DMD#43455

Changes in Expression of Drug-Metabolizing Enzymes by Single-walled Carbon nanotubes in Human

Respiratory Tract Cells

Kotaro Hitoshi, Miki Katoh, Tomoko Suzuki, Yoshinori Ando, and Masayuki Nadai

Drug Metabolism and Disposition Supplemental Table 1. Expression levels of drug metabolizing genes after 24-hr exposure of 0.1 mg/ml SWCNT to NHBE cells analyzed by PCR array. Accession Gene Fold change number NM_000927 ATP-binding cassette, sub-family B (MDR/TAP), member 1 BLQ NM_004996 ATP-binding cassette, sub-family C (CFTR/MRP), member 1 0.43 NM_001091 Amiloride binding protein 1 (amine oxidase (copper-containing)) 0.16 NM_000668 Alcohol dehydrogenase IB (class I), beta polypeptide BLQ NM_000669 Alcohol dehydrogenase 1C (class I), gamma polypeptide 2.01 NM_000670 Alcohol dehydrogenase 4 (class II), pi polypeptide 0.11 NM_000671 Alcohol dehydrogenase 5 (class III), chi polypeptide 1.55 NM_000672 Alcohol dehydrogenase 6 (class V) 1.94 NM_001621 Aryl hydrocarbon receptor 1.45 NM_000031 Aminolevulinate, delta-, dehydratase 1.35 NM_000689 Aldehyde dehydrogenase 1 family, member A1 2.99 NM_000697 Arachidonate 12-lipoxygenase 1.42 NM_001140 Arachidonate 15-lipoxygenase 1.52 NM_000698 Arachidonate 5-lipoxygenase 0.69 NM_000041 Apolipoprotein E 0.79 NM_001668 Aryl hydrocarbon receptor nuclear translocator 0.84 NM_004317 ArsA arsenite transporter, ATP-binding, homolog 1 (bacterial) 0.60 NM_000712 Biliverdin reductase A 1.42 NM_000713 Biliverdin reductase B (flavin reductase (NADPH)) 0.99 NM_198061 Carboxylesterase 2 (intestine, liver) 0.44 NM_016280 Carboxylesterase 4-like BLQ NM_003654 Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 BLQ NM_000754 Catechol-O-methyltransferase 0.73 NM_000498 Cytochrome P450, family 11, subfamily B, polypeptide 2 0.33 NM_000102 Cytochrome P450, family 17, subfamily A, polypeptide 1 1.04 NM_000103 Cytochrome P450, family 19, subfamily A, polypeptide 1 0.21 NM_000499 Cytochrome P450, family 1, subfamily A, polypeptide 1 0.00 NM_000767 Cytochrome P450, family 2, subfamily B, polypeptide 6 BLQ NM_000769 Cytochrome P450, family 2, subfamily C, polypeptide 19 1.08 NM_000770 Cytochrome P450, family 2, subfamily C, polypeptide 8 1.20 NM_000771 CYP2C9 Cytochrome P450, family 2, subfamily C, polypeptide 9 1.12 NM_000106 Cytochrome P450, family 2, subfamily D, polypeptide 6 1.48 NM_000773 Cytochrome P450, family 2, subfamily E, polypeptide 1 1.02 NM_000774 Cytochrome P450, family 2, subfamily F, polypeptide 1 BLQ NM_000775 Cytochrome P450, family 2, subfamily J, polypeptide 2 1.46 NM_000777 Cytochrome P450, family 3, subfamily A, polypeptide 5 1.00 NM_007326 Cytochrome b5 reductase 3 0.88 NM_000120 Epoxide hydrolase 1, microsomal (xenobiotic) 0.69 NM_001441 Fatty acid amide hydrolase 0.68 DMD#43455

NM_000507 Fructose-1,6-bisphosphatase 1 0.90 NM_000817 Glutamate decarboxylase 1 (brain, 67kDa) 1.16 NM_001486 Glucokinase (hexokinase 4) regulator 0.27 NM_005265 Gamma-glutamyltransferase 1 0.57 NM_000175 Glucose phosphate isomerase 1.14 NM_000581 Glutathione peroxidase 1 1.60 NM_002083 Glutathione peroxidase 2 (gastrointestinal) 0.91 NM_002084 Glutathione peroxidase 3 (plasma) 0.85 NM_002085 Glutathione peroxidase 4 (phospholipid hydroperoxidase) 0.84 NM_001509 Glutathione peroxidase 5 (epididymal androgen-related protein) BLQ NM_000637 Glutathione reductase 0.32 NM_000847 Glutathione S-transferase A3 BLQ NM_001512 Glutathione S-transferase A4 2.12 NM_000848 Glutathione S-transferase M2 (muscle) 1.06 NM_000849 Glutathione S-transferase M3 (brain) 0.35 NM_000851 Glutathione S-transferase M5 0.67 NM_000852 Glutathione S-transferase pi 1.01 NM_000853 Glutathione S-transferase theta 1 0.78 NM_001513 Glutathione transferase zeta 1 (maleylacetoacetate isomerase) 0.82 NM_000189 Hexokinase 2 0.47 NM_000413 Hydroxysteroid (17-beta) dehydrogenase 1 0.57 NM_002153 Hydroxysteroid (17-beta) dehydrogenase 2 0.48 NM_000197 Hydroxysteroid (17-beta) dehydrogenase 3 2.05 NM_006151 Lactoperoxidase BLQ NM_002356 Myristoylated alanine-rich protein kinase C substrate 0.99 NM_020300 Microsomal glutathione S-transferase 1 0.99 NM_002413 Microsomal glutathione S-transferase 2 1.33 NM_004528 Microsomal glutathione S-transferase 3 1.42 NM_000250 Myeloperoxidase BLQ NM_005953 Metallothionein 2A 0.54 NM_005954 Metallothionein 3 0.46 NM_005957 5,10-methylenetetrahydrofolate reductase (NADPH) 0.49 NM_000662 N-acetyltransferase 1 (arylamine N-acetyltransferase) 1.43 NM_000015 N-acetyltransferase 2 (arylamine N-acetyltransferase) 1.20 NM_000603 Nitric oxide synthase 3 (endothelial cell) 0.88 NM_000903 NAD(P)H dehydrogenase, quinone 1 1.03 NM_000298 Pyruvate kinase, liver and RBC 0.31 NM_002654 Pyruvate kinase, muscle 0.82 NM_000446 Paraoxonase 1 BLQ NM_000305 Paraoxonase 2 1.29 NM_000940 Paraoxonase 3 0.84 NM_014140 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, 1.12 subfamily a-like 1 NM_003498 Stannin 0.69 NM_001047 Steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 1.65 4-dehydrogenase alpha 1) NM_000348 Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta BLQ 4-dehydrogenase alpha 2) BLQ: below the limit of quantification