Recombinant Human Biliverdin Reductase B/BLVRB Protein Catalog Number: BVR0901

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Recombinant human Biliverdin Reductase B/BLVRB protein Catalog Number: BVR0901 PRODUCT INPORMATION Expression system E.coli Domain 1-206aa UniProt No. P30043 NCBI Accession No. NP_000704.1 Alternative Names BLVRB, FLR, BVRB, SDR43u1, MGC117413, Biliverdin reductase B, Biliverdin IX beta reductase, BVR B, Flavin reductase, Flavin reductase (NADPH), FR, GHBP, Green heme binding protein, MGC117413, NADPH dependent diaphorase, NADPH flavin reductase. PRODUCT SPECIFICATION Molecular Weight 22.1 kDa (206aa) confirmed by MALDI-TOF Concentration 1mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT Purity > 95% by SDS-PAGE Tag Non-Tagged Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Biliverdin reductase B (BLVRB) is an enzyme (EC 1. 3. 1. 24) that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. BLVRB is found that major erythrocytic heme catabolic pathway in humans and most mammalian species. Biliverdin reductase is abundantly expressed in kidney, spleen, liver and brain as well as at lower levels in the thymus and minimal 1 Recombinant human Biliverdin Reductase B/BLVRB protein Catalog Number: BVR0901 levels being detected in testis. Recombinant BLVRB protein was expressed in E. coli and purified by using conventional chromatography techniques. Amino acid Sequence MAVKKIAIFG ATGQTGLTTL AQAVQAGYEV TVLVRDSSRL PSEGPRPAHV VVGDVLQAAD VDKTVAGQDA VIVLLGTRND LSPTTVMSEG ARNIVAAMKA HGVDKVVACT SAFLLWDPTK VPPRLQAVTD DHIRMHKVLR ESGLKYVAVM PPHIGDQPLT GAYTVTLDGR GPSRVISKHD LGHFMLRCLT TDEYDGHSTY PSHQYQ General References L. Lee Grismer., et al. (2007). Herpetologica. 63(3):392-400 Baranano DE., et al. (2002). Proc Natl Acad Sci uSA. 99(25):16093-8 DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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1 Metabolic Dysfunction Is Restricted to the Sciatic Nerve in Experimental
Page 1 of 255 Diabetes Metabolic dysfunction is restricted to the sciatic nerve in experimental diabetic neuropathy Oliver J. Freeman1,2, Richard D. Unwin2,3, Andrew W. Dowsey2,3, Paul Begley2,3, Sumia Ali1, Katherine A. Hollywood2,3, Nitin Rustogi2,3, Rasmus S. Petersen1, Warwick B. Dunn2,3†, Garth J.S. Cooper2,3,4,5* & Natalie J. Gardiner1* 1 Faculty of Life Sciences, University of Manchester, UK 2 Centre for Advanced Discovery and Experimental Therapeutics (CADET), Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, UK 3 Centre for Endocrinology and Diabetes, Institute of Human Development, Faculty of Medical and Human Sciences, University of Manchester, UK 4 School of Biological Sciences, University of Auckland, New Zealand 5 Department of Pharmacology, Medical Sciences Division, University of Oxford, UK † Present address: School of Biosciences, University of Birmingham, UK *Joint corresponding authors: Natalie J. Gardiner and Garth J.S. Cooper Email: [email protected]; [email protected] Address: University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, United Kingdom Telephone: +44 161 275 5768; +44 161 701 0240 Word count: 4,490 Number of tables: 1, Number of figures: 6 Running title: Metabolic dysfunction in diabetic neuropathy 1 Diabetes Publish Ahead of Print, published online October 15, 2015 Diabetes Page 2 of 255 Abstract High glucose levels in the peripheral nervous system (PNS) have been implicated in the pathogenesis of diabetic neuropathy (DN). However our understanding of the molecular mechanisms which cause the marked distal pathology is incomplete. Here we performed a comprehensive, system-wide analysis of the PNS of a rodent model of DN.
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Supp Table 6.Pdf
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