJ                        MET ALA VAL GLY LYS ASN LYS ARG LEU SER LYS GLY LYS LYS GLY GLN LYS LYS ARG V20 V21 D22 P23 F24 T25 D31 I32 K33 A34 P35 S36 T37 F38 E39 N40 R41 N42 V43 G44 K45 T46 L47 V48 N49 K50 S51 L54 K55 S56 A57 S58 D59 A60 L61 V65 V66 E67                              V68 C69 L70 A71 D72 L73 Q74 G75 S76 E77 D78 H79 R82 K83 R87 V88 D89 E90 V91 Q92 L96 L97 T98 N99 M103 D104 F105 T106 T107 D108 K109 L110 R111 S112 M113 V114 R115 K116 W117 Q118 T119 L120 A123 N124 V127 K128 T129 S130 D131 D132 Y133 V134 A139                  I140 A141 F142 T143 R144 K145 Q146 A147 N148 Q149 V150 K151 R152 H153 S154 S158 I161 R162 R165 L172 V176 Q177 G178 S179 T180 L181 A182 P190 E191 V192 I193 N194 K195 E196 N199 A200 T201 K202 D203 I204 F205 P206 L207 Q208 N209 I210 H211 V212 R213 K214 V215 K216 L217       L218 K219 Q220 P221 K222 F223 D224 V225 H232 G233 GLU GLY SER GLY GLU GLU LYS GLY LYS LYS VAL THR GLY PHE LYS ASP GLU VAL LEU GLU THR VAL

• Molecule 36: 40S ribosomal protein S4-A

Chain SF:                         MET ALA ARG GLY PRO LYS LYS HIS L9 K10 R11 L12 A13 A14 P15 H16 H17 W18 L19 L20 D21 K22 L23 S24 G25 C26 P29 R30 P31 S32 A33 G34 P35 H36 K37 L38 R39 E40 S41 F47 L48 R49 N50 K53 Y54 A55 R59 E60 V61 K62 L65 M66 V70 K71                      V72 D73 G74 K75 V76 R77 T78 D79 T80 T81 Y82 P83 A84 G85 D88 V89 I90 T91 L92 D93 A94 E97 R100 L101 D104 V105 K106 G107 R108 F109 A110 V111 H112 D116 E117 E118 A119 S120 Y121 K122 L123 V126 K127 K128 V129 Q130 K133 V136 P137 Y138 V139 V140                             T141 H142 D143 G144 R145 T146 I147 R148 Y149 P150 D151 P152 N153 I154 K155 V160 L164 A165 S166 G167 K168 K174 F175 D176 A177 G178 K179 L180 V181 Y182 V183 T184 G185 G190 R191 I192 G193 T194 I195 K198 E199 D202 G203 G204 F205 D206 I210 K211 D212 S213 L214 D215 N216             T217 F218 L222 V225 F226 V227 I228 Q231 I236 S237 K240 G243 I244 K245 LEU SER ILE ALA GLU GLU ARG ASP ARG ARG ARG ALA GLN GLN GLY LEU

• Molecule 37: 40S ribosomal protein S5

Chain SG:                 MET SER ASP THR GLU ALA PRO VAL GLU VAL GLN GLU ASP PHE GLU VAL VAL GLU GLU F20 T21 A26 Q37 T38 K41 F48 E49 E50 V51 E52 V53 K54 D55 R65 Q66 P67 I68 F69 V70 A71 H72 T73 A74 G75 R76 Y77 A78 N79 K80 R81 F82 R83 K84 A85 Q86                     R92 L93 T94 N95 S96 L97 R102 N103 N104 G105 K106 K107 L108 K109 R112 I113 I114 N122 T125 D126 Q127 N128 P129 I130 Q131 T140 G141 P142 D145 T146 T147 R148 V149 G150 G151 G152 G153 A154 A155 R156 R157 Q158 A159 R166 R167 R180 E181 A182 A183 I187      A191 N200 A201 G204 S208 L217 E218 A221 R225

• Molecule 38: 40S ribosomal protein S6-A Page 42 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain SH:                                    M1 K2 L3 N4 I5 S6 G11 S12 Q13 K14 T15 I18 D19 D20 I24 R25 V26 F27 F28 D29 K30 R31 I32 G33 Q34 E35 V36 D37 A40 V41 G42 D43 E44 F45 Y48 K51 I52 S53 G54 G55 N56 D57 K58 Q59 G60 F61 P62 M63 K64 Q65 G66 V67 L68 L69                                    P70 T71 R72 I73 L76 L77 T78 K79 N80 V81 S82 C83 Y84 R85 P86 R87 R88 D89 G90 E91 R92 R98 G99 A100 I101 V102 G103 P104 D105 L106 A107 V108 L109 A110 V114 K115 E118 Q119 E120 L121 E122 G123 D126 T127 T128 VAL PRO LYS ARG LEU GLY PRO LYS ARG ALA        ASN ASN ILE ARG LYS PHE PHE GLY LEU SER LYS GLU ASP ASP VAL ARG ASP PHE VAL ILE ARG ARG GLU VAL THR LYS GLY GLU LYS THR TYR THR LYS ALA PRO LYS ILE GLN ARG LEU VAL THR P181 Q182 R183 L184 Q185 R186 K187 R188 H189 Q190 R191 K194 V195 R196 Q199                  R202 E207 Y208 A209 Q210 L211 L212 A213 K214 R215 L216 S217 E218 R219 K220 A221 E222 K223 A224 E225 I226 ARG LYS ARG ARG ALA SER SER LEU LYS ALA

• Molecule 39: 40S ribosomal protein S7-A

Chain SI:                                  MET SER ALA P4 Q5 A6 K7 I8 L9 S10 Q11 A12 P13 T14 E15 L16 E17 A21 F24 V25 E26 L27 E28 N29 S30 S31 P32 E33 L34 E37 L38 L41 Q42 F43 K44 S45 I46 R47 E48 I49 D50 V51 A52 G53 K56 A57 L58 V62 P63 V64 L67 H71                   K76 L77 T78 R79 E80 L81 E82 K83 K84 D87 R88 H89 A94 E95 R96 R97 I98 LEU PRO LYS PRO SER ARG THR SER ARG GLN VAL GLN LYS ARG PRO ARG SER ARG THR L118 H122 I125 L129 V130 F131 P132 T133 E134 I135 V136 G137 K138 R139 V140 R141 Y142 L143               V144 G145 G146 N147 K148 I149 Q150 L153 L154 D155 S156 K157 D158 V159 Q160 Q161 I162 D163 Y173 G178 K179 Q180 I181 V182 F183 E184 I185 P186 S187 GLU THR HIS

• Molecule 40: 40S ribosomal protein S8-A

Chain SJ:                             MET G2 I3 S4 R5 D6 S7 R8 H9 K10 R11 S12 A13 T14 K17 R18 A19 R22 K23 K24 R25 K26 F27 E28 L29 G30 P33 A34 N35 T36 K37 I38 G39 A40 K41 R42 I43 H44 T48 N52 Y55 R56 A57 L58 R59 I60 E61 T62 G63 N64 F65 S66 W67 A68                    S69 E70 G71 I72 S73 K74 K75 T76 R77 I78 A79 G80 V81 P85 L90 V91 R92 T93 N94 T95 L96 T97 I101 V102 Q103 I104 D105 A106 T107 P108 F109 R110 E114 G118 Q119 T120 L121 G122 K123 LYS LYS ASN VAL LYS GLU GLU GLU THR VAL ALA LYS SER LYS ASN ALA                      GLU ARG LYS TRP ALA ALA ARG ALA ALA SER ALA LYS ILE E153 S154 S155 Q159 F160 S161 A162 G163 R164 L165 S170 S171 R172 P173 G174 Q175 S176 G177 D180 G181 L184 E185 Y192 L196 T197 A198 K199 K200

• Molecule 41: 40S ribosomal protein S9-A

Chain SK: Page 43 Full wwPDB EM Validation Report EMD-6695, 5WYJ                    MET PRO ARG ALA PRO ARG THR TYR SER LYS THR Y12 S13 T14 P15 K16 R17 E20 R23 A31 G32 E33 F34 G35 N38 E41 I42 Q48 R53 A56 R57 D58 L59 L60 T61 K65 D66 P67 L70 F71 E72 G73 N74 R78 V81 R82 V83 G84                            K91 K92 L93 D94 Y95 V96 L97 A98 L99 K100 F104 L105 E106 R107 Y114 G117 L118 A119 K120 S121 V122 H123 H124 A125 R126 H133 I134 K138 V141 N142 I143 F146 M147 V148 R149 L150 K154 P163 G166 A167 G170 R171 V172 R175 A181 E182    A183 S184 G185 E186 ALA ALA ASP GLU ALA ASP GLU ALA ASP GLU GLU

• Molecule 42: 40S ribosomal protein S11-A

Chain SM:                                         MET S2 T3 E4 L5 T6 V7 E10 R11 A12 P17 H18 I19 F20 N21 N22 P23 K24 V25 K26 T27 S28 K29 R30 T31 K32 R33 K36 L40 G41 F42 K43 T44 E50 G51 S52 Y53 I54 D55 K56 P59 F60 T61 G62 L63 V64 S65 I66 R67 G68 K69 I70 L71 T72                             G73 T74 V75 V76 S77 T78 K79 M80 H81 R82 T83 I84 V85 I86 R87 R88 H92 Y93 I94 Y97 N98 R99 H104 K105 N106 V107 P108 V109 H110 V111 S112 P113 A114 F115 R116 V117 Q118 V119 G120 D121 I122 V123 Q127 C128 R129 P130 I131 S132 K133 T134 V135 R136 F137 N138 K141  V142 SER ALA ALA ALA GLY LYS ALA ASN LYS GLN PHE ALA LYS PHE

• Molecule 43: 40S ribosomal protein S12

Chain SN:                                     MET SER ASP VAL GLU GLU VAL VAL GLU VAL GLN GLU GLU THR VAL VAL GLU GLN THR A20 E21 V22 T23 I24 E25 D26 A27 L28 K29 V30 V31 L32 R33 T34 A35 L36 V37 H38 D39 G40 L41 A42 R43 G44 L45 R46 E47 S48 T49 K50 A51 L52 T53 R54 G55 E56 A57 L58 L59 V60                                                         V61 L62 V63 S64 S65 V66 T67 E68 A69 N70 I71 I72 K73 L74 V75 E76 G77 L78 A79 N80 D81 P82 E83 N84 K85 V86 P87 L88 I89 K90 V91 A92 D93 A94 K95 Q96 L97 G98 E99 W100 A101 G102 L103 G104 K105 I106 D107 R108 E109 G110 N111 A112 R113 K114 V115 V116 G117 A118 S119 V120                        V121 V122 V123 K124 N125 W126 G127 A128 E129 T130 D131 E132 L133 S134 M135 I136 M137 E138 H139 F140 S141 Q142 Q143

• Molecule 44: 40S ribosomal protein S13

Chain SO:                           MET GLY ARG MET HIS SER ALA GLY K9 G10 I11 S12 S13 S14 A15 I16 P17 Y18 S19 R20 N21 A22 L28 I34 I37 Y40 A41 R42 K43 G44 L45 R55 G59 V60 T61 Q62 A63 R64 V65 I66 T67 G68 N69 K70 I71 M72 R73 I74 G79 E83 I84 P85 Page 44 Full wwPDB EM Validation Report EMD-6695, 5WYJ                 E86 D87 L88 L91 H101 R104 N105 R106 K107 D108 K109 D110 A111 R114 L117 I118 E119 S120 R121 I122 H123 R124 L125 A126 R127 T131 V134 P137 Y141 E142 SER ALA THR ALA SER ALA LEU VAL ASN

• Molecule 45: 40S ribosomal protein S14-A

Chain SP:                                  MET SER ASN VAL VAL GLN ALA ARG ASP ASN S11 A17 R18 I19 Y20 A21 S22 F23 T26 F27 V28 H29 V30 T31 D32 L33 S34 G35 K36 E37 T38 R41 V42 T43 G44 G45 V48 K49 A50 D53 E54 V67 I76 T77 I83 R84 A85 T86 G87 G88 T91            K92 L102 R103 A104 L105 A106 R107 S108 R111 I112 G113 R114 I115 E116 D117 V118 T119 PRO VAL PRO SER ASP SER THR ARG LYS LYS GLY GLY ARG ARG GLY ARG ARG LEU

• Molecule 46: 40S ribosomal protein S16-A

Chain SR:                   MET SER A3 V4 P5 S6 V7 F10 G11 K12 K13 K14 H21 V22 K23 A24 G25 K26 I29 G33 I36 V39 E40 P41 E42 I43 L44 R45 F46 K47 V48 Y49 E50 P51 L52 L53 L54 V55 G56 L57 D58 K59 F60 I63 D64 I65 R66 V67 R68 V69 T70 G71 G72           G73 H74 V75 S76 Y79 R82 Q94 E99 K106 T110 S111 Y112 D113 R114 T115 L116 L117 I118 A119 D120 S121 R122 K127 LYS PHE GLY GLY LYS GLY ALA ARG SER ARG PHE GLN LYS SER TYR ARG

• Molecule 47: 40S ribosomal protein S22-A

Chain SX:                      MET T2 R3 L11 N12 A13 I14 N15 N16 A17 E18 K19 T20 G21 K22 R23 L26 I27 R28 S31 I34 G48 E49 F50 E51 Y52 I53 H56 R57 S58 L65 N66 G67 R68 L69 N70 K71 C72 I75 S76 P77 R78 V81 G84 D85 I86 W89 T90 A91                  N92 L93 L94 P95 A96 R97 Q98 F99 G100 Y101 V102 I103 S107 A108 G109 I110 M111 D112 R117 R118 K119 H120 V121 S122 L126 G127 F128 V129 Y130

• Molecule 48: 40S ribosomal protein S23-A

Chain SY:          MET GLY LYS GLY LYS PRO ARG GLY LEU ASN SER ALA ARG LYS LEU ARG VAL HIS ARG ARG ASN ASN ARG TRP ALA GLU ASN ASN TYR LYS LYS ARG LEU LEU GLY THR ALA PHE LYS SER SER PRO F43 H48 A49 K50 G51 L54 E55 K56 K62 Q63 S66 A67 I68 R69                           K70 Q75 L76 I77 K78 N79 G80 K81 K82 A85 F86 V87 P88 N89 D90 N94 F95 V96 D97 D100 E101 V102 L103 F107 G108 R109 K110 G111 K112 A113 K114 G115 D116 I117 P118 G119 V120 R121 F122 K123 K126 V127 S128 G129 V130 S131 L132 E138 K139 K140 E141 K142 P143 Page 45 Full wwPDB EM Validation Report EMD-6695, 5WYJ  R144 S145

• Molecule 49: 40S ribosomal protein S24-A

Chain SZ:                           MET S2 D3 A4 V5 T6 I7 R8 T9 R10 P16 L17 V24 V25 D26 H29 R32 A33 N34 V35 S36 K37 L44 A45 Y48 K49 A50 E51 K52 D53 A54 V55 S56 V57 F58 T62 Q63 F64 F72 V75 Y76 N77 S78 V79 K83 K84 F85 V92        L96 A97 E98 K99 V100 E101 K102 ALA SER ARG GLN GLN ARG LYS GLN LYS LYS ASN ARG ASP LYS LYS ILE PHE GLY THR GLY LYS ARG LEU ALA LYS LYS VAL ALA ARG ARG ASN ALA ASP

• Molecule 50: 40S ribosomal protein S27-A

Chain Sc:                                     MET VAL LEU V4 Q5 D6 H9 P10 T11 A12 E15 L21 Q26 G27 S30 V35 K36 C37 L41 N42 I43 T44 T45 V46 H49 A50 Q51 E57 S58 C59 S60 T61 I62 L63 C64 T67 G68 G69 L73 K82

• Molecule 51: 40S ribosomal protein S28-A

Chain Sd:                     MET ASP ASN LYS T5 V12 I13 K14 V15 L16 G17 R18 T19 G20 S21 D35 T36 S37 K45 G46 P47 V48 R49 D52 I53 L54 V55 L56 M57 E58 S59 R64 R67

• Molecule 52: 40S ribosomal protein S30-A

Chain Sf:  MET ALA LYS VAL HIS GLY SER LEU ALA ARG ALA GLY LYS VAL LYS SER GLN THR PRO LYS VAL GLU K23 R43 G52 LYS ARG ARG MET ASN PRO GLY PRO SER VAL GLN

• Molecule 53: Ubiquitin-40S ribosomal protein S31

Chain Sg: MET GLN ILE PHE VAL LYS THR LEU THR GLY LYS THR ILE THR LEU GLU VAL GLU SER SER ASP THR ILE ASP ASN VAL LYS SER LYS ILE GLN ASP LYS GLU GLY ILE PRO PRO ASP GLN GLN ARG LEU ILE PHE ALA GLY LYS GLN LEU GLU ASP GLY ARG THR LEU SER ASP TYR ASN                    ILE GLN LYS GLU SER THR LEU HIS LEU VAL LEU ARG LEU ARG GLY GLY GLY LYS LYS ARG LYS LYS LYS VAL TYR THR THR PRO LYS LYS ILE LYS HIS LYS HIS LYS LYS VAL LYS LEU ALA V102 L103 S104 Y105 Y106 K107 V108 D109 A110 E111 G112 K113 V114 T115 K116 L117 R118 R119 E120 Page 46 Full wwPDB EM Validation Report EMD-6695, 5WYJ                                 C121 S122 N123 P124 T125 C126 G127 A128 G129 V130 F131 L132 A133 N134 H135 K136 D137 R138 L139 Y140 C141 G142 K143 C144 H145 S146 V147 Y148 K149 V150 N151 A152

• Molecule 54: Utp7

Chain U1:                X1 X5 X13 X14 X18 X19 X22 X23 X26 X27 X28 X29 X30 X31 X34 X35 X36 X47 X48 X49 X50 X57 X61 X66 X77 X88 X94 X95 X96 X99 X100 X105 X109 X110 X111 X112 X113 X114 X115 X116 X117 X118 X119                  X129 X130 X131 X138 X150 X151 X157 X158 X159 X160 X161 X162 X171 X172 X175 X183 X184 X192 X193 X197 X200 X201 X202 X203 X207 X208 X211 X215 X218 X222 X236 X237 X255 X260 X261 X265 X269 X270 X271 X272 X284 X285 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 55: Utp11

Chain U2:   UNK X2 X12 X13 X14 X74 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 56: Utp20

Chain U3:        UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X13 X19 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X31 X32 X40 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X52 X60 X71 X72 X73 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X85 Page 47 Full wwPDB EM Validation Report EMD-6695, 5WYJ         X86 X97 X100 X101 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X113 X120 X121 X131 X132 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X144 X162 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK             UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X195 X196 X197 X202 X203 X204 X205 X210 X211 X212 X213 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X225 X232 X240 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X252 X257 X266 X269 UNK UNK UNK              UNK UNK UNK UNK UNK UNK UNK UNK X281 X293 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X305 X317 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X329 X330 X331 X332 X336 X337 X338 X339 X344 X345 X346 X347 X348 X349 UNK UNK UNK UNK UNK UNK UNK UNK          UNK UNK UNK X361 X362 X363 X371 X372 X373 X374 X375 X376 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X388 X395 X398 X401 X402 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X414 X419 X428 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X440            X449 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X461 X464 X473 X474 X478 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X490 X491 X492 X493 X496 X501 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X513 X519 X520 X521 UNK UNK UNK UNK            UNK UNK UNK UNK UNK UNK UNK X533 X545 X546 X547 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X559 X560 X569 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X581 X582 X585 X586 X593 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X605 X606 X620        X621 X622 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X634 X637 X649 X650 X651 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X665 X666 X678 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X690 X691 X700 X701 UNK UNK UNK UNK UNK UNK UNK        UNK UNK UNK UNK X713 X718 X719 X720 X721 X722 X729 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X741 X755 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X767 X770 X771 X780 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X792 X801 UNK UNK    UNK UNK UNK UNK UNK UNK UNK UNK UNK X813 X825 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X837 X846 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X858 X873 X877 X878 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X890 X900 UNK UNK UNK       UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X920 X921 X922 X923 X924 X925 X931 X932 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X960 X971 UNK UNK UNK UNK UNK      UNK UNK UNK UNK UNK UNK X983 X984 X999 X1000 X1001 X1004 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1015 X1031 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1043 X1056 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK        UNK UNK X1078 X1093 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1105 X1106 X1107 X1108 X1109 X1110 X1116 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1132 X1133 X1134 X1142 X1143 X1144 X1145 X1146 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1158              X1169 X1174 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1186 X1191 X1195 X1196 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1208 X1209 X1210 X1211 X1212 X1213 X1218 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1230 X1234 X1235 X1236 X1237 X1243 UNK Page 48 Full wwPDB EM Validation Report EMD-6695, 5WYJ               UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1255 X1265 X1266 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1278 X1281 X1285 X1286 X1287 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1299 X1300 X1303 X1304 X1307 X1308 X1309 X1310 X1311 X1316 UNK UNK UNK X1320               X1324 X1325 X1329 UNK UNK UNK UNK UNK X1335 X1339 X1340 X1341 X1342 X1343 X1344 UNK UNK UNK UNK UNK UNK UNK UNK X1353 X1374 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1386 X1387 X1388 X1391 X1392 X1396 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1408             X1411 X1412 X1416 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1428 X1429 X1430 X1431 X1432 X1433 X1434 X1443 X1444 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1458 X1461 X1472 X1473 X1476 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1488 X1489               X1490 X1506 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1518 X1521 X1526 X1527 X1528 X1529 X1530 X1539 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1551 X1566 X1571 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1583 X1584 X1591 X1592 X1607 UNK           UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1619 X1634 X1635 X1636 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1648 X1656 X1657 X1666 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1678 X1679 X1680 X1681 X1686 X1687 X1690 X1695 X1696 UNK UNK UNK UNK UNK         UNK UNK UNK UNK UNK UNK X1708 X1720 X1721 X1722 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1734 X1748 X1749 X1750 X1751 X1752 X1753 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1765 X1766 X1767 X1782 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1794 X1805             X1811 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1823 X1824 X1827 X1828 X1829 X1842 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1854 X1860 X1861 X1862 X1872 X1876 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1888 X1901 X1902 X1903 X1904 UNK UNK          UNK UNK UNK UNK UNK UNK UNK UNK UNK X1916 X1931 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1943 X1944 X1945 X1946 X1947 X1951 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1963 X1968 X1969 X1970 X1979 X1980 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK           X1992 X2007 X2008 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2020 X2029 X2036 X2037 X2038 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2050 X2051 X2066 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2078 X2079 X2089 X2090 X2094 UNK UNK UNK UNK UNK       UNK UNK UNK UNK UNK UNK X2106 X2111 X2112 X2113 X2124 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2136 X2137 X2138 X2147 X2151 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2163 X2182 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2194 X2209 UNK       UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2221 X2222 X2223 X2234 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2246 X2267 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2279 X2289 X2299 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2311 X2328 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2340 X2355 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2367 X2379 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2391 X2406 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X2418 X2432 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK

• Molecule 57: rRNA-processing protein FCF1 Page 49 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain U4:   MET GLY LYS ALA LYS LYS THR ARG LYS PHE GLY LEU VAL LYS ARG THR LEU ASN THR LYS LYS ASP GLN ARG LEU LYS LYS ASN GLN GLU ASN ILE LYS THR LYS GLU ASP PRO GLU LEU THR ARG ASN ILE PRO GLN VAL SER SER ALA LEU PHE PHE GLN TYR ASN Q57 A58 I59 K60                     Y63 Q64 V65 L66 I67 D68 T69 N70 F71 I72 N73 I76 K79 V80 D81 I82 V83 R84 D88 L91 A92 K93 C94 N95 P96 L97 D100 C101 L106 R114 I115 A116 L117 K118 R121 R124 I125 A137 L141 R144 K149 C150 Y151 I152 T155            N156 D157 L160 R163 I164 G169 I170 V175 G176 A179 I182 GLU LYS LEU PRO ASP VAL PHE

• Molecule 58: Ribosome biogenesis protein UTP30

Chain U5:                                                 MET VAL GLU SER ASN ASP ILE I8 K9 S10 G11 L12 A13 E14 K15 A16 L17 K18 A19 L20 I21 L22 Q23 C24 E25 E26 N27 P28 S29 L30 K31 N32 D33 K34 D35 I36 H37 I38 I39 I40 N41 T42 G43 K44 K45 M46 G47 I48 N49 R50 D51 N52 I53 P54 R55 I56 I57 P58 L59 T60                                                     K61 Y62 K63 L64 F65 K66 P67 R68 D69 L70 N71 I72 L73 L74 I75 T76 K77 D78 P79 S80 A81 L82 Y83 R84 E85 T86 L87 T88 K89 D90 E91 H92 T93 S94 E95 L96 F97 K98 E99 I100 I101 S102 V103 K104 N105 L106 R109 F110 K111 G112 S113 K114 L115 T116 Q117 L118 Y119 F122                                                    D123 L124 V125 V126 A127 D128 Y129 R130 V131 H132 H133 L134 L135 P136 E137 V138 L139 G140 S141 R142 F143 Y144 H145 G146 S147 K148 K149 L150 P151 Y152 M153 I154 R155 M156 S157 K158 E159 V160 K161 L162 K163 R164 Q165 Q166 M167 V168 E169 K170 C171 D172 P173 I174 Y175 V176 R177 L180 R181 S182 I183                                                      C184 K185 N186 T187 S188 Y189 I190 P191 N192 N193 D194 N195 C196 L197 S198 V199 R200 V201 G202 Y203 I204 Q205 K206 H207 S208 I209 I212 L213 Q214 N215 I216 Q217 D218 T219 I220 N221 F222 L223 T224 D225 K226 S227 K228 R229 P230 Q231 G232 G233 V234 I235 K236 G237 G238 I239 I240 S241 I242 F243 V244          K245 T246 S247 N248 S249 T250 S251 L252 P253 I254 Y255 GLN PHE SER GLU ALA ARG GLU ASN GLN LYS ASN GLU ASP LEU SER ASP ILE LYS LEU

• Molecule 59: Helical domain protein

Chain UA: UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X101 X121 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X202 X225 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK Page 50 Full wwPDB EM Validation Report EMD-6695, 5WYJ UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X303 X331 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X401 X426 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X501 X517 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X601 X617 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X702 X715 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X801 X816 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X904 X932 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK   UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1001 X1005 X1006 X1028 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1106 X1131 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1201 X1222 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1306 X1325 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1401 X1418 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK Page 51 Full wwPDB EM Validation Report EMD-6695, 5WYJ    UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X1504 X1505 X1506 X1521 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK  UNK UNK UNK UNK UNK UNK UNK X1603 X1608 X1615

• Molecule 60: Helical domain protein

Chain UB:             UNK UNK UNK X4 X5 X6 X7 X13 UNK UNK X16 X19 X20 X21 X22 X23 X34 X35 X36 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X54 X78 X79 X80 UNK UNK UNK UNK UNK UNK UNK X88 X97 X98 X99 UNK UNK UNK UNK     UNK UNK X106 X114 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X156 X172 UNK UNK UNK UNK UNK X178 X179 X180 X181 X194          X195 X196 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X207 X221 X222 X223 UNK UNK UNK UNK UNK X229 X243 X247 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X260 X276 UNK UNK UNK UNK X281 X282 X283 X290 X299 UNK UNK UNK UNK UNK UNK UNK      UNK UNK UNK X310 X318 X326 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X337 X350 UNK UNK UNK UNK UNK UNK UNK UNK UNK X360 X371 X372 X373 X377 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X394 X410 UNK UNK UNK UNK       UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X433 X434 X446 UNK UNK UNK UNK UNK UNK UNK UNK UNK X456 X457 X458 X459 X464 X465 X473 UNK UNK UNK UNK UNK X479 X492 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X503           X511 X516 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X530 X531 X532 X546 X547 X551 UNK UNK UNK UNK UNK UNK UNK UNK X560 X584 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X600 X614 X615 X616 X623 UNK UNK UNK      UNK UNK UNK UNK UNK UNK X633 X642 UNK UNK X645 X654 X661 UNK UNK UNK X665 X689 UNK UNK X692 X696 X705 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X727 X743 X744 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X758 X773 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X815 X826 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X918 X934 X938 UNK UNK UNK UNK UNK UNK UNK UNK X947 X962 UNK UNK UNK X966 X987

• Molecule 61: Unassigned helices Page 52 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Chain UC:            X1 X9 X20 UNK UNK UNK X24 X25 X26 X43 UNK UNK UNK UNK UNK UNK UNK X51 X52 X53 X54 X55 X56 X57 X58 X62 X63 X64 X69 X73 X76 X77 X78 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK   UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X140 X171 UNK X173 X179 X180 X202 UNK UNK UNK UNK UNK UNK UNK UNK UNK     X212 X233 X243 X247 X250 X271 UNK UNK UNK X275 X297 X301 UNK UNK UNK X305 X306 X310 X323 X324 UNK UNK UNK UNK UNK X330 X365 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X380 X399 UNK UNK UNK UNK X404   X431 UNK UNK UNK UNK X436 X437 X441 X445 X449 X450 X451 UNK UNK UNK UNK X456 X470 UNK UNK UNK UNK X475 X494 UNK UNK UNK UNK X499 X500 X501 X515 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK      UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X696 X697 X702 X706 X728 X729 X730 X731 X732 X733 X734 X752 UNK UNK UNK UNK UNK UNK                    UNK UNK UNK UNK X763 X769 X770 X771 X772 X773 X774 X775 X779 UNK UNK UNK UNK UNK X785 X786 X787 X788 X789 X790 X791 X792 X793 X796 X797 X798 X799 X807 X808 X809 X810 UNK UNK X813 X814 X815 X816 X817 X824 X825 UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK                            UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK X850 X872 UNK UNK UNK UNK UNK UNK UNK UNK X881 X882 X883 X884 X885 X888 X889 X890 X893 X894 X895 X896 X897 X898 X899 X900 X901 X902 X903 X904 X905 X906 X907 X908 X909 X910 X911 X912 X913 X914 UNK UNK UNK UNK             UNK UNK X921 X922 X923 X927 X942 UNK UNK UNK UNK UNK UNK UNK UNK X951 X962 X963 X964 X965 X966 X967 X968 X969 X970 UNK X972 X989 UNK X991 X992 X993 X1008 UNK UNK X1011 X1033 Page 53 Full wwPDB EM Validation Report EMD-6695, 5WYJ

4 Experimental information ○i

Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry POINT, C1 Depositor Number of particles used 30995 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method PHASE FLIPPING ONLY Depositor Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor 2 Electron dose (e−/Å ) 40 Depositor Minimum defocus (nm) 2000 Depositor Maximum defocus (nm) 4000 Depositor Magnification 79545 Depositor Image detector OTHER Depositor Maximum map value 0.342 Depositor Minimum map value -0.265 Depositor Average map value 0.000 Depositor Map value standard deviation 0.018 Depositor Recommended contour level 0.09 Depositor Map size (Å) 704.0, 704.0, 704.0 wwPDB Map dimensions 400, 400, 400 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.76, 1.76, 1.76 Depositor Page 54 Full wwPDB EM Validation Report EMD-6695, 5WYJ

5 Model quality ○i

5.1 Standard geometry ○i

The Z score for a bond length (or angle) is the number of standard deviations the observed value is removed from the expected value. A bond length (or angle) with |Z| > 5 is considered an outlier worth inspection. RMSZ is the root-mean-square of all Z scores of the bond lengths (or angles).

Bond lengths Bond angles Mol Chain RMSZ #|Z| >5 RMSZ #|Z| >5 1 3A 0.34 0/3710 1.04 6/5763 (0.1%) 2 3B 0.31 0/1903 0.53 0/2572 2 3C 0.28 0/1903 0.52 1/2572 (0.0%) 3 3D 0.30 0/2960 0.52 0/3987 4 3E 0.27 0/2970 0.46 0/4004 5 3F 0.30 0/2975 0.56 0/4008 6 3G 0.28 0/936 0.51 0/1273 6 3H 0.27 0/936 0.50 0/1273 7 5A 0.31 0/11029 1.07 51/17170 (0.3%) 12 AE 0.41 1/6194 (0.0%) 0.58 5/8384 (0.1%) 15 B1 0.30 0/4431 0.56 0/5988 16 BA 0.31 0/6164 0.58 4/8349 (0.0%) 17 BB 0.29 0/6264 0.59 0/8473 18 BC 0.29 0/6226 0.60 2/8442 (0.0%) 19 BD 0.46 4/2597 (0.2%) 0.64 4/3520 (0.1%) 20 BE 0.30 0/6056 0.56 0/8189 21 CA 0.27 0/1621 0.45 0/2196 22 CB 0.28 0/9081 0.47 0/12283 23 E1 0.25 0/1716 0.47 0/2319 23 E2 0.26 0/1721 0.48 0/2323 24 E3 0.29 0/2168 0.51 1/2942 (0.0%) 26 K1 0.29 0/1432 0.53 1/1926 (0.1%) 27 MA 0.27 0/1117 0.47 0/1509 28 MB 0.30 0/1496 0.53 0/2025 29 MC 0.26 0/209 0.50 0/282 30 P1 0.27 0/1394 0.51 0/1881 31 R1 0.28 0/2793 0.49 0/3774 32 R2 0.26 0/2271 0.44 0/3054 34 SA 0.70 9/26553 (0.0%) 1.17 165/41320 (0.4%) 35 SC 0.31 0/1735 0.72 1/2335 (0.0%) 36 SF 0.30 0/1920 0.64 3/2589 (0.1%) 37 SG 0.34 0/1629 0.62 0/2202 38 SH 0.28 0/1385 0.58 1/1851 (0.1%) 39 SI 0.33 0/1343 0.64 1/1808 (0.1%) Page 55 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Bond lengths Bond angles Mol Chain RMSZ #|Z| >5 RMSZ #|Z| >5 40 SJ 0.28 0/1373 0.60 1/1834 (0.1%) 41 SK 0.29 0/1434 0.62 1/1920 (0.1%) 42 SM 0.35 0/1169 0.57 0/1576 43 SN 0.31 0/898 0.79 1/1220 (0.1%) 44 SO 0.28 0/1109 0.54 0/1495 45 SP 0.28 0/758 0.58 0/1028 46 SR 0.31 0/990 0.66 0/1335 47 SX 0.28 0/1038 0.58 0/1395 48 SY 0.30 0/796 0.67 0/1062 49 SZ 0.31 0/814 0.53 0/1093 50 Sc 0.28 0/605 0.56 0/817 51 Sd 0.26 0/499 0.55 0/670 52 Sf 0.29 0/255 0.54 0/339 53 Sg 0.29 0/404 0.56 0/542 57 U4 0.28 0/1007 0.54 0/1357 58 U5 0.27 0/2043 0.52 0/2747 All All 0.41 14/144030 (0.0%) 0.78 249/203016 (0.1%)

Chiral center outliers are detected by calculating the chiral volume of a chiral center and verifying if the center is modelled as a planar moiety or with the opposite hand.A planarity outlier is detected by checking planarity of atoms in a peptide group, atoms in a mainchain group or atoms of a sidechain that are expected to be planar.

Mol Chain #Chirality outliers #Planarity outliers 3 3D 0 2 5 3F 0 2 12 AE 0 1 15 B1 0 3 16 BA 0 2 17 BB 0 7 18 BC 0 6 19 BD 0 3 20 BE 0 4 21 CA 0 1 22 CB 0 1 34 SA 0 1 35 SC 0 2 36 SF 0 2 37 SG 0 7 38 SH 0 1 39 SI 0 3 41 SK 0 3 Continued on next page... Page 56 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain #Chirality outliers #Planarity outliers 42 SM 0 1 43 SN 0 4 45 SP 0 1 46 SR 0 3 48 SY 0 1 53 Sg 0 1 58 U5 0 1 61 UC 0 2 All All 0 65

All (14) bond length outliers are listed below:

Mol Chain Res Type Atoms Z Observed(Å) Ideal(Å) 34 SA 283 U C2-N3 53.18 1.75 1.37 34 SA 283 U N3-C4 40.53 1.75 1.38 34 SA 283 U N1-C2 39.84 1.74 1.38 34 SA 283 U N1-C6 36.49 1.70 1.38 34 SA 283 U C4-C5 32.43 1.72 1.43 34 SA 283 U C5-C6 29.26 1.60 1.34 12 AE 571 LEU CA-C 22.36 2.11 1.52 34 SA 493 U C2-N3 -14.56 1.27 1.37 19 BD 411 TYR CB-CG -10.84 1.35 1.51 19 BD 411 TYR CE1-CZ 8.68 1.49 1.38 34 SA 487 G C5-C6 7.07 1.49 1.42 19 BD 411 TYR CD1-CE1 -6.50 1.29 1.39 34 SA 487 G C5-C4 6.39 1.42 1.38 19 BD 411 TYR CD2-CE2 -5.22 1.31 1.39

All (249) bond angle outliers are listed below:

Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 487 G C5-C6-O6 38.33 151.60 128.60 34 SA 487 G N1-C6-O6 -33.63 99.72 119.90 34 SA 493 U C5-C4-O4 25.11 140.96 125.90 34 SA 493 U N3-C4-O4 -20.41 105.11 119.40 34 SA 487 G C4-C5-N7 -19.97 102.81 110.80 34 SA 493 U C2-N3-C4 16.32 136.79 127.00 12 AE 571 LEU O-C-N -15.12 98.51 122.70 34 SA 493 U N1-C2-N3 -14.92 105.95 114.90 34 SA 487 G C5-N7-C8 13.80 111.20 104.30 12 AE 571 LEU CB-CA-C 13.02 134.94 110.20 34 SA 487 G C5-C6-N1 -11.74 105.63 111.50 Continued on next page... Page 57 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 283 U C6-N1-C2 11.51 127.91 121.00 34 SA 487 G C6-C5-N7 10.96 136.98 130.40 34 SA 283 U N1-C2-N3 -10.37 108.68 114.90 34 SA 1082 C C2-N1-C1’ 10.24 130.07 118.80 7 5A 522 C N3-C2-O2 -9.81 115.03 121.90 34 SA 1537 C N1-C2-O2 9.78 124.77 118.90 34 SA 487 G C6-N1-C2 9.59 130.85 125.10 12 AE 571 LEU N-CA-CB -9.52 91.37 110.40 34 SA 493 U C4-C5-C6 -9.50 114.00 119.70 34 SA 558 U N3-C2-O2 -9.19 115.77 122.20 34 SA 1537 C C2-N1-C1’ 9.02 128.72 118.80 34 SA 1456 C N1-C2-O2 8.96 124.28 118.90 7 5A 356 C N1-C2-O2 8.94 124.26 118.90 34 SA 1456 C C2-N1-C1’ 8.84 128.52 118.80 7 5A 355 C N1-C2-O2 8.62 124.07 118.90 39 SI 87 ASP CB-CG-OD1 8.56 126.00 118.30 34 SA 1159 C C2-N1-C1’ 8.48 128.13 118.80 7 5A 522 C N1-C2-O2 8.29 123.88 118.90 34 SA 1537 C N3-C2-O2 -8.20 116.16 121.90 12 AE 571 LEU CA-C-N 8.12 135.06 117.20 7 5A 356 C C6-N1-C2 -7.95 117.12 120.30 12 AE 571 LEU CA-CB-CG 7.93 133.55 115.30 34 SA 1082 C C6-N1-C1’ -7.93 111.28 120.80 34 SA 1456 C N3-C2-O2 -7.89 116.37 121.90 34 SA 1159 C N1-C2-O2 7.83 123.60 118.90 34 SA 487 G N9-C4-C5 7.80 108.52 105.40 34 SA 1624 C N1-C2-O2 7.76 123.56 118.90 34 SA 184 C N1-C2-O2 7.63 123.48 118.90 34 SA 107 C N1-C2-O2 7.50 123.40 118.90 34 SA 1246 C C2-N1-C1’ 7.49 127.04 118.80 34 SA 394 C N1-C2-O2 7.40 123.34 118.90 7 5A 356 C N3-C2-O2 -7.37 116.74 121.90 34 SA 1082 C N1-C2-O2 7.34 123.30 118.90 34 SA 1258 U C2-N1-C1’ 7.24 126.39 117.70 19 BD 411 TYR CB-CG-CD1 -7.17 116.70 121.00 34 SA 1596 C N1-C2-O2 7.14 123.19 118.90 7 5A 355 C N3-C2-O2 -7.12 116.91 121.90 34 SA 1537 C C6-N1-C2 -7.11 117.46 120.30 19 BD 423 LEU CA-CB-CG 7.08 131.59 115.30 7 5A 432 C N1-C2-O2 7.07 123.14 118.90 34 SA 1246 C N1-C2-O2 7.02 123.11 118.90 7 5A 469 C N1-C2-O2 7.02 123.11 118.90 Continued on next page... Page 58 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 1258 U N1-C2-O2 7.01 127.71 122.80 34 SA 934 C N1-C2-O2 6.95 123.07 118.90 34 SA 1159 C N3-C2-O2 -6.86 117.09 121.90 34 SA 1220 C C2-N1-C1’ 6.86 126.35 118.80 19 BD 411 TYR CA-CB-CG -6.82 100.44 113.40 34 SA 1258 U N3-C2-O2 -6.82 117.43 122.20 34 SA 283 U N3-C4-C5 6.79 118.68 114.60 34 SA 186 C C2-N1-C1’ 6.76 126.24 118.80 34 SA 185 U C2-N1-C1’ 6.75 125.80 117.70 34 SA 531 C N1-C2-O2 6.75 122.95 118.90 7 5A 69 U N1-C2-O2 6.72 127.51 122.80 34 SA 107 C N3-C2-O2 -6.67 117.23 121.90 7 5A 69 U C2-N1-C1’ 6.67 125.70 117.70 34 SA 1596 C C2-N1-C1’ 6.64 126.11 118.80 7 5A 432 C C2-N1-C1’ 6.63 126.09 118.80 34 SA 394 C N3-C2-O2 -6.61 117.28 121.90 34 SA 1220 C C6-N1-C2 -6.59 117.66 120.30 34 SA 185 U N1-C2-O2 6.53 127.37 122.80 34 SA 1456 C C6-N1-C2 -6.50 117.70 120.30 7 5A 522 C C6-N1-C2 -6.50 117.70 120.30 1 3A 38 U C2-N1-C1’ 6.49 125.48 117.70 34 SA 405 C C5-C6-N1 6.49 124.25 121.00 34 SA 1473 U N3-C2-O2 -6.48 117.66 122.20 34 SA 1072 C C5-C6-N1 6.46 124.23 121.00 34 SA 1251 U O5’-P-OP1 -6.43 99.91 105.70 7 5A 356 C C5-C6-N1 6.43 124.21 121.00 34 SA 1624 C C2-N1-C1’ 6.41 125.85 118.80 34 SA 934 C C2-N1-C1’ 6.40 125.84 118.80 34 SA 1063 U N1-C2-O2 6.39 127.27 122.80 35 SC 61 LEU CA-CB-CG 6.38 129.97 115.30 34 SA 1220 C C5-C6-N1 6.33 124.16 121.00 34 SA 1063 U N3-C2-O2 -6.25 117.82 122.20 7 5A 342 C N1-C2-O2 6.25 122.65 118.90 1 3A 38 U N1-C2-O2 6.24 127.17 122.80 7 5A 433 C N1-C2-O2 6.22 122.63 118.90 34 SA 1658 G N3-C4-N9 6.21 129.73 126.00 34 SA 136 C C2-N1-C1’ 6.20 125.62 118.80 7 5A 508 C C2-N1-C1’ 6.18 125.60 118.80 34 SA 184 C C2-N1-C1’ 6.18 125.60 118.80 34 SA 185 U N3-C2-O2 -6.17 117.89 122.20 34 SA 908 U N1-C2-O2 6.16 127.11 122.80 1 3A 38 U N3-C2-O2 -6.16 117.89 122.20 Continued on next page... Page 59 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 1624 C N3-C2-O2 -6.16 117.59 121.90 34 SA 1159 C C6-N1-C2 -6.15 117.84 120.30 18 BC 133 ASN C-N-CA 6.13 135.18 122.30 7 5A 69 U N3-C2-O2 -6.12 117.92 122.20 7 5A 547 C N1-C2-O2 6.11 122.56 118.90 7 5A 441 C C2-N1-C1’ 6.10 125.51 118.80 34 SA 1473 U N1-C2-O2 6.09 127.06 122.80 34 SA 531 C C6-N1-C2 -6.08 117.87 120.30 34 SA 192 U P-O3’-C3’ 6.06 126.97 119.70 7 5A 433 C C2-N1-C1’ 6.06 125.46 118.80 34 SA 25 C P-O3’-C3’ 6.04 126.95 119.70 34 SA 250 C C2-N1-C1’ 6.03 125.43 118.80 7 5A 441 C N1-C2-O2 6.00 122.50 118.90 34 SA 1624 C C6-N1-C2 -5.97 117.91 120.30 34 SA 1191 U C2-N1-C1’ 5.94 124.82 117.70 34 SA 1248 C C2-N1-C1’ 5.90 125.29 118.80 34 SA 1082 C O4’-C1’-N1 5.89 112.91 108.20 1 3A 304 U C2-N1-C1’ 5.88 124.76 117.70 34 SA 440 U C2-N1-C1’ 5.87 124.75 117.70 34 SA 908 U N3-C2-O2 -5.86 118.10 122.20 34 SA 1456 C C6-N1-C1’ -5.86 113.77 120.80 34 SA 487 G N3-C4-N9 -5.86 122.48 126.00 34 SA 1536 G N3-C4-C5 -5.85 125.67 128.60 36 SF 192 ILE CG1-CB-CG2 -5.85 98.53 111.40 34 SA 531 C C2-N1-C1’ 5.84 125.23 118.80 34 SA 1537 C C6-N1-C1’ -5.82 113.82 120.80 34 SA 1248 C C6-N1-C2 -5.80 117.98 120.30 34 SA 1174 C C5-C6-N1 5.79 123.90 121.00 34 SA 184 C N3-C2-O2 -5.78 117.85 121.90 34 SA 249 U C2-N1-C1’ 5.78 124.64 117.70 34 SA 1620 C N1-C2-O2 5.78 122.37 118.90 34 SA 531 C C5-C6-N1 5.78 123.89 121.00 34 SA 1246 C N3-C2-O2 -5.78 117.86 121.90 34 SA 1536 G C4-N9-C1’ 5.78 134.01 126.50 34 SA 283 U N1-C2-O2 5.77 126.84 122.80 7 5A 115 G N1-C6-O6 -5.77 116.44 119.90 18 BC 136 ILE C-N-CA 5.77 136.12 121.70 34 SA 934 C N3-C2-O2 -5.76 117.87 121.90 7 5A 480 C C2-N1-C1’ 5.75 125.12 118.80 34 SA 1573 A P-O3’-C3’ 5.74 126.59 119.70 34 SA 405 C C6-N1-C2 -5.73 118.01 120.30 2 3C 306 LEU C-N-CA 5.70 135.96 121.70 Continued on next page... Page 60 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 1072 C C2-N1-C1’ 5.70 125.07 118.80 34 SA 965 U N1-C2-O2 5.70 126.79 122.80 34 SA 260 U C2-N1-C1’ 5.69 124.53 117.70 1 3A 49 C N1-C2-O2 5.67 122.30 118.90 7 5A 442 U C2-N1-C1’ 5.67 124.50 117.70 34 SA 1573 A OP2-P-O3’ 5.67 117.66 105.20 41 SK 99 LEU CA-CB-CG 5.66 128.31 115.30 7 5A 508 C C5-C6-N1 5.65 123.82 121.00 34 SA 1159 C C6-N1-C1’ -5.64 114.03 120.80 34 SA 558 U N1-C2-N3 5.64 118.28 114.90 34 SA 1615 C N1-C2-O2 5.63 122.28 118.90 34 SA 440 U N1-C2-O2 5.63 126.74 122.80 7 5A 355 C C6-N1-C2 -5.62 118.05 120.30 34 SA 107 C C6-N1-C2 -5.62 118.05 120.30 34 SA 1246 C C6-N1-C2 -5.61 118.06 120.30 7 5A 356 C C2-N1-C1’ 5.61 124.97 118.80 38 SH 68 LEU CB-CG-CD2 5.61 120.54 111.00 34 SA 543 C N1-C2-O2 5.61 122.27 118.90 34 SA 1473 U C2-N1-C1’ 5.61 124.43 117.70 7 5A 432 C N3-C2-O2 -5.61 117.98 121.90 34 SA 507 U C2-N1-C1’ 5.59 124.41 117.70 34 SA 111 U N1-C2-O2 5.59 126.71 122.80 7 5A 109 C C2-N1-C1’ 5.58 124.94 118.80 34 SA 274 G C8-N9-C1’ -5.57 119.76 127.00 34 SA 1596 C N3-C2-O2 -5.56 118.01 121.90 34 SA 1248 C N1-C2-O2 5.55 122.23 118.90 7 5A 128 C N1-C2-O2 5.53 122.22 118.90 34 SA 1617 U C2-N1-C1’ 5.53 124.33 117.70 7 5A 508 C N1-C2-O2 5.52 122.21 118.90 34 SA 282 C N1-C2-O2 5.51 122.21 118.90 34 SA 1536 G N3-C4-N9 5.51 129.31 126.00 34 SA 1658 G C4-N9-C1’ 5.51 133.66 126.50 34 SA 507 U N1-C2-O2 5.48 126.64 122.80 34 SA 1624 C C5-C6-N1 5.47 123.73 121.00 36 SF 228 ILE CG1-CB-CG2 -5.46 99.38 111.40 34 SA 283 U C5-C6-N1 -5.46 119.97 122.70 34 SA 1257 U C2-N1-C1’ 5.46 124.25 117.70 7 5A 547 C C6-N1-C2 -5.44 118.12 120.30 34 SA 274 G N3-C4-N9 5.43 129.26 126.00 7 5A 186 C C5-C6-N1 5.42 123.71 121.00 34 SA 960 U N1-C2-O2 5.41 126.59 122.80 34 SA 1481 C P-O3’-C3’ 5.41 126.20 119.70 Continued on next page... Page 61 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 16 BA 721 VAL C-N-CA -5.39 108.22 121.70 34 SA 250 C N1-C2-O2 5.39 122.14 118.90 34 SA 507 U N3-C2-O2 -5.39 118.43 122.20 7 5A 385 A P-O3’-C3’ 5.38 126.16 119.70 34 SA 274 G C4-N9-C1’ 5.38 133.50 126.50 34 SA 1510 U N1-C2-O2 5.38 126.56 122.80 34 SA 559 C C2-N1-C1’ 5.38 124.71 118.80 7 5A 245 C C5-C6-N1 5.37 123.69 121.00 34 SA 1066 C N3-C2-O2 -5.37 118.14 121.90 34 SA 965 U N3-C2-O2 -5.36 118.45 122.20 34 SA 1617 U N1-C2-O2 5.34 126.54 122.80 34 SA 1072 C N1-C2-O2 5.33 122.10 118.90 34 SA 285 G C4-N9-C1’ 5.33 133.43 126.50 34 SA 960 U N3-C2-O2 -5.33 118.47 122.20 26 K1 65 ASP CB-CG-OD1 5.31 123.08 118.30 34 SA 1537 C C5-C6-N1 5.31 123.65 121.00 7 5A 261 U N3-C2-O2 -5.30 118.49 122.20 16 BA 546 ASP CB-CG-OD1 5.30 123.07 118.30 34 SA 440 U N3-C2-O2 -5.30 118.49 122.20 16 BA 717 LEU CA-CB-CG 5.29 127.47 115.30 34 SA 531 C N3-C2-O2 -5.27 118.21 121.90 34 SA 1072 C C6-N1-C2 -5.27 118.19 120.30 7 5A 442 U N1-C2-O2 5.26 126.48 122.80 7 5A 442 U N3-C2-O2 -5.26 118.52 122.20 16 BA 521 LEU CA-CB-CG 5.26 127.39 115.30 34 SA 1636 C N1-C2-O2 5.25 122.05 118.90 34 SA 106 U N1-C2-O2 5.25 126.47 122.80 34 SA 1191 U N1-C2-O2 5.25 126.47 122.80 43 SN 89 ILE C-N-CA 5.24 134.80 121.70 7 5A 454 C C2-N1-C1’ 5.22 124.54 118.80 40 SJ 22 ARG CA-CB-CG 5.22 124.88 113.40 34 SA 1527 C C2-N1-C1’ 5.21 124.53 118.80 7 5A 342 C N3-C2-O2 -5.20 118.26 121.90 7 5A 355 C C2-N1-C1’ 5.19 124.51 118.80 34 SA 453 U C2-N1-C1’ 5.19 123.93 117.70 7 5A 186 C C6-N1-C2 -5.19 118.23 120.30 7 5A 261 U N1-C2-O2 5.18 126.43 122.80 19 BD 411 TYR CB-CA-C 5.18 120.75 110.40 34 SA 1596 C C6-N1-C2 -5.17 118.23 120.30 7 5A 469 C N3-C2-O2 -5.16 118.29 121.90 34 SA 1063 U C2-N1-C1’ 5.16 123.89 117.70 34 SA 249 U N1-C2-O2 5.15 126.41 122.80 Continued on next page... Page 62 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Atoms Z Observed(o) Ideal(o) 34 SA 965 U C2-N1-C1’ 5.14 123.87 117.70 24 E3 211 ILE C-N-CA 5.13 134.53 121.70 34 SA 136 C N1-C2-O2 5.12 121.97 118.90 34 SA 638 U C2-N1-C1’ 5.11 123.83 117.70 7 5A 178 G N3-C4-N9 5.08 129.05 126.00 7 5A 469 C C2-N1-C1’ 5.08 124.39 118.80 7 5A 131 C N1-C2-O2 5.07 121.94 118.90 34 SA 260 U N3-C2-O2 -5.07 118.65 122.20 36 SF 180 LEU CA-CB-CG 5.06 126.93 115.30 34 SA 50 C N1-C2-O2 5.05 121.93 118.90 34 SA 1441 C P-O3’-C3’ 5.04 125.75 119.70 7 5A 524 U C5-C6-N1 5.04 125.22 122.70 34 SA 1619 C N1-C2-O2 5.04 121.92 118.90 34 SA 111 U C2-N1-C1’ 5.04 123.75 117.70 7 5A 547 C N3-C2-O2 -5.04 118.37 121.90 34 SA 1082 C N3-C2-O2 -5.03 118.38 121.90 34 SA 1275 A P-O3’-C3’ 5.03 125.73 119.70 34 SA 1510 U N3-C2-O2 -5.03 118.68 122.20 1 3A 304 U N1-C2-O2 5.03 126.32 122.80 34 SA 186 C C5-C6-N1 5.02 123.51 121.00 34 SA 638 U N1-C2-O2 5.02 126.32 122.80 34 SA 1082 C C5-C6-N1 5.02 123.51 121.00 34 SA 107 C C2-N1-C1’ 5.02 124.32 118.80 34 SA 285 G N3-C4-N9 5.01 129.01 126.00 34 SA 1729 C N1-C2-O2 5.01 121.91 118.90 7 5A 547 C C5-C6-N1 5.01 123.51 121.00 34 SA 361 C C6-N1-C2 -5.01 118.30 120.30 34 SA 1209 C C5-C6-N1 5.01 123.50 121.00

There are no chirality outliers. All (65) planarity outliers are listed below:

Mol Chain Res Type Group 3 3D 392 TYR Peptide 3 3D 91 SER Peptide 5 3F 342 ARG Peptide 5 3F 437 ARG Peptide 12 AE 64 PRO Peptide 15 B1 190 ILE Peptide 15 B1 234 PRO Peptide 15 B1 73 THR Peptide 16 BA 515 TYR Peptide Continued on next page... Page 63 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Group 16 BA 702 LEU Peptide 17 BB 169 PHE Peptide 17 BB 235 ASN Peptide 17 BB 335 LEU Peptide 17 BB 795 ILE Peptide 17 BB 796 ALA Peptide 17 BB 797 VAL Peptide 17 BB 864 ASN Peptide 18 BC 133 ASN Peptide 18 BC 136 ILE Peptide 18 BC 299 ASN Peptide 18 BC 365 ILE Peptide 18 BC 771 LYS Peptide 18 BC 772 LEU Peptide 19 BD 304 LEU Peptide 19 BD 411 TYR Sidechain,Peptide 20 BE 660 PHE Peptide 20 BE 665 THR Peptide 20 BE 667 THR Peptide 20 BE 94 TYR Peptide 21 CA 199 GLN Peptide 22 CB 555 PHE Peptide 34 SA 493 U Sidechain 35 SC 206 PRO Peptide 35 SC 208 GLN Peptide 36 SF 193 GLY Peptide 36 SF 194 THR Peptide 37 SG 125 THR Peptide 37 SG 127 GLN Peptide 37 SG 128 ASN Peptide 37 SG 48 PHE Peptide 37 SG 49 GLU Peptide 37 SG 50 GLU Peptide 37 SG 65 ARG Peptide 38 SH 68 LEU Peptide 39 SI 131 PHE Peptide 39 SI 156 SER Peptide 39 SI 64 VAL Peptide 41 SK 117 GLY Peptide 41 SK 133 HIS Peptide 41 SK 163 PRO Peptide 42 SM 3 THR Peptide Continued on next page... Page 64 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type Group 43 SN 105 LYS Peptide 43 SN 110 GLY Peptide 43 SN 130 THR Peptide 43 SN 84 ASN Peptide 45 SP 41 ARG Peptide 46 SR 33 GLY Peptide 46 SR 40 GLU Peptide 46 SR 52 LEU Peptide 48 SY 96 VAL Peptide 53 Sg 147 VAL Peptide 58 U5 62 TYR Peptide 61 UC 233 UNK Mainchain,Peptide

5.2 Too-close contacts ○i

In the following table, the Non-H and H(model) columns list the number of non-hydrogen atoms and hydrogen atoms in the chain respectively. The H(added) column lists the number of hydrogen atoms added and optimized by MolProbity. The Clashes column lists the number of clashes within the asymmetric unit, whereas Symm-Clashes lists symmetry-related clashes.

Mol Chain Non-H H(model) H(added) Clashes Symm-Clashes 1 3A 3327 0 1687 58 0 2 3B 1866 0 1904 30 0 2 3C 1866 0 1904 20 0 3 3D 2915 0 2930 43 0 4 3E 2935 0 3033 48 0 5 3F 2916 0 2947 82 0 6 3G 924 0 975 20 0 6 3H 924 0 975 17 0 7 5A 9867 0 4954 82 0 8 AA 2845 0 658 47 0 9 AB 2015 0 454 19 0 10 AC 2360 0 534 17 0 11 AD 505 0 116 3 0 12 AE 9970 0 7056 161 0 13 AF 1880 0 425 23 0 14 AG 3060 0 707 36 0 15 B1 4325 0 4434 83 0 16 BA 6026 0 5940 129 0 17 BB 6138 0 6163 116 0 18 BC 6117 0 6141 98 0 19 BD 2539 0 2520 69 0 Continued on next page... Page 65 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Non-H H(model) H(added) Clashes Symm-Clashes 20 BE 5936 0 5882 137 0 21 CA 1582 0 1528 35 0 22 CB 8870 0 8975 149 0 23 E1 1689 0 1738 34 0 23 E2 1695 0 1757 38 0 24 E3 2114 0 2154 55 0 25 E4 1425 0 322 18 0 26 K1 1410 0 1503 27 0 27 MA 1097 0 1130 7 0 28 MB 1465 0 1486 31 0 29 MC 307 0 231 2 0 30 P1 1368 0 1436 9 0 31 R1 2742 0 2829 40 0 32 R2 2228 0 2228 15 0 33 S1 1425 0 334 35 0 34 SA 23759 0 11991 338 0 35 SC 1709 0 1784 47 0 36 SF 1881 0 1958 50 0 37 SG 1609 0 1675 41 0 38 SH 1369 0 1414 69 0 39 SI 1322 0 1394 29 0 40 SJ 1350 0 1374 41 0 41 SK 1412 0 1486 22 0 42 SM 1143 0 1210 26 0 43 SN 890 0 887 12 0 44 SO 1087 0 1152 16 0 45 SP 750 0 728 9 0 46 SR 973 0 1029 37 0 47 SX 1021 0 1060 27 0 48 SY 785 0 840 20 0 49 SZ 801 0 828 13 0 50 Sc 595 0 613 0 0 51 Sd 497 0 535 0 0 52 Sf 251 0 277 0 0 53 Sg 397 0 399 0 0 54 U1 1425 0 329 23 0 55 U2 365 0 75 0 0 56 U3 7035 0 1590 5 0 57 U4 990 0 1054 13 0 58 U5 2009 0 2130 16 0 59 UA 1690 0 370 0 0 60 UB 2775 0 620 1 0 Continued on next page... Page 66 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Non-H H(model) H(added) Clashes Symm-Clashes 61 UC 3300 0 727 12 0 All All 173863 0 129519 2467 0

The all-atom clashscore is defined as the number of clashes found per 1000 atoms (including hydrogen atoms). The all-atom clashscore for this structure is 9. All (2467) close contacts within the same asymmetric unit are listed below, sorted by their clash magnitude.

Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 12:AE:630:TYR:CE1 12:AE:637:ILE:CD1 1.86 1.56 34:SA:283:U:N3 34:SA:283:U:C2 1.75 1.54 34:SA:283:U:N1 34:SA:283:U:C6 1.70 1.54 34:SA:283:U:N3 34:SA:283:U:C4 1.74 1.53 34:SA:283:U:C2 34:SA:283:U:N1 1.74 1.52 34:SA:283:U:N1 38:SH:188:ARG:CG 1.79 1.44 34:SA:283:U:N1 38:SH:188:ARG:HG3 1.04 1.37 12:AE:630:TYR:CZ 12:AE:637:ILE:HD13 1.63 1.33 12:AE:630:TYR:CE1 12:AE:637:ILE:HD12 1.52 1.32 38:SH:188:ARG:NH1 38:SH:191:ARG:CD 2.05 1.20 12:AE:571:LEU:C 12:AE:571:LEU:CA 2.11 1.19 38:SH:188:ARG:NH1 38:SH:191:ARG:HD2 1.57 1.19 24:E3:206:LEU:H 24:E3:207:PRO:CD 1.54 1.17 34:SA:283:U:H5” 38:SH:188:ARG:HD2 1.23 1.17 12:AE:630:TYR:OH 12:AE:637:ILE:HD13 1.43 1.17 12:AE:630:TYR:CE1 12:AE:637:ILE:HD13 1.66 1.16 34:SA:283:U:OP2 38:SH:188:ARG:CZ 1.95 1.12 12:AE:571:LEU:HB3 12:AE:630:TYR:CD2 1.83 1.12 38:SH:188:ARG:NH2 38:SH:191:ARG:HH21 1.53 1.07 12:AE:630:TYR:HE1 12:AE:637:ILE:CB 1.68 1.07 12:AE:630:TYR:HE1 12:AE:637:ILE:CG1 1.67 1.06 24:E3:206:LEU:H 24:E3:207:PRO:HD3 1.08 1.05 16:BA:538:GLN:HA 16:BA:552:ASN:O 1.57 1.03 34:SA:283:U:C1’ 38:SH:188:ARG:HG3 1.88 1.03 12:AE:630:TYR:HE1 12:AE:637:ILE:CD1 1.37 1.02 34:SA:126:A:H62 34:SA:291:G:N2 1.57 1.02 34:SA:126:A:N6 34:SA:291:G:H21 1.57 1.02 1:3A:254:A:OP2 6:3G:95:ARG:NH1 1.91 1.02 12:AE:630:TYR:CZ 12:AE:637:ILE:CD1 2.31 1.01 38:SH:188:ARG:CZ 38:SH:191:ARG:HD2 1.94 0.98 34:SA:283:U:C5’ 38:SH:188:ARG:HD2 1.93 0.98 34:SA:877:G:H1 34:SA:951:A:H61 1.03 0.98 Continued on next page... Page 67 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 15:B1:283:LEU:O 15:B1:788:TYR:HB3 1.64 0.97 12:AE:630:TYR:CE1 12:AE:637:ILE:CB 2.47 0.97 34:SA:1175:U:H3 34:SA:1464:G:H1 1.03 0.97 12:AE:630:TYR:CE1 12:AE:637:ILE:HG21 2.00 0.97 34:SA:330:G:OP2 40:SJ:172:ARG:NH1 1.99 0.96 38:SH:188:ARG:HH12 38:SH:191:ARG:HD3 1.32 0.95 8:AA:66:UNK:O 8:AA:77:UNK:HA 1.67 0.95 15:B1:847:LEU:O 15:B1:853:ARG:HA 1.67 0.94 34:SA:1213:G:H1 34:SA:1450:U:H3 1.10 0.94 1:3A:7:G:H1 34:SA:1117:U:H3 1.08 0.94 5:3F:421:ASN:HA 5:3F:436:GLU:O 1.68 0.93 20:BE:27:PHE:HB3 20:BE:653:ILE:O 1.68 0.93 7:5A:506:G:H1 7:5A:530:A:H61 1.04 0.92 5:3F:539:ILE:O 5:3F:565:SER:HA 1.70 0.92 17:BB:597:ILE:O 17:BB:611:LEU:HB2 1.69 0.92 7:5A:506:G:H1 7:5A:530:A:N6 1.67 0.92 34:SA:1663:G:H1 34:SA:1738:U:H3 0.92 0.91 34:SA:283:U:H5” 38:SH:188:ARG:CD 2.00 0.91 28:MB:197:ALA:O 28:MB:200:LYS:NZ 2.04 0.91 20:BE:631:ASN:O 20:BE:643:THR:HA 1.71 0.90 24:E3:206:LEU:N 24:E3:207:PRO:CD 2.27 0.90 7:5A:250:G:H1 7:5A:262:U:H3 0.95 0.90 34:SA:283:U:C4 38:SH:188:ARG:HB2 2.06 0.90 47:SX:23:ARG:HH12 47:SX:66:ASN:HA 1.35 0.89 19:BD:367:GLN:O 19:BD:386:ILE:HA 1.70 0.89 5:3F:497:LYS:NZ 5:3F:513:GLU:OE2 2.05 0.89 38:SH:188:ARG:NH1 38:SH:191:ARG:HD3 1.88 0.89 8:AA:359:UNK:HA 8:AA:373:UNK:O 1.72 0.89 6:3G:57:ASP:O 6:3G:84:ARG:NH1 2.06 0.89 34:SA:1219:A:H62 34:SA:1264:G:N2 1.71 0.88 12:AE:571:LEU:CB 12:AE:630:TYR:CD2 2.56 0.88 17:BB:555:VAL:O 17:BB:568:SER:HA 1.72 0.88 12:AE:630:TYR:CE1 12:AE:637:ILE:HB 2.08 0.88 15:B1:281:GLU:HB2 15:B1:790:ARG:O 1.73 0.87 12:AE:571:LEU:CA 12:AE:630:TYR:CD2 2.57 0.87 37:SG:84:LYS:HG2 37:SG:92:ARG:HH12 1.40 0.87 1:3A:326:U:OP1 6:3H:46:ARG:NH1 2.09 0.86 12:AE:630:TYR:CE1 12:AE:637:ILE:CG2 2.58 0.86 5:3F:235:HIS:ND1 5:3F:257:ASP:OD2 2.09 0.86 5:3F:347:MET:HA 5:3F:357:LEU:O 1.75 0.86 34:SA:877:G:H1 34:SA:951:A:N6 1.74 0.86 Continued on next page... Page 68 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 19:BD:482:LEU:HB2 19:BD:494:TYR:O 1.76 0.85 12:AE:571:LEU:C 12:AE:630:TYR:CD2 2.49 0.85 36:SF:153:ASN:O 36:SF:174:LYS:NZ 2.10 0.84 22:CB:150:ASP:O 22:CB:154:LYS:HB2 1.77 0.84 13:AF:75:UNK:HA 13:AF:89:UNK:O 1.77 0.84 34:SA:1529:C:OP1 37:SG:112:ARG:NH1 2.10 0.84 34:SA:523:G:N2 34:SA:529:A:OP2 2.10 0.84 39:SI:76:LYS:HG2 39:SI:79:ARG:HH12 1.42 0.84 5:3F:146:LYS:O 5:3F:566:ALA:HA 1.77 0.84 7:5A:513:G:H1 7:5A:523:U:H3 1.25 0.84 31:R1:29:LYS:NZ 31:R1:332:ILE:O 2.10 0.84 40:SJ:110:ARG:NH1 40:SJ:160:PHE:O 2.10 0.84 1:3A:84:U:OP2 4:3E:361:ARG:NH2 2.11 0.83 26:K1:124:ASP:OD1 26:K1:184:ARG:NH1 2.11 0.83 36:SF:75:LYS:NZ 36:SF:77:ARG:HH12 1.76 0.83 34:SA:1482:C:N4 34:SA:1525:A:OP2 2.10 0.83 22:CB:536:TYR:OH 22:CB:552:ARG:NH1 2.12 0.83 8:AA:360:UNK:O 8:AA:372:UNK:HA 1.77 0.82 34:SA:283:U:N3 38:SH:188:ARG:HB2 1.92 0.82 42:SM:72:THR:O 42:SM:88:ARG:NH1 2.12 0.82 5:3F:551:ARG:NH1 6:3G:93:VAL:O 2.12 0.82 17:BB:554:THR:HA 17:BB:569:LEU:O 1.80 0.82 34:SA:1588:G:H1 34:SA:1608:U:H3 0.85 0.82 22:CB:152:PHE:O 22:CB:156:LYS:NZ 2.13 0.82 22:CB:307:LYS:NZ 34:SA:1683:C:OP1 2.13 0.81 20:BE:423:ARG:NH1 20:BE:426:GLU:OE1 2.13 0.81 12:AE:571:LEU:C 12:AE:571:LEU:HA 1.98 0.81 34:SA:322:G:O2’ 40:SJ:10:LYS:NZ 2.13 0.81 1:3A:5:A:H2 34:SA:1119:G:H1 1.22 0.81 34:SA:396:G:N1 34:SA:399:A:OP2 2.13 0.81 1:3A:5:A:C2 34:SA:1119:G:N1 2.48 0.81 5:3F:495:SER:HA 5:3F:514:LEU:O 1.80 0.81 22:CB:779:PHE:HA 22:CB:849:GLY:O 1.80 0.81 20:BE:24:PHE:HA 20:BE:655:THR:O 1.81 0.80 47:SX:20:THR:OG1 47:SX:22:LYS:NZ 2.13 0.80 20:BE:66:LEU:O 20:BE:346:ARG:NH1 2.12 0.80 22:CB:707:PHE:O 22:CB:918:ARG:NH1 2.14 0.80 34:SA:283:U:OP2 38:SH:188:ARG:NH2 2.14 0.80 34:SA:1216:C:O2’ 34:SA:1217:A:O5’ 1.97 0.80 14:AG:118:UNK:O 14:AG:130:UNK:HA 1.81 0.80 22:CB:367:ARG:O 22:CB:371:GLN:HB2 1.82 0.80 Continued on next page... Page 69 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 42:SM:132:SER:O 42:SM:136:ARG:NH1 2.14 0.80 15:B1:988:ARG:HH12 28:MB:146:ARG:HH11 1.29 0.80 34:SA:1636:C:H42 34:SA:1765:A:H61 1.28 0.79 12:AE:571:LEU:CB 12:AE:630:TYR:CE2 2.66 0.79 15:B1:845:LEU:O 15:B1:855:GLN:HA 1.81 0.79 20:BE:796:LEU:O 20:BE:800:GLU:HB2 1.83 0.79 23:E1:178:VAL:HA 23:E1:223:GLU:O 1.83 0.79 34:SA:163:G:OP2 34:SA:163:G:N2 2.14 0.79 3:3D:158:TYR:O 3:3D:162:LYS:HB2 1.82 0.79 24:E3:334:GLU:O 24:E3:338:ALA:HB3 1.83 0.78 34:SA:1085:G:N2 34:SA:1088:A:OP2 2.16 0.78 20:BE:356:ILE:O 20:BE:633:LYS:NZ 2.16 0.78 34:SA:1584:G:N2 34:SA:1611:A:OP2 2.17 0.78 36:SF:75:LYS:HZ2 36:SF:77:ARG:NH1 1.80 0.78 19:BD:382:GLY:N 19:BD:405:THR:HG1 1.81 0.78 23:E2:44:VAL:HA 23:E2:113:TYR:O 1.83 0.78 34:SA:522:U:OP1 49:SZ:37:LYS:NZ 2.16 0.78 8:AA:524:UNK:O 8:AA:536:UNK:HA 1.84 0.77 1:3A:80:U:OP2 6:3H:95:ARG:NH1 2.17 0.77 23:E2:88:ARG:NH1 34:SA:1191:U:OP1 2.17 0.77 26:K1:141:ARG:NH1 26:K1:191:MET:O 2.16 0.77 38:SH:188:ARG:NH2 38:SH:191:ARG:NH2 2.32 0.77 36:SF:50:ASN:O 36:SF:53:LYS:NZ 2.18 0.77 6:3H:57:ASP:O 6:3H:84:ARG:NH1 2.18 0.77 12:AE:571:LEU:HB3 12:AE:630:TYR:CE2 2.19 0.77 36:SF:11:ARG:NH1 36:SF:21:ASP:OD1 2.18 0.76 38:SH:188:ARG:HH22 38:SH:191:ARG:HH21 1.34 0.76 34:SA:1253:U:OP1 43:SN:50:LYS:NZ 2.18 0.76 14:AG:382:UNK:HA 14:AG:397:UNK:O 1.86 0.76 23:E1:42:ILE:O 23:E1:203:CYS:HA 1.86 0.76 35:SC:158:SER:O 35:SC:162:ARG:NH1 2.19 0.76 1:3A:206:C:N4 1:3A:242:U:OP2 2.18 0.76 20:BE:439:PHE:HA 20:BE:455:PHE:O 1.86 0.76 22:CB:921:ASP:OD2 22:CB:1172:ASN:ND2 2.19 0.76 12:AE:571:LEU:C 12:AE:630:TYR:HD2 1.89 0.75 34:SA:364:G:OP2 34:SA:377:G:N2 2.18 0.75 34:SA:1216:C:O2’ 34:SA:1217:A:P 2.44 0.75 24:E3:206:LEU:N 24:E3:207:PRO:HD3 1.85 0.75 34:SA:335:U:O2’ 42:SM:129:ARG:NH1 2.19 0.75 9:AB:190:UNK:O 9:AB:201:UNK:HA 1.87 0.75 6:3H:29:ASN:O 6:3H:31:ARG:NH1 2.20 0.74 Continued on next page... Page 70 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 24:E3:301:TYR:O 24:E3:304:TYR:N 2.20 0.74 16:BA:21:THR:O 16:BA:30:LEU:HB2 1.87 0.74 26:K1:164:ILE:HA 26:K1:172:SER:O 1.88 0.74 2:3B:170:VAL:HA 2:3B:239:CYS:O 1.88 0.74 9:AB:110:UNK:O 9:AB:117:UNK:HA 1.87 0.73 1:3A:67:G:H1 7:5A:286:U:H3 1.37 0.73 14:AG:4:UNK:HA 14:AG:569:UNK:O 1.87 0.73 15:B1:607:ASP:O 31:R1:321:ARG:NH1 2.19 0.73 12:AE:571:LEU:CA 12:AE:630:TYR:CE2 2.71 0.72 34:SA:432:G:OP2 34:SA:433:C:N4 2.22 0.72 15:B1:610:LYS:NZ 15:B1:612:VAL:O 2.21 0.72 26:K1:198:TYR:O 26:K1:202:GLU:HB2 1.90 0.72 20:BE:132:THR:OG1 20:BE:134:ASP:OD1 2.06 0.72 12:AE:756:LEU:O 12:AE:760:PHE:HB2 1.89 0.72 22:CB:100:SER:O 22:CB:104:LYS:HB2 1.88 0.72 22:CB:1097:ASP:OD2 22:CB:1186:ILE:N 2.16 0.72 22:CB:899:LEU:O 22:CB:903:SER:HB3 1.89 0.72 23:E2:41:MET:O 23:E2:110:LEU:HA 1.89 0.72 1:3A:83:A:OP2 4:3E:361:ARG:NE 2.23 0.72 36:SF:75:LYS:HZ2 36:SF:77:ARG:HH12 1.33 0.72 24:E3:301:TYR:CE2 34:SA:1217:A:N6 2.57 0.72 38:SH:188:ARG:HH12 38:SH:191:ARG:CD 1.88 0.72 34:SA:283:U:C2 34:SA:283:U:C1’ 2.73 0.72 34:SA:405:C:O4’ 38:SH:88:ARG:NH1 2.22 0.71 46:SR:42:GLU:HG3 46:SR:45:ARG:HH12 1.55 0.71 36:SF:118:GLU:OE2 36:SF:237:SER:N 2.23 0.71 41:SK:163:PRO:HA 41:SK:166:GLY:H 1.55 0.71 12:AE:120:TRP:O 12:AE:124:ARG:HB3 1.91 0.71 9:AB:271:UNK:HA 9:AB:284:UNK:O 1.90 0.71 17:BB:186:ILE:HB 17:BB:200:HIS:O 1.90 0.71 7:5A:337:G:H21 7:5A:338:A:H62 1.39 0.71 28:MB:234:ARG:NH1 28:MB:254:PHE:O 2.22 0.71 34:SA:538:A:H1’ 34:SA:540:G:H1 1.56 0.71 16:BA:60:ALA:O 16:BA:72:SER:HA 1.91 0.70 19:BD:577:LEU:O 19:BD:588:LEU:HA 1.91 0.70 24:E3:206:LEU:H 24:E3:207:PRO:HD2 1.52 0.70 34:SA:337:G:OP2 34:SA:338:C:N4 2.24 0.70 12:AE:746:GLU:O 12:AE:750:ASN:HB2 1.91 0.70 16:BA:198:PHE:O 16:BA:207:TYR:HB3 1.91 0.70 34:SA:1174:C:H42 34:SA:1465:C:H42 1.39 0.70 15:B1:943:LYS:NZ 34:SA:572:C:OP2 2.14 0.70 Continued on next page... Page 71 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 20:BE:273:LEU:O 20:BE:286:VAL:HA 1.91 0.70 37:SG:76:ARG:NH1 46:SR:120:ASP:OD1 2.23 0.70 2:3C:299:LYS:HD2 2:3C:322:ARG:HB3 1.73 0.70 17:BB:447:LEU:O 17:BB:454:LEU:HA 1.90 0.70 20:BE:84:SER:HB2 20:BE:91:TYR:HB2 1.72 0.69 5:3F:198:LYS:NZ 5:3F:265:THR:O 2.25 0.69 19:BD:313:PHE:HA 19:BD:322:HIS:O 1.92 0.69 1:3A:1:G:C6 34:SA:1124:A:N1 2.60 0.69 18:BC:662:GLN:O 18:BC:666:GLU:HB2 1.93 0.69 18:BC:722:GLU:O 18:BC:725:ASP:HB2 1.92 0.69 19:BD:369:ASN:O 19:BD:384:ILE:HA 1.91 0.69 18:BC:698:LEU:O 18:BC:702:LEU:HB2 1.92 0.69 15:B1:200:LEU:O 15:B1:204:ARG:HB2 1.92 0.69 5:3F:155:ASN:N 5:3F:193:ASP:OD2 2.22 0.69 21:CA:134:ILE:O 21:CA:138:TRP:HB2 1.93 0.69 48:SY:67:ALA:HB3 48:SY:69:ARG:HH12 1.58 0.69 19:BD:311:ASN:HA 19:BD:324:TRP:O 1.92 0.69 18:BC:190:THR:OG1 22:CB:657:ARG:NH1 2.25 0.68 34:SA:1217:A:H2 34:SA:1448:G:H21 1.41 0.68 34:SA:938:G:N2 34:SA:941:A:OP2 2.19 0.68 5:3F:299:CYS:HB2 5:3F:303:LYS:O 1.92 0.68 21:CA:18:PHE:O 21:CA:34:LEU:HA 1.94 0.68 34:SA:867:G:H1 34:SA:961:U:H3 1.38 0.68 38:SH:187:LYS:HZ1 38:SH:191:ARG:HH12 1.39 0.68 19:BD:325:ASP:HB2 19:BD:337:ALA:H 1.57 0.68 12:AE:571:LEU:CA 12:AE:630:TYR:HD2 2.06 0.68 36:SF:59:ARG:HA 36:SF:62:LYS:HZ3 1.59 0.68 12:AE:630:TYR:CD1 12:AE:637:ILE:HB 2.28 0.68 34:SA:1219:A:N6 34:SA:1264:G:N2 2.40 0.68 7:5A:511:G:H1 7:5A:525:U:H3 1.42 0.67 16:BA:103:LYS:O 16:BA:112:ALA:HB3 1.94 0.67 19:BD:424:ILE:HB 19:BD:433:TRP:HB3 1.75 0.67 12:AE:367:LEU:O 12:AE:371:LYS:HB2 1.95 0.67 14:AG:423:UNK:HA 14:AG:438:UNK:O 1.94 0.67 36:SF:11:ARG:NH1 36:SF:21:ASP:O 2.26 0.67 40:SJ:105:ASP:OD2 40:SJ:107:THR:OG1 2.11 0.67 42:SM:36:LYS:NZ 42:SM:59:PRO:O 2.24 0.67 17:BB:488:LEU:HB2 17:BB:500:TRP:HB2 1.77 0.67 17:BB:851:PRO:O 17:BB:855:LYS:HB2 1.94 0.67 14:AG:402:UNK:HA 14:AG:416:UNK:O 1.95 0.67 34:SA:283:U:C1’ 34:SA:283:U:C6 2.71 0.67 Continued on next page... Page 72 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 17:BB:393:ASP:HA 17:BB:679:HIS:HB3 1.77 0.67 20:BE:309:ILE:HG12 20:BE:323:VAL:HG12 1.75 0.67 12:AE:597:HIS:HB3 12:AE:615:ASN:HB3 1.77 0.66 5:3F:189:THR:O 5:3F:196:LEU:HA 1.94 0.66 34:SA:323:A:H61 34:SA:345:U:H3 1.43 0.66 34:SA:394:C:O2 34:SA:401:A:N6 2.29 0.66 2:3B:298:ILE:HA 2:3B:319:ARG:O 1.96 0.66 22:CB:182:ASP:HB2 22:CB:208:THR:HB 1.78 0.66 34:SA:283:U:C5 38:SH:188:ARG:NE 2.50 0.66 20:BE:477:ILE:O 20:BE:484:ILE:HA 1.96 0.66 38:SH:188:ARG:HH11 38:SH:191:ARG:HB2 1.61 0.66 7:5A:348:U:H3 7:5A:376:U:H3 1.42 0.66 16:BA:59:ALA:HA 16:BA:73:ILE:O 1.96 0.66 35:SC:144:ARG:HB3 35:SC:208:GLN:HB3 1.76 0.66 19:BD:411:TYR:OH 19:BD:423:LEU:N 2.29 0.66 20:BE:567:ILE:O 20:BE:580:GLN:HA 1.96 0.66 23:E2:44:VAL:HG22 23:E2:113:TYR:HB2 1.76 0.66 26:K1:140:LYS:O 26:K1:144:ARG:HB2 1.96 0.66 38:SH:79:LYS:HG3 38:SH:86:PRO:HG2 1.77 0.66 7:5A:538:C:H2’ 7:5A:539:A:H8 1.60 0.66 15:B1:827:ALA:HA 15:B1:920:GLU:H 1.61 0.66 20:BE:97:LYS:HZ2 20:BE:111:GLU:HB2 1.60 0.66 34:SA:629:U:OP2 34:SA:969:C:N4 2.28 0.66 39:SI:143:LEU:HD23 39:SI:145:GLY:H 1.60 0.66 7:5A:557:A:H61 7:5A:580:A:H61 1.43 0.66 5:3F:322:HIS:HB2 5:3F:343:ASP:HB3 1.78 0.65 12:AE:559:ASN:HB2 12:AE:616:PRO:HD3 1.77 0.65 34:SA:283:U:N1 38:SH:188:ARG:CB 2.58 0.65 22:CB:412:LEU:HD21 22:CB:424:GLY:HA3 1.78 0.65 34:SA:1163:A:H4’ 37:SG:166:ARG:HH12 1.60 0.65 5:3F:335:ARG:HA 5:3F:349:TRP:O 1.96 0.65 20:BE:481:ASN:O 20:BE:499:LYS:NZ 2.30 0.65 28:MB:152:LEU:HB3 28:MB:165:PHE:HB2 1.78 0.65 39:SI:51:VAL:HG13 39:SI:53:GLY:H 1.61 0.65 18:BC:697:VAL:HG12 18:BC:700:ARG:HH21 1.61 0.65 28:MB:94:ARG:NH1 34:SA:1588:G:N7 2.45 0.65 7:5A:334:G:H1’ 27:MA:55:ARG:HE 1.62 0.65 20:BE:630:THR:N 20:BE:644:THR:O 2.30 0.65 7:5A:503:C:H1’ 7:5A:535:G:H1 1.61 0.65 8:AA:68:UNK:O 8:AA:75:UNK:HA 1.96 0.65 3:3D:23:GLN:HG2 3:3D:50:LEU:HA 1.79 0.65 Continued on next page... Page 73 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 36:SF:180:LEU:HA 36:SF:194:THR:HG22 1.77 0.65 2:3B:194:VAL:HB 2:3B:217:ILE:HA 1.78 0.65 15:B1:988:ARG:HH12 28:MB:146:ARG:NH1 1.94 0.65 17:BB:838:THR:O 17:BB:842:ASN:HB2 1.97 0.65 34:SA:1219:A:N6 34:SA:1264:G:H21 1.95 0.65 12:AE:579:ARG:HD2 12:AE:631:VAL:HG22 1.80 0.64 23:E2:39:LYS:O 23:E2:109:LYS:NZ 2.22 0.64 34:SA:537:G:OP2 41:SK:175:ARG:NH2 2.27 0.64 5:3F:496:LEU:HB2 5:3F:514:LEU:HB2 1.79 0.64 20:BE:223:LEU:HB2 20:BE:231:ILE:HB 1.79 0.64 24:E3:305:ARG:O 24:E3:307:VAL:N 2.30 0.64 8:AA:218:UNK:HA 8:AA:234:UNK:HA 1.79 0.64 20:BE:609:ILE:HB 20:BE:623:ILE:HB 1.78 0.64 19:BD:483:ALA:H 19:BD:523:PHE:HZ 1.44 0.64 12:AE:37:SER:O 12:AE:41:ALA:HB2 1.98 0.64 23:E2:164:LYS:HZ1 34:SA:1575:G:P 2.19 0.64 31:R1:18:ARG:NH1 31:R1:98:TYR:O 2.29 0.64 37:SG:53:VAL:HG22 37:SG:55:ASP:H 1.62 0.64 5:3F:551:ARG:HH11 6:3G:94:SER:HA 1.63 0.64 8:AA:486:UNK:HA 8:AA:492:UNK:HA 1.78 0.64 16:BA:712:PHE:H 16:BA:746:ASN:HD21 1.43 0.64 20:BE:467:ALA:HB3 20:BE:476:PHE:HB2 1.80 0.64 24:E3:301:TYR:HA 24:E3:304:TYR:HB2 1.80 0.64 14:AG:485:UNK:O 14:AG:497:UNK:HA 1.98 0.63 16:BA:715:PHE:HB2 16:BA:744:ARG:HG2 1.80 0.63 20:BE:179:LYS:HA 20:BE:191:PHE:O 1.98 0.63 20:BE:430:ILE:HB 20:BE:443:TRP:HB2 1.79 0.63 20:BE:350:SER:N 20:BE:372:ASP:OD2 2.30 0.63 22:CB:99:LYS:O 22:CB:103:PHE:HB3 1.98 0.63 24:E3:301:TYR:CE2 34:SA:1217:A:C6 2.86 0.63 37:SG:68:ILE:HG22 46:SR:114:ARG:HH12 1.64 0.63 12:AE:465:PHE:HB2 12:AE:507:LYS:HG2 1.81 0.63 34:SA:283:U:C2 38:SH:188:ARG:CB 2.82 0.63 3:3D:122:GLU:HG2 3:3D:124:ALA:H 1.63 0.63 22:CB:840:LEU:HB3 22:CB:852:PHE:HB2 1.79 0.63 35:SC:130:SER:HG 35:SC:180:THR:HG1 1.44 0.63 38:SH:31:ARG:HG2 38:SH:101:ILE:HG12 1.80 0.63 42:SM:71:LEU:HB3 42:SM:88:ARG:NH1 2.13 0.63 7:5A:174:U:H3 7:5A:222:G:H1 1.47 0.63 15:B1:143:MET:N 48:SY:144:ARG:HH11 1.97 0.63 21:CA:192:GLU:O 21:CA:196:ALA:HB2 1.98 0.63 Continued on next page... Page 74 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 12:AE:465:PHE:H 12:AE:507:LYS:HE2 1.63 0.63 1:3A:5:A:N1 34:SA:1119:G:O6 2.31 0.63 20:BE:551:TYR:HD1 20:BE:558:PHE:HB3 1.64 0.63 22:CB:861:ILE:O 22:CB:865:ARG:HB2 1.99 0.63 24:E3:418:HIS:O 24:E3:422:LEU:HB2 1.99 0.63 7:5A:506:G:N2 7:5A:530:A:N1 2.47 0.62 16:BA:842:ASN:ND2 20:BE:882:GLU:OE1 2.32 0.62 18:BC:771:LYS:HB3 18:BC:775:ILE:HD12 1.80 0.62 21:CA:55:LEU:O 21:CA:123:THR:HA 1.98 0.62 34:SA:929:A:H4’ 34:SA:930:A:OP2 1.98 0.62 5:3F:138:ASP:OD1 5:3F:500:LYS:NZ 2.31 0.62 16:BA:817:PHE:O 16:BA:821:MET:HB2 1.99 0.62 20:BE:266:VAL:HB 20:BE:274:ILE:HB 1.81 0.62 15:B1:159:GLN:NE2 34:SA:518:A:OP2 2.32 0.62 24:E3:301:TYR:CZ 34:SA:1217:A:C6 2.88 0.62 1:3A:12:U:H3 34:SA:1112:G:H1 1.46 0.62 1:3A:5:A:H2 34:SA:1119:G:N1 1.92 0.62 20:BE:596:GLU:HG3 20:BE:598:ARG:NH1 2.14 0.62 22:CB:525:LEU:HB2 22:CB:617:LEU:HB2 1.80 0.62 22:CB:530:GLN:HB2 22:CB:695:GLN:HB2 1.81 0.62 34:SA:51:A:H62 34:SA:429:G:H21 1.47 0.62 40:SJ:172:ARG:HE 40:SJ:175:GLN:HG3 1.65 0.62 8:AA:523:UNK:HA 8:AA:537:UNK:O 2.00 0.62 12:AE:365:ILE:O 12:AE:369:LEU:HB3 1.99 0.62 18:BC:318:GLU:HB3 18:BC:327:ILE:HG22 1.82 0.62 40:SJ:5:ARG:NH2 40:SJ:27:PHE:O 2.33 0.62 12:AE:571:LEU:C 12:AE:630:TYR:CE2 2.73 0.62 20:BE:861:ILE:HD11 20:BE:905:ILE:HD12 1.82 0.62 21:CA:16:VAL:HB 21:CA:37:MET:O 1.99 0.62 40:SJ:103:GLN:HB3 40:SJ:164:ARG:NH1 2.15 0.62 34:SA:411:C:H1’ 34:SA:423:G:H22 1.65 0.61 46:SR:73:GLY:H 46:SR:76:SER:HB3 1.63 0.61 48:SY:107:PHE:HB3 48:SY:114:LYS:HD3 1.81 0.61 17:BB:426:ARG:HG2 17:BB:462:SER:HA 1.82 0.61 22:CB:1070:VAL:O 22:CB:1074:LEU:HB2 2.00 0.61 35:SC:199:ASN:HA 35:SC:202:LYS:HE3 1.81 0.61 36:SF:88:ASP:HB2 36:SF:101:LEU:HD12 1.82 0.61 42:SM:7:VAL:HG11 42:SM:53:TYR:HA 1.82 0.61 47:SX:118:ARG:NH1 47:SX:119:LYS:NZ 2.47 0.61 34:SA:1219:A:N6 34:SA:1264:G:C2 2.68 0.61 34:SA:273:G:N7 38:SH:185:GLN:NE2 2.49 0.61 Continued on next page... Page 75 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 1:3A:53:U:H3 7:5A:465:G:H1 1.49 0.61 18:BC:108:LEU:HD13 18:BC:150:LEU:HD22 1.82 0.61 20:BE:900:ASN:OD1 20:BE:903:GLN:NE2 2.34 0.61 32:R2:1416:GLN:HE21 32:R2:1559:ILE:HD12 1.66 0.61 5:3F:234:GLY:O 5:3F:259:LYS:NZ 2.31 0.61 26:K1:92:ALA:HB2 45:SP:102:LEU:HD12 1.81 0.61 36:SF:17:HIS:HA 36:SF:108:ARG:HG2 1.81 0.61 40:SJ:70:GLU:OE1 42:SM:24:LYS:NZ 2.33 0.61 4:3E:76:LEU:HD23 4:3E:84:LYS:NZ 2.15 0.61 30:P1:188:LYS:HA 30:P1:191:LYS:HZ3 1.65 0.61 38:SH:79:LYS:HE2 38:SH:90:GLY:H 1.64 0.61 2:3C:162:LEU:HG 2:3C:164:ILE:H 1.65 0.61 15:B1:112:LYS:HG3 15:B1:113:ILE:HG23 1.82 0.61 17:BB:251:GLN:HE21 17:BB:344:THR:HG23 1.66 0.61 17:BB:888:ARG:O 17:BB:892:LYS:HB2 2.00 0.61 24:E3:301:TYR:O 24:E3:304:TYR:CB 2.49 0.61 31:R1:143:LYS:HZ1 31:R1:252:LYS:HB3 1.66 0.61 1:3A:5:A:N1 34:SA:1119:G:C6 2.68 0.61 15:B1:988:ARG:HH22 28:MB:146:ARG:HE 1.49 0.61 17:BB:218:ILE:HA 17:BB:227:LYS:O 2.01 0.61 20:BE:229:GLU:HA 20:BE:244:ILE:O 2.00 0.61 22:CB:679:SER:O 22:CB:683:ASN:HB2 2.01 0.61 23:E1:46:ALA:HA 23:E1:115:GLN:HB3 1.82 0.61 34:SA:102:U:O2’ 34:SA:105:A:N6 2.34 0.61 42:SM:21:ASN:ND2 42:SM:30:ARG:O 2.34 0.61 5:3F:311:GLN:HB3 49:SZ:4:ALA:HB1 1.83 0.60 16:BA:82:VAL:HG12 16:BA:88:ASN:H 1.65 0.60 19:BD:462:GLY:HA2 19:BD:480:ARG:H 1.64 0.60 20:BE:569:VAL:HB 20:BE:579:ARG:HB2 1.84 0.60 26:K1:49:ARG:NH2 26:K1:108:VAL:O 2.34 0.60 40:SJ:74:LYS:HD3 40:SJ:108:PRO:HB2 1.83 0.60 5:3F:424:LEU:HB3 5:3F:434:PHE:HB3 1.82 0.60 20:BE:308:PRO:HB2 20:BE:324:PHE:HB2 1.82 0.60 23:E1:94:GLN:OE1 23:E2:132:ARG:NH1 2.34 0.60 3:3D:34:VAL:HG23 3:3D:36:GLU:H 1.67 0.60 34:SA:51:A:H62 34:SA:429:G:N2 1.98 0.60 38:SH:70:PRO:HB3 38:SH:101:ILE:HB 1.83 0.60 5:3F:497:LYS:HG2 5:3F:513:GLU:HG2 1.84 0.60 13:AF:28:UNK:O 13:AF:35:UNK:HA 2.01 0.60 18:BC:45:LEU:HD12 18:BC:54:HIS:HB3 1.82 0.60 21:CA:204:SER:O 21:CA:208:ASP:HB2 2.00 0.60 Continued on next page... Page 76 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:900:A:H3’ 34:SA:901:G:H21 1.65 0.60 16:BA:541:ILE:HG22 16:BA:550:VAL:HG22 1.84 0.60 47:SX:31:SER:H 47:SX:34:ILE:HD12 1.67 0.60 16:BA:533:SER:HB3 16:BA:580:PHE:HB2 1.83 0.60 17:BB:799:LYS:HD2 17:BB:800:THR:HG23 1.83 0.60 18:BC:83:GLN:NE2 18:BC:103:SER:O 2.35 0.60 20:BE:230:VAL:O 20:BE:243:THR:HA 2.02 0.60 28:MB:162:THR:HB 28:MB:262:ARG:HB3 1.83 0.60 34:SA:1216:C:HO2’ 34:SA:1217:A:P 2.23 0.60 34:SA:364:G:H3’ 34:SA:365:G:H8 1.67 0.60 17:BB:135:ARG:NH1 17:BB:149:ASP:OD1 2.35 0.60 20:BE:587:ARG:HB3 20:BE:605:LEU:HD13 1.83 0.60 38:SH:188:ARG:HH11 38:SH:191:ARG:CB 2.15 0.60 1:3A:64:A:H4’ 20:BE:392:ARG:HH12 1.66 0.60 5:3F:304:ILE:HB 5:3F:318:LEU:HB2 1.83 0.60 8:AA:282:UNK:O 8:AA:290:UNK:N 2.35 0.60 13:AF:67:UNK:O 13:AF:78:UNK:HA 2.01 0.60 16:BA:169:SER:HB2 16:BA:186:THR:HB 1.84 0.60 17:BB:397:ILE:HG22 17:BB:408:THR:HG22 1.84 0.60 22:CB:812:GLU:O 22:CB:816:ALA:HB2 2.01 0.60 5:3F:187:ALA:HB3 5:3F:199:TYR:HB2 1.84 0.60 22:CB:268:LEU:HD23 22:CB:294:LEU:HD12 1.84 0.60 34:SA:477:A:N6 34:SA:540:G:O6 2.34 0.60 17:BB:828:TYR:O 17:BB:832:PHE:HB2 2.02 0.59 20:BE:641:LEU:O 20:BE:653:ILE:HA 2.02 0.59 15:B1:609:GLU:OE2 31:R1:17:PHE:N 2.35 0.59 43:SN:43:ARG:HH12 43:SN:102:GLY:HA3 1.66 0.59 4:3E:87:LEU:HB3 4:3E:107:VAL:HG22 1.83 0.59 3:3D:297:HIS:NE2 3:3D:309:GLU:OE2 2.33 0.59 15:B1:830:ARG:NH2 48:SY:138:GLU:OE2 2.35 0.59 20:BE:434:HIS:CD2 20:BE:441:ARG:HH11 2.21 0.59 23:E1:144:LEU:HD13 23:E1:150:ILE:HG12 1.85 0.59 34:SA:1160:A:H1’ 34:SA:1620:C:H42 1.66 0.59 34:SA:1216:C:O2 34:SA:1446:A:N6 2.35 0.59 12:AE:469:LEU:O 12:AE:473:SER:HB3 2.03 0.59 15:B1:932:LEU:H 15:B1:1006:TRP:HD1 1.49 0.59 16:BA:431:ILE:HG23 16:BA:432:GLN:HG3 1.85 0.59 16:BA:719:VAL:HB 16:BA:744:ARG:HH22 1.68 0.59 23:E1:44:VAL:HG22 23:E1:113:TYR:HB2 1.84 0.59 7:5A:209:G:OP2 7:5A:209:G:N2 2.33 0.59 34:SA:593:U:H4’ 34:SA:595:G:H4’ 1.84 0.59 Continued on next page... Page 77 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 39:SI:150:GLN:HB3 39:SI:181:ILE:HG22 1.84 0.59 47:SX:17:ALA:HA 47:SX:22:LYS:NZ 2.18 0.59 17:BB:867:GLU:O 17:BB:871:GLN:NE2 2.35 0.59 6:3G:34:LYS:HB2 6:3G:101:SER:HB3 1.84 0.59 22:CB:254:LEU:HG 22:CB:270:ILE:HG12 1.84 0.59 22:CB:313:ASN:HD22 22:CB:326:LEU:HB3 1.67 0.59 24:E3:305:ARG:CG 24:E3:306:ALA:N 2.66 0.59 34:SA:484:C:H42 34:SA:504:U:H3 1.48 0.59 3:3D:176:GLN:HG3 4:3E:187:TRP:HB3 1.85 0.59 6:3G:25:GLN:OE1 6:3G:29:ASN:ND2 2.36 0.59 7:5A:163:G:H1 7:5A:232:U:H3 1.50 0.59 13:AF:240:UNK:O 13:AF:252:UNK:HA 2.02 0.59 16:BA:846:TYR:HB3 20:BE:913:GLU:HG2 1.85 0.59 17:BB:359:SER:O 17:BB:391:ARG:NH2 2.36 0.59 19:BD:272:LEU:HD22 19:BD:288:LEU:HD23 1.85 0.59 34:SA:204:G:O6 34:SA:264:G:N2 2.36 0.59 14:AG:526:UNK:O 14:AG:538:UNK:HA 2.02 0.58 16:BA:554:ASP:O 16:BA:556:ARG:NH1 2.36 0.58 24:E3:279:ILE:HG12 24:E3:283:LEU:HD12 1.84 0.58 36:SF:126:VAL:HA 36:SF:141:THR:HA 1.85 0.58 38:SH:188:ARG:NH1 38:SH:191:ARG:CB 2.65 0.58 2:3B:142:ARG:NH2 2:3B:186:ASP:OD2 2.36 0.58 3:3D:36:GLU:OE1 3:3D:37:GLN:NE2 2.36 0.58 23:E2:178:VAL:HA 23:E2:223:GLU:O 2.03 0.58 20:BE:644:THR:HG22 20:BE:651:ILE:HG12 1.85 0.58 21:CA:52:CYS:HA 21:CA:126:LEU:O 2.04 0.58 25:E4:258:UNK:HA 40:SJ:42:ARG:NH1 2.18 0.58 31:R1:143:LYS:NZ 31:R1:252:LYS:HB3 2.17 0.58 16:BA:477:SER:HB2 16:BA:519:LEU:HD12 1.85 0.58 20:BE:78:SER:OG 20:BE:95:GLU:OE2 2.11 0.58 28:MB:124:MET:HG2 28:MB:126:ASN:H 1.67 0.58 28:MB:193:ASN:HB3 28:MB:226:ASN:HB2 1.84 0.58 39:SI:67:LEU:O 39:SI:71:HIS:ND1 2.37 0.58 16:BA:430:ARG:HH12 16:BA:451:ASP:HB2 1.67 0.58 16:BA:540:SER:HA 16:BA:550:VAL:O 2.03 0.58 34:SA:1163:A:N3 34:SA:1613:U:O2’ 2.35 0.58 40:SJ:61:GLU:HG3 40:SJ:62:THR:HG23 1.85 0.58 5:3F:442:ILE:HA 5:3F:472:PRO:HA 1.85 0.58 12:AE:349:THR:O 12:AE:353:ARG:HB2 2.04 0.58 13:AF:69:UNK:O 13:AF:76:UNK:HA 2.03 0.58 15:B1:253:TYR:HA 15:B1:787:SER:O 2.02 0.58 Continued on next page... Page 78 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 16:BA:306:ASN:H 16:BA:321:SER:HA 1.69 0.58 17:BB:565:PHE:O 31:R1:207:ARG:NH2 2.37 0.58 19:BD:272:LEU:HB2 19:BD:288:LEU:HB3 1.85 0.58 38:SH:187:LYS:NZ 38:SH:191:ARG:NH1 2.52 0.58 12:AE:365:ILE:O 12:AE:369:LEU:CB 2.52 0.58 16:BA:247:SER:O 16:BA:249:ARG:NH1 2.37 0.58 18:BC:412:ALA:HB3 18:BC:430:TYR:HB2 1.85 0.58 23:E1:85:SER:OG 23:E1:211:ARG:NH2 2.37 0.58 26:K1:146:VAL:HA 26:K1:153:LEU:HB2 1.84 0.58 26:K1:44:LEU:HD22 35:SC:115:ARG:HH11 1.69 0.58 54:U1:96:UNK:HA 54:U1:112:UNK:HA 1.85 0.58 16:BA:542:PHE:HB3 16:BA:548:LYS:HA 1.86 0.58 19:BD:568:VAL:HA 19:BD:578:ALA:O 2.03 0.58 22:CB:487:ILE:HD12 22:CB:582:GLN:HE21 1.68 0.58 37:SG:51:VAL:HG21 37:SG:131:GLN:HB2 1.85 0.58 48:SY:76:LEU:HD13 48:SY:79:ASN:HD22 1.67 0.58 6:3G:27:ALA:HB1 6:3G:32:GLN:HB3 1.86 0.58 12:AE:333:LEU:O 12:AE:337:ASP:HB2 2.04 0.58 17:BB:454:LEU:O 17:BB:468:ILE:HB 2.03 0.58 31:R1:156:ARG:NH2 31:R1:228:ASP:OD2 2.37 0.58 54:U1:202:UNK:HA 54:U1:208:UNK:HA 1.86 0.58 8:AA:485:UNK:O 8:AA:493:UNK:N 2.37 0.58 12:AE:480:ASN:ND2 12:AE:520:GLU:O 2.36 0.58 15:B1:117:VAL:HB 15:B1:146:VAL:HG12 1.86 0.58 15:B1:119:LEU:HB3 15:B1:147:LEU:O 2.03 0.58 24:E3:231:LYS:NZ 34:SA:1235:C:OP1 2.37 0.58 34:SA:897:C:O2’ 34:SA:914:G:N2 2.37 0.58 2:3B:261:LEU:O 3:3D:129:ARG:NH2 2.37 0.57 2:3C:261:LEU:O 4:3E:118:ARG:NH2 2.37 0.57 5:3F:286:LEU:HB3 5:3F:295:LEU:HD11 1.84 0.57 12:AE:371:LYS:O 12:AE:374:ARG:NH2 2.36 0.57 16:BA:507:GLN:HB2 46:SR:127:LYS:HD3 1.85 0.57 17:BB:341:ALA:HA 17:BB:357:THR:HA 1.86 0.57 17:BB:439:LEU:HD23 17:BB:443:LEU:HB2 1.86 0.57 18:BC:759:THR:O 18:BC:762:CYS:HB3 2.03 0.57 21:CA:183:ASP:O 21:CA:187:HIS:HB2 2.04 0.57 26:K1:42:MET:SD 35:SC:112:SER:OG 2.61 0.57 34:SA:1253:U:H3’ 43:SN:46:ARG:HH22 1.69 0.57 4:3E:289:GLN:O 4:3E:391:ARG:NH2 2.37 0.57 5:3F:537:PHE:O 5:3F:567:VAL:HA 2.04 0.57 6:3G:21:LEU:O 6:3G:25:GLN:HB2 2.03 0.57 Continued on next page... Page 79 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 8:AA:444:UNK:O 8:AA:452:UNK:N 2.37 0.57 17:BB:866:LYS:NZ 18:BC:806:LEU:O 2.36 0.57 18:BC:666:GLU:HG2 18:BC:688:LEU:HD11 1.85 0.57 20:BE:546:ILE:HD13 20:BE:560:LEU:HD13 1.85 0.57 22:CB:532:PRO:HG2 22:CB:693:LYS:HE2 1.85 0.57 23:E2:46:ALA:HA 23:E2:115:GLN:HB3 1.86 0.57 34:SA:1118:G:H2’ 34:SA:1119:G:H8 1.69 0.57 28:MB:181:ASN:HA 34:SA:1623:C:H4’ 1.86 0.57 57:U4:69:THR:HG22 57:U4:73:ASN:HD21 1.70 0.57 7:5A:466:A:H2’ 7:5A:467:A:H8 1.69 0.57 8:AA:433:UNK:O 8:AA:444:UNK:HA 2.05 0.57 20:BE:616:THR:HG23 20:BE:618:GLY:H 1.69 0.57 54:U1:36:UNK:HA 54:U1:47:UNK:HA 1.85 0.57 19:BD:320:TYR:HB3 19:BD:343:SER:HB2 1.87 0.57 3:3D:157:ALA:O 3:3D:161:ALA:HB3 2.04 0.57 12:AE:630:TYR:CZ 12:AE:637:ILE:HG21 2.38 0.57 15:B1:283:LEU:HB3 15:B1:788:TYR:HD2 1.68 0.57 16:BA:126:ASP:OD1 16:BA:128:ASN:ND2 2.38 0.57 16:BA:505:ARG:HD3 34:SA:1616:G:H5’ 1.86 0.57 37:SG:84:LYS:O 37:SG:92:ARG:NH1 2.38 0.57 4:3E:228:PRO:HD2 4:3E:231:ILE:HD12 1.86 0.57 16:BA:153:THR:HG21 16:BA:198:PHE:HA 1.85 0.57 17:BB:554:THR:HG22 17:BB:570:TYR:HB2 1.87 0.57 23:E1:114:ILE:HB 23:E1:122:ILE:HB 1.87 0.57 37:SG:73:THR:OG1 46:SR:114:ARG:NH2 2.37 0.57 39:SI:154:LEU:HD21 39:SI:185:ILE:HG13 1.86 0.57 34:SA:1213:G:N2 34:SA:1450:U:O2 2.35 0.57 34:SA:297:U:H5” 36:SF:37:LYS:HG2 1.85 0.57 5:3F:496:LEU:O 5:3F:513:GLU:HA 2.05 0.57 6:3H:25:GLN:OE1 6:3H:29:ASN:ND2 2.37 0.57 14:AG:7:UNK:HA 14:AG:568:UNK:HA 1.87 0.57 17:BB:230:LYS:HD3 17:BB:237:LYS:HB3 1.87 0.57 19:BD:429:TYR:OH 20:BE:563:ASP:OD1 2.22 0.57 23:E2:42:ILE:O 23:E2:203:CYS:HA 2.05 0.57 25:E4:233:UNK:O 25:E4:240:UNK:HA 2.04 0.57 42:SM:128:CYS:SG 42:SM:129:ARG:N 2.76 0.57 1:3A:3:C:H2’ 1:3A:4:G:H8 1.69 0.57 20:BE:172:HIS:NE2 20:BE:176:TYR:O 2.36 0.57 21:CA:88:LEU:HB2 21:CA:125:LEU:HB2 1.87 0.57 22:CB:527:TYR:HB2 22:CB:615:VAL:HB 1.87 0.57 34:SA:1486:G:OP2 34:SA:1486:G:N2 2.34 0.57 Continued on next page... Page 80 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 37:SG:68:ILE:HG22 46:SR:114:ARG:NH1 2.18 0.57 5:3F:305:ARG:HG2 5:3F:317:ILE:HG12 1.87 0.57 16:BA:585:TYR:HA 16:BA:592:ILE:HG22 1.87 0.56 18:BC:713:GLU:HB3 18:BC:762:CYS:HB2 1.86 0.56 21:CA:185:HIS:O 21:CA:189:ALA:HB2 2.05 0.56 24:E3:344:LYS:NZ 34:SA:1266:U:O2 2.37 0.56 17:BB:499:PHE:HB2 17:BB:518:PRO:HG2 1.87 0.56 3:3D:218:LYS:NZ 3:3D:240:LEU:HD22 2.19 0.56 16:BA:553:ILE:HD13 46:SR:99:GLU:HG2 1.86 0.56 22:CB:344:ASN:HD21 22:CB:445:ASN:HD21 1.51 0.56 23:E2:96:LEU:HD21 23:E2:114:ILE:HD11 1.86 0.56 34:SA:1080:U:O4 34:SA:1081:A:N6 2.38 0.56 5:3F:201:ILE:O 5:3F:538:ARG:NH1 2.38 0.56 5:3F:551:ARG:NH1 6:3G:94:SER:HA 2.21 0.56 12:AE:370:LEU:HD23 12:AE:400:LEU:HD13 1.88 0.56 15:B1:634:ARG:NH1 31:R1:185:ARG:HG2 2.20 0.56 16:BA:430:ARG:HE 16:BA:432:GLN:H 1.53 0.56 19:BD:541:LEU:O 19:BD:542:ARG:NH1 2.36 0.56 20:BE:92:ALA:O 20:BE:98:VAL:HA 2.04 0.56 34:SA:1583:A:H61 34:SA:1611:A:H5” 1.70 0.56 34:SA:1585:U:H3 34:SA:1611:A:H2 1.53 0.56 12:AE:25:ARG:O 12:AE:29:HIS:HB2 2.05 0.56 17:BB:171:CYS:SG 17:BB:172:GLN:N 2.79 0.56 18:BC:548:LEU:O 18:BC:559:ILE:HA 2.04 0.56 20:BE:311:VAL:HG22 20:BE:321:GLU:HG2 1.86 0.56 23:E1:104:ILE:N 23:E1:246:GLU:OE2 2.39 0.56 34:SA:349:U:OP2 42:SM:106:ASN:ND2 2.39 0.56 37:SG:54:LYS:HZ2 37:SG:131:GLN:HE22 1.54 0.56 38:SH:4:ASN:HB3 38:SH:110:ALA:HA 1.88 0.56 40:SJ:9:HIS:O 40:SJ:18:ARG:NH2 2.39 0.56 42:SM:67:ARG:NH1 42:SM:127:GLN:OE1 2.38 0.56 47:SX:75:ILE:HG12 47:SX:127:GLY:HA2 1.86 0.56 5:3F:304:ILE:O 5:3F:317:ILE:HA 2.05 0.56 12:AE:653:PHE:O 12:AE:657:GLN:HB2 2.06 0.56 16:BA:847:ARG:HH22 18:BC:761:ARG:HH11 1.54 0.56 20:BE:601:VAL:HA 20:BE:610:ARG:O 2.06 0.56 28:MB:261:LEU:H 34:SA:571:G:H21 1.54 0.56 7:5A:556:G:H2’ 7:5A:557:A:H8 1.70 0.56 16:BA:799:ILE:O 16:BA:803:LEU:HB3 2.05 0.56 16:BA:799:ILE:O 16:BA:803:LEU:CB 2.54 0.56 20:BE:190:LEU:HB3 20:BE:200:PHE:HB3 1.87 0.56 Continued on next page... Page 81 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 12:AE:33:LEU:HD22 12:AE:150:ARG:HB3 1.86 0.56 24:E3:311:LEU:HD22 24:E3:345:VAL:HB 1.87 0.56 5:3F:289:ARG:NH1 5:3F:334:GLU:HB2 2.21 0.56 16:BA:541:ILE:HG23 16:BA:549:GLN:HB2 1.88 0.56 18:BC:465:LYS:H 18:BC:481:LYS:HD2 1.70 0.56 2:3B:227:PRO:HB2 2:3B:259:MET:HG2 1.87 0.56 19:BD:292:GLY:O 19:BD:318:ARG:NH1 2.39 0.56 23:E2:176:ARG:NH2 23:E2:192:TYR:OH 2.39 0.56 34:SA:337:G:H1’ 34:SA:339:C:OP2 2.05 0.56 15:B1:268:HIS:ND1 15:B1:274:ASP:OD1 2.37 0.55 17:BB:616:ASP:HB3 17:BB:635:LYS:HB3 1.88 0.55 20:BE:128:LEU:HB3 20:BE:140:TYR:HB2 1.87 0.55 35:SC:69:CYS:HA 35:SC:83:LYS:HA 1.88 0.55 58:U5:39:ILE:HB 58:U5:243:PHE:HB2 1.88 0.55 1:3A:82:G:N2 1:3A:327:G:O2’ 2.35 0.55 4:3E:191:HIS:ND1 4:3E:245:THR:O 2.38 0.55 4:3E:95:ALA:O 4:3E:99:ASN:HB2 2.06 0.55 12:AE:671:LEU:O 12:AE:675:ASN:ND2 2.39 0.55 22:CB:970:TRP:O 22:CB:1028:ARG:NH2 2.39 0.55 34:SA:283:U:C2 38:SH:188:ARG:CG 2.89 0.55 34:SA:132:U:O4 56:U3:1015:UNK:N 2.39 0.55 17:BB:899:GLY:O 17:BB:903:GLN:NE2 2.40 0.55 19:BD:576:LEU:HA 19:BD:589:TRP:O 2.06 0.55 21:CA:53:LEU:HB2 21:CA:126:LEU:HB3 1.89 0.55 26:K1:146:VAL:HG22 26:K1:150:GLY:HA2 1.87 0.55 34:SA:1085:G:N1 34:SA:1089:U:O4 2.40 0.55 34:SA:446:A:N1 34:SA:461:G:O2’ 2.35 0.55 46:SR:52:LEU:O 46:SR:59:LYS:NZ 2.38 0.55 46:SR:48:VAL:HG23 46:SR:82:ARG:HB3 1.88 0.55 12:AE:333:LEU:O 12:AE:337:ASP:CB 2.54 0.55 19:BD:520:ASN:HD22 19:BD:533:ALA:HB3 1.72 0.55 23:E1:89:PRO:HG2 23:E1:134:PHE:HZ 1.71 0.55 31:R1:311:LEU:HB3 31:R1:352:ALA:HB3 1.87 0.55 18:BC:216:ARG:NH1 35:SC:25:THR:O 2.39 0.55 36:SF:59:ARG:HG3 36:SF:62:LYS:NZ 2.22 0.55 6:3H:34:LYS:O 6:3H:100:ALA:HA 2.07 0.55 12:AE:141:ASN:ND2 12:AE:209:GLN:OE1 2.40 0.55 16:BA:17:GLN:NE2 16:BA:306:ASN:OD1 2.40 0.55 17:BB:509:VAL:HG13 17:BB:515:LYS:HE2 1.88 0.55 5:3F:440:HIS:HD1 5:3F:493:SER:HG 1.55 0.55 17:BB:16:VAL:HG13 17:BB:18:ALA:H 1.71 0.55 Continued on next page... Page 82 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 22:CB:494:GLU:OE1 22:CB:497:ARG:NH2 2.38 0.55 25:E4:55:UNK:HA 25:E4:71:UNK:HA 1.88 0.55 36:SF:59:ARG:HA 36:SF:62:LYS:NZ 2.21 0.55 16:BA:603:LEU:HB3 16:BA:611:LEU:HB2 1.88 0.55 34:SA:1498:G:H4’ 58:U5:250:THR:HA 1.89 0.55 16:BA:117:ARG:NH2 16:BA:145:HIS:O 2.40 0.55 17:BB:640:LYS:HG2 17:BB:652:LYS:HG3 1.89 0.55 18:BC:270:ILE:HG22 18:BC:277:VAL:HG12 1.86 0.55 19:BD:524:SER:HB3 19:BD:529:ILE:HB 1.89 0.55 34:SA:483:A:N6 34:SA:504:U:O4 2.40 0.55 48:SY:97:ASP:HB2 48:SY:100:ASP:OD2 2.06 0.55 49:SZ:32:ARG:NH1 49:SZ:35:VAL:HG23 2.22 0.55 57:U4:115:ILE:HA 57:U4:118:LYS:HE2 1.87 0.55 3:3D:323:THR:HG22 3:3D:327:LYS:NZ 2.22 0.55 8:AA:79:UNK:N 8:AA:83:UNK:O 2.40 0.55 12:AE:661:ILE:HG12 12:AE:663:SER:H 1.71 0.55 22:CB:1100:VAL:HB 22:CB:1182:ILE:HB 1.88 0.55 26:K1:198:TYR:O 26:K1:202:GLU:CB 2.54 0.55 31:R1:66:GLU:HB2 31:R1:75:ILE:HB 1.89 0.55 39:SI:137:GLY:H 39:SI:153:LEU:HB2 1.71 0.55 46:SR:25:GLY:HA3 46:SR:64:ASP:OD2 2.07 0.55 16:BA:10:LEU:HA 16:BA:702:LEU:H 1.70 0.54 17:BB:391:ARG:HG3 17:BB:392:THR:HG23 1.89 0.54 34:SA:151:G:N3 38:SH:13:GLN:NE2 2.55 0.54 36:SF:176:ASP:HB3 36:SF:179:LYS:NZ 2.23 0.54 23:E2:31:LEU:HD13 23:E2:40:ARG:HE 1.72 0.54 46:SR:10:PHE:HB2 46:SR:12:LYS:NZ 2.23 0.54 47:SX:118:ARG:HH12 47:SX:119:LYS:NZ 2.05 0.54 1:3A:86:A:N3 4:3E:336:THR:OG1 2.38 0.54 2:3C:198:GLU:HG3 2:3C:200:SER:H 1.73 0.54 24:E3:378:LYS:HD2 34:SA:1220:C:H4’ 1.89 0.54 34:SA:442:C:O2’ 34:SA:526:A:N6 2.41 0.54 15:B1:149:VAL:HG22 15:B1:184:PHE:HB2 1.90 0.54 20:BE:272:ASP:HA 20:BE:287:LEU:O 2.06 0.54 21:CA:18:PHE:HB2 21:CA:35:HIS:HB3 1.90 0.54 23:E1:31:LEU:HD22 23:E1:40:ARG:HE 1.72 0.54 31:R1:11:PHE:HB3 31:R1:20:ARG:NH1 2.22 0.54 34:SA:1610:G:H5” 37:SG:107:LYS:HE3 1.88 0.54 44:SO:20:ARG:HH21 47:SX:56:HIS:HB3 1.71 0.54 2:3C:319:ARG:HD2 2:3C:322:ARG:HD3 1.90 0.54 12:AE:802:PHE:O 12:AE:806:LEU:HB2 2.08 0.54 Continued on next page... Page 83 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 15:B1:857:LEU:HD13 15:B1:1012:LYS:HD3 1.88 0.54 18:BC:310:THR:HB 18:BC:363:ARG:HH12 1.70 0.54 33:S1:209:UNK:HA 33:S1:219:UNK:HA 1.88 0.54 34:SA:955:A:H5” 44:SO:10:GLY:HA3 1.88 0.54 54:U1:109:UNK:HA 54:U1:119:UNK:HA 1.89 0.54 12:AE:367:LEU:HA 12:AE:400:LEU:HD11 1.88 0.54 18:BC:467:ILE:H 18:BC:480:ILE:HG21 1.71 0.54 28:MB:192:ASP:HB2 28:MB:225:ALA:HA 1.89 0.54 34:SA:1685:G:N2 34:SA:1716:C:N3 2.56 0.54 36:SF:104:ASP:N 36:SF:108:ARG:O 2.40 0.54 19:BD:237:ARG:NH1 19:BD:238:LEU:O 2.40 0.54 19:BD:576:LEU:HB3 19:BD:588:LEU:HD12 1.90 0.54 20:BE:131:SER:HB3 20:BE:170:LEU:HD11 1.89 0.54 23:E1:150:ILE:O 23:E1:158:LYS:HA 2.08 0.54 27:MA:138:VAL:HG22 27:MA:158:VAL:HG22 1.88 0.54 40:SJ:72:ILE:HD12 40:SJ:74:LYS:NZ 2.22 0.54 13:AF:195:UNK:O 60:UB:394:UNK:N 2.41 0.54 14:AG:346:UNK:HA 14:AG:353:UNK:HA 1.89 0.54 23:E1:179:THR:HB 23:E1:224:LYS:HG2 1.88 0.54 34:SA:1218:G:OP1 34:SA:1444:A:N6 2.40 0.54 35:SC:87:ARG:HH11 35:SC:99:ASN:HD22 1.53 0.54 38:SH:188:ARG:CZ 38:SH:191:ARG:CD 2.70 0.54 58:U5:128:ASP:HB3 58:U5:131:VAL:HG22 1.88 0.54 15:B1:931:LYS:HD3 15:B1:1004:ARG:HB3 1.90 0.54 16:BA:792:ILE:O 16:BA:796:LEU:HB2 2.08 0.54 19:BD:237:ARG:NH2 19:BD:240:ASP:OD1 2.41 0.54 24:E3:413:LEU:HG 24:E3:447:ARG:HH21 1.73 0.54 34:SA:273:G:O6 34:SA:283:U:O2 2.24 0.54 38:SH:187:LYS:NZ 38:SH:191:ARG:HH12 2.05 0.54 58:U5:71:ASN:HB2 58:U5:122:PHE:HA 1.90 0.54 14:AG:487:UNK:O 14:AG:495:UNK:N 2.41 0.54 17:BB:538:ARG:NH1 17:BB:539:VAL:O 2.40 0.54 23:E1:111:GLN:NE2 23:E1:113:TYR:OH 2.41 0.54 25:E4:160:UNK:O 25:E4:168:UNK:N 2.41 0.54 25:E4:272:UNK:O 25:E4:283:UNK:HA 2.08 0.54 33:S1:221:UNK:N 33:S1:225:UNK:O 2.40 0.54 54:U1:150:UNK:HA 54:U1:160:UNK:HA 1.89 0.54 2:3C:252:ILE:O 2:3C:256:ASN:ND2 2.40 0.53 14:AG:97:UNK:HA 14:AG:113:UNK:HA 1.90 0.53 17:BB:530:LEU:HB3 17:BB:551:LEU:HB3 1.90 0.53 20:BE:62:ASP:OD2 20:BE:65:THR:OG1 2.25 0.53 Continued on next page... Page 84 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:1163:A:H4’ 37:SG:166:ARG:NH1 2.23 0.53 42:SM:3:THR:OG1 42:SM:4:GLU:N 2.41 0.53 1:3A:83:A:H61 1:3A:327:G:H1’ 1.73 0.53 8:AA:394:UNK:O 8:AA:401:UNK:HA 2.08 0.53 17:BB:536:CYS:H 17:BB:549:SER:HB2 1.73 0.53 20:BE:60:ILE:HB 20:BE:70:PHE:HB2 1.89 0.53 22:CB:162:PHE:HB2 22:CB:597:ARG:HD2 1.89 0.53 27:MA:70:PHE:O 27:MA:74:HIS:ND1 2.40 0.53 28:MB:167:LEU:HA 28:MB:256:MET:HG2 1.90 0.53 24:E3:301:TYR:OH 34:SA:1217:A:C2 2.55 0.53 34:SA:1175:U:O4 34:SA:1464:G:O6 2.25 0.53 34:SA:485:A:H1’ 34:SA:503:G:H22 1.73 0.53 40:SJ:101:ILE:HD12 40:SJ:184:LEU:HD21 1.89 0.53 44:SO:119:GLU:O 44:SO:123:HIS:ND1 2.38 0.53 4:3E:68:VAL:HG13 4:3E:72:LEU:HD22 1.90 0.53 5:3F:190:VAL:HA 5:3F:195:GLN:O 2.08 0.53 6:3G:53:ILE:HB 6:3G:100:ALA:HB3 1.89 0.53 9:AB:259:UNK:O 9:AB:277:UNK:N 2.41 0.53 12:AE:630:TYR:OH 12:AE:637:ILE:CD1 2.35 0.53 19:BD:530:LEU:HB3 19:BD:544:VAL:HB 1.90 0.53 21:CA:103:LEU:O 22:CB:869:ASN:ND2 2.38 0.53 23:E2:104:ILE:N 23:E2:246:GLU:OE2 2.41 0.53 49:SZ:29:HIS:ND1 49:SZ:32:ARG:O 2.37 0.53 54:U1:197:UNK:O 54:U1:215:UNK:N 2.41 0.53 2:3B:171:LEU:HB3 2:3B:240:VAL:HG23 1.91 0.53 7:5A:403:C:OP2 7:5A:404:G:O2’ 2.17 0.53 15:B1:822:PHE:HA 15:B1:888:PRO:HA 1.91 0.53 18:BC:510:SER:OG 18:BC:512:ASP:OD1 2.25 0.53 33:S1:208:UNK:N 33:S1:220:UNK:O 2.42 0.53 33:S1:290:UNK:N 33:S1:302:UNK:O 2.41 0.53 44:SO:111:ALA:HA 44:SO:114:ARG:HB2 1.90 0.53 46:SR:50:GLU:OE1 46:SR:82:ARG:NH2 2.41 0.53 3:3D:223:ILE:HG22 3:3D:225:ASP:H 1.74 0.53 16:BA:328:LEU:HG 16:BA:343:GLY:HA2 1.90 0.53 18:BC:533:LYS:HB2 18:BC:554:ASP:HB3 1.90 0.53 18:BC:576:ASN:HD22 18:BC:596:ASP:HA 1.74 0.53 20:BE:418:ALA:HB3 20:BE:431:ILE:HB 1.89 0.53 22:CB:751:LYS:N 22:CB:785:GLU:O 2.41 0.53 1:3A:7:G:O6 34:SA:1117:U:O4 2.27 0.53 12:AE:478:THR:HA 12:AE:490:ARG:HD2 1.90 0.53 12:AE:566:CYS:O 12:AE:570:ALA:HB2 2.09 0.53 Continued on next page... Page 85 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 15:B1:943:LYS:HD2 34:SA:573:C:OP2 2.09 0.53 16:BA:131:ARG:HG3 20:BE:27:PHE:HA 1.91 0.53 22:CB:797:THR:HG23 22:CB:836:GLU:HG3 1.90 0.53 23:E1:103:PRO:HG3 23:E2:232:LEU:HD21 1.90 0.53 24:E3:378:LYS:O 34:SA:1220:C:O2’ 2.25 0.53 34:SA:38:C:O2’ 34:SA:470:A:N6 2.42 0.53 35:SC:144:ARG:NH2 35:SC:207:LEU:O 2.39 0.53 40:SJ:79:ALA:HB3 40:SJ:164:ARG:HH11 1.73 0.53 12:AE:354:SER:O 12:AE:357:ARG:NH2 2.42 0.53 12:AE:78:SER:HB2 12:AE:125:PHE:HZ 1.73 0.53 17:BB:38:PRO:HD2 17:BB:41:LEU:HB2 1.91 0.53 20:BE:526:VAL:O 20:BE:539:LYS:HA 2.09 0.53 33:S1:220:UNK:HA 33:S1:226:UNK:HA 1.90 0.53 35:SC:223:PHE:HD2 35:SC:225:VAL:HG23 1.74 0.53 36:SF:54:TYR:HB3 49:SZ:17:LEU:HD11 1.91 0.53 1:3A:18:G:H2’ 1:3A:19:A:C8 2.44 0.53 5:3F:287:ALA:HB3 5:3F:296:TYR:HB2 1.91 0.53 7:5A:174:U:O4 7:5A:222:G:O6 2.26 0.53 7:5A:534:A:OP2 7:5A:534:A:H8 1.92 0.53 17:BB:165:SER:HB2 17:BB:182:LYS:HD2 1.91 0.53 17:BB:478:SER:O 17:BB:491:GLY:N 2.41 0.53 17:BB:559:PHE:HE1 17:BB:564:LYS:HB2 1.74 0.53 17:BB:553:ASN:HB3 17:BB:572:HIS:HB2 1.91 0.53 18:BC:16:TYR:HB3 18:BC:641:PHE:H 1.74 0.53 20:BE:459:ASP:O 20:BE:481:ASN:ND2 2.42 0.53 22:CB:578:ILE:HA 22:CB:620:ASN:H 1.74 0.53 31:R1:202:PRO:HG3 31:R1:229:VAL:HG11 1.91 0.53 33:S1:342:UNK:O 33:S1:350:UNK:N 2.42 0.53 34:SA:1663:G:O6 34:SA:1738:U:O4 2.27 0.53 34:SA:478:A:O2’ 41:SK:124:HIS:ND1 2.42 0.53 34:SA:298:C:H5” 36:SF:38:LEU:HB2 1.90 0.53 40:SJ:8:ARG:NE 40:SJ:19:ALA:O 2.40 0.53 47:SX:18:GLU:HG3 47:SX:69:LEU:HD12 1.91 0.53 54:U1:61:UNK:N 54:U1:66:UNK:O 2.41 0.53 18:BC:174:VAL:HB 18:BC:184:HIS:HB2 1.90 0.53 34:SA:1042:G:H22 34:SA:1076:A:H2 1.57 0.53 34:SA:1647:U:H2’ 34:SA:1648:A:H8 1.74 0.53 34:SA:299:A:OP2 36:SF:38:LEU:HG 2.09 0.53 1:3A:66:U:H2’ 1:3A:67:G:H8 1.73 0.53 5:3F:297:ALA:HB3 5:3F:305:ARG:O 2.09 0.53 7:5A:545:G:OP1 7:5A:547:C:N4 2.42 0.53 Continued on next page... Page 86 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 16:BA:36:ARG:HG2 16:BA:52:TYR:HB2 1.91 0.53 17:BB:898:LEU:HD11 18:BC:789:THR:HB 1.91 0.53 20:BE:275:PHE:O 20:BE:284:ILE:N 2.42 0.53 21:CA:46:ASN:HB3 21:CA:49:GLU:HB2 1.91 0.53 22:CB:174:TYR:HB3 22:CB:223:ARG:NH1 2.24 0.53 25:E4:190:UNK:O 25:E4:201:UNK:HA 2.09 0.53 34:SA:1216:C:H4’ 34:SA:1217:A:OP1 2.09 0.53 36:SF:112:HIS:NE2 36:SF:237:SER:OG 2.38 0.53 34:SA:1611:A:O2’ 37:SG:95:ASN:O 2.26 0.52 34:SA:865:A:OP1 47:SX:28:ARG:NH1 2.43 0.52 49:SZ:92:VAL:HG13 49:SZ:99:LYS:HD2 1.90 0.52 24:E3:418:HIS:O 24:E3:422:LEU:CB 2.57 0.52 34:SA:1217:A:H2’ 34:SA:1217:A:N3 2.24 0.52 54:U1:19:UNK:HA 54:U1:26:UNK:HA 1.90 0.52 2:3B:271:ILE:HB 2:3B:314:CYS:O 2.08 0.52 12:AE:105:ALA:O 12:AE:109:TRP:NE1 2.42 0.52 17:BB:185:MET:HA 17:BB:201:ILE:HG22 1.90 0.52 17:BB:426:ARG:HD2 17:BB:457:PHE:HB3 1.91 0.52 18:BC:257:ILE:HG12 18:BC:299:ASN:HD22 1.74 0.52 24:E3:208:GLU:O 24:E3:209:LYS:HE2 2.09 0.52 33:S1:180:UNK:N 33:S1:184:UNK:O 2.43 0.52 34:SA:354:C:H5’ 40:SJ:14:THR:HG21 1.92 0.52 41:SK:53:ARG:NH2 41:SK:97:LEU:O 2.41 0.52 2:3B:88:ILE:HD13 2:3B:107:VAL:HG21 1.90 0.52 23:E1:112:VAL:O 23:E1:124:VAL:HB 2.08 0.52 1:3A:66:U:H3 7:5A:287:G:H1 1.57 0.52 8:AA:382:UNK:N 8:AA:395:UNK:O 2.42 0.52 9:AB:4:UNK:HA 9:AB:283:UNK:O 2.09 0.52 18:BC:346:VAL:HB 18:BC:352:LYS:HB3 1.92 0.52 23:E2:178:VAL:HG12 23:E2:223:GLU:HB3 1.91 0.52 31:R1:176:GLN:HG3 31:R1:305:LYS:HB3 1.92 0.52 28:MB:143:HIS:HB2 34:SA:1604:U:H5” 1.91 0.52 38:SH:2:LYS:HB3 38:SH:108:VAL:HG22 1.92 0.52 34:SA:404:G:O2’ 38:SH:88:ARG:NH1 2.42 0.52 39:SI:62:VAL:HG12 39:SI:64:VAL:H 1.74 0.52 42:SM:97:TYR:HB3 42:SM:99:ARG:HH11 1.74 0.52 48:SY:107:PHE:HE1 48:SY:123:LYS:HB3 1.74 0.52 7:5A:242:C:H2’ 7:5A:243:A:H8 1.74 0.52 12:AE:316:PRO:HA 12:AE:319:ILE:HG22 1.91 0.52 16:BA:206:ILE:O 16:BA:217:VAL:HA 2.09 0.52 16:BA:60:ALA:HB3 16:BA:73:ILE:HB 1.91 0.52 Continued on next page... Page 87 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 18:BC:691:PRO:HG3 18:BC:742:ARG:HE 1.74 0.52 34:SA:25:C:N4 34:SA:470:A:OP1 2.43 0.52 42:SM:33:ARG:HH11 42:SM:61:THR:HG21 1.75 0.52 2:3B:122:ARG:HG2 2:3B:142:ARG:HG2 1.91 0.52 7:5A:178:G:O6 7:5A:219:U:O2 2.26 0.52 16:BA:92:HIS:HD2 16:BA:135:PRO:HD2 1.74 0.52 19:BD:575:GLY:O 19:BD:591:LEU:N 2.43 0.52 23:E2:211:ARG:HH12 34:SA:1190:C:H3’ 1.75 0.52 34:SA:283:U:C4 38:SH:188:ARG:CB 2.73 0.52 34:SA:361:C:H3’ 34:SA:378:A:OP2 2.09 0.52 39:SI:161:GLN:HG2 39:SI:162:ILE:HG13 1.92 0.52 57:U4:66:LEU:N 57:U4:152:ILE:O 2.42 0.52 22:CB:904:HIS:O 22:CB:907:GLN:NE2 2.42 0.52 37:SG:80:LYS:HB2 37:SG:83:ARG:NH1 2.24 0.52 4:3E:125:PRO:HB3 4:3E:132:SER:HA 1.91 0.52 4:3E:249:GLN:HA 4:3E:252:LEU:HD12 1.92 0.52 17:BB:456:LEU:HB3 17:BB:466:ASP:O 2.10 0.52 20:BE:471:CYS:SG 20:BE:473:ASN:ND2 2.83 0.52 20:BE:641:LEU:HB3 20:BE:654:TRP:HB2 1.91 0.52 23:E1:188:ARG:HD3 23:E1:191:ASP:OD2 2.10 0.52 26:K1:61:THR:HA 26:K1:64:LEU:HB3 1.92 0.52 32:R2:1509:GLU:HB3 32:R2:1512:GLU:HB3 1.91 0.52 34:SA:297:U:OP1 36:SF:37:LYS:NZ 2.42 0.52 38:SH:4:ASN:HA 38:SH:15:THR:HG22 1.92 0.52 12:AE:478:THR:HG22 12:AE:490:ARG:HB3 1.91 0.52 18:BC:211:LEU:O 18:BC:223:TRP:HB2 2.10 0.52 19:BD:302:THR:HG21 19:BD:364:GLN:HB3 1.92 0.52 21:CA:169:VAL:HG23 21:CA:173:LYS:NZ 2.25 0.52 22:CB:748:LEU:HD21 22:CB:791:LYS:HD2 1.92 0.52 26:K1:159:LEU:HD13 26:K1:203:LEU:HB2 1.92 0.52 26:K1:61:THR:O 26:K1:65:ASP:HB3 2.10 0.52 35:SC:141:ALA:HA 35:SC:210:ILE:HG12 1.91 0.52 43:SN:42:ALA:HB3 43:SN:122:VAL:HB 1.92 0.52 37:SG:76:ARG:HH11 46:SR:120:ASP:CG 2.13 0.52 6:3G:24:VAL:HG22 6:3G:102:ILE:HD11 1.93 0.51 6:3H:106:ASP:O 6:3H:111:LYS:NZ 2.33 0.51 18:BC:122:THR:HG23 18:BC:124:GLY:H 1.75 0.51 19:BD:406:ASP:OD1 20:BE:371:LYS:NZ 2.43 0.51 33:S1:118:UNK:O 33:S1:127:UNK:N 2.43 0.51 35:SC:107:THR:HB 35:SC:111:ARG:NH1 2.24 0.51 42:SM:127:GLN:NE2 42:SM:128:CYS:O 2.42 0.51 Continued on next page... Page 88 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 2:3B:188:VAL:HG22 2:3B:192:GLY:HA3 1.91 0.51 5:3F:254:GLY:HA3 5:3F:260:LEU:HD23 1.93 0.51 9:AB:59:UNK:N 9:AB:68:UNK:O 2.43 0.51 17:BB:624:LEU:HB2 17:BB:629:ASN:H 1.75 0.51 20:BE:412:PRO:HD2 20:BE:441:ARG:HH12 1.75 0.51 22:CB:127:PHE:HD1 22:CB:283:TYR:HB3 1.75 0.51 22:CB:165:PRO:HD2 22:CB:222:PHE:HE2 1.75 0.51 36:SF:59:ARG:HG3 36:SF:62:LYS:HZ3 1.74 0.51 39:SI:67:LEU:HG 39:SI:71:HIS:HE1 1.75 0.51 14:AG:36:UNK:O 14:AG:48:UNK:HA 2.10 0.51 16:BA:161:ILE:HB 16:BA:173:TRP:O 2.10 0.51 21:CA:134:ILE:O 21:CA:138:TRP:CB 2.57 0.51 28:MB:233:VAL:HB 28:MB:254:PHE:HB2 1.93 0.51 34:SA:1157:A:N6 34:SA:1618:C:O2 2.42 0.51 26:K1:130:ASN:ND2 34:SA:930:A:OP1 2.42 0.51 40:SJ:11:ARG:HH12 40:SJ:17:LYS:HB2 1.75 0.51 44:SO:18:TYR:O 47:SX:56:HIS:NE2 2.43 0.51 37:SG:38:THR:HG21 46:SR:57:LEU:HB3 1.91 0.51 5:3F:365:PRO:O 5:3F:368:LEU:HB2 2.10 0.51 7:5A:21:A:H2’ 7:5A:22:A:H8 1.74 0.51 13:AF:4:UNK:HA 13:AF:284:UNK:HA 1.93 0.51 20:BE:611:THR:HB 20:BE:621:ASP:HB3 1.93 0.51 22:CB:99:LYS:O 22:CB:103:PHE:CB 2.57 0.51 34:SA:1175:U:O2 34:SA:1464:G:N2 2.42 0.51 34:SA:485:A:H3’ 34:SA:486:G:H21 1.76 0.51 39:SI:46:ILE:HD11 39:SI:58:LEU:HB3 1.91 0.51 5:3F:358:THR:O 5:3F:430:LYS:NZ 2.34 0.51 16:BA:142:HIS:NE2 16:BA:174:SER:O 2.40 0.51 17:BB:188:LEU:HD12 17:BB:198:GLU:HB3 1.93 0.51 31:R1:199:ARG:NH2 31:R1:236:GLY:O 2.38 0.51 33:S1:320:UNK:N 33:S1:334:UNK:O 2.44 0.51 34:SA:265:A:OP2 38:SH:194:LYS:NZ 2.29 0.51 34:SA:931:C:H5” 35:SC:116:LYS:HG2 1.93 0.51 35:SC:87:ARG:HH11 35:SC:99:ASN:ND2 2.08 0.51 3:3D:62:ALA:O 3:3D:66:ALA:HB2 2.10 0.51 18:BC:599:ILE:O 18:BC:613:LEU:HB2 2.10 0.51 19:BD:581:ASN:OD1 19:BD:585:LYS:N 2.43 0.51 20:BE:33:VAL:N 20:BE:317:ASN:OD1 2.41 0.51 22:CB:1222:GLU:O 22:CB:1226:PHE:HB2 2.11 0.51 33:S1:178:UNK:O 33:S1:186:UNK:N 2.43 0.51 7:5A:357:G:O6 7:5A:368:U:O2 2.28 0.51 Continued on next page... Page 89 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 14:AG:528:UNK:O 14:AG:536:UNK:N 2.44 0.51 16:BA:165:SER:OG 16:BA:166:LYS:N 2.43 0.51 16:BA:163:THR:O 16:BA:170:ALA:HA 2.11 0.51 17:BB:225:GLN:HG2 17:BB:243:THR:HA 1.93 0.51 22:CB:186:ILE:HG22 22:CB:346:LEU:HD22 1.93 0.51 22:CB:842:ILE:O 22:CB:849:GLY:HA2 2.10 0.51 23:E2:174:LYS:HE2 23:E2:200:GLU:HB2 1.92 0.51 31:R1:201:SER:HB3 31:R1:204:LEU:HD13 1.93 0.51 31:R1:96:VAL:HG22 31:R1:122:THR:HA 1.92 0.51 34:SA:1217:A:C2’ 34:SA:1217:A:N3 2.73 0.51 34:SA:512:A:OP2 41:SK:172:VAL:HG13 2.10 0.51 35:SC:134:VAL:HB 35:SC:219:LYS:HB3 1.92 0.51 45:SP:85:ALA:H 45:SP:119:THR:HG22 1.75 0.51 46:SR:39:VAL:HG22 46:SR:41:PRO:HD2 1.92 0.51 3:3D:180:LEU:HD13 4:3E:183:ARG:HG2 1.93 0.51 10:AC:603:UNK:O 10:AC:607:UNK:CB 2.59 0.51 12:AE:288:ALA:HB3 12:AE:326:LYS:HB3 1.93 0.51 12:AE:43:GLN:HE21 12:AE:47:PHE:HB2 1.75 0.51 14:AG:117:UNK:HA 14:AG:131:UNK:O 2.11 0.51 16:BA:39:VAL:HB 16:BA:48:PHE:HB2 1.92 0.51 16:BA:78:ARG:HB2 16:BA:93:PHE:HA 1.91 0.51 18:BC:47:PRO:HD2 18:BC:51:LYS:H 1.76 0.51 19:BD:383:TRP:HZ3 19:BD:397:GLY:HA3 1.75 0.51 36:SF:85:GLY:N 36:SF:88:ASP:OD2 2.40 0.51 37:SG:156:ARG:HH12 37:SG:158:GLN:HB2 1.76 0.51 41:SK:83:VAL:O 41:SK:107:ARG:NE 2.39 0.51 3:3D:107:PRO:O 3:3D:111:GLU:HB2 2.11 0.51 3:3D:323:THR:HG22 3:3D:327:LYS:HZ2 1.74 0.51 5:3F:345:THR:HA 5:3F:359:PHE:O 2.10 0.51 6:3G:33:LEU:HA 6:3G:101:SER:O 2.11 0.51 8:AA:161:UNK:N 8:AA:165:UNK:O 2.44 0.51 8:AA:5:UNK:N 8:AA:565:UNK:O 2.44 0.51 12:AE:303:PHE:HB3 12:AE:346:LYS:HB3 1.92 0.51 12:AE:695:TYR:OH 12:AE:699:ARG:NH2 2.44 0.51 16:BA:752:ASN:O 16:BA:756:GLU:HB2 2.10 0.51 22:CB:215:GLU:N 22:CB:218:ASP:OD2 2.43 0.51 22:CB:738:TYR:O 22:CB:742:PHE:CB 2.59 0.51 23:E2:112:VAL:O 23:E2:124:VAL:HB 2.10 0.51 33:S1:301:UNK:O 33:S1:309:UNK:N 2.44 0.51 47:SX:118:ARG:NH1 47:SX:119:LYS:HZ1 2.09 0.51 57:U4:64:GLN:HA 57:U4:95:ASN:HB3 1.92 0.51 Continued on next page... Page 90 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 5:3F:200:ASP:HB3 5:3F:209:LYS:HB2 1.93 0.51 16:BA:37:VAL:HB 16:BA:51:GLU:HB2 1.92 0.51 17:BB:629:ASN:ND2 17:BB:642:TRP:O 2.44 0.51 17:BB:674:SER:H 17:BB:681:ILE:HG23 1.76 0.51 34:SA:913:G:H5” 34:SA:914:G:H5’ 1.92 0.51 36:SF:212:ASP:OD1 36:SF:216:ASN:N 2.43 0.51 45:SP:17:ALA:HA 45:SP:30:VAL:HA 1.92 0.51 6:3H:34:LYS:HB2 6:3H:101:SER:HB3 1.92 0.50 12:AE:572:ARG:N 12:AE:630:TYR:CE2 2.79 0.50 13:AF:221:UNK:O 13:AF:234:UNK:N 2.44 0.50 14:AG:111:UNK:CB 14:AG:119:UNK:O 2.59 0.50 19:BD:352:GLN:HG3 19:BD:371:VAL:HG13 1.92 0.50 22:CB:649:TRP:HB2 22:CB:653:SER:HB2 1.93 0.50 34:SA:548:G:H2’ 34:SA:549:G:H8 1.76 0.50 34:SA:863:A:N7 34:SA:964:U:O4 2.44 0.50 54:U1:117:UNK:N 54:U1:130:UNK:O 2.44 0.50 12:AE:750:ASN:O 12:AE:754:GLU:CB 2.59 0.50 17:BB:180:THR:HG22 17:BB:186:ILE:HG23 1.92 0.50 22:CB:235:LEU:O 22:CB:239:LEU:CB 2.60 0.50 28:MB:215:LYS:HZ2 34:SA:1625:C:H5’ 1.76 0.50 35:SC:190:PRO:HG2 35:SC:192:VAL:HG23 1.93 0.50 48:SY:54:LEU:HD12 48:SY:82:LYS:HD3 1.93 0.50 8:AA:506:UNK:HA 8:AA:516:UNK:O 2.11 0.50 12:AE:333:LEU:HD11 12:AE:369:LEU:HD12 1.92 0.50 12:AE:630:TYR:CD1 12:AE:637:ILE:HD12 2.31 0.50 16:BA:28:GLN:HA 16:BA:41:ASP:HA 1.92 0.50 16:BA:87:ARG:HH11 16:BA:89:VAL:HG22 1.75 0.50 20:BE:135:ASN:HB3 20:BE:159:VAL:HB 1.93 0.50 22:CB:334:PHE:O 22:CB:338:SER:CB 2.59 0.50 36:SF:48:LEU:HD11 36:SF:61:VAL:HG22 1.94 0.50 47:SX:103:ILE:HA 47:SX:112:ASP:HA 1.93 0.50 48:SY:76:LEU:HD12 48:SY:81:LYS:HB2 1.93 0.50 8:AA:425:UNK:O 8:AA:434:UNK:N 2.44 0.50 9:AB:203:UNK:N 9:AB:207:UNK:O 2.45 0.50 15:B1:840:LYS:HG2 15:B1:874:TYR:HB3 1.94 0.50 15:B1:978:ARG:HB3 15:B1:988:ARG:HE 1.77 0.50 19:BD:448:LYS:HE2 20:BE:563:ASP:HB3 1.93 0.50 19:BD:522:GLN:HB2 19:BD:531:CYS:HB3 1.92 0.50 22:CB:861:ILE:O 22:CB:865:ARG:CB 2.59 0.50 24:E3:352:SER:O 24:E3:356:LEU:HB2 2.12 0.50 15:B1:988:ARG:NH1 28:MB:146:ARG:HH11 2.06 0.50 Continued on next page... Page 91 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:1083:G:H1 34:SA:1090:C:H42 1.58 0.50 40:SJ:43:ILE:HA 40:SJ:57:ALA:HA 1.93 0.50 2:3B:173:LEU:HB2 2:3B:242:ALA:HA 1.94 0.50 12:AE:384:ILE:HG23 12:AE:401:VAL:HG22 1.93 0.50 16:BA:168:LEU:O 16:BA:188:ASN:ND2 2.44 0.50 17:BB:125:THR:HG21 17:BB:165:SER:HB3 1.92 0.50 20:BE:438:LYS:NZ 20:BE:460:ASP:OD1 2.45 0.50 20:BE:485:THR:HG22 20:BE:497:LYS:HG3 1.92 0.50 30:P1:186:GLU:HG2 30:P1:188:LYS:H 1.76 0.50 46:SR:58:ASP:N 46:SR:58:ASP:OD1 2.41 0.50 12:AE:769:LEU:O 12:AE:773:GLN:NE2 2.45 0.50 15:B1:931:LYS:HB3 15:B1:1004:ARG:HE 1.76 0.50 21:CA:192:GLU:O 21:CA:196:ALA:CB 2.59 0.50 22:CB:309:LEU:O 22:CB:333:ASN:ND2 2.44 0.50 22:CB:333:ASN:O 22:CB:337:LEU:HB3 2.12 0.50 22:CB:367:ARG:O 22:CB:371:GLN:CB 2.58 0.50 22:CB:93:GLU:O 22:CB:97:LEU:HB2 2.12 0.50 24:E3:267:VAL:HG11 24:E3:310:SER:HB2 1.93 0.50 28:MB:86:PRO:O 28:MB:113:ASN:ND2 2.42 0.50 31:R1:11:PHE:HB2 31:R1:33:ILE:HG12 1.93 0.50 34:SA:1648:A:H2’ 34:SA:1649:G:H8 1.76 0.50 34:SA:327:U:OP1 42:SM:82:ARG:NH1 2.44 0.50 38:SH:114:VAL:HG12 38:SH:115:LYS:HD3 1.93 0.50 42:SM:94:ILE:HG22 42:SM:97:TYR:H 1.76 0.50 58:U5:10:SER:OG 58:U5:217:GLN:NE2 2.41 0.50 12:AE:218:ILE:HG23 12:AE:263:VAL:HG11 1.92 0.50 16:BA:149:ILE:HD13 16:BA:165:SER:HB2 1.93 0.50 16:BA:751:ILE:O 16:BA:755:TYR:HB3 2.11 0.50 18:BC:80:SER:HB3 18:BC:84:LEU:HB2 1.94 0.50 19:BD:418:LYS:HB2 19:BD:422:ILE:HD11 1.93 0.50 26:K1:44:LEU:HD22 35:SC:115:ARG:NH1 2.25 0.50 34:SA:1661:U:H3 34:SA:1740:A:H61 1.59 0.50 34:SA:284:G:OP1 38:SH:196:ARG:NH1 2.19 0.50 35:SC:191:GLU:HB2 35:SC:194:ASN:HD22 1.77 0.50 47:SX:81:VAL:O 47:SX:122:SER:OG 2.30 0.50 54:U1:18:UNK:O 54:U1:27:UNK:N 2.44 0.50 56:U3:1931:UNK:HA 56:U3:1947:UNK:HA 1.93 0.50 9:AB:74:UNK:HA 9:AB:95:UNK:HA 1.94 0.50 19:BD:286:THR:HG21 19:BD:335:GLN:HE22 1.76 0.50 19:BD:357:ASN:O 19:BD:369:ASN:ND2 2.45 0.50 34:SA:1739:C:H2’ 34:SA:1740:A:H8 1.77 0.50 Continued on next page... Page 92 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 35:SC:143:THR:HB 35:SC:205:PHE:HE2 1.77 0.50 7:5A:554:G:H1 7:5A:583:U:H3 1.59 0.50 12:AE:141:ASN:ND2 12:AE:206:TYR:OH 2.44 0.50 13:AF:201:UNK:CB 13:AF:210:UNK:O 2.60 0.50 15:B1:941:ILE:HG21 15:B1:995:ILE:H 1.76 0.50 17:BB:624:LEU:HB3 17:BB:628:HIS:H 1.77 0.50 20:BE:349:HIS:HD1 20:BE:374:SER:HG 1.46 0.50 18:BC:805:ILE:HD12 20:BE:932:VAL:HG23 1.94 0.50 25:E4:96:UNK:HA 25:E4:111:UNK:O 2.11 0.50 26:K1:152:THR:O 26:K1:155:ALA:HB3 2.11 0.50 31:R1:30:PRO:HB3 31:R1:77:ARG:HG2 1.94 0.50 34:SA:1216:C:O2’ 34:SA:1217:A:H2’ 2.12 0.50 34:SA:1615:C:N4 37:SG:78:ALA:O 2.44 0.50 41:SK:20:GLU:HB3 41:SK:23:ARG:HE 1.77 0.50 48:SY:109:ARG:NH1 48:SY:114:LYS:O 2.44 0.50 5:3F:515:SER:O 5:3F:518:LYS:NZ 2.29 0.49 8:AA:463:UNK:CB 8:AA:477:UNK:O 2.60 0.49 14:AG:405:UNK:O 14:AG:413:UNK:N 2.44 0.49 14:AG:843:UNK:O 14:AG:847:UNK:N 2.45 0.49 17:BB:622:LYS:O 17:BB:630:PHE:HA 2.12 0.49 20:BE:40:ALA:HB3 20:BE:51:VAL:HB 1.94 0.49 33:S1:239:UNK:O 33:S1:252:UNK:N 2.45 0.49 38:SH:33:GLY:HA2 38:SH:51:LYS:NZ 2.27 0.49 42:SM:77:SER:HB3 42:SM:85:VAL:HB 1.93 0.49 4:3E:358:LYS:HG2 4:3E:361:ARG:HH12 1.77 0.49 12:AE:469:LEU:O 12:AE:473:SER:CB 2.60 0.49 16:BA:171:LYS:HG2 16:BA:186:THR:HG22 1.93 0.49 16:BA:356:ASP:OD2 16:BA:830:ARG:NH2 2.45 0.49 16:BA:825:GLN:HE22 20:BE:937:THR:HG1 1.56 0.49 17:BB:229:TRP:HZ3 17:BB:236:ASP:HB2 1.77 0.49 18:BC:226:ASN:HB3 18:BC:229:LYS:HB2 1.95 0.49 18:BC:344:ARG:NH1 18:BC:345:TYR:O 2.45 0.49 22:CB:777:ASP:O 22:CB:780:GLN:NE2 2.45 0.49 27:MA:108:ARG:HA 27:MA:113:VAL:HG11 1.94 0.49 36:SF:62:LYS:HA 36:SF:65:LEU:HB2 1.94 0.49 41:SK:121:SER:HB3 41:SK:124:HIS:HB3 1.95 0.49 1:3A:63:C:H2’ 1:3A:64:A:H8 1.76 0.49 2:3B:124:SER:HA 2:3B:139:VAL:O 2.11 0.49 2:3B:299:LYS:O 2:3B:318:GLY:HA2 2.12 0.49 3:3D:21:LYS:NZ 3:3D:138:LEU:O 2.45 0.49 8:AA:37:UNK:HA 8:AA:43:UNK:HA 1.95 0.49 Continued on next page... Page 93 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 20:BE:35:ASN:ND2 20:BE:53:CYS:SG 2.80 0.49 15:B1:634:ARG:NH1 31:R1:186:PRO:O 2.46 0.49 34:SA:190:C:N4 34:SA:196:G:O6 2.45 0.49 34:SA:886:U:H2’ 34:SA:887:A:H8 1.76 0.49 47:SX:15:ASN:HD21 47:SX:72:CYS:H 1.60 0.49 12:AE:8:LEU:O 12:AE:12:ALA:HB3 2.12 0.49 19:BD:411:TYR:CZ 19:BD:423:LEU:O 2.66 0.49 22:CB:841:ASN:HA 22:CB:850:PHE:O 2.13 0.49 34:SA:283:U:C2 38:SH:188:ARG:HB2 2.47 0.49 37:SG:72:HIS:ND1 46:SR:79:TYR:OH 2.43 0.49 2:3C:204:GLY:HA2 2:3C:207:LEU:HD12 1.94 0.49 7:5A:447:G:H2’ 7:5A:448:G:C8 2.47 0.49 12:AE:1689:UNK:O 12:AE:1693:UNK:CB 2.60 0.49 16:BA:603:LEU:HB2 16:BA:612:LEU:H 1.76 0.49 16:BA:817:PHE:O 16:BA:821:MET:CB 2.59 0.49 23:E1:178:VAL:HG12 23:E1:223:GLU:HB3 1.93 0.49 28:MB:187:PRO:HB3 28:MB:220:ARG:HG3 1.95 0.49 34:SA:128:U:OP1 34:SA:264:G:O2’ 2.28 0.49 34:SA:445:A:H61 34:SA:462:G:H1’ 1.77 0.49 2:3B:205:ARG:NH2 3:3D:163:VAL:O 2.45 0.49 2:3B:88:ILE:HG12 2:3B:99:ALA:HA 1.93 0.49 5:3F:260:LEU:HD21 5:3F:283:VAL:HG11 1.94 0.49 5:3F:348:LEU:O 5:3F:356:ARG:HA 2.12 0.49 5:3F:342:ARG:HH22 6:3G:90:ALA:HA 1.77 0.49 12:AE:980:UNK:O 12:AE:984:UNK:CB 2.61 0.49 31:R1:119:LYS:HG2 31:R1:165:GLU:HG2 1.94 0.49 33:S1:371:UNK:HA 33:S1:385:UNK:HA 1.93 0.49 36:SF:182:TYR:HB2 36:SF:228:ILE:HD11 1.95 0.49 38:SH:12:SER:OG 38:SH:127:THR:O 2.31 0.49 38:SH:187:LYS:HZ1 38:SH:191:ARG:NH1 2.08 0.49 3:3D:146:ASP:HB2 4:3E:241:VAL:HG11 1.93 0.49 7:5A:62:C:H2’ 7:5A:63:G:H8 1.77 0.49 9:AB:80:UNK:N 9:AB:84:UNK:O 2.45 0.49 10:AC:599:UNK:O 10:AC:603:UNK:CB 2.61 0.49 17:BB:574:LEU:HD23 17:BB:594:ASP:HB3 1.95 0.49 22:CB:577:ARG:NH1 22:CB:624:CYS:O 2.45 0.49 30:P1:105:ARG:HH21 30:P1:163:THR:HA 1.77 0.49 31:R1:150:ALA:HB3 31:R1:169:VAL:HB 1.95 0.49 22:CB:1189:LEU:HD13 32:R2:1472:PRO:HD2 1.93 0.49 33:S1:301:UNK:N 33:S1:310:UNK:O 2.45 0.49 35:SC:129:THR:HA 35:SC:177:GLN:HA 1.94 0.49 Continued on next page... Page 94 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 42:SM:108:PRO:HB2 42:SM:135:VAL:HG22 1.93 0.49 54:U1:203:UNK:N 54:U1:207:UNK:O 2.46 0.49 28:MB:267:GLU:HG2 61:UC:9:UNK:HA 1.95 0.49 3:3D:231:ASP:HA 3:3D:237:LEU:HB2 1.95 0.49 3:3D:198:TRP:NE1 3:3D:268:MET:SD 2.78 0.49 4:3E:76:LEU:HD11 4:3E:98:ILE:HG12 1.94 0.49 5:3F:200:ASP:HB2 5:3F:211:LEU:HD11 1.94 0.49 5:3F:303:LYS:HG3 5:3F:319:TYR:HE1 1.78 0.49 7:5A:184:U:H2’ 7:5A:185:A:H8 1.76 0.49 10:AC:96:UNK:HA 10:AC:112:UNK:HA 1.95 0.49 12:AE:1490:UNK:O 12:AE:1494:UNK:CB 2.61 0.49 12:AE:917:UNK:O 12:AE:921:UNK:CB 2.61 0.49 16:BA:825:GLN:HE21 20:BE:933:ALA:HB1 1.77 0.49 22:CB:628:VAL:HA 22:CB:667:CYS:O 2.12 0.49 22:CB:814:LEU:HD12 22:CB:818:SER:HB2 1.94 0.49 23:E2:48:ALA:O 23:E2:117:SER:N 2.45 0.49 24:E3:258:VAL:O 24:E3:262:ALA:CB 2.60 0.49 33:S1:208:UNK:O 33:S1:220:UNK:N 2.45 0.49 37:SG:149:VAL:HG13 37:SG:156:ARG:HG3 1.95 0.49 5:3F:526:VAL:HG22 5:3F:539:ILE:HG12 1.94 0.49 12:AE:750:ASN:O 12:AE:754:GLU:HB2 2.12 0.49 14:AG:204:UNK:N 14:AG:208:UNK:O 2.45 0.49 15:B1:831:ARG:HE 15:B1:835:HIS:HB3 1.77 0.49 16:BA:8:SER:HB2 16:BA:703:ILE:HD11 1.94 0.49 22:CB:100:SER:O 22:CB:104:LYS:CB 2.61 0.49 23:E1:91:ILE:HG12 23:E2:132:ARG:NH1 2.28 0.49 34:SA:1213:G:O6 34:SA:1450:U:O4 2.30 0.49 35:SC:89:ASP:OD2 35:SC:99:ASN:HB2 2.13 0.49 4:3E:280:MET:HG2 4:3E:293:GLU:HA 1.94 0.49 17:BB:49:VAL:HB 17:BB:63:LEU:HB2 1.94 0.49 18:BC:331:SER:HB2 18:BC:381:VAL:HG23 1.95 0.49 20:BE:613:ASP:HB2 20:BE:620:ILE:HD13 1.95 0.49 21:CA:91:ASP:OD1 21:CA:95:LEU:N 2.45 0.49 33:S1:372:UNK:N 33:S1:384:UNK:O 2.45 0.49 36:SF:100:ARG:NH2 36:SF:121:TYR:O 2.45 0.49 40:SJ:36:THR:HG23 40:SJ:96:LEU:HB2 1.95 0.49 15:B1:143:MET:O 48:SY:144:ARG:NH1 2.46 0.49 6:3G:33:LEU:HD11 6:3G:100:ALA:HB1 1.94 0.48 12:AE:487:THR:HG21 12:AE:490:ARG:HE 1.78 0.48 19:BD:486:SER:OG 19:BD:487:GLU:N 2.45 0.48 23:E2:144:LEU:HD22 23:E2:160:LEU:HD13 1.94 0.48 Continued on next page... Page 95 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 24:E3:229:LEU:HD21 24:E3:258:VAL:HG22 1.94 0.48 34:SA:340:U:H2’ 34:SA:341:A:C8 2.48 0.48 9:AB:429:UNK:O 9:AB:433:UNK:CB 2.61 0.48 12:AE:1511:UNK:O 12:AE:1515:UNK:CB 2.61 0.48 15:B1:236:LEU:O 15:B1:239:THR:OG1 2.32 0.48 15:B1:252:GLY:O 15:B1:788:TYR:HA 2.13 0.48 20:BE:116:ALA:HB1 20:BE:133:ASP:OD1 2.13 0.48 23:E2:178:VAL:HG23 23:E2:204:VAL:HG23 1.95 0.48 34:SA:126:A:H62 34:SA:291:G:H21 0.74 0.48 47:SX:23:ARG:NH1 47:SX:66:ASN:HA 2.17 0.48 2:3C:107:VAL:HG12 2:3C:143:VAL:HA 1.94 0.48 2:3C:238:ASP:OD1 2:3C:262:LYS:NZ 2.40 0.48 4:3E:76:LEU:HD13 4:3E:101:LEU:HD22 1.95 0.48 6:3H:56:ALA:N 6:3H:81:VAL:O 2.39 0.48 15:B1:266:ARG:HB3 15:B1:815:LEU:HD12 1.95 0.48 16:BA:548:LYS:HB2 46:SR:110:THR:HG21 1.93 0.48 16:BA:584:HIS:CG 16:BA:685:GLN:HA 2.48 0.48 18:BC:231:CYS:SG 18:BC:232:LYS:N 2.87 0.48 18:BC:559:ILE:HG22 18:BC:568:MET:HB3 1.95 0.48 20:BE:588:ILE:HG12 20:BE:604:SER:HB2 1.95 0.48 20:BE:632:VAL:HG12 20:BE:643:THR:HG22 1.95 0.48 37:SG:97:LEU:O 37:SG:180:ARG:NH2 2.47 0.48 42:SM:123:VAL:HG23 42:SM:142:VAL:HA 1.95 0.48 47:SX:11:LEU:HD23 47:SX:14:ILE:HD12 1.95 0.48 58:U5:73:LEU:HD13 58:U5:101:ILE:HD12 1.95 0.48 2:3C:220:ILE:HG21 2:3C:233:LEU:HD13 1.94 0.48 7:5A:12:G:N2 7:5A:70:A:OP2 2.42 0.48 12:AE:1688:UNK:O 12:AE:1692:UNK:CB 2.61 0.48 12:AE:37:SER:O 12:AE:41:ALA:CB 2.61 0.48 12:AE:748:LEU:O 12:AE:752:ALA:HB2 2.13 0.48 16:BA:751:ILE:O 16:BA:755:TYR:CB 2.61 0.48 17:BB:851:PRO:O 17:BB:855:LYS:CB 2.61 0.48 18:BC:298:SER:OG 18:BC:299:ASN:N 2.45 0.48 31:R1:107:ALA:HB1 31:R1:170:VAL:HG11 1.94 0.48 34:SA:883:C:H2’ 34:SA:884:A:C8 2.49 0.48 49:SZ:10:ARG:O 49:SZ:24:VAL:N 2.44 0.48 54:U1:110:UNK:N 54:U1:118:UNK:O 2.46 0.48 3:3D:92:LYS:HA 3:3D:115:TYR:HB3 1.95 0.48 15:B1:66:ARG:HA 15:B1:69:VAL:HG22 1.96 0.48 15:B1:948:ILE:HG21 15:B1:951:MET:HB2 1.95 0.48 16:BA:538:GLN:HE21 16:BA:551:GLY:HA3 1.78 0.48 Continued on next page... Page 96 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 17:BB:218:ILE:HG22 17:BB:228:ILE:HG22 1.94 0.48 18:BC:127:ILE:HG23 18:BC:140:PHE:HB2 1.94 0.48 19:BD:310:GLN:HA 19:BD:326:LEU:HB2 1.95 0.48 34:SA:283:U:OP2 38:SH:188:ARG:NH1 2.42 0.48 35:SC:214:LYS:NZ 35:SC:216:LYS:HB3 2.29 0.48 40:SJ:26:LYS:NZ 40:SJ:29:LEU:HD13 2.28 0.48 41:SK:78:ARG:HH12 41:SK:91:LYS:HD3 1.78 0.48 43:SN:29:LYS:HE2 43:SN:100:TRP:HE1 1.78 0.48 56:U3:649:UNK:HA 56:U3:666:UNK:HA 1.96 0.48 5:3F:366:GLN:O 5:3F:369:LEU:HB2 2.13 0.48 7:5A:334:G:O6 7:5A:390:C:N4 2.43 0.48 8:AA:239:UNK:CB 8:AA:252:UNK:O 2.61 0.48 15:B1:610:LYS:O 31:R1:321:ARG:NH2 2.40 0.48 16:BA:499:ILE:HG22 16:BA:510:GLU:HA 1.95 0.48 22:CB:1099:VAL:HB 22:CB:1235:GLU:HB2 1.96 0.48 22:CB:855:LEU:HD22 22:CB:889:TYR:HB3 1.95 0.48 32:R2:1475:SER:N 32:R2:1512:GLU:OE2 2.45 0.48 34:SA:1512:G:H2’ 34:SA:1513:G:C8 2.49 0.48 4:3E:76:LEU:HA 4:3E:84:LYS:NZ 2.29 0.48 16:BA:784:ASP:OD1 16:BA:826:ARG:NH2 2.46 0.48 22:CB:807:LEU:HD23 22:CB:810:ILE:HD12 1.95 0.48 26:K1:140:LYS:O 26:K1:144:ARG:CB 2.61 0.48 27:MA:47:TYR:HD1 27:MA:50:ILE:HD12 1.79 0.48 31:R1:23:LEU:HB3 31:R1:332:ILE:HG13 1.95 0.48 36:SF:122:LYS:HG2 36:SF:164:LEU:HD21 1.96 0.48 57:U4:117:LEU:HG 57:U4:121:ARG:HE 1.79 0.48 61:UC:923:UNK:O 61:UC:927:UNK:N 2.46 0.48 1:3A:1:G:N1 34:SA:1124:A:C2 2.82 0.48 8:AA:385:UNK:HA 8:AA:392:UNK:HA 1.96 0.48 8:AA:466:UNK:O 8:AA:475:UNK:N 2.47 0.48 12:AE:1065:UNK:O 12:AE:1069:UNK:CB 2.62 0.48 12:AE:81:LEU:HD11 12:AE:85:VAL:HG22 1.95 0.48 14:AG:447:UNK:HA 14:AG:453:UNK:HA 1.95 0.48 18:BC:680:ASN:O 18:BC:684:LEU:CB 2.61 0.48 19:BD:539:ASP:HA 19:BD:562:LEU:HB2 1.95 0.48 34:SA:359:A:OP2 34:SA:360:A:N6 2.47 0.48 37:SG:54:LYS:NZ 37:SG:131:GLN:HE22 2.12 0.48 38:SH:77:LEU:HD13 38:SH:84:TYR:HB2 1.95 0.48 46:SR:5:PRO:HG2 46:SR:24:ALA:HB2 1.96 0.48 2:3C:208:ILE:HD12 4:3E:156:ALA:HA 1.95 0.48 4:3E:397:ARG:HE 4:3E:401:LEU:HG 1.79 0.48 Continued on next page... Page 97 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 5:3F:402:ILE:HA 5:3F:417:SER:HA 1.96 0.48 16:BA:81:LEU:HB2 16:BA:90:LEU:HB2 1.96 0.48 20:BE:97:LYS:HD3 20:BE:109:LEU:HD21 1.96 0.48 20:BE:132:THR:OG1 20:BE:136:SER:O 2.32 0.48 20:BE:567:ILE:HB 20:BE:581:LEU:HB2 1.95 0.48 16:BA:825:GLN:NE2 20:BE:937:THR:OG1 2.37 0.48 22:CB:551:GLU:O 22:CB:555:PHE:HB2 2.13 0.48 4:3E:363:LEU:O 4:3E:367:ALA:HB2 2.13 0.48 12:AE:817:UNK:O 12:AE:821:UNK:CB 2.62 0.48 15:B1:145:ARG:NE 15:B1:210:LYS:O 2.39 0.48 16:BA:446:CYS:HA 16:BA:456:HIS:O 2.14 0.48 18:BC:38:ASP:OD2 18:BC:309:GLN:NE2 2.47 0.48 21:CA:183:ASP:O 21:CA:187:HIS:CB 2.62 0.48 22:CB:1100:VAL:O 22:CB:1181:VAL:HA 2.13 0.48 22:CB:656:ARG:HH11 22:CB:658:PHE:HD1 1.62 0.48 22:CB:761:ARG:HB3 22:CB:896:THR:HG23 1.95 0.48 17:BB:603:ASP:HA 31:R1:200:VAL:HG12 1.96 0.48 46:SR:110:THR:HA 46:SR:113:ASP:HB2 1.95 0.48 5:3F:152:VAL:HG22 5:3F:562:GLY:HA2 1.96 0.47 5:3F:134:ARG:HB2 5:3F:484:SER:HA 1.94 0.47 5:3F:541:ALA:O 5:3F:563:ILE:HA 2.14 0.47 7:5A:98:G:O6 7:5A:146:G:O6 2.31 0.47 12:AE:608:ASN:HD21 12:AE:662:PRO:HG3 1.79 0.47 15:B1:548:ASN:HD22 15:B1:551:LYS:HE3 1.78 0.47 16:BA:355:PRO:O 16:BA:822:ARG:NH1 2.47 0.47 19:BD:296:GLN:HG3 19:BD:353:ARG:HH12 1.78 0.47 21:CA:94:GLY:HA3 21:CA:121:ARG:HH12 1.79 0.47 34:SA:539:G:H8 34:SA:539:G:OP2 1.97 0.47 35:SC:139:ALA:HA 35:SC:212:VAL:HA 1.97 0.47 1:3A:260:U:O4 3:3D:377:ARG:NH2 2.46 0.47 7:5A:184:U:H2’ 7:5A:185:A:C8 2.49 0.47 12:AE:347:PHE:O 12:AE:351:TYR:CB 2.62 0.47 12:AE:600:LEU:HD22 12:AE:611:ILE:HG22 1.96 0.47 14:AG:244:UNK:HA 14:AG:250:UNK:HA 1.96 0.47 15:B1:977:LYS:H 15:B1:989:ALA:HA 1.80 0.47 16:BA:57:ASN:OD1 16:BA:619:ARG:NH2 2.47 0.47 20:BE:583:GLY:C 20:BE:610:ARG:HH12 2.16 0.47 22:CB:545:ALA:HB2 22:CB:565:LYS:HE2 1.96 0.47 22:CB:589:LYS:O 22:CB:609:ASN:ND2 2.47 0.47 23:E2:115:GLN:HE21 23:E2:119:GLY:HA2 1.79 0.47 33:S1:167:UNK:N 33:S1:179:UNK:O 2.47 0.47 Continued on next page... Page 98 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:1217:A:H2 34:SA:1448:G:N2 2.10 0.47 34:SA:1474:G:H2’ 34:SA:1475:A:H8 1.78 0.47 34:SA:283:U:O4’ 34:SA:283:U:C6 2.67 0.47 34:SA:871:G:H2’ 34:SA:872:G:C8 2.49 0.47 35:SC:146:GLN:HB2 35:SC:149:GLN:HE21 1.78 0.47 37:SG:76:ARG:O 37:SG:83:ARG:NE 2.40 0.47 39:SI:14:THR:OG1 39:SI:15:GLU:N 2.46 0.47 44:SO:67:THR:HG23 44:SO:69:ASN:H 1.79 0.47 1:3A:80:U:OP2 6:3H:95:ARG:HD2 2.14 0.47 2:3B:233:LEU:O 3:3D:151:GLN:NE2 2.47 0.47 12:AE:261:LEU:O 12:AE:265:ALA:CB 2.62 0.47 12:AE:288:ALA:HB1 12:AE:292:LYS:HG2 1.96 0.47 12:AE:728:GLU:HG3 12:AE:730:GLN:H 1.79 0.47 13:AF:39:UNK:N 13:AF:43:UNK:O 2.47 0.47 17:BB:20:ASN:HD21 17:BB:23:CYS:H 1.61 0.47 18:BC:487:ARG:HH11 18:BC:523:GLY:HA2 1.79 0.47 24:E3:411:ARG:O 24:E3:447:ARG:NH2 2.48 0.47 36:SF:19:LEU:HD21 36:SF:108:ARG:HH21 1.79 0.47 38:SH:63:MET:SD 38:SH:98:ARG:NH2 2.87 0.47 40:SJ:64:ASN:HA 40:SJ:75:LYS:HA 1.96 0.47 5:3F:189:THR:HG21 5:3F:563:ILE:HD11 1.97 0.47 6:3G:77:PRO:HG2 6:3G:118:LYS:HE3 1.96 0.47 12:AE:134:MET:HA 12:AE:137:LEU:HD12 1.97 0.47 12:AE:196:LEU:HD11 12:AE:213:THR:HG21 1.95 0.47 17:BB:261:PHE:O 17:BB:272:PHE:HA 2.15 0.47 20:BE:865:ILE:HA 20:BE:868:LEU:HD12 1.95 0.47 22:CB:508:SER:HA 22:CB:512:LEU:HB2 1.96 0.47 24:E3:301:TYR:O 24:E3:304:TYR:HB3 2.13 0.47 32:R2:1629:PHE:HB2 32:R2:1661:GLN:HE21 1.79 0.47 22:CB:173:ASN:ND2 34:SA:1686:C:OP1 2.46 0.47 34:SA:549:G:H2’ 34:SA:550:A:H8 1.78 0.47 34:SA:629:U:H2’ 34:SA:630:A:H8 1.80 0.47 34:SA:899:G:H2’ 34:SA:900:A:H8 1.79 0.47 40:SJ:44:HIS:O 40:SJ:56:ARG:N 2.47 0.47 41:SK:17:ARG:O 41:SK:23:ARG:NH2 2.47 0.47 48:SY:127:VAL:HG13 48:SY:132:LEU:HD21 1.96 0.47 58:U5:87:LEU:HA 58:U5:88:THR:HA 1.66 0.47 1:3A:246:A:OP2 5:3F:207:ARG:NH2 2.48 0.47 3:3D:176:GLN:O 3:3D:180:LEU:CB 2.62 0.47 3:3D:221:LEU:HD13 3:3D:275:ALA:HB3 1.95 0.47 8:AA:152:UNK:CB 8:AA:156:UNK:O 2.62 0.47 Continued on next page... Page 99 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 8:AA:273:UNK:CB 8:AA:281:UNK:O 2.62 0.47 8:AA:302:UNK:O 8:AA:311:UNK:N 2.47 0.47 8:AA:384:UNK:O 8:AA:393:UNK:N 2.47 0.47 12:AE:1148:UNK:O 12:AE:1152:UNK:CB 2.62 0.47 12:AE:261:LEU:O 12:AE:265:ALA:HB2 2.14 0.47 18:BC:304:LEU:HD22 18:BC:311:LEU:HD11 1.97 0.47 18:BC:465:LYS:HE3 18:BC:520:LEU:HD11 1.95 0.47 22:CB:1159:ASN:HD22 22:CB:1201:ASP:HB3 1.79 0.47 22:CB:235:LEU:O 22:CB:239:LEU:HB3 2.15 0.47 25:E4:141:UNK:O 25:E4:150:UNK:N 2.46 0.47 34:SA:418:G:O2’ 38:SH:72:ARG:NH1 2.48 0.47 41:SK:57:ARG:NH1 57:U4:88:ASP:O 2.47 0.47 2:3C:240:VAL:HG23 2:3C:261:LEU:HD13 1.95 0.47 5:3F:553:ILE:HD13 5:3F:556:VAL:HG12 1.95 0.47 7:5A:145:A:H2’ 7:5A:146:G:C8 2.50 0.47 14:AG:823:UNK:O 14:AG:827:UNK:CB 2.63 0.47 18:BC:585:ASN:O 18:BC:587:GLN:NE2 2.47 0.47 18:BC:15:ILE:N 18:BC:641:PHE:O 2.47 0.47 22:CB:715:SER:O 22:CB:719:THR:OG1 2.30 0.47 23:E1:48:ALA:HB3 23:E1:116:THR:HA 1.97 0.47 24:E3:268:SER:HB2 34:SA:1233:G:H5’ 1.97 0.47 30:P1:158:PHE:HE1 30:P1:174:LEU:HB2 1.80 0.47 37:SG:113:ILE:HG23 37:SG:191:ALA:HB2 1.97 0.47 42:SM:33:ARG:NH1 42:SM:61:THR:HG21 2.29 0.47 46:SR:21:HIS:CE1 46:SR:68:ARG:HH12 2.32 0.47 1:3A:47:G:H2’ 1:3A:48:A:H8 1.79 0.47 9:AB:59:UNK:O 9:AB:68:UNK:N 2.48 0.47 11:AD:461:UNK:O 11:AD:465:UNK:CB 2.62 0.47 12:AE:506:TYR:O 12:AE:510:SER:HB2 2.14 0.47 18:BC:362:LEU:HB2 18:BC:384:TYR:HB2 1.97 0.47 22:CB:826:SER:O 22:CB:838:VAL:HA 2.14 0.47 25:E4:157:UNK:HA 25:E4:171:UNK:O 2.14 0.47 32:R2:1505:ILE:HB 32:R2:1513:LYS:HD3 1.96 0.47 32:R2:1502:LEU:HA 32:R2:1513:LYS:HE3 1.96 0.47 34:SA:1226:A:H3’ 43:SN:116:VAL:HG11 1.96 0.47 34:SA:417:A:OP1 38:SH:59:GLN:NE2 2.48 0.47 36:SF:139:VAL:HG13 36:SF:150:PRO:HG3 1.97 0.47 41:SK:171:ARG:HH21 41:SK:175:ARG:HH22 1.63 0.47 49:SZ:83:LYS:HD2 49:SZ:84:LYS:NZ 2.29 0.47 4:3E:76:LEU:HD23 4:3E:84:LYS:HZ1 1.80 0.47 5:3F:316:GLU:OE2 5:3F:351:ILE:HG23 2.15 0.47 Continued on next page... Page 100 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 6:3G:56:ALA:HB3 6:3G:82:PRO:HA 1.96 0.47 8:AA:507:UNK:O 8:AA:516:UNK:CB 2.62 0.47 19:BD:454:GLY:N 19:BD:487:GLU:OE1 2.40 0.47 21:CA:13:PHE:HA 21:CA:39:ALA:O 2.15 0.47 22:CB:924:LEU:HD21 22:CB:1181:VAL:HG13 1.95 0.47 22:CB:207:LEU:HD11 22:CB:294:LEU:HD22 1.97 0.47 1:3A:1:G:C6 34:SA:1124:A:C2 3.02 0.47 34:SA:1584:G:N2 34:SA:1585:U:O4 2.37 0.47 34:SA:1718:G:H5’ 34:SA:1719:A:OP2 2.15 0.47 34:SA:179:A:H4’ 34:SA:180:A:C4 2.49 0.47 39:SI:137:GLY:HA3 39:SI:153:LEU:HD12 1.96 0.47 39:SI:139:ARG:HB3 47:SX:51:GLU:OE2 2.14 0.47 2:3C:96:VAL:HG11 2:3C:155:ILE:HG21 1.97 0.47 3:3D:199:TYR:HE2 3:3D:206:LEU:HB3 1.80 0.47 4:3E:191:HIS:O 4:3E:215:ARG:NH2 2.48 0.47 2:3C:231:ARG:NH1 4:3E:7:GLU:O 2.41 0.47 5:3F:195:GLN:HE21 5:3F:213:TYR:HB2 1.79 0.47 8:AA:319:UNK:CB 8:AA:332:UNK:O 2.63 0.47 16:BA:155:SER:HB2 16:BA:160:PHE:HB3 1.97 0.47 18:BC:345:TYR:HD2 18:BC:350:LEU:HD12 1.80 0.47 20:BE:275:PHE:HE2 20:BE:287:LEU:HG 1.80 0.47 20:BE:551:TYR:OH 20:BE:556:ASP:OD1 2.30 0.47 22:CB:207:LEU:HB2 22:CB:295:LEU:O 2.14 0.47 34:SA:870:C:H2’ 34:SA:871:G:H8 1.79 0.47 1:3A:329:C:H2’ 1:3A:330:A:C8 2.50 0.47 2:3B:169:LYS:O 2:3B:238:ASP:N 2.47 0.47 7:5A:226:U:O2’ 7:5A:228:A:N7 2.48 0.47 12:AE:8:LEU:O 12:AE:12:ALA:CB 2.63 0.47 16:BA:50:PHE:H 16:BA:86:ALA:HB2 1.80 0.47 16:BA:605:ASP:HB3 16:BA:611:LEU:HD21 1.96 0.47 17:BB:621:VAL:HA 17:BB:631:PHE:O 2.14 0.47 33:S1:118:UNK:N 33:S1:127:UNK:O 2.48 0.47 61:UC:702:UNK:O 61:UC:706:UNK:N 2.48 0.47 12:AE:1439:UNK:O 12:AE:1443:UNK:CB 2.63 0.47 17:BB:125:THR:H 17:BB:140:SER:HA 1.80 0.47 17:BB:545:TYR:HA 17:BB:559:PHE:HA 1.97 0.47 18:BC:455:LEU:HD13 18:BC:465:LYS:HD3 1.96 0.47 20:BE:511:ASP:OD2 20:BE:515:ARG:HB2 2.15 0.47 21:CA:169:VAL:HB 22:CB:1140:SER:HB2 1.95 0.47 24:E3:332:VAL:H 34:SA:1435:G:H5’ 1.80 0.47 34:SA:1463:C:H2’ 34:SA:1464:G:C8 2.50 0.47 Continued on next page... Page 101 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 37:SG:103:ASN:HA 37:SG:106:LYS:HD2 1.97 0.47 38:SH:188:ARG:HA 38:SH:188:ARG:HD3 1.40 0.47 47:SX:72:CYS:HB3 47:SX:129:VAL:HG23 1.96 0.47 54:U1:272:UNK:N 54:U1:284:UNK:O 2.48 0.47 41:SK:53:ARG:HH12 57:U4:92:ALA:HB2 1.80 0.47 2:3B:110:ASN:HB3 2:3B:140:GLU:HB2 1.98 0.46 6:3H:111:LYS:HA 6:3H:114:ILE:HD12 1.96 0.46 7:5A:340:U:H2’ 7:5A:341:G:H8 1.81 0.46 7:5A:578:G:H2’ 7:5A:579:G:H8 1.80 0.46 8:AA:405:UNK:N 8:AA:409:UNK:O 2.47 0.46 12:AE:109:TRP:HH2 12:AE:118:THR:HG21 1.80 0.46 12:AE:1742:UNK:O 12:AE:1746:UNK:CB 2.63 0.46 12:AE:738:LEU:HD22 12:AE:774:ASN:HB3 1.96 0.46 16:BA:193:TYR:HB3 16:BA:211:LYS:HD3 1.97 0.46 20:BE:633:LYS:HB2 20:BE:642:ALA:HB3 1.95 0.46 20:BE:53:CYS:HB2 20:BE:83:LEU:HD11 1.96 0.46 21:CA:181:LYS:HZ3 22:CB:808:LEU:HB3 1.80 0.46 24:E3:335:ALA:O 24:E3:339:GLY:HA3 2.15 0.46 34:SA:1588:G:N2 34:SA:1608:U:O2 2.31 0.46 34:SA:884:A:H2’ 34:SA:885:G:C8 2.50 0.46 35:SC:34:ALA:HB3 35:SC:41:ARG:HA 1.96 0.46 39:SI:76:LYS:HA 39:SI:79:ARG:NH1 2.30 0.46 40:SJ:48:THR:OG1 40:SJ:52:ASN:O 2.28 0.46 43:SN:131:ASP:HA 43:SN:134:SER:HB2 1.98 0.46 48:SY:75:GLN:HA 48:SY:82:LYS:HA 1.97 0.46 10:AC:411:UNK:O 10:AC:415:UNK:CB 2.63 0.46 10:AC:613:UNK:O 10:AC:617:UNK:CB 2.63 0.46 12:AE:120:TRP:O 12:AE:124:ARG:CB 2.63 0.46 20:BE:257:ARG:HD3 20:BE:263:HIS:HD2 1.79 0.46 23:E2:164:LYS:NZ 34:SA:1575:G:OP1 2.48 0.46 34:SA:356:G:H2’ 34:SA:357:G:H8 1.80 0.46 35:SC:70:LEU:HA 35:SC:73:LEU:HB2 1.96 0.46 36:SF:79:ASP:HB3 36:SF:82:TYR:HB2 1.97 0.46 58:U5:242:ILE:HB 58:U5:254:ILE:HB 1.97 0.46 3:3D:373:GLY:O 3:3D:377:ARG:HB2 2.15 0.46 12:AE:1382:UNK:O 12:AE:1386:UNK:CB 2.64 0.46 17:BB:149:ASP:HB3 17:BB:153:GLU:H 1.80 0.46 17:BB:437:LYS:HG2 17:BB:481:LEU:HD13 1.97 0.46 19:BD:275:TYR:HE2 19:BD:282:ASN:HD22 1.62 0.46 22:CB:419:TYR:O 22:CB:423:LYS:HB2 2.14 0.46 24:E3:206:LEU:N 24:E3:207:PRO:HD2 2.17 0.46 Continued on next page... Page 102 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 29:MC:54:UNK:O 29:MC:58:UNK:N 2.49 0.46 30:P1:103:PRO:HB3 30:P1:135:LYS:HE2 1.96 0.46 33:S1:210:UNK:N 33:S1:218:UNK:O 2.48 0.46 39:SI:48:GLU:OE2 39:SI:88:ARG:NE 2.48 0.46 45:SP:29:HIS:HB3 45:SP:41:ARG:HG3 1.96 0.46 54:U1:158:UNK:N 54:U1:171:UNK:O 2.49 0.46 3:3D:291:TYR:OH 4:3E:251:ASP:OD1 2.12 0.46 7:5A:349:G:OP2 7:5A:349:G:H8 1.98 0.46 8:AA:219:UNK:N 8:AA:233:UNK:O 2.48 0.46 12:AE:227:ASN:HD22 12:AE:230:LYS:HD2 1.81 0.46 16:BA:307:THR:O 16:BA:320:GLY:N 2.47 0.46 17:BB:351:LEU:HA 17:BB:372:ARG:HG2 1.97 0.46 23:E1:103:PRO:O 23:E1:107:ALA:N 2.44 0.46 23:E1:91:ILE:HG12 23:E2:132:ARG:HH12 1.81 0.46 12:AE:1743:UNK:O 12:AE:1747:UNK:CB 2.63 0.46 12:AE:736:PHE:O 12:AE:740:ALA:HB2 2.16 0.46 15:B1:895:PRO:HA 15:B1:918:ILE:HG13 1.98 0.46 17:BB:88:HIS:CD2 17:BB:90:ASP:H 2.33 0.46 23:E1:149:SER:HB2 23:E1:158:LYS:HG2 1.98 0.46 26:K1:100:LEU:O 26:K1:103:LEU:HB2 2.16 0.46 34:SA:384:G:H5” 40:SJ:22:ARG:HB3 1.97 0.46 35:SC:39:GLU:N 35:SC:74:GLN:OE1 2.48 0.46 37:SG:200:ASN:HB2 37:SG:208:SER:HB3 1.97 0.46 43:SN:40:GLY:O 43:SN:124:LYS:N 2.40 0.46 1:3A:254:A:H5’ 1:3A:255:U:H3’ 1.97 0.46 2:3B:291:GLN:OE1 2:3B:294:ARG:NH2 2.45 0.46 7:5A:447:G:H2’ 7:5A:448:G:H8 1.79 0.46 12:AE:502:ILE:HA 12:AE:508:ALA:HB2 1.96 0.46 15:B1:824:ILE:HA 15:B1:886:TYR:HA 1.96 0.46 17:BB:20:ASN:ND2 17:BB:23:CYS:H 2.14 0.46 17:BB:368:PRO:HA 17:BB:374:PRO:HA 1.97 0.46 19:BD:305:SER:OG 19:BD:306:ASN:N 2.49 0.46 22:CB:1059:PRO:O 22:CB:1063:ARG:CB 2.63 0.46 23:E2:31:LEU:HB3 23:E2:111:GLN:HE21 1.79 0.46 24:E3:269:ASN:ND2 34:SA:1233:G:O2’ 2.33 0.46 34:SA:1483:A:H2’ 34:SA:1484:G:C8 2.50 0.46 34:SA:294:C:H2’ 34:SA:295:A:C8 2.50 0.46 38:SH:88:ARG:HB3 38:SH:91:GLU:HB2 1.98 0.46 41:SK:70:LEU:O 41:SK:74:ASN:ND2 2.49 0.46 48:SY:103:LEU:N 48:SY:126:LYS:O 2.47 0.46 58:U5:75:ILE:HG12 58:U5:101:ILE:HB 1.97 0.46 Continued on next page... Page 103 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 3:3D:19:LYS:HE3 3:3D:142:LEU:HD13 1.97 0.46 5:3F:405:VAL:HG23 5:3F:415:THR:HG22 1.98 0.46 9:AB:442:UNK:O 9:AB:446:UNK:CB 2.64 0.46 16:BA:597:ASN:ND2 16:BA:681:VAL:O 2.48 0.46 16:BA:8:SER:H 16:BA:703:ILE:HD11 1.79 0.46 17:BB:103:ILE:HB 17:BB:117:PHE:HB2 1.97 0.46 20:BE:578:VAL:HB 20:BE:579:ARG:HH11 1.81 0.46 20:BE:82:ALA:H 20:BE:93:ALA:HB3 1.81 0.46 22:CB:805:ALA:HA 22:CB:808:LEU:HD12 1.97 0.46 34:SA:153:G:H1 34:SA:161:U:H3 1.63 0.46 40:SJ:105:ASP:OD2 40:SJ:108:PRO:HD3 2.15 0.46 40:SJ:11:ARG:NH1 40:SJ:17:LYS:HB2 2.31 0.46 46:SR:29:ILE:HA 46:SR:65:ILE:HB 1.98 0.46 49:SZ:8:ARG:HB2 49:SZ:26:ASP:HB3 1.96 0.46 2:3B:268:VAL:HG22 2:3B:317:VAL:HG22 1.98 0.46 5:3F:192:LYS:NZ 5:3F:550:GLY:HA2 2.31 0.46 9:AB:276:UNK:CB 9:AB:280:UNK:O 2.64 0.46 12:AE:1069:UNK:O 12:AE:1073:UNK:CB 2.64 0.46 16:BA:495:LYS:N 16:BA:517:ASP:OD1 2.48 0.46 19:BD:436:ASP:HB2 19:BD:444:ILE:HD11 1.98 0.46 19:BD:521:LEU:HA 19:BD:531:CYS:O 2.15 0.46 33:S1:241:UNK:O 33:S1:250:UNK:N 2.49 0.46 34:SA:1648:A:H2’ 34:SA:1649:G:C8 2.51 0.46 41:SK:67:PRO:HA 41:SK:70:LEU:HB2 1.98 0.46 41:SK:84:GLY:HA2 41:SK:150:LEU:HD22 1.98 0.46 7:5A:250:G:O6 7:5A:262:U:O4 2.34 0.46 7:5A:291:G:H2’ 7:5A:292:A:C8 2.51 0.46 7:5A:334:G:H1 7:5A:389:U:H3 1.62 0.46 12:AE:347:PHE:O 12:AE:351:TYR:HB2 2.16 0.46 14:AG:302:UNK:O 14:AG:315:UNK:CB 2.64 0.46 16:BA:75:GLU:HA 16:BA:98:LYS:HB3 1.98 0.46 18:BC:269:LEU:O 18:BC:278:LEU:N 2.41 0.46 20:BE:143:SER:OG 20:BE:147:ASP:OD2 2.30 0.46 22:CB:96:GLU:O 22:CB:100:SER:HB2 2.16 0.46 26:K1:126:ILE:HB 26:K1:171:VAL:HB 1.98 0.46 33:S1:249:UNK:N 33:S1:261:UNK:O 2.49 0.46 34:SA:1268:G:H2’ 34:SA:1270:G:H8 1.80 0.46 34:SA:1474:G:H2’ 34:SA:1475:A:C8 2.51 0.46 34:SA:478:A:H62 34:SA:539:G:H22 1.64 0.46 40:SJ:36:THR:OG1 40:SJ:59:ARG:N 2.44 0.46 1:3A:113:G:H1’ 1:3A:257:A:H61 1.80 0.46 Continued on next page... Page 104 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 1:3A:46:U:H2’ 1:3A:47:G:H8 1.80 0.46 5:3F:160:ILE:HB 5:3F:542:SER:HB3 1.98 0.46 7:5A:271:G:H2’ 7:5A:272:A:H8 1.81 0.46 7:5A:511:G:O6 7:5A:525:U:O4 2.33 0.46 8:AA:355:UNK:CB 8:AA:359:UNK:O 2.64 0.46 16:BA:300:MET:N 16:BA:300:MET:SD 2.89 0.46 19:BD:372:HIS:HA 19:BD:407:PHE:H 1.81 0.46 21:CA:146:ASN:HB3 21:CA:150:LYS:HZ2 1.81 0.46 21:CA:175:LEU:HD21 22:CB:827:ARG:HG2 1.97 0.46 22:CB:179:LYS:HB2 22:CB:210:PRO:HG2 1.97 0.46 34:SA:870:C:H2’ 34:SA:871:G:C8 2.51 0.46 10:AC:646:UNK:O 10:AC:650:UNK:CB 2.65 0.45 13:AF:71:UNK:CB 13:AF:75:UNK:O 2.64 0.45 16:BA:150:THR:H 16:BA:165:SER:HA 1.80 0.45 16:BA:368:LYS:HG2 16:BA:384:GLU:HG2 1.97 0.45 22:CB:142:GLU:HB2 22:CB:144:LYS:HE2 1.97 0.45 22:CB:218:ASP:OD2 22:CB:227:LYS:NZ 2.50 0.45 22:CB:671:THR:OG1 22:CB:718:LYS:O 2.34 0.45 22:CB:738:TYR:O 22:CB:742:PHE:HB2 2.17 0.45 33:S1:137:UNK:N 33:S1:146:UNK:O 2.48 0.45 34:SA:1588:G:O6 34:SA:1608:U:O4 2.34 0.45 34:SA:429:G:H2’ 34:SA:430:G:H8 1.81 0.45 47:SX:12:ASN:O 47:SX:16:ASN:ND2 2.49 0.45 49:SZ:7:ILE:HG21 49:SZ:44:LEU:HD21 1.98 0.45 5:3F:188:TYR:HB3 5:3F:196:LEU:HD11 1.97 0.45 15:B1:623:LYS:HA 15:B1:626:LYS:HE2 1.97 0.45 17:BB:749:GLY:HA2 17:BB:752:LEU:HD12 1.98 0.45 22:CB:975:LEU:HD23 22:CB:1041:VAL:HG22 1.97 0.45 36:SF:75:LYS:HZ1 36:SF:77:ARG:HH12 1.57 0.45 1:3A:46:U:H2’ 1:3A:47:G:C8 2.51 0.45 12:AE:1057:UNK:HA 12:AE:1065:UNK:HA 1.99 0.45 12:AE:163:CYS:HA 12:AE:164:LEU:HA 1.70 0.45 13:AF:474:UNK:O 13:AF:478:UNK:CB 2.65 0.45 15:B1:965:GLN:HA 15:B1:975:GLU:HG2 1.98 0.45 18:BC:220:ILE:HB 18:BC:237:LEU:HB3 1.99 0.45 20:BE:430:ILE:O 20:BE:442:THR:HA 2.16 0.45 31:R1:155:LYS:HG2 31:R1:234:ASN:HA 1.97 0.45 34:SA:1082:C:OP2 34:SA:1083:G:OP2 2.35 0.45 34:SA:215:A:OP2 34:SA:215:A:H3’ 2.17 0.45 22:CB:897:ARG:HE 35:SC:179:SER:HB2 1.81 0.45 39:SI:143:LEU:HD22 39:SI:147:ASN:HD22 1.81 0.45 Continued on next page... Page 105 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 44:SO:101:HIS:HA 44:SO:104:ARG:HE 1.82 0.45 54:U1:151:UNK:N 54:U1:159:UNK:O 2.50 0.45 2:3B:107:VAL:HG12 2:3B:143:VAL:HA 1.99 0.45 2:3B:299:LYS:HB2 2:3B:319:ARG:HB2 1.97 0.45 4:3E:373:TYR:HB3 4:3E:379:ASP:OD2 2.16 0.45 17:BB:59:LEU:H 17:BB:383:HIS:HB3 1.79 0.45 20:BE:383:ASP:OD1 20:BE:384:ALA:N 2.49 0.45 20:BE:474:PHE:HA 20:BE:487:TYR:O 2.17 0.45 23:E2:28:PRO:HA 23:E2:29:PRO:HD3 1.88 0.45 24:E3:449:HIS:CD2 24:E3:451:ASP:H 2.34 0.45 25:E4:112:UNK:CB 25:E4:116:UNK:O 2.65 0.45 34:SA:1118:G:H2’ 34:SA:1119:G:C8 2.51 0.45 34:SA:1647:U:H2’ 34:SA:1648:A:C8 2.52 0.45 34:SA:93:A:H61 34:SA:396:G:H1’ 1.80 0.45 38:SH:57:ASP:HB3 38:SH:106:LEU:HD23 1.98 0.45 40:SJ:81:VAL:HA 40:SJ:102:VAL:HG12 1.97 0.45 34:SA:332:U:H5’ 40:SJ:30:GLY:HA2 1.98 0.45 16:BA:514:VAL:HA 46:SR:118:ILE:HD13 1.98 0.45 4:3E:296:GLY:HA2 4:3E:299:LEU:HD12 1.99 0.45 7:5A:529:A:H2’ 7:5A:530:A:C8 2.52 0.45 8:AA:283:UNK:HA 8:AA:289:UNK:HA 1.98 0.45 12:AE:29:HIS:CD2 12:AE:123:ARG:HH21 2.35 0.45 14:AG:394:UNK:O 14:AG:406:UNK:CB 2.64 0.45 16:BA:263:VAL:HG22 16:BA:279:PHE:HE1 1.82 0.45 17:BB:226:VAL:O 17:BB:241:LYS:HA 2.17 0.45 17:BB:386:GLU:HB3 17:BB:390:GLN:HE22 1.80 0.45 17:BB:617:SER:O 17:BB:634:SER:OG 2.29 0.45 18:BC:377:LEU:HD12 18:BC:381:VAL:HG12 1.99 0.45 22:CB:1059:PRO:O 22:CB:1063:ARG:HB2 2.16 0.45 22:CB:345:TYR:HH 22:CB:472:THR:HG1 1.60 0.45 22:CB:949:PHE:O 22:CB:960:LYS:NZ 2.49 0.45 28:MB:169:ASN:ND2 28:MB:253:ARG:O 2.49 0.45 31:R1:281:GLU:OE2 31:R1:285:LYS:HE2 2.17 0.45 34:SA:391:A:H2’ 34:SA:392:G:C8 2.51 0.45 34:SA:913:G:H4’ 34:SA:914:G:OP2 2.15 0.45 38:SH:70:PRO:HD3 38:SH:101:ILE:HD12 1.98 0.45 1:3A:330:A:H2’ 1:3A:331:A:H8 1.81 0.45 1:3A:67:G:H2’ 1:3A:68:A:H8 1.81 0.45 7:5A:589:U:H2’ 7:5A:590:G:H8 1.82 0.45 11:AD:509:UNK:O 11:AD:513:UNK:CB 2.64 0.45 17:BB:748:ALA:HA 17:BB:751:ARG:HE 1.82 0.45 Continued on next page... Page 106 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 18:BC:751:LYS:H 18:BC:753:HIS:CE1 2.35 0.45 20:BE:541:LYS:NZ 20:BE:542:LEU:O 2.48 0.45 28:MB:205:ILE:O 28:MB:209:LEU:N 2.49 0.45 33:S1:219:UNK:O 33:S1:227:UNK:N 2.50 0.45 34:SA:1584:G:H1’ 34:SA:1585:U:H5 1.82 0.45 34:SA:15:U:H2’ 34:SA:16:G:C8 2.52 0.45 34:SA:406:U:H2’ 34:SA:407:A:C8 2.52 0.45 58:U5:52:ASN:HD21 58:U5:228:LYS:HE3 1.82 0.45 1:3A:3:C:H2’ 1:3A:4:G:C8 2.51 0.45 4:3E:225:GLU:HG3 4:3E:226:ILE:HG13 1.99 0.45 8:AA:116:UNK:O 8:AA:129:UNK:CB 2.65 0.45 12:AE:121:LEU:HA 12:AE:125:PHE:HD2 1.82 0.45 15:B1:201:ASN:OD1 15:B1:204:ARG:NH2 2.49 0.45 16:BA:16:ARG:HB2 16:BA:34:GLY:H 1.82 0.45 16:BA:60:ALA:H 16:BA:73:ILE:H 1.64 0.45 19:BD:273:ARG:HA 19:BD:287:SER:HA 1.99 0.45 21:CA:53:LEU:HD12 21:CA:126:LEU:HD23 1.99 0.45 22:CB:167:PRO:HD3 22:CB:226:HIS:CE1 2.51 0.45 22:CB:419:TYR:HE1 22:CB:499:LEU:HD22 1.82 0.45 34:SA:445:A:C4 34:SA:525:A:H4’ 2.52 0.45 57:U4:81:ASP:OD2 57:U4:84:ARG:NE 2.36 0.45 3:3D:74:VAL:HB 3:3D:78:LEU:HD22 1.99 0.45 6:3G:111:LYS:HA 6:3G:114:ILE:HD12 1.98 0.45 7:5A:357:G:H2’ 7:5A:358:G:H8 1.82 0.45 7:5A:71:U:H2’ 7:5A:72:G:C8 2.52 0.45 12:AE:1520:UNK:O 12:AE:1524:UNK:CB 2.65 0.45 12:AE:25:ARG:O 12:AE:29:HIS:CB 2.64 0.45 15:B1:190:ILE:HG13 15:B1:190:ILE:H 1.60 0.45 17:BB:212:VAL:HB 17:BB:217:LEU:HD23 1.98 0.45 17:BB:530:LEU:HD23 17:BB:551:LEU:HG 1.99 0.45 18:BC:43:ILE:HB 18:BC:56:ILE:O 2.17 0.45 18:BC:680:ASN:O 18:BC:684:LEU:HB3 2.17 0.45 22:CB:268:LEU:HB3 22:CB:294:LEU:HB2 1.98 0.45 22:CB:232:LEU:HD13 22:CB:296:ILE:HD11 1.98 0.45 24:E3:388:ASP:OD1 24:E3:436:GLN:NE2 2.48 0.45 35:SC:118:GLN:OE1 35:SC:208:GLN:NE2 2.49 0.45 37:SG:80:LYS:HB3 37:SG:83:ARG:HB2 1.98 0.45 38:SH:102:VAL:HG13 38:SH:106:LEU:HD12 1.98 0.45 47:SX:12:ASN:OD1 47:SX:16:ASN:ND2 2.49 0.45 54:U1:265:UNK:HA 54:U1:272:UNK:HA 1.99 0.45 58:U5:70:LEU:HB3 58:U5:123:ASP:HB2 1.98 0.45 Continued on next page... Page 107 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 10:AC:4:UNK:HA 10:AC:284:UNK:HA 1.99 0.45 10:AC:530:UNK:O 10:AC:534:UNK:CB 2.65 0.45 12:AE:1095:UNK:O 12:AE:1099:UNK:CB 2.65 0.45 13:AF:3:UNK:O 13:AF:285:UNK:N 2.50 0.45 17:BB:426:ARG:NH1 17:BB:458:ASP:O 2.49 0.45 17:BB:555:VAL:HG22 17:BB:569:LEU:HB2 1.97 0.45 22:CB:1147:TYR:O 22:CB:1151:LYS:HB2 2.17 0.45 22:CB:879:GLU:O 22:CB:883:LEU:HB2 2.16 0.45 25:E4:230:UNK:HA 25:E4:244:UNK:HA 1.98 0.45 35:SC:88:VAL:HG13 35:SC:96:LEU:HD11 1.99 0.45 39:SI:21:ALA:HA 39:SI:24:PHE:HD2 1.82 0.45 46:SR:42:GLU:CG 46:SR:45:ARG:HH12 2.26 0.45 46:SR:24:ALA:HA 46:SR:63:ILE:HA 1.99 0.45 8:AA:478:UNK:CB 8:AA:482:UNK:O 2.65 0.45 12:AE:814:UNK:O 12:AE:818:UNK:CB 2.65 0.45 14:AG:122:UNK:N 14:AG:126:UNK:O 2.50 0.45 15:B1:893:ASN:HD22 15:B1:919:VAL:HG12 1.81 0.45 16:BA:385:GLU:OE1 16:BA:421:ASN:ND2 2.49 0.45 20:BE:36:GLY:HA2 20:BE:299:THR:HG21 1.99 0.45 20:BE:418:ALA:HB1 20:BE:468:MET:HG3 1.98 0.45 32:R2:1613:PRO:HD2 32:R2:1616:ASN:HD22 1.82 0.45 34:SA:1473:U:C2 37:SG:102:ARG:NH1 2.85 0.45 34:SA:1638:G:H22 34:SA:1763:A:H2 1.64 0.45 44:SO:114:ARG:HG3 44:SO:117:LEU:HD12 1.99 0.45 15:B1:143:MET:H 48:SY:144:ARG:HH11 1.64 0.45 1:3A:199:G:C2 5:3F:153:GLY:HA2 2.52 0.44 6:3H:84:ARG:HG3 6:3H:96:PRO:HB3 1.98 0.44 12:AE:10:GLN:O 12:AE:13:SER:OG 2.30 0.44 13:AF:505:UNK:O 13:AF:509:UNK:CB 2.65 0.44 15:B1:977:LYS:HB3 15:B1:988:ARG:HG3 1.99 0.44 16:BA:710:ILE:HA 20:BE:596:GLU:HB2 1.98 0.44 17:BB:478:SER:H 17:BB:491:GLY:HA3 1.81 0.44 18:BC:308:ASP:N 18:BC:308:ASP:OD1 2.51 0.44 18:BC:36:VAL:HB 18:BC:39:GLU:H 1.83 0.44 20:BE:666:ARG:HB3 20:BE:667:THR:H 1.64 0.44 20:BE:860:GLU:HA 20:BE:863:TRP:HE3 1.82 0.44 22:CB:970:TRP:HZ3 22:CB:1034:GLY:HA3 1.83 0.44 22:CB:315:ILE:HB 22:CB:553:VAL:HG13 1.99 0.44 23:E2:121:LEU:HD11 23:E2:167:ILE:HD13 1.99 0.44 25:E4:59:UNK:O 25:E4:68:UNK:N 2.51 0.44 34:SA:156:A:H2’ 34:SA:157:A:C8 2.52 0.44 Continued on next page... Page 108 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:568:G:N2 34:SA:569:C:N3 2.65 0.44 43:SN:67:THR:HG22 43:SN:68:GLU:HG3 1.99 0.44 61:UC:731:UNK:HA 61:UC:732:UNK:HA 1.74 0.44 6:3H:85:VAL:HG22 6:3H:96:PRO:HG3 1.99 0.44 7:5A:163:G:H2’ 7:5A:164:G:C8 2.52 0.44 8:AA:36:UNK:O 8:AA:44:UNK:N 2.50 0.44 11:AD:411:UNK:O 11:AD:415:UNK:CB 2.65 0.44 12:AE:1070:UNK:O 12:AE:1074:UNK:CB 2.65 0.44 18:BC:213:SER:OG 18:BC:223:TRP:NE1 2.45 0.44 20:BE:349:HIS:ND1 20:BE:374:SER:OG 2.38 0.44 22:CB:643:ALA:O 22:CB:647:ASN:HB2 2.17 0.44 23:E1:98:THR:OG1 23:E1:235:SER:OG 2.30 0.44 23:E2:103:PRO:O 23:E2:107:ALA:N 2.42 0.44 23:E2:151:ARG:NH2 34:SA:1572:G:OP2 2.50 0.44 34:SA:1145:U:H3 34:SA:1633:A:H2’ 1.82 0.44 42:SM:75:VAL:HG22 42:SM:86:ILE:HG22 1.99 0.44 54:U1:157:UNK:HA 54:U1:172:UNK:HA 1.99 0.44 57:U4:72:ILE:HG23 57:U4:82:ILE:HD11 1.99 0.44 2:3B:116:SER:HB3 2:3B:122:ARG:HH12 1.82 0.44 2:3B:117:VAL:HG11 2:3B:179:THR:HA 1.98 0.44 7:5A:281:G:H2’ 7:5A:282:G:H8 1.82 0.44 10:AC:8:UNK:N 10:AC:281:UNK:O 2.50 0.44 12:AE:659:LEU:HD23 12:AE:692:ILE:HA 1.99 0.44 12:AE:769:LEU:HB3 12:AE:801:ILE:HG12 2.00 0.44 13:AF:427:UNK:O 13:AF:431:UNK:CB 2.65 0.44 19:BD:423:LEU:HG 19:BD:432:VAL:HG13 1.99 0.44 19:BD:424:ILE:HD13 19:BD:433:TRP:HD1 1.81 0.44 20:BE:292:ARG:NH2 20:BE:350:SER:OG 2.46 0.44 20:BE:602:SER:O 20:BE:609:ILE:HA 2.17 0.44 16:BA:85:LYS:HE2 20:BE:659:GLN:HA 1.98 0.44 21:CA:211:GLY:HA2 21:CA:214:LEU:HD12 1.99 0.44 22:CB:864:LEU:HD23 22:CB:867:ILE:HD12 1.98 0.44 24:E3:251:PRO:HA 24:E3:254:TRP:CE2 2.52 0.44 30:P1:161:ALA:HA 30:P1:232:HIS:HE1 1.83 0.44 34:SA:10:G:H2’ 34:SA:11:A:C8 2.52 0.44 36:SF:100:ARG:HH12 36:SF:236:ILE:HG21 1.81 0.44 46:SR:23:LYS:O 46:SR:64:ASP:N 2.43 0.44 61:UC:58:UNK:O 61:UC:62:UNK:N 2.50 0.44 10:AC:466:UNK:O 10:AC:470:UNK:CB 2.66 0.44 10:AC:59:UNK:O 10:AC:68:UNK:N 2.51 0.44 15:B1:106:ALA:HA 15:B1:109:ASP:HB2 1.99 0.44 Continued on next page... Page 109 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 17:BB:357:THR:OG1 17:BB:361:THR:O 2.34 0.44 18:BC:172:VAL:HB 18:BC:186:LEU:HB2 2.00 0.44 24:E3:276:GLN:O 24:E3:280:ASN:HB2 2.18 0.44 25:E4:199:UNK:O 25:E4:212:UNK:CB 2.66 0.44 26:K1:132:VAL:HG11 26:K1:137:ARG:HB3 1.98 0.44 26:K1:140:LYS:HB3 26:K1:144:ARG:HH22 1.82 0.44 34:SA:924:A:H2’ 34:SA:925:G:C4 2.52 0.44 35:SC:83:LYS:HZ1 35:SC:106:THR:HA 1.82 0.44 35:SC:83:LYS:NZ 35:SC:106:THR:HA 2.33 0.44 39:SI:30:SER:HB2 39:SI:34:LEU:HB2 1.99 0.44 41:SK:78:ARG:HH11 41:SK:81:VAL:HG21 1.83 0.44 57:U4:71:PHE:HB2 57:U4:155:THR:HA 1.99 0.44 5:3F:440:HIS:HA 5:3F:497:LYS:HE3 2.00 0.44 7:5A:101:G:N2 7:5A:102:A:N1 2.61 0.44 14:AG:132:UNK:HA 14:AG:133:UNK:HA 1.73 0.44 16:BA:445:VAL:HG13 16:BA:458:TRP:HB2 2.00 0.44 28:MB:249:GLU:HB3 28:MB:253:ARG:HE 1.82 0.44 33:S1:137:UNK:O 33:S1:145:UNK:N 2.50 0.44 33:S1:179:UNK:HA 33:S1:185:UNK:HA 1.99 0.44 35:SC:129:THR:OG1 35:SC:131:ASP:O 2.29 0.44 46:SR:113:ASP:OD1 46:SR:116:LEU:N 2.46 0.44 46:SR:58:ASP:O 46:SR:60:PHE:N 2.51 0.44 6:3H:56:ALA:HB3 6:3H:82:PRO:HA 2.00 0.44 7:5A:290:G:H2’ 7:5A:291:G:H8 1.83 0.44 8:AA:148:UNK:O 8:AA:159:UNK:HA 2.18 0.44 12:AE:1651:UNK:HA 12:AE:1659:UNK:HA 1.99 0.44 14:AG:284:UNK:CB 14:AG:293:UNK:O 2.66 0.44 34:SA:1216:C:O2’ 34:SA:1217:A:OP1 2.35 0.44 34:SA:1650:U:H2’ 34:SA:1651:A:H8 1.83 0.44 35:SC:97:LEU:HD22 35:SC:232:HIS:CE1 2.52 0.44 37:SG:92:ARG:HA 37:SG:95:ASN:HD22 1.82 0.44 42:SM:130:PRO:HA 42:SM:136:ARG:HG3 1.99 0.44 58:U5:147:SER:HA 58:U5:148:LYS:HA 1.67 0.44 10:AC:598:UNK:O 10:AC:602:UNK:CB 2.66 0.44 12:AE:528:ARG:HA 12:AE:531:ILE:HG22 1.99 0.44 12:AE:564:VAL:HB 12:AE:614:LEU:HD11 2.00 0.44 12:AE:912:UNK:HA 12:AE:913:UNK:HA 1.78 0.44 16:BA:423:ARG:NH2 16:BA:461:GLN:O 2.50 0.44 18:BC:35:PRO:HG3 18:BC:67:LEU:HG 1.99 0.44 19:BD:445:ARG:NH1 19:BD:499:ALA:O 2.50 0.44 21:CA:185:HIS:O 21:CA:189:ALA:CB 2.66 0.44 Continued on next page... Page 110 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 25:E4:3:UNK:O 25:E4:285:UNK:N 2.51 0.44 34:SA:361:C:N4 34:SA:384:G:O6 2.51 0.44 57:U4:63:TYR:O 57:U4:95:ASN:N 2.50 0.44 2:3B:264:GLN:HE22 2:3B:322:ARG:HA 1.81 0.44 5:3F:442:ILE:HD12 5:3F:470:LEU:HD12 1.99 0.44 7:5A:168:G:H1 7:5A:228:A:N6 2.16 0.44 15:B1:957:GLU:HG2 15:B1:960:ARG:HH11 1.82 0.44 16:BA:45:ASN:HB2 16:BA:377:GLY:HA3 1.99 0.44 18:BC:348:PRO:HB3 18:BC:399:ASP:HA 1.99 0.44 22:CB:1138:ASP:O 22:CB:1141:GLU:HB2 2.18 0.44 23:E2:67:LEU:HD11 23:E2:141:MET:HB2 1.98 0.44 30:P1:248:VAL:HA 30:P1:251:ILE:HD12 2.00 0.44 1:3A:18:G:H2’ 1:3A:19:A:H8 1.82 0.44 5:3F:342:ARG:NH1 6:3G:89:ARG:O 2.51 0.44 8:AA:231:UNK:HA 8:AA:240:UNK:O 2.17 0.44 10:AC:548:UNK:O 10:AC:552:UNK:CB 2.66 0.44 12:AE:854:UNK:O 12:AE:858:UNK:CB 2.66 0.44 13:AF:428:UNK:O 13:AF:432:UNK:CB 2.66 0.44 19:BD:429:TYR:HD2 19:BD:431:GLU:HG2 1.81 0.44 20:BE:154:TYR:OH 20:BE:194:ARG:O 2.25 0.44 20:BE:230:VAL:HB 20:BE:244:ILE:HB 2.00 0.44 21:CA:146:ASN:HB3 21:CA:150:LYS:NZ 2.32 0.44 24:E3:260:TYR:HA 24:E3:306:ALA:HB2 1.99 0.44 28:MB:236:HIS:HE1 28:MB:249:GLU:HG2 1.83 0.44 24:E3:305:ARG:HA 34:SA:1217:A:C8 2.52 0.44 36:SF:136:VAL:HG21 36:SF:148:ARG:NH1 2.33 0.44 40:SJ:67:TRP:NE1 40:SJ:69:SER:OG 2.51 0.44 58:U5:213:LEU:HA 58:U5:216:ILE:HD12 1.98 0.44 1:3A:310:G:H2’ 1:3A:311:G:H8 1.83 0.43 4:3E:277:SER:HA 4:3E:293:GLU:OE2 2.18 0.43 5:3F:367:LYS:HA 5:3F:370:ARG:HD3 2.00 0.43 15:B1:868:ARG:HH21 15:B1:928:ILE:HA 1.83 0.43 20:BE:291:HIS:HD2 20:BE:298:VAL:HG22 1.83 0.43 20:BE:38:PRO:HB3 20:BE:300:GLN:HE21 1.82 0.43 20:BE:412:PRO:O 20:BE:434:HIS:ND1 2.42 0.43 23:E2:219:GLU:HA 37:SG:157:ARG:HH22 1.83 0.43 24:E3:457:ARG:HE 24:E3:461:LEU:HD11 1.83 0.43 31:R1:301:MET:HG2 31:R1:354:ILE:HD13 2.01 0.43 34:SA:1585:U:H2’ 34:SA:1586:A:H8 1.83 0.43 34:SA:51:A:N6 34:SA:429:G:H21 2.14 0.43 61:UC:69:UNK:O 61:UC:73:UNK:N 2.51 0.43 Continued on next page... Page 111 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 9:AB:244:UNK:N 9:AB:248:UNK:O 2.51 0.43 14:AG:79:UNK:CB 14:AG:88:UNK:O 2.66 0.43 16:BA:603:LEU:HB2 16:BA:612:LEU:O 2.18 0.43 17:BB:219:THR:HG22 17:BB:227:LYS:HB2 2.00 0.43 17:BB:253:LYS:NZ 17:BB:254:GLN:O 2.48 0.43 17:BB:444:LEU:HD13 17:BB:456:LEU:HD11 1.99 0.43 18:BC:270:ILE:HA 18:BC:277:VAL:HA 2.01 0.43 18:BC:311:LEU:HD12 18:BC:333:ILE:HD11 2.01 0.43 27:MA:114:ILE:HG13 27:MA:150:VAL:HB 2.00 0.43 34:SA:1628:U:H2’ 34:SA:1629:G:H8 1.82 0.43 34:SA:894:U:H2’ 34:SA:895:G:C8 2.54 0.43 39:SI:159:VAL:HG23 39:SI:163:ASP:OD2 2.17 0.43 15:B1:828:ARG:HD3 48:SY:94:ASN:HA 2.00 0.43 61:UC:771:UNK:O 61:UC:775:UNK:N 2.51 0.43 9:AB:148:UNK:HA 9:AB:161:UNK:O 2.17 0.43 10:AC:235:UNK:CB 10:AC:239:UNK:O 2.66 0.43 10:AC:240:UNK:O 10:AC:253:UNK:CB 2.66 0.43 12:AE:121:LEU:HB3 12:AE:127:ILE:HD13 2.00 0.43 12:AE:595:THR:HG22 12:AE:619:SER:HA 2.00 0.43 13:AF:100:UNK:O 13:AF:109:UNK:N 2.51 0.43 13:AF:153:UNK:CB 13:AF:157:UNK:O 2.66 0.43 14:AG:264:UNK:HA 14:AG:274:UNK:O 2.17 0.43 14:AG:841:UNK:O 14:AG:845:UNK:CB 2.66 0.43 16:BA:99:CYS:SG 16:BA:115:SER:OG 2.76 0.43 16:BA:347:SER:HB2 16:BA:698:THR:HA 2.01 0.43 16:BA:522:SER:HB2 16:BA:583:ILE:HG23 1.99 0.43 17:BB:634:SER:OG 17:BB:635:LYS:N 2.49 0.43 17:BB:857:LEU:HD21 17:BB:884:LYS:HE2 2.01 0.43 22:CB:364:VAL:HA 22:CB:367:ARG:HG2 1.99 0.43 22:CB:412:LEU:HD22 22:CB:421:LEU:HA 2.01 0.43 22:CB:506:GLN:OE1 22:CB:509:ASN:ND2 2.48 0.43 30:P1:188:LYS:HD2 30:P1:191:LYS:NZ 2.34 0.43 33:S1:282:UNK:O 33:S1:291:UNK:N 2.51 0.43 34:SA:283:U:H5” 38:SH:188:ARG:NE 2.31 0.43 34:SA:328:A:H2’ 34:SA:329:G:C8 2.53 0.43 34:SA:363:G:H2’ 34:SA:364:G:H8 1.83 0.43 34:SA:975:C:N4 34:SA:976:G:O6 2.52 0.43 36:SF:100:ARG:NH1 36:SF:236:ILE:HG21 2.34 0.43 44:SO:85:PRO:HD2 44:SO:88:LEU:HD13 2.00 0.43 58:U5:86:THR:HA 58:U5:90:ASP:HB2 2.00 0.43 2:3B:271:ILE:O 2:3B:313:HIS:HA 2.19 0.43 Continued on next page... Page 112 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 15:B1:819:GLU:O 15:B1:852:ARG:NH1 2.52 0.43 16:BA:308:VAL:HA 16:BA:319:PHE:HA 2.01 0.43 16:BA:475:PRO:HD2 16:BA:493:TRP:HB3 2.01 0.43 16:BA:498:ARG:HB3 16:BA:500:TRP:HE1 1.84 0.43 17:BB:166:ILE:HD11 17:BB:179:SER:HB2 1.99 0.43 17:BB:622:LYS:HB2 17:BB:631:PHE:CZ 2.54 0.43 18:BC:492:LYS:HD3 18:BC:511:TYR:HD2 1.83 0.43 20:BE:627:ASN:ND2 20:BE:647:THR:O 2.41 0.43 23:E2:53:HIS:N 23:E2:66:VAL:O 2.51 0.43 33:S1:289:UNK:HA 33:S1:303:UNK:HA 2.01 0.43 34:SA:1679:G:N2 34:SA:1723:U:O4 2.51 0.43 34:SA:1802:A:N6 34:SA:1808:U:O4 2.50 0.43 34:SA:491:C:H2’ 34:SA:492:A:C8 2.53 0.43 35:SC:127:VAL:HG11 35:SC:176:VAL:HG11 2.01 0.43 37:SG:81:ARG:NH1 37:SG:82:PHE:HE2 2.16 0.43 38:SH:57:ASP:HA 38:SH:106:LEU:HA 2.01 0.43 5:3F:491:SER:OG 5:3F:492:TRP:N 2.51 0.43 7:5A:244:U:H3 7:5A:269:G:H1 1.66 0.43 7:5A:409:C:H2’ 7:5A:410:A:C8 2.53 0.43 15:B1:236:LEU:HB3 15:B1:239:THR:HG23 1.99 0.43 17:BB:385:ILE:HG23 17:BB:417:TRP:HH2 1.83 0.43 24:E3:301:TYR:O 24:E3:304:TYR:CA 2.66 0.43 28:MB:215:LYS:NZ 34:SA:1625:C:H5’ 2.33 0.43 34:SA:1210:C:H2’ 34:SA:1211:A:H8 1.83 0.43 17:BB:163:LYS:HD3 34:SA:1765:A:H4’ 2.01 0.43 34:SA:886:U:H2’ 34:SA:887:A:C8 2.53 0.43 36:SF:149:TYR:HB3 38:SH:208:TYR:CZ 2.52 0.43 40:SJ:4:SER:OG 40:SJ:6:ASP:OD1 2.34 0.43 54:U1:200:UNK:HA 54:U1:211:UNK:HA 2.01 0.43 4:3E:45:ALA:HB1 4:3E:83:LYS:NZ 2.33 0.43 12:AE:272:LYS:HA 12:AE:275:ILE:HD12 2.00 0.43 12:AE:367:LEU:O 12:AE:371:LYS:CB 2.63 0.43 12:AE:894:UNK:O 12:AE:898:UNK:CB 2.66 0.43 18:BC:622:ALA:H 18:BC:635:ALA:HB3 1.84 0.43 19:BD:368:THR:HA 19:BD:385:ASN:O 2.18 0.43 20:BE:97:LYS:NZ 20:BE:111:GLU:HB2 2.30 0.43 20:BE:208:GLN:O 20:BE:226:VAL:N 2.49 0.43 34:SA:273:G:C6 34:SA:283:U:O2 2.72 0.43 34:SA:363:G:H2’ 34:SA:364:G:C8 2.54 0.43 36:SF:92:LEU:HB2 36:SF:97:GLU:HB2 2.01 0.43 39:SI:67:LEU:HG 39:SI:71:HIS:CE1 2.54 0.43 Continued on next page... Page 113 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 45:SP:20:TYR:HB3 45:SP:27:PHE:HB2 2.00 0.43 46:SR:79:TYR:HD1 46:SR:82:ARG:HD3 1.84 0.43 54:U1:77:UNK:HA 54:U1:88:UNK:HA 2.00 0.43 61:UC:243:UNK:O 61:UC:247:UNK:N 2.52 0.43 61:UC:437:UNK:O 61:UC:441:UNK:N 2.52 0.43 1:3A:47:G:H2’ 1:3A:48:A:C8 2.53 0.43 1:3A:84:U:H2’ 1:3A:85:G:H8 1.84 0.43 3:3D:150:ALA:O 3:3D:154:LEU:HB2 2.18 0.43 8:AA:65:UNK:HA 8:AA:78:UNK:O 2.18 0.43 15:B1:836:LYS:H 15:B1:912:ARG:HH12 1.67 0.43 16:BA:412:ARG:HA 16:BA:423:ARG:O 2.19 0.43 22:CB:812:GLU:O 22:CB:816:ALA:CB 2.67 0.43 30:P1:255:PRO:HA 30:P1:256:PRO:HD3 1.88 0.43 31:R1:67:ILE:HG23 31:R1:74:VAL:HG22 2.01 0.43 34:SA:1263:G:C2 34:SA:1264:G:H1’ 2.53 0.43 34:SA:1661:U:H2’ 34:SA:1662:G:C8 2.54 0.43 34:SA:389:G:H3’ 34:SA:390:G:H21 1.83 0.43 35:SC:83:LYS:HB2 35:SC:104:ASP:HB3 2.01 0.43 36:SF:137:PRO:HG2 36:SF:149:TYR:HA 2.00 0.43 7:5A:274:C:H2’ 7:5A:275:A:C8 2.54 0.43 7:5A:379:A:H2’ 7:5A:380:A:C8 2.54 0.43 8:AA:386:UNK:N 8:AA:391:UNK:O 2.51 0.43 15:B1:189:VAL:HG13 15:B1:193:ARG:HA 2.01 0.43 16:BA:66:GLN:HG3 16:BA:68:THR:HG22 2.00 0.43 18:BC:353:LEU:HD12 18:BC:366:PRO:HG2 2.00 0.43 21:CA:6:ILE:HD13 22:CB:1206:PRO:HB2 2.00 0.43 22:CB:334:PHE:O 22:CB:338:SER:OG 2.26 0.43 22:CB:896:THR:O 22:CB:900:GLU:HB2 2.19 0.43 24:E3:228:LYS:NZ 34:SA:1245:G:OP2 2.51 0.43 34:SA:1679:G:H1’ 34:SA:1722:A:H61 1.84 0.43 34:SA:523:G:H22 34:SA:528:U:H5” 1.83 0.43 35:SC:91:VAL:HG23 35:SC:96:LEU:HB2 2.01 0.43 36:SF:12:LEU:HD21 36:SF:22:LYS:HG2 2.01 0.43 40:SJ:8:ARG:HH11 40:SJ:19:ALA:HB3 1.84 0.43 2:3C:169:LYS:O 2:3C:238:ASP:N 2.42 0.43 15:B1:66:ARG:HH21 15:B1:78:ASN:HB3 1.83 0.43 18:BC:614:ASP:OD1 18:BC:614:ASP:N 2.50 0.43 22:CB:298:PHE:HB2 22:CB:340:SER:HA 2.00 0.43 22:CB:644:VAL:O 22:CB:648:PHE:HB2 2.19 0.43 22:CB:823:SER:HA 22:CB:841:ASN:O 2.19 0.43 23:E1:181:SER:OG 23:E1:214:ASP:OD2 2.25 0.43 Continued on next page... Page 114 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 28:MB:173:ARG:HD3 28:MB:252:PRO:HB3 2.00 0.43 32:R2:1690:PHE:HA 32:R2:1693:ASN:HD22 1.83 0.43 34:SA:205:U:H2’ 34:SA:206:A:H8 1.84 0.43 37:SG:94:THR:HG22 37:SG:114:ILE:HG13 2.01 0.43 39:SI:26:GLU:OE2 39:SI:27:LEU:HG 2.19 0.43 40:SJ:85:PRO:HG3 42:SM:12:ALA:HB2 2.01 0.43 44:SO:101:HIS:HA 44:SO:104:ARG:HH11 1.83 0.43 2:3B:227:PRO:HA 2:3B:230:TYR:CE1 2.54 0.43 3:3D:62:ALA:O 3:3D:66:ALA:CB 2.67 0.43 4:3E:160:ASP:OD1 4:3E:163:ILE:HD12 2.18 0.43 3:3D:284:TYR:HE1 4:3E:257:ALA:HB1 1.84 0.43 7:5A:242:C:H2’ 7:5A:243:A:C8 2.53 0.43 16:BA:114:ALA:HB1 16:BA:149:ILE:HB 2.01 0.43 16:BA:556:ARG:HB3 16:BA:557:LYS:H 1.70 0.43 17:BB:438:PHE:HA 17:BB:445:VAL:HG23 2.00 0.43 17:BB:576:VAL:HA 17:BB:592:SER:HA 2.01 0.43 18:BC:18:GLY:O 18:BC:336:ASN:N 2.52 0.43 18:BC:439:ALA:HB3 18:BC:457:ALA:HB3 2.01 0.43 20:BE:375:LEU:HB3 20:BE:389:MET:HB2 2.00 0.43 20:BE:391:GLN:HG2 20:BE:443:TRP:HH2 1.84 0.43 20:BE:588:ILE:HA 20:BE:604:SER:HA 2.01 0.43 22:CB:1157:TYR:HB3 22:CB:1161:LEU:HB2 2.01 0.43 22:CB:758:SER:O 22:CB:762:TYR:N 2.52 0.43 25:E4:33:UNK:HA 25:E4:54:UNK:HA 2.01 0.43 26:K1:125:VAL:O 45:SP:92:LYS:NZ 2.44 0.43 26:K1:99:ASP:O 26:K1:103:LEU:N 2.44 0.43 34:SA:1041:G:H2’ 34:SA:1042:G:C8 2.54 0.43 16:BA:505:ARG:HH22 34:SA:1618:C:H41 1.66 0.43 39:SI:71:HIS:HD2 39:SI:131:PHE:CZ 2.36 0.43 1:3A:79:G:C5 6:3H:95:ARG:HD3 2.54 0.42 3:3D:18:PHE:CZ 3:3D:151:GLN:HB2 2.54 0.42 3:3D:158:TYR:O 3:3D:162:LYS:CB 2.62 0.42 4:3E:58:GLU:HA 4:3E:61:ASN:HD22 1.84 0.42 7:5A:133:U:H2’ 7:5A:134:A:C8 2.54 0.42 7:5A:271:G:H2’ 7:5A:272:A:C8 2.54 0.42 12:AE:576:ILE:HA 12:AE:579:ARG:HE 1.83 0.42 13:AF:143:UNK:N 13:AF:148:UNK:O 2.52 0.42 20:BE:559:ALA:HA 20:BE:569:VAL:HA 2.01 0.42 20:BE:592:ASP:OD1 20:BE:593:PHE:N 2.51 0.42 23:E1:105:ASN:HB2 23:E1:110:LEU:HD23 2.00 0.42 29:MC:429:GLU:N 29:MC:432:LYS:HZ2 2.16 0.42 Continued on next page... Page 115 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 31:R1:177:PRO:HD2 31:R1:304:GLY:HA2 2.00 0.42 34:SA:1606:C:H2’ 34:SA:1607:G:C8 2.54 0.42 34:SA:891:A:H2’ 34:SA:892:A:H8 1.83 0.42 35:SC:36:SER:O 35:SC:38:PHE:N 2.51 0.42 37:SG:142:PRO:HA 37:SG:218:GLU:OE2 2.18 0.42 37:SG:80:LYS:HZ3 37:SG:83:ARG:HH11 1.67 0.42 41:SK:66:ASP:O 41:SK:70:LEU:N 2.49 0.42 34:SA:878:G:O2’ 44:SO:108:ASP:OD1 2.36 0.42 61:UC:769:UNK:O 61:UC:773:UNK:N 2.52 0.42 2:3C:100:ARG:HA 2:3C:101:GLY:HA2 1.77 0.42 5:3F:323:ASP:OD2 5:3F:342:ARG:HD3 2.20 0.42 5:3F:345:THR:HG22 5:3F:360:ARG:HG3 2.01 0.42 12:AE:506:TYR:O 12:AE:510:SER:CB 2.67 0.42 12:AE:816:UNK:O 12:AE:820:UNK:CB 2.67 0.42 15:B1:245:ASP:HB3 15:B1:797:PRO:HA 2.00 0.42 15:B1:846:VAL:HB 15:B1:899:VAL:HG23 2.00 0.42 17:BB:656:HIS:CE1 17:BB:682:ARG:HD2 2.55 0.42 17:BB:15:GLY:H 17:BB:685:GLU:HB3 1.84 0.42 25:E4:258:UNK:HA 40:SJ:42:ARG:HH11 1.81 0.42 31:R1:153:THR:HA 31:R1:166:VAL:HG12 2.00 0.42 33:S1:101:UNK:N 33:S1:385:UNK:O 2.48 0.42 34:SA:497:G:H2’ 34:SA:498:G:C8 2.54 0.42 36:SF:211:LYS:HB3 36:SF:217:THR:HG22 2.01 0.42 44:SO:55:ARG:HA 44:SO:60:VAL:HB 2.01 0.42 47:SX:65:LEU:HD22 47:SX:66:ASN:H 1.85 0.42 1:3A:330:A:H2’ 1:3A:331:A:C8 2.54 0.42 2:3C:180:SER:HA 2:3C:183:HIS:HD2 1.83 0.42 8:AA:27:UNK:O 8:AA:34:UNK:HA 2.19 0.42 8:AA:404:UNK:HA 8:AA:410:UNK:HA 2.02 0.42 12:AE:1762:UNK:O 12:AE:1766:UNK:CB 2.67 0.42 14:AG:8:UNK:N 14:AG:567:UNK:O 2.51 0.42 18:BC:397:THR:OG1 18:BC:398:GLU:N 2.53 0.42 18:BC:391:LEU:HD23 18:BC:407:SER:HB2 2.00 0.42 20:BE:323:VAL:HG13 20:BE:343:LEU:HB2 2.00 0.42 22:CB:543:LEU:HG 22:CB:545:ALA:H 1.85 0.42 27:MA:111:LEU:HB2 27:MA:148:TYR:HB3 2.02 0.42 34:SA:1049:U:H2’ 34:SA:1050:G:C8 2.54 0.42 34:SA:1179:G:O6 34:SA:1461:C:N4 2.53 0.42 35:SC:97:LEU:HD22 35:SC:232:HIS:HE1 1.84 0.42 36:SF:129:VAL:HB 36:SF:139:VAL:HG12 2.01 0.42 36:SF:137:PRO:HB2 36:SF:150:PRO:HD2 2.01 0.42 Continued on next page... Page 116 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:283:U:O4’ 38:SH:188:ARG:HG3 2.19 0.42 41:SK:58:ASP:O 41:SK:61:THR:OG1 2.32 0.42 58:U5:64:LEU:HB3 58:U5:68:ARG:HE 1.84 0.42 7:5A:251:C:H2’ 7:5A:252:A:C8 2.54 0.42 12:AE:687:LEU:HB2 12:AE:691:PHE:HD2 1.83 0.42 15:B1:962:GLU:HA 15:B1:976:ILE:HB 2.01 0.42 16:BA:536:LYS:HG3 16:BA:538:GLN:H 1.85 0.42 16:BA:99:CYS:HG 16:BA:115:SER:HG 1.67 0.42 19:BD:362:HIS:H 19:BD:418:LYS:HD3 1.85 0.42 21:CA:138:TRP:CD1 21:CA:142:LYS:HE3 2.54 0.42 23:E1:122:ILE:HA 23:E1:161:LYS:O 2.19 0.42 24:E3:308:LYS:HB3 24:E3:345:VAL:HG21 2.01 0.42 28:MB:177:ILE:HG21 34:SA:1159:C:H5’ 2.02 0.42 32:R2:1582:VAL:HG13 32:R2:1620:VAL:HG12 2.00 0.42 34:SA:1482:C:O4’ 46:SR:74:HIS:NE2 2.52 0.42 34:SA:162:A:OP2 34:SA:162:A:H8 2.00 0.42 34:SA:139:C:H42 34:SA:175:G:H21 1.67 0.42 34:SA:200:A:H2’ 34:SA:201:G:H8 1.83 0.42 34:SA:890:C:H2’ 34:SA:891:A:H8 1.84 0.42 34:SA:868:G:H1 34:SA:960:U:H3 1.67 0.42 46:SR:52:LEU:C 46:SR:59:LYS:HZ1 2.20 0.42 48:SY:126:LYS:HG2 48:SY:131:SER:HA 2.01 0.42 7:5A:182:G:H2’ 7:5A:183:A:H8 1.85 0.42 9:AB:121:UNK:N 9:AB:125:UNK:O 2.52 0.42 9:AB:54:UNK:O 9:AB:72:UNK:N 2.53 0.42 12:AE:132:THR:HG23 12:AE:155:ILE:HD11 2.01 0.42 16:BA:36:ARG:NH1 16:BA:699:GLU:OE2 2.53 0.42 16:BA:716:ASP:O 16:BA:744:ARG:NH2 2.46 0.42 17:BB:857:LEU:HD12 17:BB:860:ILE:HD11 2.02 0.42 18:BC:343:MET:HB2 18:BC:353:LEU:HD21 2.01 0.42 18:BC:345:TYR:CD2 18:BC:350:LEU:HD12 2.54 0.42 18:BC:532:HIS:CG 18:BC:536:LEU:HD11 2.55 0.42 18:BC:47:PRO:HD3 18:BC:53:LEU:HG 2.01 0.42 20:BE:914:ASP:OD1 20:BE:914:ASP:N 2.52 0.42 22:CB:712:ASN:HA 22:CB:1115:LEU:HG 2.00 0.42 22:CB:752:SER:HB3 22:CB:754:LEU:HG 2.01 0.42 26:K1:195:HIS:HA 26:K1:196:PRO:HD3 1.88 0.42 34:SA:1573:A:H4’ 34:SA:1574:G:H5’ 2.01 0.42 34:SA:340:U:H2’ 34:SA:341:A:H8 1.84 0.42 34:SA:1253:U:O5’ 43:SN:46:ARG:NH2 2.51 0.42 35:SC:77:GLU:OE2 45:SP:114:ARG:NH2 2.53 0.42 Continued on next page... Page 117 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 7:5A:125:G:H2’ 7:5A:126:A:C8 2.54 0.42 7:5A:26:C:N4 7:5A:54:A:OP2 2.52 0.42 8:AA:99:UNK:O 8:AA:108:UNK:N 2.51 0.42 12:AE:1506:UNK:HA 12:AE:1507:UNK:HA 1.80 0.42 12:AE:33:LEU:HD12 12:AE:115:LEU:HD11 2.01 0.42 12:AE:405:GLU:HA 12:AE:414:LEU:HD13 2.02 0.42 18:BC:13:ASN:N 18:BC:643:PHE:O 2.53 0.42 20:BE:140:TYR:HE1 20:BE:153:PHE:HD1 1.66 0.42 22:CB:559:GLU:OE2 22:CB:608:PHE:HB3 2.20 0.42 23:E1:28:PRO:HA 23:E1:29:PRO:HD3 1.88 0.42 34:SA:1158:C:N3 34:SA:1581:C:H3’ 2.35 0.42 34:SA:153:G:H2’ 34:SA:154:G:C8 2.54 0.42 34:SA:283:U:N3 38:SH:188:ARG:CB 2.75 0.42 34:SA:891:A:H2’ 34:SA:892:A:C8 2.54 0.42 35:SC:207:LEU:HD22 35:SC:210:ILE:HD11 2.01 0.42 46:SR:44:LEU:HD12 46:SR:47:LYS:HG3 2.01 0.42 54:U1:13:UNK:O 54:U1:31:UNK:N 2.52 0.42 57:U4:66:LEU:HD13 57:U4:141:LEU:HD22 2.01 0.42 61:UC:445:UNK:O 61:UC:449:UNK:N 2.53 0.42 1:3A:102:U:H2’ 1:3A:103:A:C8 2.54 0.42 1:3A:63:C:H2’ 1:3A:64:A:C8 2.54 0.42 2:3C:251:ARG:HH12 8:AA:249:UNK:HA 1.85 0.42 5:3F:481:ILE:HA 5:3F:482:PRO:HD3 1.88 0.42 6:3H:93:VAL:HG12 6:3H:95:ARG:H 1.84 0.42 7:5A:352:U:H5” 7:5A:353:A:OP2 2.20 0.42 7:5A:556:G:H2’ 7:5A:557:A:C8 2.51 0.42 8:AA:159:UNK:O 8:AA:167:UNK:N 2.53 0.42 12:AE:106:SER:HA 12:AE:109:TRP:HE1 1.84 0.42 12:AE:666:ALA:HA 12:AE:669:VAL:HG12 2.01 0.42 15:B1:134:PHE:HA 15:B1:137:ILE:HD12 2.00 0.42 16:BA:119:LEU:HD23 16:BA:142:HIS:HB2 2.01 0.42 17:BB:126:LEU:N 17:BB:139:GLY:O 2.52 0.42 17:BB:135:ARG:HA 17:BB:148:TRP:O 2.19 0.42 18:BC:33:ALA:H 18:BC:69:LEU:HD21 1.85 0.42 18:BC:667:GLN:HE22 18:BC:688:LEU:HB3 1.85 0.42 19:BD:519:SER:HB2 19:BD:534:SER:HA 2.02 0.42 22:CB:1103:ARG:HD3 22:CB:1179:LYS:HE2 2.02 0.42 22:CB:156:LYS:HD2 22:CB:237:HIS:CE1 2.55 0.42 22:CB:419:TYR:O 22:CB:423:LYS:CB 2.67 0.42 22:CB:883:LEU:HD11 22:CB:1051:LEU:HA 2.02 0.42 24:E3:449:HIS:HB3 24:E3:453:GLY:H 1.85 0.42 Continued on next page... Page 118 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 25:E4:220:UNK:CB 25:E4:234:UNK:O 2.68 0.42 33:S1:126:UNK:N 33:S1:138:UNK:O 2.53 0.42 34:SA:156:A:H1’ 34:SA:416:A:C5 2.54 0.42 34:SA:863:A:N6 34:SA:964:U:C2 2.88 0.42 34:SA:883:C:H2’ 34:SA:884:A:H8 1.83 0.42 36:SF:206:ASP:HB2 36:SF:222:LEU:HB2 2.00 0.42 40:SJ:8:ARG:NH1 40:SJ:19:ALA:H 2.17 0.42 47:SX:76:SER:HA 47:SX:77:PRO:HA 1.92 0.42 1:3A:204:U:H2’ 1:3A:205:G:C8 2.55 0.42 4:3E:210:LEU:HA 4:3E:256:ASN:HD22 1.84 0.42 5:3F:443:LEU:N 5:3F:471:GLN:O 2.46 0.42 5:3F:160:ILE:HG23 5:3F:525:GLN:HG2 2.01 0.42 12:AE:374:ARG:HE 12:AE:404:PHE:HE1 1.66 0.42 15:B1:133:GLU:OE1 15:B1:832:HIS:NE2 2.52 0.42 16:BA:516:SER:HA 16:BA:535:LEU:HB2 2.02 0.42 17:BB:630:PHE:O 17:BB:642:TRP:HB2 2.19 0.42 18:BC:222:LEU:HD12 18:BC:235:LYS:HB2 2.02 0.42 20:BE:342:TYR:OH 20:BE:345:SER:OG 2.36 0.42 22:CB:334:PHE:O 22:CB:338:SER:HB3 2.20 0.42 24:E3:260:TYR:HD1 24:E3:306:ALA:HB2 1.84 0.42 15:B1:634:ARG:HH12 31:R1:185:ARG:HG2 1.85 0.42 32:R2:1473:ASN:HA 32:R2:1505:ILE:HG23 2.00 0.42 32:R2:1476:VAL:O 32:R2:1480:ASN:ND2 2.52 0.42 32:R2:1644:LEU:HB3 32:R2:1654:LEU:HD13 2.02 0.42 34:SA:1042:G:H2’ 34:SA:1043:A:C8 2.55 0.42 34:SA:1726:G:H2’ 34:SA:1727:G:H8 1.84 0.42 34:SA:568:G:N2 34:SA:575:C:N3 2.65 0.42 35:SC:90:GLU:HB2 35:SC:97:LEU:HD21 2.01 0.42 37:SG:77:TYR:HA 37:SG:83:ARG:HE 1.84 0.42 38:SH:98:ARG:HH12 38:SH:103:GLY:N 2.17 0.42 40:SJ:78:ILE:HA 40:SJ:104:ILE:HG22 2.02 0.42 44:SO:71:ILE:HA 44:SO:74:ILE:HD12 2.01 0.42 1:3A:84:U:H2’ 1:3A:85:G:C8 2.55 0.42 6:3G:56:ALA:N 6:3G:81:VAL:O 2.47 0.42 8:AA:29:UNK:CB 8:AA:33:UNK:O 2.68 0.42 15:B1:938:PRO:HB2 15:B1:941:ILE:HD11 2.02 0.42 16:BA:619:ARG:HD3 16:BA:619:ARG:H 1.85 0.42 20:BE:365:PHE:HA 20:BE:378:PHE:O 2.20 0.42 22:CB:781:ASP:N 22:CB:781:ASP:OD1 2.52 0.42 23:E1:196:LEU:HD11 23:E1:202:ILE:HD12 2.02 0.42 32:R2:1633:ASP:HB3 32:R2:1636:ARG:HD3 2.00 0.42 Continued on next page... Page 119 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:1177:C:H2’ 34:SA:1178:G:C8 2.54 0.42 3:3D:54:ALA:HB2 3:3D:81:ILE:HD12 2.02 0.42 10:AC:137:UNK:HA 10:AC:152:UNK:O 2.20 0.42 15:B1:112:LYS:HB3 15:B1:141:HIS:ND1 2.35 0.42 15:B1:155:LEU:HD11 15:B1:190:ILE:HD11 2.02 0.42 15:B1:260:PRO:HG3 15:B1:282:LYS:HD3 2.00 0.42 17:BB:407:ALA:HB1 17:BB:414:LEU:HD11 2.02 0.42 17:BB:439:LEU:HD12 17:BB:440:PRO:HD2 2.01 0.42 18:BC:193:VAL:HG23 18:BC:215:GLY:H 1.85 0.42 23:E1:180:LEU:HD13 23:E1:237:ALA:HB1 2.01 0.42 31:R1:228:ASP:OD2 31:R1:230:TRP:NE1 2.52 0.42 31:R1:9:THR:HB 31:R1:31:ILE:HG22 2.02 0.42 34:SA:1202:A:H4’ 34:SA:1205:C:H42 1.85 0.42 34:SA:1593:A:H2’ 34:SA:1594:G:C8 2.55 0.42 7:5A:476:A:H2’ 7:5A:477:G:C8 2.55 0.41 7:5A:578:G:H2’ 7:5A:579:G:C8 2.55 0.41 15:B1:151:THR:HG22 15:B1:186:LEU:HB2 2.02 0.41 17:BB:255:ARG:HA 17:BB:346:VAL:HG11 2.01 0.41 18:BC:110:ALA:HB3 18:BC:150:LEU:HD11 2.01 0.41 19:BD:363:LEU:H 19:BD:418:LYS:NZ 2.18 0.41 19:BD:495:ASP:H 19:BD:498:ASN:HD22 1.68 0.41 21:CA:46:ASN:HD22 21:CA:47:SER:H 1.68 0.41 22:CB:345:TYR:OH 22:CB:472:THR:OG1 2.32 0.41 34:SA:1059:U:H4’ 34:SA:1060:U:H5’ 2.02 0.41 34:SA:256:A:H2’ 34:SA:257:A:C8 2.54 0.41 34:SA:322:G:O3’ 40:SJ:10:LYS:NZ 2.53 0.41 35:SC:196:GLU:HA 35:SC:199:ASN:HD22 1.85 0.41 38:SH:57:ASP:HA 38:SH:107:ALA:H 1.84 0.41 47:SX:17:ALA:HA 47:SX:22:LYS:HZ1 1.83 0.41 58:U5:82:LEU:HG 58:U5:174:ILE:HG21 2.02 0.41 2:3B:165:ALA:HB3 2:3B:168:LYS:HE2 2.02 0.41 4:3E:44:ILE:HG23 4:3E:127:LEU:HD22 2.02 0.41 1:3A:82:G:OP2 4:3E:365:ALA:HB1 2.20 0.41 7:5A:250:G:N2 7:5A:262:U:O2 2.33 0.41 7:5A:384:U:H2’ 7:5A:385:A:C8 2.55 0.41 12:AE:95:ASP:HA 12:AE:98:ILE:HD12 2.02 0.41 14:AG:475:UNK:HA 14:AG:489:UNK:HA 2.01 0.41 15:B1:63:THR:HG22 15:B1:193:ARG:HE 1.84 0.41 16:BA:111:PHE:HB3 16:BA:122:TRP:O 2.20 0.41 16:BA:743:PHE:CZ 16:BA:777:ARG:HB3 2.55 0.41 18:BC:206:GLU:HA 18:BC:209:LEU:HB2 2.03 0.41 Continued on next page... Page 120 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 18:BC:706:ARG:HH21 18:BC:715:LYS:HA 1.84 0.41 22:CB:130:LYS:HD2 22:CB:283:TYR:CZ 2.56 0.41 25:E4:120:UNK:HA 25:E4:126:UNK:HA 2.02 0.41 3:3D:30:ARG:NH2 33:S1:204:UNK:O 2.53 0.41 34:SA:1748:G:H2’ 34:SA:1749:A:C8 2.54 0.41 34:SA:482:U:H2’ 34:SA:483:A:C8 2.54 0.41 34:SA:947:U:H2’ 34:SA:948:G:C8 2.55 0.41 39:SI:122:HIS:HA 39:SI:125:ILE:HD12 2.02 0.41 39:SI:13:PRO:HA 39:SI:14:THR:HA 1.91 0.41 44:SO:34:ILE:HA 44:SO:37:ILE:HD12 2.01 0.41 5:3F:352:PRO:HB3 49:SZ:10:ARG:NH1 2.34 0.41 54:U1:116:UNK:HA 54:U1:131:UNK:HA 2.01 0.41 6:3H:79:VAL:HG23 6:3H:121:ILE:HG23 2.02 0.41 7:5A:529:A:H2’ 7:5A:530:A:H8 1.85 0.41 9:AB:149:UNK:O 9:AB:160:UNK:HA 2.19 0.41 15:B1:969:VAL:HG22 15:B1:1000:ILE:H 1.84 0.41 16:BA:161:ILE:O 16:BA:172:ILE:HA 2.20 0.41 16:BA:600:SER:OG 16:BA:601:ILE:N 2.51 0.41 20:BE:828:LYS:HA 20:BE:870:GLN:HE22 1.85 0.41 20:BE:906:ALA:O 20:BE:910:GLN:HB2 2.21 0.41 22:CB:201:SER:O 22:CB:290:PHE:HA 2.20 0.41 28:MB:109:LEU:HD12 28:MB:111:PHE:HE1 1.85 0.41 34:SA:576:G:OP2 34:SA:576:G:H8 2.03 0.41 39:SI:14:THR:OG1 39:SI:15:GLU:OE1 2.38 0.41 41:SK:114:TYR:HD1 41:SK:119:ALA:HB3 1.86 0.41 41:SK:38:ASN:HB2 41:SK:41:GLU:HG3 2.02 0.41 47:SX:71:LYS:HB3 47:SX:130:TYR:CZ 2.55 0.41 56:U3:2147:UNK:O 56:U3:2151:UNK:N 2.53 0.41 2:3C:253:ILE:O 2:3C:257:SER:N 2.46 0.41 4:3E:166:ALA:HB1 4:3E:272:LEU:HD11 2.02 0.41 5:3F:485:ASN:O 5:3F:501:ILE:N 2.48 0.41 7:5A:133:U:H2’ 7:5A:134:A:H8 1.84 0.41 13:AF:13:UNK:HA 13:AF:279:UNK:HA 2.03 0.41 14:AG:195:UNK:CB 14:AG:199:UNK:O 2.69 0.41 15:B1:187:SER:O 15:B1:193:ARG:NH2 2.51 0.41 16:BA:557:LYS:HE2 16:BA:598:ASN:HB2 2.02 0.41 17:BB:559:PHE:HE2 17:BB:561:ASP:HB2 1.85 0.41 20:BE:131:SER:HA 20:BE:137:ILE:HG12 2.02 0.41 23:E1:50:LEU:HD12 23:E1:116:THR:HG22 2.03 0.41 31:R1:52:PHE:HE1 31:R1:102:PRO:HG3 1.86 0.41 34:SA:283:U:OP2 38:SH:188:ARG:NE 2.49 0.41 Continued on next page... Page 121 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 35:SC:31:ASP:HB3 35:SC:45:LYS:NZ 2.35 0.41 38:SH:98:ARG:HH12 38:SH:103:GLY:H 1.67 0.41 44:SO:16:ILE:HA 44:SO:17:PRO:HD3 1.91 0.41 1:3A:60:A:H5” 1:3A:61:G:C8 2.55 0.41 3:3D:146:ASP:OD2 4:3E:237:THR:HB 2.21 0.41 5:3F:301:ASP:N 5:3F:301:ASP:OD1 2.53 0.41 7:5A:286:U:H2’ 7:5A:287:G:C8 2.56 0.41 17:BB:50:ASN:ND2 17:BB:52:TRP:HE1 2.17 0.41 22:CB:381:HIS:NE2 22:CB:478:THR:O 2.44 0.41 22:CB:939:ILE:HG12 22:CB:943:VAL:HG13 2.02 0.41 34:SA:1276:U:N3 34:SA:1437:U:O2 2.54 0.41 34:SA:328:A:H2’ 34:SA:329:G:H8 1.85 0.41 36:SF:130:GLN:HB2 36:SF:138:TYR:CZ 2.55 0.41 46:SR:59:LYS:HE2 46:SR:60:PHE:CZ 2.56 0.41 61:UC:297:UNK:O 61:UC:301:UNK:N 2.53 0.41 1:3A:85:G:H2’ 1:3A:86:A:H8 1.85 0.41 7:5A:281:G:H2’ 7:5A:282:G:C8 2.56 0.41 12:AE:1128:UNK:O 12:AE:1133:UNK:N 2.54 0.41 12:AE:345:ASP:HB3 12:AE:375:LEU:HB3 2.02 0.41 12:AE:748:LEU:O 12:AE:752:ALA:CB 2.69 0.41 13:AF:240:UNK:CB 13:AF:253:UNK:O 2.68 0.41 15:B1:560:PRO:HB2 31:R1:320:GLU:HG2 2.02 0.41 17:BB:142:ASP:OD1 17:BB:142:ASP:N 2.54 0.41 19:BD:435:PHE:HZ 19:BD:441:GLY:H 1.68 0.41 20:BE:544:ALA:HB3 20:BE:562:LEU:HD23 2.03 0.41 21:CA:94:GLY:CA 21:CA:121:ARG:HH12 2.34 0.41 22:CB:629:THR:HB 22:CB:667:CYS:HB2 2.01 0.41 23:E1:113:TYR:HE1 23:E1:123:GLU:HG3 1.86 0.41 23:E2:177:LYS:HB3 23:E2:221:VAL:HG12 2.02 0.41 31:R1:192:THR:N 31:R1:248:GLN:HE22 2.18 0.41 33:S1:178:UNK:N 33:S1:187:UNK:O 2.53 0.41 33:S1:282:UNK:N 33:S1:291:UNK:O 2.53 0.41 34:SA:1528:U:P 37:SG:109:LYS:HZ1 2.43 0.41 36:SF:72:VAL:HG12 36:SF:90:ILE:HG12 2.02 0.41 39:SI:129:LEU:HD11 39:SI:173:TYR:HB2 2.02 0.41 39:SI:9:LEU:HD22 39:SI:17:GLU:HB3 2.02 0.41 45:SP:84:ARG:HB2 45:SP:118:VAL:HG23 2.03 0.41 47:SX:86:ILE:HD11 47:SX:117:ARG:NH1 2.36 0.41 54:U1:34:UNK:HA 54:U1:49:UNK:HA 2.02 0.41 3:3D:176:GLN:O 3:3D:180:LEU:HB3 2.20 0.41 4:3E:163:ILE:HG23 4:3E:276:LEU:HD22 2.03 0.41 Continued on next page... Page 122 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 5:3F:156:ASN:HA 5:3F:544:ALA:HB1 2.03 0.41 7:5A:191:U:H2’ 7:5A:192:G:C8 2.56 0.41 12:AE:1167:UNK:O 12:AE:1171:UNK:CB 2.69 0.41 16:BA:329:VAL:HB 16:BA:342:GLN:HE21 1.85 0.41 16:BA:825:GLN:HA 16:BA:828:ILE:HD12 2.02 0.41 20:BE:503:ARG:NH1 20:BE:522:LEU:O 2.54 0.41 20:BE:592:ASP:HB3 20:BE:634:PHE:HD1 1.86 0.41 22:CB:1233:ASN:HD22 22:CB:1234:PHE:H 1.69 0.41 22:CB:855:LEU:HD13 22:CB:889:TYR:HD2 1.86 0.41 23:E1:36:LYS:HB3 23:E1:172:PRO:HA 2.02 0.41 31:R1:305:LYS:HA 31:R1:358:GLY:HA2 2.02 0.41 33:S1:364:UNK:O 33:S1:373:UNK:N 2.54 0.41 34:SA:1202:A:H1’ 34:SA:1207:C:H42 1.85 0.41 36:SF:112:HIS:NE2 36:SF:237:SER:O 2.54 0.41 40:SJ:103:GLN:HG3 40:SJ:164:ARG:HB3 2.02 0.41 43:SN:29:LYS:HG3 43:SN:100:TRP:CD1 2.55 0.41 2:3C:296:GLU:HG3 2:3C:298:ILE:HG12 2.03 0.41 5:3F:334:GLU:HG2 5:3F:351:ILE:HB 2.03 0.41 7:5A:589:U:H2’ 7:5A:590:G:C8 2.56 0.41 12:AE:775:ILE:O 12:AE:779:SER:OG 2.30 0.41 13:AF:141:UNK:O 13:AF:150:UNK:N 2.54 0.41 18:BC:281:THR:HG23 18:BC:327:ILE:HD13 2.02 0.41 18:BC:588:LYS:HG2 18:BC:589:GLN:HE21 1.85 0.41 20:BE:566:SER:HA 20:BE:581:LEU:O 2.21 0.41 21:CA:191:PHE:O 21:CA:195:GLU:HB2 2.21 0.41 24:E3:305:ARG:C 24:E3:307:VAL:N 2.74 0.41 31:R1:312:ARG:HH21 31:R1:347:ASN:HD21 1.69 0.41 34:SA:1173:C:O2’ 34:SA:1601:G:N2 2.51 0.41 34:SA:1759:C:H2’ 34:SA:1760:G:C8 2.56 0.41 34:SA:348:U:H5” 42:SM:106:ASN:HB2 2.02 0.41 34:SA:505:A:H2’ 34:SA:507:U:H5’ 2.02 0.41 38:SH:188:ARG:NH1 38:SH:191:ARG:CG 2.80 0.41 40:SJ:90:LEU:HD22 40:SJ:95:THR:HG21 2.02 0.41 46:SR:13:LYS:HG3 46:SR:14:LYS:H 1.85 0.41 48:SY:51:GLY:HA2 48:SY:77:ILE:HG13 2.03 0.41 1:3A:9:A:H2’ 1:3A:10:C:C6 2.56 0.41 1:3A:113:G:H1’ 1:3A:257:A:N6 2.35 0.41 3:3D:218:LYS:HZ1 3:3D:240:LEU:HD22 1.85 0.41 4:3E:174:ASP:O 4:3E:178:ASN:HB2 2.21 0.41 4:3E:314:ALA:HA 4:3E:317:ILE:HD12 2.02 0.41 12:AE:767:GLN:HA 12:AE:770:LYS:HB3 2.03 0.41 Continued on next page... Page 123 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 13:AF:407:UNK:O 13:AF:411:UNK:CB 2.69 0.41 14:AG:243:UNK:O 14:AG:251:UNK:N 2.53 0.41 14:AG:305:UNK:HA 14:AG:312:UNK:HA 2.02 0.41 15:B1:978:ARG:HG2 15:B1:980:LEU:H 1.85 0.41 16:BA:162:LEU:HD23 16:BA:199:PHE:HB2 2.02 0.41 16:BA:795:ASN:HA 16:BA:798:TRP:HD1 1.85 0.41 17:BB:214:ASP:HB2 17:BB:258:LYS:HD3 2.01 0.41 17:BB:37:GLY:HA3 17:BB:38:PRO:HD3 1.90 0.41 18:BC:256:ARG:NH1 18:BC:299:ASN:OD1 2.50 0.41 20:BE:462:PHE:O 20:BE:480:SER:N 2.54 0.41 22:CB:168:ILE:HD12 22:CB:171:ASN:H 1.86 0.41 22:CB:384:SER:HB3 22:CB:588:GLN:HE22 1.85 0.41 22:CB:748:LEU:HA 22:CB:787:GLU:OE2 2.21 0.41 34:SA:1049:U:H2’ 34:SA:1050:G:H8 1.86 0.41 34:SA:107:C:H2’ 34:SA:108:A:H8 1.85 0.41 34:SA:253:A:H2’ 34:SA:254:A:C8 2.56 0.41 34:SA:453:U:OP2 34:SA:455:C:N4 2.54 0.41 35:SC:161:ILE:HG22 35:SC:165:ARG:NH1 2.35 0.41 37:SG:130:ILE:H 37:SG:130:ILE:HG13 1.71 0.41 42:SM:55:ASP:OD2 42:SM:113:PRO:HD2 2.20 0.41 45:SP:31:THR:HG22 45:SP:38:THR:HA 2.03 0.41 5:3F:364:GLU:HG2 5:3F:366:GLN:H 1.85 0.41 12:AE:1477:UNK:O 12:AE:1481:UNK:CB 2.69 0.41 12:AE:1663:UNK:O 12:AE:1667:UNK:CB 2.69 0.41 15:B1:57:PRO:HD3 15:B1:101:ALA:HB3 2.02 0.41 16:BA:139:HIS:NE2 16:BA:176:ASP:OD1 2.50 0.41 17:BB:833:LEU:HD13 17:BB:836:ILE:HD13 2.03 0.41 19:BD:273:ARG:HB2 19:BD:284:LEU:HD11 2.03 0.41 19:BD:543:LEU:HD23 19:BD:556:PRO:HG3 2.03 0.41 21:CA:133:SER:O 21:CA:137:CYS:HB3 2.21 0.41 23:E2:104:ILE:HG23 23:E2:110:LEU:HD22 2.02 0.41 34:SA:1152:A:H2’ 34:SA:1153:G:C8 2.56 0.41 34:SA:877:G:H2’ 34:SA:878:G:H8 1.86 0.41 37:SG:26:ALA:HB2 46:SR:26:LYS:NZ 2.36 0.41 44:SO:124:ARG:HG2 44:SO:127:ARG:HH21 1.86 0.41 34:SA:576:G:O3’ 48:SY:63:GLN:NE2 2.54 0.41 49:SZ:55:VAL:HG22 49:SZ:75:VAL:HG23 2.02 0.41 57:U4:70:ASN:HA 57:U4:73:ASN:HD22 1.84 0.41 1:3A:252:C:H2’ 1:3A:253:G:C4 2.56 0.41 4:3E:288:THR:O 4:3E:292:GLY:HA2 2.21 0.41 4:3E:62:SER:HA 4:3E:67:LYS:HB3 2.03 0.41 Continued on next page... Page 124 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 12:AE:769:LEU:HB2 12:AE:773:GLN:HE22 1.85 0.41 17:BB:640:LYS:HE2 17:BB:652:LYS:HD2 2.02 0.41 18:BC:135:TYR:CD2 18:BC:137:THR:HG22 2.55 0.41 20:BE:797:ASP:N 20:BE:797:ASP:OD1 2.53 0.41 22:CB:563:ALA:O 22:CB:567:THR:OG1 2.24 0.41 28:MB:150:THR:HG21 28:MB:170:VAL:HG21 2.03 0.41 33:S1:159:UNK:O 33:S1:168:UNK:N 2.54 0.41 34:SA:1042:G:H2’ 34:SA:1043:A:H8 1.86 0.41 34:SA:1045:C:H2’ 34:SA:1046:G:H8 1.86 0.41 35:SC:91:VAL:HB 35:SC:96:LEU:HD13 2.03 0.41 40:SJ:39:GLY:O 40:SJ:59:ARG:HB3 2.21 0.41 46:SR:21:HIS:CE1 46:SR:68:ARG:NH1 2.89 0.41 56:U3:395:UNK:HA 56:U3:440:UNK:HA 2.03 0.41 1:3A:66:U:H2’ 1:3A:67:G:C8 2.55 0.40 3:3D:44:PHE:CE2 3:3D:46:LYS:HB3 2.56 0.40 7:5A:244:U:H2’ 7:5A:245:C:C6 2.56 0.40 10:AC:470:UNK:O 10:AC:474:UNK:CB 2.68 0.40 12:AE:756:LEU:O 12:AE:760:PHE:CB 2.65 0.40 15:B1:216:TRP:O 15:B1:220:HIS:ND1 2.44 0.40 18:BC:150:LEU:HD12 18:BC:159:LYS:HG2 2.02 0.40 18:BC:264:ASP:N 18:BC:264:ASP:OD1 2.47 0.40 19:BD:403:VAL:HG12 20:BE:605:LEU:HG 2.03 0.40 19:BD:424:ILE:O 19:BD:432:VAL:HA 2.21 0.40 22:CB:333:ASN:O 22:CB:337:LEU:CB 2.70 0.40 23:E1:129:ARG:HB3 34:SA:1192:C:H5” 2.02 0.40 23:E2:143:GLN:O 23:E2:147:LYS:HB2 2.20 0.40 23:E2:36:LYS:HD2 23:E2:172:PRO:HA 2.02 0.40 24:E3:300:ASN:HB3 24:E3:303:ILE:HG12 2.02 0.40 24:E3:334:GLU:O 24:E3:338:ALA:CB 2.60 0.40 28:MB:112:PRO:HD3 28:MB:184:GLU:HG2 2.03 0.40 32:R2:1638:ARG:NH2 32:R2:1662:GLU:OE1 2.52 0.40 34:SA:1164:G:OP1 37:SG:166:ARG:NH2 2.55 0.40 34:SA:1224:A:H2’ 34:SA:1225:U:H6 1.86 0.40 34:SA:1727:G:H2’ 34:SA:1728:A:C8 2.55 0.40 34:SA:289:U:H2’ 34:SA:290:G:H8 1.86 0.40 49:SZ:54:ALA:O 49:SZ:76:TYR:N 2.43 0.40 2:3B:255:LEU:O 2:3B:259:MET:HB2 2.21 0.40 4:3E:234:ARG:O 4:3E:238:ALA:CB 2.69 0.40 4:3E:299:LEU:HD23 4:3E:341:LEU:HD12 2.03 0.40 5:3F:157:LEU:HA 5:3F:191:SER:HA 2.03 0.40 5:3F:230:ASN:HD21 5:3F:258:ARG:HG3 1.86 0.40 Continued on next page... Page 125 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 5:3F:323:ASP:OD2 5:3F:342:ARG:HB2 2.21 0.40 9:AB:4:UNK:HA 9:AB:284:UNK:HA 2.04 0.40 12:AE:566:CYS:O 12:AE:570:ALA:CB 2.68 0.40 12:AE:628:ASN:O 12:AE:632:THR:OG1 2.26 0.40 14:AG:446:UNK:O 14:AG:454:UNK:N 2.55 0.40 16:BA:159:ARG:HA 16:BA:175:VAL:HB 2.03 0.40 17:BB:592:SER:HB3 17:BB:598:LYS:HZ1 1.87 0.40 17:BB:821:LEU:HB3 17:BB:863:PHE:HE2 1.87 0.40 18:BC:50:ARG:HB2 18:BC:375:ALA:HB2 2.03 0.40 19:BD:411:TYR:CE1 19:BD:423:LEU:O 2.74 0.40 18:BC:239:VAL:HB 22:CB:636:GLU:HG3 2.03 0.40 22:CB:738:TYR:O 22:CB:742:PHE:HB3 2.22 0.40 33:S1:157:UNK:N 33:S1:170:UNK:O 2.54 0.40 34:SA:1228:G:H21 43:SN:66:VAL:HG22 1.86 0.40 54:U1:14:UNK:HA 54:U1:30:UNK:HA 2.02 0.40 17:BB:405:LEU:HD22 17:BB:459:LEU:HD21 2.03 0.40 18:BC:474:SER:H 18:BC:478:GLN:NE2 2.20 0.40 19:BD:288:LEU:HD22 19:BD:324:TRP:CD1 2.56 0.40 19:BD:424:ILE:HD13 19:BD:433:TRP:CD1 2.56 0.40 20:BE:192:ASN:O 20:BE:196:GLY:N 2.54 0.40 20:BE:440:ALA:HB3 20:BE:455:PHE:HB2 2.03 0.40 22:CB:267:ILE:HG21 22:CB:295:LEU:HG 2.03 0.40 34:SA:116:U:H2’ 34:SA:117:U:C6 2.57 0.40 34:SA:190:C:H1’ 34:SA:191:C:H5’ 2.03 0.40 34:SA:200:A:H2’ 34:SA:201:G:C8 2.56 0.40 34:SA:976:G:H2’ 34:SA:977:A:C8 2.56 0.40 39:SI:50:ASP:HA 39:SI:56:LYS:HA 2.02 0.40 41:SK:171:ARG:HH21 41:SK:175:ARG:NH2 2.19 0.40 7:5A:525:U:H2’ 7:5A:526:U:C6 2.57 0.40 14:AG:239:UNK:HA 14:AG:260:UNK:HA 2.04 0.40 17:BB:496:THR:OG1 17:BB:520:LEU:O 2.31 0.40 18:BC:222:LEU:HB2 18:BC:235:LYS:H 1.86 0.40 19:BD:412:GLN:NE2 19:BD:459:GLN:OE1 2.46 0.40 20:BE:97:LYS:HZ3 20:BE:111:GLU:CD 2.23 0.40 22:CB:227:LYS:O 22:CB:231:TYR:HB2 2.20 0.40 22:CB:853:ARG:HH21 22:CB:889:TYR:HE1 1.69 0.40 24:E3:258:VAL:O 24:E3:262:ALA:HB3 2.21 0.40 24:E3:300:ASN:HD22 24:E3:301:TYR:H 1.70 0.40 24:E3:318:PHE:HE2 24:E3:351:HIS:HB3 1.86 0.40 24:E3:398:ARG:HB3 24:E3:443:THR:HA 2.03 0.40 26:K1:166:VAL:HA 26:K1:171:VAL:HA 2.04 0.40 Continued on next page... Page 126 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Interatomic Clash Atom-1 Atom-2 distance (Å) overlap (Å) 34:SA:356:G:H2’ 34:SA:357:G:C8 2.55 0.40 34:SA:922:G:H2’ 34:SA:923:A:C8 2.56 0.40 34:SA:936:G:OP1 34:SA:1074:G:N2 2.45 0.40 34:SA:96:G:N1 34:SA:403:G:OP1 2.55 0.40 37:SG:21:THR:O 37:SG:21:THR:OG1 2.37 0.40 48:SY:95:PHE:O 48:SY:142:LYS:NZ 2.48 0.40 3:3D:233:SER:HA 3:3D:234:LEU:HA 1.92 0.40 6:3G:84:ARG:HG3 6:3G:96:PRO:HB3 2.03 0.40 7:5A:291:G:H2’ 7:5A:292:A:H8 1.86 0.40 12:AE:180:ILE:HD12 12:AE:223:PHE:HZ 1.86 0.40 15:B1:53:VAL:HG13 15:B1:118:LEU:HD22 2.02 0.40 19:BD:321:MET:HG3 19:BD:342:PHE:HD2 1.86 0.40 19:BD:411:TYR:CG 19:BD:412:GLN:N 2.90 0.40 34:SA:921:U:H2’ 34:SA:922:G:C8 2.57 0.40 36:SF:182:TYR:CZ 36:SF:190:GLY:HA2 2.57 0.40 47:SX:17:ALA:HA 47:SX:22:LYS:HZ3 1.86 0.40

There are no symmetry-related clashes.

5.3 Torsion angles ○i

5.3.1 Protein backbone ○i

In the following table, the Percentiles column shows the percent Ramachandran outliers of the chain as a percentile score with respect to all PDB entries followed by that with respect to all EM entries. The Analysed column shows the number of residues for which the backbone conformation was analysed, and the total number of residues.

Mol Chain Analysed Favoured Allowed Outliers Percentiles 2 3B 237/327 (72%) 227 (96%) 10 (4%) 0 100 100 2 3C 237/327 (72%) 216 (91%) 21 (9%) 0 100 100 3 3D 366/504 (73%) 343 (94%) 23 (6%) 0 100 100 4 3E 378/511 (74%) 358 (95%) 20 (5%) 0 100 100 5 3F 353/573 (62%) 334 (95%) 19 (5%) 0 100 100 6 3G 120/126 (95%) 114 (95%) 6 (5%) 0 100 100 6 3H 120/126 (95%) 118 (98%) 2 (2%) 0 100 100 Continued on next page... Page 127 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Analysed Favoured Allowed Outliers Percentiles 12 AE 751/1769 (42%) 703 (94%) 48 (6%) 0 100 100 15 B1 528/1183 (45%) 481 (91%) 47 (9%) 0 100 100 16 BA 747/923 (81%) 669 (90%) 76 (10%) 2 (0%) 41 77 17 BB 770/943 (82%) 682 (89%) 86 (11%) 2 (0%) 41 77 18 BC 779/817 (95%) 678 (87%) 96 (12%) 5 (1%) 25 66 19 BD 317/594 (53%) 278 (88%) 39 (12%) 0 100 100 20 BE 741/939 (79%) 699 (94%) 42 (6%) 0 100 100 21 CA 190/297 (64%) 180 (95%) 10 (5%) 0 100 100 22 CB 1086/1237 (88%) 1059 (98%) 27 (2%) 0 100 100 23 E1 213/252 (84%) 209 (98%) 4 (2%) 0 100 100 23 E2 210/252 (83%) 204 (97%) 6 (3%) 0 100 100 24 E3 256/483 (53%) 242 (94%) 12 (5%) 2 (1%) 19 60 26 K1 173/316 (55%) 167 (96%) 6 (4%) 0 100 100 27 MA 131/183 (72%) 128 (98%) 3 (2%) 0 100 100 28 MB 182/290 (63%) 168 (92%) 14 (8%) 0 100 100 29 MC 24/593 (4%) 24 (100%) 0 0 100 100 30 P1 171/274 (62%) 161 (94%) 10 (6%) 0 100 100 31 R1 353/367 (96%) 348 (99%) 5 (1%) 0 100 100 32 R2 266/1729 (15%) 262 (98%) 4 (2%) 0 100 100 35 SC 212/255 (83%) 170 (80%) 37 (18%) 5 (2%) 6 33 36 SF 235/261 (90%) 210 (89%) 24 (10%) 1 (0%) 34 72 37 SG 204/225 (91%) 181 (89%) 21 (10%) 2 (1%) 15 55 38 SH 170/236 (72%) 159 (94%) 11 (6%) 0 100 100 39 SI 161/190 (85%) 142 (88%) 18 (11%) 1 (1%) 25 66 40 SJ 166/200 (83%) 146 (88%) 19 (11%) 1 (1%) 25 66 41 SK 173/197 (88%) 157 (91%) 15 (9%) 1 (1%) 25 66 42 SM 139/156 (89%) 124 (89%) 13 (9%) 2 (1%) 11 46 43 SN 122/143 (85%) 83 (68%) 35 (29%) 4 (3%) 4 26 44 SO 132/151 (87%) 129 (98%) 3 (2%) 0 100 100 45 SP 107/137 (78%) 95 (89%) 12 (11%) 0 100 100 46 SR 123/143 (86%) 113 (92%) 8 (6%) 2 (2%) 9 44 Continued on next page... Page 128 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Analysed Favoured Allowed Outliers Percentiles 47 SX 127/130 (98%) 115 (91%) 12 (9%) 0 100 100 48 SY 101/145 (70%) 82 (81%) 16 (16%) 3 (3%) 4 28 49 SZ 99/135 (73%) 88 (89%) 11 (11%) 0 100 100 50 Sc 77/82 (94%) 67 (87%) 10 (13%) 0 100 100 51 Sd 61/67 (91%) 57 (93%) 4 (7%) 0 100 100 52 Sf 28/63 (44%) 28 (100%) 0 0 100 100 53 Sg 49/152 (32%) 37 (76%) 12 (24%) 0 100 100 57 U4 124/189 (66%) 115 (93%) 8 (6%) 1 (1%) 19 60 58 U5 246/274 (90%) 229 (93%) 17 (7%) 0 100 100 All All 12555/19466 (64%) 11579 (92%) 942 (8%) 34 (0%) 44 77

All (34) Ramachandran outliers are listed below:

Mol Chain Res Type 18 BC 298 SER 24 E3 306 ALA 35 SC 207 LEU 35 SC 209 ASN 37 SG 50 GLU 37 SG 51 VAL 43 SN 130 THR 46 SR 59 LYS 17 BB 798 ASN 18 BC 137 THR 24 E3 206 LEU 35 SC 181 LEU 35 SC 206 PRO 36 SF 195 ILE 42 SM 4 GLU 43 SN 131 ASP 48 SY 97 ASP 16 BA 130 ASP 18 BC 297 LEU 42 SM 3 THR 43 SN 90 LYS 16 BA 556 ARG 17 BB 797 VAL 18 BC 90 LEU 18 BC 772 LEU Continued on next page... Page 129 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 40 SJ 23 LYS 43 SN 126 TRP 48 SY 70 LYS 35 SC 210 ILE 39 SI 131 PHE 41 SK 134 ILE 46 SR 33 GLY 48 SY 96 VAL 57 U4 175 VAL

5.3.2 Protein sidechains ○i

In the following table, the Percentiles column shows the percent sidechain outliers of the chain as a percentile score with respect to all PDB entries followed by that with respect to all EM entries. The Analysed column shows the number of residues for which the sidechain conformation was analysed, and the total number of residues.

Mol Chain Analysed Rotameric Outliers Percentiles 2 3B 202/240 (84%) 200 (99%) 2 (1%) 76 86 2 3C 202/240 (84%) 202 (100%) 0 100 100 3 3D 317/435 (73%) 315 (99%) 2 (1%) 86 92 4 3E 314/433 (72%) 312 (99%) 2 (1%) 86 92 5 3F 317/503 (63%) 316 (100%) 1 (0%) 92 95 6 3G 101/104 (97%) 100 (99%) 1 (1%) 76 86 6 3H 101/104 (97%) 100 (99%) 1 (1%) 76 86 12 AE 698/744 (94%) 688 (99%) 10 (1%) 67 80 15 B1 471/1039 (45%) 466 (99%) 5 (1%) 73 84 16 BA 663/812 (82%) 653 (98%) 10 (2%) 65 80 17 BB 687/832 (83%) 676 (98%) 11 (2%) 62 79 18 BC 687/719 (96%) 683 (99%) 4 (1%) 86 92 19 BD 281/529 (53%) 277 (99%) 4 (1%) 67 80 20 BE 657/819 (80%) 654 (100%) 3 (0%) 88 93 21 CA 178/274 (65%) 177 (99%) 1 (1%) 86 92 22 CB 1000/1125 (89%) 994 (99%) 6 (1%) 86 92 23 E1 191/222 (86%) 191 (100%) 0 100 100 Continued on next page... Page 130 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Analysed Rotameric Outliers Percentiles 23 E2 193/222 (87%) 193 (100%) 0 100 100 24 E3 232/424 (55%) 228 (98%) 4 (2%) 60 78 26 K1 158/289 (55%) 158 (100%) 0 100 100 27 MA 123/172 (72%) 121 (98%) 2 (2%) 62 79 28 MB 164/258 (64%) 164 (100%) 0 100 100 29 MC 24/148 (16%) 24 (100%) 0 100 100 30 P1 151/238 (63%) 150 (99%) 1 (1%) 84 90 31 R1 302/312 (97%) 301 (100%) 1 (0%) 92 95 32 R2 235/1544 (15%) 233 (99%) 2 (1%) 78 87 35 SC 191/224 (85%) 191 (100%) 0 100 100 36 SF 203/222 (91%) 199 (98%) 4 (2%) 55 74 37 SG 173/191 (91%) 171 (99%) 2 (1%) 71 83 38 SH 141/201 (70%) 138 (98%) 3 (2%) 53 72 39 SI 146/170 (86%) 146 (100%) 0 100 100 40 SJ 138/161 (86%) 137 (99%) 1 (1%) 84 90 41 SK 149/166 (90%) 145 (97%) 4 (3%) 44 65 42 SM 128/137 (93%) 123 (96%) 5 (4%) 32 56 43 SN 88/119 (74%) 85 (97%) 3 (3%) 37 60 44 SO 117/128 (91%) 115 (98%) 2 (2%) 60 78 45 SP 66/105 (63%) 66 (100%) 0 100 100 46 SR 105/119 (88%) 103 (98%) 2 (2%) 57 75 47 SX 110/111 (99%) 110 (100%) 0 100 100 48 SY 85/120 (71%) 84 (99%) 1 (1%) 71 83 49 SZ 85/113 (75%) 83 (98%) 2 (2%) 49 69 50 Sc 68/71 (96%) 68 (100%) 0 100 100 51 Sd 56/60 (93%) 53 (95%) 3 (5%) 22 47 52 Sf 27/54 (50%) 27 (100%) 0 100 100 53 Sg 43/135 (32%) 42 (98%) 1 (2%) 50 70 57 U4 110/169 (65%) 110 (100%) 0 100 100 58 U5 231/256 (90%) 229 (99%) 2 (1%) 78 87 All All 11109/15813 (70%) 11001 (99%) 108 (1%) 77 86 Page 131 Full wwPDB EM Validation Report EMD-6695, 5WYJ

All (108) residues with a non-rotameric sidechain are listed below:

Mol Chain Res Type 2 3B 145 ASN 2 3B 231 ARG 3 3D 285 ARG 3 3D 391 ASN 4 3E 50 PHE 4 3E 178 ASN 5 3F 555 ASN 6 3G 105 ASN 6 3H 105 ASN 12 AE 83 ARG 12 AE 134 MET 12 AE 232 ASN 12 AE 487 THR 12 AE 559 ASN 12 AE 615 ASN 12 AE 630 TYR 12 AE 648 LYS 12 AE 729 LYS 12 AE 781 ASN 15 B1 246 ARG 15 B1 812 MET 15 B1 831 ARG 15 B1 833 ARG 15 B1 853 ARG 16 BA 78 ARG 16 BA 94 ASN 16 BA 306 ASN 16 BA 418 ARG 16 BA 420 ARG 16 BA 434 ASN 16 BA 619 ARG 16 BA 777 ARG 16 BA 811 ASN 16 BA 837 ASN 17 BB 20 ASN 17 BB 22 ASN 17 BB 118 ASN 17 BB 271 PHE 17 BB 411 ASN 17 BB 587 MET 17 BB 596 ASN 17 BB 620 ASN Continued on next page... Page 132 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 17 BB 629 ASN 17 BB 770 ASN 17 BB 799 LYS 18 BC 58 ASN 18 BC 157 ASN 18 BC 227 MET 18 BC 487 ARG 19 BD 357 ASN 19 BD 369 ASN 19 BD 440 ASN 19 BD 446 ARG 20 BE 392 ARG 20 BE 449 ARG 20 BE 587 ARG 21 CA 46 ASN 22 CB 155 ASN 22 CB 211 LYS 22 CB 216 LYS 22 CB 261 ASN 22 CB 683 ASN 22 CB 1233 ASN 24 E3 206 LEU 24 E3 300 ASN 24 E3 304 TYR 24 E3 457 ARG 27 MA 48 ASN 27 MA 71 ARG 30 P1 142 ASN 31 R1 301 MET 32 R2 1466 ARG 32 R2 1615 ARG 36 SF 39 ARG 36 SF 59 ARG 36 SF 198 LYS 36 SF 240 LYS 37 SG 122 ASN 37 SG 157 ARG 38 SH 25 ARG 38 SH 68 LEU 38 SH 188 ARG 40 SJ 64 ASN 41 SK 99 LEU Continued on next page... Page 133 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 41 SK 120 LYS 41 SK 138 LYS 41 SK 149 ARG 42 SM 5 LEU 42 SM 67 ARG 42 SM 116 ARG 42 SM 129 ARG 42 SM 141 LYS 43 SN 43 ARG 43 SN 91 VAL 43 SN 125 ASN 44 SO 55 ARG 44 SO 64 ARG 46 SR 23 LYS 46 SR 106 LYS 48 SY 144 ARG 49 SZ 32 ARG 49 SZ 34 ASN 51 Sd 14 LYS 51 Sd 49 ARG 51 Sd 64 ARG 53 Sg 123 ASN 58 U5 68 ARG 58 U5 105 ASN

Sometimes sidechains can be flipped to improve hydrogen bonding and reduce clashes. All (149) such sidechains are listed below: Mol Chain Res Type 2 3B 91 HIS 2 3B 145 ASN 3 3D 23 GLN 3 3D 37 GLN 3 3D 67 ASN 3 3D 125 GLN 3 3D 151 GLN 3 3D 176 GLN 3 3D 230 ASN 3 3D 318 HIS 3 3D 391 ASN 4 3E 61 ASN 4 3E 178 ASN 4 3E 256 ASN Continued on next page... Page 134 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 5 3F 195 GLN 5 3F 230 ASN 5 3F 555 ASN 6 3G 105 ASN 6 3H 105 ASN 12 AE 29 HIS 12 AE 43 GLN 12 AE 219 ASN 12 AE 227 ASN 12 AE 232 ASN 12 AE 258 HIS 12 AE 480 ASN 12 AE 615 ASN 12 AE 781 ASN 15 B1 136 ASN 15 B1 218 ASN 15 B1 233 HIS 15 B1 548 ASN 15 B1 893 ASN 15 B1 904 ASN 15 B1 909 ASN 16 BA 9 ASN 16 BA 17 GLN 16 BA 92 HIS 16 BA 94 ASN 16 BA 128 ASN 16 BA 306 ASN 16 BA 434 ASN 16 BA 483 GLN 16 BA 538 GLN 16 BA 552 ASN 16 BA 608 ASN 16 BA 811 ASN 16 BA 837 ASN 17 BB 20 ASN 17 BB 22 ASN 17 BB 118 ASN 17 BB 360 ASN 17 BB 390 GLN 17 BB 411 ASN 17 BB 596 ASN 17 BB 620 ASN Continued on next page... Page 135 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 17 BB 628 HIS 17 BB 629 ASN 17 BB 656 HIS 17 BB 770 ASN 18 BC 28 ASN 18 BC 58 ASN 18 BC 83 GLN 18 BC 184 HIS 18 BC 478 GLN 18 BC 576 ASN 18 BC 585 ASN 18 BC 587 GLN 18 BC 589 GLN 18 BC 667 GLN 19 BD 282 ASN 19 BD 335 GLN 19 BD 357 ASN 19 BD 369 ASN 19 BD 440 ASN 19 BD 498 ASN 19 BD 520 ASN 20 BE 300 GLN 20 BE 473 ASN 20 BE 900 ASN 20 BE 903 GLN 21 CA 35 HIS 21 CA 46 ASN 22 CB 120 HIS 22 CB 155 ASN 22 CB 261 ASN 22 CB 333 ASN 22 CB 409 ASN 22 CB 436 HIS 22 CB 441 GLN 22 CB 445 ASN 22 CB 560 ASN 22 CB 582 GLN 22 CB 588 GLN 22 CB 683 ASN 22 CB 904 HIS 22 CB 1029 ASN 22 CB 1078 HIS Continued on next page... Page 136 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 22 CB 1154 ASN 22 CB 1233 ASN 23 E1 111 GLN 23 E2 115 GLN 24 E3 250 ASN 24 E3 269 ASN 24 E3 300 ASN 24 E3 351 HIS 24 E3 449 HIS 26 K1 167 GLN 27 MA 48 ASN 28 MB 226 ASN 28 MB 236 HIS 30 P1 142 ASN 31 R1 248 GLN 32 R2 1416 GLN 32 R2 1616 ASN 32 R2 1661 GLN 32 R2 1693 ASN 35 SC 49 ASN 35 SC 118 GLN 35 SC 146 GLN 35 SC 149 GLN 35 SC 194 ASN 35 SC 199 ASN 35 SC 208 GLN 36 SF 98 ASN 37 SG 95 ASN 37 SG 100 ASN 37 SG 131 GLN 37 SG 158 GLN 38 SH 185 GLN 39 SI 147 ASN 40 SJ 64 ASN 42 SM 18 HIS 43 SN 125 ASN 44 SO 62 GLN 45 SP 80 HIS 46 SR 21 HIS 47 SX 15 ASN 47 SX 42 GLN 47 SX 80 ASN Continued on next page... Page 137 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 47 SX 92 ASN 48 SY 63 GLN 48 SY 79 ASN 57 U4 57 GLN 57 U4 73 ASN 58 U5 41 ASN 58 U5 105 ASN 58 U5 217 GLN 58 U5 248 ASN

5.3.3 RNA ○i

Mol Chain Analysed Backbone Outliers Pucker Outliers 1 3A 151/333 (45%) 31 (20%) 1 (0%) 34 SA 1091/1812 (60%) 268 (24%) 22 (2%) 7 5A 452/700 (64%) 102 (22%) 4 (0%) All All 1694/2845 (59%) 401 (23%) 27 (1%)

All (401) RNA backbone outliers are listed below:

Mol Chain Res Type 1 3A 9 A 1 3A 14 A 1 3A 15 U 1 3A 22 A 1 3A 24 U 1 3A 55 A 1 3A 58 A 1 3A 61 G 1 3A 64 A 1 3A 65 C 1 3A 79 G 1 3A 106 C 1 3A 109 G 1 3A 111 G 1 3A 114 A 1 3A 207 A 1 3A 254 A 1 3A 255 U 1 3A 256 G 1 3A 261 U Continued on next page... Page 138 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 1 3A 262 G 1 3A 267 A 1 3A 317 A 1 3A 318 U 1 3A 319 G 1 3A 322 A 1 3A 323 G 1 3A 324 U 1 3A 325 C 1 3A 328 A 1 3A 329 C 7 5A 12 G 7 5A 13 U 7 5A 14 U 7 5A 27 G 7 5A 58 U 7 5A 59 U 7 5A 60 G 7 5A 63 G 7 5A 67 G 7 5A 68 U 7 5A 69 U 7 5A 70 A 7 5A 76 U 7 5A 91 U 7 5A 101 G 7 5A 103 G 7 5A 106 A 7 5A 109 C 7 5A 121 G 7 5A 123 C 7 5A 124 A 7 5A 125 G 7 5A 129 U 7 5A 130 G 7 5A 142 U 7 5A 143 A 7 5A 145 A 7 5A 152 U 7 5A 153 U 7 5A 158 G 7 5A 169 A Continued on next page... Page 139 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 7 5A 170 U 7 5A 171 G 7 5A 176 U 7 5A 178 G 7 5A 181 A 7 5A 200 A 7 5A 211 G 7 5A 225 U 7 5A 226 U 7 5A 227 U 7 5A 234 A 7 5A 236 C 7 5A 237 A 7 5A 238 G 7 5A 239 U 7 5A 240 C 7 5A 246 G 7 5A 253 U 7 5A 261 U 7 5A 266 U 7 5A 267 U 7 5A 278 G 7 5A 280 A 7 5A 294 U 7 5A 338 A 7 5A 340 U 7 5A 349 G 7 5A 353 A 7 5A 354 G 7 5A 355 C 7 5A 361 G 7 5A 362 C 7 5A 363 A 7 5A 368 U 7 5A 370 U 7 5A 371 G 7 5A 372 A 7 5A 382 U 7 5A 383 G 7 5A 384 U 7 5A 386 A 7 5A 387 C Continued on next page... Page 140 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 7 5A 388 C 7 5A 391 C 7 5A 392 U 7 5A 393 C 7 5A 394 U 7 5A 395 C 7 5A 401 A 7 5A 402 G 7 5A 403 C 7 5A 404 G 7 5A 405 A 7 5A 419 A 7 5A 433 C 7 5A 434 G 7 5A 435 G 7 5A 436 G 7 5A 441 C 7 5A 442 U 7 5A 451 G 7 5A 452 A 7 5A 459 U 7 5A 464 G 7 5A 503 C 7 5A 508 C 7 5A 511 G 7 5A 514 U 7 5A 527 U 7 5A 528 G 7 5A 534 A 34 SA 8 U 34 SA 10 G 34 SA 25 C 34 SA 26 A 34 SA 27 U 34 SA 34 G 34 SA 39 A 34 SA 47 A 34 SA 50 C 34 SA 93 A 34 SA 94 U 34 SA 95 G 34 SA 103 A Continued on next page... Page 141 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 114 C 34 SA 116 U 34 SA 127 G 34 SA 128 U 34 SA 129 U 34 SA 132 U 34 SA 133 U 34 SA 134 U 34 SA 135 A 34 SA 137 U 34 SA 138 A 34 SA 139 C 34 SA 140 A 34 SA 145 A 34 SA 146 U 34 SA 152 U 34 SA 153 G 34 SA 156 A 34 SA 159 U 34 SA 162 A 34 SA 179 A 34 SA 180 A 34 SA 181 A 34 SA 185 U 34 SA 186 C 34 SA 188 A 34 SA 190 C 34 SA 191 C 34 SA 192 U 34 SA 193 U 34 SA 194 U 34 SA 195 G 34 SA 196 G 34 SA 199 G 34 SA 200 A 34 SA 204 G 34 SA 215 A 34 SA 251 A 34 SA 261 U 34 SA 265 A 34 SA 266 A 34 SA 270 C Continued on next page... Page 142 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 272 U 34 SA 273 G 34 SA 276 C 34 SA 277 U 34 SA 278 U 34 SA 279 G 34 SA 280 U 34 SA 281 G 34 SA 288 A 34 SA 299 A 34 SA 302 U 34 SA 316 A 34 SA 321 C 34 SA 322 G 34 SA 337 G 34 SA 338 C 34 SA 341 A 34 SA 348 U 34 SA 351 C 34 SA 352 A 34 SA 359 A 34 SA 360 A 34 SA 361 C 34 SA 369 A 34 SA 370 A 34 SA 372 G 34 SA 378 A 34 SA 379 U 34 SA 380 U 34 SA 381 C 34 SA 387 A 34 SA 399 A 34 SA 400 A 34 SA 401 A 34 SA 402 C 34 SA 403 G 34 SA 404 G 34 SA 416 A 34 SA 417 A 34 SA 418 G 34 SA 428 A 34 SA 433 C Continued on next page... Page 143 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 440 U 34 SA 444 C 34 SA 452 A 34 SA 459 G 34 SA 466 U 34 SA 467 G 34 SA 468 A 34 SA 470 A 34 SA 475 A 34 SA 477 A 34 SA 484 C 34 SA 485 A 34 SA 486 G 34 SA 487 G 34 SA 488 G 34 SA 493 U 34 SA 494 U 34 SA 501 U 34 SA 503 G 34 SA 504 U 34 SA 506 A 34 SA 507 U 34 SA 508 U 34 SA 510 G 34 SA 511 A 34 SA 515 A 34 SA 522 U 34 SA 525 A 34 SA 526 A 34 SA 527 A 34 SA 528 U 34 SA 532 U 34 SA 538 A 34 SA 539 G 34 SA 540 G 34 SA 542 A 34 SA 543 C 34 SA 545 A 34 SA 546 U 34 SA 548 G 34 SA 558 U 34 SA 559 C Continued on next page... Page 144 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 561 G 34 SA 570 A 34 SA 572 C 34 SA 576 G 34 SA 585 A 34 SA 592 A 34 SA 594 A 34 SA 605 A 34 SA 635 A 34 SA 862 A 34 SA 863 A 34 SA 865 A 34 SA 873 U 34 SA 876 G 34 SA 898 A 34 SA 914 G 34 SA 928 U 34 SA 929 A 34 SA 930 A 34 SA 932 U 34 SA 933 A 34 SA 935 U 34 SA 942 G 34 SA 951 A 34 SA 960 U 34 SA 966 A 34 SA 978 A 34 SA 1040 G 34 SA 1053 G 34 SA 1058 U 34 SA 1059 U 34 SA 1060 U 34 SA 1061 A 34 SA 1063 U 34 SA 1072 C 34 SA 1082 C 34 SA 1083 G 34 SA 1084 A 34 SA 1086 A 34 SA 1087 A 34 SA 1150 G 34 SA 1151 A Continued on next page... Page 145 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 1155 G 34 SA 1156 C 34 SA 1157 A 34 SA 1158 C 34 SA 1159 C 34 SA 1160 A 34 SA 1164 G 34 SA 1165 G 34 SA 1167 G 34 SA 1193 A 34 SA 1207 C 34 SA 1209 C 34 SA 1212 G 34 SA 1217 A 34 SA 1218 G 34 SA 1227 A 34 SA 1228 G 34 SA 1229 G 34 SA 1238 A 34 SA 1243 G 34 SA 1244 A 34 SA 1245 G 34 SA 1246 C 34 SA 1251 U 34 SA 1256 A 34 SA 1265 G 34 SA 1269 U 34 SA 1270 G 34 SA 1272 U 34 SA 1273 G 34 SA 1275 A 34 SA 1276 U 34 SA 1278 G 34 SA 1282 U 34 SA 1427 A 34 SA 1428 G 34 SA 1431 C 34 SA 1432 U 34 SA 1433 G 34 SA 1435 G 34 SA 1436 A 34 SA 1437 U Continued on next page... Page 146 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 1440 C 34 SA 1441 C 34 SA 1442 U 34 SA 1444 A 34 SA 1446 A 34 SA 1448 G 34 SA 1450 U 34 SA 1453 G 34 SA 1461 C 34 SA 1471 A 34 SA 1474 G 34 SA 1475 A 34 SA 1482 C 34 SA 1485 C 34 SA 1487 A 34 SA 1496 U 34 SA 1500 C 34 SA 1506 G 34 SA 1535 U 34 SA 1536 G 34 SA 1537 C 34 SA 1570 A 34 SA 1572 G 34 SA 1574 G 34 SA 1582 U 34 SA 1584 G 34 SA 1599 C 34 SA 1600 A 34 SA 1616 G 34 SA 1617 U 34 SA 1631 A 34 SA 1657 U 34 SA 1658 G 34 SA 1684 U 34 SA 1686 C 34 SA 1716 C 34 SA 1717 G 34 SA 1754 A 34 SA 1756 A 34 SA 1758 U 34 SA 1767 G 34 SA 1796 C Continued on next page... Page 147 Full wwPDB EM Validation Report EMD-6695, 5WYJ

Continued from previous page... Mol Chain Res Type 34 SA 1804 A 34 SA 1806 A 34 SA 1812 U

All (27) RNA pucker outliers are listed below:

Mol Chain Res Type 1 3A 78 G 7 5A 382 U 7 5A 385 A 7 5A 526 U 7 5A 527 U 34 SA 25 C 34 SA 139 C 34 SA 158 U 34 SA 192 U 34 SA 278 U 34 SA 351 C 34 SA 417 A 34 SA 467 G 34 SA 487 G 34 SA 503 G 34 SA 591 A 34 SA 913 G 34 SA 929 A 34 SA 1083 G 34 SA 1157 A 34 SA 1216 C 34 SA 1226 A 34 SA 1244 A 34 SA 1250 U 34 SA 1441 C 34 SA 1481 C 34 SA 1573 A

5.4 Non-standard residues in protein, DNA, RNA chains ○i

There are no non-standard protein/DNA/RNA residues in this entry. Page 148 Full wwPDB EM Validation Report EMD-6695, 5WYJ

5.5 Carbohydrates ○i

There are no monosaccharides in this entry.

5.6 Ligand geometry ○i

There are no ligands in this entry.

5.7 Other polymers ○i

There are no such residues in this entry.

5.8 Polymer linkage issues ○i

There are no chain breaks in this entry. Page 149 Full wwPDB EM Validation Report EMD-6695, 5WYJ

6 Map visualisation ○i

This section contains visualisations of the EMDB entry EMD-6695. These allow visual inspection of the internal detail of the map and identification of artifacts.

No raw map or half-maps were deposited for this entry and therefore no images, graphs, etc. pertaining to the raw map can be shown.

6.1 Orthogonal projections ○i

6.1.1 Primary map

X Y Z

The images above show the map projected in three orthogonal directions.

6.2 Central slices ○i

6.2.1 Primary map

X Index: 200 Y Index: 200 Z Index: 200 Page 150 Full wwPDB EM Validation Report EMD-6695, 5WYJ

The images above show central slices of the map in three orthogonal directions.

6.3 Largest variance slices ○i

6.3.1 Primary map

X Index: 221 Y Index: 178 Z Index: 187

The images above show the largest variance slices of the map in three orthogonal directions.

6.4 Orthogonal surface views ○i

6.4.1 Primary map

X Y Z

The images above show the 3D surface view of the map at the recommended contour level 0.09. These images, in conjunction with the slice images, may facilitate assessment of whether an ap- propriate contour level has been provided. Page 151 Full wwPDB EM Validation Report EMD-6695, 5WYJ

6.5 Mask visualisation ○i

This section was not generated. No masks/segmentation were deposited. Page 152 Full wwPDB EM Validation Report EMD-6695, 5WYJ

7 Map analysis ○i

This section contains the results of statistical analysis of the map.

7.1 Map-value distribution ○i

The map-value distribution is plotted in 128 intervals along the x-axis. The y-axis is logarithmic. A spike in this graph at zero usually indicates that the volume has been masked. Page 153 Full wwPDB EM Validation Report EMD-6695, 5WYJ

7.2 Volume estimate ○i

The volume at the recommended contour level is 1973 nm3; this corresponds to an approximate mass of 1782 kDa. The volume estimate graph shows how the enclosed volume varies with the contour level. The recommended contour level is shown as a vertical line and the intersection between the line and the curve gives the volume of the enclosed surface at the given level. Page 154 Full wwPDB EM Validation Report EMD-6695, 5WYJ

7.3 Rotationally averaged power spectrum ○i

*Reported resolution corresponds to spatial frequency of 0.115 Å−1 Page 155 Full wwPDB EM Validation Report EMD-6695, 5WYJ

8 Fourier-Shell correlation ○i

Fourier-Shell Correlation (FSC) is the most commonly used method to estimate the resolution of single-particle and subtomogram-averaged maps. The shape of the curve depends on the imposed symmetry, mask and whether or not the two 3D reconstructions used were processed from a common reference. The reported resolution is shown as a black line. A curve is displayed for the half-bit criterion in addition to lines showing the 0.143 gold standard cut-off and 0.5 cut-off.

8.1 FSC ○i

*Reported resolution corresponds to spatial frequency of 0.115 Å−1 Page 156 Full wwPDB EM Validation Report EMD-6695, 5WYJ

8.2 Resolution estimates ○i

Estimation criterion (FSC cut-off) Resolution estimate (Å) 0.143 0.5 Half-bit Reported by author 8.70 - - Author-provided FSC curve 8.70 10.17 8.85 Calculated* - - -

*Resolution estimate based on FSC curve calculated by comparison of deposited half-maps. Page 157 Full wwPDB EM Validation Report EMD-6695, 5WYJ

9 Map-model fit ○i

This section contains information regarding the fit between EMDB map EMD-6695 and PDB model 5WYJ. Per-residue inclusion information can be found in section3 on page 15.

9.1 Map-model overlay ○i

X Y Z

The images above show the 3D surface view of the map at the recommended contour level 0.09 at 50% transparency in yellow overlaid with a ribbon representation of the model coloured in blue. These images allow for the visual assessment of the quality of fit between the atomic model and the map. Page 158 Full wwPDB EM Validation Report EMD-6695, 5WYJ

9.2 Atom inclusion ○i

At the recommended contour level, 70% of all backbone atoms, 57% of all non-hydrogen atoms, are inside the map.