Bastian et al. 2012

SUPPLEMENTARY TABLES

Synergistic activity of bortezomib and HDACis in preclinical models of B-cell precursor acute lymphoblastic leukemia via modulation of p53, PI3K/AKT and NF-B

Lorenz Bastian, Jana Hof, Madlen Pfau, Iduna Fichtner, Cornelia Eckert, Günter Henze, Javier Prada, Arend von Stackelberg, Karl Seeger and Shabnam Shalapour

0 Bastian et al. 2012

Supplementary Table S1. Fractional Product of Webb calculations for concomitant combinations of HDACis (VPA, SAHA) and bortezomib or chemotherapeutic agents in Nalm6 cells.

VPA SAHA

bortezomib 6 nM 2.73 3.47

8 nM 1.23 1.27

idarubicin 10 nM 1.56 1.51

20 nM 1.19 1.22

cytarabine 20 nM 1.53 1.29

80 nM 0.85 0.83

vincristine 3 nM 1.53 1.89

5 nM 0.90 0.99

Nalm6 cells were treated for 72 h with either BTZ or chemotherapeutic agents alone or in combination with HDACis (VPA, SAHA). BTZ and chemotherapeutics were applied at moderate and submaximal effective dose levels as indicated. Apoptosis was assessed by FACS analysis of annexin-V/PI stained cells. Results are shown in

Supplementary Figure S2A.

Fractional product method of Webb relates a measured effect of combined treatment to an expected effect calculated from the effects of treatment with single drugs.

Values <1 indicate antagonism, ~ 1 indicate additivity, > 1 indicate synergism.

Abbreviations: VPA, valproic acid; SAHA, suberoylanilide hydroxamic acid.

1 Bastian et al. 2012

Supplementary Table S2. Sensitivities of Reh, Nalm6 and 697 cells to treatments with VPA and SAHA.

VPA (mM) SAHA (μM)

Reh Ic25 3.6 1.3

Ic50 > 6 > 2.4 Nalm6 Ic25 2.1 1.6

Ic50 > 3 2.2

697 Ic25 1.5 0.9

Ic50 2.1 1.1

Cell lines were treated with serial dilutions of VPA or SAHA. After 72 h apoptosis was measured by FACS analysis of annexin-V / PI stained cells, as indicated in

Supplementary Figure S1B-D. The 25% and 50% effective concentrations were determined by linear regression analysis.

2 Bastian et al. 2012

Supplementary Table S3. Fractional Product of Webb calculations for concomitant combinations of BTZ and VPA in 697, SD-1 and SEM cells at three different time points.

VPA (24 h) VPA (48 h) VPA (72 h)

697 BTZ (10 nM) 4.21 5.34 12.36 BTZ (14 nM) 2.74 2.86 4.30

SD-1 BTZ (10 nM) 1.67 2.54 36.91 BTZ (12 nM) 1.12 1.01 1.19

SEM BTZ (2 nM) 1.20 1.51 1.60 BTZ (4 nM) 1.61 1.31 1.20

697, SD-1 and SEM cells were exposed to BTZ at two concentrations, combined concomitantly with or without VPA. After 24 h, 48 h and 72 h of treatment, cytotoxicity was assessed by FACS analysis of PI stained cells. The IC 50 concentrations determined for BTZ single treatments after 48h were 18.2 nM, 10.5 nM and 4.0 nM for 697, SD-1 and SEM respectively.

Fractional product method of Webb relates a measured effect of combined treatment to an expected effect calculated from the effects of treatment with single drugs.

Values <1 indicate antagonism, ~ 1 indicate additivity, > 1 indicate synergism.

Abbreviations: BTZ, bortezomib. VPA, valproic acid.

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Supplementary Table S4. Fractional Product of Webb calculations for concomitant and sequential combinations of BTZ and HDACis (VPA, SAHA) in

Reh and Nalm6 cells.

BTZ + HDACi BTZ + HDACi BTZ b HDACi HDACi b BTZ HDACi (36 h) (72 h)

Reh VPA 5.36 5.82 1.28 0.30 SAHA 4.94 4.44 1.12 0.88

Nalm6 VPA n.d. 3.79 0.89 0.59 SAHA n.d. 3.17 1.31 0.99

Reh and Nalm6 cells were treated with BTZ for 36 h, washed and resuspended in fresh media containing HDACis (VPA, SAHA) for further 36 h or the reversed order of drug application. As controls were used: treatments with single drugs for 36 h, after which cells were washed and resuspended in fresh media without drugs for further

36 h or treatments with the combination of HDACI/BTZ for 36 h, after which cells were washed and resuspended again for further 36 h in media containing the

HDACI/BTZ combination. After 72 h cells were stained with Annexin-V/PI and the proportions of apoptotic cells were determined by FACS. Apoptosis rates in Reh cells after 36 h of treatment with VPA/BTZ and SAHA/BTZ were 89.8% (±0.4) and 72.9%

(± 3.6) respectively. Representative results are shown in Figure 1C.

Fractional product method of Webb relates a measured effect of combined treatment to an expected effect calculated from the effects of treatment with single drugs.

Values <1 indicate antagonism, ~ 1 indicate additivity, > 1 indicate synergism.

Abbreviations: BTZ, bortezomib; VPA, valproic acid; SAHA, suberoylanilide hydroxamic acid; n.d. not determined.

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Supplementary Table S5. NF-B subunits gene expression in leukemia cells in

correlation with the clinical and biological characteristics of the BCP-ALL

relapse patient cohort.

Parameter Total RelA RelB NFKB2 NFKB1 Total N median (min;max) p-value median (min;max) p-value median (min;max) p-value median (min;max) p-value gender 0.16 0.36 0.24 0.25 male 36 8.25(7.40; 8.97) 7.57(6.39;9.4) 7.42(6.66;8.50) 9(8.25;10.61) female 16 8.18 (7.56;8.56) 7.37(6.17;8.75) 7.05(6.51;8.81) 8.75(7.64;10.61) age at relapse 0.80 0.28 0.38 0.09

< 5 years 7 8.34(7.84;8.62) 7.79(6.88;8.75) 7.03(6.86;8.50) 8.63(8.35;9.62) 5 and < 10 years 22 8.12(7.40;8.96) 7.6(6.17;9.4) 7.49(6.61;8.81) 9.2(8.43;10.61) 10 years 23 8.27(7.56;8.97) 7.33(6.57;8.32) 7.24(6.51;8.40) 8.97(7.64;9.99) timepoint of relapse 0.03 0.05 0.03 0.05 very early 14 8.15(7.46;8.55) 7.22(6.57;8.42) 6.9(6.6;8.5) 8.75(8.25;9.62) early 10 8.05(7.40;8.62) 7.46(6.39;8.4) 7.25(6.81;8.45) 8.87(7.64;10.69) late 28 8.35(7.56;8.97) 7.65(6.17;9.4) 7.5(6.51;8.81) 9.12(8.35;10.61) relapse on/off initial treatment 0.006 0.01 0.008 0.03 on treatment 18 8.05(7.46;8.62) 7.22(6.57;8.42) 7.02(6.60;8.50) 8.82(7.64;10.59) off treatment 34 8.35(7.40;8.97) 7.65(6.17;9.4) 7.5(6.51;8.81) 9.08(8.35;10.61) site of relapse 0.24 0.19 0.19 0.43 BM isolated 45 8.21(7.40;8.96) 7.44(6.17;9.4) 7.26(6.51;8.81) 8.96(7.64;10.61) BM combined 7 8.36(7.99;8.97) 7.88(7.43;8.4) 7.44(7.07;8.49) 9.11(8.76;9.89) immunophenotype 0.60 0.18 0.35 0.57 Pro–B-cell ALL 4 8.44(7.93;8.69) 7.85(7.58;8.4) 7.53(6.97;8.49) 9.71(8.35;9.89) Common ALL 29 8.17(7.46;8.97) 7.44(6.17;9.4) 7.14(6.51;8.48) 8.9(7.64;10.61) Pre-B-cell ALL 15 8.3(7.40;8.84) 7.43(6.39;8.59) 7.43(6.74;8.81) 9.01(8.25;10.61) BCP-ALL not specified 4 response to treatment 0.44 0.22 0.04 0.86 early/normal 31 8.27(7.40;8.97) 7.58(6.17;9.4) 7.53(6.51;8.81) 8.93(8.25;10.61) late/non-response 18 8.18(7.76;8.61) 7.33(6.7;8.9) 7.05(6.61;8.48) 8.99(7.64;10.69) (no data) 3 Outcome 0.63 0.59 0.48 0.23 CCR 13 8.16(7.40;8.97) 7.9(6.17;9.4) 7.26(6.51;8.47) 8.81(8.4;9.96) death in CR 3 8.61(8.07;8.72) 7.01(6.9;7.37) 7.05(6.74;7.77) 8.82(8.59;9.06) second relapse 24 8.32(7.46;8.94) 7.62(6.57;8.59) 7.5(6.61;8.81) 9.12(7.64;10.61) non-response 7 8.27(7.85;8.42) 7.42(6.7;8.9) 7.24(6.9;8.48) 9.17(8.38;9.77) induction death 1 7.93(-;-) 7.12(-;-) 6.6(-;-) 8.37(-;-) (non protocol) 4 second event 0.67 0.79 0.70 0.20 no 13 8.16(7.40;8.97) 7.9(6.17;9.4) 7.26(6.51;8.47) 8.81(8.4;9.96) yes 35 8.31(7.46;8.94) 7.48(6.57;8.9) 7.4(6.6;8.81) 9.06(7.64;10.61) (non protocol) 4

Abbreviations: BCP, B-cell precursor; ALL, acute lymphoblastic leukemia; BM, bone

marrow; CR, complete remission; CCR, continuous complete remission. Time point

of relapse: very early (within 18 months after first diagnosis of ALL), early (18 months

after first diagnosis of ALL until 6 months after cessation of frontline therapy), or late

(more than 6 months after cessation of frontline therapy). Relapse on/off treatment:

5 Bastian et al. 2012 on treatment (during frontline chemotherapy) or off treatment (after cessation of frontline chemotherapy). Site of relapse: isolated BM (more than 25% leukemia cells in BM, no evidence of extramedullary disease) or combined BM (more than 5% leukemia cells in the BM combined with CNS, testis or other extramedullary sites).

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Supplementary Table S6. Detailed information on the mortality in the s.c.- and

i.v.- xenograft mouse model experiments.

control VPA BTZ BTZ BTZ BTZ BTZ 250 0.4 0.6 0.8 0.6 0.8 mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg + VPA + VPA s.c. - dose finding 0/8 0/8 0/8 0/8 model experiment

combination 0/8 0/8 3/8 2/8 experiment i.v. - combination 2/8 1/8 1/8 1/8 model experiment

Total 2/24 1/24 0/8 1/8 3/16 1/8 2/8

p=1.0 p=1.0 p=1.0 p=0.373 p=1.0 p=0.254

The table provides a summary of events (death) during the three xenograft mouse

model experiments. The corresponding experiments are described in detail as

follows: s.c. – xenograft mouse model: dose finding study – Supplementary Figure

S11A-C, combination experiment: Figure 5C-D and Supplementary Figure S11D-F;

i.v. – xenograft mouse model: Figure 5B and Supplementary Figure 10. p-values

obtained in the experiments: Fisher’s exact test.

7 Bastian et al. 2012

Supplementary Table S7. Differential expression, fold change and statistical

analysis of the 484 genes upregulated in Reh cells only after concomitant

treatment with VPA and BTZ.

Sequence Primary Sequence Description [Accession number] Fold p-value Code Sequence Change Name

A_33_P3395581 TRIM53 Homo sapiens tripartite motif-containing 53 (TRIM53), non-coding RNA [NR_028346] 17.98 2.79E-10 A_33_P3394858 NCRNA00092 Homo sapiens non-protein coding RNA 92 (NCRNA00092), non-coding RNA [NR_024129] 17.61 2.03E-10 A_32_P199462 C2orf80 Homo sapiens chromosome 2 open reading frame 80 (C2orf80), mRNA [NM_001099334] 17.33 1.93E-15 A_23_P37088 RDH12 Homo sapiens retinol dehydrogenase 12 (all-trans/9-cis/11-cis) (RDH12), mRNA [NM_152443] 13.91 2.13E-20 A_24_P315056 C10orf122 Homo sapiens chromosome 10 open reading frame 122 (C10orf122), mRNA [NM_001128202] 13.46 1.60E-10 A_23_P46315 DENND2C DENN/MADD domain containing 2C [Source:HGNC Symbol;Acc:24748] [ENST00000369540] 10.28 2.67E-09 A_24_P366607 C13orf36 Homo sapiens chromosome 13 open reading frame 36 (C13orf36), mRNA [NM_203451] 9.09 1.93E-06 A_23_P44155 CD96 Homo sapiens CD96 molecule (CD96), transcript variant 1, mRNA [NM_198196] 7.91 1.00E-05 A_33_P3230389 OR52N2 Homo sapiens olfactory receptor, family 52, subfamily N, member 2 (OR52N2), mRNA [NM_001005174] 7.89 1.00E-05

A_23_P397293 LY6K Homo sapiens lymphocyte antigen 6 complex, locus K (LY6K), transcript variant 1, mRNA [NM_017527] 7.70 3.00E-05

A_23_P257971 AKR1C1 Homo sapiens aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)- 7.46 4.51E-13 hydroxysteroid dehydrogenase) (AKR1C1), mRNA [NM_001353] A_33_P3397995 IPCEF1 Homo sapiens interaction protein for cytohesin exchange factors 1 (IPCEF1), transcript variant 1, mRNA 7.30 5.00E-05 [NM_001130699] A_23_P82047 STXBP5 Homo sapiens syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2, mRNA 7.11 8.00E-05 [NM_001127715] A_32_P33576 OPRK1 Homo sapiens opioid receptor, kappa 1 (OPRK1), mRNA [NM_000912] 6.96 4.38E-08 A_33_P3229239 HIST2H2BF Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1, mRNA [NM_001024599] 6.56 2.60E-04

A_23_P90626 CYTIP Homo sapiens cytohesin 1 interacting protein (CYTIP), mRNA [NM_004288] 6.54 3.10E-04 A_33_P3382143 STARD13 Homo sapiens StAR-related lipid transfer (START) domain containing 13 (STARD13), transcript variant 6.32 4.40E-04 alpha, mRNA [NM_178006] A_23_P350451 PRDM1 Homo sapiens PR domain containing 1, with ZNF domain (PRDM1), transcript variant 1, mRNA 6.18 5.29E-11 [NM_001198] A_23_P140748 NDRG4 Homo sapiens NDRG family member 4 (NDRG4), transcript variant 3, mRNA [NM_022910] 6.12 3.02E-16 A_23_P139864 GSG1 Homo sapiens germ cell associated 1 (GSG1), transcript variant 1, mRNA [NM_031289] 5.92 1.03E-03 A_23_P61637 HAL Homo sapiens histidine ammonia-lyase (HAL), mRNA [NM_002108] 5.69 3.42E-06 A_23_P420863 NOD2 Homo sapiens nucleotide-binding oligomerization domain containing 2 (NOD2), mRNA [NM_022162] 5.61 1.00E-05

A_23_P256965 C8orf12 Homo sapiens chromosome 8 open reading frame 12 (C8orf12), non-coding RNA [NR_026814] 5.48 2.49E-03 A_23_P387585 NCRNA00246 Homo sapiens non-protein coding RNA 246 (NCRNA00246), non-coding RNA [NR_026595] 5.45 2.71E-03 A_23_P206140 DNAJA4 Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4), transcript variant 1, mRNA 5.30 1.00E-04 [NM_018602] A_23_P216448 NFIB Homo sapiens nuclear factor I/B (NFIB), transcript variant 3, mRNA [NM_005596] 5.27 4.20E-03 A_23_P52336 UNC5B Homo sapiens unc-5 homolog B (C. elegans) (UNC5B), mRNA [NM_170744] 5.23 4.23E-07 A_33_P3297092 H2BFWT Homo sapiens H2B histone family, member W, testis-specific (H2BFWT), mRNA [NM_001002916] 5.14 7.70E-04

A_33_P3338341 PRODH Homo sapiens proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, 5.08 6.57E-13 transcript variant 1, mRNA [NM_016335] A_23_P58082 CCDC80 Homo sapiens coiled-coil domain containing 80 (CCDC80), transcript variant 1, mRNA [NM_199511] 5.01 6.11E-03

A_23_P404481 S1PR1 Homo sapiens sphingosine-1-phosphate receptor 1 (S1PR1), mRNA [NM_001400] 4.97 1.30E-04 A_23_P118203 ZG16B Homo sapiens zymogen granule protein 16 homolog B (rat) (ZG16B), mRNA [NM_145252] 4.94 6.93E-03 A_24_P257579 EPB41L4A Homo sapiens erythrocyte membrane protein band 4.1 like 4A (EPB41L4A), mRNA [NM_022140] 4.93 6.98E-03 A_23_P501985 CSF2RA Homo sapiens colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), 4.91 1.60E-04 transcript variant 6, mRNA [NM_172249] A_32_P176911 NCRNA00239 Homo sapiens non-protein coding RNA 239 (NCRNA00239), non-coding RNA [NR_026774] 4.90 5.33E-03 A_23_P164196 DLX4 Homo sapiens distal-less homeobox 4 (DLX4), transcript variant 1, mRNA [NM_138281] 4.84 2.10E-04 A_24_P148750 SH3BP5 Homo sapiens SH3-domain binding protein 5 (BTK-associated) (SH3BP5), transcript variant 1, mRNA 4.79 8.79E-03 [NM_004844] A_33_P3366584 OR56A4 Homo sapiens olfactory receptor, family 56, subfamily A, member 4 (OR56A4), mRNA [NM_001005179] 4.78 5.23E-03

A_32_P170547 CT45A5 Homo sapiens cancer/testis antigen family 45, member A5 (CT45A5), transcript variant 1, mRNA 4.72 5.11E-03 [NM_001007551] A_23_P29773 LAMP3 Homo sapiens lysosomal-associated membrane protein 3 (LAMP3), mRNA [NM_014398] 4.69 1.03E-16 A_33_P3401981 SPRED3 Homo sapiens sprouty-related, EVH1 domain containing 3 (SPRED3), transcript variant 2, mRNA 4.64 2.80E-04 [NM_001039616] A_23_P346912 SPATA17 Homo sapiens spermatogenesis associated 17 (SPATA17), mRNA [NM_138796] 4.55 2.02E-03 A_33_P3291092 THSD1 Homo sapiens thrombospondin, type I, domain containing 1 (THSD1), transcript variant 2, mRNA 4.50 1.91E-03 [NM_199263] A_23_P82979 LAMC3 Homo sapiens laminin, gamma 3 (LAMC3), mRNA [NM_006059] 4.47 2.76E-13

8 Bastian et al. 2012

A_23_P259071 AREG Homo sapiens amphiregulin (AREG), mRNA [NM_001657] 4.22 6.40E-04 A_23_P161458 OLAH Homo sapiens oleoyl-ACP hydrolase (OLAH), transcript variant 2, mRNA [NM_001039702] 4.21 2.30E-04 A_33_P3316456 NPL Homo sapiens N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 4.10 4.29E-09 2, mRNA [NM_001200050] A_24_P115932 GPR44 Homo sapiens G protein-coupled receptor 44 (GPR44), mRNA [NM_004778] 3.98 2.62E-03 A_33_P3839897 RNU4ATAC HHAGE001732 Human liver regeneration after partial hepatectomy Homo sapiens cDNA, mRNA sequence 3.97 6.97E-13 [DW419002] A_23_P341527 DCST1 Homo sapiens DC-STAMP domain containing 1 (DCST1), transcript variant 1, mRNA [NM_152494] 3.95 1.10E-04

A_33_P3313779 CCDC64 Homo sapiens coiled-coil domain containing 64 (CCDC64), mRNA [NM_207311] 3.84 7.14E-03 A_32_P4199 RNF152 Homo sapiens ring finger protein 152 (RNF152), mRNA [NM_173557] 3.74 1.58E-06 A_23_P394259 MPP2 Homo sapiens membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), mRNA 3.67 4.57E-03 [NM_005374] A_24_P273742 TTC9 Homo sapiens tetratricopeptide repeat domain 9 (TTC9), mRNA [NM_015351] 3.66 9.20E-04 A_33_P3395848 VSIG10L Homo sapiens V-set and immunoglobulin domain containing 10 like (VSIG10L), mRNA [NM_001163922] 3.64 1.00E-05

A_23_P69586 FAT1 Homo sapiens FAT tumor suppressor homolog 1 (Drosophila) (FAT1), mRNA [NM_005245] 3.58 2.69E-03 A_32_P158083 LHFPL3 Homo sapiens lipoma HMGIC fusion partner-like 3 (LHFPL3), mRNA [NM_199000] 3.51 8.50E-03 A_23_P168592 CCDC126 Homo sapiens coiled-coil domain containing 126 (CCDC126), mRNA [NM_138771] 3.50 1.91E-11 A_33_P3268487 ABAT Homo sapiens 4-aminobutyrate aminotransferase (ABAT), nuclear gene encoding mitochondrial protein, 3.42 8.21E-22 transcript variant 2, mRNA [NM_000663] A_33_P3279550 MIXL1 Homo sapiens Mix1 homeobox-like 1 (Xenopus laevis) (MIXL1), mRNA [NM_031944] 3.40 7.75E-03 A_23_P54846 HERPUD1 Homo sapiens homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain 3.39 1.09E-21 member 1 (HERPUD1), transcript variant 1, mRNA [NM_014685] A_24_P200023 IL1R1 Homo sapiens interleukin 1 receptor, type I (IL1R1), mRNA [NM_000877] 3.37 3.05E-03 A_33_P3225690 ZNF516 Homo sapiens zinc finger protein 516 (ZNF516), mRNA [NM_014643] 3.36 2.43E-11 A_23_P36641 AICDA Homo sapiens activation-induced cytidine deaminase (AICDA), mRNA [NM_020661] 3.26 4.48E-03 A_33_P3374289 C10orf82 Homo sapiens chromosome 10 open reading frame 82 (C10orf82), mRNA [NM_144661] 3.25 4.17E-03 A_23_P114947 RGS2 Homo sapiens regulator of G-protein signaling 2, 24kDa (RGS2), mRNA [NM_002923] 3.25 0.00E+00 A_23_P431252 KBTBD8 Homo sapiens kelch repeat and BTB (POZ) domain containing 8 (KBTBD8), mRNA [NM_032505] 3.24 7.98E-11

A_33_P3414912 NPL Homo sapiens N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), transcript variant 3.20 1.80E-04 3, mRNA [NM_001200056] A_33_P3306103 CALCRL Homo sapiens calcitonin receptor-like (CALCRL), mRNA [NM_005795] 3.20 5.19E-10 A_23_P4808 PTGER1 Homo sapiens prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1), mRNA [NM_000955] 3.19 1.23E-03

A_33_P3276207 FAM55C Homo sapiens family with sequence similarity 55, member C (FAM55C), transcript variant 1, mRNA 3.17 7.48E-11 [NM_001134456] A_23_P17065 CCL20 Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 1, mRNA [NM_004591] 3.16 1.42E-06

A_33_P3407339 C6orf204 Homo sapiens chromosome 6 open reading frame 204 (C6orf204), transcript variant 2, mRNA 3.15 9.36E-08 [NM_206921] A_23_P135486 AHSP Homo sapiens alpha hemoglobin stabilizing protein (AHSP), mRNA [NM_016633] 3.14 4.24E-03 A_23_P96285 REEP1 Homo sapiens receptor accessory protein 1 (REEP1), nuclear gene encoding mitochondrial protein, 3.13 1.23E-17 transcript variant 2, mRNA [NM_022912] A_23_P139143 STX3 Homo sapiens syntaxin 3 (STX3), transcript variant 1, mRNA [NM_004177] 3.12 6.40E-20 A_23_P329261 KCNJ2 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 2 (KCNJ2), mRNA 3.11 7.10E-20 [NM_000891] A_33_P3371224 ANO10 Homo sapiens anoctamin 10 (ANO10), mRNA [NM_018075] 3.11 1.21E-10 A_24_P89457 CDKN1A Homo sapiens cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 2, mRNA 3.10 1.01E-19 [NM_078467] A_23_P75800 RAB3IL1 Homo sapiens RAB3A interacting protein (rabin3)-like 1 (RAB3IL1), mRNA [NM_013401] 3.10 7.44E-10 A_23_P123071 CAV2 Homo sapiens caveolin 2 (CAV2), transcript variant 1, mRNA [NM_001233] 3.10 0.00E+00 A_23_P133842 HIST1H1T Homo sapiens histone cluster 1, H1t (HIST1H1T), mRNA [NM_005323] 3.10 3.44E-09 A_33_P3401753 FFAR3 Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA [NM_005304] 3.09 1.09E-03 A_24_P237778 MAN1C1 Homo sapiens mannosidase, alpha, class 1C, member 1 (MAN1C1), mRNA [NM_020379] 3.08 7.09E-03 A_23_P75088 PDZD7 Homo sapiens PDZ domain containing 7 (PDZD7), transcript variant 2, mRNA [NM_024895] 3.07 1.20E-06 A_23_P53370 RND1 Homo sapiens Rho family GTPase 1 (RND1), mRNA [NM_014470] 3.02 1.17E-16 A_33_P3267263 RNU1-5 Homo sapiens RNA, U1 small nuclear 5 (RNU1-5), small nuclear RNA [NR_004400] 3.01 2.42E-10 A_24_P208567 IL18R1 Homo sapiens interleukin 18 receptor 1 (IL18R1), mRNA [NM_003855] 3.00 2.21E-03 A_24_P385190 SLC4A1 Homo sapiens solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, 3.00 3.41E-03 Diego blood group) (SLC4A1), mRNA [NM_000342] A_23_P104109 RPS6KC1 Homo sapiens ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 1, mRNA 2.98 0.00E+00 [NM_012424] A_24_P212481 MCTP1 Homo sapiens multiple C2 domains, transmembrane 1 (MCTP1), transcript variant L, mRNA [NM_024717] 2.98 6.90E-04

A_33_P3247624 REP15 Homo sapiens RAB15 effector protein (REP15), mRNA [NM_001029874] 2.96 1.43E-09 A_24_P167642 GCH1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA [NM_000161] 2.95 1.16E-18 A_33_P3649236 FLJ39534 Homo sapiens cDNA FLJ33945 fis, clone CTONG2018279. [AK091264] 2.95 9.90E-04 A_24_P49190 C17orf58 Homo sapiens chromosome 17 open reading frame 58 (C17orf58), transcript variant 1, mRNA 2.93 5.64E-10 [NM_181655] A_33_P3386288 SCD5 Homo sapiens stearoyl-CoA desaturase 5 (SCD5), transcript variant 2, mRNA [NM_024906] 2.91 9.54E-03 A_23_P405873 C9orf72 Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 2, mRNA [NM_145005] 2.91 8.75E-09

A_33_P3263867 P2RX7 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, mRNA 2.91 1.87E-18 [NM_002562] A_23_P88580 ARID3B Homo sapiens AT rich interactive domain 3B (BRIGHT-like) (ARID3B), mRNA [NM_006465] 2.90 5.69E-10 A_33_P3240200 DPCD Homo sapiens deleted in primary ciliary dyskinesia homolog (mouse) (DPCD), mRNA [NM_015448] 2.88 3.00E-04

9 Bastian et al. 2012

A_23_P168828 KLF10 Homo sapiens Kruppel-like factor 10 (KLF10), transcript variant 1, mRNA [NM_005655] 2.87 3.29E-18 A_24_P243776 ZNF277 Homo sapiens zinc finger protein 277 (ZNF277), mRNA [NM_021994] 2.87 7.84E-10 A_33_P3385775 ZNF703 Homo sapiens zinc finger protein 703 (ZNF703), mRNA [NM_025069] 2.85 2.18E-09 A_32_P69930 CCDC96 Homo sapiens coiled-coil domain containing 96 (CCDC96), mRNA [NM_153376] 2.82 1.26E-09 A_23_P145965 TPST1 Homo sapiens tyrosylprotein sulfotransferase 1 (TPST1), mRNA [NM_003596] 2.81 1.12E-17 A_33_P3397336 ZNF461 Homo sapiens zinc finger protein 461 (ZNF461), mRNA [NM_153257] 2.81 2.03E-03 A_33_P3286157 TNFRSF4 Homo sapiens tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), mRNA [NM_003327] 2.81 1.17E-10

A_33_P3371089 SGTB Homo sapiens small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta (SGTB), mRNA 2.81 1.35E-09 [NM_019072] A_33_P3415092 CLCN5 Homo sapiens chloride channel 5 (CLCN5), transcript variant 1, mRNA [NM_001127899] 2.80 5.19E-16 A_23_P62932 ATP1B1 Homo sapiens ATPase, Na+/K+ transporting, beta 1 polypeptide (ATP1B1), mRNA [NM_001677] 2.80 1.10E-17 A_23_P82351 BBS9 Homo sapiens Bardet-Biedl syndrome 9 (BBS9), transcript variant 2, mRNA [NM_198428] 2.80 1.10E-17 A_23_P355377 SLC12A5 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript 2.78 1.69E-03 variant 2, mRNA [NM_020708] A_23_P256641 KCNE1L Homo sapiens KCNE1-like (KCNE1L), mRNA [NM_012282] 2.78 4.52E-09 A_23_P138805 CHORDC1 Homo sapiens cysteine and histidine-rich domain (CHORD)-containing 1 (CHORDC1), transcript variant 1, 2.78 1.73E-09 mRNA [NM_012124] A_23_P6344 SDF2L1 Homo sapiens stromal cell-derived factor 2-like 1 (SDF2L1), mRNA [NM_022044] 2.77 1.84E-09 A_33_P3327592 RNF13 Homo sapiens ring finger protein 13 (RNF13), transcript variant 1, mRNA [NM_007282] 2.76 1.97E-09 A_33_P3279708 RNU2-2 Homo sapiens RNA, U2 small nuclear 2 (RNU2-2), small nuclear RNA [NR_002761] 2.76 2.21E-17 A_23_P200096 SPSB1 Homo sapiens splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1), mRNA [NM_025106] 2.76 1.34E-07

A_24_P68222 CD99P1 Homo sapiens CD99 molecule pseudogene 1 (CD99P1), transcript variant 2, non-coding RNA [NR_033381] 2.76 1.50E-08

A_23_P387630 STARD8 Homo sapiens StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2, 2.76 9.70E-04 mRNA [NM_014725] A_23_P350074 C19orf46 Homo sapiens chromosome 19 open reading frame 46 (C19orf46), mRNA [NM_001039876] 2.75 2.21E-09 A_23_P372834 AQP1 Homo sapiens aquaporin 1 (Colton blood group) (AQP1), transcript variant 1, mRNA [NM_198098] 2.74 4.90E-03

A_23_P67896 SCN3A Homo sapiens sodium channel, voltage-gated, type III, alpha subunit (SCN3A), transcript variant 1, mRNA 2.74 6.24E-16 [NM_006922] A_23_P153320 ICAM1 Homo sapiens intercellular adhesion molecule 1 (ICAM1), mRNA [NM_000201] 2.73 0.00E+00 A_24_P261567 GDPD5 Homo sapiens glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5), mRNA 2.73 1.19E-08 [NM_030792] A_33_P3461948 CRYGN Homo sapiens crystallin, gamma N (CRYGN), mRNA [NM_144727] 2.72 7.96E-03 A_33_P3268035 LYZL1 Homo sapiens lysozyme-like 1 (LYZL1), mRNA [NM_032517] 2.72 8.30E-04 A_32_P149251 DNAJC18 Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 18 (DNAJC18), mRNA [NM_152686] 2.71 3.45E-09

A_33_P3218188 SPSB2 Homo sapiens splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2), transcript variant 1, 2.71 3.37E-09 mRNA [NM_032641] A_23_P315964 UMODL1 Homo sapiens uromodulin-like 1 (UMODL1), transcript variant 2, mRNA [NM_173568] 2.70 1.17E-08 A_23_P92650 SLC25A2 Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 2 (SLC25A2), 2.69 6.88E-03 nuclear gene encoding mitochondrial protein, mRNA [NM_031947] A_23_P29046 CBR1 Homo sapiens carbonyl reductase 1 (CBR1), mRNA [NM_001757] 2.69 8.26E-17 A_32_P137920 RSPH4A Homo sapiens radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1, mRNA 2.69 8.20E-04 [NM_001010892] A_23_P433369 HEATR5A Homo sapiens HEAT repeat containing 5A (HEATR5A), mRNA [NM_015473] 2.69 5.52E-09 A_33_P3256303 SELPLG Homo sapiens selectin P ligand (SELPLG), mRNA [NM_003006] 2.69 1.00E-05 A_24_P319113 P2RX7 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, mRNA 2.68 4.50E-07 [NM_002562] A_24_P396994 ATP6V1A Homo sapiens ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A), mRNA [NM_001690] 2.67 1.30E-16

A_23_P2884 PIGH Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class H (PIGH), mRNA [NM_004569] 2.67 4.96E-09

A_33_P3355208 CROT Homo sapiens carnitine O-octanoyltransferase (CROT), transcript variant 3, non-coding RNA [NR_026585] 2.66 2.00E-05

A_24_P169343 C9orf21 Homo sapiens chromosome 9 open reading frame 21 (C9orf21), mRNA [NM_153698] 2.66 5.13E-07 A_33_P3347707 NOVA2 Homo sapiens neuro-oncological ventral antigen 2 (NOVA2), mRNA [NM_002516] 2.62 5.32E-03 A_33_P3263432 ITGA10 Homo sapiens integrin, alpha 10 (ITGA10), mRNA [NM_003637] 2.61 1.01E-08 A_32_P44775 C9orf85 Homo sapiens chromosome 9 open reading frame 85 (C9orf85), mRNA [NM_182505] 2.61 8.43E-09 A_33_P3258452 C12orf5 Homo sapiens chromosome 12 open reading frame 5 (C12orf5), mRNA [NM_020375] 2.60 7.67E-16 A_32_P48466 LCA5L Homo sapiens Leber congenital amaurosis 5-like (LCA5L), mRNA [NM_152505] 2.60 2.60E-04 A_33_P3320062 PLD1 Homo sapiens phospholipase D1, phosphatidylcholine-specific (PLD1), transcript variant 1, mRNA 2.60 6.00E-06 [NM_002662] A_24_P320665 FAM126A Homo sapiens family with sequence similarity 126, member A (FAM126A), mRNA [NM_032581] 2.59 5.92E-07 A_33_P3408918 SAA2 Homo sapiens serum amyloid A2 (SAA2), transcript variant 1, mRNA [NM_030754] 2.58 3.90E-03 A_23_P137532 PLOD1 Homo sapiens procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mRNA [NM_000302] 2.58 7.16E-16

A_23_P30254 PLK2 Homo sapiens polo-like kinase 2 (PLK2), mRNA [NM_006622] 2.58 7.68E-16 A_23_P137016 SAT1 Homo sapiens spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1, mRNA 2.58 7.22E-16 [NM_002970] A_33_P3282840 RPS29 Homo sapiens ribosomal protein S29 (RPS29), transcript variant 2, mRNA [NM_001030001] 2.57 1.22E-08 A_23_P37778 FHOD1 Homo sapiens formin homology 2 domain containing 1 (FHOD1), mRNA [NM_013241] 2.56 1.05E-15 A_24_P394368 WDR19 Homo sapiens WD repeat domain 19 (WDR19), mRNA [NM_025132] 2.55 1.85E-08 A_23_P166686 AMOTL2 Homo sapiens angiomotin like 2 (AMOTL2), mRNA [NM_016201] 2.55 2.47E-07 A_23_P258944 DNAJB9 Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9), mRNA [NM_012328] 2.54 1.66E-08 A_33_P3226542 SNORD3B-1 Homo sapiens small nucleolar RNA, C/D box 3B-1 (SNORD3B-1), small nucleolar RNA [NR_003271] 2.54 1.66E-08

10 Bastian et al. 2012

A_23_P255653 TNFRSF10A Homo sapiens tumor necrosis factor receptor superfamily, member 10a (TNFRSF10A), mRNA 2.54 8.40E-13 [NM_003844] A_33_P3462960 DNAJC3 Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 3 (DNAJC3), mRNA [NM_006260] 2.54 1.67E-15

A_33_P3214339 PLCXD2 Homo sapiens phosphatidylinositol-specific phospholipase C, X domain containing 2 (PLCXD2), transcript 2.53 3.50E-06 variant 2, mRNA [NM_153268] A_23_P214080 EGR1 Homo sapiens early growth response 1 (EGR1), mRNA [NM_001964] 2.53 1.23E-11 A_33_P3310189 ADRB1 Homo sapiens adrenergic, beta-1-, receptor (ADRB1), mRNA [NM_000684] 2.52 1.54E-14 A_23_P101476 ZNF442 Homo sapiens zinc finger protein 442 (ZNF442), mRNA [NM_030824] 2.52 0.00E+00 A_23_P58647 CTNNA1 Homo sapiens catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), mRNA [NM_001903] 2.52 2.35E-15

A_33_P3396089 SLC38A6 Homo sapiens solute carrier family 38, member 6 (SLC38A6), transcript variant 2, mRNA [NM_153811] 2.52 4.00E-05

A_32_P235796 BBS12 Homo sapiens Bardet-Biedl syndrome 12 (BBS12), transcript variant 2, mRNA [NM_152618] 2.51 2.41E-08 A_24_P251734 FSTL5 Homo sapiens follistatin-like 5 (FSTL5), transcript variant 1, mRNA [NM_020116] 2.51 6.50E-04 A_23_P66948 FAM59A Homo sapiens family with sequence similarity 59, member A (FAM59A), mRNA [NM_022751] 2.51 2.48E-08 A_24_P273799 ZNF641 Homo sapiens zinc finger protein 641 (ZNF641), transcript variant 1, mRNA [NM_152320] 2.51 8.28E-07 A_23_P327519 STARD4 Homo sapiens StAR-related lipid transfer (START) domain containing 4 (STARD4), mRNA [NM_139164] 2.50 3.07E-08

A_23_P55127 C17orf48 Homo sapiens chromosome 17 open reading frame 48 (C17orf48), mRNA [NM_020233] 2.50 2.70E-08 A_23_P132915 FAM114A1 Homo sapiens family with sequence similarity 114, member A1 (FAM114A1), transcript variant 1, mRNA 2.49 6.00E-05 [NM_138389] A_33_P3407925 TMEM49 Homo sapiens transmembrane protein 49 (TMEM49), mRNA [NM_030938] 2.49 2.76E-08 A_23_P421054 C6orf165 Homo sapiens chromosome 6 open reading frame 165 (C6orf165), mRNA [NM_001031743] 2.49 3.68E-03 A_23_P12554 PKD2L1 Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1), mRNA [NM_016112] 2.48 3.00E-04 A_23_P8281 IFNGR1 Homo sapiens interferon gamma receptor 1 (IFNGR1), mRNA [NM_000416] 2.47 6.22E-15 A_23_P107587 NPC1 Homo sapiens Niemann-Pick disease, type C1 (NPC1), mRNA [NM_000271] 2.46 0.00E+00 A_23_P202860 CYP2R1 Homo sapiens cytochrome P450, family 2, subfamily R, polypeptide 1 (CYP2R1), mRNA [NM_024514] 2.46 7.16E-15

A_33_P3252719 TIRAP Homo sapiens toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP), transcript variant 2.45 3.20E-04 3, mRNA [NM_001039661] A_33_P3539345 MYO6 Homo sapiens myosin VI (MYO6), mRNA [NM_004999] 2.45 2.37E-09 A_24_P303524 MICALL2 Homo sapiens MICAL-like 2 (MICALL2), transcript variant 1, mRNA [NM_182924] 2.45 4.12E-08 A_33_P3400374 PRIC285 Homo sapiens peroxisomal proliferator-activated receptor A interacting complex 285 (PRIC285), transcript 2.45 4.29E-08 variant 1, mRNA [NM_001037335] A_23_P129486 SEPX1 Homo sapiens selenoprotein X, 1 (SEPX1), mRNA [NM_016332] 2.45 4.25E-08 A_24_P381199 TRIM6 Homo sapiens tripartite motif-containing 6 (TRIM6), transcript variant 1, mRNA [NM_001003818] 2.44 4.62E-08 A_23_P153529 TRPM4 Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript 2.44 1.14E-14 variant 1, mRNA [NM_017636] A_23_P26854 ARHGAP44 Homo sapiens Rho GTPase activating protein 44 (ARHGAP44), mRNA [NM_014859] 2.44 4.75E-08 A_33_P3215953 MPZL1 Homo sapiens myelin protein zero-like 1 (MPZL1), transcript variant 2, mRNA [NM_024569] 2.44 1.18E-14 A_33_P3311439 GCH1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA [NM_001024071] 2.43 5.31E-13 A_33_P3777584 SERAC1 Homo sapiens serine active site containing 1 (SERAC1), mRNA [NM_032861] 2.43 2.08E-07 A_23_P410859 ZSWIM6 Homo sapiens zinc finger, SWIM-type containing 6 (ZSWIM6), mRNA [NM_020928] 2.43 5.26E-08 A_23_P5983 PLTP Homo sapiens phospholipid transfer protein (PLTP), transcript variant 1, mRNA [NM_006227] 2.43 2.72E-14 A_24_P236091 ENO2 Homo sapiens enolase 2 (gamma, neuronal) (ENO2), mRNA [NM_001975] 2.43 1.42E-14 A_33_P3296205 TSC22D1 Homo sapiens TSC22 domain family, member 1 (TSC22D1), transcript variant 1, mRNA [NM_183422] 2.43 1.44E-14

A_23_P114689 ASAP3 Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1, 2.42 0.00E+00 mRNA [NM_017707] A_33_P3379061 C9orf47 Homo sapiens chromosome 9 open reading frame 47 (C9orf47), transcript variant 1, mRNA 2.42 1.05E-07 [NM_001001938] A_33_P3419594 PDE9A Homo sapiens cDNA FLJ90181 fis, clone MAMMA1000706. [AK074662] 2.42 5.69E-08 A_23_P328074 SALL1 Homo sapiens sal-like 1 (Drosophila) (SALL1), transcript variant 1, mRNA [NM_002968] 2.42 3.41E-08 A_24_P406334 STEAP1 Homo sapiens six transmembrane epithelial antigen of the prostate 1 (STEAP1), mRNA [NM_012449] 2.42 4.90E-04

A_23_P368718 C13orf1 Homo sapiens chromosome 13 open reading frame 1 (C13orf1), transcript variant 1, mRNA [NM_020456] 2.42 6.97E-08

A_33_P3398862 RHOB Homo sapiens ras homolog gene family, member B (RHOB), mRNA [NM_004040] 2.42 1.08E-07 A_32_P14610 PDLIM5 Homo sapiens PDZ and LIM domain 5 (PDLIM5), transcript variant 1, mRNA [NM_006457] 2.42 1.93E-14 A_23_P217958 HK1 Homo sapiens hexokinase 1 (HK1), nuclear gene encoding mitochondrial protein, transcript variant 5, 2.42 1.84E-14 mRNA [NM_033500] A_23_P84334 TMEM39A Homo sapiens transmembrane protein 39A (TMEM39A), mRNA [NM_018266] 2.42 6.27E-08 A_32_P181103 GGCX Homo sapiens gamma-glutamyl carboxylase (GGCX), transcript variant 1, mRNA [NM_000821] 2.42 2.10E-14 A_23_P217109 SLC31A2 Homo sapiens solute carrier family 31 (copper transporters), member 2 (SLC31A2), mRNA [NM_001860] 2.41 6.68E-08

A_33_P3366048 C20orf112 Homo sapiens chromosome 20 open reading frame 112 (C20orf112), mRNA [NM_080616] 2.41 3.99E-03 A_33_P3244951 PDE8A Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 1, mRNA [NM_002605] 2.41 2.57E-14 A_23_P312718 PNPLA8 Homo sapiens patatin-like phospholipase domain containing 8 (PNPLA8), mRNA [NM_015723] 2.41 2.36E-14 A_24_P95154 TUSC3 Homo sapiens tumor suppressor candidate 3 (TUSC3), transcript variant 2, mRNA [NM_178234] 2.40 2.44E-14

A_33_P3344406 MEF2B Homo sapiens myocyte enhancer factor 2B (MEF2B), mRNA [NM_001145785] 2.40 7.13E-08 A_23_P40453 CBR3 Homo sapiens carbonyl reductase 3 (CBR3), mRNA [NM_001236] 2.40 2.60E-14 A_23_P121956 THG1L Homo sapiens tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) (THG1L), mRNA [NM_017872] 2.40 0.00E+00

A_23_P126844 TNFRSF25 Homo sapiens tumor necrosis factor receptor superfamily, member 25 (TNFRSF25), transcript variant 1, 2.40 2.75E-14 mRNA [NM_148965] A_23_P169278 AGTPBP1 Homo sapiens ATP/GTP binding protein 1 (AGTPBP1), mRNA [NM_015239] 2.40 7.57E-08

11 Bastian et al. 2012

A_33_P3358731 PCSK5 Homo sapiens proprotein convertase subtilisin/kexin type 5 (PCSK5), transcript variant 2, mRNA 2.40 3.20E-04 [NM_006200] A_23_P7791 OGFRL1 Homo sapiens opioid growth factor receptor-like 1 (OGFRL1), mRNA [NM_024576] 2.39 7.79E-08 A_33_P3289218 AAK1 Homo sapiens AP2 associated kinase 1 (AAK1), mRNA [NM_014911] 2.39 1.23E-07 A_33_P3331085 SEC24A Homo sapiens SEC24 family, member A (S. cerevisiae) (SEC24A), mRNA [NM_021982] 2.39 8.45E-08 A_24_P360763 CREM Homo sapiens cAMP responsive element modulator (CREM), transcript variant 2, mRNA [NM_001881] 2.39 4.66E-14

A_23_P217367 ATG4A Homo sapiens ATG4 autophagy related 4 homolog A (S. cerevisiae) (ATG4A), transcript variant 1, mRNA 2.38 9.00E-08 [NM_052936] A_23_P94319 KBTBD11 Homo sapiens kelch repeat and BTB (POZ) domain containing 11 (KBTBD11), mRNA [NM_014867] 2.38 9.46E-08

A_23_P9869 CNDP1 Homo sapiens carnosine dipeptidase 1 (metallopeptidase M20 family) (CNDP1), mRNA [NM_032649] 2.38 4.16E-03

A_24_P6517 PLEKHG1 Homo sapiens pleckstrin homology domain containing, family G (with RhoGef domain) member 1 2.37 7.89E-07 (PLEKHG1), mRNA [NM_001029884] A_33_P3297853 AKNA Homo sapiens AT-hook transcription factor (AKNA), mRNA [NM_030767] 2.37 5.20E-04 A_33_P3259587 SCAND2 Homo sapiens SCAN domain containing 2 pseudogene (SCAND2), transcript variant 2, non-coding RNA 2.37 5.00E-05 [NR_003654] A_23_P217528 KLF8 Homo sapiens Kruppel-like factor 8 (KLF8), transcript variant 1, mRNA [NM_007250] 2.36 6.20E-14 A_23_P149626 PLEKHG5 Homo sapiens pleckstrin homology domain containing, family G (with RhoGef domain) member 5 2.36 1.86E-06 (PLEKHG5), transcript variant 2, mRNA [NM_198681] A_32_P89310 PLEKHM3 Homo sapiens pleckstrin homology domain containing, family M, member 3 (PLEKHM3), mRNA 2.34 1.38E-07 [NM_001080475] A_23_P430658 HEYL Homo sapiens hairy/enhancer-of-split related with YRPW motif-like (HEYL), mRNA [NM_014571] 2.34 1.95E-07

A_23_P53162 STIM1 Homo sapiens stromal interaction molecule 1 (STIM1), mRNA [NM_003156] 2.34 9.96E-14 A_23_P42829 SND1 Homo sapiens staphylococcal nuclease and tudor domain containing 1 (SND1), mRNA [NM_014390] 2.34 9.64E-14

A_23_P200801 PDE4DIP Homo sapiens phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 5, mRNA 2.34 1.60E-07 [NM_001002811] A_33_P3217103 RBFOX2 Homo sapiens RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 1, mRNA 2.33 3.55E-07 [NM_001031695] A_33_P3315314 MT1H Homo sapiens metallothionein 1H (MT1H), mRNA [NM_005951] 2.33 1.56E-07 A_33_P3303572 EDA Homo sapiens ectodysplasin A (EDA), transcript variant 3, mRNA [NM_001005610] 2.33 2.43E-12 A_23_P129246 PLEKHO2 Homo sapiens pleckstrin homology domain containing, family O member 2 (PLEKHO2), transcript variant 1, 2.33 1.62E-07 mRNA [NM_025201] A_33_P3222367 SNORA74B Homo sapiens small nucleolar RNA, H/ACA box 74B (SNORA74B), small nucleolar RNA [NR_002988] 2.32 1.98E-07

A_23_P79221 ACVR1 Homo sapiens activin A receptor, type I (ACVR1), transcript variant 1, mRNA [NM_001105] 2.32 1.79E-13 A_23_P35456 SH3PXD2A Homo sapiens SH3 and PX domains 2A (SH3PXD2A), mRNA [NM_014631] 2.32 1.78E-07 A_23_P39402 ALKBH6 Homo sapiens alkB, alkylation repair homolog 6 (E. coli) (ALKBH6), transcript variant 1, mRNA 2.32 1.85E-07 [NM_198867] A_24_P649624 KIF1B Homo sapiens kinesin family member 1B (KIF1B), transcript variant 2, mRNA [NM_183416] 2.32 1.72E-13 A_23_P22350 GRAMD3 Homo sapiens GRAM domain containing 3 (GRAMD3), transcript variant 2, mRNA [NM_023927] 2.31 3.99E-07

A_23_P18493 PTPN13 Homo sapiens protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated 2.31 1.92E-13 phosphatase) (PTPN13), transcript variant 4, mRNA [NM_080685] A_32_P32739 NAGS Homo sapiens N-acetylglutamate synthase (NAGS), mRNA [NM_153006] 2.31 1.87E-13 A_33_P3394993 PREX2 Homo sapiens phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), 2.31 3.96E-03 transcript variant 2, mRNA [NM_025170] A_23_P422212 SLC35F3 Homo sapiens solute carrier family 35, member F3 (SLC35F3), mRNA [NM_173508] 2.31 2.30E-07 A_33_P3216467 FAM83E Homo sapiens family with sequence similarity 83, member E (FAM83E), mRNA [NM_017708] 2.31 2.75E-06 A_23_P415006 RAB11FIP5 Homo sapiens RAB11 family interacting protein 5 (class I) (RAB11FIP5), mRNA [NM_015470] 2.31 2.00E-13 A_23_P329198 OBFC2A Homo sapiens oligonucleotide/oligosaccharide-binding fold containing 2A (OBFC2A), transcript variant 1, 2.31 2.05E-07 mRNA [NM_001031716] A_33_P3382606 FBXW12 Homo sapiens F-box and WD repeat domain containing 12 (FBXW12), transcript variant 1, mRNA 2.31 6.50E-04 [NM_207102] A_33_P3263232 LRRC3 Homo sapiens leucine rich repeat containing 3 (LRRC3), mRNA [NM_030891] 2.30 2.33E-07 A_23_P151133 TSPAN9 Homo sapiens tetraspanin 9 (TSPAN9), transcript variant 1, mRNA [NM_006675] 2.30 2.24E-07 A_23_P12463 QSOX1 Homo sapiens quiescin Q6 sulfhydryl oxidase 1 (QSOX1), transcript variant 1, mRNA [NM_002826] 2.30 2.31E-13

A_23_P200239 YIPF1 Homo sapiens Yip1 domain family, member 1 (YIPF1), transcript variant 1, mRNA [NM_018982] 2.30 2.26E-07 A_33_P3220911 BST2 full-length cDNA clone CS0DL006YF17 of B cells (Ramos cell line) Cot 25-normalized of Homo sapiens 2.30 2.27E-07 (human). [CR591045] A_23_P46369 RAB13 Homo sapiens RAB13, member RAS oncogene family (RAB13), mRNA [NM_002870] 2.29 2.52E-07 A_23_P84666 GDPD1 Homo sapiens glycerophosphodiester phosphodiesterase domain containing 1 (GDPD1), transcript variant 2.29 9.46E-07 1, mRNA [NM_182569] A_24_P382489 SLC27A1 Homo sapiens solute carrier family 27 (fatty acid transporter), member 1 (SLC27A1), mRNA [NM_198580] 2.28 3.22E-13

A_23_P204550 SCYL2 Homo sapiens SCY1-like 2 (S. cerevisiae) (SCYL2), mRNA [NM_017988] 2.28 2.59E-07 A_23_P215525 OSBPL3 Homo sapiens oxysterol binding protein-like 3 (OSBPL3), transcript variant 1, mRNA [NM_015550] 2.28 3.80E-13

A_33_P3395738 BCAR1 Homo sapiens cDNA FLJ45059 fis, clone BRAWH3023274. [AK127004] 2.28 4.24E-03 A_23_P211806 LRRFIP2 Homo sapiens leucine rich repeat (in FLII) interacting protein 2 (LRRFIP2), transcript variant 2, mRNA 2.28 2.74E-07 [NM_017724] A_23_P86411 MYO3A Homo sapiens myosin IIIA (MYO3A), mRNA [NM_017433] 2.28 2.18E-06 A_23_P14915 CSNK2A2 Homo sapiens casein kinase 2, alpha prime polypeptide (CSNK2A2), mRNA [NM_001896] 2.28 3.61E-13 A_23_P206960 SEC14L1 Homo sapiens SEC14-like 1 (S. cerevisiae) (SEC14L1), transcript variant 1, mRNA [NM_003003] 2.28 2.77E-07 A_33_P3243878 GOLGA6L9 Homo sapiens golgin A6 family-like 9 (GOLGA6L9), mRNA [NM_198181] 2.28 2.78E-07

12 Bastian et al. 2012

A_33_P3489737 NLN Homo sapiens neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial 2.28 3.85E-13 protein, mRNA [NM_020726] A_23_P212715 CBLB Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), mRNA 2.27 4.35E-13 [NM_170662] A_23_P317184 LRRFIP2 Homo sapiens leucine rich repeat (in FLII) interacting protein 2 (LRRFIP2), transcript variant 1, mRNA 2.27 3.21E-07 [NM_006309] A_24_P219114 SEL1L sel-1 suppressor of lin-12-like (C. elegans) [Source:HGNC Symbol;Acc:10717] [ENST00000336735] 2.27 2.28E-09

A_23_P135248 CCL27 Homo sapiens chemokine (C-C motif) ligand 27 (CCL27), mRNA [NM_006664] 2.27 7.60E-03 A_32_P229746 DNAJB6 Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 6 (DNAJB6), transcript variant 2, mRNA 2.26 5.30E-13 [NM_005494] A_33_P3348220 ENGASE Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA [NM_001042573] 2.26 4.00E-05 A_24_P712562 C17orf67 Homo sapiens chromosome 17 open reading frame 67 (C17orf67), mRNA [NM_001085430] 2.26 2.00E-05 A_23_P39766 GLS Homo sapiens glutaminase (GLS), nuclear gene encoding mitochondrial protein, mRNA [NM_014905] 2.26 3.43E-07

A_23_P117082 HEBP1 Homo sapiens heme binding protein 1 (HEBP1), mRNA [NM_015987] 2.26 0.00E+00 A_23_P422981 FBXO36 Homo sapiens F-box protein 36 (FBXO36), mRNA [NM_174899] 2.26 4.38E-07 A_23_P382775 BBC3 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA [NM_014417] 2.26 6.19E-13 A_33_P3465247 KIF3A Homo sapiens kinesin family member 3A (KIF3A), mRNA [NM_007054] 2.26 6.28E-13 A_33_P3258660 SCD5 Homo sapiens stearoyl-CoA desaturase 5 (SCD5), transcript variant 2, mRNA [NM_024906] 2.25 5.72E-07 A_33_P3221665 KIF21B Homo sapiens kinesin family member 21B (KIF21B), mRNA [NM_017596] 2.25 1.06E-03 A_23_P255444 DAPP1 Homo sapiens dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mRNA [NM_014395] 2.25 4.98E-10

A_24_P269814 PLEKHA1 Homo sapiens pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2.25 5.46E-09 1 (PLEKHA1), transcript variant 2, mRNA [NM_001001974] A_33_P3392525 ARL4D Homo sapiens ADP-ribosylation factor-like 4D (ARL4D), mRNA [NM_001661] 2.25 4.35E-07 A_24_P141332 CAMK2G Homo sapiens calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 1, 2.25 7.88E-13 mRNA [NM_172171] A_23_P14184 THSD1 Homo sapiens thrombospondin, type I, domain containing 1 (THSD1), transcript variant 1, mRNA 2.25 4.04E-07 [NM_018676] A_23_P30069 DDX60L Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 60-like (DDX60L), mRNA [NM_001012967] 2.24 6.05E-07

A_23_P200126 MKNK1 Homo sapiens MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1, mRNA 2.24 8.30E-13 [NM_003684] A_33_P3312601 AADACL4 Homo sapiens arylacetamide deacetylase-like 4 (AADACL4), mRNA [NM_001013630] 2.24 1.00E-05 A_24_P109633 ITPK1 Homo sapiens inositol 1,3,4-triphosphate 5/6 kinase (ITPK1), transcript variant 3, mRNA [NM_001142594] 2.24 5.50E-11

A_24_P339869 ZNF295 Homo sapiens zinc finger protein 295 (ZNF295), transcript variant 2, mRNA [NM_020727] 2.24 4.58E-07 A_24_P202567 ITPKC Homo sapiens inositol 1,4,5-trisphosphate 3-kinase C (ITPKC), mRNA [NM_025194] 2.23 4.63E-07 A_23_P158725 SLC16A3 Homo sapiens solute carrier family 16, member 3 (monocarboxylic acid transporter 4) (SLC16A3), transcript 2.23 7.65E-07 variant 1, mRNA [NM_001042422] A_24_P225878 KIF9 Homo sapiens kinesin family member 9 (KIF9), transcript variant 1, mRNA [NM_022342] 2.23 5.73E-03 A_33_P3236646 MIA3 Homo sapiens melanoma inhibitory activity family, member 3 (MIA3), mRNA [NM_198551] 2.23 4.86E-07 A_23_P170857 IL1RAP Homo sapiens interleukin 1 receptor accessory protein (IL1RAP), transcript variant 1, mRNA [NM_002182] 2.23 1.19E-12

A_33_P3254634 PDIA5 Homo sapiens protein disulfide isomerase family A, member 5 (PDIA5), transcript variant 1, mRNA 2.23 1.69E-06 [NM_006810] A_33_P3418597 GAS2L1 Homo sapiens growth arrest-specific 2 like 1 (GAS2L1), transcript variant 3, mRNA [NM_152237] 2.23 7.00E-05 A_23_P398491 MRPL42P5 Homo sapiens mitochondrial ribosomal protein L42 pseudogene 5 (MRPL42P5), non-coding RNA 2.23 5.46E-07 [NR_002208] A_23_P111995 LOXL2 Homo sapiens lysyl oxidase-like 2 (LOXL2), mRNA [NM_002318] 2.22 0.00E+00 A_23_P201279 UBE4B Homo sapiens ubiquitination factor E4B (UFD2 homolog, yeast) (UBE4B), transcript variant 2, mRNA 2.22 1.27E-12 [NM_006048] A_33_P3284394 CBR1 Homo sapiens cDNA, FLJ18261. [AK311219] 2.22 2.00E-05 A_33_P3399935 RPS6KA2 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 2 (RPS6KA2), transcript variant 1, mRNA 2.22 4.32E-03 [NM_021135] A_23_P5831 HPCAL1 Homo sapiens hippocalcin-like 1 (HPCAL1), transcript variant 2, mRNA [NM_134421] 2.22 1.33E-12 A_23_P218434 ZNF226 Homo sapiens zinc finger protein 226 (ZNF226), transcript variant 3, mRNA [NM_001032374] 2.22 5.85E-07 A_32_P194246 CLEC16A Homo sapiens C-type lectin domain family 16, member A (CLEC16A), mRNA [NM_015226] 2.21 1.56E-12 A_33_P3328814 SETBP1 Homo sapiens SET binding protein 1 (SETBP1), transcript variant 2, mRNA [NM_001130110] 2.21 3.53E-03 A_23_P114626 SERPINC1 Homo sapiens serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1), mRNA 2.21 0.00E+00 [NM_000488] A_23_P12884 GRK5 Homo sapiens G protein-coupled receptor kinase 5 (GRK5), mRNA [NM_005308] 2.21 1.78E-12 A_33_P3222977 TEKT5 Homo sapiens tektin 5 (TEKT5), mRNA [NM_144674] 2.21 1.19E-06 A_33_P3390758 HSPA8 Homo sapiens heat shock 70kDa protein 8 (HSPA8), transcript variant 2, mRNA [NM_153201] 2.21 1.89E-12 A_23_P406330 SMAP2 Homo sapiens small ArfGAP2 (SMAP2), transcript variant 1, mRNA [NM_022733] 2.21 6.43E-07 A_23_P134113 C6orf192 Homo sapiens chromosome 6 open reading frame 192 (C6orf192), mRNA [NM_052831] 2.20 6.63E-07 A_33_P3311205 PRPF40B Homo sapiens PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae) (PRPF40B), transcript 2.20 1.00E-04 variant 1, mRNA [NM_001031698] A_24_P87114 USP30 Homo sapiens ubiquitin specific peptidase 30 (USP30), mRNA [NM_032663] 2.20 1.11E-06 A_23_P356965 CCDC57 Homo sapiens cDNA FLJ43953 fis, clone TESTI4015477. [AK125941] 2.20 8.70E-07 A_23_P97700 TXNIP Homo sapiens thioredoxin interacting protein (TXNIP), mRNA [NM_006472] 2.20 2.38E-12 A_23_P327698 LMBRD2 Homo sapiens LMBR1 domain containing 2 (LMBRD2), mRNA [NM_001007527] 2.20 1.00E-05 A_33_P3272090 PDE4DIP Homo sapiens phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 9, mRNA 2.19 2.22E-06 [NM_001198834] A_23_P431179 HIST1H4A Homo sapiens histone cluster 1, H4a (HIST1H4A), mRNA [NM_003538] 2.19 7.75E-07 A_23_P201764 DHDDS Homo sapiens dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1, mRNA [NM_024887] 2.19 1.52E-06

A_23_P29922 TLR3 Homo sapiens toll-like receptor 3 (TLR3), mRNA [NM_003265] 2.19 1.92E-11

13 Bastian et al. 2012

A_33_P3283669 ATP1A3 Homo sapiens ATPase, Na+/K+ transporting, alpha 3 polypeptide (ATP1A3), mRNA [NM_152296] 2.19 3.00E-12

A_23_P162037 ARNTL Homo sapiens aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 3, mRNA 2.19 3.13E-12 [NM_001030273] A_32_P36694 JAZF1 Homo sapiens JAZF zinc finger 1 (JAZF1), mRNA [NM_175061] 2.18 5.22E-12 A_23_P374389 PWWP2B Homo sapiens PWWP domain containing 2B (PWWP2B), transcript variant 1, mRNA [NM_138499] 2.17 9.31E-07

A_33_P3350056 MT1X Homo sapiens metallothionein 1X (MT1X), mRNA [NM_005952] 2.17 4.08E-12 A_33_P3266255 CSGALNACT2 Homo sapiens chondroitin sulfate N-acetylgalactosaminyltransferase 2 (CSGALNACT2), mRNA 2.17 1.00E-05 [NM_018590] A_24_P148836 KLHDC8B Homo sapiens kelch domain containing 8B (KLHDC8B), mRNA [NM_173546] 2.17 8.54E-06 A_33_P3318053 C17orf69 Homo sapiens chromosome 17 open reading frame 69 (C17orf69), transcript variant 2, non-coding RNA 2.17 7.74E-06 [NR_026906] A_33_P3378835 SLC9A3R1 Homo sapiens solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1), 2.17 4.50E-12 mRNA [NM_004252] A_23_P347468 FZD3 Homo sapiens frizzled homolog 3 (Drosophila) (FZD3), transcript variant 1, mRNA [NM_017412] 2.17 3.24E-11 A_24_P260443 THBS4 Homo sapiens thrombospondin 4 (THBS4), mRNA [NM_003248] 2.17 7.50E-04 A_33_P3327921 RHOQ Homo sapiens ras homolog gene family, member Q (RHOQ), mRNA [NM_012249] 2.16 3.85E-07 A_32_P20454 COPA Homo sapiens coatomer protein complex, subunit alpha (COPA), transcript variant 1, mRNA 2.16 5.15E-12 [NM_001098398] A_24_P216421 DTNB Homo sapiens dystrobrevin, beta (DTNB), transcript variant 3, mRNA [NM_033148] 2.16 2.74E-06 A_23_P90925 IL1F8 Homo sapiens interleukin 1 family, member 8 (eta) (IL1F8), transcript variant 2, mRNA [NM_173178] 2.16 3.64E-03

A_24_P36285 ZNF224 Homo sapiens zinc finger protein 224 (ZNF224), mRNA [NM_013398] 2.15 1.53E-06 A_33_P3363120 SCARNA10 Homo sapiens small Cajal body-specific RNA 10 (SCARNA10), guide RNA [NR_004387] 2.15 1.53E-06 A_33_P3349651 SWAP70 Homo sapiens SWAP switching B-cell complex 70kDa subunit (SWAP70), mRNA [NM_015055] 2.15 6.65E-12 A_33_P3748714 FUT10 Homo sapiens fucosyltransferase 10 (alpha (1,3) fucosyltransferase) (FUT10), mRNA [NM_032664] 2.15 1.51E-06

A_23_P316472 DNHD1 Homo sapiens dynein heavy chain domain 1 (DNHD1), transcript variant 1, mRNA [NM_144666] 2.15 1.57E-06 A_23_P39465 BST2 Homo sapiens bone marrow stromal cell antigen 2 (BST2), mRNA [NM_004335] 2.15 7.01E-12 A_23_P201368 CTBS Homo sapiens chitobiase, di-N-acetyl- (CTBS), mRNA [NM_004388] 2.15 1.29E-06 A_24_P245838 MGAT3 Homo sapiens mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (MGAT3), 2.14 2.00E-05 transcript variant 1, mRNA [NM_002409] A_33_P3341586 SIL1 Homo sapiens SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) (SIL1), transcript variant 1, 2.14 9.18E-12 mRNA [NM_001037633] A_23_P28246 SLC23A3 Homo sapiens solute carrier family 23 (nucleobase transporters), member 3 (SLC23A3), transcript variant 2.14 9.00E-05 1, mRNA [NM_144712] A_23_P97309 CASP9 Homo sapiens caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha, mRNA 2.14 8.90E-12 [NM_001229] A_24_P333019 RNF24 Homo sapiens ring finger protein 24 (RNF24), transcript variant 1, mRNA [NM_007219] 2.14 1.44E-06 A_23_P301247 HIST2H2AC Homo sapiens histone cluster 2, H2ac (HIST2H2AC), mRNA [NM_003517] 2.14 9.40E-12 A_23_P34942 EVI5 Homo sapiens ecotropic viral integration site 5 (EVI5), mRNA [NM_005665] 2.14 1.00E-04 A_23_P206724 MT1E Homo sapiens metallothionein 1E (MT1E), mRNA [NM_175617] 2.14 1.09E-11 A_33_P3808996 SPATS2 Homo sapiens spermatogenesis associated, serine-rich 2 (SPATS2), mRNA [NM_023071] 2.14 1.43E-06 A_33_P3410589 FAM43A Homo sapiens family with sequence similarity 43, member A (FAM43A), mRNA [NM_153690] 2.13 1.52E-06 A_23_P422083 TMEM55A Homo sapiens transmembrane protein 55A (TMEM55A), mRNA [NM_018710] 2.13 1.54E-06 A_24_P296698 MAP2K3 Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, mRNA 2.13 1.08E-11 [NM_145109] A_23_P121447 ARPM1 Homo sapiens actin related protein M1 (ARPM1), mRNA [NM_032487] 2.13 9.87E-12 A_23_P389897 NGFR Homo sapiens nerve growth factor receptor (NGFR), mRNA [NM_002507] 2.13 1.46E-11 A_33_P3316078 INTS6 Homo sapiens integrator complex subunit 6 (INTS6), transcript variant 3, mRNA [NM_001039938] 2.13 2.96E-10

A_23_P106682 EMP2 Homo sapiens epithelial membrane protein 2 (EMP2), mRNA [NM_001424] 2.13 0.00E+00 A_33_P3387796 PHLDB1 Homo sapiens pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant 1, mRNA 2.13 5.00E-05 [NM_015157] A_33_P3297126 TSNARE1 Homo sapiens t-SNARE domain containing 1 (TSNARE1), mRNA [NM_145003] 2.13 4.00E-05 A_23_P56673 INO80B Homo sapiens INO80 complex subunit B (INO80B), mRNA [NM_031288] 2.13 1.72E-06 A_24_P13230 RAB6A Homo sapiens RAB6A, member RAS oncogene family (RAB6A), transcript variant 1, mRNA [NM_002869] 2.13 1.21E-11

A_23_P168306 WASF1 Homo sapiens WAS protein family, member 1 (WASF1), transcript variant 1, mRNA [NM_003931] 2.12 1.26E-11 A_33_P3351735 CCDC87 Homo sapiens coiled-coil domain containing 87 (CCDC87), mRNA [NM_018219] 2.12 1.10E-04 A_33_P3351120 TXNRD1 Homo sapiens thioredoxin reductase 1 (TXNRD1), transcript variant 1, mRNA [NM_003330] 2.12 1.37E-11 A_33_P3494748 TMEM65 Homo sapiens transmembrane protein 65 (TMEM65), mRNA [NM_194291] 2.12 1.88E-06 A_23_P8906 LRP12 Homo sapiens low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 1, mRNA 2.12 2.15E-06 [NM_013437] A_33_P3317850 RIOK3 Homo sapiens RIO kinase 3 (yeast) (RIOK3), mRNA [NM_003831] 2.12 1.75E-06 A_24_P331830 KAZ Homo sapiens kazrin (KAZ), transcript variant A, mRNA [NM_015209] 2.12 2.84E-03 A_33_P3239736 WDR44 Homo sapiens WD repeat domain 44 (WDR44), transcript variant 1, mRNA [NM_019045] 2.12 2.43E-06 A_24_P918843 RALGAPA1 Homo sapiens Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2, 2.11 6.00E-05 mRNA [NM_194301] A_33_P3375314 ATP9A Homo sapiens ATPase, class II, type 9A (ATP9A), mRNA [NM_006045] 2.11 8.70E-06 A_24_P109432 NBEAL1 Homo sapiens neurobeachin-like 1 (NBEAL1), mRNA [NM_001114132] 2.11 2.24E-06 A_23_P207319 MAP3K14 Homo sapiens mitogen-activated protein kinase kinase kinase 14 (MAP3K14), mRNA [NM_003954] 2.11 1.74E-11

A_23_P115022 TMEM125 Homo sapiens transmembrane protein 125 (TMEM125), mRNA [NM_144626] 2.11 2.00E-05 A_33_P3250028 EVI5L Homo sapiens ecotropic viral integration site 5-like (EVI5L), transcript variant 2, mRNA [NM_145245] 2.11 1.98E-06

A_33_P3314643 SPEF1 Homo sapiens sperm flagellar 1 (SPEF1), mRNA [NM_015417] 2.11 2.94E-06 A_33_P3273584 SCARNA2 Homo sapiens small Cajal body-specific RNA 2 (SCARNA2), guide RNA [NR_003023] 2.11 2.17E-06

14 Bastian et al. 2012

A_23_P324718 SYNJ1 Homo sapiens synaptojanin 1 (SYNJ1), transcript variant 2, mRNA [NM_203446] 2.11 2.34E-11 A_33_P3709317 SNORA28 Homo sapiens small nucleolar RNA, H/ACA box 28 (SNORA28), small nucleolar RNA [NR_002964] 2.11 2.30E-06

A_33_P3237096 INPP5F Homo sapiens inositol polyphosphate-5-phosphatase F (INPP5F), transcript variant 3, non-coding RNA 2.11 2.13E-06 [NR_003251] A_33_P3351566 ETNK1 Homo sapiens ethanolamine kinase 1 (ETNK1), transcript variant 2, mRNA [NM_001039481] 2.11 3.55E-06 A_24_P7629 RPL32P3 Homo sapiens ribosomal protein L32 pseudogene 3 (RPL32P3), non-coding RNA [NR_003111] 2.10 2.60E-04 A_23_P19164 TTC1 Homo sapiens tetratricopeptide repeat domain 1 (TTC1), mRNA [NM_003314] 2.10 2.16E-06 A_23_P9875 TESK2 Homo sapiens testis-specific kinase 2 (TESK2), mRNA [NM_007170] 2.10 2.33E-06 A_23_P364517 SELK Homo sapiens selenoprotein K (SELK), mRNA [NM_021237] 2.10 2.23E-06 A_23_P48029 CLEC4A Homo sapiens C-type lectin domain family 4, member A (CLEC4A), transcript variant 1, mRNA 2.10 1.12E-10 [NM_016184] A_24_P487736 CXorf23 Homo sapiens chromosome X open reading frame 23 (CXorf23), mRNA [NM_198279] 2.10 4.44E-06 A_33_P3401252 HSP90B3P Homo sapiens heat shock protein 90kDa beta (Grp94), member 3 (pseudogene) (HSP90B3P), non-coding 2.10 2.36E-06 RNA [NR_003130] A_23_P420196 SOCS1 Homo sapiens suppressor of cytokine signaling 1 (SOCS1), mRNA [NM_003745] 2.09 7.15E-11 A_33_P3342992 UBE2Q2P2 Homo sapiens ubiquitin-conjugating enzyme E2Q family member 2 pseudogene 2 (UBE2Q2P2), non- 2.09 5.29E-06 coding RNA [NR_004847] A_24_P201739 SH2B3 Homo sapiens SH2B adaptor protein 3 (SH2B3), mRNA [NM_005475] 2.09 2.81E-11 A_33_P3398912 SLC2A6 Homo sapiens solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript 2.09 2.52E-06 variant 1, mRNA [NM_017585] A_33_P3238920 INPP5F Homo sapiens inositol polyphosphate-5-phosphatase F (INPP5F), transcript variant 1, mRNA [NM_014937] 2.09 2.88E-06

A_23_P69310 CCRL2 Homo sapiens chemokine (C-C motif) receptor-like 2 (CCRL2), transcript variant 1, mRNA [NM_003965] 2.09 4.60E-06

A_23_P376488 TNF Homo sapiens tumor necrosis factor (TNF), mRNA [NM_000594] 2.09 3.04E-11 A_24_P23411 ARMCX3 Homo sapiens armadillo repeat containing, X-linked 3 (ARMCX3), transcript variant 1, mRNA [NM_016607] 2.09 2.62E-06

A_23_P133068 ANK2 Homo sapiens ankyrin 2, neuronal (ANK2), transcript variant 1, mRNA [NM_001148] 2.09 8.16E-09 A_24_P159648 BAIAP2 Homo sapiens BAI1-associated protein 2 (BAIAP2), transcript variant 3, mRNA [NM_006340] 2.09 1.92E-10 A_33_P3374563 SPATA7 Homo sapiens spermatogenesis associated 7 (SPATA7), transcript variant 1, mRNA [NM_018418] 2.09 2.65E-06

A_32_P184488 PHLDB3 Homo sapiens pleckstrin homology-like domain, family B, member 3 (PHLDB3), mRNA [NM_198850] 2.09 2.67E-06

A_32_P25050 RDH10 Homo sapiens retinol dehydrogenase 10 (all-trans) (RDH10), mRNA [NM_172037] 2.09 2.90E-06 A_23_P423695 MXD4 Homo sapiens MAX dimerization protein 4 (MXD4), mRNA [NM_006454] 2.08 2.82E-06 A_23_P94819 RPH3AL Homo sapiens rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 1, mRNA [NM_006987] 2.08 7.73E-08

A_23_P134100 ULBP3 Homo sapiens UL16 binding protein 3 (ULBP3), mRNA [NM_024518] 2.08 1.60E-04 A_32_P151933 FECH Homo sapiens ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 1, 2.08 1.36E-08 mRNA [NM_001012515] A_24_P184555 PXN Homo sapiens paxillin (PXN), transcript variant 2, mRNA [NM_002859] 2.08 3.54E-11 A_32_P176550 JMY Homo sapiens junction mediating and regulatory protein, p53 cofactor (JMY), mRNA [NM_152405] 2.08 3.16E-06

A_23_P121527 KLHL5 Homo sapiens kelch-like 5 (Drosophila) (KLHL5), transcript variant 1, mRNA [NM_015990] 2.08 1.26E-44 A_33_P3404989 HIST1H3H Homo sapiens histone cluster 1, H3h (HIST1H3H), mRNA [NM_003536] 2.08 3.14E-06 A_23_P429998 FOSB Homo sapiens FBJ murine osteosarcoma viral oncogene homolog B (FOSB), transcript variant 1, mRNA 2.08 1.32E-06 [NM_006732] A_23_P85240 TLR7 Homo sapiens toll-like receptor 7 (TLR7), mRNA [NM_016562] 2.08 3.30E-04 A_24_P681301 UBC Homo sapiens ubiquitin C (UBC), mRNA [NM_021009] 2.07 3.09E-06 A_33_P3413048 PLXNA3 Homo sapiens plexin A3 (PLXNA3), mRNA [NM_017514] 2.07 3.13E-06 A_33_P3416321 SLC17A5 Homo sapiens solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5), mRNA 2.07 4.74E-11 [NM_012434] A_23_P253464 FAM175A Homo sapiens family with sequence similarity 175, member A (FAM175A), mRNA [NM_139076] 2.07 0.00E+00 A_23_P146576 GBGT1 Homo sapiens globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (GBGT1), mRNA [NM_021996] 2.07 4.46E-06

A_33_P3293668 DENND4C Homo sapiens DENN/MADD domain containing 4C (DENND4C), mRNA [NM_017925] 2.07 3.43E-06 A_33_P3316928 PELI1 Homo sapiens pellino homolog 1 (Drosophila) (PELI1), mRNA [NM_020651] 2.06 3.46E-06 A_24_P72518 AHCYL2 Homo sapiens adenosylhomocysteinase-like 2 (AHCYL2), transcript variant 1, mRNA [NM_015328] 2.06 3.48E-06

A_23_P330461 TMC4 Homo sapiens transmembrane channel-like 4 (TMC4), transcript variant 2, mRNA [NM_144686] 2.06 6.31E-06 A_24_P136470 BTN2A3 Homo sapiens butyrophilin, subfamily 2, member A3 (BTN2A3), non-coding RNA [NR_027795] 2.06 4.07E-06 A_32_P46981 HSBP1L1 Homo sapiens heat shock factor binding protein 1-like 1 (HSBP1L1), mRNA [NM_001136180] 2.06 2.50E-04 A_23_P97860 LIPA Homo sapiens lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2, mRNA 2.06 6.02E-11 [NM_000235] A_32_P104478 FGD6 Homo sapiens FYVE, RhoGEF and PH domain containing 6 (FGD6), mRNA [NM_018351] 2.06 3.81E-06 A_33_P3247848 ZNF814 Homo sapiens zinc finger protein 814 (ZNF814), mRNA [NM_001144989] 2.06 4.80E-06 A_23_P75647 HYOU1 Homo sapiens hypoxia up-regulated 1 (HYOU1), transcript variant 1, mRNA [NM_006389] 2.06 6.83E-11 A_33_P3299319 RPL28 Homo sapiens ribosomal protein L28 (RPL28), transcript variant 5, mRNA [NM_001136137] 2.06 3.92E-06 A_23_P391275 RCAN3 Homo sapiens RCAN family member 3 (RCAN3), mRNA [NM_013441] 2.05 6.29E-06 A_24_P95029 TAX1BP1 Homo sapiens Tax1 (human T-cell leukemia virus type I) binding protein 1 (TAX1BP1), transcript variant 1, 2.05 6.94E-11 mRNA [NM_006024] A_24_P98411 HSPA5 Homo sapiens heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mRNA 2.05 7.36E-11 [NM_005347] A_23_P108948 MREG Homo sapiens melanoregulin (MREG), mRNA [NM_018000] 2.05 0.00E+00 A_23_P89310 EPN2 Homo sapiens epsin 2 (EPN2), transcript variant 2, mRNA [NM_014964] 2.05 4.08E-06 A_33_P3403254 ZNF878 Homo sapiens zinc finger protein 878 (ZNF878), mRNA [NM_001080404] 2.05 1.00E-05 A_33_P3214105 ATF3 Homo sapiens activating transcription factor 3 (ATF3), transcript variant 1, mRNA [NM_001674] 2.05 4.82E-06 A_23_P305938 ZNF75D Homo sapiens zinc finger protein 75D (ZNF75D), transcript variant 1, mRNA [NM_007131] 2.05 4.29E-06

15 Bastian et al. 2012

A_24_P55496 OSR2 Homo sapiens odd-skipped related 2 (Drosophila) (OSR2), transcript variant 2, mRNA [NM_053001] 2.05 6.84E-06

A_23_P70670 CD83 Homo sapiens CD83 molecule (CD83), transcript variant 1, mRNA [NM_004233] 2.05 7.85E-11 A_33_P3227904 SLC25A34 Homo sapiens solute carrier family 25, member 34 (SLC25A34), mRNA [NM_207348] 2.05 5.06E-06 A_33_P3340014 TRO Homo sapiens trophinin (TRO), transcript variant 3, mRNA [NM_016157] 2.05 4.58E-06 A_24_P314554 GOLGA2P2Y Homo sapiens golgin A2 pseudogene 2, Y-linked (GOLGA2P2Y), non-coding RNA [NR_001555] 2.05 4.91E-06 A_24_P104119 RHOF Homo sapiens ras homolog gene family, member F (in filopodia) (RHOF), mRNA [NM_019034] 2.04 5.09E-06 A_33_P3354569 GPD2 Homo sapiens glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding 2.04 1.04E-10 mitochondrial protein, transcript variant 1, mRNA [NM_001083112] A_33_P3504659 CASP10 Homo sapiens caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant D, mRNA 2.04 9.48E-11 [NM_032977] A_32_P199252 HSP90AA1 Homo sapiens heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript 2.04 4.74E-06 variant 1, mRNA [NM_001017963] A_24_P394533 NEU1 Homo sapiens sialidase 1 (lysosomal sialidase) (NEU1), mRNA [NM_000434] 2.04 9.88E-11 A_33_P3308914 CIB2 Homo sapiens calcium and integrin binding family member 2 (CIB2), mRNA [NM_006383] 2.04 4.94E-06 A_24_P407235 CRY1 Homo sapiens cryptochrome 1 (photolyase-like) (CRY1), mRNA [NM_004075] 2.04 1.06E-10 A_24_P154080 ECE1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA [NM_001397] 2.04 2.00E-05 A_33_P3272231 MFSD2A Homo sapiens major facilitator superfamily domain containing 2A (MFSD2A), transcript variant 1, mRNA 2.04 4.92E-06 [NM_001136493] A_33_P3237207 C2orf72 Homo sapiens chromosome 2 open reading frame 72 (C2orf72), mRNA [NM_001144994] 2.04 1.00E-04 A_33_P3341234 UBE2H Homo sapiens ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) (UBE2H), transcript variant 1, 2.03 1.09E-10 mRNA [NM_003344] A_33_P3223923 PDIA3 Homo sapiens protein disulfide isomerase family A, member 3 (PDIA3), mRNA [NM_005313] 2.03 1.30E-10 A_23_P11843 LRRN2 Homo sapiens leucine rich repeat neuronal 2 (LRRN2), transcript variant 2, mRNA [NM_201630] 2.03 9.44E-39 A_23_P388871 HIST4H4 Homo sapiens histone cluster 4, H4 (HIST4H4), mRNA [NM_175054] 2.03 1.31E-08 A_23_P83351 DFNB31 Homo sapiens deafness, autosomal recessive 31 (DFNB31), transcript variant 1, mRNA [NM_015404] 2.03 1.52E-09

A_23_P92499 TLR2 Homo sapiens toll-like receptor 2 (TLR2), mRNA [NM_003264] 2.03 4.20E-45 A_23_P73835 MOSPD1 Homo sapiens motile sperm domain containing 1 (MOSPD1), mRNA [NM_019556] 2.03 5.64E-06 A_23_P314760 PRKAG2 Homo sapiens protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant 2.03 1.43E-10 a, mRNA [NM_016203] A_23_P254888 ZYX Homo sapiens zyxin (ZYX), transcript variant 1, mRNA [NM_003461] 2.03 1.28E-10 A_24_P7202 KIDINS220 Homo sapiens kinase D-interacting substrate, 220kDa (KIDINS220), mRNA [NM_020738] 2.03 6.62E-06 A_24_P942694 C10orf118 Homo sapiens chromosome 10 open reading frame 118 (C10orf118), mRNA [NM_018017] 2.03 5.92E-06 A_23_P148047 PTGER4 Homo sapiens prostaglandin E receptor 4 (subtype EP4) (PTGER4), mRNA [NM_000958] 2.02 1.40E-10 A_23_P200489 TMEM63A Homo sapiens transmembrane protein 63A (TMEM63A), mRNA [NM_014698] 2.02 6.75E-06 A_24_P218265 TNFRSF10B Homo sapiens tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), transcript variant 1, 2.02 1.50E-10 mRNA [NM_003842] A_32_P98975 C15orf57 Homo sapiens chromosome 15 open reading frame 57 (C15orf57), transcript variant 2, mRNA 2.02 6.19E-06 [NM_052849] A_23_P35467 CISD1 Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA [NM_018464] 2.02 1.66E-10 A_24_P54178 TMED5 Homo sapiens transmembrane emp24 protein transport domain containing 5 (TMED5), transcript variant 1, 2.02 6.31E-06 mRNA [NM_016040] A_23_P2601 HSP90B1 Homo sapiens heat shock protein 90kDa beta (Grp94), member 1 (HSP90B1), mRNA [NM_003299] 2.02 1.77E-10

A_23_P317756 ACSM3 Homo sapiens acyl-CoA synthetase medium-chain family member 3 (ACSM3), transcript variant 2, mRNA 2.01 1.03E-08 [NM_202000] A_33_P3340580 ZNF385C zinc finger protein 385C [Source:HGNC Symbol;Acc:33722] [ENST00000393873] 2.01 1.00E-04 A_23_P356109 ATXN7L1 Homo sapiens ataxin 7-like 1 (ATXN7L1), transcript variant 2, mRNA [NM_152749] 2.01 1.85E-03 A_23_P3368 FURIN Homo sapiens furin (paired basic amino acid cleaving enzyme) (FURIN), mRNA [NM_002569] 2.01 6.73E-09 A_24_P270460 IFI27 Homo sapiens interferon, alpha-inducible protein 27 (IFI27), transcript variant 2, mRNA [NM_005532] 2.01 3.10E-10

A_33_P3862375 USP45 Homo sapiens ubiquitin specific peptidase 45 (USP45), mRNA [NM_001080481] 2.01 1.00E-05 A_32_P103945 B4GALT4 Homo sapiens UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 (B4GALT4), transcript 2.01 7.39E-06 variant 1, mRNA [NM_212543] A_23_P250485 TRIM62 Homo sapiens tripartite motif-containing 62 (TRIM62), mRNA [NM_018207] 2.01 1.00E-05 A_33_P3289286 TMEM57 Homo sapiens transmembrane protein 57 (TMEM57), mRNA [NM_018202] 2.01 7.85E-06 A_33_P3365596 NAPB Homo sapiens N-ethylmaleimide-sensitive factor attachment protein, beta (NAPB), mRNA [NM_022080] 2.01 7.23E-06

A_23_P328206 DNMBP Homo sapiens dynamin binding protein (DNMBP), mRNA [NM_015221] 2.00 7.69E-06 A_23_P502170 DYNC2LI1 Homo sapiens dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2, mRNA 2.00 9.39E-06 [NM_015522] A_23_P34537 EPHX1 Homo sapiens epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), transcript variant 1, mRNA 2.00 2.89E-10 [NM_000120]

484 differentially upregulated genes after concomitant treatment with VPA and BTZ

are shown. Sequence code, Agilent Whole Human Genome Oligo Microarrays; Fold

Change, comparing concomitant treatment to untreated control; p - value, Two-way

ANOVA statistical analysis.

16 Bastian et al. 2012

Supplementary Table S8. Differential expression, fold change and statistical

analysis of the 435 genes downregulated in Reh cells only after concomitant

treatment with VPA and BTZ.

Sequence Primary Fold p-value Code Sequence Sequence Description [Accession number] Change Name

A_32_P34844 ADAMTS18 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 18 (ADAMTS18), mRNA -11.35 3.01E-11 [NM_199355] A_23_P137896 OXCT2 Homo sapiens 3-oxoacid CoA transferase 2 (OXCT2), mRNA [NM_022120] -8.30 1.00E-05 A_23_P47102 ACY3 Homo sapiens aspartoacylase (aminocyclase) 3 (ACY3), mRNA [NM_080658] -6.21 3.57E-12 A_33_P3323136 ENKUR Homo sapiens enkurin, TRPC channel interacting protein (ENKUR), mRNA [NM_145010] -5.59 3.00E-05 A_33_P3304065 AQP2 Homo sapiens aquaporin 2 (collecting duct) (AQP2), mRNA [NM_000486] -5.51 6.52E-19 A_32_P776626 RGPD1 Homo sapiens RANBP2-like and GRIP domain containing 1 (RGPD1), mRNA [NM_001024457] -5.49 8.00E-05 A_23_P41629 ADAMTS16 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16), mRNA -5.09 8.03E-03 [NM_139056] A_24_P228796 GAGE7 Homo sapiens G antigen 7 (GAGE7), mRNA [NM_021123] -5.06 9.71E-03 A_33_P3350913 TSEN2 Homo sapiens tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 5, -5.03 9.77E-03 mRNA [NM_001145395] A_32_P150142 DKFZp434L192 Homo sapiens hypothetical protein DKFZp434L192 (DKFZp434L192), non-coding RNA [NR_026929] -5.01 8.70E-03 A_33_P3391220 SULT1C2P1 Homo sapiens sulfotransferase family, cytosolic, 1C, member 2 pseudogene 1 (SULT1C2P1), non- -4.98 6.60E-04 coding RNA [NR_037191] A_33_P3249888 BEST3 Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA [NM_032735] -4.93 5.76E-15 A_23_P394986 CREG2 Homo sapiens cellular repressor of E1A-stimulated genes 2 (CREG2), mRNA [NM_153836] -4.65 3.37E-09 A_33_P3302448 LINGO3 Homo sapiens leucine rich repeat and Ig domain containing 3 (LINGO3), mRNA [NM_001101391] -4.56 7.14E-03 A_23_P147109 C9orf167 Homo sapiens chromosome 9 open reading frame 167 (C9orf167), mRNA [NM_017723] -4.48 9.25E-14 A_23_P81441 C5orf20 Homo sapiens chromosome 5 open reading frame 20 (C5orf20), mRNA [NM_130848] -4.43 5.83E-12 A_32_P33083 VCX2 Homo sapiens variable charge, X-linked 2 (VCX2), mRNA [NM_016378] -4.42 5.45E-10 A_33_P3390187 NHLRC4 Homo sapiens NHL repeat containing 4 (NHLRC4), mRNA [NM_176677] -4.18 4.38E-12 A_23_P17053 IL1F9 Homo sapiens interleukin 1 family, member 9 (IL1F9), mRNA [NM_019618] -4.11 4.25E-03 A_33_P3418662 HSD17B3 Homo sapiens hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3), mRNA [NM_000197] -4.11 4.84E-03 A_23_P120812 IGLL1 Homo sapiens immunoglobulin lambda-like polypeptide 1 (IGLL1), transcript variant 1, mRNA -4.08 2.28E-13 [NM_020070] A_23_P127186 TACC2 Homo sapiens transforming, acidic coiled-coil containing protein 2 (TACC2), transcript variant 1, mRNA -3.94 5.63E-24 [NM_206862] A_33_P3359027 CCDC42B Homo sapiens coiled-coil domain containing 42B (CCDC42B), mRNA [NM_001144872] -3.80 3.39E-11 A_24_P764690 ZNF720 full-length cDNA clone CS0DN002YO09 of Adult brain of Homo sapiens (human). [CR611323] -3.66 1.80E-04 A_23_P401904 PHF19 Homo sapiens PHD finger protein 19 (PHF19), transcript variant 2, mRNA [NM_001009936] -3.62 2.94E-12 A_33_P3209962 RASGRP2 Homo sapiens RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript -3.61 3.19E-12 variant 2, mRNA [NM_153819] A_23_P132079 C21orf62 Homo sapiens chromosome 21 open reading frame 62 (C21orf62), transcript variant 2, mRNA -3.61 5.75E-03 [NM_019596] A_33_P3341105 PDE6G Homo sapiens phosphodiesterase 6G, cGMP-specific, rod, gamma (PDE6G), transcript variant 1, -3.60 7.75E-23 mRNA [NM_002602] A_24_P274831 GIMAP7 Homo sapiens GTPase, IMAP family member 7 (GIMAP7), mRNA [NM_153236] -3.58 6.00E-05 A_23_P156739 C6orf125 Homo sapiens chromosome 6 open reading frame 125 (C6orf125), mRNA [NM_032340] -3.56 4.37E-12 A_32_P152767 C10orf140 Homo sapiens chromosome 10 open reading frame 140 (C10orf140), mRNA [NM_207371] -3.52 8.40E-07 A_23_P25674 CKB Homo sapiens creatine kinase, brain (CKB), mRNA [NM_001823] -3.50 2.84E-22 A_33_P3343106 ETV2 Homo sapiens ets variant 2 (ETV2), mRNA [NM_014209] -3.46 9.29E-12 A_33_P3243702 KLHL30 Homo sapiens kelch-like 30 (Drosophila) (KLHL30), mRNA [NM_198582] -3.33 2.67E-11 A_33_P3313145 ITIH3 Homo sapiens inter-alpha (globulin) inhibitor H3 (ITIH3), mRNA [NM_002217] -3.30 1.35E-20 A_24_P5743 ALDH16A1 Homo sapiens aldehyde dehydrogenase 16 family, member A1 (ALDH16A1), transcript variant 1, -3.29 2.92E-11 mRNA [NM_153329] A_32_P52018 PHACTR1 Homo sapiens phosphatase and actin regulator 1 (PHACTR1), mRNA [NM_030948] -3.27 3.20E-11 A_32_P40463 NUDT9P1 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 9 pseudogene 1 (NUDT9P1), -3.25 3.72E-10 non-coding RNA [NR_002779] A_23_P414978 NUDT14 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 14 (NUDT14), mRNA -3.22 2.96E-10 [NM_177533] A_33_P3271652 HHIPL1 Homo sapiens HHIP-like 1 (HHIPL1), transcript variant 2, mRNA [NM_032425] -3.17 3.14E-03 A_33_P3249509 NFIC Homo sapiens nuclear factor I/C (CCAAT-binding transcription factor) (NFIC), transcript variant 2, -3.17 6.92E-11 mRNA [NM_205843] A_33_P3353816 ITGA4 Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4), mRNA -3.14 3.96E-20 [NM_000885] A_23_P110851 TERT Homo sapiens telomerase reverse transcriptase (TERT), transcript variant 1, mRNA [NM_198253] -3.14 0.00E+00 A_23_P410625 ZNF367 Homo sapiens zinc finger protein 367 (ZNF367), mRNA [NM_153695] -3.13 7.69E-10 A_24_P195669 MYO15B Homo sapiens cDNA FLJ90199 fis, clone MAMMA1001609. [AK074680] -3.13 1.03E-10

17 Bastian et al. 2012

A_23_P79518 IL1B Homo sapiens interleukin 1, beta (IL1B), mRNA [NM_000576] -3.09 0.00E+00 A_23_P109229 SUN5 Homo sapiens Sad1 and UNC84 domain containing 5 (SUN5), mRNA [NM_080675] -3.05 2.03E-03 A_24_P626850 H6PD Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mRNA -3.05 8.90E-04 [NM_004285] A_33_P3307008 C13orf40 Homo sapiens chromosome 13 open reading frame 40 (C13orf40), mRNA [NM_001146197] -3.05 5.16E-03 A_23_P314789 ZDHHC19 Homo sapiens zinc finger, DHHC-type containing 19 (ZDHHC19), mRNA [NM_001039617] -3.02 3.60E-03 A_33_P3238976 TRMT5 Homo sapiens TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5), mRNA -3.02 2.11E-10 [NM_020810] A_32_P416583 NLRC5 Homo sapiens NLR family, CARD domain containing 5 (NLRC5), mRNA [NM_032206] -3.02 2.23E-10 A_33_P3548860 SEC1 Homo sapiens secretory blood group 1 (SEC1), non-coding RNA [NR_004401] -3.01 2.54E-08 A_24_P373844 KCTD15 Homo sapiens potassium channel tetramerisation domain containing 15 (KCTD15), transcript variant 1, -3.01 4.93E-10 mRNA [NM_024076] A_33_P3214670 HK2 Homo sapiens hexokinase 2 (HK2), mRNA [NM_000189] -3.01 8.15E-03 A_23_P67748 RAB11B Homo sapiens RAB11B, member RAS oncogene family (RAB11B), mRNA [NM_004218] -3.01 1.37E-18 A_33_P3239790 ZNF646 Homo sapiens zinc finger protein 646 (ZNF646), mRNA [NM_014699] -3.00 5.76E-06 A_32_P121085 DOK3 Homo sapiens docking protein 3 (DOK3), transcript variant 2, mRNA [NM_001144875] -2.99 2.72E-10 A_24_P305784 SPANXB2 Homo sapiens SPANX family, member B2 (SPANXB2), mRNA [NM_145664] -2.96 1.14E-09 A_23_P145238 HIST1H2BK Homo sapiens histone cluster 1, H2bk (HIST1H2BK), mRNA [NM_080593] -2.94 1.04E-18 A_33_P3382031 FAM23A Homo sapiens family with sequence similarity 23, member A (FAM23A), mRNA [NM_001098844] -2.92 4.70E-09 A_33_P3384392 ADRBK1 Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1), mRNA [NM_001619] -2.90 1.84E-18 A_33_P3236846 GPR17 Homo sapiens G protein-coupled receptor 17 (GPR17), transcript variant 1, mRNA [NM_001161415] -2.90 6.23E-03 A_24_P87036 ANO1 Homo sapiens anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1, mRNA -2.88 4.02E-17 [NM_018043] A_33_P3351101 TYSND1 Homo sapiens trypsin domain containing 1 (TYSND1), transcript variant 1, mRNA [NM_173555] -2.86 1.50E-09 A_24_P184388 LRTOMT Homo sapiens leucine rich transmembrane and 0-methyltransferase domain containing (LRTOMT), -2.85 9.75E-10 transcript variant 1, mRNA [NM_145309] A_23_P37359 CIDEB Homo sapiens cell death-inducing DFFA-like effector b (CIDEB), mRNA [NM_014430] -2.84 1.03E-08 A_23_P326474 L3MBTL4 Homo sapiens l(3)mbt-like 4 (Drosophila) (L3MBTL4), mRNA [NM_173464] -2.82 1.81E-07 A_24_P917819 ANKRD30BP2 Homo sapiens ankyrin repeat domain 30B pseudogene 2 (ANKRD30BP2), non-coding RNA -2.81 1.45E-09 [NR_026916] A_23_P102988 TXNRD2 Homo sapiens thioredoxin reductase 2 (TXNRD2), nuclear gene encoding mitochondrial protein, mRNA -2.80 4.81E-16 [NM_006440] A_23_P82588 C7orf55 Homo sapiens chromosome 7 open reading frame 55 (C7orf55), nuclear gene encoding mitochondrial -2.80 1.44E-09 protein, mRNA [NM_197964] A_23_P309361 C1orf59 Homo sapiens chromosome 1 open reading frame 59 (C1orf59), transcript variant 1, mRNA -2.79 1.48E-09 [NM_144584] A_23_P154234 POLE4 Homo sapiens polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), mRNA [NM_019896] -2.79 1.56E-09 A_33_P3403927 SAMD10 Homo sapiens sterile alpha motif domain containing 10 (SAMD10), mRNA [NM_080621] -2.78 1.77E-09 A_23_P98974 LGR5 Homo sapiens leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), mRNA -2.77 5.08E-16 [NM_003667] A_23_P202894 ACRV1 Homo sapiens acrosomal vesicle protein 1 (ACRV1), transcript variant 1, mRNA [NM_001612] -2.77 1.77E-03 A_23_P47208 BANF1 Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1, mRNA [NM_003860] -2.76 2.24E-17 A_23_P313828 CENPV Homo sapiens centromere protein V (CENPV), mRNA [NM_181716] -2.75 2.18E-09 A_23_P100583 LPO Homo sapiens lactoperoxidase (LPO), transcript variant 1, mRNA [NM_006151] -2.75 1.50E-04 A_24_P923381 EPR1 Homo sapiens effector cell peptidase receptor 1 (non-protein coding) (EPR1), non-coding RNA -2.75 9.35E-10 [NR_002219] A_23_P338505 C19orf40 Homo sapiens chromosome 19 open reading frame 40 (C19orf40), mRNA [NM_152266] -2.74 2.02E-08 A_23_P212617 TFRC Homo sapiens transferrin receptor (p90, CD71) (TFRC), mRNA [NM_003234] -2.73 0.00E+00 A_33_P3294222 RLTPR Homo sapiens RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing -2.72 2.95E-09 (RLTPR), mRNA [NM_001013838] A_33_P3386765 ABHD14A Homo sapiens abhydrolase domain containing 14A (ABHD14A), mRNA [NM_015407] -2.72 3.00E-09 A_23_P52738 DCPS Homo sapiens decapping enzyme, scavenger (DCPS), mRNA [NM_014026] -2.71 5.59E-17 A_23_P12866 PSTK Homo sapiens phosphoseryl-tRNA kinase (PSTK), mRNA [NM_153336] -2.71 4.08E-09 A_24_P393740 FYB Homo sapiens FYN binding protein (FYB), transcript variant 1, mRNA [NM_001465] -2.71 5.97E-17 A_23_P158277 TMCO4 Homo sapiens transmembrane and coiled-coil domains 4 (TMCO4), mRNA [NM_181719] -2.70 4.22E-09 A_33_P3230244 CSMD1 Homo sapiens CUB and Sushi multiple domains 1 (CSMD1), mRNA [NM_033225] -2.69 6.72E-08 A_23_P132175 RTN4R Homo sapiens reticulon 4 receptor (RTN4R), mRNA [NM_023004] -2.68 9.91E-17 A_33_P3418790 GNRH2 gonadotropin-releasing hormone 2 [Source:HGNC Symbol;Acc:4420] [ENST00000380347] -2.68 4.28E-03 A_33_P3253234 IQSEC2 Homo sapiens IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 3, non-coding RNA -2.66 5.60E-09 [NR_024449] A_33_P3232516 KIF18B Homo sapiens kinesin family member 18B (KIF18B), mRNA [NM_001080443] -2.66 3.00E-05 A_23_P139486 CDK2AP1 Homo sapiens cyclin-dependent kinase 2 associated protein 1 (CDK2AP1), mRNA [NM_004642] -2.66 1.51E-16 A_24_P244410 C11orf51 Homo sapiens chromosome 11 open reading frame 51 (C11orf51), mRNA [NM_014042] -2.65 5.52E-09 A_23_P360804 CPNE5 Homo sapiens copine V (CPNE5), mRNA [NM_020939] -2.65 6.23E-09 A_23_P410587 PHF17 Homo sapiens PHD finger protein 17 (PHF17), transcript variant S, mRNA [NM_024900] -2.64 6.31E-09 A_33_P3344209 ZDHHC11 Homo sapiens zinc finger, DHHC-type containing 11 (ZDHHC11), mRNA [NM_024786] -2.63 9.28E-03 A_33_P3237775 NR1H3 Homo sapiens nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1, mRNA -2.63 2.42E-16 [NM_005693] A_33_P3266410 MAZ Homo sapiens MYC-associated zinc finger protein (purine-binding transcription factor) (MAZ), transcript -2.63 9.02E-09 variant 1, mRNA [NM_002383] A_33_P3256054 MLST8 Homo sapiens MTOR associated protein, LST8 homolog (S. cerevisiae) (MLST8), transcript variant 1, -2.62 7.57E-09 mRNA [NM_022372] A_23_P427148 PROCA1 Homo sapiens protein interacting with cyclin A1 (PROCA1), mRNA [NM_152465] -2.62 7.85E-09 A_33_P3210492 C1QTNF4 Homo sapiens C1q and tumor necrosis factor related protein 4 (C1QTNF4), mRNA [NM_031909] -2.62 7.72E-09 A_23_P111672 TES Homo sapiens testis derived transcript (3 LIM domains) (TES), transcript variant 2, mRNA -2.61 7.60E-17 [NM_152829] A_23_P150238 C11orf68 Homo sapiens chromosome 11 open reading frame 68 (C11orf68), transcript variant 2, mRNA -2.61 8.25E-09 [NM_031450] A_24_P417706 MXD3 Homo sapiens MAX dimerization protein 3 (MXD3), transcript variant 2, mRNA [NM_001142935] -2.61 7.78E-16 A_24_P211151 EXOSC5 Homo sapiens exosome component 5 (EXOSC5), mRNA [NM_020158] -2.61 8.73E-09

18 Bastian et al. 2012

A_23_P112774 PTP4A3 Homo sapiens protein tyrosine phosphatase type IVA, member 3 (PTP4A3), transcript variant 1, mRNA -2.60 4.34E-16 [NM_032611] A_33_P3400273 SELL Homo sapiens selectin L (SELL), transcript variant 1, mRNA [NM_000655] -2.60 4.97E-16 A_33_P3256738 C9orf100 Homo sapiens chromosome 9 open reading frame 100 (C9orf100), mRNA [NM_032818] -2.60 7.28E-08 A_23_P217015 SET Homo sapiens SET nuclear oncogene (SET), transcript variant 2, mRNA [NM_003011] -2.60 9.60E-09 A_24_P244699 NUDT15 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15), mRNA -2.60 1.09E-08 [NM_018283] A_23_P80032 E2F1 Homo sapiens E2F transcription factor 1 (E2F1), mRNA [NM_005225] -2.59 0.00E+00 A_23_P256504 AMBP Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA [NM_001633] -2.59 9.44E-08 A_24_P169148 HMGB1 Homo sapiens high-mobility group box 1 (HMGB1), mRNA [NM_002128] -2.58 1.08E-08 A_23_P42265 APOM Homo sapiens apolipoprotein M (APOM), mRNA [NM_019101] -2.58 7.17E-16 A_33_P3243028 DSPP Homo sapiens dentin sialophosphoprotein (DSPP), mRNA [NM_014208] -2.58 3.42E-03 A_24_P239419 EPM2A Homo sapiens epilepsy, progressive myoclonus type 2A, Lafora disease (laforin) (EPM2A), transcript -2.57 1.09E-08 variant 2, mRNA [NM_001018041] A_33_P3236157 UNG Homo sapiens uracil-DNA glycosylase (UNG), nuclear gene encoding mitochondrial protein, transcript -2.56 1.15E-15 variant 1, mRNA [NM_003362] A_33_P3270197 DIS3L2 Homo sapiens DIS3 mitotic control homolog (S. cerevisiae)-like 2 (DIS3L2), mRNA [NM_152383] -2.56 3.60E-04 A_24_P237878 FAM195B Homo sapiens family with sequence similarity 195, member B (FAM195B), transcript variant 1, mRNA -2.55 1.46E-08 [NM_207368] A_33_P3391796 NOG Homo sapiens noggin (NOG), mRNA [NM_005450] -2.55 1.21E-15 A_33_P3295056 PTPRCAP Homo sapiens protein tyrosine phosphatase, receptor type, C-associated protein (PTPRCAP), mRNA -2.55 1.27E-15 [NM_005608] A_33_P3230399 ZNF784 Homo sapiens zinc finger protein 784 (ZNF784), mRNA [NM_203374] -2.54 1.63E-08 A_23_P160582 HYI Homo sapiens hydroxypyruvate isomerase (putative) (HYI), transcript variant 1, mRNA [NM_031207] -2.54 1.70E-08 A_23_P101742 MRPL54 Homo sapiens mitochondrial ribosomal protein L54 (MRPL54), nuclear gene encoding mitochondrial -2.54 1.71E-08 protein, mRNA [NM_172251] A_23_P28434 VAMP8 Homo sapiens vesicle-associated membrane protein 8 (endobrevin) (VAMP8), mRNA [NM_003761] -2.54 1.64E-15 A_23_P124122 PXMP2 Homo sapiens peroxisomal membrane protein 2, 22kDa (PXMP2), mRNA [NM_018663] -2.53 1.79E-08 A_33_P3375467 GPLD1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2, -2.53 6.75E-10 mRNA [NM_177483] A_23_P218412 SPAG7 Homo sapiens sperm associated antigen 7 (SPAG7), mRNA [NM_004890] -2.53 1.88E-08 A_33_P3220095 ZNF341 Homo sapiens zinc finger protein 341 (ZNF341), mRNA [NM_032819] -2.53 1.95E-08 A_33_P3253144 DOK3 Homo sapiens docking protein 3 (DOK3), transcript variant 1, mRNA [NM_024872] -2.53 1.90E-08 A_24_P156886 DDX59 Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 59 (DDX59), mRNA [NM_001031725] -2.53 2.78E-08 A_24_P171873 FBXO4 Homo sapiens F-box protein 4 (FBXO4), transcript variant 1, mRNA [NM_012176] -2.52 1.21E-07 A_32_P143000 FAM189A1 Homo sapiens family with sequence similarity 189, member A1 (FAM189A1), mRNA [NM_015307] -2.51 8.51E-08 A_23_P46378 PIGV Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class V (PIGV), mRNA [NM_017837] -2.51 2.36E-08 A_33_P3416797 OVOS2 Homo sapiens mRNA; cDNA DKFZp434C0631 (from clone DKFZp434C0631). [AL831947] -2.50 1.76E-07 A_33_P3266873 MSL1 Homo sapiens male-specific lethal 1 homolog (Drosophila) (MSL1), mRNA [NM_001012241] -2.50 2.50E-08 A_33_P3215517 RCC2 Homo sapiens regulator of chromosome condensation 2 (RCC2), transcript variant 1, mRNA -2.50 2.71E-08 [NM_018715] A_24_P232365 APBB1IP Homo sapiens amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein -2.50 2.59E-08 (APBB1IP), mRNA [NM_019043] A_33_P3276918 FAM64A Homo sapiens family with sequence similarity 64, member A (FAM64A), transcript variant 2, mRNA -2.50 2.65E-08 [NM_001195228] A_23_P325040 TMPO Homo sapiens thymopoietin (TMPO), transcript variant 1, mRNA [NM_003276] -2.50 3.94E-15 A_23_P142389 LSR Homo sapiens lipolysis stimulated lipoprotein receptor (LSR), transcript variant 2, mRNA [NM_205834] -2.49 3.14E-07 A_23_P72059 VSIG10 Homo sapiens V-set and immunoglobulin domain containing 10 (VSIG10), mRNA [NM_019086] -2.49 3.18E-08 A_24_P826348 ZC3H6 Homo sapiens zinc finger CCCH-type containing 6 (ZC3H6), mRNA [NM_198581] -2.48 4.93E-08 A_24_P210829 NME4 Homo sapiens non-metastatic cells 4, protein expressed in (NME4), nuclear gene encoding -2.48 3.24E-08 mitochondrial protein, mRNA [NM_005009] A_33_P3330099 ARSD Homo sapiens arylsulfatase D (ARSD), transcript variant 1, mRNA [NM_001669] -2.48 3.29E-08 A_33_P3313055 NOTCH3 Homo sapiens notch 3 (NOTCH3), mRNA [NM_000435] -2.47 1.00E-05 A_33_P3235053 TRIM47 Homo sapiens tripartite motif-containing 47 (TRIM47), mRNA [NM_033452] -2.47 3.61E-08 A_33_P3280965 SNHG9 Homo sapiens small nucleolar RNA host gene 9 (non-protein coding) (SNHG9), non-coding RNA -2.47 3.62E-08 [NR_003142] A_33_P3213857 RNASEH2B Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant 2, mRNA [NM_001142279] -2.46 3.73E-08 A_24_P364954 C17orf101 Homo sapiens chromosome 17 open reading frame 101 (C17orf101), transcript variant 1, mRNA -2.45 4.91E-08 [NM_024648] A_33_P3323323 ZNF497 Homo sapiens zinc finger protein 497 (ZNF497), mRNA [NM_198458] -2.45 4.33E-08 A_23_P143817 MYLK Homo sapiens myosin light chain kinase (MYLK), transcript variant 1, mRNA [NM_053025] -2.45 9.28E-15 A_24_P406301 NDUFB2 Homo sapiens NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa (NDUFB2), nuclear -2.45 4.46E-08 gene encoding mitochondrial protein, mRNA [NM_004546] A_23_P20823 WDR34 Homo sapiens WD repeat domain 34 (WDR34), mRNA [NM_052844] -2.44 4.77E-08 A_23_P36700 TAPBPL Homo sapiens TAP binding protein-like (TAPBPL), mRNA [NM_018009] -2.44 5.46E-08 A_32_P438767 NEUROG2 Homo sapiens neurogenin 2 (NEUROG2), mRNA [NM_024019] -2.44 8.41E-08 A_33_P3298775 PRSSL1 Homo sapiens protease, serine-like 1 (PRSSL1), mRNA [NM_214710] -2.43 5.39E-08 A_23_P413788 FUT11 Homo sapiens fucosyltransferase 11 (alpha (1,3) fucosyltransferase) (FUT11), mRNA [NM_173540] -2.43 7.35E-08 A_24_P238499 C18orf56 Homo sapiens chromosome 18 open reading frame 56 (C18orf56), mRNA [NM_001012716] -2.42 5.71E-08 A_23_P41664 LRRC70 Homo sapiens leucine rich repeat containing 70 (LRRC70), mRNA [NM_181506] -2.42 6.07E-08 A_23_P74269 SRM Homo sapiens spermidine synthase (SRM), mRNA [NM_003132] -2.42 1.72E-14 A_24_P237586 ANKRD37 Homo sapiens ankyrin repeat domain 37 (ANKRD37), mRNA [NM_181726] -2.42 6.02E-08 A_24_P141019 ST7 Homo sapiens suppression of tumorigenicity 7 (ST7), transcript variant a, mRNA [NM_018412] -2.42 2.70E-04 A_23_P41194 LRRC34 Homo sapiens leucine rich repeat containing 34 (LRRC34), transcript variant 3, mRNA [NM_153353] -2.41 6.57E-08 A_33_P3343073 SMARCB1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily -2.41 6.43E-08 b, member 1 (SMARCB1), transcript variant 1, mRNA [NM_003073] A_33_P3388870 BLOC1S1 Homo sapiens biogenesis of lysosomal organelles complex-1, subunit 1 (BLOC1S1), transcript variant -2.41 6.64E-08 1, mRNA [NM_001487] A_23_P360626 PLD6 Homo sapiens phospholipase D family, member 6 (PLD6), mRNA [NM_178836] -2.41 6.55E-08 A_23_P13701 TMBIM4 Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), mRNA [NM_016056] -2.41 6.73E-08

19 Bastian et al. 2012

A_32_P222383 HMGN2 Homo sapiens high-mobility group nucleosomal binding domain 2 (HMGN2), mRNA [NM_005517] -2.41 2.28E-14 A_23_P128375 C12orf34 Homo sapiens chromosome 12 open reading frame 34 (C12orf34), mRNA [NM_032829] -2.41 8.66E-08 A_23_P138849 KAT5 Homo sapiens K(lysine) acetyltransferase 5 (KAT5), transcript variant 2, mRNA [NM_006388] -2.40 2.92E-14 A_23_P58321 CCNA2 Homo sapiens cyclin A2 (CCNA2), mRNA [NM_001237] -2.40 0.00E+00 A_33_P3360157 OR5AS1 Homo sapiens olfactory receptor, family 5, subfamily AS, member 1 (OR5AS1), mRNA -2.39 4.40E-04 [NM_001001921] A_23_P310 MARCKSL1 Homo sapiens MARCKS-like 1 (MARCKSL1), mRNA [NM_023009] -2.39 8.36E-08 A_33_P3376116 SPC24 Homo sapiens SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24), -2.38 1.02E-07 mRNA [NM_182513] A_33_P3355185 BCL2 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript -2.38 3.85E-14 variant beta, mRNA [NM_000657] A_32_P453321 C1orf228 Homo sapiens chromosome 1 open reading frame 228 (C1orf228), mRNA [NM_001145636] -2.38 8.89E-08 A_32_P60223 ING5 Homo sapiens inhibitor of growth family, member 5 (ING5), mRNA [NM_032329] -2.38 9.07E-08 A_23_P83192 PHPT1 Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mRNA [NM_014172] -2.38 4.11E-14 A_33_P3372451 C6orf108 Homo sapiens chromosome 6 open reading frame 108 (C6orf108), transcript variant 2, mRNA -2.37 1.02E-07 [NM_199184] A_24_P83586 MMACHC Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria -2.37 5.73E-14 (MMACHC), mRNA [NM_015506] A_32_P161913 C1orf70 Homo sapiens chromosome 1 open reading frame 70 (C1orf70), mRNA [NM_001114748] -2.36 1.66E-07 A_23_P171388 TMSB4Y Homo sapiens thymosin beta 4, Y-linked (TMSB4Y), mRNA [NM_004202] -2.36 3.37E-03 A_23_P170587 SMYD2 Homo sapiens SET and MYND domain containing 2 (SMYD2), mRNA [NM_020197] -2.36 1.13E-07 A_23_P259955 GDF5 Homo sapiens growth differentiation factor 5 (GDF5), mRNA [NM_000557] -2.36 4.83E-13 A_32_P165974 GRM7 Homo sapiens glutamate receptor, metabotropic 7 (GRM7), transcript variant 2, mRNA [NM_181874] -2.36 1.80E-04 A_23_P84929 SLC38A5 Homo sapiens solute carrier family 38, member 5 (SLC38A5), mRNA [NM_033518] -2.35 1.29E-07 A_23_P342138 ADAMTSL1 Homo sapiens ADAMTS-like 1 (ADAMTSL1), transcript variant 4, mRNA [NM_001040272] -2.35 3.00E-05 A_23_P153853 ECH1 Homo sapiens enoyl CoA hydratase 1, peroxisomal (ECH1), mRNA [NM_001398] -2.35 1.31E-07 A_33_P3252925 PHF17 Homo sapiens PHD finger protein 17 (PHF17), transcript variant L, mRNA [NM_199320] -2.34 1.35E-07 A_23_P300124 SMCR8 Homo sapiens Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8), mRNA -2.34 2.27E-07 [NM_144775] A_33_P3297345 TTC24 Homo sapiens tetratricopeptide repeat domain 24 (TTC24), mRNA [NM_001105669] -2.34 1.43E-07 A_23_P139471 RPS26 Homo sapiens ribosomal protein S26 (RPS26), mRNA [NM_001029] -2.33 1.21E-12 A_23_P422193 SUV39H1 Homo sapiens suppressor of variegation 3-9 homolog 1 (Drosophila) (SUV39H1), mRNA [NM_003173] -2.33 1.38E-13 A_24_P244356 NLRX1 Homo sapiens NLR family member X1 (NLRX1), transcript variant 2, mRNA [NM_170722] -2.32 1.65E-07 A_23_P86751 ADARB2 Homo sapiens adenosine deaminase, RNA-specific, B2 (ADARB2), mRNA [NM_018702] -2.32 6.25E-07 A_33_P3389573 CARD9 Homo sapiens caspase recruitment domain family, member 9 (CARD9), transcript variant 2, mRNA -2.32 8.00E-05 [NM_052814] A_23_P200976 HYI Homo sapiens hydroxypyruvate isomerase (putative) (HYI), transcript variant 3, mRNA -2.32 1.78E-07 [NM_001190880] A_23_P256956 KIF20A Homo sapiens kinesin family member 20A (KIF20A), mRNA [NM_005733] -2.32 0.00E+00 A_33_P3211956 RGS19 Homo sapiens regulator of G-protein signaling 19 (RGS19), transcript variant 1, mRNA [NM_005873] -2.32 1.59E-13 A_24_P336848 ACYP2 Homo sapiens acylphosphatase 2, muscle type (ACYP2), mRNA [NM_138448] -2.30 2.51E-13 A_33_P3290909 SMC1A Homo sapiens structural maintenance of chromosomes 1A (SMC1A), mRNA [NM_006306] -2.30 2.10E-07 A_33_P3237359 HMGB3 Homo sapiens high-mobility group box 3 (HMGB3), mRNA [NM_005342] -2.30 2.13E-07 A_23_P73763 LAGE3 Homo sapiens L antigen family, member 3 (LAGE3), mRNA [NM_006014] -2.30 2.13E-07 A_23_P388855 MYST4 Homo sapiens MYST histone acetyltransferase (monocytic leukemia) 4 (MYST4), mRNA [NM_012330] -2.30 2.32E-07 A_24_P920125 PPIA Homo sapiens peptidylprolyl isomerase A (cyclophilin A) (PPIA), mRNA [NM_021130] -2.30 2.69E-13 A_33_P3298861 SLC12A8 Homo sapiens solute carrier family 12 (potassium/chloride transporters), member 8 (SLC12A8), -2.29 2.89E-03 transcript variant 1, mRNA [NM_024628] A_33_P3387050 C8orf82 Homo sapiens chromosome 8 open reading frame 82 (C8orf82), mRNA [NM_001001795] -2.29 2.43E-07 A_33_P3316953 RBFOX3 Homo sapiens RNA binding protein, fox-1 homolog (C. elegans) 3 (RBFOX3), mRNA [NM_001082575] -2.29 4.02E-07 A_23_P50839 MBD3 Homo sapiens methyl-CpG binding domain protein 3 (MBD3), mRNA [NM_003926] -2.29 3.14E-13 A_23_P165414 KLHL23 Homo sapiens kelch-like 23 (Drosophila) (KLHL23), mRNA [NM_144711] -2.28 2.75E-07 A_24_P319684 CCDC121 Homo sapiens coiled-coil domain containing 121 (CCDC121), transcript variant 3, mRNA [NM_024584] -2.28 1.60E-04 A_24_P29117 SLC26A1 Homo sapiens solute carrier family 26 (sulfate transporter), member 1 (SLC26A1), transcript variant 2, -2.28 6.90E-07 mRNA [NM_134425] A_23_P57868 ACY1 Homo sapiens aminoacylase 1 (ACY1), transcript variant 1, mRNA [NM_000666] -2.28 3.71E-13 A_24_P925664 MDM2 Homo sapiens Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mRNA -2.28 3.88E-13 [NM_002392] A_23_P250245 CD72 Homo sapiens CD72 molecule (CD72), mRNA [NM_001782] -2.28 4.30E-13 A_23_P145657 STAG3 Homo sapiens stromal antigen 3 (STAG3), mRNA [NM_012447] -2.28 2.91E-07 A_23_P365614 NOTCH4 Homo sapiens notch 4 (NOTCH4), mRNA [NM_004557] -2.28 1.17E-11 A_33_P3388636 FLJ40606 Homo sapiens cDNA FLJ40606 fis, clone THYMU2011939. [AK097925] -2.28 4.00E-05 A_23_P65741 DIS3L Homo sapiens DIS3 mitotic control homolog (S. cerevisiae)-like (DIS3L), transcript variant 2, mRNA -2.27 2.93E-07 [NM_133375] A_23_P152234 CMTM2 Homo sapiens CKLF-like MARVEL transmembrane domain containing 2 (CMTM2), transcript variant 1, -2.27 2.94E-07 mRNA [NM_144673] A_23_P142878 ATOH8 Homo sapiens atonal homolog 8 (Drosophila) (ATOH8), mRNA [NM_032827] -2.27 6.37E-03 A_33_P3377649 PRSS16 Homo sapiens protease, serine, 16 (thymus) (PRSS16), mRNA [NM_005865] -2.27 1.61E-03 A_23_P131646 RPIA Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA [NM_144563] -2.27 3.14E-07 A_23_P5221 ZNF333 Homo sapiens zinc finger protein 333 (ZNF333), mRNA [NM_032433] -2.27 4.38E-07 A_23_P202004 PRTFDC1 Homo sapiens phosphoribosyl transferase domain containing 1 (PRTFDC1), mRNA [NM_020200] -2.27 3.48E-07 A_33_P3276455 KDM5B Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA [NM_006618] -2.27 3.18E-07 A_33_P3272808 PTCD3 Homo sapiens Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial -2.27 4.19E-03 protein, mRNA [NM_017952] A_33_P3279470 AGRP Homo sapiens agouti related protein homolog (mouse) (AGRP), mRNA [NM_001138] -2.27 6.81E-11 A_23_P21207 UBA7 Homo sapiens ubiquitin-like modifier activating enzyme 7 (UBA7), mRNA [NM_003335] -2.26 7.47E-13 A_33_P3258223 MCM7 Homo sapiens minichromosome maintenance complex component 7 (MCM7), transcript variant 1, -2.26 3.61E-07 mRNA [NM_005916] A_23_P119344 TEAD2 Homo sapiens TEA domain family member 2 (TEAD2), mRNA [NM_003598] -2.25 1.40E-04

20 Bastian et al. 2012

A_23_P167559 RNF145 Homo sapiens ring finger protein 145 (RNF145), transcript variant 2, mRNA [NM_144726] -2.25 3.96E-07 A_23_P351535 DBIL5P Homo sapiens diazepam binding inhibitor-like 5, pseudogene (DBIL5P), non-coding RNA [NR_024120] -2.25 1.40E-06 A_33_P3301469 ANKRD30BL Homo sapiens ankyrin repeat domain 30B-like (ANKRD30BL), transcript variant 1, non-coding RNA -2.24 4.24E-07 [NR_027019] A_33_P3315410 COIL Homo sapiens coilin (COIL), mRNA [NM_004645] -2.24 9.15E-13 A_24_P160874 DUT Homo sapiens deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, -2.24 4.40E-07 transcript variant 1, mRNA [NM_001025248] A_24_P218979 CDCA3 Homo sapiens cell division cycle associated 3 (CDCA3), mRNA [NM_031299] -2.24 4.41E-07 A_23_P62227 CXorf21 Homo sapiens chromosome X open reading frame 21 (CXorf21), mRNA [NM_025159] -2.24 4.56E-07 A_33_P3240353 SLC39A4 Homo sapiens solute carrier family 39 (zinc transporter), member 4 (SLC39A4), transcript variant 1, -2.23 1.07E-12 mRNA [NM_017767] A_23_P130961 ELANE Homo sapiens elastase, neutrophil expressed (ELANE), mRNA [NM_001972] -2.23 1.30E-03 A_23_P138480 C10orf95 Homo sapiens chromosome 10 open reading frame 95 (C10orf95), mRNA [NM_024886] -2.23 1.78E-06 A_33_P3302957 PLEKHG4 Homo sapiens pleckstrin homology domain containing, family G (with RhoGef domain) member 4 -2.23 5.95E-03 (PLEKHG4), transcript variant 1, mRNA [NM_015432] A_33_P3357979 CTBP1 Homo sapiens C-terminal binding protein 1 (CTBP1), transcript variant 2, mRNA [NM_001012614] -2.21 6.14E-07 A_33_P3330841 HUS1 Homo sapiens HUS1 checkpoint homolog (S. pombe) (HUS1), mRNA [NM_004507] -2.21 5.77E-08 A_33_P3781228 FLJ26332 Homo sapiens cDNA FLJ26332 fis, clone HRT02453. [AK129842] -2.21 3.00E-05 A_23_P65129 SPRYD3 Homo sapiens SPRY domain containing 3 (SPRYD3), mRNA [NM_032840] -2.21 7.23E-07 A_23_P132856 LRRC33 Homo sapiens leucine rich repeat containing 33 (LRRC33), mRNA [NM_198565] -2.20 6.85E-07 A_23_P29594 RPL39L Homo sapiens ribosomal protein L39-like (RPL39L), mRNA [NM_052969] -2.20 6.74E-07 A_23_P361381 DMC1 Homo sapiens DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous -2.20 1.21E-11 recombination (yeast) (DMC1), mRNA [NM_007068] A_24_P103469 LST1 Homo sapiens leukocyte specific transcript 1 (LST1), transcript variant 1, mRNA [NM_007161] -2.20 2.41E-12 A_24_P338187 HNRNPH3 Homo sapiens heterogeneous nuclear ribonucleoprotein H3 (2H9) (HNRNPH3), transcript variant 2H9, -2.20 2.35E-12 mRNA [NM_012207] A_23_P113393 APLN Homo sapiens apelin (APLN), mRNA [NM_017413] -2.20 1.26E-14 A_33_P3265803 DEFB108B Homo sapiens defensin, beta 108B (DEFB108B), mRNA [NM_001002035] -2.19 7.33E-07 A_33_P3209491 TNS1 Homo sapiens tensin 1 (TNS1), mRNA [NM_022648] -2.19 7.33E-07 A_24_P101391 YBX1 Homo sapiens Y box binding protein 1 (YBX1), mRNA [NM_004559] -2.19 7.36E-07 A_23_P303671 ECM2 Homo sapiens extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript -2.19 9.30E-04 variant 1, mRNA [NM_001393] A_24_P196117 GTF2H5 Homo sapiens general transcription factor IIH, polypeptide 5 (GTF2H5), mRNA [NM_207118] -2.19 7.76E-07 A_33_P3248108 ZNF618 Homo sapiens zinc finger protein 618 (ZNF618), mRNA [NM_133374] -2.19 9.92E-07 A_32_P126733 NOL4 Homo sapiens nucleolar protein 4 (NOL4), transcript variant 1, mRNA [NM_003787] -2.18 9.03E-07 A_33_P3349827 RBM3 Homo sapiens RNA binding motif (RNP1, RRM) protein 3 (RBM3), mRNA [NM_006743] -2.17 9.60E-07 A_23_P167585 GDF9 Homo sapiens growth differentiation factor 9 (GDF9), mRNA [NM_005260] -2.17 8.52E-06 A_23_P419038 CLOCK Homo sapiens clock homolog (mouse) (CLOCK), mRNA [NM_004898] -2.17 1.92E-10 A_33_P3383422 DHRS4 Homo sapiens dehydrogenase/reductase (SDR family) member 4 (DHRS4), mRNA [NM_021004] -2.17 9.83E-07 A_23_P46309 RCC1 Homo sapiens regulator of chromosome condensation 1 (RCC1), transcript variant 4, mRNA -2.17 4.58E-12 [NM_001048199] A_23_P352266 BCL2 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript -2.17 0.00E+00 variant alpha, mRNA [NM_000633] A_24_P297539 UBE2C Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 6, mRNA [NM_181803] -2.17 4.74E-12

A_24_P261005 C8orf40 Homo sapiens chromosome 8 open reading frame 40 (C8orf40), transcript variant 3, mRNA -2.17 1.02E-06 [NM_138436] A_24_P275828 PGAP3 Homo sapiens post-GPI attachment to proteins 3 (PGAP3), mRNA [NM_033419] -2.17 1.05E-06 A_23_P143016 ARID5A Homo sapiens AT rich interactive domain 5A (MRF1-like) (ARID5A), mRNA [NM_212481] -2.17 1.03E-06 A_33_P3507270 EFCAB5 Homo sapiens EF-hand calcium binding domain 5 (EFCAB5), transcript variant 1, mRNA [NM_198529] -2.16 6.56E-06 A_33_P3222630 FBXO43 Homo sapiens F-box protein 43 (FBXO43), transcript variant 2, mRNA [NM_001029860] -2.16 1.11E-06 A_24_P376707 HDGF Homo sapiens hepatoma-derived growth factor (HDGF), transcript variant 1, mRNA [NM_004494] -2.16 5.64E-12 A_33_P3347976 MIB1 Homo sapiens mindbomb homolog 1 (Drosophila) (MIB1), mRNA [NM_020774] -2.16 5.96E-12 A_23_P428640 PPP1R3E Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 3E (PPP1R3E), non-coding RNA -2.16 1.36E-06 [NR_026862] A_23_P101960 ZFP36L2 Homo sapiens zinc finger protein 36, C3H type-like 2 (ZFP36L2), mRNA [NM_006887] -2.16 0.00E+00 A_33_P3296169 CDCA7 Homo sapiens cell division cycle associated 7 (CDCA7), transcript variant 1, mRNA [NM_031942] -2.16 1.15E-06 A_32_P86739 C10orf114 Homo sapiens chromosome 10 open reading frame 114 (C10orf114), mRNA [NM_001010911] -2.16 1.30E-06 A_23_P170978 ZNF692 Homo sapiens zinc finger protein 692 (ZNF692), transcript variant 2, mRNA [NM_017865] -2.16 1.15E-06 A_23_P120931 APOBEC3C Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C (APOBEC3C), -2.16 0.00E+00 mRNA [NM_014508] A_24_P65199 CDK10 Homo sapiens cyclin-dependent kinase 10 (CDK10), transcript variant b, mRNA [NM_052987] -2.15 1.37E-06 A_23_P1043 C1orf106 Homo sapiens chromosome 1 open reading frame 106 (C1orf106), transcript variant 1, mRNA -2.15 1.86E-03 [NM_018265] A_24_P923102 PHOSPHO2- Homo sapiens PHOSPHO2-KLHL23 read-through transcript (PHOSPHO2-KLHL23), mRNA -2.15 1.34E-06 KLHL23 [NM_001199290] A_24_P342312 ODZ4 Homo sapiens odz, odd Oz/ten-m homolog 4 (Drosophila) (ODZ4), mRNA [NM_001098816] -2.15 1.86E-06 A_23_P96599 TMSB15B Homo sapiens thymosin beta 15B (TMSB15B), mRNA [NM_194324] -2.15 1.21E-06 A_33_P3259022 SLC6A2 Homo sapiens solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 (SLC6A2), -2.15 1.32E-06 transcript variant 1, mRNA [NM_001172504] A_24_P126557 RAVER1 Homo sapiens ribonucleoprotein, PTB-binding 1 (RAVER1), mRNA [NM_133452] -2.15 1.63E-06 A_33_P3404651 TTC7A Homo sapiens tetratricopeptide repeat domain 7A (TTC7A), mRNA [NM_020458] -2.15 1.26E-06 A_23_P124733 COQ2 Homo sapiens coenzyme Q2 homolog, prenyltransferase (yeast) (COQ2), nuclear gene encoding -2.15 1.06E-11 mitochondrial protein, mRNA [NM_015697] A_23_P87603 TARBP2 Homo sapiens TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 1, mRNA [NM_134323] -2.14 8.03E-12 A_23_P137504 ZBTB37 Homo sapiens zinc finger and BTB domain containing 37 (ZBTB37), transcript variant 2, mRNA -2.14 3.00E-05 [NM_032522] A_23_P302134 FLJ37453 Homo sapiens hypothetical LOC729614 (FLJ37453), non-coding RNA [NR_024279] -2.14 1.36E-06 A_33_P3280094 LRFN4 Homo sapiens leucine rich repeat and fibronectin type III domain containing 4 (LRFN4), mRNA -2.14 1.38E-06 [NM_024036]

21 Bastian et al. 2012

A_33_P3329607 RAB1B Homo sapiens RAB1B, member RAS oncogene family (RAB1B), mRNA [NM_030981] -2.14 1.51E-06 A_24_P268676 BHLHE40 Homo sapiens basic helix-loop-helix family, member e40 (BHLHE40), mRNA [NM_003670] -2.14 1.59E-11 A_23_P377214 HEXIM2 Homo sapiens hexamthylene bis-acetamide inducible 2 (HEXIM2), mRNA [NM_144608] -2.14 1.45E-06 A_33_P3381899 SYNGR3 Homo sapiens synaptogyrin 3 (SYNGR3), mRNA [NM_004209] -2.14 2.78E-06 A_33_P3341716 SP4 Homo sapiens Sp4 transcription factor (SP4), mRNA [NM_003112] -2.13 9.97E-12 A_33_P3255794 POLE Homo sapiens polymerase (DNA directed), epsilon (POLE), mRNA [NM_006231] -2.13 2.76E-06 A_23_P131299 ZFAND2B Homo sapiens zinc finger, AN1-type domain 2B (ZFAND2B), mRNA [NM_138802] -2.13 1.53E-06 A_23_P162525 UTP20 Homo sapiens UTP20, small subunit (SSU) processome component, homolog (yeast) (UTP20), mRNA -2.13 1.54E-06 [NM_014503] A_23_P35871 E2F8 Homo sapiens E2F transcription factor 8 (E2F8), mRNA [NM_024680] -2.13 1.55E-06 A_23_P143535 WDR4 Homo sapiens WD repeat domain 4 (WDR4), transcript variant 2, mRNA [NM_033661] -2.13 2.33E-06 A_33_P3262431 KREMEN1 Homo sapiens kringle containing transmembrane protein 1 (KREMEN1), transcript variant 2, mRNA -2.13 5.00E-04 [NM_032045] A_23_P9603 PRKDC Homo sapiens protein kinase, DNA-activated, catalytic polypeptide (PRKDC), transcript variant 1, -2.13 1.16E-11 mRNA [NM_006904] A_33_P3302796 U2AF2 Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 1, mRNA -2.13 1.15E-11 [NM_007279] A_23_P149200 CDC20 Homo sapiens cell division cycle 20 homolog (S. cerevisiae) (CDC20), mRNA [NM_001255] -2.13 1.16E-11 A_33_P3271105 PABPC4 Homo sapiens poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 3, -2.13 5.00E-05 mRNA [NM_001135654] A_24_P212127 ZNF33A Homo sapiens zinc finger protein 33A (ZNF33A), transcript variant 1, mRNA [NM_006954] -2.12 1.74E-06 A_24_P179816 SLC27A3 Homo sapiens solute carrier family 27 (fatty acid transporter), member 3 (SLC27A3), mRNA -2.12 1.69E-06 [NM_024330] A_23_P85800 CD52 Homo sapiens CD52 molecule (CD52), mRNA [NM_001803] -2.12 1.29E-11 A_33_P3336252 OR7E37P Homo sapiens olfactory receptor, family 7, subfamily E, member 37 pseudogene (OR7E37P), non- -2.12 1.67E-03 coding RNA [NR_002163] A_33_P3296871 ZNF33B Homo sapiens zinc finger protein 33B (ZNF33B), mRNA [NM_006955] -2.12 1.78E-06 A_23_P79794 TGIF2 Homo sapiens TGFB-induced factor homeobox 2 (TGIF2), transcript variant 2, mRNA [NM_021809] -2.12 2.55E-06 A_33_P3380161 NCRNA00201 Homo sapiens non-protein coding RNA 201 (NCRNA00201), non-coding RNA [NR_026778] -2.12 1.80E-06 A_33_P3273148 GADD45GIP1 Homo sapiens growth arrest and DNA-damage-inducible, gamma interacting protein 1 (GADD45GIP1), -2.12 1.79E-06 mRNA [NM_052850] A_24_P145122 NUCKS1 Homo sapiens nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1), mRNA -2.12 1.66E-11 [NM_022731] A_33_P3247022 CCNE2 Homo sapiens cyclin E2 (CCNE2), mRNA [NM_057749] -2.12 1.60E-11 A_23_P155969 PLK4 Homo sapiens polo-like kinase 4 (PLK4), transcript variant 1, mRNA [NM_014264] -2.12 1.71E-11 A_23_P359904 PDDC1 Homo sapiens Parkinson disease 7 domain containing 1 (PDDC1), mRNA [NM_182612] -2.11 1.89E-06 A_33_P3412613 TMPO Homo sapiens thymopoietin (TMPO), transcript variant 2, mRNA [NM_001032283] -2.11 1.66E-11 A_23_P47517 VPS37C Homo sapiens vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C), mRNA [NM_017966] -2.11 2.04E-06 A_24_P33444 YWHAE Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon -2.11 2.07E-06 polypeptide (YWHAE), transcript variant 1, mRNA [NM_006761] A_33_P3276703 VGF Homo sapiens VGF nerve growth factor inducible (VGF), mRNA [NM_003378] -2.11 2.03E-11 A_33_P3297255 MXD3 Homo sapiens MAX dimerization protein 3 (MXD3), transcript variant 1, mRNA [NM_031300] -2.11 1.91E-11 A_24_P365365 TCF3 Homo sapiens transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), -2.11 1.94E-11 transcript variant 1, mRNA [NM_003200] A_23_P421011 KAZALD1 Homo sapiens Kazal-type serine peptidase inhibitor domain 1 (KAZALD1), mRNA [NM_030929] -2.11 2.09E-06 A_33_P3238455 SRSF6 Homo sapiens serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 2, non-coding RNA -2.11 2.45E-06 [NR_034009] A_33_P3424207 RABEP2 Homo sapiens rabaptin, RAB GTPase binding effector protein 2 (RABEP2), mRNA [NM_024816] -2.11 2.11E-06 A_33_P3409124 NR1D2 Homo sapiens nuclear receptor subfamily 1, group D, member 2 (NR1D2), transcript variant 1, mRNA -2.10 8.66E-08 [NM_005126] A_23_P403955 TARDBP Homo sapiens TAR DNA binding protein (TARDBP), mRNA [NM_007375] -2.10 2.22E-06 A_33_P3249936 C3orf67 Homo sapiens chromosome 3 open reading frame 67 (C3orf67), mRNA [NM_198463] -2.10 2.24E-06 A_24_P168416 PRDX2 Homo sapiens peroxiredoxin 2 (PRDX2), nuclear gene encoding mitochondrial protein, transcript -2.10 2.22E-11 variant 3, mRNA [NM_181738] A_24_P130936 DDX3Y Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2, -2.10 2.00E-05 mRNA [NM_004660] A_33_P3382423 ZNF428 Homo sapiens zinc finger protein 428 (ZNF428), mRNA [NM_182498] -2.10 2.35E-06 A_24_P120115 CFLAR Homo sapiens CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1, mRNA -2.10 3.79E-10 [NM_003879] A_23_P118174 PLK1 Homo sapiens polo-like kinase 1 (PLK1), mRNA [NM_005030] -2.10 2.50E-11 A_33_P3232955 F2RL3 Homo sapiens coagulation factor II (thrombin) receptor-like 3 (F2RL3), mRNA [NM_003950] -2.10 3.84E-11 A_33_P3887888 FLJ44511 Homo sapiens hypothetical LOC441307 (FLJ44511), non-coding RNA [NR_033963] -2.09 6.62E-03 A_23_P349928 SP100 Homo sapiens SP100 nuclear antigen (SP100), transcript variant 2, mRNA [NM_003113] -2.09 2.67E-11 A_23_P258493 LMNB1 Homo sapiens lamin B1 (LMNB1), transcript variant 1, mRNA [NM_005573] -2.09 2.72E-11 A_23_P138125 FAIM3 Homo sapiens Fas apoptotic inhibitory molecule 3 (FAIM3), transcript variant 1, mRNA [NM_005449] -2.09 6.33E-11 A_23_P335239 GAB1 Homo sapiens GRB2-associated binding protein 1 (GAB1), transcript variant 1, mRNA [NM_207123] -2.09 3.19E-11 A_23_P28652 C2orf28 Homo sapiens chromosome 2 open reading frame 28 (C2orf28), transcript variant 1, mRNA -2.09 2.59E-06 [NM_016085] A_24_P283928 OBFC2B Homo sapiens oligonucleotide/oligosaccharide-binding fold containing 2B (OBFC2B), mRNA -2.09 2.62E-06 [NM_024068] A_23_P357101 APOBEC3F Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F (APOBEC3F), -2.09 3.40E-11 transcript variant 1, mRNA [NM_145298] A_33_P3398840 ZBED1 Homo sapiens zinc finger, BED-type containing 1 (ZBED1), transcript variant 3, mRNA -2.09 3.72E-06 [NM_001171135] A_23_P306941 RGL4 Homo sapiens ral guanine nucleotide dissociation stimulator-like 4 (RGL4), mRNA [NM_153615] -2.09 2.89E-06 A_23_P150394 FXYD6 Homo sapiens FXYD domain containing ion transport regulator 6 (FXYD6), transcript variant 1, mRNA -2.09 1.10E-04 [NM_022003] A_23_P31135 ACAT2 Homo sapiens acetyl-CoA acetyltransferase 2 (ACAT2), mRNA [NM_005891] -2.08 3.29E-11 A_33_P3306545 EIF4G2 Homo sapiens eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 3, mRNA -2.08 2.74E-06 [NM_001172705]

22 Bastian et al. 2012

A_23_P35791 RPS6KA4 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1, -2.08 3.39E-11 mRNA [NM_003942] A_32_P475513 MYO15B Homo sapiens myosin XVB pseudogene (MYO15B), non-coding RNA [NR_003587] -2.08 3.79E-06 A_33_P3630129 FLJ46875 Homo sapiens cDNA FLJ46875 fis, clone UTERU3014446. [AK128708] -2.08 1.63E-03 A_23_P128351 EFCAB4B Homo sapiens EF-hand calcium binding domain 4B (EFCAB4B), transcript variant 3, mRNA -2.08 6.23E-06 [NM_032680] A_23_P362659 MYD88 Homo sapiens myeloid differentiation primary response gene (88) (MYD88), transcript variant 2, mRNA -2.08 3.52E-11 [NM_002468] A_24_P182947 GCET2 Homo sapiens germinal center expressed transcript 2 (GCET2), transcript variant 3, mRNA -2.08 3.62E-11 [NM_001190259] A_24_P290770 OGDH Homo sapiens oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (OGDH), nuclear gene -2.08 2.06E-08 encoding mitochondrial protein, transcript variant 2, mRNA [NM_001003941] A_23_P301414 ERG Homo sapiens v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 2, -2.08 3.99E-11 mRNA [NM_004449] A_33_P3414202 MBNL3 Homo sapiens muscleblind-like 3 (Drosophila) (MBNL3), transcript variant 6, mRNA [NM_001170704] -2.08 3.03E-06 A_32_P63848 OXCT1 Homo sapiens 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, -2.08 4.05E-11 mRNA [NM_000436] A_32_P109572 HNRNPL Homo sapiens heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 1, mRNA -2.08 4.09E-11 [NM_001533] A_33_P3385782 ZNF713 Homo sapiens zinc finger protein 713 (ZNF713), mRNA [NM_182633] -2.08 3.00E-05 A_33_P3330498 ALDH7A1 aldehyde dehydrogenase 7 family, member A1 [Source:HGNC Symbol;Acc:877] [ENST00000409134] -2.08 2.77E-10 A_33_P3319572 FAM71E2 Homo sapiens family with sequence similarity 71, member E2 (FAM71E2), mRNA [NM_001145402] -2.07 3.27E-06 A_23_P378416 GPM6B Homo sapiens glycoprotein M6B (GPM6B), transcript variant 2, mRNA [NM_001001996] -2.07 1.43E-08 A_23_P125618 GABRA3 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3), mRNA [NM_000808] -2.07 4.26E-11 A_23_P150903 FAR2 Homo sapiens fatty acyl CoA reductase 2 (FAR2), mRNA [NM_018099] -2.07 3.96E-06 A_23_P116557 LGALS9 Homo sapiens lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 1, mRNA -2.07 3.21E-06 [NM_009587] A_23_P132277 MCM5 Homo sapiens minichromosome maintenance complex component 5 (MCM5), mRNA [NM_006739] -2.07 5.31E-11 A_24_P832426 B3GALTL Homo sapiens beta 1,3-galactosyltransferase-like (B3GALTL), mRNA [NM_194318] -2.06 6.02E-11 A_23_P393749 CATSPER3 Homo sapiens cation channel, sperm associated 3 (CATSPER3), mRNA [NM_178019] -2.06 7.92E-06 A_23_P155765 HMGB2 Homo sapiens high-mobility group box 2 (HMGB2), transcript variant 1, mRNA [NM_002129] -2.06 5.59E-11 A_32_P218228 FAM109B Homo sapiens family with sequence similarity 109, member B (FAM109B), mRNA [NM_001002034] -2.06 3.00E-05 A_23_P77437 PRMT7 Homo sapiens protein arginine methyltransferase 7 (PRMT7), transcript variant 1, mRNA [NM_019023] -2.06 4.05E-06 A_33_P3232006 UBL4A Homo sapiens ubiquitin-like 4A (UBL4A), mRNA [NM_014235] -2.06 3.68E-06 A_24_P93371 COMMD4 Homo sapiens COMM domain containing 4 (COMMD4), mRNA [NM_017828] -2.06 3.74E-06 A_24_P89080 DCK Homo sapiens deoxycytidine kinase (DCK), mRNA [NM_000788] -2.06 6.68E-11 A_33_P3243168 MZF1 Homo sapiens myeloid zinc finger 1 (MZF1), transcript variant 2, mRNA [NM_198055] -2.05 6.70E-11 A_24_P941866 C5orf56 Homo sapiens chromosome 5 open reading frame 56 (C5orf56), mRNA [NM_001013717] -2.05 1.00E-05 A_23_P130182 AURKB Homo sapiens aurora kinase B (AURKB), mRNA [NM_004217] -2.05 6.94E-11 A_33_P3244122 HAAO Homo sapiens 3-hydroxyanthranilate 3,4-dioxygenase (HAAO), mRNA [NM_012205] -2.05 4.36E-06 A_33_P3331601 THNSL1 Homo sapiens threonine synthase-like 1 (S. cerevisiae) (THNSL1), mRNA [NM_024838] -2.05 6.46E-06 A_24_P450285 CCDC153 Homo sapiens coiled-coil domain containing 153 (CCDC153), mRNA [NM_001145018] -2.05 1.00E-05 A_33_P3317628 PKP3 Homo sapiens plakophilin 3 (PKP3), mRNA [NM_007183] -2.05 8.54E-11 A_33_P3271657 HHIPL1 Homo sapiens HHIP-like 1 (HHIPL1), transcript variant 1, mRNA [NM_001127258] -2.05 4.72E-06 A_23_P48237 MCRS1 Homo sapiens microspherule protein 1 (MCRS1), transcript variant 1, mRNA [NM_006337] -2.04 8.89E-11 A_23_P44505 KLF11 Homo sapiens Kruppel-like factor 11 (KLF11), transcript variant 1, mRNA [NM_003597] -2.04 9.85E-11 A_33_P3215422 LYL1 Homo sapiens lymphoblastic leukemia derived sequence 1 (LYL1), mRNA [NM_005583] -2.04 9.49E-11 A_23_P52986 VWCE Homo sapiens von Willebrand factor C and EGF domains (VWCE), mRNA [NM_152718] -2.04 4.66E-06 A_33_P3422404 ACSS1 Homo sapiens acyl-CoA synthetase short-chain family member 1 (ACSS1), nuclear gene encoding -2.04 7.31E-06 mitochondrial protein, mRNA [NM_032501] A_23_P164814 C19orf57 Homo sapiens chromosome 19 open reading frame 57 (C19orf57), mRNA [NM_024323] -2.04 4.76E-06 A_23_P92520 ANP32C Homo sapiens acidic (leucine-rich) nuclear phosphoprotein 32 family, member C (ANP32C), mRNA -2.04 4.91E-06 [NM_012403] A_24_P330633 TAT Homo sapiens tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein, mRNA -2.04 3.00E-05 [NM_000353] A_23_P73780 IRAK1 Homo sapiens interleukin-1 receptor-associated kinase 1 (IRAK1), transcript variant 1, mRNA -2.03 1.18E-10 [NM_001569] A_33_P3322589 FANCL Homo sapiens Fanconi anemia, complementation group L (FANCL), transcript variant 1, mRNA -2.03 5.32E-06 [NM_001114636] A_24_P148043 FAM20B Homo sapiens family with sequence similarity 20, member B (FAM20B), mRNA [NM_014864] -2.03 5.25E-06 A_32_P377032 C2orf52 Homo sapiens chromosome 2 open reading frame 52 (C2orf52), non-coding RNA [NR_024079] -2.03 2.00E-04 A_33_P3391290 C5orf13 Homo sapiens chromosome 5 open reading frame 13 (C5orf13), transcript variant 11, mRNA -2.03 5.45E-06 [NM_001142483] A_24_P305764 SMS Homo sapiens spermine synthase (SMS), mRNA [NM_004595] -2.02 5.67E-06 A_23_P43580 CEP110 Homo sapiens centrosomal protein 110kDa (CEP110), mRNA [NM_007018] -2.02 1.65E-10 A_23_P212400 NAT6 Homo sapiens N-acetyltransferase 6 (GCN5-related) (NAT6), transcript variant 1, mRNA [NM_012191] -2.02 8.25E-06 A_24_P138022 FAM120A Homo sapiens family with sequence similarity 120A (FAM120A), mRNA [NM_014612] -2.02 5.94E-06 A_33_P3409934 SEH1L Homo sapiens SEH1-like (S. cerevisiae) (SEH1L), transcript variant 1, mRNA [NM_001013437] -2.02 6.26E-06 A_33_P3243264 DNLZ Homo sapiens DNL-type zinc finger (DNLZ), mRNA [NM_001080849] -2.02 6.17E-06 A_32_P228699 PRDM10 Homo sapiens PR domain containing 10 (PRDM10), transcript variant 2, mRNA [NM_199437] -2.02 6.47E-06 A_23_P210015 PTPN18 Homo sapiens protein tyrosine phosphatase, non-receptor type 18 (brain-derived) (PTPN18), transcript -2.02 6.32E-06 variant 1, mRNA [NM_014369] A_23_P55286 OMG Homo sapiens oligodendrocyte myelin glycoprotein (OMG), mRNA [NM_002544] -2.02 3.70E-04 A_33_P3360675 PRKAR1B Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant -2.02 8.13E-06 1, mRNA [NM_001164761] A_23_P87580 ANP32D Homo sapiens acidic (leucine-rich) nuclear phosphoprotein 32 family, member D (ANP32D), mRNA -2.01 6.53E-06 [NM_012404] A_33_P3489646 SPI1 Homo sapiens spleen focus forming virus (SFFV) proviral integration oncogene spi1 (SPI1), transcript -2.01 1.90E-10 variant 1, mRNA [NM_001080547]

23 Bastian et al. 2012

A_23_P40049 CAD Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase -2.01 1.89E-10 (CAD), mRNA [NM_004341] A_33_P3636590 SUCLG2 Homo sapiens succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding -2.01 8.16E-06 mitochondrial protein, transcript variant 2, mRNA [NM_003848] A_24_P65941 C21orf96 Homo sapiens chromosome 21 open reading frame 96 (C21orf96), non-coding RNA [NR_026812] -2.01 7.40E-06 A_23_P143190 MYBL2 Homo sapiens v-myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2), mRNA -2.01 1.96E-10 [NM_002466] A_24_P941912 DTX3L Homo sapiens deltex 3-like (Drosophila) (DTX3L), mRNA [NM_138287] -2.01 6.97E-06 A_23_P88880 ZNF423 Homo sapiens zinc finger protein 423 (ZNF423), mRNA [NM_015069] -2.01 6.94E-06 A_23_P250629 PSMB8 Homo sapiens proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase -2.01 2.01E-10 7) (PSMB8), transcript variant 1, mRNA [NM_004159] A_32_P210202 E2F7 Homo sapiens E2F transcription factor 7 (E2F7), mRNA [NM_203394] -2.01 2.04E-10 A_24_P96780 CENPF Homo sapiens centromere protein F, 350/400kDa (mitosin) (CENPF), mRNA [NM_016343] -2.01 7.02E-06 A_33_P3394494 C17orf81 Homo sapiens chromosome 17 open reading frame 81 (C17orf81), transcript variant 2, mRNA -2.01 6.99E-06 [NM_203413] A_33_P3329344 FASN Homo sapiens fatty acid synthase (FASN), mRNA [NM_004104] -2.01 2.10E-10 A_24_P346855 MKI67 Homo sapiens antigen identified by monoclonal antibody Ki-67 (MKI67), transcript variant 1, mRNA -2.01 2.16E-10 [NM_002417] A_24_P330822 HNF1B Homo sapiens HNF1 homeobox B (HNF1B), transcript variant 1, mRNA [NM_000458] -2.01 3.17E-08 A_24_P904903 BAT1 Homo sapiens HLA-B associated transcript 1 (BAT1), transcript variant 1, mRNA [NM_004640] -2.01 2.32E-10 A_23_P102937 SUMO3 Homo sapiens SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) (SUMO3), mRNA [NM_006936] -2.00 1.12E-44 A_32_P186027 ANP32A Homo sapiens acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), mRNA -2.00 2.27E-10 [NM_006305] A_23_P122228 NDUFS6 Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q -2.00 1.40E-45 reductase) (NDUFS6), nuclear gene encoding mitochondrial protein, mRNA [NM_004553] A_33_P3344603 ZNF280D Homo sapiens zinc finger protein 280D (ZNF280D), transcript variant 3, mRNA [NM_001002844] -2.00 4.10E-04 A_32_P163247 CD8A Homo sapiens CD8a molecule (CD8A), transcript variant 1, mRNA [NM_001768] -2.00 3.26E-09 A_24_P142269 HIRIP3 Homo sapiens HIRA interacting protein 3 (HIRIP3), mRNA [NM_003609] -2.00 7.58E-06 A_23_P373119 HMGB3P1 Homo sapiens high-mobility group box 3 pseudogene 1 (HMGB3P1), non-coding RNA [NR_002165] -2.00 7.53E-06 A_33_P3412463 C10orf81 Homo sapiens chromosome 10 open reading frame 81 (C10orf81), transcript variant 2, mRNA -2.00 2.88E-03 [NM_001193434]

435 differentially downregulated genes after concomitant treatment with VPA and

BTZ are shown. Sequence code, Agilent Whole Human Genome Oligo Microarrays;

Fold Change, comparing concomitant treatment to untreated control; p - value, Two-

way ANOVA statistical analysis.

24