IDENTIFICATION of CELL SURFACE MARKERS WHICH CORRELATE WITH SALL4 in a B-CELL ACUTE LYMPHOBLASTIC LEUKEMIA WITH T(8;14) DISCOVERED THROUGH BIOINFORMATIC ANALYSIS of MICROARRAY EXPRESSION DATA

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-XQH   Thesis Advisor: Dr. Li Chai Author: Robert Paul Weinberg Department of Pathology Candidate MMSc in Immunology Brigham and Womens’ Hospital Harvard Medical School 77 Francis Street 25 Shattuck Street Boston, MA 02215 Boston, MA 02215

IDENTIFICATION OF CELL SURFACE MARKERS WHICH CORRELATE WITH SALL4 IN A B-CELL ACUTE LYMPHOBLASTIC LEUKEMIA WITH TRANSLOCATION t(8;14) DISCOVERED THROUGH BIOINFORMATICS ANALYSIS OF MICROARRAY GENE EXPRESSION DATA

Abstract

Acute Lymphoblastic Leukemia (ALL) is the most common leukemia in children, causing signficant morbidity and mortality annually in the U.S. We performed exploratory data analysis on several microarray gene expression data sets publicly available in the Gene Expression Omnibus (GEO) repository maintained at the National Center for Biotechnology Information of the National Library of

Medicine under the National Institutes of Health (http://ncbi.nlm.nih.gov) looking for novel associations and relationships between the SALL4 and leukemia.

Through this data mining, we found a subset of B-cell ALL where multiple cell surface markers have relatively high correlation with SALL4. However, in part due to the small number of samples in this group ( n = 13 ), the results of these analyses must be considered with caution until such time as they may be validated experimentally in the lab with living leukemia cells.

We evaluated the transcriptome changes in these leukemia datasets which are associated with the expression of the SALL4. The correlation analysis of the microarray data revealed that a small subset of

B-cell ALL, comprising 13 samples, a mature B-cell acute lymphoblastic leukemia with a translocation of t(8;14) subset [B-ALL with t(8;14)] has multiple cell surface marker which showed relatively high

ii

correlation with SALL4 expression ( | r | > 0.60), whereas 16 other leukemia subsets only showed low- moderate correlation of the same cell surface biomarkers with SALL4 ( | r | < 0.45).

The microarray gene expression data was obtained using the Affymetrix gene chip, HG-

U133Plus2, which is a 3’ IVT oligonucleotide array for the detection of cDNA, which is synthesized from mRNA extracted from the relevant human cells. The array consists of both Perfect Match and Mismatch probes for the detection and differential analysis of some 23,520 probe-gene pairs. The luminosity read- out from the gene chip assay then undergoes a number of statistical manipulations which include standardization and normalization of the data prior to its deposit in the GEO library. Within each dataset the gene expression data is normalized but special methods must be used if one wants to compare the data between different datasets from different experiments in the GEO repository. Some datasets include the raw luminosity read-outs.

The majority of this thesis focuses on one specific microarray gene expression dataset,

GSE13159, which comprises some 2,096 samples taken from patients with acute lymphoblastic leukemia

(ALL), (AML), chronic lymphocytic leukemia (CLL), chronic myeloid leukemia

(CML), (MDS) and normal healthy controls.

After finding the B-ALL with t(8;14) wherein the cell surface markers correlate highly with

SALL4, we used the limma package from the R-based Bioconductor platform to perform a linear regression analysis looking for the differential expression of genes in the transcriptome. The linear regression analysis reveals that this B-cell leukemia subset has genes differentially expressed distinct from the average pattern of gene expression of the other lymphoblastic leukemias.

Extensive bioinformatic analyses were carried out on this small group of samples and the limitations of these analyses will be further examined in the discussion section of the paper. Some preliminary functional genomic analysis was carried out on these differentially expressed genes (DEGs)

iii

and they were compartmentalized into specific gene ontologies (GO) and KEGG pathways, which includes the hematopoietic pathway. This corollary data can be found in the appendices attached.

There is some overlap of the Gene Ontologies and the KEGG pathways between the 17 leukemia

/ myelodysplastic groups analyzed, which includes the hematopoietic pathway but the B-ALL with t(8;14) showed differences from the other leukemias.

SALL4 is a zinc-finger transcription factor important in maintaining the pluripotency of embryonic and hematopoietic stem cells as evidenced in transgenic animal models and genetically modified cell lines with either deletion of SALL4 or forced over-expression of SALL4. Experimental evidence also suggests that SALL4 plays an important role in leukemogenesis as well as other oncogenic processes in other neoplasms.

Potentially the association found between these specific cell surface biomarkers with SALL4 expression in this B-ALL with t(8;14) subset may facilitate future research on SALL4. The iPathway tool

(www.advaitabio.com) was used to further characterize this B-ALL t(8;14) subset. The iPathway tool revealed 549 differentially expressed genes (DEGs) compared with the normal samples identified out of a total of 20,388 genes with measured expression. These 549 DEGs have a significant impact on 34 biological pathways by KEGG analysis. These 549 DEGs also comprise a significant enrichment of 1431

Gene Ontology (GO) terms, 237 predicted miRNAs and 57 diseases based on uncorrected p-values.

These DEGs were analyzed in the context of pathways from the Kyoto Encyclopedia of Genes and

Genomes (KEGG), the Consortium database (GO), the miRBase and TARGETSCAN databases. Some of the iPathway results will be found in the appendices. These results must be considered with caution considering significant limitations in this study.

iv

Table of Contents Pages

Abstract ………………………………………………………………………….. ii - iv

Table of Contents ……………………………………………………………….. v - vi

List of Figures …………………………………………………………………..… vii

List of Tables ……………………………………………………………………... viii

Acknowledgments …………………………………………………………………..ix

1. Chapter 1: Background ...... …. 1 - 5

1.1 The pluripotency-maintaining transcription Factor SALL4 ...... 1

1.3 The genetics of B-cell ALL with the t(8;14) translocation ...... 3

2. Chapter 2: Data and Methods ...... …… 5 - 31

2.1 Microarray analysis of Gene expression ………………………...... … 5

2.2 Bioinformatics and Computational Biology tools …………...... …. 7

2.3 R programming language and Bioconductor software tools ...... …. 8

2.4 Data and computational Results ...... …. 9

2.5 Brief Discussion of Results ...... …. 30

3. Chapter 3: Discussion and Perspectives ...... 33 - 36

3.1 Discussion ………………………………………………….………..………… 33

3.2 Limitations ...... …...... ………....…35

3.3 Future Research Paths ...... …...... …..…….. 36

4. Bibliography ...... …… 37 - 57

5. Appendices

Appendix A Correlation of cell surface markers for other groups ……………… 58 - 60

Appendix B Biology and Genetics of Acute Lymphoblastic leukemias ……….. 61 - 65

Appendix C Top 100 Differentially Expressed Genes B-ALL t(8;14) v normal.. 66 - 72

v

Appendix D Top 100 DEGs for B-ALL without t(8;14) ……………….……… 73 - 76

Appendix E DEGs and Gene Set Enrichment Analysis ……………………….. 77 - 80

Appendix F DEGs with greater than 2-fold change from normal ……………... 81 - 91

Appendix G KEGG pathway analysis on DEGs ……………………………….. 92 - 95

Appendix H Gene Ontology analysis of DEGs ………………………………… 96 - 103

Appendix I Focused analysis of KEGG hematopoietic pathway ……………… 104 - 110

Appendix J Putative modulating microRNAs based on DEG expression …….. 111 - 117

Appendix K Relative ranks of DEGs among 3 ALL groups ……….………….. 118 - 191

Appendix L Master list of DEGs with greater than 2 log-fold change ………… 192 - 220

Appendix M Master list of DEGs found in B-ALL with t(8;14) ………………. 221 - 234

Appendix N Master list of DEGs found in pooled B-ALL without t(8;14) …… 235 - 263

Appendix O Master list of DEGs found in T-ALL ……………………………. 264 - 292

Appendix P Comprehensive KEGG pathway analysis: Biologic pathways …… 293 - 303

Appendix Q Curriculum Vitae of Dr Robert P. Weinberg, DO, JD …………… 304 - 310

vi

LIST OF FIGURES

Figure Description Page

Figure 1 SALL4 expressed in GSE13159 (2,096 samples) …..…………………… 11

Figure 2 SALL4 expressed in the ALL leukemias ……..……………………………. 11

Figure 3 SALL4 expressed in B-ALL with t(8;14) ………………………………….. 11

Figure 4 SALL4 expressed in B-ALL without t(8;14) ………………………………..11

Figure 5 SALL4 expressed in T-ALL leukemia…….…………………………………11

Figure 6 SALL4 expression in AML leukemia .……………………………………….11

Figure 7 SALL4 expressed in CLL leukemia ………………………………………… 12

Figure 8 SALL4 expressed in CML leukemia …………………………………………12

Figure 9 SALL4 expression in MDS …………………………………………………..12

Figure 10 SALL4 expression in healthy cells …………………………………………..12

Figure 11 Scatter plot showing correlation between CD96 and SALL4 ...... ………….. 18

Figure 12 Scatter plot showing correlation between CD36 and SALL4 ...... …………. 18

Figure 13 Scatter plot showing correlation between CD4 and SALL4 ...... ……………18

Figure 14 Scatter plot showing correlation between CD32 and SALL4 ...... ………….. 18

Figure 15 Scatter plot showing correlation between CD2 and SALL4 ..……………..... 18

Figure 16 Scatter plot showing correlation between CD7 and SALL4 ...... ………….. 18

Figure 17 Mean expression of SALL4 in the 8 ALL data sets ………………………… 19

vii

LIST OF TABLES

Table Description Page

Table 1 List of 2,096 leukemia patient samples in GSE 13159 microarray data ...... 10

Table 2 Correlation coefficients: 29 cell surface markers vs SALL4 in ALL ...... ………. 14

Table 3 7 Cell surface markers most highly correlated with SALL4 expression ...... 17

Table 4 35 SALL4-associated cell surface markers in the top 400 correlated genes……... 20

Table 5 Top 300 genes positively correlated with SALL4: r > 0.67 ….……………… 23-28

viii

Acknowledgements

I would like to thank my thesis advisor Li Chai for accepting me as an advisee, even though bioinformatics lie outside her field of expertise. I would also like to thank my thesis co- advisor Rafael

Irizarry for his invaluable suggestions and advice on bioinformatic analysis. I am most thankful to my director Shiv Pillai for his encouragement and mentorship throughout the project and his persevering belief that I could accomplish this. I am also thankful to Michael Carroll for his support and encouragement when things became challenging.

I would also like to acknowledge the following institutions for their software tools used in these genomics analyses: R foundation: R programming language; RStduio: IDE platform; Broad Institute:

GenomeSpace; GenePattern, CytoScape; biomaRt; Perkin Elmer: TIBCO Spotfire; and Advaita Bio for the use of the iPathway tool for KEGG & GO analysis.

I would also like to thank Yunpeng Liu for providing me with invaluable tutoring on programming in R and using Bioconductor. Finally I would like to thank my wife and family for their support and steadfastness in spite of my crankiness and irritability during stressful periods of my research.

This work was conducted with support from Students in the Master of Medical Sciences in

Immunology program of Harvard Medical School. The content is solely the responsibility of the author and does not necessarily represent the official views of Harvard University and its affiliated academic health care centers.

"Success is not final, failure is not fatal, it is the courage to continue that counts..." - Winston Churchill

ix

Chapter 1. Background

1.1. The transcription Factor SALL4

SALL genes (Spalt-like gene family - homologous to a homeotic gene in D. melanogaster) are zinc-finger transcription factors with 4 human isoforms (SALL1, 2, 3, 4) [1]. The SALL4 gene encodes 3 isoforms, called A, B and C, processed by . SALL4 has multiple interactions with different co-factors and also epigenetic complexes [2-5]. Early studies identified SALL4 as an important factor (ESC) for the maintenance and renewal of pluripotency [6-8]. SALL4 has 3 clusters of zinc fingers near the carboxy terminus and one zinc finger at the amino terminus [9] .

SALL4 may form both homo- and heterodimers through interaction of their conserved glutamine-rich region and also contains a nuclear localization signal [10,11]. SALL4 binds to the retinoblastoma binding region 4 (RBBP4), as part of the multiprotein nucleosome remodeling and histone deacetylase complex (NuRD) along with several additional binding partners including

chromodomain-helicase-DNA binding (CHD3/4 or Mi-2a/b), metastasis-associated proteins

(MTA), methyl-CpG-binding domain proteins (MBD2 or MBD3), and histone deacetylases (HDAC1 and

HDAC2) [12,13]. This complex effectively represses transcription through its localization to

heterochromatin. SALL4 also shows transcriptional repressor activity through its binding to other

epigenetic modifiers such as histone lysine-specific demethylase 1 (LSD1), often associated with NuRD

[14]. SALL4 also induces gene activity through its association with the mixed lineage leukemia 1 (MLL) which has histone 3 lysine 4 (H3K4) trimethylation activity [15-18]. SALL4 also binds the

stem cell octamer-binding transcription factor 4 (Oct4) and pluripotency factors Nanog and sex

determining region Y (SRY)-box 2 protein () [19-22]. These complexes affect transcription in

embryonic stem cell regulatory circuits. SALL4 has additional binding partners including the T-box 5

protein (Tbx5), promyelocytic leukemia zinc finger protein (PLZF), and b-catenin downstream

Wnt signaling [23-25]. Some of these binding partners were identified by mass spectrometry or

co-immunoprecipitation.

A key regulatory role for SALL4 involves phophatidyl-inositol-3,4,5-triphosphate

3-phosphatase (PTEN). PTEN is a vital tumor suppressor which induces apoptosis. Through its

binding to the PTEN promoter, SALL4 recruits the NuRD to effect its repression, leading to uncontrolled

proliferation of the cells [17]. SALL4 is important in maintaining the pluripotency of both mouse and

human embryos and the loss of SALL4 results in differentiation of these cells [26-30]. Some of its

activity may be due to the down-regulation of Pou5f1 (encoding Oct4) gene expression along with the

up-regulation of the caudal-type 2 (Cdx2) expression [31,32]. The transcriptional regulatory

network for pluripotency includes SALL4, Oct4, Nanog and Sox2 [6,8,19-22].

SALL4 knock-out mice exhibit congenital malformations of the hand and eyes, which are analogous to the Okihiro/Duane-Radial-ray syndrome in human patients with SALL4 mutation [33,34].

SALL4 is upregulated in several human such as acute myeloid leukemia (AML), B-cell acute 2 lymphocytic leukemia (B-ALL), germ cell tumors, gastric , , hepatocellular carcinoma

(HCC), , and glioma (GBM) [35-50]. SALL4 is associated with worse prognosis and outcome in HCC, metastatic cancers of the endometrium, colo-rectal, and esophageal squamous cell carcinoma. In B-ALL, SALL4 expression is associated with hypomethylation of the intron 1 region [51].

There is direct activation of SALL4 by the signal transducer and activator of transcription 3 (STAT3)

[52]. Canonical Wnt signaling activates SALL4 expression during developmentandincancer [53,54].

When SALL4 is overexpressed in mice, they develop MDS like syndrome and AML [55].

High-risk MDS patients have elevated levels of SALL4 expression [56]. Using short hairpin-RNA to knock down SALL4 expression in leukemia cells or treating with a that mimics the N-12aa of SALL4 result in cell death by apoptosis [57].

1.2. The genetics of B-cell ALL with the t(8;14) translocation

The biology and genetics of acute lymphoblastic leukemias may be found in Appendix F

[references 58-125]. The t(8;14) (q11.2;q32) translocation is relatively rare in B-ALL, but does occur more often in patients with Down Syndrome [126-128]. The ALL patients with t(8;14)(q11.2;q32) are estimated to account for 0.2% of children with B-cell ALL (B-ALL). The prevalence of t(8;14)(q11.2;q32) has been reported as 0.7% of pediatric ALL by the Mitelman database [129]. Burkitt's [104] and B-cell acute lymphoblastic leukemias (B-ALL) [105] are often characterized by a reciprocal chromosomal translocation t(8;14) which involves the immunoglobulin heavy chain on 3 14 [106, 107] with the c- oncogene on chromosome 8 [108-110]. Cloning and sequencing of a Burkitt's lymphoma cell line and pre-B ALL cell line t(8;14) breakpoints have revealed

recombination of the JH region on chromosome 14 with a specific region on chromosome 8 containing homologous signal sequences recognized by the V-D-J recombinase [111-113].

V-D-J recombinase is also involved in the chromosomal translocations in B-cell chronic lymphocytic leukemias (CLL) with the t(11;14) and follicular with t(14;18) translocation

[114, 115]. 80% of Burkittt lymphomas carry a t(8;14)(q24;q32) translocation which results in the juxtaposition of the immunoglobulin heavy chain locus with the MYC protooncogene, resulting in its activation. A smaller number of Burkitt lymphomas display the t(2;8) or the t(8;22) translocations which juxtaposes the MYC gene with the κ or λ immunoglobulin light chains. 85% of follicular lymphomas carry a t(14;18)(q32;q21) translocation resulting in the overexpression of Bcl-2 protein, which has an inhibitory effect on apoptosis [121, 122].

These translocations which result in dysregulation of Bcl-2 produce a more indolent malignancy than those translocations which produce dysregulation of c-MYC. The genetic rearrangement with Bcl-2 presumably occurs during improper DH-JH joiningoftheIgH gene [123, 124].

A prominent feature in these lymphomas as well as numerous other B-cell malignancies, including the follicular lymphomas, acute lymphoblastic leukemias (ALL) and chronic lymphocytic leukemias (CLL), is that the abnormal translocation results in the activation of MYC because the fusion product is under 4 the regulatory control of the immunoglobulin elements [116-118]. There are similar chromosomal translocations involving the T-cell in T-cell malignancies [119, 120]. In each of these cases of translocation-induced malignancies, there is deregulation of an oncogene or putative oncogene which comes under the regulatory control of the juxtaposed immunoglobulin control elements. This mechanism of oncogenesis involves the V-D-J joining recombinases, which normally play an important role in generating receptor variability and diversity in the B-cell and T-cell receptors, acting erroneously in generating chromosomal breakage and translocation.

There was one case report of acute lymphoblastic leukemia with a t(14;18)/BCL2, t(8;14)/cMyc and also t(1;2)/FCGR2B [125]. This case was classified as acute lymphoblastic leukemia

FAB subtype L2 (ALL-L2), which despite immature morphology, the leukemic blasts had a mature B-cell phenotype positive for such B-cell markers as CD19, CD20, HLA-DR and surface immunoglobulin but negative for the more primitive markers CD34, TdT and CD10. Consistent with their mature B-cell phenotype, the cells were also negative for the megakaryocytic marker CD41, myeloid markers CD13 and

CD33, and the T-cell markers CD3 and CD7. In terms of the neoplastic hallmarks, it is believed that the cMYC and FCGR2B would tend to increase cell proliferative capacity, while the BCL2 would tend to inhibit apoptosis.

5 Chapter 2. Data and Methods

2.1 Microarray analysis of Gene expression

Over the past 150 years since Gregor Mendel birthed the field of genetics with his pea breeding experiments [138], genetics has undergone 2 revolutions. In the first revolution, biologists learned to do molecular dissection of the genome utilizing molecular cloning and recombinant DNA methodology cutting, splicing and copying elements of interest with restriction endonucleases [139-142]. In the second revolution, beginning with the Project [143,144] biologists learned to ultra-dissect and map the genome on the sequence level with high-throughput platforms (HTS) and computational methods which has yielded enormous amounts of data on genetic elements during various biological or disease states [145,146]. The field of molecular cell biology has grown exponentially over the past 2 decades with several evolving methodologies including: protein/nucleic acid sequences, single-nucleotide polymorphisms (SNPs), expressed sequence tags (ESTs) , Genome-wide association studies (GWAS), microarrays, RNA-seq, ATAC-seq, whole genome and whole exome sequencing, mass spectrometric proteomics, flow cytometry, and en masse this has birthed the field of computational biology and bioinformatics (the world of -‘omics’) [147-149]. In 1999, Golub et al. demonstrated the clinical utility of performing gene expression profiling on several leukemia datasets [150]. In a similar vein, this thesis has focused on the microarray gene expression data from a large leukemia study with

2,096 patient samples [151]. 6 This study focuses on the analysis of microarray gene expression data obtained using the Affymetrix Gene chip HGU-133 Plus 2 [152], which has the capacity to detect 22,000 genes in the human genome. The detection is based upon DNA purine-pyrimidine hydrogen bonding (A-T, G-C) between immobilized oligonucleotide probes which are single-stranded DNA fragments bound to a solid surface bonding to labeled cDNA fragments which are synthesized from mRNA which has been extracted and isolated from the experimental cells or tissue of interest. Thus the measured intensities on the

Affymetrix Gene chip reflect the amounts of transcribed mRNA in the cells – thus a measure of the transcriptome.

The mRNA was extracted and purified from 2,096 human patient samples which included 18 subsets: 8 genetically distinct groups of acute lymphoblastic leukemia, 6 genetically distinct groups of acute myeloid leukemia, 1 group of chronic lymphocytic leukemia, 1 group of chronic myelogenous leukemia, 1 group of myelodysplastic syndrome, and 1 group of healthy normal subjects.

Initial exploratory data analysis sought novel associations or biomarkers which accompany SALL4 expression in leukemia cells.

2.2 Bioinformatics and Computational Biologic tools

The field of bioinformatics grew from a synthesis of the interdependent fields of molecular biology, computational genetics, statistics, data science and artificial intelligence-driven machine learning and algorithms. The microarray gene expression data was analyzed with the R programming language 7 [153] utilizing algorithms from the Bioconductor platform (www.bioconductor.org). Bioconductor is a computing platform developed for handling the large amounts of data usually generated by

High-Throughput biological assays including microarrays, RNA-seq, ATAC-seq and more.

The Bioconductor computing platform includes over 1,000 software-driven packages for the input, standardization, normalization and statistical analysis of gigabytes of these biological data. For this thesis, we utilized Bioconductor packages for the normalization of data, followed by linear regression analysis looking for correlation among genes in the expression data. In addition, Bioconductor machine learning packages were utilized for hierarchical clustering of the data. Following the data analysis, Bioconductor allows for the output of the results in .csv, .txt, .tsv or Excel files. Bioconductor also provides for the graphic representation of the data which yields the results as output in scatter plots, pie charts, histograms, boxplots and bar plots, among others.

2.3 R programming language and Bioconductor software tools

The data analysis was performed using the programming language R, which is both an object-oriented as well as a functional programming language. It was developed by Robert Gentleman as a derivation of the S programming language which was developed at Bell Laboratories in the 1960s.

The R programming language is an open-source programming language which is freely available to the public for use by anyone at http://cran.r-project.org. R is a commonly used programming language in bioinformatics for the analysis of genomic data, along with Python which is also commonly used. The 8 Bioconductor packages which implement numerous types of statistical and genomic analysis are also open-source, freely available to the public without registration and may be downloaded at http://www.bioconductor.org.

2.4 Data and computational Results

The microarray gene expression data used in this study were obtained from the publicly-available genomics data repository Gene Expression Omnibus (GEO) at the National Center for

Biotechnology Information (NCBI), NIH. The primary study utilized is entitled "An international multi-center study to define the clinical utility of microarray-based gene expression profiling in the diagnosis and subclassification of Leukemia" [125]. This "Microarray Innovations in LEukemia"

(MILE) study includes a cohort of n = 2,096 patient samples data based on the retrospective whole-genome analysis. Table 1 shows the type of leukemia samples and the number of samples per group comprising the microarray gene expression data deposited as GSE13159 in the Gene Expression

Omnibus (GEO) database available online at http://www.ncbi.nlm.nih.gov. Represented in this dataset are 8 groups of acute lymphoblastic leukemia (ALL), 6 groups of acute myeloid leukemia (AML), 1 group of chronic lymphocytic leukemia (CLL), 1 group of chronic myeloid leukemia (CML), 1 group of myelodysplastic syndrome (MDS) and 1 group of normal health samples.

9 Table 1. 2,096 patient samples in GSE13159 microarray gene expression dataset

Leukemia type number of samples 1. mature B-cell ALL with t(8;14) 13 samples 2. Pro-B-ALL with t(11q23)/MLL 71 samples 3. c-ALL/Pre-B-ALL with t(9;22) 122 samples 4. T-ALL 174 samples 5. B-ALL with t(12;21) 59 samples 6. B-ALL with t(1;19) 35 samples 7. B-ALL with hyperdiploid karyotype 40 samples 8. c-ALL/Pre-B-ALL without t(9;22) 237 samples 9. AML with t(8;21) 40 samples 10. AML with t(15;17) 37 samples 11. AML with inv(16)/t(16;16) 28 samples 12. AML with t(11q23)/MLL 38 samples 13. AML with normal karyotype + other abnormal 347 samples 14. AML with complex aberrant karyotype 51 samples 15. chronic lymphocytic leukemia (CLL) 448 samples 16. chronic myeloid leukemia (CML) 76 samples 17. myelodysplastic syndrome (MDS) 207 samples 18. normal healthy bone marrow (normal) 73 samples abbreviations: ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia

Figures 1 – 10 below represent bar charts representing the SALL4 gene expression levels in the various subsets (ALL, AML, CLL, CML, MDS, normal) of GSE13159. Each bar or line segment represents a single patient’s sample in the microarray [some pics stretched vertically to attempt to standardize the y-axis scale to render different pics comparable with regards to scale of SALL4 expression].

10 SALL4 expression in leukemia patient samples: Y-axis = normalized SALL4 expression; X-axis - each bar/column represents a different patient sample

Figure 5. SALL4 expressed in T‐ALL Figure 6. SALL4 expressed in AML leukemias

11 Figure 7. SALL4 expressed in CLL leukemia Figure 8. SALL4 expressed in CML leukemia

Figure 9. SALL4 expressed in MDS Figure 10. SALL4 expressed in healthy cells

As these 10 figures show, each subset is very heterogeneous with regards to the SALL4 expression. We then calculated the mean SALL4 expression for each of the groups. Figure 7 below shows the mean SALL4 expression levels for each of the 8 acute lymphoblastic leukemias. The highest mean level of SALL4 expression is seen in B-cell ALL with t(12;21) with SALL4 = 0.32. The next highest level of SALL4 expression is seen in c-ALL/pre-B ALL with SALL4 = 0.27. The remaining 6

subgroups of ALL have SALL4 levels of expression between 0.09 and 0.17.

12 As one of the aims of this project is to identify cell surface markers which correlate with the

SALL4 levels, we performed a correlation analysis comparing SALL4 expression levels with the expression levels of the other 23,520 gene-probes on the Affymetrix HG-U133plus2 gene chip. We initially selected from the top 100 correlation coefficients for the 29 cell surface markers commonly found in the hematopoietic cell lineage (CD2-CD133) versus SALL4. Looking at the top 100 coefficients, it became obvious that the only subgroup with correlation coefficients greater than 0.60, when correlating SALL4 with cell surface markers, was the mature B-cell ALL with translocation t(8;14) group (column 5 in table 2).

Table 2 below shows the correlation coefficients for the 8 ALL subsets. The remaining leukemia and MDS groups showed correlation coefficients less than 0.40 and these low correlation coefficient values can be seen in Appendix C (6 groups of AML, CLL, CML, and MDS). The 7 cell surface markers with correlation coefficients greater than 0.60 are listed in Table 3 along with their common aliases. Background information on these 7 surface markers are further described in section

2.4.a.

13 Table 2. Correlation coefficients for 29 cell surface biomarkers (CD2 - CD133) vs SALL4 expression in 8 ALL subsets (gleaned from the top 100 SALL4-positive correlates)

As can be seen in Table 2, the only significant correlation coefficients ( | r|> 0.60) comparing SALL4 with cell surface markers CD2 - CD133 occur in the mature B-cell ALL with t(8;14) [B-ALL t(8;14)]. The other seven lymphoblastic leukemia subsets have correlation coefficients of | r | < 0.40 . Setting this finding in perspective, the B-ALL t(8;14) group comprises 13 samples out of a dataset of 2,096 samples. Thus (13/2096) x 100 = 0.62 % of the complete dataset. Not even 1% ! Additional data to be shown shortly reveals that this group does not have a high SALL4 level compared

14 to the other groups. In fact, it has one of the lowest SALL4 levels overall andthelowestmeanSALL4 level. The 7 cell surface markers with correlation coefficients greater than 0.60 are listed in Table 3 along with their common aliases. As seen in Figure 3, the SALL4 expression levels in B-ALL t(8;14) are heterogeneous and vary between 0.03 - 0.24 with a total of 13 samples in the group. With 7 cell surface markers in the B-ALL t(8;14) group with correlation coefficients r > 0.60 comparing with SALL4 expression (Table 3, Figures 11 - 16), we decided to further focus on this group for further assessment. It also made sense to look beyond the cell surface markers and compare the SALL4 correlation with the complete Affymetrix chip of 23,520 gene-probe pairs. The subsequent analyses on this small group of samples introduces signifcant bias and limitations.

2.4.a Background on Cell surface Markers: CD2, CD4, CD7, CD14, CD32, CD36, CD96

Seven of these cell surface biomarkers which correlate well with SALL4 include: CD36, CD32, CD4,

CD96, CD14, CD2 and CD7. CD32 is a B-cell cell surface marker (FCGR2A) [154,155]. CD4 is a cell surface glycoprotein found on T-lymphocytes, monocytes, macrophages and dendritic cells [156-163].

CD96, otherwise known as Tactile ( activation, increased late expression), is a receptor expressed on T-lymphocytes and NK cells [164,165]. CD14 is a cell surface protein which acts as a co-receptor, along with TLR4, for the detection and binding of bacterial lipopolysaccharide (LPS) [166-170]. CD14 was the first pattern recognition receptor described in the literature for the innate .

CD2 is a cell surface adhesion molecule found on T-lymphocytes and natural killer (NK) cells; it is also known as T-cell surface T11/Leu-5, LFA-3 receptor, erythrocyte receptor and rosette receptor [171-173].

CD36 is an integral membrane protein with fatty acid translocase activity (FAT), and is also 15 known as platelet glycoprotein 4 and scavenger receptor class B member 3 (SCARB3). CD36 is found on

the cell membranes of many cell types in vertebrates. It is found on monocytes, platelets, erythrocytes,

endothelial cells, splenocytes, adipocytes, and skeletal myocytes. It is a member of the class B

scavenger receptor family and it binds many ligands including thrombospondin, oxidized low density

lipoprotein and oxidized phospholipids, collagen, plasmodium-infected erythrocytes, long chain fatty

acids and native lipoproteins [174-183]. Studies suggest that CD36 may have a role in atherosclerosis,

fatty acid metabolism, Alzheimer's disease, arterial hypertension, diabetes mellitus, glucose intolerance

and cardiomyopathy. CD7 is a protein in the immunoglobulin superfamily and is found on mature T

lymphocytes and thymocytes. CD7 plays important roles in T-cell/B-cell interactions as well as an

essential role in T-cell function [184-186]. CD7 also interacts with PIK3R1.

These 7 cell surface biomarkers are tabulated with their correlation coefficient in table 3 below. With the appropriate monoclonal antibodies, these cells may be identified, isolated and purified by flow cytometry, immunofluorescence, mass cytometry with tagged antibodies. These 7 cell surface markers were initially found by considering the top 100 positive gene correlations. A total of 35 cell surface markers, with r > 0.64, were discovered by screening the top 400 positive gene correlations.

These 35 cell surface markers with relatively high correlation to SALL4 areseeninTable4.

16 Table 3. 7 Cell surface markers with the highest correlation with SALL4 in B-cell ALLwitht(8;14) [compared with normal (n = 73 samples) and B-cell ALL with t(8;14) (n = 13 samples)]

Cell Surface Marker (+ aliases) Correlation Coefficient (r) CD36 (GP4, GP88, FAT, BDPLT10, SCARB3) 0.77 CD32 (FCGR2A, FCG2) 0.74 CD4 0.73 CD96 (TACTILE) 0.72 CD14 (EntrezID 929) 0.70 CD2 (LFA-2, SRBC, T11) 0.65 CD7 (GP40, LEU-9, TP41, Tp40) 0.60

Scatter plot graphs comparing cell surface biomarkers vs. SALL4 expression

The scatter plots below were computed and plotted using the R programming language using the IDE

RStudio. Each data point represents one of each of the 13 samples of the B-cell ALL with t(8;14) subset; these are plotted in the graphs below in Figures 1 – 6, with the SALL4 expression level plotted on the x-axis and the cell surface biomarker expression plotted on the y-axis. The scatter plots represent the 7 cell surface biomarkers with a correlation coefficient r > 0.60 (listed above in Table 3). The right-upward sloping of each trendline is consistent with a positive correlation.

17 Figure 11. CD96 v. SALL4 Figure 12. CD36 v. SALL4

Figure 13. CD4 v. SALL4 Figure 14. CD32 (FCGR2A) v. SALL4

Figure 15. CD2 v. SALL4 Figure 16. CD7 v. SALL4

18 Figure 17. Mean expression of SALL4 expression in 8 acute lymphoblastic leukemia groups. Expression levels represent the normalized intensity of dot luminosity on Affymetrix chip

In figure 7, we see that the highest level of SALL4 expression is seen in group5:B-cell acute lymphoblastic leukemia (ALL) with t(12;21) translocation (SALL4 = 0.30) with twice the level of expression compared with groups 1 - 4, 6 and 7. The c-ALL/pre- lymphoblastic leukemia (group 8) shows the next highest level of SALL4 expression (SALL4 = 0.28). The remaining 6 ALL datasets: (1) B-cell ALL with t(8;14), (2) B-cell ALL with t(11q23)/MLL,

(3) c-ALL/preB with t(9;22), (4) T-cell ALL, (5) B-cell ALL with t(1;19) andthe(6)

B-cell ALL with hyperdiploid karyotype all have SALL4 expression levels between 0.09 - 0.17.

After finding these 7 surface markers in the top 100 correlated genes in the

B-ALL with t(8;14) subset, we decided to expand the search to look at top 400 gene-probe sets with correlation to SALL4 with coefficients greater than 0.64 and found within this group 35 cell surface markers which are listed in Table 4.

19 Table 4

(For r > 0.64 )

20 Table 4 (continued on next page) Table 4 (continued from previous page)

Some unusual observations can be made when looking at table 4. We must remind ourselves that the subset being examined is a mature B-cell ALL with t(8;14). However we see that several of these cell surface markers are canonical T-lymphocyte markers: CD8B, CD4, CD3e, CD3d, ICOS

(CD278), CD2 and ITGAL (LFA-1). CD2 is commonly expressed on T-cell and NK cells.

ITGAL, or LFA-1, shows increased expression on memory T cells. Meanwhile ICAM4, associated with the erythroid lineage, is upregulated with SALL4. Thus the expression of SALL4 is associated with marked infidelity of the B-ALL with t(8;14) which results in its expressionof T-celland B-cell lymphoid surface markers, as well as erythroid and perhaps NK surface markers. The c-Myc levels have been looked at and c-Myc is generally expressed at a high level across the board with these leukemia

21 subgroups, so that the SALL4 effects seen in the B-ALL with t(8;14) do not appear to be related to the expression of c-Myc.

Tables 3 and 4 satisfied our aim to find cell surface markers which correlate with SALL4 in this ALL leukemia subset of B-ALL with t(8;14). In order to better understand the role and cell biology of

SALL4, we then ran the correlation function looking at the 23,520 gene-probe pairs to find all expressed gene factors which correlate with SALL4. Table 5 represents the top 300 genes which are positively correlated with SALL4.

On superficial inspection, there are some interesting cell surface markers and other coding genes which appear to be relatively highly correlated with SALL4. However, we must consider the limitation that this groups only numbers 13 samples. Furthermore, the group does not have a high SALL4 expression nor does it have a high mean SALL4 expression. Thus it can be concluded that the t(8;14) translocation is not sufficient to account for the SALL4 expression nor its correlations. Out of the 13 samples in the group, approximately one-third have low SALL4 levels, one-third intermediate SALL4 levels and one-third has high SALL4 levels. Statistically it seems that the model we are observing must comprise at least a 2-hit occurrence of independent events, if not a 3-hit process. One of these hits appears to be the SALL4.

However we do not know which is the second or third necessary genetic/biochemical event which is determinative of the high SALL4 levels and its positive correlations.

The c-Myc levels have been looked at and the c-myc levels generally are high across the board for all the leukemia groups. So it appears that the c-myc is not the second required event to satisfy the 2-hit model (or 3-hit model). 22 The differentially expressed genes (DEGs) which are affected by the SALL4 level were calculated statistically in 2 ways. Initially we decided to use the healthy normal cells as controls to look for which genes are differentially expressed in the high-SALL4 state. After this analysis was completed, due to the complex effects of chromosomal translocation, and potential fusion of the c-myc gene with the IgH gene, further reflection suggested using the 3 lowest SALL4 expressors in the B-ALL t(8;14) group as the controls against the 3 highest SALL4 expressors in the group.

There was approximately 80% overlap of the DEGs found using the two different control groups.

The top DEGs are shown in Appendices C, D, E and F, and the comprehensive master list of all DEGs is shown in appendices L, M, N and O. The DEGs with greater than 2 log-fold changemaybeseenin appendix L. After finding the top DEGs, Gene Set Enrichment Analysis was performed. The KEGG pathway analysis is shown in Appendices G and I. The Gene Ontology analysis is shown in appendix H.

An in-depth examination of the effects of the DEGs on the hematopoietic pathway may be seen in appendix I. The comprehensive list of KEGG biologic pathways impacted may be seen in appendix P.

All of these bioinformatics and statistical analyses are based upon 13 samples with varying levels of

SALL4 expressed. To corroborate these alleged effects of SALL4 on the B-ALL t(8;14) leukemia cells, it would be prudent to do biological experiments in the lab either deleting SALL4 or over-expressing

SALL4 in the relevant cells. Because many replicates may be thus performed in the lab, sufficient numbers would potentially validate these findings and overcome the bias of a small number of samples.

Ideally one would find an immortalized cell line, comprising the B-ALL with t(8;14), which mimics the phenomena being studied though this data mining.

23 Table 5. Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

Table 5 is continued on the next page 24 Table 5. (continued from previous page) Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

Table 5 is continued on the next page 25 Table 5. (continued from previous page) Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

Table 5 is continued on the next page 26 Table 5. (continued from previous page) Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

Table 5 is continued on the next page 27 Table 5. (continued from previous page) Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

Table 5 is continued on the next page 28 Table 5. (continued from previous page) Genes with positive correlation to SALL4 in B-ALL with t(8;14)

Gene Symbol r Gene name

29 2.5 Brief Discussion of Results

We began this study with the focus of identifying novel biomarkers and genetic associations with SALL4 in the groups of acute myeloid leukemia. In particular, we began to seek cell surface biomarkers which are correlated with SALL4 in order to have a handle by which to identify, isolate and purify enriched populations of SALL4-positive cells for further research on the cell biology and molecular genetics driven by the transcription factor SALL4 because to the best of the author’s knowledge no one has described or identified any cell surface markers which would identify

SALL4-positive cells (or SALL4Hi cells).

The initial analyses among the 6 subsets of acute myeloid leukemia failed todisclosea correlation between SALL4 and one of the 29 cell surface markers commonly associated with the hematopoietic progenitors (CD2 - CD133). We then performed correlation analyses of the remaining

11 subgroups including the 6 acute lymphoblastic leukemia subsets (ALL, Table 2), the chronic lymphocytic (CLL) and chronic myeloid (CML) leukemic subsets, the myelodysplastic (MDS) subsets

(Appendix A). The correlation coefficients for AML, CLL, CML and MDS all had coefficients less than

0.40. Five of the six ALL subsets also had correlation coefficients less than 0.40 (Table 2). However, in the B-cell ALL subset with t(8;14), while screening the top 100 SALL4-correlated genes we found 7 cell surface biomarkers with correlation coefficients between 0.60 and 0.77. These 7 cell surface biomarkers with relatively high correlation coefficients include CD36, CD32, CD4, CD96, CD14, CD2 and CD7 in 30 order of decreasing correlation coefficient (Tables 2, 3). We then expanded the search for relevant cell surface markers by screening the top 400 SALL4-correlated genes, and we found 35 positively-correlated cell surface markers in this larger group (Table 4). We then ran into a puzzling observation. In this group of 35 SALL4-correlated cell surface markers were several canonical T-cell markers which was surprising to see in a B-cell ALL. The expression of SALL4 appeared to introduce infidelity to the B-cells, which then appeared as a mix of lineages between B-cells and T-cells.

We were at a loss to explain why this one B-cell ALL with t(8;14) should be different from the other 7 groups of ALL studied: pro-B-ALL with t(11q23)/MLL, c-ALL/pre-B-ALL with t(9;22),

T-ALL, B-ALL with t(12;21), B-ALL with t(1:19), B-ALL with hyperdiploid karyotype and c-ALL/pre-B-ALL without t(9;22) in having these 35 SALL4-positive surface markers. As the t(8;14) translocation frequently results in the recombination and fusion of the c-myc and the IgH genes with constitutive expression of c-myc, we questioned whether this played a role in this phenomena. An analysis of the c-myc expression revealed it to be generally elevated across the board for all the leukemia groups.

Perhaps the translocation between 8 and 14 results in the silencing, missense or overexpression of some genes, other than c-myc, which play a role in the biology of SALL4.

Thus the association of this B-cell ALL with t(8;14) with the 35 SALL4-correlated cell surface biomarkers differentiates it from the other 7 acute lymphoblastic leukemia cases, and from the

31 acute and chronic myeloid leukemia subsets as well as from the chronic lymphocytic and myelodysplastic subsets. This posed the possibility that these 35 SALL4-correlated surface markers might be used for theisolation and enrichment of SALL4-positive cells from a heterogeneous mixture of bone marrow or blood cells in patients with B-cell ALL with t(8;14).

However, we must consider the marked limitations and biases inherent in this data mining study.

One limitation involves the relative rarity of the B-cell ALL with t(8;14) among all leukemias. In this

MILE study with a cohort of 2,096 patient samples, there were only 13 samples of the B-cell ALL with t(8;14). This amounts to 0.62 % of the samples studied. Another limitation concerns the fact that not all members of this group had high SALL4 expression. The fact of the varied levels of SALL4 among the 13 samples allowed the discovery of the 35 SALL4-associated cell surface markers. We do not understand the cause of this variation in SALL4 expression. We have speculated that this phenomena may be the result of a 2-hit or 3-hit occurrence involving concurrent independent events similar to models of chemical carcinogenesis involving 2-hits. In such a model the SALL4 expression would result from the cooperative or facilitating effects of the concurrence of the 2 or 3 events. In the end, any data mining findings must be corroborated biologically in the laboratory which is perhaps one of the largest limitations of this study. For these reasons, the discovery of these 35 SALL4-associated cell surface markers must be taken with caution.

32 3. Chapter 3 Discussion and Perspectives

There are several lines of experimental evidence which suggest that the B-ALL with t(8;14) is distinct from the other B-ALL, T-ALL, AML, CLL, CML and MDS leukemia groups found in the MILE study by Kohlmann et al. deposited in the GEO database as

GSE13159. These multiple lines of experimental evidence derive from the computational analysis of the microarray gene expression data which seem to differentiate the B-ALL t(8;14).

First, the B-ALL with t(8;14) is the only group which has 35 cell surface markers positively correlated with SALL4 with coefficients greater than 0.60 (Tables 2, 3 and 4;

Figures 1 - 10). The first seven of these markers were discovered when screening the top 100

SALL4-correlated genes (CD36, CD32, CD4, CD96, CD14, CD2 and CD7). After expanding the search to look at the top 400 SALL4-correlated genes, a total of 35 cell surface markers were identified with correlation coefficients greater than 0.60. The other 16 leukemia/myelodysplastic groups have correlation coefficients less than 0.40 comparing the

SALL4 gene with the surface markers.

Linear regression was used to find the differentially expressed genes in these groups. As with all experiments, the proper choice of controls is essential for validity and interpretation of experimental results. Using the R/Bioconductor platform we made two different calculations of the differentially expressed genes (DEGs). The first set of calculations involved comparing the B-ALL t(8;14) with the healthy normal cells as controls, finding which genes are differentially expressed from the normal cells. The second set of calculations was made completely within the B-ALL t(8;14). The 3 highest SALL4 expressing samples were compared with the 3 lowest SALL4 expressing samples as controls. When looking at the

DEGs calculated using these 2 different controls, there is approximately 80% overlap of the

DEGs found.

33 In addition to the R/Bioconductor platform, we also used the iPathway tool available through Advaita Bio. The iPathway tool generated a total of 1,802 DEGs for the 3 ALL groups: B-ALL t(8;14), pooled B-ALL without t(8;14), and T-ALL. The iPathway tool generated 549 DEGs for the B-ALL t(8;14) group.

We then performed functional genomic analysis and Gene Set Enrichment Analysis on the 1802 differentially expressed genes (DEGs) comparing the acute lymphoblastic leukemia groups (B-ALL and T-ALL) with the healthy normal using linear regression (Appendices E -

J). We see that B-ALL with t(8;14) has 81 unique DEGs not shared with the otherALL groups (Appendix E). The pooled B-ALL groups (without t(8;14)) have 416 unique DEGs, and the T-ALL group has 435 unique DEGs not shared with the other groups. The DEGs may represent the "fingerprint" of each dataset and this distribution of DEGs shows that the

B-ALL with t(8;14) has a distinct and unique fingerprint separating it from all the other leukemia / myelodysplastic datasets.

We then performed Gene Ontology analysis of the DEGs expressed by the B-ALL with t(8;14) (Appendix H). The GO analysis yields Immune system response, Immune response,

Defense response, response to bacterium and response to external biotic stimuli as the top biological processes evident in the DEGs. GO analysis also yields carbohydrate binding, immunoglobulin receptor binding, phenanthrene 9, 10-monoxygenase, other oxido-reductases, glycosaminoglycan binding, CXCR chemokine receptor binding and chemokine receptor binding as the top molecular fundtions evident in the DEGs. Finally the GO analysis yields the extracellular space, vesicles, membrane-bound vesicles, cell periphery and the plasma membrane as the top cellular components. The Gene Ontology of the B-ALL t(8;14) is quite distinct from the Gene Ontology of the other B-ALL leukemias and T-ALL.

The fourth line of evidence comes from the KEGG pathway analysis of the DEGs expressed by B-ALL with t(8;14) (Appendix G). KEGG pathway analysis reveals the top biological pathways in B-ALL with t(8;14) to be: hematopoietic cell lineage; porphyrin 34 metabolism; amoebiasis; leishmaniasis; asthma; transcriptional dysregulation in cancer; cell adhesion molecules; nitrogen metabolism; cytokine-cytokine receptor interactions; and Th17 cell differentiation. The KEGG pathway analysis shows some overlap of pathways among the

ALL leukemias, but the B-ALL t(8;14) has a distinct priority of pathways.

The fifth line of evidence comes from an analysis of the putative microRNAs involved with the modulated expression of DEGs in B-ALL with t(8;14) (Appendix J). Ofnote,we see that over 90% of the putative microRNAs predicted to modulate the expression of DEGs belong to the B-ALL with t(8;14) group, whereas fewer than 10% of the putative microRNAs modulate DEG expression in the other B-ALL and T-ALL groups.

The sixth line of evidence supporting the distinct nature of the B-ALL t(8;14) group comprises the relative ranking of DEGs among the 3 ALL groups (Appendix K).

3.4 Limitations

As discussed above, there are multiple limitations to this study and the conclusions which may be drawn from the bioinformatic analyses. First and foremost is the fact that these findings have not been corroborated by biological validation in the laboratory, which would probably take the form of SALL4 deletion or overexpression in cells.

Statistically the study is quite biased because of the small number of samples involved.

The B-ALL t(8;14) group only comprises 0.62 % of the 2,096 sample dataset. Furthermore only one-third of this group, or 0.2 % of the total dataset, comprises high SALL4 expressing cells. That is 3 samples of this B-ALL t(8;14) are SALL4hi out of 2,096 leukemia samples.

We conjecture a model whereby the phenomena and observations made in this study are highly dependent upon the concurrence of 2 or 3 independent genetic/biochemical events, and

SALL4 is not dominant-determinative in the Mendelian sense. Similar to the failure to detect the phenotype of a recessive trait without the homozygous condition, these phenomena likewise require the simultaneous expression of at least 2 events and we are only cognizant of the SALL4 expressing event. 35 3.3 Future Research Paths

The results of this study may allow us to anticipate the biological effects of SALL4

in the appropriate experimental model. If these findings are valid, we may look for the

expression of some of the 35 identified cell surface markers during the overexpression of

SALL4 in the relevant cells and loss of these markers with deletion of SALL4.

It would be ideal to have a cell line which is genetically and biochemically similar

to the B-ALL t(8;14). Perhaps such a cell line may be propagated from a sampleof

B-ALL t(8;14) in one of the growing Biobanks.

If a sufficient number of samples of the B-ALL t(8;14) can be gathered given the

relative rarity of this type of leukemia, RNA-seq analysis may allow corroboration of

these findings as well as further investigation into the biology and mechanisms by which

SALL4 causes some of these phenomena.

Finally since SALL4 is a transcription factor, some ChIP-seq experiments would be

helpful to explore the promoters, enhancers and insulators affecting the expression of

SALL4 downstream effects. The author searched the GEO database for ChIP-seq

experiments with SALL4 but could only find 2 such studies. A major limitation

regarding ChIP-seq experiments is that currently there are no good monoclonal

antibodies against SALL4 and it may not be very immunogenic.

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57

Appendix A

Correlation coefficients for cell surface markers vs. SALL4 in: AML CLL CML MDS

58

Appendix A: Very weak or null correlating surface markers with SALL4

Table A1 SALL4 Correlation coefficients with surface markers for 6 AML datasets ( | r | < 0.45)

Tables A1 (above) and A2 (below) show that the remaining 9 datasets comprising: AML (6 data sets), CLL (1 dataset), CML (1 dataset) and MDS (1 dataset) do not have any gene correlations with

| r | > 0.40 for the 29 cell surface markers CD2 - CD133 vs. SALL4. Thus the B-ALL with t(8;14) subset is unique among the 16 leukemia and 1 MDS subsets because of the multiple surface markers which are correlated with SALL4 with coefficient r > 0.70.

59

Table A2. SALL4 Correlations for CLL, CML and MDS datasets ( | r | < 0.32)

All of the correlation coefficients in table 5 are | r | < 0.32 for the 731 samples in the CLL, CML and MDS subsets from GSE13159 which suggests that there is no positive correlation between the 29 surface biomarkers vs. SALL4 with any of these 3 data subsets.

60

Appendix B

Biology and Genetics of the

Acute Lymphoblastic Leukemias

61

Biology and genetics of the Acute Lymphoblastic Leukemias

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer [58]. In 1948,

Sydney Farber and Yellapragada Subbarow showed that remission from ALL could be induced in children with aminopterin, a folate antagonist, making it one of the first cancers to be treated with chemotherapy and thus rendering Sydney Farber the father of modern cancer chemotherapy [59]. Over the past 70 years, enormous strides have been made in the treatment of pediatric leukemia providing its patients with some of the best prognoses of all the known cancers. However, in spite of this success, ALL continues to cause significant morbidity and mortality in children, in part due to the genetic and biological heterogeneity of the disease [60].

ALL is a cancer of the lymphoid lineage of blood cells, characterized by overproduction and accumulation of immature lymphoblasts in the bone marrow along with their infiltration into peripheral organs [61]. This accumulation of lymphoblasts in the bone marrow inhibits the normal development of the erythroid lineage (red blood cells), the myeloid lineage (neutrophils, eosinophils, basophils) and the thromboid lineage (platelets). The resulting neutropenia and anemia cause multiple infections, fatigue, fever, hemorrhage and organ dysfunction due to ischemia and increased anaerobic metabolism [60].

The genetics of ALL is complex reflecting the heterogeneity of the disease [58]. Familial ALL may be caused by the inheritance of a germline mutation of PAX5, ETV6 and CDKN2A most often [62-

65]. Genome-wide association studies (GWAS) in the population have shown that ALL may be caused by single nucleotide polymorphisms at ARID5B, IKZF1, CEBPE, PIP4K2A, GATA3 and CDKN2A among other loci [66-70]. Some common genetic mechanisms underlying the generation of ALL include altered

DNA damage repair, constitutive enzyme activation by a fusion gene, and dysregulation of some proto- oncogene through its translocated juxtaposition with the promoter of another gene [58]. ALL is 62

commonly found in populations with high ionizing radiation exposure, such as the Hiroshima atomic bomb survivors, as well as exposure to chemical carcinogens including some cancer chemotherapeutics

[71,72]. ALL can easily be studied in the laboratory because the abnormal lymphoblasts and hematopoietic progenitor cells are easily obtained through a bone marrow biopsy, which also provides the definitive diagnosis of ALL in patients.

ALL may affect either the B-cell or T-cell lymphocyte lineages and comprise numerous genetic subtypes involving either somatic chromosomal structural DNA rearrangements (such as translocations, inversions, deletions, duplications, insertions, aneuploidy), copy number alterations, or gene mutations which perturb cytokine receptors, kinase and Ras cell signaling, tumor suppressors, proto-oncogenes, epigenetic chromatin modifiers (such as histone [de]acetylases, [de]methylases) or lymphoid transcriptional programs [58]. B-cell ALL (B-ALL) is more common than T-cell ALL (T-ALL). Some of these ALL subtypes are very amenable to chemotherapy and promise an excellent prognosis, whereas other subtypes are drug-resistant and have a dismal prognosis. Thus proper classification into the appropriate subgroups is vital for prognosis and treatment.

Infants under age 1 year may have a KMT2A (MLL) rearrangement, often with the t(4;11)(q21;q23) translocation, which has a poor prognosis [73,74]. 25% to 30% of pediatric ALL patients may have a high hyperdiploidy with gain of at least 5 chromosomes and the ETV-RUNX1, occurring in less than 3% of young adults, having a favorable prognosis. The BCR-ABL1 fusion seen in

Philadelphia chromosome (Ph)-positive ALL only comprises 2-5% of pediatric ALL whereis it accounts for 25% of adult ALL, and has seen marked improvement in outcomes with the advent of the tyrosine kinase inhibitors (TKIs). The TCF3-PBX1 fusion seen with the chromosomal translocation t(1;19)(q23;p13) occurs in about 5-6% of B-ALL and has a more favorable outcome than the t(17;19)

(q23;p13) translocation which may result in a TCF3-HLF fusion [75,76]. Older children more commonly have complex intrachromosomal amplification of chromosome 21 (iAMP21) which is

63

associated with a poor prognosis [77]. 2-3% of ALL patients may have hypodiploidy with less than 44 chromosomes, with a poor prognosis [78]. There are several distinct subtypes of hypodiploid ALL including near-haploid cases (24-31 chromosomes) showing Ras-activating mutations, IKZF3 or IKZF2 alterations, or TP53 mutations, which are inherited frequently [79].

B-ALL is often characterized by secondary DNA gains, deletions and mutations, which may alter cell cycle regulators and tumor suppressors (CDKN2A/CDKN2B, RB1), lymphoid transcriptional factors

(IKZF1, PAX5, EBF1), regulators of apoptosis, transcriptional regulators (ETV6, ERG) and epigenetic modifiers [80]. These secondary DNA changes vary with the different ALL subtypes. KMT2A-rearranged subtypes rarely show secondary DNA changes [74]. A hallmark of the BCR-ABL1-positive and Ph-like

ALL is IKZF1 alterations, which have a poor prognosis [81-85]. Hypodiploid ALL is characterized by selective mutation of other members of the IKAROS transcription factor family, IKZF2 and IKZF379.

Often in high hyperdiploid ALL, the secondary DNA changes will target genes in the Ras signaling pathway and epigenetic chromatin modifiers [86].

The BCR-ABL1-positive ALL (Ph-like) is genetically characterized by: alterations of the B- lymphoid transcription factors (e.g. IKZF1), cytokine receptor and tyrosine kinase signaling (e.g. alterations of CRLF2, ABL-class tyrosine kinase genes, JAK2, JAK-STAT signaling, Ras signaling

(NRAS, KRAS, PTPN11, NF1) along with less common kinase alterations (e.g. FLT3, NTRK3, BLNK,

TYK2, PTK2B) [87-89].

Another B-ALL subtype is characterized by dysregulation of the double homeobox 4 gene

(DUX4) along with the ETS transcription factor gene (ERG) [90-93]. DUX4 is not normally expressed in B-cells and it encodes a double homeobox transcription factor which is found in a macrosatellite D4Z4 repeat on the long arm of chromosome 4. Translocations of DUX4 may result in DUX4-IGH or ERG-

DUX4 fusion genes [94-96]. There are intragenic deletions of ERG in 5% of childhood ALL patients.

DUX4 rearrangement appears to be an early initiating event in leukemogenesis. 64

Another B-ALL subtype comprises rearrangements of the myocyte enhancer factor 2D (MEF2D) and the zinc finger 384 (ZNF384) [97-100]. There may be MEF2D rearrangements to BCL9,

HNRNPUL1, SS18, FOXJ2, CSF1R and DAZAP1. The BCL9 is the most common rearrangement, and these fusions commonly preserve the MADS-box domain of MEF2D which mediates DNA binding and enhances MEF2D transcriptional activity, which events are transforming and leukemogenic both in vitro and in vivo [101-103]. ZNF384 rearrangements often involve the fusion to a 5’ partner gene, which is often an epigenetic chromatin modifier or a transcriptional regulator such as EP300, CREBBP, TAF15,

SYNRG, EWSR1, TCF3 and ARID1B [102]. These fusions often result in upregulation of the JAK-STAT pathway and are diagnosed as B-ALL with expression of myeloid antigens, which suggests the transformation of a hematopoietic progenitor with B-lymphoid/myeloid potential [103].

We still have a poor understanding of the molecular biology of leukemia which would provide a unified basis for understanding the disease. The heterogeneous genomics of these B-ALL subtypes points to the importance of having good experimental models of B-ALL for the laboratory investigation of the key molecular and genetic events which underlie leukemogenesis in acute lymphoblastic leukemia and may reveal potential therapeutic targets.

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Appendix C

Differentially Expressed Genes (DEGs)

Top 100 DEGs comparing B-ALL t(8;14) with healthy

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Top 100 genes differentially expressed comparing B-ALL t(8;14) with normal cells (using the limma / eBayes / topTable functions for linear regression analysis)

(Genes 61 – 99 are continued on the next page)

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Table 6 (continued) Genes 61 - 99

Background on the top 100 DEGs in B-ALL with t(8;14) vs. normal

In the above table, the first ranked gene, TPT1, encodes the 20-25 kDa "Translationally controlled Tumor Protein" (TCTP), which maps to 13q12q1413 in chromosome 13 [187-189]. hTCTP is a growth-related, calcium-binding protein with multiple functions [190]. It was first discovered in 1989 as a cDNA sequence which was extracted from a human mammary carcinoma. Overexpression of TCTP in HeLa cells inhibits etoposide-induced apoptosis by inhibiting caspase-3 like activity [191]. TCTP functions in tumor reversion and development

[192,193]. TCTP appears to regulate the cancer stem cell compartment [194] as well as 68

regulates tumor reversion [195]. TCTP is a pro-survival protein which antagonizes BAX function [196].

The 7th ranked HNRNPA1 belongs to the A/B subfamily of heterogeneous nuclear ribonucleoproteins and play a role in pre-mRNA and mRNA processing [197]. Experimentally

HNRNPA1 has been shown to interact with IKBα [198], BAT2 [199] as well as Flap structure-specific endonuclease 1 [200]. Mutations in hnRNP A1 are associated with multisystem proteinopathy and amyotrophic lateral sclerosis.

The 27th ranked gene is DDX5 encodes the RNA helicase p68, also known as the

ATP-dependent RNA helicase DDX5 of the DEAD box protein 5. The DEAD box RNA helicases are involved with modifications of RNA secondary structure, thus affecting the initiation of translation, mitochondrial and nuclear splicing, and spliceosome and ribosome assembly. These enzymes are frequently involved in spermatogenesis, embryogenesis along with cell growth and proliferation [201-205].

The 28th ranked gene OAZ1 is involved in the regulation of bone morphogenetic protein signaling through Smad6 activation [206]. BMP4 stimulation causes an OAZ-Smad1/4 complex to bind to and activate Smad6, a specific inhibitorof the BMP pathway. The TGFβs typically bind to and activate cell surface receptors with tyrosine kinase activity, which subsequently phosphorylate R-Smads (Smad1, Smad5, Smad8) which localize to the nucleus and bind transcription factors which then induce promoter specific transcription. Structurally similar to the

OAZ is the Early Hematopoietic Zinc Finger protein (EHZF, or Evi3 or ZNF521) as both have

30 Kruppel-like zinc fingers with homology of 64%. The OAZ or EHZF has key roles in hematopoiesis [207-212]. 69

The 48th ranked gene is JTB which encodes the "jumping translocation breakpoint protein", also called the "prostate androgen-regulated protein" (PAR). The family of JTB proteins comprise a group of jumping translocation breakpoint proteins which may cause a jumping translocation - an unbalanced translocation which comprises amplified chromosomal segments jumping to potential telomeres [213-216]. These proteins are associated with some carcinomas.

The 51st ranked gene TMSB4X encodes thymosin beta-4, which is an actin-sequestering protein which regulates actin polymerization and as such is involved in cell proliferation, migration and differentiation [217- 219]. Through protein moonlighting, thymosin beta-4 also plays a role in tissue regeneration, cell migration, stem cell maturation, blood vessel formation and reduction of pro-inflammatory cytokines [220-223]. Thymosin β4 also induces the activity of terminal deoxynucleotidyl transferase (TdT) in thymocytes [224]. It also interacts with

ACTA1 and ACTG1 (ACTG1 being the 52nd ranked gene) [225,226].

HLA-A is the 55th ranked gene differentially expressed from normal. Prior studies have shown an association between adult T-cell leukemia and HLA-A26 and HLA-A68 [227].

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is the 61st ranked gene. It plays a key role in glycolysis, initiation of apoptosis, transcription activation, ER to Golgi shuttling, axoplasmic transport [228-237]. The cysteine residue C152 located at the enzyme's active site is essential for the induction of apoptosis by oxidative stress. The multiple moonlighting functions of GAPDH are performed by the cytoplasmic GAPDH which has undergone post-translational modifications. GAPDH can activate transcription as part of the OCA-S transcriptional coactivator complex with lactate dehydrogenase 8 When the GAPDH is S-nitrosylated by NO in

70

response to cell stress, it binds to the protein SIAH1, a ubiquitin ligase, and moves into the nucleus where the Siah 1 targets nuclear proteins for degradation, leading to apoptosis.

The 60th ranked gene is MYL12B, which encodes the regulatory light chain myosin 12B

(also known as MLC-B and MRLC2). The non-muscle myosin II activity is regulated by the phosphorylation of a regulatory light chain, such as MYL12B. The phosphorylated MYL12B has higher ATPase activity and facilitates the assembly of myosin II filaments [238,239].

MRFAP1 is the 68th ranked gene. The MORF4 family-associated protein 1 has been shown to interact with the Retinoblastoma protein and with MORF4L1 [240,241]. LUZP6, the

70th ranked gene, is the leucine zipper protein 6, which is a 6.4 kDa tumor antigen associated with myeloproliferative disease [243-245]. MPD6 is among a group of cryptic antigens and was identified by screening a cDNA library from the testis of 3 patients with polycythemia vera who responded to IFN-α. MPD6 does not have a conventional genomic structure and is encoded by a cryptic open reading frame in the 3'-untranslated region of myotrophin mRNA. SUMO2 is the 72nd ranked gene. SUMO2 encodes a small ubiquitin-like modifier which is involved with apoptosis, transcriptional regulation and nuclear transport [246-266].

H2AFZ is the 75th ranked gene and it encodes histone H2A.Z, a replication-independent member of the H2A histone family. Histone H2A.Z is essential for embryonic development and its deletion/mutation leads to embryonic lethality [267- 270]. H2A.Z also plays a role in repressing a subset of ncRNAs, derepressing CUTs and mediating higher order chromatin structure formation.

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GNG5 is the 82nd ranked gene differentially expressed. It encodes the gamma-5 subunit of the guanine nucleotide-binding protein G(I)G(S)/G(O) which has been identified in CD34+ hematopoietic stem/progenitor cells [271-273]. ARPC2 is the 88th ranked gene and it encodes subunits of the Arp2/3 protein complex which is implicated in the regulation of actin polymerization in cells. ARPC2 maps close to the BRCA2 gene [274] and its reduced expression is associated with human gastric cancers [275].

UQCRH is the 91st ranked gene. It encodes the mitochondrial cytochrome b-c1 complex subunit 6, which is a subunit of the Ubiquinol cytochrome c Reductase (cyt c1 Hinge protein).

Reports suggest altered expression of UQCRH in some cancers [276, 277]. TALDO1 is the 95th ranked gene. It encodes transaldolase which is involved in the pentose phosphate pathway and also regulates apoptosis. The pentose phosphate pathway plays a key role in nucleotide synthesis and the generation of NADPH [278-280], important in proliferating cells.

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Appendix D

Top 100 DEGs for pooled B-ALL without t(8;14)

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Table D1. Top 100 DEGs expressed comparing the other 5 ALL subsets (pooled B-ALL) with normal cells

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Table D2. 82 DEGs, which are shared in common with both the B-ALL t(8;14) subset and the other ALL subsets

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Unique gene sets, differentially expressed by either the B-ALL t(8;14) subset or by the other ALL subsets, but not shared in common (taken from top 100 genes by linear regression)

Table D3. 14 Unique Genes only differentially expressed by B-ALL t(8;14) [not expressed by the other 7 ALL datasets]

1. ANP32B 6. UQCRH/UQCRHL 10. MRFAP1 2. COX7A2 7. USMG5 11. MYL12A/B 3. COX8A 8. EIF4A1 12. NDUFB9 4. GNG5 (SNORA48) 13. TALDO1 5. H2AFZ 9. MMADHC 14. TMSB4X

Concerning these 14 genes in table 9 which are only differentially expressed in the

B-ALL with t(8;14), the following genes have special notes about their cancer-associated properties described on pp. 31 - 33: GNG5, H2AFZ, UQCRH, MRFAP1, MYL12B,

TALDO1 and TMSB4X

Table D4. 15 Unique Genes only differentially expressed by the other 7 ALL subsets [not expressed by the B-ALL with t(8;14)]

1. EEF1A1 6. PFDN5 11. RP3-486E24.1 2. HMGB1 7. PTGES3 12. RPL3 3. HSP90AA1 8. SNRPD2 13. RPL8 4. MORF4L1 9. UBB 14. RPS10/10L 5. NACAP1 10. YWHAQ 15. SARAF

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Appendix E

DEGs and Gene Set Enrichment Analysis

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Gene Set Enrichment Analysis and Functional Pathway analysis

The B-cell ALL with t(8;14) subset was selected for more intensive study because of its unique set of SALL4 with relatively higher correlations to specific cell-surface markers, which was not present in the other leukemia subsets. For the purpose of the analysis of differentially expressed genes (DEGs), a minimum log fold change of expression with an absolute value of at least 2 and a threshold statistical significance level of p < 0.05 was used. The DEGs were then analyzed functionally in the context of pathways obtained from the Kyoto Encyclopedia of

Genes and Genomes (KEGG), gene ontologies based in the Gene Ontology consortium database, miRNAs from the miRBase and TARGETSCAN databases. Diseases were functionally identified from the KEGG database. Utilizing these criteria, 549 DEGs were identified out of a total of 20388 genes with measured expression, which significantly impacted 34 functional pathways. Gene set enrichment analysis showed significant enrichment of 1431 Gene Ontology

(GO) terms, 237 miRNAs, and 57 diseases based on the uncorrected p-values.

Table E1. Summary of Gene Set Enrichment Analysis (B-ALL t(8;14) v. normal)

DEGs 549 genes/20388 Functional Pathways (IPA) 34 Gene Ontology term enrichment 1431 miRNA enrichment 237 Disease-related enrichment 57

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DEGs from iPathway Analysis tool

Differentially Expressed Genes among Leukemia datasets

Figure E1. a) Volcano Plot shows 549 significantly differentially expressed genes (DEGs) which are plotted by their measured expression change (x-axis) versus the significance of the change (y-axis) in terms of the negative log of the p-value, resulting in more significant genes are plotted higher on the y-axis. Red lines represent the threshhold values to select DEGs: 2 for expression change and 0.05 for significance. b) Pathways perturbation versus over- representation: the top 5 pathways are plotted in accordance with over-representation parameter (pORA) on the x-axis and a pathway accumulation parameter (pAcc) on the y-axis. Each pathway is represented by a red circle and the diameter of the circle is proportional to the size of the pathway it represents.

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Gene Expression Profiling of 3 ALL leukemia subsets

Unique versus shared DEGs among: B-ALL t(8;14), pooled B-ALL without t(8;14), T-ALL

Figure E2. Venn Diagram showing the distribution of the DEGs in 3 subsets of ALL (DEGs listed: Appendix A for B-ALL with t(8;14), Appendix B for B-ALL, Appendix C for T-ALL)

ALL subset Unique DEGs B-ALL with t(8;14) 81 pooled B-ALL 435 T-ALL 416

In figure E2, we see that there are 435 DEGs which are uniquely expressed in the pooled

B-cell ALL subsets (without the t(8;14)) vs normal cells, 416 DEGs which are uniquely expressed in the T-cell ALL subsets vs. normal, but only 81 DEGs which are uniquely expressed in the B-ALL with t(8;14) vs. normal. Thus the B-ALL with t(8;14) has a unique gene expression profile which differs from the pooled B-ALL without t(814) or the T-ALL by amuch smaller subset of unique DEGs.

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Appendix F

DEGs with greater than 2-fold change compared

with healthy normal

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Table F1. Top 100 DEGs with greater than +2 log-fold-change

B-ALL with t(8;14) Pooled B-ALL without t(8;14) T-ALL Gene Log FC Gene Log FC Gene Log FC PEG10 4.758648 CSRP2 6.735985 TSPAN7 5.769556 UCHL1 4.28351 PXDN 6.335653 HHIP-AS1 5.583755 TCL1A 4.151425 PSD3 6.187163 CHI3L2 5.381683 BLK 4.06095 ZNF423 5.752159 ARPP21 5.072441 IGF2BP3 3.842438 CD19 5.705484 HHIP 5.011948 ZNF385B 3.820518 EBF1 5.648731 ELOVL4 4.991321 FCRLA 3.795756 DNTT 5.610306 CTHRC1 4.929598 CD19 3.782773 VPREB1 5.523951 CD1E 4.892324 RUBCNL 3.55742 ECM1 5.376437 MLLT11 4.891101 RASSF6 3.555035 CTGF 5.232749 SH2D1A 4.875425 DTX1 3.541892 NPY 5.231316 CD1B 4.638869 LOC10050550 MCOLN2 3.514334 STK32B 5.151912 1 4.59039 RGS16 3.495511 TCL1A 5.057323 DNTT 4.539817 CNR1 3.481988 LINC01013 5.054765 MYO7B 4.531388 HRK 3.457322 LOC100130458 5.040505 NREP 4.489502 PCDH9 3.450646 VPREB3 5.028716 H2BFXP 4.419126 ID3 3.430575 MACROD2 4.919058 TOX 4.38638 CD79B 3.425172 BLNK 4.900857 CRNDE 4.333642 AEBP1 3.284496 SOCS2-AS1 4.87385 CASC15 4.331409 EML6 3.212894 RAG1 4.848365 IKZF2 4.223461 SGCE 3.161312 SHANK3 4.798806 KCTD1 4.166392 CD79A 3.142616 COL5A1 4.760098 PCAT18 4.164637 CLECL1 3.106726 SOCS2 4.668747 SHISA2 4.151406 BTNL9 3.073888 S100A16 4.610645 SOX4 4.05986 IFNLR1 3.063472 MYO5C 4.564428 CD3D 4.015426 BEST3 2.968507 MME 4.533874 ZBTB8A 4.012867 ST14 2.916348 JUP 4.503304 FAT1 3.996821 P2RX5 2.874889 HMHB1 4.462488 ADA 3.966058 PAX5 2.818946 ZCCHC7 4.451519 SCAI 3.95748 SIT1 2.81121 PAX5 4.394562 PEX5L 3.935451 CAMK2D 2.787221 NAV1 4.319883 CD3G 3.925796 CD72 2.780272 STAG3 4.30085 ZNF827 3.887203 FCRL2 2.753409 CYGB 4.275507 CDK6 3.841712 AMN 2.751735 ID3 4.225248 IRX5 3.804387 POU2AF1 2.732874 ARPP21 4.19672 MAP1A 3.795102 GPM6A 2.700947 CMTM8 4.191228 LCK 3.761195 IGHM 2.672221 TCF4 4.162106 LEF1 3.759937 PRDM15 2.669274 SDK2 4.144844 LDOC1L 3.722309 OSBPL10 2.666715 ZNF608 4.121751 PARD3 3.705928

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E2F5 2.585541 DPEP1 4.062542 PRTFDC1 3.679554 BCAS4 2.583105 BLK 4.036841 AEBP1 3.673966 SPIB 2.575843 LEF1-AS1 4.021419 DBN1 3.662343 CD22 2.53516 RASD1 3.995099 GXYLT2 3.621757 LOC10012944 7 2.532964 CLIP2 3.949653 FXYD2 3.606268 BMP7 2.526301 CRIM1 3.941781 SCD5 3.605321 ELL3 2.511824 CD72 3.928398 RUFY3 3.587449 BEND4 2.506533 WFS1 3.84292 TRAT1 3.577729 RAB30 2.482699 RGL1 3.840368 CEP70 3.566104 RNFT2 2.453541 GPSM1 3.82878 MYEF2 3.524716 EBF1 2.443016 AEBP1 3.826969 9/6/2017 3.519171 IKZF2 2.435515 POU4F1 3.822557 ZNRF1 3.505923 DMD 2.396082 CD200 3.809214 ZNF711 3.494332 LOC10106039 BLNK 2.374893 TNFSF4 3.794926 1 3.468019 PAWR 2.365784 FAM69B 3.789159 LRP12 3.465851 CADM1 2.363725 AUTS2 3.782747 CD1A 3.458135 MTCL1 2.363315 CD22 3.776326 PTPRD 3.380072 SH2B2 2.31608 S100A13 3.733369 SCN3A 3.373209 ZNF827 2.302336 MEF2C 3.73182 CAMK4 3.349697 GJC1 2.298816 CD79A 3.729215 CACNB3 3.348082 TCF4 2.295735 CD79B 3.707795 MSI2 3.346897 ZNF608 2.276909 FLT3 3.699143 ZAP70 3.341506 MAPK12 2.243764 FAM129C 3.670972 LINC01260 3.302289 SLC2A6 2.243486 HLA-DOA 3.668849 UMODL1 3.288772 FADS3 2.242744 LEF1 3.648044 CLIP3 3.281194 PMAIP1 2.213932 CIITA 3.617458 BAHCC1 3.272305 FAM129C 2.20128 NT5E 3.613406 ZNF57 3.264928 MS4A1 2.192436 DBN1 3.610414 PIK3C2B 3.253931 MYC 2.172987 FADS3 3.600531 CD7 3.239652 OAS1 2.171425 SSBP2 3.592255 EPHB6 3.212509 SEL1L3 2.167389 SOX4 3.560516 SBK1 3.201693 SMARCA4 2.156194 ZNF704 3.549638 CHRNA3 3.179606 TRAF5 2.142813 AFF3 3.541537 FAM110C 3.169374 MARCKSL1 2.123991 KCNK12 3.520033 ABI2 3.162569 HLA-DOB 2.120855 BCL7A 3.490274 HES4 3.139823 HLA-DPA1 2.116471 CD34 3.482346 MZB1 3.136116 GPR18 2.10365 FAM150B 3.470401 PLCG1 3.104166 PPFIBP2 2.099704 NPR1 3.461976 GUCY1A3 3.08975 RGS13 2.092105 PAN3-AS1 3.436176 TRAF5 3.069623 LRMP 2.089421 SMAGP 3.428144 YME1L1 3.060843 PTPRCAP 2.070706 PLEKHG4B 3.418811 CDCA7 3.057565 BCL7A 2.062017 TMED6 3.413322 ZNF423 3.035199 STAP1 2.058908 CLEC14A 3.407185 PXDN 3.032362 83

PHGDH 2.047085 TMEM263 3.40546 LZTFL1 3.030312 TLR10 2.040809 UBASH3B 3.402108 LINC01215 3.026616 BACH2 2.037776 LOC285097 3.398495 GATA3 2.996599 FAM167A 2.036025 ENAH 3.385695 BCL11B 2.995952 PDLIM1 2.014073 PCDH9 3.369264 NRXN2 2.983451 TERT 2.012773 GNAI1 3.343503 STMN3 2.97388 ITPR3 2.003871 TSPAN7 3.315523 SCGB3A1 2.973864 NTNG2 -2.00045 PIK3C2B 3.296379 MEX3B 2.96618 KAT2B -2.006 GUCY1A3 3.292083 RCBTB2 2.966055 GCLM -2.00624 AHDC1 3.287957 TSHR 2.954108 IL1R1 -2.00674 STRBP 3.274388 KCNK17 2.952778 HAGH -2.0071 BCL11A 3.255449 TASP1 2.94732 FRY -2.00755 DCHS1 3.242336 GALNT2 2.94068 FCHO2 -2.00936 ZC3H12D 3.210683 PTPN14 2.933334 IGHD -2.01074 CTHRC1 3.200537 MAL 2.932571 FAM117A -2.01097 BANK1 3.195929 CDH2 2.92161 GAS2L3 -2.0112 MSI2 3.186135 CR2 2.913723 VCAN -2.01136 IGF2BP3 3.171736 PLCB4 2.912182

Table F2. Bottom 100 DEGs with greater than - 2 log-fold-change

B-ALL with t(8;14) Pooled B-ALL without t(8;14) T-ALL Gene Log FC Gene Log FC Gene Log FC CEACAM SLC14A1 -3.2609 RGL4 -4.7311 1 -4.4257 BEX1 -3.2725 CXCL1 -4.7314 CST7 -4.4292 IGKC -3.2728 SYNE1 -4.7415 CXCL12 -4.4313 ACSL6 -3.3148 GJB6 -4.7549 GYPB -4.4360 FAXDC2 -3.3251 BST1 -4.7969 FCAR -4.4590 C17orf99 -3.3354 3/1/2017 -4.7977 NCF4 -4.4726 HP -3.3436 SELENBP1 -4.8022 GSN -4.4737 PRG2 -3.3458 CPA3 -4.8157 CD14 -4.4778 CDA -3.3907 FOLR3 -4.8215 GPR27 -4.4812 CHIT1 -3.4065 SDPR -4.8273 FOLR3 -4.4942 HBD -3.4132 ANXA1 -4.8402 RBP7 -4.4949 GAS2L1 -3.4177 TAL1 -4.8415 VSTM1 -4.5086 CD59 -3.4281 TMEM56 -4.8631 GJB6 -4.5111 SLC22A1 OSBPL1A -3.4321 VCAN -4.8668 5 -4.5112 MSRB3 -3.4412 LILRA3 -4.8754 MAP3K13 -4.5230 S100P -3.4529 S100A11 -4.9027 KCNE3 -4.5371 CYBRD1 -3.4683 SERPINA1 -4.9282 GCA -4.5377 SOX6 -3.4705 SLC22A4 -4.9339 HLXt -4.5396 KEL -3.4950 FCAR -4.9413 RHD -4.5514

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CR1L -3.4989 G0S2 -4.9462 S100A12 -4.5607 FAR2 -3.5060 SERPINB2 -4.9564 FPR1 -4.5763 CLEC11A -3.5216 OLR1 -4.9599 CYBB -4.5842 FECH -3.5535 FCER1A -4.9704 HK3 -4.5868 CA1 -3.5721 PROK2 -4.9736 OLR1 -4.5881 F13A1 -3.5732 SIRPB1 -4.9759 PADI4 -4.5952 CHI3L1 -3.5862 IL18RAP -4.9845 CHIT1 -4.6099 SLC6A8 -3.5942 CLEC7A -4.9943 P2RY13 -4.6261 PTX3 -3.5998 TREM1 -5.0154 TRIM10 -4.6433 AKR1C3 -3.6183 RETN -5.0163 KLF1 -4.6457 SLC40A1 -3.6184 PCOLCE2 -5.0489 SLC2A5 -4.6565 TACSTD2 -3.6660 SERPINB10 -5.0620 CSTA -4.6616 TRIM58 -3.6861 ECRP -5.0796 MCEMP1 -4.6624 CLC -3.6938 CLEC5A -5.1070 PCOLCE2 -4.6645 MMP9 -3.6990 MGAM -5.1713 XK -4.7074 PCOLCE2 -3.6996 FGR -5.1997 ATP8B4 -4.7166 CTSE -3.6999 CHIT1 -5.2477 STOM -4.7405 RHD -3.7437 TGFBI -5.2868 FCN1 -4.7709 SPARC -3.7855 CD36 -5.3075 TMEM56 -4.7715 LCN2 -3.8027 PRG2 -5.3272 CD24 -4.7817 TSPO2 -3.8253 SLC40A1 -5.3435 TNS1 -4.7931 CD36 -3.8297 VSTM1 -5.3602 NCF2 -4.7978 CA2 -3.8331 RBP7 -5.3647 CLEC5A -4.8134 CYP4F3 -3.8384 CLEC12A -5.3737 LYZ -4.8172 SERPINB1 0 -3.8403 QPCT -5.4146 CLEC12A -4.8214 ATP8B4 -3.8438 PTX3 -5.4153 AQP1 -4.8335 NFE2 -3.8609 FCER1G -5.4239 RNASE2 -4.8535 SLC22A4 -3.8616 P2RY13 -5.4273 SELENOP -4.8536 SERPINB XK -3.8636 FAM46A -5.4861 10 -4.8540 LTF -3.8754 EREG -5.5257 SDPR -4.8942 PADI4 -3.8772 GGTA1P -5.5290 QPCT -4.9222 FCAR -3.8904 HK3 -5.5537 AHSP -4.9273 ARG1 -3.9096 S100A12 -5.6062 ECRP -4.9315 GFI1B -3.9211 CEACAM6 -5.6271 PRG2 -4.9465 TMEM56 -3.9519 GPR27 -5.6323 RAB31 -4.9759 OLR1 -3.9697 CD14 -5.6472 CA2 -4.9774 GGTA1P -4.0080 RNASE2 -5.6961 PTX3 -4.9838 MPO -4.0227 HP -5.7107 SLC22A4 -4.9852 HBM -4.0329 FPR1 -5.7159 EPB42 -4.9866 RGS18 -4.0510 SLC22A15 -5.7385 CYP4F3 -5.0802 CAVIN2 -4.0734 TUBB1 -5.7417 CD36 -5.1075 SELENOP -4.0922 ARG1 -5.7594 ADD2 -5.1151 TAL1 -4.0975 C5AR1 -5.7833 BASP1 -5.1166 85

SLC4A1 -4.1120 RAB31 -5.8042 HP -5.1297 SELENBP ECRP -4.1271 LTF -5.8110 1 -5.1599 DEFA4 -4.1364 PRG3 -5.8149 ALAS2 -5.2075 ADD2 -4.1433 SLPI -5.8434 GGTA1P -5.2426 ABCA13 -4.1754 RAB32 -5.8462 LTF -5.2823 ELANE -4.2254 BEX1 -5.8884 MGST1 -5.2854 CXCL12 -4.2469 CSTA -5.9475 PRG3 -5.3196 SLC22A16 -4.2487 CYP4F3 -5.9767 ORM1 -5.3588 CEACAM8 -4.2543 CHI3L1 -6.0167 ARG1 -5.3726 SPTA1 -4.2553 LYZ -6.0348 MMP9 -5.3742 KLF1 -4.2684 MNDA -6.0537 CLC -5.3959 PGLYRP1 -4.2978 OLFM4 -6.0571 S100P -5.4030 ANXA3 -4.3370 TACSTD2 -6.0584 MNDA -5.4650 TCN1 -4.3426 CLC -6.1040 CHI3L1 -5.4973 RNASE3 -4.3694 MGST1 -6.1415 CDA -5.5207 AHSP -4.3728 ORM1 -6.1998 SLPI -5.5220 AQP1 -4.4365 MPO -6.2719 TACSTD2 -5.5665 CEACAM6 -4.4517 S100P -6.2756 PLBD1 -5.5717 CTSG -4.4803 MMP9 -6.3394 ANXA3 -5.5969 EPB42 -4.5984 ANXA3 -6.3622 TUBB1 -5.8225 HEMGN -4.6600 DEFA4 -6.3973 PGLYRP1 -5.8229 ALAS2 -4.6845 CDA -6.4119 CTSG -5.8598 GYPB -4.7007 LCN2 -6.4284 LCN2 -5.8770 TRIM10 -4.7142 PGLYRP1 -6.4383 OLFM4 -6.0601 RHAG -4.7280 PF4 -6.4928 DEFA4 -6.1128 AZU1 -4.7326 FCN1 -6.4978 ELANE -6.1259 MS4A3 -4.7787 CTSG -6.6003 MPO -6.2102 CEACAM ORM1 -4.7956 ELANE -6.6565 8 -6.2105 PRTN3 -4.8450 CEACAM8 -6.7162 TCN1 -6.2660 PRG3 -4.8637 TCN1 -6.7181 RNASE3 -6.3073 PPBP -4.8840 MS4A3 -6.7404 PF4 -6.3790 SELENBP1 -4.9169 CAMP -6.8136 MS4A3 -6.3977 CEACAM PF4 -4.9327 PRTN3 -6.8274 6 -6.4695 CRISP3 -4.9571 RNASE3 -6.9737 MMP8 -6.4772 GYPA -4.9707 PLBD1 -6.9815 CAMP -6.5062 MMP8 -5.0107 MMP8 -6.9987 CRISP3 -6.7073 TNS1 -5.0652 PPBP -7.0091 PRTN3 -6.7849 SNCA -5.1098 AZU1 -7.3585 AZU1 -6.7950 TUBB1 -5.4744 CRISP3 -7.4060 PPBP -6.9681 CAMP -5.4873

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Comparison of shared versus unique DEGs among 4 ALL leukemia groups

Figure F1. Comparison of DEGs among 4 subsets of Acute lymphoblastic leukemia: (includes the ALL hyperdiploid karyotype which was not included in figure 9)

ALL subset Unique DEGs B-ALL with t(8;14) 70 DEGs B-ALL with t(1;19) 410 DEGs B-ALL with t(12;21) 348 DEGs ALL with hyperdiploid karyotype 242 DEGs

In figure 10, we see great overlap between these leukemia groups as well as relatively large sets of unique genes to each subset. There are 70 DEGs unique to B-ALL with t(8;14);

410 DEGs unique to ALL with t(1;19); 348 DEGs unique to ALL with t(12;21); and 242 DEGs unique to ALL with hyperdiploid karyotype. This distribution of DEGs is further evidence of the unique phenotype of B-ALL with t(8;14).

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Table F3. Relative ranking of DEGs among 3 ALL leukemia subsets

Table F3. Relative cross-ranked table of DEGs in 3 ALL subsets

Table F3 shows a stark contrast in the relative ranking of the top DEGs between the B-ALL with t(8;14) versus the other 6 B-ALL subsets. For example, TCL1A is ranked 4th on the list of DEGs for B-ALL with t(8;14), with a 5-fold change in expression, but it is ranked

13th on the list of DEGs for pooled B-ALL, showing a 5.1-fold change. Likewise, CD19 is ranked 9th in DEGs for B-ALL with t(8;14) with a 3.8-fold change, but it is ranked 5th in DEGs for the pooled B-ALL with a 5.7-fold change. We see that for the other top 17 DEGs in the list

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for B-ALL with t(8;14), that they are located lower in rank than 20th for the top DEGs for the pooled B-ALL. This re-ordering of the top DEGs is another example of how the B-ALL with t(8;14) is distinct from the other pooled B-ALL subset.

Background on selected DEGs

TCL1 is often involved in chromosomal inversions and translocations in human T-cell leukemias and lymphomas [281]. In normal hematopoiesis, TCL1 expression is found in developing B lymphocytes [282]. Differential expression of TCL1 is seen during pre-B-cell acute lymphoblastic leukemia progression [283]. TCL1 is a putative oncogene on human chromosome 14q32.1 which becomes activated in T-cell leukemias when it is juxtaposed next to the regulatory elements of T-cell receptor genes [284]. Tcl1 protein interacts with the Akt

(protein kinase B) prosurvival pathway such that it activates Akt kinase which facilitates the translocation of Akt to the nucleus and also increases the enzymatic activity of Akt [285].

PEG10 is ranked 2nd on the list of DEGs for the B-ALL with t(8;14), showing a 4.8-fold change in expression, but lower than 20th rank for the other 6 B-ALL leukemias. Overexpression of PEG10 is associated with several malignancies, including hepatocellular carcinoma [286] and

B-cell lymphocytic leukemia [287,288].

FCRLA is the 8th ranked gene in the list of DEGs, showing a 3.8-fold change in expression, and it encodes The Fc receptor-like A protein [289]. CD19 is the 9th ranked DEG, showing a 3.8-fold change of expression, and it encodes for a cell surface protein found on B lymphocytes and follicular dendritic cells [290] CD19 assembles with the B-cell receptor for antigens. CD19 acts as a B-cell co-receptor along with CD21 and CD81. Anti-CD19 monoclonal

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antibodies and immunotoxins have been used to treat B-cell malignancies [291]. Evidence suggests that CD19 signaling downstream causes stabilization of MYC protein in lymphomagenesis [292].

RASSF6 is the 11th ranked gene on the list of DEGs for B-ALL with t(8;14), showing a

3.6-fold change in expression, but is lower than 20th ranked gene for the other B-ALL leukemias. It encodes a member of the Ras-association domain family (RASSF) [293], which is part of the tumor suppressor network Salvador-Warts-Hippo (SWH). The protein encoded by this gene is a Ras effector protein which induces apoptosis [294].

DTX1, the 12th ranked gene, showing a 3.5-fold change in expression, and it encodes the protein deltex-1 which is a positive regulator of the notch signaling pathway in D. melanogaster

[295, 296] DTX1 interacts wtih EP300 [297]. DTX1 affects Notch2 signaling in B cells and marginal zone B lineage development [298]. DTX plays a role in germinal center B cell development [299,300].

HRK, the 16th ranked gene, showing a 3.5-fold change in expression, and it encodes the protein "activator of apoptosis harakiri", which regulates apoptosis through its interaction with

Bcl-2 and Bcl-X(L) [301]. HRK has a similar pattern of cellular localization as BCL2 and

BCLXL [302]. Hrk in hematopoietic progenitor cells may be transcriptionally silenced by interleukin-3 dependent activation of DREAM [303]. Fas signaling and blockade of Bcr-Abl kinase induce apoptotic Hrk protein via DREAM inhibition in human leukemia cells [304]. ID3, the 18th ranked DEG, showing a 3.4-fold change in expression, and it plays a role in the

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induction of differentiation of erythroleukemia cells via selective antagonism of E2A basix helix- loop-helix protein [305,306].

17 DEGs of the top 20 ranked genes in the B-ALL with t(8;14) are much lower in rank in the other 6 B-ALL leukemia datasets. Running the correlation between the B-ALL with t(8;14) against the pooled B-ALLdatasets and the T-ALL dataset, both produce a correlation coefficient about r = 0.20. The low correlation between these 3 subsets of ALL further supports the unique phenotype of the B-ALL with t(8;14).

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Appendix G

KEGG pathway analysis of DEGs

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Table G1. Top biological pathways significantly impacted by the DEGs (B-ALL with t(8;14)

Pathway Name PathwayID p-value FDR p-value adj. Hematopoietic cell lineage 04640 2.483e-9 5.934e-7 5.934e-7 Porphyrin metabolism 00860 9.212e-6 0.001 0.002 Amoebiasis 05146 8.490e-5 0.007 0.020 Leishmaniasis 05140 1.440e-4 0.008 0.034 Asthma 05310 1.592e-4 0.008 0.038 Transcriptional dysreg. in cancer 05202 2.385e-4 Cell Adhesion Molecules (CAMs) 04516 3.165e-4 Nitrogen metabolism 00910 7.313e-4 Cytokine-cytokine receptor int. 04060 1.340e-3 Th17 cell differentiation 04659 2.142e-3

In table G1, the first ranked pathway "Hematopoietic cell lineage" makes logical sense because the core problem in the B-ALL t(8;14) leukemia is the arrested development of the lymphoblasts during hematopoiesis and the failure to progress forward in normal differentiation.

The second ranked pathway "porphyrin metabolism" involves the biosynthesis of the porphyrin ring which is essential to the formation of heme and the electron transport chain/oxidoreductive reactions carried forth by the cytochrome proteins in the mitochondria. There are well established disturbances in aerobic vs anaerobic metabolism ("Warburg effect") in many cancers which tend to favor anaerobic metabolism and glycolytic pathways. The glycolytic pathways also has an entry into the hexose monophosphate shunt important in the synthesis of NADPH, pentoses, ribose-5-phosphate and the nucleotides for DNA synthesis for proliferating cells.

The third (amoebiasis), fourth (Leishmaniasis) and fifth (asthma) ranked pathways all involve the adaptive immune response for which mature lymphocytes play a key role. In ALL the lymphoblasts fail to differentiate into mature lymphocytes thus crippling the normal immune response. The disruption in the immune response is also evident in the ninth (cytokine-cytokine 93

receptor interactions) and tenth (Th17 cell differentiation) biological pathways impacted by these

DEGs.

The sixth (transcriptional dysregulation in cancer), seventh (cell adhesion molecules) and eighth (nitrogen metabolism) impacted pathways also seem quite logical in terms of the cancerous nature of acute lymphoblastic leukemia. Similar to the virally-infected state, the neoplastic state hijacks the cell’s metabolism along with its nitrogen and protein synthesis machinery for its own nefarious ends resulting in transcriptional dysregulation as typically seen in neoplasia. Cell adhesion molecules play a vital role in initiating the inflammatory/immune response because of their importance in the rolling, margination and diapedesis of phagocytes, lymphocytes and other leukocytes into areas on injury/invasion. Also cell adhesion molecules also play an important role in contact inhibition of cell growth and prevention of metastasis.

Disruption of these cell adhesion molecules can facilitate neoplastic growth, invasion and metastasis.

Table G2. Comparison of KEGG biologic pathways among 3 ALL subsets

Rank B-ALL with t(8;14) Pooled B-ALL w/o t(8;14) T-ALL 1 Hematopoietic cell lineage Hematopoietic cell lineage Hematopoietic cell lineage 2 Porphyrin metabolism Phagosome Phagosome 3 Amoebiasis Lysosome Lysosome 4 Leishmaniasis Chemokine signaling pat Chemokine signaling path 5 Asthma Tuberculosis Amoebiasis 6 Transcriptional misregu cancer Amoebiasis Transcript misreg cancer 7 Cell Adhesion molecules Cell adhesion molecules Porphyrin metabolism 8 Nitrogen metabolism Transcript misrg cancer Tuberculosis 9 Cytokine-cytokine receptor int Legionellosis NF-kB signaling path 10 Th17 cell differentiation Porphyrin metabolism Osteoclast differentiation

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Table 16 shows that the KEGG pathway analysis reveals the hematopoietic cell lineage pathway to be the first ranked among all 3 ALL subsets. We see that the top 4 ranked pathways are identical for the pooled B-ALL without t(8;14) and the T-ALL subsets, but are distinct from the top 4 ranked pathways for the B-ALL with t(8;14).

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Appendix H

Gene Ontology analysis of DEGs

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Table H1. GO: Top biological processes identified in Gene Ontology analysis

Pruning Type: none

GO Term p-value p-value (FDR) p-value (Bonferroni) Immune system process 3.700e-22 2.027e-18 2.027e-18 Immune response 1.300e-15 3.561e-12 7.123e-12 Defense response 9.100e-15 1.662e-11 4.986e-11 Response to bacterium 5.200e-14 7.123e-11 2.489e-10 Response to external biotic stimulus 2.700e-11 2.466e-8 1.479e-7

Pruning Type: Elim Pruning Type: Weight

GO term p-value GO term Platelet degranulation 4.000e-9 Defense reponse to bacterium 1.900e-10 Response to lipopolysaccharide 8.800e-8 Porphyrin biosynthetic process 1.600e-9 Protoporphyrinogen IX biosyn 1.900e-6 Platelet degranulation 4.000e-9 B cell receptor signaling path 2.700e-6 Immune response 7.100e-9 Defense reponse to bacterium 4.100e-6 Defense response 3.100e-8

As expected, the Gene Ontology functional analysis of the microarray gene expression results show that the top biological processes impacted by the observed DEGs include the immune system process, immune response, defense response, response to bacterium, response to external biotic stimulus, the B cell receptor signaling pathway and the reponse to lipopolysaccharde. This makes sense because the mature lymphocytes play a key role in all these biological processes and in B-ALL with t(8;14), this is an arrest in the development and hematopoiesis of immature early progenitor lymphoblasts which greatly limit the numbers of mature lymphocytes available, and this is further limited by the overcrowding and infiltration of the bone marrow by these immature progenitor lymphoblasts.

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Figure H1 Immune system genes identified: 154 DEGs out of 2184 genes (Diagram & graphics generated by iPathway tool)

In figure H1, the 4-fold increases in the gene expression of BLK and CD19, as well as the

3-fold increase in CAMK2D, 2-fold increase of MS4A1, and significant decreases in the expression of ACTN1, ALAS2, AMPD3, ANXA1, ANXA3, AZU1, CEACAM1, BST1, CA2,

CAMP, CD33, CD36, CD58 and CD59 represent 154 DEGs observed to be modulated out of the set of 2184 immune system genes.

Figure H2. Platelet degranulation / regulated exocytosis genes identified: 18 DEGs out of 101 genes

In figure H2, we see that the signficant decreases in gene expression of ACTN1, CD36,

ITGA2B, ORM1, PF4, PPBP, QSOX1, SELENOP, SPARC, VCL, DMTN, CLU, F5 and

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PHACTR2 represent 18 DEGs observed to be modulated out of the set of 101 genes representing platelet degranulation and regulated protein exocytosis processes.

Figure H3. Bacterial defense response genes identified: 98 genes out of 1335 genes

In figure H3, we see that the observed gene expression reductions see in ANXA3, APP,

AZU1, CAMP, CD36, CEBPE, CTSG, DEFA4, ELANE, HP, IGKC, IL6R, LTF, LYZ, MPO,

PPBP, RNASE3, S100A12, SLP1 and PGLYRP1 represent 98 genes out of the set of 1335 known genes involved in the bacterial response group.

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Table H2. GO: Molecular Functions results from Gene Ontology analysis

Pruning Type: none

GO Term p-value p-value (FDR) p-value (Bonferroni) Carbohydrate binding 1.300e-8 1.397e-5 1.397e-5 Immunoglobulin receptor binding 1.500e-5 0. 008 0.016 Phenanthrene 9,10-monoxygenase 3.600e-5 0.009 0.039 Ketosteroid monoxygenase 3.600e-5 0.009 0.039 Peroxidase activity 4.200e-5 0.009 0.045 Oxido-reductase (peroxide acceptor) 7.200e-5 Glycosaminoglycan binding 1.100e-4 Trans-1,2-dihydrobenzene-1,2-diol 1.400e-4 dehyrogenase activity CXCR chemokine receptor binding 1.700e-4 Chemokine receptor binding 2.900e-4 Pruning type = Elim p- Pruning type = weight GO Term value GO Term p-value Carbohydrate binding 3.700e-6 Carbohydrate binding 1.000e-5 Immunoglobulin receptor bind. 1.500e-5 Immunoglobulin receptor bind. 1.500e-5 Phenanthrene 9,10 monoxygenase 3.600e-5 Phenanthrene 9,10 monooxygenase 3.600e-5 Ketosteroid monooxygenase 3.600e-5 Ketosteroid monooxygenase 3.600e-5 Trans-1,2-dihydrobenzene-1,2- 1.400e-4 Peroxidase activity 4.200e-5 diol dehyrogenase

In table H2, Gene Ontology analysis reveals that the top DEGs observed have impacted the molecular functions group including immunoglobulin receptor binding, carbohydrate binding and specific oxido-reductase enzymes including the ketosteroid monooxygenase, peroxidase, phenanthrene 9,10 monooxygenase and trans-1,2-dihydrobenzene-1,2-diol dehydrogenase.

Immunoglobulin receptor binding makes sense because of the role of such binding in the activation of B-cell receptors. Also the carbohydrate binding may be involved in the immune response of lymphocytes to several of the known activating lectins such as phytohemagglutinin

A (PHA) and concanavalin A (ConA). Interpretation of the effects on specific oxido-reductases

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may require a more in depth analysis of the roles of such enzymes in the immune response and lymphocyte biology.

Figure H4. GO Carbohydrate binding related genes identified: 26 genes out of 216 genes (Diagram & graphics generated by iPathway tool)

In figure H4, we see that the almost 3-fold increase in CD22, as well as the significant reductions of gene expression seen in CD33, CH13L1, CLC, GYS1, HK3, LGALX3, OLR1,

PTX3, PYGL, CLEC11A, SLC2A3, SIGLEC5, MGAM, PRG3, CLEC4D, LGALS12,

CLEC5A, LGALSL and CLEC12A represent 26 DEGs out of the set of 216 known genes in the group of carbohydrate binding genes.

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Table H3. GO: Top cellular component gene results from Gene Ontology analysis

Pruning Type: none

GO Term p-value p-value (FDR) p-value (Bonferroni) Extracellular space 2.600e-17 1.568e-14 1.568e-14 Vesicles 1.800e-15 5.427e -13 1.085e-12 Membrane-bound vesicles 3.200e-15 6.432e-13 1.930e-12 Cell periphery 7.800e-15 1.134e-12 4.703e-12 Plasma membrane 9.400e-15 1.134e-12 5.668e-12

Pruning Type: Elim Pruning Type: Weight

GO term p-value GO term p-value Extracellular exosome 1.400e-13 Extracellular vesicle 1.000e-13 Platelet alpha granule lumen 5.800e-7 Platelet alpha granule 5.600e-9 Plasma membrane 7.500e-7 Intrinsic component of membrane 2.700e-8 Specific granule 2.800e-6 Secretory granule membrane 2.000e-6

In table H3, we see that Gene Ontology analysis further reveals that the top DEGs impact specific cellular component alleles involved with the extracellular space, vesicles, membrane- bounded vesicles, the plasma membrane and cell periphery genes. When we consider the importance of specific events which happen at the cell surface of mature lymphocytes such as activation of the B-cell receptor, and secondary facilitating or inhibitory molecular interactions, it makes sense that such cell surface and extracellular genes would be significantly impacted due to the arrest of development of immature progenitor lymphoblasts in B-ALL with t(8;14).

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Figure H5. DEGs: Extracellular space / plasma membrane related genes identified: 99 genes out of 1238 genes

In figure H5, we see that the significantly impacted DEGs include the upregulated

AEBP1, BMP7 and MS4A1, as well as the downregulated ACTN1, ALAD, ANXA1, APP,

ARG1, AZU1, CA2, CAMP, CAT, CD36, CD59, CEACAM8, CHI3L1, CHIT1, CPA3 and

CSTA genes which account for 99 genes out of the group of 1238 genes involved in the extracellular space group.

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Appendix I

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Figure I1. Hematopoietic cell lineage KEGG pathway genes perturbed (Diagram & graphics generated by iPathway tool)

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Figure I2 upper part of figure KEGG analysis: DEGs in hematopoietic pathway

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Figure I3. lower half of figure I1 KEGG analysis: DEGs in Hematopoietic pathway

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Figure I4. KEGG: DEGs in hematopoietic pathway

Figure I5. KEGG: DEGs in B-ALL t(8;14)

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Cytokine-cytokine receptor interactions

Figure I6. KEGG: DEGs in cytokine-cytokine receptor pathway I

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Figure I7. KEGG: DEGs in cytokine-cytokine receptor pathway II

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Appendix J

Predicted putative modulating microRNAs

based on DEGs expressed

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Putative microRNAs modulating gene expression

Table J1. Statistical significance of the predicted miRNAs for the 3 groups: T-ALL, B-ALL with t(8;14), and pooled B-ALLs without t(8;14). These predicted miRNAs are based upon the known regulatory effects of these miRNAs and the observed modulation of the DEGs.

Figure J1. Genes modulated by microRNA hsa-miR-9-5p

In Figure J1, we see that there is 5-fold down-regulation of TNS1, with 4-fold downregulation of ADD2 and SLC4A1. There is also 3-fold down-regulation of KLF5, IGF2BP2, CDH1, DNAJA4 and EIF4E3. These modulations are consistent with the predicted effects of hsa-miR-9-5p. 112

Figure J2. Genes affected by microRNA hsa-miR-23a-3p

In Figure J2, we see depicted greater than the 4-fold decrease in expression of CXCL12, and 3-fold decrease in the expression of SOX6, CYBRD1, FAXDC2, GPAT3, SLC28A3, FAM46C, FAM46A, TMOD1, EIF4E3, FOSB, KLF3 and CPOX consistent with the known regulatory effects of hsa-miR-23a-3p.

Figure J3. Genes affected by microRNA hsa-miR-130a-5p

In figures J2, J3, J4 and J5 (J4 and J5 are below) the same genes are downregulated by different possible microRNAs predicted. Thus the microRNAs hsa-miR-130a-5p, hsa-miR-23b-3p, hsa- miR-23c, all downregulate the same genes as microRNA hsa-miR-23a-3p. Therefore the determination of which microRNA(s) are responsible for the observed downregulation must be validated experimentally.

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Figure J4. Genes affected by microRNA hsa-miR-23b-3p

Figure J5. Genes affected by microRNA hsa-miR-23c

The same genes are downregulated by the microRNAs predicted in figures J2, J3, J4 and J5. Determining which microRNA(s) may be responsible for this modulation must be validated experimentally in the laboratory.

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Table J2. Top 10 putative microRNAs impacting gene expression

miRNA name p-value FDR p-value (Bonferroni adjust.) 1. hsa-miR-9-5p 3.489e-9 1.197e-6 1.197e-6 2. hsa-miR-23a-3p 1.812e-8 1.243e-6 6.215e-6 3. hsa-miR-130a-5p 1.812e-8 1.243e-6 6.215e-6 4. hsa-miR-23b-3p 1.812e-8 1.243e-6 6.215e-6 5. hsa-miR-23c 1.812e-8 1.243e-6 6.215e-6 6. hsa-miR-124-3p 3.088e-8 1.765e-6 1.059e-5 7. hsa-miR-181a-5p 1.708e-7 4.384e-6 5.860e-5 8. hsa-miR-181c-5p 1.708e-7 4.384e-6 5.860e-5 9. hsa-miR-181b-5p 1.708e-7 4.384e-6 5.860e-5 10. hsa-miR-181d-5p 1.708e-7 4.384e-6 5.860e-5

The complete table of the putative regulatory miRNAs is found in Appendix B

The biological literature and the miRNA Database provides the relevant information on which genes' expression are under the control of regulatory microRNAs. Thus the degree of down-regulation of these genes' expression lead to the reasonable inference of the predicted presence of specific microRNAs.

Top 10 putative microRNAs affecting Gene Expression

blue = DEGs which are downregulated; red = DEGs which are upregulated

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As seen in figure above, each of the top 10 predicted microRNAs reduce the expression of approximately 40 - 45 genes, while augmenting the expression of approximately 5 - 10 genes.

These predicted miRNAs are obtained from the miRNA Database based on the known effects of these miRNAs on the regulation of //gene expression.

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Distribution of putative modulatory microRNAs among ALL groups

In figure J6 below, we see the distribution of these miRNA effects among 4 subsets of acute lymphoblastic leukemia. The greatest majority of these miRNA modulatory effects are seen in the B-ALL with t(8;14) subset where more than 90% of the total number of miRNA regulatory effects are focused.

Figure J6. Distribution of predicted microRNAs among 4 subsets of ALL

ALL subset # of Unique miRNAs affecting gene expression

B-ALL with t(8;14) 173 B-ALL with t(1;19) 3 B-ALL with t(12;21) 0 B-ALL with hyperdiploid karyotype 5

Notably, in figure J6 out of 247 putative predicted microRNAs modulating the expression of DEGs in these 4 subsets of ALL leukemia, there are 173 unique miRNAs which are affecting gene expression in the B-ALL with t(8;14) subset (70% of total number of predicted miRNAs).

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Appendix K

Relative ranks of DEGs among 3 ALL groups with adjusted p-value and log-fold change

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Appendix K Relative ranks of DEGs among: B-ALL t(8;14), pooled B-ALL, T-ALL

Relative Rank of DEGs with adjusted p-values & log fold-change

B-ALL pooled B- T- t(8;14) ALL ALL_____

Gene Adj p-value Log FC Adj p-value Log FC Adj p-val Log FC

AAED1 0.722 -0.210 0.000 -1.225 0.000 -2.871

ABCA13 0.000 -4.175 0.000 -2.847 0.000 -3.878

ABCA5 0.000 -1.345 0.000 -2.580 0.000 -0.934

ABCB10 0.000 -2.597 0.000 -2.457 0.000 -2.391

ABCB6 0.000 -2.206 0.000 -1.943 0.000 -2.287

ABCC13 0.012 -1.870 0.000 -2.047 0.000 -2.346

ABCC5 0.000 -2.115 0.000 -1.135 0.000 1.043

ABHD17B 0.569 -0.283 0.000 2.536 0.000 2.009

ABHD2 0.000 -1.324 0.000 -2.064 0.000 -1.729

ABHD5 0.000 -2.285 0.000 -3.437 0.000 -3.544

ABI2 0.026 0.337 0.000 1.646 0.000 3.163

ACKR1 0.000 -2.495 0.000 -2.885 0.000 -2.935

ACP6 0.001 -1.186 0.000 -2.242 0.000 -1.425

ACPP 0.000 -1.563 0.000 -4.467 0.000 -3.655

ACRBP 0.000 -2.102 0.000 -1.750 0.000 -1.792

ACSL1 0.006 -1.584 0.000 -3.149 0.000 -3.950

ACSL6 0.000 -3.315 0.000 -3.382 0.000 -3.176

ACTN1 0.000 -2.853 0.000 -2.419 0.000 -2.551

ACVR2B 0.000 0.868 0.000 1.241 0.000 2.647

ADA 0.850 0.101 0.000 2.844 0.000 3.966

ADAM28 0.144 -0.727 0.000 -2.542 0.000 -2.180

119

ADAM8 0.000 -1.952 0.000 -2.809 0.000 -2.913

ADAM9 0.000 -1.465 0.000 -1.512 0.000 -2.497

ADAMTS17 0.225 0.053 0.211 0.124 0.000 2.542

ADAP1 0.003 -0.825 0.000 -1.871 0.000 -2.071

ADCY9 0.606 -0.263 0.000 2.278 0.000 -0.656

ADD2 0.000 -4.143 0.000 -3.274 0.000 -5.115

ADGRA3 0.212 -0.425 0.000 2.258 0.000 1.688

ADGRE1 0.995 -0.016 0.000 -2.109 0.000 -1.544

ADGRG1 0.026 -1.070 0.000 1.245 0.000 2.359

ADGRG3 0.000 -2.401 0.000 -3.929 0.000 -3.439

ADM 0.832 -0.274 0.000 -1.559 0.000 -2.609

ADRB2 0.009 -1.136 0.000 -2.846 0.000 -3.495

AEBP1 0.000 3.284 0.000 3.827 0.000 3.674

AFDN 0.000 1.786 0.000 2.651 0.011 -0.392

AFF3 0.000 1.414 0.000 3.542 0.000 2.126

AGL 0.108 -0.561 0.000 -2.542 0.010 -0.360

AGTRAP 0.000 -2.319 0.000 -3.655 0.000 -3.791

AHDC1 0.000 1.043 0.000 3.288 0.000 1.303

AHI1 0.007 0.331 0.000 1.478 0.000 2.799

AHSP 0.000 -4.373 0.000 -4.570 0.000 -4.927

AIG1 0.000 -2.332 0.000 -4.459 0.000 -2.668

AJAP1 0.095 0.040 0.022 0.369 0.000 2.260

AK1 0.001 -1.588 0.000 0.484 0.000 -2.244

AKAP12 0.163 0.391 0.000 2.884 0.027 0.472

AKR1C1 0.000 -2.820 0.000 -3.265 0.000 -1.453

120

AKR1C2 0.000 -2.612 0.000 -2.744 0.008 -0.515

AKR1C3 0.000 -3.618 0.000 -3.219 0.492 0.228

AKTIP 0.000 -1.842 0.000 -2.682 0.000 -2.748

ALAD 0.000 -2.299 0.000 -2.857 0.000 -2.711

ALAS1 0.000 -1.616 0.000 -2.581 0.000 -2.285

ALAS2 0.000 -4.685 0.000 -4.571 0.000 -5.208

ALDH1A1 0.000 -2.242 0.000 -2.641 0.000 -3.425

ALDH2 0.000 -1.388 0.000 -4.191 0.000 -2.859

ALDH3A2 0.001 -0.873 0.000 -2.030 0.000 -3.051

ALDH3B1 0.000 -2.149 0.000 -2.932 0.000 -4.102

ALDH5A1 0.006 0.823 0.000 2.362 0.000 1.288

ALDH7A1 0.000 0.491 0.000 2.318 0.000 0.815

ALOX12 0.001 -1.496 0.000 -2.385 0.000 -2.104

ALOX5 0.043 -1.051 0.000 -1.641 0.000 -4.083

ALOX5AP 0.000 -2.054 0.000 -3.925 0.000 -3.563

AMMECR1 0.000 -2.122 0.000 -1.852 0.000 -2.330

AMN 0.000 2.752 0.003 0.364 0.000 0.885

AMPD3 0.000 -3.023 0.000 -2.602 0.000 -2.423

ANK1 0.000 -3.168 0.000 -2.942 0.000 -4.074

ANKLE1 0.003 -1.416 0.000 -1.912 0.000 -2.631

ANKRD10- IT1 0.407 0.241 0.000 2.115 0.000 1.017

ANKRD22 0.000 -2.443 0.000 -3.470 0.000 -2.930

ANKRD36B 0.141 0.834 0.000 1.433 0.000 2.229

ANKRD9 0.000 -2.587 0.000 -1.892 0.000 -2.097

ANLN 0.000 -1.501 0.000 -2.038 0.000 -1.610

121

ANO10 0.000 -2.498 0.000 -2.945 0.000 -2.669

ANPEP 0.000 -1.765 0.000 -4.278 0.000 -3.185

ANTXR1 0.190 0.144 0.007 -0.100 0.000 2.233

ANXA1 0.000 -2.447 0.000 -4.840 0.000 -2.262

ANXA3 0.000 -4.337 0.000 -6.362 0.000 -5.597

ANXA4 0.000 -2.762 0.000 -1.884 0.000 -2.770

ANXA5 0.932 -0.040 0.000 -3.065 0.000 -3.228

AOAH 0.000 -1.444 0.000 -4.133 0.000 -2.240

APCDD1 0.011 0.807 0.000 2.927 0.716 -0.039

APLP2 0.000 -0.699 0.000 -2.845 0.000 -2.570

APMAP 0.000 -1.821 0.000 -2.933 0.000 -2.569

APOBEC3B 0.000 -2.403 0.000 -3.239 0.000 -3.275

APOBR 0.000 -1.852 0.000 -3.297 0.000 -2.592

APOC1 0.000 -2.239 0.000 -3.113 0.000 -3.283

APP 0.000 -2.772 0.000 -1.666 0.000 -3.804

AQP1 0.000 -4.436 0.000 -3.155 0.000 -4.834

AQP3 0.000 -2.066 0.000 -1.137 0.755 0.064

AQP9 0.591 -0.542 0.000 -4.218 0.000 -3.168

ARAP3 0.000 -1.652 0.000 -2.028 0.000 -2.213

AREG 0.136 -1.444 0.000 -3.763 0.000 -3.198

ARG1 0.000 -3.910 0.000 -5.759 0.000 -5.373

ARHGAP18 0.000 -2.066 0.000 -1.543 0.000 -1.061

ARHGAP24 0.230 -0.602 0.000 -2.125 0.000 -3.240

ARHGAP29 0.443 0.091 0.000 2.258 0.000 2.123

ARHGAP32 0.181 -0.301 0.000 0.551 0.000 2.326

122

ARHGEF10 0.089 -0.407 0.000 2.959 0.000 2.818

ARHGEF12 0.011 -1.132 0.000 -1.543 0.000 -3.747

ARHGEF37 0.000 -2.076 0.000 -2.394 0.000 -2.405

ARHGEF40 0.001 -1.445 0.000 -1.194 0.000 -2.268

ARHGEF7 0.001 1.115 0.000 2.298 0.000 1.994

ARID5B 0.000 0.420 0.000 2.204 0.000 0.292

ARL10 0.001 1.073 0.000 2.598 0.000 1.182

ARL4A 0.010 -1.254 0.000 -2.689 0.000 -2.137

ARL4C 0.100 0.015 0.000 -2.592 0.000 1.836

ARMCX2 0.644 0.130 0.000 1.253 0.000 2.453

ARPP21 0.002 0.662 0.000 4.197 0.000 5.072

ARSD 0.000 -1.632 0.000 0.924 0.000 -2.166

ASAH1 0.002 -1.232 0.000 -3.197 0.000 -3.111

ASAP1 0.000 -1.534 0.000 -1.466 0.000 -2.318

ASAP2 0.001 -1.368 0.000 2.309 0.000 -0.887

ASGR2 0.139 -0.648 0.000 -2.431 0.000 -1.975

ASL 0.005 -0.571 0.000 -1.128 0.000 -2.594

ASMTL 0.000 0.952 0.000 2.034 0.000 1.525

ASRGL1 0.000 -1.086 0.000 -3.542 0.000 -1.348

ATAT1 0.001 0.414 0.000 1.523 0.000 2.021

ATG7 0.000 -1.487 0.000 -2.053 0.000 -2.046

ATP1B1 0.244 -0.504 0.000 -2.152 0.000 1.502

ATP2B4 0.000 -2.337 0.000 0.655 0.000 0.796

ATP2C2 0.000 -1.338 0.000 -2.035 0.000 -1.941

ATP6V0A1 0.000 -1.102 0.000 -2.449 0.000 -2.237

123

ATP6V0D1 0.000 -0.947 0.000 -1.414 0.000 -2.103

ATP6V1C1 0.003 -1.397 0.000 -1.193 0.000 -2.156

ATP8B2 0.093 0.154 0.000 2.314 0.000 1.862

ATP8B4 0.000 -3.844 0.000 -4.720 0.000 -4.717

ATPIF1 0.000 -2.321 0.000 -2.228 0.000 -1.693

ATXN1 0.001 -1.625 0.000 -2.962 0.000 -1.790

AURKA 0.001 -1.239 0.000 -2.415 0.000 -2.143

AUTS2 0.000 0.899 0.000 3.783 0.000 0.593

AZU1 0.000 -4.733 0.000 -7.359 0.000 -6.795

B3GALNT1 0.000 -1.388 0.000 -0.936 0.000 -2.137

B3GNT5 0.000 -2.815 0.000 -1.784 0.000 -2.091

B3GNT8 0.000 -1.387 0.000 -2.202 0.000 -2.000

B4GALT5 0.000 -2.144 0.000 -4.277 0.000 -4.351

B4GALT6 0.014 -0.890 0.000 2.021 0.000 2.599

BAALC 0.088 0.087 0.000 2.918 0.001 0.821

BACH2 0.000 2.038 0.000 2.527 0.248 -0.340

BAG2 0.000 0.594 0.000 -2.170 0.000 -1.358

BAG4 0.000 -1.216 0.000 -2.099 0.000 -1.639

BAHCC1 0.725 0.170 0.000 2.686 0.000 3.272

BANK1 0.406 0.286 0.000 3.196 0.000 -1.985

BASP1 0.000 -1.500 0.000 -4.689 0.000 -5.117

BCAS4 0.000 2.583 0.000 3.000 0.000 0.436

BCL11A 0.809 0.252 0.000 3.255 0.000 0.924

BCL11B 0.027 -1.538 0.000 -2.018 0.000 2.996

BCL2A1 0.076 -1.049 0.000 -4.627 0.000 -3.331

124

BCL2L15 0.000 -2.579 0.000 -2.952 0.000 -3.086

BCL6 0.107 -0.811 0.000 -2.223 0.000 -1.579

BCL7A 0.000 2.062 0.000 3.490 0.000 2.326

BCR 0.215 0.418 0.000 2.178 0.000 1.296

BEND4 0.000 2.507 0.000 0.982 0.000 1.276

BEND5 0.134 0.323 0.000 2.808 0.000 1.652

BEST3 0.000 2.969 0.000 1.466 0.036 0.382

BEX1 0.000 -3.273 0.000 -5.888 0.000 -4.357

BEX2 0.842 0.156 0.000 -3.423 0.031 -0.957

BEX3 0.011 -0.831 0.000 -4.096 0.723 0.153

BICD1 0.000 1.305 0.000 0.892 0.000 2.250

BIN2 0.000 -1.912 0.000 -3.927 0.000 -0.646

BIRC3 0.177 0.597 0.000 -2.110 0.003 -0.598

BLK 0.000 4.061 0.000 4.037 0.028 0.803

BLNK 0.001 2.375 0.000 4.901 0.000 -2.553

BLVRA 0.000 -1.924 0.000 -2.681 0.000 -2.314

BLVRB 0.000 -2.954 0.000 -3.829 0.000 -4.290

BMP2 0.000 -1.205 0.000 2.526 0.000 -1.603

BMP7 0.000 2.526 0.000 -0.263 0.000 -0.302

BMS1P20 0.000 0.960 0.000 -2.387 0.000 -2.630

BMX 0.000 -2.141 0.000 -3.028 0.000 -2.650

BORCS8 0.004 0.420 0.000 -1.713 0.000 -2.451

BPGM 0.000 -2.243 0.000 -2.562 0.000 -2.467

BRE-AS1 0.022 -1.342 0.913 -0.047 0.000 -2.014

BRI3 0.005 -0.819 0.000 -1.716 0.000 -2.097

125

BSG 0.000 -1.963 0.000 -2.463 0.000 -2.323

BST1 0.000 -3.196 0.000 -4.797 0.000 -4.379

BTK 0.112 0.560 0.000 1.006 0.000 -2.458

BTNL9 0.000 3.074 0.000 2.946 0.004 0.702

C10orf10 0.276 0.176 0.000 2.845 0.000 -0.853

C10orf11 0.000 -2.219 0.000 -3.528 0.000 -3.317

C10orf128 0.000 -1.707 0.000 -1.192 0.000 -2.104

C10orf54 0.003 -1.108 0.000 -2.576 0.000 -2.419

C11orf21 0.000 -2.415 0.000 -1.121 0.000 -1.090

C11orf74 0.000 -2.379 0.002 -0.599 0.000 -1.474

C11orf95 0.000 1.852 0.000 2.784 0.000 2.870

C12orf29 0.001 -0.915 0.000 -2.773 0.000 -1.654

C15orf48 0.753 -0.520 0.000 -2.251 0.000 -1.750

C17orf58 0.943 0.038 0.000 -2.891 0.000 -2.536

C17orf99 0.000 -3.335 0.000 -3.775 0.000 -3.595

C1orf106 0.000 -1.844 0.000 -2.060 0.000 -2.058

C1orf162 0.000 -1.613 0.000 -3.749 0.000 -4.031

C1orf228 0.000 -2.118 0.000 1.374 0.000 1.432

C1QB 0.639 -0.554 0.000 -2.412 0.000 -2.814

C1QC 0.174 -1.060 0.000 -2.673 0.000 -2.822

C1QTNF4 0.000 -0.859 0.000 2.177 0.944 -0.026

C20orf27 0.000 -1.144 0.000 -1.550 0.000 -2.053

C21orf2 0.000 0.268 0.000 2.590 0.000 0.825

C2orf88 0.000 -1.908 0.000 -3.169 0.000 -3.074

C3AR1 0.010 -1.430 0.000 -4.656 0.000 -3.710

126

C4orf32 0.075 -0.805 0.000 0.867 0.000 -2.448

C5AR1 0.001 -1.860 0.000 -5.783 0.000 -4.408

C5orf30 0.000 -1.296 0.000 -1.931 0.000 -2.264

C5orf56 0.574 0.139 0.000 3.143 0.000 2.179

C8orf88 0.037 -0.848 0.000 -2.240 0.120 0.434

CA1 0.000 -3.572 0.000 -4.164 0.000 -4.149

CA2 0.000 -3.833 0.000 -3.984 0.000 -4.977

CA3 0.000 -2.574 0.000 -3.101 0.000 -2.944

CA4 0.002 -2.071 0.000 -4.039 0.000 -2.656

CACNA2D3 0.000 -2.100 0.000 -2.387 0.000 -2.256

CACNB3 0.053 0.109 0.000 3.161 0.000 3.348

CADM1 0.000 2.364 0.147 -0.086 0.027 0.257

CALD1 0.000 -2.038 0.028 0.204 0.000 -2.235

CALN1 0.440 0.035 0.000 2.409 0.000 1.263

CAMK2D 0.000 2.787 0.000 1.525 0.210 0.060

CAMK4 0.079 0.788 0.037 -0.487 0.000 3.350

CAMP 0.000 -5.487 0.000 -6.814 0.000 -6.506

CAPG 0.000 -2.203 0.000 -1.679 0.001 -0.697

CAPN2 0.017 -1.128 0.000 -2.086 0.000 -2.122

CAPN3 0.000 -2.305 0.000 -1.040 0.000 -3.230

CARD16 0.088 -0.791 0.000 -2.583 0.000 -1.900

CARD6 0.010 -1.363 0.000 -1.918 0.000 -2.073

CARMIL2 0.000 1.979 0.000 1.366 0.000 2.314

CASC15 0.650 -0.027 0.000 1.550 0.000 4.331

CAST 0.000 -1.251 0.000 -1.934 0.000 -3.058

127

CAT 0.000 -2.094 0.000 -1.756 0.000 -2.872

CAVIN2 0.000 -4.073 0.000 -4.827 0.000 -4.894

CBFA2T3 0.108 0.678 0.000 2.781 0.000 2.592

CBX2 0.000 1.095 0.000 1.844 0.000 2.333

CCDC125 0.000 -1.780 0.000 -2.418 0.000 -2.148

CCDC126 0.001 -1.048 0.000 -1.952 0.000 -2.080

CCDC136 0.012 0.346 0.000 0.922 0.000 2.852

CCDC50 0.000 1.165 0.000 2.284 0.000 -2.907

CCL4 0.806 0.245 0.000 -2.629 0.000 -2.578

CCL5 0.319 -0.666 0.000 -2.912 0.000 -3.459

CCNA2 0.127 -0.453 0.000 -2.128 0.000 -1.738

CCNB1 0.056 -0.760 0.000 -2.072 0.000 -1.839

CCND2 0.000 -2.316 0.000 0.846 0.000 -2.064

CCNDBP1 0.000 -1.284 0.000 -1.446 0.000 -2.133

CCNE1 0.007 -1.036 0.000 -2.091 0.000 -1.942

CCPG1 0.000 -1.765 0.000 -2.676 0.000 -2.990

CCR1 0.027 -1.050 0.000 -3.856 0.000 -3.935

CCR2 0.023 -2.019 0.000 -2.640 0.000 -2.439

CCR7 0.358 -0.817 0.000 -1.282 0.000 -2.023

CCR9 0.017 0.087 0.685 0.004 0.000 2.480

CD101 0.001 -1.261 0.000 -2.304 0.000 -2.137

CD109 0.522 -0.035 0.000 3.115 0.000 2.632

CD14 0.033 -1.484 0.000 -5.647 0.000 -4.478

CD163 0.007 -1.888 0.000 -3.808 0.000 -3.385

CD177 0.003 -2.597 0.000 -4.720 0.000 -4.139

128

CD19 0.000 3.783 0.000 5.705 0.000 -1.314

CD1A 0.056 0.096 0.011 0.194 0.000 3.458

CD1B 0.844 -0.026 0.860 -0.009 0.000 4.639

CD1D 0.776 0.253 0.000 -3.795 0.000 -1.283

CD1E 0.463 -0.055 0.556 0.033 0.000 4.892

CD2 0.489 -0.627 0.000 -2.563 0.001 1.329

CD200 0.196 -0.458 0.000 3.809 0.000 1.879

CD22 0.000 2.535 0.000 3.776 0.000 -0.624

CD24 0.000 -1.285 0.000 -0.976 0.000 -4.782

CD247 0.245 -0.809 0.000 -2.055 0.001 0.778

CD300A 0.000 -1.173 0.000 -2.097 0.000 -1.172

CD300LF 0.005 -1.185 0.000 -3.418 0.000 -3.491

CD33 0.000 -2.373 0.000 -3.678 0.000 -3.711

CD34 0.839 0.016 0.000 3.482 0.000 1.073

CD36 0.000 -3.830 0.000 -5.307 0.000 -5.107

CD3D 1.000 0.005 0.000 -2.027 0.000 4.015

CD3E 0.497 -0.418 0.000 -1.611 0.000 2.899

CD3G 0.496 0.536 0.000 -1.571 0.000 3.926

CD55 0.142 -0.596 0.000 -1.764 0.000 -2.270

CD58 0.000 -2.144 0.002 -0.564 0.000 -2.326

CD59 0.000 -3.428 0.000 -3.676 0.000 -4.094

CD63 0.000 -1.114 0.000 -2.217 0.000 -2.311

CD7 0.411 0.312 0.000 -0.792 0.000 3.240

CD72 0.000 2.780 0.000 3.928 0.000 -0.986

CD74 0.000 1.202 0.000 1.525 0.000 -2.427

129

CD79A 0.000 3.143 0.000 3.729 0.351 -0.224

CD79B 0.000 3.425 0.000 3.708 0.000 -0.856

CD81 0.000 1.510 0.000 2.012 0.000 1.155

CD86 0.097 0.621 0.000 -2.557 0.000 -3.263

CD8A 0.890 -0.131 0.000 -2.579 0.914 -0.048

CD9 0.682 -0.294 0.000 2.959 0.000 -2.897

CD96 0.021 -0.841 0.026 0.483 0.000 2.735

CD99 0.112 -0.565 0.000 1.634 0.000 2.734

CDA 0.000 -3.391 0.000 -6.412 0.000 -5.521

CDC14B 0.000 -1.827 0.000 -3.204 0.000 -3.273

CDC42BPA 0.000 -2.400 0.000 -2.729 0.000 -2.309

CDCA7 0.000 1.066 0.000 1.383 0.000 3.058

CDH1 0.000 -3.220 0.000 -3.952 0.000 -3.745

CDH2 0.567 -0.061 0.016 0.449 0.000 2.922

CDK1 0.001 -1.298 0.000 -2.178 0.000 -1.621

CDK14 0.012 0.445 0.000 0.846 0.000 -2.184

CDK6 0.000 -1.560 0.000 2.361 0.000 3.842

CDKN2A 0.000 1.364 0.000 2.024 0.001 0.472

CDKN2AIPN L 0.145 0.391 0.000 0.767 0.000 2.189

CDKN3 0.028 -0.913 0.000 -2.470 0.000 -2.002

CEACAM1 0.000 -3.198 0.000 -4.648 0.000 -4.426

CEACAM21 0.000 -2.402 0.000 -2.170 0.000 -3.038

CEACAM3 0.000 -1.672 0.000 -3.186 0.000 -3.140

CEACAM6 0.000 -4.452 0.000 -5.627 0.000 -6.470

CEACAM8 0.000 -4.254 0.000 -6.716 0.000 -6.211

130

CEBPA 0.000 -2.082 0.000 -2.705 0.000 -3.574

CEBPB 0.033 -0.731 0.000 -3.166 0.000 -2.113

CEBPD 0.000 -1.890 0.000 -4.320 0.000 -3.733

CEBPE 0.000 -2.798 0.000 -3.331 0.000 -3.429

CECR1 0.000 -2.775 0.000 -1.047

CEL 0.000 0.777 0.000 2.084 0.000 2.636

CEP128 0.045 0.209 0.000 0.600 0.000 2.537

CEP68 0.006 0.261 0.000 2.725 0.000 1.687

CEP70 0.002 0.970 0.000 1.872 0.000 3.566

CEP85L 0.030 0.222 0.000 -0.908 0.000 2.424

CES1 0.000 -2.376 0.000 -4.287 0.000 -3.771

CFD 0.028 -1.010 0.000 -4.545 0.000 -3.517

CFLAR 0.000 -1.467 0.000 -2.144 0.000 -1.598

CFP 0.000 -1.543 0.000 -4.076 0.000 -3.506

CHAC2 0.319 -0.664 0.000 -2.033 0.000 -1.967

CHD7 0.037 0.633 0.000 0.849 0.000 -2.986

CHI3L1 0.000 -3.586 0.000 -6.017 0.000 -5.497

CHI3L2 0.000 1.572 0.366 0.178 0.000 5.382

CHIT1 0.000 -3.406 0.000 -5.248 0.000 -4.610

CHML 0.000 0.640 0.000 2.071 0.000 1.428

CHPT1 0.000 -1.501 0.000 -1.171 0.000 -2.130

CHRNA3 0.626 -0.026 0.039 0.227 0.000 3.180

CHST15 0.002 0.724 0.000 2.346 0.000 -2.480

CIB2 0.002 0.582 0.056 0.090 0.000 2.168

CIDEB 0.012 -0.761 0.000 -1.261 0.000 -2.807

131

CIITA 0.004 0.042 0.000 3.617 0.000 -0.894

CITED2 0.000 -2.303 0.000 -2.159 0.000 -2.329

CKAP4 0.000 -1.862 0.000 -4.088 0.000 -3.713

CLC 0.000 -3.694 0.000 -6.104 0.000 -5.396

CLCN4 0.018 -0.944 0.000 -1.962 0.000 -2.403

CLEC11A 0.000 -3.522 0.002 -0.757 0.000 -1.057

CLEC12A 0.000 -2.943 0.000 -5.374 0.000 -4.821

CLEC14A 0.841 -0.048 0.000 3.407 0.509 -0.057

CLEC1B 0.000 -1.900 0.000 -2.656 0.000 -2.528

CLEC4A 0.000 -2.127 0.000 -2.640 0.000 -2.672

CLEC4D 0.000 -2.641 0.000 -4.704 0.000 -4.116

CLEC4E 0.001 -1.972 0.000 -2.709 0.000 -3.593

CLEC5A 0.000 -3.175 0.000 -5.107 0.000 -4.813

CLEC7A 0.254 -0.706 0.000 -4.994 0.000 -3.345

CLECL1 0.000 3.107 0.000 1.534 0.168 0.109

CLIC2 0.000 -2.287 0.000 -2.597 0.000 -2.862

CLIP2 0.996 0.005 0.000 3.950 0.000 1.522

CLIP3 0.045 0.089 0.002 0.373 0.000 3.281

CLN8 0.062 -0.637 0.000 2.071 0.000 -1.011

CLTCL1 0.000 -3.154 0.000 -3.293 0.000 -3.491

CLU 0.000 -2.148 0.000 -4.240 0.000 -4.135

CMAHP 0.000 -1.168 0.295 0.020 0.000 2.519

CMBL 0.000 -2.325 0.000 0.956 0.000 1.056

CMTM2 0.000 -2.314 0.075 -0.576 0.000 -1.406

CMTM7 0.602 -0.249 0.000 2.064 0.000 -1.234

132

CMTM8 0.993 0.011 0.000 4.191 0.005 0.691

CNIH4 0.000 -1.084 0.000 -2.172 0.000 -2.034

CNN2 0.000 -1.082 0.000 -2.100 0.000 -2.156

CNR1 0.000 3.482 0.028 0.543 0.005 -0.103

CNRIP1 0.000 -2.863 0.000 -4.667 0.000 -3.814

COL17A1 0.000 -2.975 0.000 -4.149 0.000 -3.906

COL1A1 0.041 0.201 0.000 -0.537 0.000 -2.096

COL27A1 0.003 0.307 0.000 2.511 0.085 0.209

COL5A1 0.071 0.419 0.000 4.760 0.000 1.794

COQ2 0.000 -0.819 0.000 -1.427 0.000 -2.132

CORO1C 0.000 -0.876 0.000 -2.485 0.000 -1.505

CORO2A 0.000 -1.503 0.000 -2.583 0.000 -1.385

COTL1 0.180 0.897 0.000 -3.273 0.000 -2.445

CPA3 0.000 -2.771 0.000 -4.816 0.000 -3.513

CPD 0.000 -1.530 0.000 -2.621 0.000 -2.432

CPEB2 0.000 -1.325 0.000 -2.082 0.000 -2.110

CPEB4 0.000 -2.089 0.000 -2.241 0.000 -3.023

CPM 0.072 -0.755 0.000 -0.091 0.000 -2.169

CPNE2 0.010 -1.069 0.046 -0.346 0.000 -2.825

CPNE3 0.000 -2.645 0.000 -2.154 0.000 -2.787

CPOX 0.000 -2.651 0.000 -3.057 0.000 -2.702

CPPED1 0.011 -1.109 0.000 -2.680 0.000 -2.343

CPVL 0.000 -1.683 0.000 -4.415 0.000 -2.462

CR1 0.021 -0.881 0.000 -2.726 0.000 -1.340

CR1L 0.000 -3.499 0.000 -3.858 0.000 -3.949

133

CR2 0.146 0.037 0.048 0.321 0.000 2.914

CRAMP1 0.024 0.630 0.000 2.126 0.000 0.900

CREB3L4 0.636 0.238 0.000 2.008 0.000 1.402

CREBL2 0.000 -1.368 0.000 -1.616 0.000 -2.162

CREG1 0.000 -1.105 0.000 -1.597 0.000 -2.180

CRIM1 0.037 0.495 0.000 3.942 0.003 0.636

CRISP2 0.000 -3.079 0.000 -3.861 0.000 -3.506

CRISP3 0.000 -4.957 0.000 -7.406 0.000 -6.707

CRISPLD2 0.000 -2.302 0.000 -4.455 0.000 -3.672

CRLS1 0.000 -1.049 0.000 -2.109 0.000 -1.636

CRNDE 0.008 1.235 0.000 1.837 0.000 4.334

CRTAP 0.000 -1.826 0.000 0.530 0.000 -2.406

CSF2RA 0.000 -1.812 0.000 -3.001 0.000 -2.449

CSF2RB 0.001 -1.322 0.000 -2.635 0.000 -4.138

CSF3R 0.000 -2.181 0.000 -3.379 0.000 -3.285

CSGALNACT 1 0.000 -2.372 0.001 -0.763 0.000 -2.501

CSNK1E 0.000 1.676 0.000 2.432 0.000 1.763

CSRNP2 0.011 0.182 0.000 1.387 0.000 2.334

CSRP2 0.000 1.562 0.000 6.736 0.002 0.830

CST3 0.002 -0.459 0.000 -4.181 0.000 -3.402

CST7 0.000 -2.665 0.000 -4.653 0.000 -4.429

CSTA 0.000 -2.324 0.000 -5.947 0.000 -4.662

CTBP2 0.000 -1.820 0.000 0.688 0.000 -2.404

CTDSPL 0.000 -2.866 0.000 -1.852 0.000 -0.888

CTGF 0.300 -0.495 0.000 5.233 0.000 -0.584

134

CTHRC1 0.365 -0.402 0.000 3.201 0.000 4.930

CTNNA1 0.002 -1.002 0.002 -0.507 0.000 -3.120

CTSA 0.000 -1.211 0.000 -2.320 0.000 -2.254

CTSB 0.001 -1.512 0.000 -1.955 0.000 -2.800

CTSC 0.101 -0.740 0.000 -3.871 0.000 -1.654

CTSD 0.000 -1.689 0.000 -3.519 0.000 -2.354

CTSE 0.000 -3.700 0.000 -3.750 0.000 -3.746

CTSG 0.000 -4.480 0.000 -6.600 0.000 -5.860

CTSH 0.002 -1.238 0.000 -4.051 0.000 -2.822

CTSL 0.122 -0.970 0.000 -3.115 0.000 -3.188

CTSS 0.085 -0.720 0.000 -2.563 0.000 -3.043

CTSW 0.008 -1.328 0.000 -2.752 0.000 -1.218

CTSZ 0.120 0.129 0.000 -2.334 0.000 -2.806

CTTN 0.000 -2.435 0.000 -3.081 0.000 -3.055

CX3CR1 0.135 0.201 0.000 -1.836 0.000 -2.022

CXADR 0.281 -0.474 0.000 -2.176 0.010 0.168

CXCL1 0.000 -2.109 0.000 -4.731 0.000 -3.706

CXCL12 0.000 -4.247 0.000 -3.214 0.000 -4.431

CXCL16 0.999 -0.019 0.000 -2.610 0.000 -2.875

CXCL2 0.642 0.242 0.000 -3.862 0.000 -1.967

CXCL3 0.939 0.118 0.000 -2.729 0.187 -0.420

CXCL8 0.192 -1.562 0.000 -4.215 0.000 -2.761

CXCR2 0.010 -1.782 0.000 -3.625 0.000 -3.257

CYB5R1 0.000 -1.086 0.000 -1.008 0.000 -2.011

CYBA 0.012 -0.874 0.000 -1.257 0.000 -2.285

135

CYBB 0.000 -1.923 0.000 -4.085 0.000 -4.584

CYBRD1 0.000 -3.468 0.000 -3.571 0.000 -3.430

CYGB 0.770 -0.071 0.000 4.276 0.341 -0.092

CYP1B1 0.005 -1.448 0.000 -3.784 0.000 -3.444

CYP4F2 0.000 -1.445 0.000 -2.653 0.000 -0.811

CYP4F3 0.000 -3.838 0.000 -5.977 0.000 -5.080

CYSTM1 0.000 -2.047 0.000 -4.241 0.000 -3.500

CYTH2 0.000 1.286 0.000 1.597 0.000 2.878

CYTIP 0.364 0.377 0.008 -0.584 0.000 -2.662

CYTL1 0.000 -2.216 0.017 0.986 0.000 -2.522

DACH1 0.000 -2.399 0.000 -3.034 0.000 -2.349

DAPP1 0.255 0.370 0.000 -1.458 0.000 -2.296

DBN1 0.000 1.791 0.000 3.610 0.000 3.662

DCAF12 0.000 -2.082 0.000 -1.931 0.000 -2.337

DCHS1 0.052 0.185 0.000 3.242 0.000 2.704

DCLRE1A 0.000 -1.339 0.000 -2.292 0.000 -1.469

DDAH2 0.060 -0.621 0.000 2.466 0.000 1.981

DDIT4L 0.846 -0.045 0.000 2.981 0.000 0.785

DEF8 0.000 1.210 0.000 -1.547 0.000 -2.049

DEFA4 0.000 -4.136 0.000 -6.397 0.000 -6.113

DEFB1 0.001 -2.550 0.000 -2.662 0.000 -2.252

DEGS1 0.000 -1.533 0.000 -1.866 0.000 -2.525

DENND1A 0.002 -0.562 0.000 -0.996 0.000 -2.126

DGKE 0.000 0.696 0.000 2.083 0.000 2.644

DGKH 0.058 0.184 0.000 2.001 0.000 0.730

136

DHCR7 0.002 -1.399 0.000 -2.757 0.000 -1.518

DHRS13 0.000 -1.885 0.000 -2.270 0.000 -1.995

DHRS7 0.000 -1.154 0.000 -2.538 0.000 -1.132

DHRS9 0.000 -2.337 0.000 -3.230 0.000 -3.448

DHX30 0.000 0.891 0.000 0.818 0.000 2.157

DIAPH2 0.000 -1.464 0.000 -2.055 0.000 -1.567

DIP2C 0.000 1.537 0.000 2.453 0.003 -0.023

DIRC2 0.016 -0.998 0.000 -2.385 0.000 -1.921

DLC1 0.000 -1.643 0.000 -1.669 0.000 -2.013

DLG5 0.633 -0.162 0.000 1.423 0.000 2.026

DLGAP5 0.000 -1.535 0.000 -2.370 0.000 -2.165

DLL1 0.029 0.706 0.000 2.811 0.106 0.363

DMD 0.000 2.396 0.025 0.433 0.000 1.155

DMTN 0.000 -2.876 0.000 -3.056 0.000 -3.335

DMXL2 0.014 -1.049 0.000 -2.092 0.000 -0.866

DNAH10 0.000 -2.215 0.000 -1.732 0.000 -1.495

DNAJA4 0.000 -3.104 0.000 -2.874 0.000 -2.626

DNAJC6 0.000 -2.171 0.000 -3.561 0.000 -2.949

DNMT3A 0.001 0.375 0.000 2.028 0.000 2.222

DNMT3B 0.887 0.057 0.000 1.002 0.000 2.063

DNTT 0.000 -2.845 0.000 5.610 0.000 4.540

DOCK5 0.000 -1.318 0.000 -4.501 0.000 -1.870

DPEP1 0.919 0.013 0.000 4.063 0.000 1.768

DPEP2 0.055 -0.911 0.000 -2.147 0.000 -1.622

DPPA4 0.000 -1.333 0.000 -2.635 0.000 -2.070

137

DPY19L1P1 0.000 -2.609 0.000 -2.796 0.000 -2.648

DRAM1 0.000 -1.813 0.679 0.102 0.000 -2.699

DSE 0.001 -1.788 0.000 -0.746 0.000 -2.324

DSG2 0.011 0.377 0.003 0.843 0.000 2.086

DTD1 0.003 0.599 0.000 -3.371 0.000 -2.379

DTX1 0.000 3.542 0.000 1.483 0.001 0.815

DTX3 0.000 1.617 0.127 -0.077 0.000 2.628

DUSP6 0.000 -2.199 0.045 -0.401 0.000 -2.182

DUXAP10 0.026 0.436 0.000 1.090 0.000 2.225

DYRK2 0.000 1.063 0.000 2.468 0.000 2.000

DYSF 0.000 -2.185 0.000 -3.837 0.000 -3.769

DZIP1L 0.000 -1.525 0.000 -2.203 0.000 -1.906

E2F5 0.000 2.586 0.000 0.934 0.000 0.544

EBF1 0.000 2.443 0.000 5.649 0.000 -1.567

ECM1 0.379 0.120 0.000 5.376 0.001 0.389

ECRP 0.000 -4.127 0.000 -5.080 0.000 -4.932

EFHD2 0.024 -0.695 0.000 -1.969 0.000 -2.098

EFNA1 0.338 0.105 0.000 2.195 0.000 1.246

EFNB2 0.546 -0.176 0.000 2.124 0.206 0.234

EGFL7 0.367 -0.287 0.000 2.424 0.001 -0.575

EGLN1 0.210 -0.514 0.089 -0.048 0.000 -2.454

EGR1 0.004 -1.412 0.000 -1.625 0.000 -2.247

EHD1 0.591 -0.217 0.000 -2.313 0.000 -1.525

EHD4 0.398 -0.209 0.000 -1.188 0.000 -2.844

EIF3F 0.024 0.786 0.000 2.058 0.000 1.721

138

EIF3M 0.000 0.646 0.000 0.856 0.000 2.175

EIF4E3 0.000 -2.879 0.000 -3.331 0.000 -2.452

ELANE 0.000 -4.225 0.000 -6.656 0.000 -6.126

ELFN2 0.006 0.139 0.000 2.662 0.000 0.614

ELL2 0.000 -2.176 0.000 -1.916 0.000 -3.918

ELL3 0.000 2.512 0.000 1.899 0.106 0.189

ELOVL4 0.136 0.185 0.550 -0.033 0.000 4.991

ELP2 0.001 0.715 0.000 1.932 0.000 2.261

EMB 0.000 -2.060 0.000 -1.613 0.000 -1.930

EML6 0.000 3.213 0.288 -0.103 0.000 0.779

ENAH 0.000 1.721 0.000 3.386 0.000 0.957

ENG 0.000 -1.732 0.000 2.238 0.000 -1.353

ENPP4 0.000 -1.738 0.000 -3.616 0.000 -2.041

ENTPD1 0.000 -2.344 0.000 -3.106 0.000 -3.542

EPB42 0.000 -4.598 0.000 -4.617 0.000 -4.987

EPCAM 0.000 -3.199 0.000 -4.373 0.000 -2.816

EPDR1 0.000 -2.248 0.004 -0.781 0.073 -0.548

EPHB6 0.396 -0.180 0.047 0.238 0.000 3.213

EPOR 0.000 -2.773 0.000 -3.162 0.000 -3.212

EPX 0.000 -2.804 0.000 -4.366 0.000 -4.024

EREG 0.008 -1.880 0.000 -5.526 0.000 -4.167

ERG 0.000 -2.266 0.000 2.965 0.000 1.039

ERGIC1 0.000 0.855 0.000 2.318 0.000 1.231

ERLIN1 0.000 -2.017 0.000 -3.045 0.000 -2.815

ERMAP 0.000 -2.422 0.000 -2.630 0.000 -2.490

139

ERN1 0.011 -1.193 0.000 -2.189 0.012 -0.101

ERO1B 0.481 0.295 0.000 2.415 0.001 0.559

ERVH-3 0.702 0.015 0.000 2.872 0.581 -0.007

ETS2 0.507 0.141 0.000 2.066 0.000 0.467

ETV5 0.003 0.216 0.000 2.443 0.000 1.564

EVI5 0.175 0.292 0.001 -0.373 0.000 -2.010

EVL 0.197 0.629 0.000 1.052 0.000 2.512

F13A1 0.000 -3.573 0.000 -1.175 0.000 -3.950

F5 0.000 -2.095 0.000 -4.072 0.000 -3.324

FABP4 0.001 -1.870 0.000 -2.497 0.000 -2.622

FADS3 0.000 2.243 0.000 3.601 0.001 0.622

FAH 0.000 -1.562 0.000 -2.144 0.000 -0.632

FAIM 0.001 1.605 0.000 2.086 0.000 1.812

FAM105A 0.000 -2.396 0.000 -2.994 0.000 -2.361

FAM107B 0.000 -1.009 0.000 -0.789 0.000 -2.836

FAM110C 0.411 0.014 0.000 0.916 0.000 3.169

FAM117A 0.000 -2.011 0.000 -1.713 0.000 -0.883

FAM127A 0.000 -2.204 0.000 -2.477

FAM129A 0.009 -1.271 0.000 -2.249 0.000 -1.432

FAM129C 0.000 2.201 0.000 3.671 0.000 -0.834

FAM13A 0.000 -2.696 0.000 -2.264 0.000 -0.458

FAM150B 0.000 3.470 0.030 -0.075

FAM167A 0.000 2.036 0.000 0.570 0.084 -0.060

FAM169A 0.842 0.060 0.001 0.615 0.000 2.082

FAM171A1 0.076 0.434 0.000 2.066 0.000 1.174

140

FAM198B 0.030 -1.452 0.000 -4.122 0.000 -3.147

FAM201A 0.000 -1.613 0.000 -2.183 0.000 -2.109

FAM210B 0.000 -2.778 0.000 -2.987 0.000 -3.259

FAM213A 0.001 0.410 0.000 2.719 0.000 1.238

FAM214B 0.000 -1.444 0.000 -1.790 0.000 -2.117

FAM46A 0.000 -3.011 0.000 -5.486 0.000 -3.798

FAM46C 0.000 -3.049 0.000 -3.504 0.000 -3.392

FAM49A 0.011 0.608 0.000 1.762 0.000 -2.251

FAM65C 0.000 -3.813 0.000 -4.032

FAM69B 0.462 -0.051 0.000 3.789 0.000 -0.779

FAM69C 0.014 0.082 0.000 2.611 0.303 0.091

FAM83D 0.000 -1.461 0.000 -2.301 0.000 -1.530

FAR2 0.000 -3.506 0.000 -2.864 0.000 -3.124

FAS 0.000 -1.638 0.000 -3.111 0.000 -2.712

FAT1 0.216 0.273 0.000 1.131 0.000 3.997

FAXDC2 0.000 -3.325 0.000 -3.428 0.000 -3.617

FBN1 0.000 -2.070 0.000 -3.149 0.000 -1.624

FBN2 0.000 -2.730 0.000 -3.161 0.000 -3.629

FBP1 0.744 -0.243 0.000 -3.895 0.000 -3.352

FBRSL1 0.000 1.120 0.000 2.087 0.000 1.563

FBXO41 0.000 1.188 0.000 2.290 0.000 2.479

FBXO6 0.112 -0.697 0.000 -1.273 0.000 -2.547

FCAR 0.000 -3.890 0.000 -4.941 0.000 -4.459

FCER1A 0.000 -2.828 0.000 -4.970 0.000 -3.238

FCER1G 0.001 -1.624 0.000 -5.424 0.000 -4.224

141

FCGR1B 0.010 -1.318 0.000 -4.183 0.000 -4.009

FCGR2A 0.258 -0.571 0.000 -3.333 0.000 -3.478

FCGR2C 0.046 -0.387 0.000 -2.422 0.000 -2.406

FCGR3B 0.479 -0.685 0.000 -3.918 0.000 -3.368

FCGRT 0.000 -1.574 0.000 -1.577 0.000 -2.222

FCHO2 0.000 -2.009 0.000 -1.751 0.000 -3.938

FCN1 0.000 -1.920 0.000 -6.498 0.000 -4.771

FCRL1 0.185 0.880 0.000 -1.555 0.000 -2.291

FCRL2 0.000 2.753 0.022 -0.291 0.000 -0.431

FCRLA 0.000 3.796 0.000 -0.931 0.000 -1.666

FECH 0.000 -3.554 0.000 -3.423 0.000 -2.904

FERMT2 0.265 0.078 0.000 2.057 0.067 0.366

FES 0.000 -2.213 0.000 -1.290 0.000 -2.913

FGD2 0.039 0.101 0.000 -0.871 0.000 -2.680

FGD4 0.006 -1.275 0.000 -2.730 0.000 -2.719

FGF13 0.000 -2.959 0.000 -3.359 0.000 -3.541

FGFBP2 0.813 -0.285 0.000 -2.227 0.000 -2.256

FGFR1 0.000 -0.971 0.000 0.956 0.000 2.693

FGFR1OP2 0.000 -1.274 0.000 -2.259 0.000 -2.084

FGL2 0.206 -1.171 0.000 -4.612 0.000 -3.438

FGR 0.002 -1.340 0.000 -5.200 0.000 -3.569

FHDC1 0.000 -2.745 0.000 -2.922 0.000 -2.800

FHIT 0.473 0.278 0.000 2.191 0.002 0.882

FHL1 0.001 -1.372 0.000 3.114 0.000 0.771

FHL2 0.000 -2.456 0.000 -3.651 0.000 -2.964

142

FKBP1B 0.055 -0.893 0.000 -2.602 0.001 -0.971

FLCN 0.000 -1.258 0.000 -1.218 0.000 -2.076

FLJ10038 0.001 1.001 0.000 2.203 0.000 2.109

FLNB 0.104 0.342 0.000 2.324 0.000 0.887

FLOT2 0.000 -1.063 0.000 -1.938 0.000 -2.335

FLT3 0.112 -0.639 0.000 3.699 0.793 0.104

FLT4 0.708 0.020 0.000 2.335 0.000 0.236

FMNL2 0.000 1.298 0.000 3.097 0.138 -0.226

FNDC3B 0.000 -1.873 0.000 -3.887 0.000 -3.415

FOLR3 0.000 -2.486 0.000 -4.821 0.000 -4.494

FOS 0.000 -2.075 0.000 -1.131 0.000 -2.404

FOSB 0.000 -2.841 0.000 -1.442 0.000 -1.974

FOSL2 0.002 -1.681 0.000 -1.423 0.000 -2.613

FOXO1 0.000 1.119 0.000 2.072 0.000 -2.425

FPGS 0.001 0.732 0.000 2.260 0.000 1.008

FPR1 0.000 -2.078 0.000 -5.716 0.000 -4.576

FPR2 0.001 -1.931 0.000 -3.346 0.000 -3.106

FRAT2 0.000 -1.907 0.000 -2.855 0.000 -2.394

FRMD3 0.000 -1.803 0.000 -2.535 0.000 -2.671

FRMD4B 0.000 -2.149 0.000 1.695 0.059 0.055

FRY 0.000 -2.008 0.000 -3.502 0.000 -2.613

FUCA2 0.000 -1.461 0.000 -4.162 0.000 -3.154

FUT4 0.000 -2.225 0.000 -2.192 0.000 -2.176

FXN 0.036 -0.626 0.000 -1.751 0.000 -2.686

FXYD2 0.000 0.757 0.003 0.354 0.000 3.606

143

FYB 0.000 -2.055 0.000 -3.135 0.000 -1.868

FZD5 0.000 -2.240 0.000 -2.733 0.000 -2.670

FZD6 0.001 -1.107 0.810 0.098 0.000 2.234

G0S2 0.104 -1.220 0.000 -4.946 0.000 -3.530

G6PD 0.000 -1.524 0.000 -2.005 0.000 -2.072

GAB1 0.020 -0.931 0.000 2.977 0.000 -1.888

GABARAPL1 0.095 -0.987 0.000 -2.492 0.000 -2.313

GABPB1-AS1 0.003 1.436 0.000 2.736 0.000 2.581

GALC 0.000 -1.150 0.000 -2.325 0.000 -2.386

GALNS 0.000 -1.895 0.000 -2.217 0.000 -2.434

GALNT11 0.000 -1.297 0.000 -3.006 0.000 -2.654

GALNT2 0.335 -0.285 0.000 2.289 0.000 2.941

GALNT3 0.000 -1.835 0.000 -2.058 0.000 -1.865

GALNT6 0.001 -1.309 0.000 -0.887 0.000 2.464

GALNT7 0.559 -0.323 0.000 2.309 0.000 1.615

GAPT 0.001 -1.780 0.000 -1.991 0.000 -2.710

GAS2 0.730 -0.022 0.640 0.009 0.000 2.026

GAS2L1 0.000 -3.418 0.000 -3.504 0.000 -3.118

GAS2L3 0.000 -2.011 0.000 -1.785 0.000 -3.946

GATA1 0.000 -2.176 0.000 -2.214 0.000 -2.267

GATA3 0.355 -0.663 0.009 0.586 0.000 2.997

GBAT2 0.000 1.467 0.000 2.781 0.000 1.897

GBP4 0.015 0.587 0.000 2.634 0.008 0.399

GCA 0.000 -2.332 0.000 -4.504 0.000 -4.538

GCLC 0.000 -1.684 0.000 -2.279 0.000 -1.867

144

GCLM 0.000 -2.006 0.000 -2.874 0.000 -2.096

GCNT2 0.000 -1.636 0.000 -2.979 0.000 -2.531

GDF11 0.000 1.489 0.000 2.511 0.000 1.685

GFI1B 0.000 -3.921 0.000 -2.924 0.000 -3.282

GGA2 0.000 1.468 0.001 -0.050 0.000 -2.605

GGH 0.004 -0.886 0.000 -1.977 0.000 -2.319

GGTA1P 0.000 -4.008 0.000 -5.529 0.000 -5.243

GJB6 0.000 -3.035 0.000 -4.755 0.000 -4.511

GJC1 0.000 2.299 0.000 2.099 0.000 1.923

GKAP1 0.001 0.868 0.000 1.518 0.000 2.122

GLIPR2 0.000 -1.824 0.000 -3.842 0.000 -1.649

GLMP 0.000 -1.312 0.000 -2.468 0.000 -2.102

GLT1D1 0.000 -2.194 0.000 -3.795 0.000 -3.296

GMPR 0.000 -2.964 0.000 -3.441 0.000 -3.683

GNA15 0.001 -1.229 0.000 1.616 0.000 2.260

GNAI1 0.581 0.182 0.000 3.344 0.499 -0.182

GNAQ 0.000 -2.467 0.000 -1.172 0.000 0.885

GNAS 0.084 0.159 0.000 2.016 0.000 1.606

GNG11 0.004 -1.580 0.000 0.823 0.000 -3.473

GNG7 0.000 1.099 0.000 2.705 0.000 -2.707

GNLY 0.753 -0.425 0.000 -2.683 0.000 -2.739

GNPDA1 0.062 -0.670 0.000 -2.713 0.151 -0.307

GNS 0.000 -1.436 0.000 -2.877 0.000 -3.125

GPAT3 0.000 -3.252 0.000 -4.652 0.000 -4.395

GPCPD1 0.001 -1.007 0.000 -2.139 0.000 -2.217

145

GPD1L 0.952 -0.048 0.003 -0.507 0.000 -2.447

GPI 0.001 -0.703 0.000 -2.446 0.000 -1.885

GPM6A 0.000 2.701 0.136 0.017 0.398 0.020

GPM6B 0.009 0.630 0.000 2.564 0.000 -0.723

GPR160 0.000 -2.024 0.000 -4.162 0.000 -4.314

GPR176 0.066 -0.163 0.000 2.015 0.000 -0.154

GPR18 0.000 2.104 0.020 0.614 0.014 -0.476

GPR183 0.019 -1.257 0.000 -2.963 0.000 -2.888

GPR27 0.000 -3.139 0.000 -5.632 0.000 -4.481

GPR65 0.387 -0.447 0.000 -2.250 0.000 -3.114

GPR84 0.000 -2.858 0.000 -4.468 0.000 -4.345

GPSM1 0.200 0.516 0.000 3.829 0.000 1.756

GPX3 0.004 -1.521 0.000 -2.409 0.000 -2.497

GRB10 0.000 -2.031 0.000 1.501 0.000 -2.088

GREM1 0.619 0.060 0.000 2.297 0.354 -0.022

GRK3 0.005 0.409 0.000 1.052 0.000 2.193

GRK6 0.000 -1.172 0.000 -2.027 0.000 -1.829

GRN 0.000 -1.291 0.000 -3.357 0.000 -3.591

GSAP 0.052 -1.005 0.588 -0.161 0.000 -3.448

GSN 0.000 -3.066 0.000 -1.400 0.000 -4.474

GSPT2 0.002 1.374 0.000 1.560 0.000 2.312

GSR 0.000 -0.807 0.000 -2.019 0.000 -1.103

GTF2IRD1 0.000 0.886 0.000 2.285 0.022 0.280

GTF3A 0.000 0.610 0.000 1.223 0.000 2.194

GUCY1A3 0.004 -0.983 0.000 3.292 0.000 3.090

146

GXYLT2 0.000 -1.479 0.000 -1.935 0.000 3.622

GYG1 0.000 -1.713 0.000 -3.153 0.000 -3.157

GYPA 0.000 -4.971 0.000 -2.230 0.000 -4.386

GYPB 0.000 -4.701 0.000 -2.723 0.000 -4.436

GYPE 0.000 -2.491 0.000 -2.635 0.000 -2.658

GYS1 0.000 -2.036 0.000 -1.708 0.000 -2.193

GZMA 0.832 0.280 0.000 -1.733 0.000 -2.219

GZMB 1.000 0.007 0.000 -2.648 0.000 -2.504

GZMH 0.745 0.414 0.000 -2.025 0.000 -2.304

GZMK 0.513 0.601 0.000 -1.952 0.000 -2.560

H1F0 0.000 -2.614 0.000 -1.012 0.000 -2.071

H2BFS 0.003 1.188 0.000 2.081 0.000 0.903

H2BFXP 0.000 1.991 0.000 2.859 0.000 4.419

HACD1 0.000 -1.872 0.000 2.224 0.000 1.670

HACD4 0.000 -1.740 0.000 -1.936 0.000 -2.865

HAGH 0.000 -2.007 0.000 -1.843 0.000 -2.189

HAL 0.000 -2.083 0.000 -4.109 0.000 -3.639

HAR1A 0.953 -0.017 0.000 2.268 0.000 1.203

HBD 0.000 -3.413 0.000 -3.365 0.000 -3.825

HBEGF 0.250 0.095 0.001 1.008 0.000 -2.453

HBM 0.000 -4.033 0.000 -3.906 0.000 -4.282

HBQ1 0.000 -2.855 0.000 -3.282 0.000 -3.254

HCAR3 0.022 -1.854 0.000 -3.205 0.000 -3.247

HCK 0.099 -0.820 0.000 -2.902 0.000 -3.912

HDGFRP3 0.001 0.888 0.000 0.824 0.000 2.340

147

HEBP1 0.000 -2.148 0.000 -2.186 0.000 -2.816

HECTD2 0.045 0.185 0.000 2.094 0.000 0.601

HELLS 0.003 0.424 0.000 0.782 0.000 2.055

HEMGN 0.000 -4.660 0.000 -4.307 0.000 -3.528

HES1 0.027 1.161 0.058 -0.229 0.000 2.861

HES4 0.328 0.374 0.002 -0.284 0.000 3.140

HHIP 0.004 0.794 0.000 1.900 0.000 5.012

HHIP-AS1 0.001 1.327 0.000 2.807 0.000 5.584

HIST1H2AM 0.036 0.969 0.000 2.561 0.000 0.911

HIST1H2BC 0.725 -0.289 0.000 2.062 0.005 0.715

HIST1H2BD 0.011 1.198 0.000 2.689 0.000 1.389

HIST1H2BK 0.153 0.476 0.000 2.120 0.000 0.686

HIST3H2A 0.023 0.621 0.000 -2.141 0.007 0.494

HIVEP3 0.001 0.556 0.000 0.990 0.000 2.461

HK3 0.000 -2.318 0.000 -5.554 0.000 -4.587

HLA-DMA 0.001 1.307 0.000 1.616 0.000 -3.373

HLA-DMB 0.000 1.219 0.000 1.390 0.000 -2.274

HLA-DOA 0.000 1.915 0.000 3.669 0.000 -1.146

HLA-DOB 0.000 2.121 0.014 0.564 0.001 -0.481

HLA-DPA1 0.000 2.116 0.000 1.655 0.000 -3.127

HLA-DPB1 0.002 0.970 0.000 1.774 0.000 -3.052

HLA-DQB1 0.003 1.582 0.000 1.575 0.000 -3.128

HLA-DRA 0.084 0.898 0.000 1.407 0.000 -3.516

HLA-DRB4 0.193 -1.965 0.151 0.448 0.000 -2.368

HLA-DRB6 0.001 0.061 0.000 0.548 0.000 -2.020

148

HLX 0.000 -2.517 0.001 -0.666 0.000 -4.540

HMBS 0.000 -2.235 0.000 -3.160 0.000 -2.794

HMCES 0.000 1.086 0.000 2.488 0.000 1.398

HMHB1 0.003 0.466 0.000 4.462 0.000 1.594

HMMR 0.001 -1.626 0.000 -2.022 0.000 -1.980

HMOX1 0.519 -0.548 0.000 -4.440 0.000 -3.702

HNMT 0.000 -1.996 0.000 -4.609 0.000 -4.095

HNRNPLL 0.000 -2.524 0.000 -2.540 0.000 -1.295

HOOK1 0.002 -1.125 0.000 -2.149 0.000 -1.845

HP 0.000 -3.344 0.000 -5.711 0.000 -5.130

HPS4 0.788 -0.096 0.000 2.790 0.000 0.736

HPSE 0.000 -1.546 0.000 -1.743 0.000 -2.089

HRK 0.000 3.457 0.000 2.095 0.000 0.578

HSPA6 0.881 0.142 0.000 -2.466 0.000 -2.445

HSPB1 0.026 0.861 0.000 2.508 0.000 0.851

HSPC324 0.715 -0.025 0.000 2.348 0.279 -0.033

HYAL3 0.000 -1.569 0.000 -2.300 0.000 -2.188

ICA1 0.000 -1.906 0.000 -3.310 0.000 -2.440

ICAM1 0.602 -0.348 0.000 -2.290 0.000 -2.306

ICAM3 0.000 -1.144 0.000 -2.623 0.000 -0.829

ICAM4 0.000 -2.175 0.000 -2.298 0.000 -2.048

ICOSLG 0.000 1.176 0.000 2.899 0.000 -0.450

ID2 0.430 -0.242 0.000 -0.921 0.000 -2.508

ID3 0.000 3.431 0.000 4.225 0.000 1.536

IDS 0.127 -0.496 0.000 -2.010 0.000 -0.903

149

IER3 0.004 -1.802 0.000 -3.214 0.000 -2.978

IFI16 0.001 1.461 0.000 2.191 0.000 1.817

IFNGR1 0.066 -0.742 0.000 -1.578 0.000 -2.108

IFNLR1 0.000 3.063 0.000 1.807 0.726 -0.012

IGF1R 0.000 -2.278 0.000 -1.317 0.001 -0.693

IGF2BP2 0.000 -2.762 0.000 -1.829 0.000 -2.070

IGF2BP3 0.000 3.842 0.000 3.172 0.268 -0.051

IGFBP7 0.000 -2.699 0.000 0.869 0.000 -3.413

IGHD 0.000 -2.011 0.000 -2.215 0.000 -2.457

IGHM 0.000 2.672 0.000 2.761 0.000 -2.862

IGHV5-78 0.000 1.666 0.000 2.428 0.000 -0.298

IGKC 0.000 -3.273 0.000 -3.902 0.000 -4.289

IGLC1 0.001 -1.562 0.000 -2.439 0.000 -2.901

IGLJ3 0.004 -1.156 0.000 -1.962 0.000 -2.304

IGLV1-44 0.029 -0.955 0.000 -1.795 0.000 -2.546

IGSF6 0.011 -1.470 0.000 -4.118 0.000 -3.471

IKZF2 0.000 2.436 0.121 0.516 0.000 4.223

IL10RA 0.968 -0.039 0.000 -2.694 0.000 -2.745

IL13RA1 0.002 -1.336 0.000 -1.030 0.000 -2.689

IL17RA 0.000 -1.396 0.000 -2.296 0.000 -2.207

IL18 0.000 -2.471 0.000 -2.934 0.000 -3.224

IL18R1 0.315 -0.586 0.000 -2.089 0.000 -1.655

IL18RAP 0.000 -2.171 0.000 -4.985 0.000 -4.263

IL1B 0.568 0.496 0.055 0.529 0.000 -2.059

IL1R1 0.000 -2.007 0.000 -3.792 0.000 -3.710

150

IL1R2 0.658 -0.657 0.000 -3.234 0.000 -3.301

IL1RN 0.001 -2.134 0.000 -4.723 0.000 -4.204

IL2RB 0.119 -1.059 0.000 -2.797 0.000 -2.444

IL6R 0.000 -2.642 0.000 -2.968 0.000 -3.348

ILDR2 0.070 0.012 0.435 0.006 0.000 2.066

IMPA2 0.000 -1.018 0.000 -3.201 0.000 -2.504

IMPACT 0.004 -0.815 0.000 -1.764 0.000 -3.279

INHBA 0.000 -2.261 0.000 -4.475 0.000 -3.411

INO80C 0.270 0.198 0.000 3.060 0.000 -0.651

INPP1 0.000 -2.236 0.000 -2.917 0.000 -1.947

INSR 0.030 -0.742 0.000 2.803 0.000 -1.154

INTS3 0.440 0.038 0.000 2.160 0.005 0.488

IRAK2 0.004 -1.505 0.000 -1.471 0.000 -2.647

IRAK3 0.000 -2.113 0.000 -2.053 0.000 -3.830

IRF8 1.000 0.006 0.159 0.314 0.000 -2.147

IRS2 0.000 -1.486 0.000 -1.045 0.000 -3.528

IRX1 0.349 -0.117 0.000 3.002 0.245 0.275

IRX3 0.528 -0.209 0.000 1.044 0.000 2.599

IRX5 0.027 0.500 0.000 0.669 0.000 3.804

ITGA2B 0.000 -2.217 0.000 -2.722 0.000 -2.469

ITGA6 0.000 -1.760 0.000 2.239 0.028 0.613

ITGA9 0.000 -2.020 0.000 -1.430 0.000 -2.605

ITGAM 0.000 -2.803 0.000 -4.524 0.000 -3.709

ITGAV 0.049 -0.841 0.000 -2.322 0.000 -1.451

ITGB2 0.000 -1.944 0.000 -4.019 0.000 -2.813

151

ITGB2-AS1 0.000 -1.883 0.000 -2.687 0.000 -1.548

ITGB3 0.000 -1.996 0.000 -2.244 0.000 -2.207

ITK 0.211 -0.973 0.000 -2.470 0.015 0.646

ITM2A 0.012 -1.054 0.000 -1.745 0.000 2.553

ITPR2 0.000 1.361 0.003 -0.235 0.000 2.032

ITPR3 0.000 2.004 0.001 0.806 0.000 1.493

ITPRIP 0.004 -1.445 0.000 -1.601 0.000 -2.134

IVNS1ABP 0.000 -2.332 0.000 -2.259 0.000 -1.907

JADE3 0.000 0.919 0.000 3.023 0.000 2.088

JAG1 0.001 -1.590 0.000 -3.002 0.000 -3.779

JAK2 0.000 -2.095 0.000 -1.036 0.000 -1.364

JAML 0.504 -0.577 0.000 -3.282 0.000 -2.631

JAZF1 0.001 -1.051 0.000 -3.075 0.000 -2.971

JCHAIN 0.835 -0.210 0.000 -1.536 0.000 -3.717

JDP2 0.008 -0.928 0.000 -2.764 0.000 -3.274

JUP 0.629 0.290 0.000 4.503 0.009 0.735

KANK2 0.088 -0.890 0.018 0.604 0.000 -3.192

KAT2B 0.000 -2.006 0.000 -1.997 0.000 -1.987

KBTBD11 0.000 -2.693 0.000 -1.831 0.000 -2.246

KBTBD6 0.000 -1.951 0.000 -2.812 0.000 -1.066

KBTBD7 0.000 -1.751 0.000 -2.382 0.000 -1.267

KCNA3 0.032 0.563 0.000 0.632 0.000 2.338

KCNE3 0.000 -2.078 0.000 -3.447 0.000 -4.537

KCNH2 0.000 -2.464 0.000 -4.420 0.000 -3.337

KCNJ15 0.011 -1.712 0.000 -3.843 0.000 -3.616

152

KCNK12 0.000 0.733 0.000 3.520 0.000 0.873

KCNK17 0.824 0.027 0.000 1.186 0.000 2.953

KCNK5 0.000 -1.319 0.000 -2.645 0.043 -0.396

KCNQ1 0.000 -1.115 0.000 -2.083 0.000 -2.128

KCTD1 0.002 0.148 0.000 0.675 0.000 4.166

KCTD12 0.004 -1.556 0.000 -2.710 0.000 -2.057

KEL 0.000 -3.495 0.000 -3.751 0.000 -3.744

KHDRBS3 0.006 0.463 0.000 2.331 0.000 0.714

KIAA0368 0.153 -0.416 0.000 -0.566 0.000 -2.277

KIAA0485 0.007 -1.618 0.000 -2.082 0.000 -1.950

KIAA0513 0.000 -1.800 0.000 -3.485 0.000 -2.899

KIAA1211 0.011 -1.063 0.000 -2.498 0.000 -1.661

KIAA1211L 0.000 -2.267 0.000 -1.662 0.000 -2.511

KIF13A 0.000 -1.956 0.000 -1.467 0.000 -3.253

KIF16B 0.173 -0.409 0.000 2.174 0.000 -0.912

KIF1B 0.000 -2.160 0.000 -1.849 0.000 -2.826

KIF21B 0.016 -0.898 0.000 -2.346 0.000 -0.954

KIF3A 0.000 1.119 0.000 0.681 0.000 2.721

KIT 0.002 -1.447 0.000 -2.727 0.000 -1.959

KLF1 0.000 -4.268 0.000 -4.577 0.000 -4.646

KLF11 0.145 0.270 0.001 -0.970 0.000 -2.983

KLF3 0.000 -2.777 0.000 -0.890 0.000 -3.207

KLF4 0.001 -1.507 0.000 -2.310 0.000 -2.926

KLF5 0.000 -3.051 0.000 -4.723 0.000 -3.699

KLF8 0.825 0.011 0.000 2.497 0.005 0.348

153

KLF9 0.028 0.086 0.000 0.464 0.000 -3.082

KLHL2 0.708 -0.216 0.030 0.454 0.000 -2.002

KLHL24 0.068 0.540 0.000 2.266 0.000 1.087

KLHL6 0.003 0.344 0.000 2.485 0.000 1.174

KLRB1 0.021 -1.519 0.000 -2.866 0.000 -1.716

KLRD1 0.222 -0.781 0.000 -2.348 0.000 -2.279

KLRF1 0.653 -0.481 0.000 -2.107 0.000 -2.196

KRT1 0.004 -2.394 0.000 -2.214 0.722 -0.194

KYNU 0.034 0.730 0.000 -1.920 0.000 -2.622

LACTB 0.011 -1.185 0.000 -2.482 0.000 -2.278

LAIR1 0.000 -2.229 0.027 -0.384 0.000 -1.108

LAMC1 0.000 1.092 0.000 2.541 0.000 -0.294

LAMP1 0.000 -1.829 0.000 -1.548 0.000 -2.058

LAMP2 0.000 -2.028 0.000 -2.660 0.000 -2.236

LAPTM4B 0.134 -0.733 0.000 -2.297 0.000 -2.753

LAPTM5 0.108 -0.497 0.000 0.365 0.000 -2.060

LARGE1 0.000 0.687 0.000 2.014 0.511 0.003

LAT 0.020 -1.228 0.000 -2.506 0.000 2.220

LATS2 0.001 -1.202 0.055 0.496 0.000 -2.881

LCK 0.474 0.694 0.000 -0.849 0.000 3.761

LCN10 0.529 -0.099 0.000 2.995 0.605 -0.049

LCN2 0.000 -3.803 0.000 -6.428 0.000 -5.877

LCN6 0.798 -0.018 0.000 2.179 0.836 0.012

LDLRAD4 0.001 0.963 0.000 3.118 0.000 2.830

LDLRAP1 0.054 -0.865 0.000 -0.448 0.000 -2.298

154

LDOC1L 0.973 0.012 0.000 3.722

LEF1 0.028 0.906 0.000 3.648 0.000 3.760

LEF1-AS1 0.253 0.126 0.000 4.021 0.000 2.813

LEPROT 0.000 -1.396 0.000 -1.696 0.000 -2.054

LGALS1 0.110 -0.603 0.000 -2.668 0.000 -1.914

LGALS12 0.000 -2.218 0.000 -3.613 0.000 -2.743

LGALS2 0.277 -0.795 0.000 -2.658 0.000 -2.106

LGALS3 0.000 -2.325 0.000 -4.662 0.000 -4.227

LGALS3BP 0.983 -0.025 0.000 1.880 0.000 2.421

LGALSL 0.000 -3.098 0.000 -4.498 0.000 -4.277

LGR5 0.055 0.438 0.000 2.332 0.178 0.159

LHFPL2 0.116 -0.648 0.000 -2.026 0.000 -2.018

LIG4 0.190 0.480 0.000 2.301 0.000 1.785

LILRA2 0.000 -1.714 0.000 -0.918 0.000 -2.476

LILRA3 0.598 -0.515 0.000 -4.875 0.000 -3.951

LILRA5 0.588 -0.369 0.000 -3.249 0.000 -2.712

LILRA6 0.122 -0.528 0.000 -1.546 0.000 -2.221

LILRB1 0.364 0.390 0.000 -0.910 0.000 -2.295

LILRB2 0.604 -0.408 0.000 -1.578 0.000 -4.083

LIMD1 0.007 0.120 0.000 2.382 0.000 1.013

LIN7A 0.000 -3.243 0.000 -4.662 0.000 -4.056

LINC00342 0.468 0.263 0.000 0.796 0.000 2.323

LINC00642 0.333 0.123 0.000 2.719 0.427 0.017

LINC00865 0.042 0.246 0.000 3.133 0.000 1.608

LINC01013 0.050 0.056 0.000 5.055 0.011 -0.176

155

LINC01120 0.791 -0.011 0.364 0.058 0.000 2.704

LINC01215 0.000 1.686 0.000 3.006 0.000 3.027

LINC01260 0.033 0.260 0.116 0.105 0.000 3.302

LINC01268 0.000 -2.117 0.000 -2.955 0.000 -2.262

LINC01550 0.375 -0.064 0.002 -0.210 0.000 2.812

LITAF 0.010 -0.984 0.000 -2.324 0.000 -2.311

LMF1 0.084 0.144 0.000 2.131 0.000 0.700

LMNA 0.000 -1.820 0.000 -2.097 0.000 -2.685

LMO2 0.000 -1.920 0.000 -1.728 0.000 -2.341

LNPK 0.000 -1.827 0.000 -2.259 0.000 -1.253

LOC10004971 6 0.000 -2.167 0.000 -0.942 0.000 -1.301

LOC10012944 7 0.000 2.533 0.000 1.545 0.004 0.690

LOC10013045 8 0.000 1.662 0.000 5.041 0.795 0.026

LOC10013047 6 0.894 -0.004 0.000 2.200 0.000 2.621

LOC10050550 1 0.206 0.394 0.000 2.815 0.000 4.590

LOC10050556 4 0.295 0.259 0.000 2.773 0.000 -0.511

LOC10106039 1 0.570 0.056 0.000 2.225 0.000 3.468

LOC10192800 0 0.000 1.492 0.000 1.024 0.000 2.171

LOC10192804 7 0.000 -1.899 0.000 -2.256 0.000 -2.240

LOC10192842 9 0.000 -2.267 0.000 -2.840 0.000 -3.901

156

LOC10192884 1 0.000 0.401 0.000 2.473 0.000 -0.398

LOC10260646 5 0.002 0.698 0.000 1.784 0.000 2.464

LOC10537727 6 0.246 -0.544 0.000 -2.080 0.000 -1.848

LOC202181 0.011 0.752 0.000 2.070 0.000 1.016

LOC285097 0.181 0.338 0.000 3.398 0.000 2.552

LOC643072 0.000 -1.882 0.000 -1.056 0.000 -2.133

LOC728175 0.000 2.061 0.502 0.056

LOXL3 0.000 -1.645 0.000 -2.422 0.000 -2.308

LPAR6 0.064 -0.833 0.000 3.091 0.000 2.874

LPCAT2 0.000 -2.814 0.000 -2.651 0.000 -2.852

LRG1 0.000 -1.665 0.000 -3.390 0.000 -3.074

LRMP 0.000 2.089 0.000 2.652 0.000 1.511

LRP10 0.004 -0.718 0.000 -1.151 0.000 -2.357

LRP12 0.320 -0.157 0.053 -0.185 0.000 3.466

LRP3 0.000 -1.793 0.000 -1.963 0.000 -2.393

LRP5 0.740 0.046 0.000 2.026 0.000 0.619

LRP6 0.000 0.592 0.000 2.762 0.000 1.202

LRP8 0.000 0.537 0.000 1.356 0.000 2.071

LRRC1 0.683 0.123 0.000 0.657 0.000 2.483

LRRC25 0.724 -0.257 0.000 -3.068 0.000 -2.342

LRRC4 0.000 -2.270 0.000 -2.830 0.000 -2.907

LRRC75A 0.000 -1.938 0.000 -2.425 0.000 -1.965

LRRC8C 0.445 -0.185 0.000 2.668 0.000 -1.012

LRRK2 0.111 -1.246 0.000 -4.342 0.000 -3.607

157

LSP1 0.159 -0.595 0.000 -2.151 0.000 -1.528

LST1 0.000 -1.465 0.000 -2.128 0.000 -2.303

LTA4H 0.000 -1.139 0.000 -2.353 0.000 -2.039

LTB4R 0.000 -1.515 0.000 -1.488 0.000 -2.080

LTBP1 0.002 -1.252 0.000 -3.284 0.000 -2.365

LTF 0.000 -3.875 0.000 -5.811 0.000 -5.282

LUNAR1 0.140 -0.186 0.026 -0.101 0.000 2.021

LXN 0.000 -2.093 0.000 -1.189 0.000 -2.102

LY86 0.099 0.707 0.800 0.066 0.000 -2.936

LYN 0.005 0.837 0.000 -2.072 0.000 -2.697

LYST 0.000 -2.025 0.000 -2.270 0.000 -2.891

LYZ 0.000 -2.862 0.000 -6.035 0.000 -4.817

LZTFL1 0.037 0.169 0.000 0.591 0.000 3.030

MACF1 0.005 -0.697 0.000 2.006 0.000 1.937

MACROD2 0.209 0.423 0.000 4.919 0.459 -0.015

MAF 0.036 -1.024 0.000 -2.247 0.000 -2.064

MAFB 0.874 0.142 0.000 -4.278 0.000 -3.048

MAGED1 0.000 1.164 0.000 1.635 0.000 2.181

MAL 0.000 -1.912 0.000 -3.091 0.000 2.933

MAN1A1 0.073 -0.846 0.075 -0.468 0.000 -2.149

MANSC1 0.000 -2.450 0.000 -3.929 0.000 -3.616

MAP1A 0.170 0.254 0.000 1.640 0.000 3.795

MAP3K13 0.000 -1.223 0.000 -3.718 0.000 -4.523

MAP3K5 0.000 -1.078 0.000 -1.193 0.000 -2.269

MAP3K7CL 0.057 -1.267 0.000 -2.029 0.000 -2.093

158

MAP3K8 0.002 -1.566 0.000 -1.680 0.000 -2.265

MAP7 0.000 -2.359 0.000 -2.969 0.000 -2.852

MAPK12 0.000 2.244 0.001 0.491 0.295 0.078

MAPK14 0.000 -1.724 0.000 -2.134 0.000 -1.768

MAPKAPK3 0.000 -1.057 0.000 -2.136 0.000 -0.761

MAPKBP1 0.002 0.736 0.000 2.982 0.000 1.466

1-Mar 0.000 -3.104 0.000 -4.798 0.000 -4.164

1-Mar 0.663 0.036 0.000 -2.477 0.000 -1.991

2-Mar 0.000 -1.636 0.000 -2.126 0.000 -2.246

3-Mar 0.000 -2.035 0.017 0.218 0.000 -2.529

MARCKS 0.000 -1.582 0.000 -2.525 0.000 -3.987

MARCKSL1 0.000 2.124 0.000 2.089 0.000 2.529

MAST4 0.087 -0.611 0.000 0.878 0.000 2.352

MB21D1 0.021 -1.071 0.000 0.410 0.000 -2.184

MBOAT1 0.000 -2.442 0.000 -1.068 0.558 -0.110

MBOAT2 0.000 -2.777 0.000 -3.300 0.000 -3.451

MBP 0.000 -1.094 0.000 -0.816 0.000 -2.099

MCEMP1 0.000 -2.656 0.000 -4.396 0.000 -4.662

MCL1 0.003 -1.267 0.000 -2.028 0.000 -2.282

MCOLN2 0.000 3.514 0.000 -1.093 0.000 -1.125

MCTP1 0.000 -2.313 0.000 -2.533 0.000 -1.963

MDK 0.965 -0.014 0.000 3.030 0.000 0.992

MDM2 0.000 1.243 0.000 2.087 0.000 0.531

MED17 0.000 1.007 0.000 1.710 0.000 2.488

MEF2A 0.722 -0.173 0.000 2.085 0.006 0.390

159

MEF2C 0.470 -0.510 0.000 3.732 0.000 -2.193

MEGF9 0.000 -1.926 0.000 -2.557 0.000 -2.375

METRNL 0.944 0.072 0.004 -0.680 0.000 -2.289

METTL7A 0.747 -0.149 0.000 -0.721 0.000 -2.503

MEX3B 0.372 0.113 0.000 1.169 0.000 2.966

MEX3C 0.011 1.498 0.000 0.969 0.000 2.067

MFAP3L 0.000 -2.801 0.000 -3.270 0.000 -3.239

MFSD1 0.000 -1.499 0.000 -1.821 0.000 -2.249

MGA 0.155 0.837 0.000 1.601 0.000 2.035

MGAM 0.000 -2.825 0.000 -5.171 0.000 -4.373

MGLL 0.000 -1.188 0.000 -2.579 0.000 -1.699

MGST1 0.000 -2.681 0.000 -6.142 0.000 -5.285

MGST3 0.000 -2.095 0.000 -2.294 0.000 -2.639

MICAL2 0.000 -2.708 0.000 -2.225 0.000 -2.079

MICALL2 0.000 -2.366 0.000 -2.708 0.000 -2.411

MINPP1 0.000 -2.780 0.000 -3.779 0.000 -3.428

MITF 0.000 -1.171 0.000 -2.161 0.000 -1.859

MKL2 0.013 0.299 0.000 1.644 0.000 2.199

MLC1 0.000 -2.781 0.000 -2.639 0.000 -3.113

MLLT11 0.000 1.420 0.000 3.033 0.000 4.891

MLLT3 0.046 -1.268 0.000 -2.447 0.447 0.127

MLXIP 0.111 -0.493 0.000 2.368 0.000 -0.395

MME 0.034 1.531 0.000 4.534 0.067 0.741

MMP25 0.000 -1.862 0.000 -3.047 0.000 -2.998

MMP28 0.116 0.101 0.000 2.222 0.000 -0.311

160

MMP8 0.000 -5.011 0.000 -6.999 0.000 -6.477

MMP9 0.000 -3.699 0.000 -6.339 0.000 -5.374

MNDA 0.000 -2.658 0.000 -6.054 0.000 -5.465

MOB3B 0.111 0.133 0.000 2.523 0.005 0.102

MOSPD1 0.000 -1.850 0.000 -1.812 0.000 -2.134

MOV10 0.001 0.601 0.000 2.197 0.000 1.516

MPEG1 0.789 -0.291 0.000 -4.587 0.000 -4.301

MPO 0.000 -4.023 0.000 -6.272 0.000 -6.210

MPP1 0.000 -2.526 0.000 -0.808 0.000 -1.148

MPP7 0.000 -2.393 0.000 -3.263 0.000 -1.288

MPZL3 0.001 -1.340 0.000 -3.111 0.000 -1.996

MRC1 0.071 -0.846 0.000 2.168 0.051 -0.437

MS4A1 0.000 2.192 0.001 0.757 0.000 -2.145

MS4A3 0.000 -4.779 0.000 -6.740 0.000 -6.398

MS4A4A 0.026 0.700 0.000 -2.012 0.000 -2.745

MS4A6A 0.000 -2.902 0.000 -3.479 0.000 -3.474

MS4A7 0.649 0.260 0.000 -2.929 0.000 -2.832

MSI2 0.000 1.630 0.000 3.186 0.000 3.347

MSRA 0.000 -2.186 0.000 -1.271 0.000 -3.570

MSRB1 0.000 -2.196 0.000 -4.310 0.000 -4.001

MSRB3 0.000 -3.441 0.000 -4.080 0.000 -2.697

MTCL1 0.000 2.363 0.000 2.930 0.000 1.123

MTMR11 0.474 -0.381 0.000 -2.166 0.000 -0.899

MXD1 0.000 -1.887 0.000 -3.820 0.000 -1.939

MXI1 0.000 -1.303 0.000 -2.090 0.000 -2.318

161

MXRA7 0.047 -1.018 0.000 2.228 0.002 0.327

MYADM 0.000 -2.123 0.000 -1.542 0.000 -2.228

MYC 0.000 2.173 0.470 -0.165 0.124 -0.429

MYEF2 0.000 1.396 0.000 1.082 0.000 3.525

MYL4 0.000 -3.088 0.000 -3.624 0.000 -3.706

MYL6B 0.000 1.176 0.000 2.410 0.000 2.470

MYLK 0.003 -1.720 0.000 2.198 0.000 -1.821

MYO10 0.075 0.092 0.000 2.217 0.000 -0.646

MYO1B 0.063 0.093 0.000 2.523 0.000 1.742

MYO1F 0.000 -1.531 0.000 -3.024 0.000 -3.037

MYO5C 0.754 0.089 0.000 4.564 0.000 1.085

MYO7B 0.431 0.029 0.000 0.498 0.000 4.531

MYOF 0.719 -0.352 0.000 -1.492 0.000 -2.190

MYRIP 0.752 -0.012 0.000 2.709 0.415 0.073

MYT1L 0.111 0.062 0.000 1.286 0.000 2.041

MZB1 0.000 1.816 0.000 2.985 0.000 3.136

N4BP2 0.154 0.488 0.000 1.845 0.000 2.423

NA 0.000 5.010 0.000 -3.598 0.000 -3.326

NAALADL1 0.000 -1.309 0.000 -2.007 0.000 -1.598

NACC2 0.054 -1.259 0.000 -3.567 0.000 -2.849

NADK 0.155 -0.651 0.000 -1.990 0.000 -2.296

NAGA 0.000 -1.313 0.000 -2.274 0.000 -2.840

NAMPT 0.130 -1.026 0.000 -3.812 0.000 -3.059

NARFL 0.000 0.664 0.000 2.006 0.000 0.613

NAT14 0.000 1.465 0.000 1.171 0.000 2.699

162

NATD1 0.000 -2.050 0.000 -2.634 0.000 -2.717

NAV1 0.000 0.646 0.000 4.320 0.201 -0.137

NCEH1 0.000 -2.278 0.000 -3.233 0.000 -2.959

NCF2 0.000 -2.417 0.000 -3.837 0.000 -4.798

NCF4 0.000 -1.456 0.000 -1.744 0.000 -4.473

NDFIP1 0.000 -1.319 0.000 -3.046 0.000 -1.165

NDST3 0.053 0.053 0.713 0.004 0.000 2.775

NEGR1 0.028 -0.600 0.000 2.261 0.000 1.344

NEK6 0.000 -1.512 0.000 -1.041 0.000 -3.252

NELL2 0.133 -1.117 0.000 -2.264 0.000 -2.468

NEURL1B 0.000 -2.169 0.000 2.143 0.086 -0.366

NFAM1 0.000 -1.609 0.000 -3.563 0.000 -3.107

NFE2 0.000 -3.861 0.000 -3.910 0.000 -3.012

NFIA 0.000 -2.921 0.000 -2.855 0.000 -2.717

NFKBID 0.427 -0.488 0.000 -1.444 0.000 -2.280

NINJ2 0.000 -2.987 0.000 -3.435 0.000 -2.055

NINL 0.001 0.390 0.000 1.651 0.000 2.808

NIPAL2 0.003 0.153 0.000 -2.267 0.000 -1.469

NKG7 0.000 -1.678 0.000 -3.015 0.000 -3.303

NKX3-1 0.667 0.134 0.116 0.022 0.000 2.473

NLGN4X 0.205 0.049 0.000 1.471 0.000 2.495

NLRC4 0.002 -2.020 0.000 -3.705 0.000 -3.236

NLRP12 0.000 -2.014 0.000 -2.526 0.000 -2.601

NLRP3 0.034 -1.236 0.000 -2.548 0.000 -2.114

NME8 0.000 -2.552 0.000 -3.629 0.000 -3.265

163

NMNAT3 0.000 -1.920 0.000 -2.135 0.000 -1.808

NMT2 0.389 -0.435 0.000 1.565 0.000 2.394

NMU 0.005 -1.831 0.000 -3.231 0.000 -3.144

NNMT 0.000 -2.260 0.000 -2.294 0.000 -2.408

NOCT 0.015 -1.294 0.000 -2.453 0.000 -1.805

NOD2 0.000 -1.291 0.000 -3.130 0.000 -3.125

NOTCH3 0.258 0.098 0.000 -0.127 0.000 2.454

NPC2 0.032 -0.614 0.000 -1.154 0.000 -2.023

NPL 0.000 -2.573 0.000 -3.672 0.000 -4.249

NPR1 0.658 -0.018 0.000 3.462 0.254 -0.116

NPTX2 0.228 0.169 0.012 0.613 0.000 2.128

NPY 0.233 -0.137 0.000 5.231 0.588 0.092

NQO2 0.000 -1.975 0.000 -3.901 0.000 -2.169

NR3C1 0.000 -1.066 0.000 2.260 0.000 1.174

NR4A2 0.000 -2.042 0.000 -2.018 0.000 -3.038

NREP 0.125 0.536 0.000 2.440 0.000 4.490

NRGN 0.000 -2.465 0.000 -3.713 0.000 -4.298

NRIP1 0.006 -1.285 0.000 2.009 0.003 -0.707

NRN1 0.076 0.629 0.000 3.124 0.001 0.711

NRXN2 0.002 0.193 0.000 2.613 0.000 2.983

NRXN3 0.723 -0.019 0.000 2.096 0.064 0.134

NSG1 0.008 0.854 0.008 0.569 0.000 2.227

NT5E 0.389 0.086 0.000 3.613 0.037 0.305

NTNG2 0.000 -2.000 0.000 -2.322 0.000 -0.878

NUCB2 0.000 -2.204 0.000 -1.962 0.000 2.078

164

NUDT11 0.000 1.681 0.002 0.766 0.000 2.106

NUDT19 0.000 -0.936 0.000 -2.490 0.000 -2.197

NUDT5 0.050 0.948 0.000 2.286 0.000 2.135

NXF3 0.000 -1.683 0.000 -2.089 0.000 -1.635

OAS1 0.004 2.171 0.212 0.400 0.002 -0.731

OAT 0.000 -1.557 0.000 -2.476 0.000 -2.481

OCIAD2 0.000 1.737 0.030 -0.442 0.000 2.498

OGFRL1 0.216 -0.640 0.574 0.098 0.000 -2.483

OGN 0.039 0.027 0.041 -0.009 0.000 2.456

OLFM4 0.001 -2.659 0.000 -6.057 0.000 -6.060

OLR1 0.000 -3.970 0.000 -4.960 0.000 -4.588

ORM1 0.000 -4.796 0.000 -6.200 0.000 -5.359

OSBP2 0.000 -1.759 0.000 -2.213 0.000 -2.452

OSBPL10 0.000 2.667 0.000 0.840 0.000 -0.645

OSBPL1A 0.000 -3.432 0.000 -2.429 0.000 -3.505

OSCAR 0.000 -1.756 0.000 -3.910 0.000 -3.273

OSM 0.000 -1.713 0.000 -2.352 0.000 -2.213

P2RX5 0.000 2.875 0.000 1.530 0.000 2.417

P2RY13 0.000 -2.105 0.000 -5.427 0.000 -4.626

P2RY14 0.265 -0.578 0.000 2.239 0.000 -0.709

P2RY2 0.000 -2.332 0.000 -3.717 0.000 -3.418

P4HA2 0.038 0.847 0.000 2.046 0.000 -0.310

PABPC4L 0.611 -0.019 0.004 0.603 0.000 2.730

PADI2 0.000 -1.780 0.000 -3.758 0.000 -3.557

PADI4 0.000 -3.877 0.000 -4.696 0.000 -4.595

165

PAK1 0.000 -1.774 0.000 -2.159 0.000 -2.336

PAN3-AS1 0.953 -0.018 0.000 3.436 0.000 1.537

PAPSS2 0.106 0.316 0.000 -2.424 0.000 -1.664

PAQR9 0.000 -2.911 0.000 -2.930 0.000 -3.099

PARD3 0.021 0.254 0.000 2.675 0.000 3.706

PARP15 0.001 0.902 0.000 2.069 0.002 0.233

PARVB 0.000 -1.788 0.000 -2.704 0.000 -3.306

PATL1 0.002 -1.459 0.000 -2.041 0.000 -1.839

PAWR 0.000 2.366 0.012 0.237 0.000 1.145

PAX5 0.000 2.819 0.000 4.395 0.000 -1.817

PBX1 0.000 -1.485 0.000 -1.636 0.000 -2.143

PCAT18 0.388 0.024 0.029 0.221 0.000 4.165

PCDH10 0.684 0.029 0.091 0.250 0.000 2.397

PCDH9 0.000 3.451 0.000 3.369 0.000 2.028

PCMT1 0.000 -1.391 0.000 -2.150 0.000 -1.571

PCNX2 0.102 0.077 0.000 0.723 0.000 2.564

PCOLCE2 0.000 -3.700 0.000 -5.049 0.000 -4.664

PDE4B 0.537 -0.525 0.001 0.699 0.000 -2.805

PDE4D 0.000 -1.791 0.000 -2.065 0.000 -2.133

PDGFC 0.000 -2.551 0.000 -4.103 0.000 -3.511

PDK4 0.038 0.100 0.000 -1.638 0.000 -2.267

PDLIM1 0.000 2.014 0.000 2.769 0.000 -2.316

PDXK 0.000 -1.177 0.000 -1.867 0.000 -3.193

PDZD8 0.000 -2.294 0.000 -2.920 0.000 -2.215

PDZK1IP1 0.000 -2.703 0.000 -3.215 0.000 -3.127

166

PECR 0.000 -1.961 0.000 -2.574 0.000 -1.990

PEG10 0.000 4.759 0.000 2.204 0.101 -0.130

PELI1 0.034 0.805 0.000 -2.155 0.000 -1.176

PELO 0.042 0.091 0.000 -2.019 0.000 0.853

PEX5L 0.062 0.060 0.588 -0.005 0.000 3.935

PF4 0.000 -4.933 0.000 -6.493 0.000 -6.379

PF4V1 0.000 -2.539 0.000 -2.964 0.000 -2.972

PFKFB3 0.005 -1.343 0.000 -1.813 0.000 -2.888

PFKFB4 0.002 -1.153 0.000 -2.335 0.000 -2.740

PFKP 0.000 1.789 0.000 1.757 0.000 2.660

PGD 0.000 -1.682 0.000 -2.845 0.000 -2.319

PGGHG 0.151 0.777 0.000 1.273 0.000 2.891

PGLYRP1 0.000 -4.298 0.000 -6.438 0.000 -5.823

PGM1 0.018 -0.846 0.000 -2.528 0.000 -1.114

PHACTR1 0.006 1.493 0.000 1.250 0.000 -2.081

PHACTR2 0.000 -2.040 0.000 -1.606 0.000 -2.270

PHC1 0.000 1.140 0.000 2.345 0.000 2.458

PHGDH 0.000 2.047 0.938 0.022 0.000 2.375

PHLDA1 0.504 0.083 0.000 -2.422 0.000 -1.673

PHLPP1 0.105 0.043 0.000 2.425 0.049 -0.388

PIGK 0.000 -1.594 0.000 -1.924 0.000 -2.337

PIK3AP1 0.477 0.348 0.000 1.000 0.000 -2.290

PIK3C2B 0.000 1.596 0.000 3.296 0.000 3.254

PIK3CB 0.000 -1.834 0.000 -2.786 0.000 -1.957

PIK3R3 0.001 1.125 0.000 0.857 0.000 2.450

167

PILRA 0.528 -0.402 0.000 -3.252 0.000 -2.532

PIP4K2A 0.000 -1.556 0.000 -2.130 0.000 -1.734

PIP4K2C 0.520 -0.166 0.000 -3.115 0.000 -1.002

PIP5K1B 0.000 -1.810 0.000 -2.504 0.000 -4.405

PIR 0.000 -1.765 0.000 -2.169 0.000 -1.503

PITPNM2 0.000 1.508 0.000 0.863 0.000 2.180

PIWIL4 0.000 -3.123 0.000 -2.829 0.000 -2.611

PKD2 0.012 -0.712 0.000 2.042 0.000 0.608

PKIA 0.003 0.742 0.002 -0.813 0.000 2.415

PKLR 0.000 -2.234 0.000 -2.613 0.000 -2.619

PKP2 0.000 -1.783 0.000 -2.600 0.000 -2.525

PLA2G4A 0.000 -1.937 0.000 -2.642 0.000 -2.680

PLAC8 0.010 -0.965 0.000 -1.851 0.000 -2.175

PLAGL1 0.000 -1.745 0.000 -2.350 0.000 -1.806

PLAUR 0.003 -1.005 0.000 -2.456 0.000 -2.428

PLBD1 0.000 -2.786 0.000 -6.982 0.000 -5.572

PLBD2 0.027 -0.544 0.000 -2.373 0.000 -2.236

PLCB4 0.939 0.012 0.001 0.799 0.000 2.912

PLCG1 0.002 1.083 0.013 0.436 0.000 3.104

PLCG2 0.001 1.207 0.000 -0.176 0.000 -2.076

PLCL2 0.718 -0.082 0.000 -0.619 0.000 -2.172

PLD1 0.000 -1.818 0.000 -1.531 0.000 -3.305

PLEK 0.000 -1.494 0.000 -3.321 0.000 -3.442

PLEK2 0.001 -1.975 0.000 -2.393 0.000 -2.383

PLEKHA5 0.084 0.218 0.000 1.975 0.000 2.851

168

PLEKHG1 0.000 1.598 0.000 2.488 0.000 1.074

PLEKHG4B 0.000 1.445 0.000 3.419 0.000 2.025

PLEKHO2 0.018 -0.821 0.000 -2.100 0.000 -2.280

PLPP3 0.001 -1.332 0.000 -2.479 0.000 -2.207

PLS1 0.000 -1.770 0.000 -2.823 0.000 -0.940

PLSCR1 0.000 -1.908 0.000 -1.965 0.000 -2.666

PLXDC2 0.000 -1.308 0.000 -2.106 0.000 -2.242

PLXNC1 0.000 -1.856 0.000 -1.326 0.000 -2.598

PMAIP1 0.000 2.214 0.000 2.463 0.000 2.041

PMP22 0.000 -1.407 0.000 -2.245 0.000 -1.726

PNKD 0.043 -0.957 0.000 -2.575 0.000 -1.572

PNP 0.000 -1.862 0.000 -1.860 0.000 -2.775

PNPLA4 0.370 0.119 0.000 -1.263 0.000 -2.138

POMGNT2 0.000 -2.016 0.000 -2.221 0.000 -0.667

POU2AF1 0.000 2.733 0.000 3.161 0.005 -0.855

POU4F1 0.000 1.541 0.000 3.823 0.003 0.613

PPBP 0.000 -4.884 0.000 -7.009 0.000 -6.968

PPFIBP2 0.000 2.100 0.000 -1.347 0.000 -1.554

PPIF 0.018 -1.264 0.000 -2.521 0.000 -1.964

PPM1M 0.000 -1.420 0.000 -2.275 0.000 -1.858

PPP1R16B 0.000 1.631 0.000 2.452 0.000 1.820

PPP1R3D 0.000 -2.142 0.000 -1.523 0.000 -2.923

PPP2R2C 0.044 0.074 0.000 2.091 0.051 0.039

PPP3CA 0.006 -0.813 0.000 0.637 0.000 -2.201

PPP3CC 0.006 0.819 0.000 2.395 0.000 0.465

169

PRAM1 0.000 -2.452 0.000 -4.284 0.000 -3.379

PRCD 0.010 0.405 0.000 2.859 0.000 0.895

PRCP 0.000 -1.115 0.000 -2.294 0.000 -2.288

PRDM15 0.000 2.669 0.000 1.457 0.000 1.292

PRDX1 0.000 1.463 0.000 2.363 0.000 0.665

PREX1 0.000 -1.281 0.003 0.450 0.000 -2.075

PRG2 0.000 -3.346 0.000 -5.327 0.000 -4.947

PRG3 0.000 -4.864 0.000 -5.815 0.000 -5.320

PRKAR2B 0.000 -1.557 0.000 -2.569 0.000 -3.530

PRKCD 0.004 -1.111 0.000 -2.712 0.000 -3.490

PRKCE 0.000 1.182 0.000 3.114 0.000 0.682

PRKCQ 0.001 -1.587 0.000 -0.782 0.000 2.564

PRKRA 0.112 0.592 0.000 1.593 0.000 2.786

PRKX 0.017 1.176 0.000 2.585 0.000 0.683

PROK2 0.000 -2.868 0.000 -4.974 0.000 -4.284

PRRG4 0.000 -1.965 0.000 -4.327 0.000 -4.069

PRSS21 0.003 -1.825 0.000 -2.928 0.000 -2.466

PRTFDC1 0.030 -0.645 0.025 -0.391 0.000 3.680

PRTN3 0.000 -4.845 0.000 -6.827 0.000 -6.785

PSAP 0.019 -1.035 0.000 -2.560 0.000 -1.611

PSD3 0.011 0.335 0.000 6.187 0.644 0.036

PSTPIP1 0.000 -1.867 0.000 -3.390 0.000 -2.451

PSTPIP2 0.000 -2.447 0.000 -3.441 0.000 -3.068

PTAFR 0.000 -2.317 0.000 -3.775 0.000 -3.523

PTGER2 0.000 -1.548 0.000 -1.704 0.000 -2.186

170

PTGES 0.622 -0.226 0.000 -2.178 0.000 -1.720

PTGR1 0.000 -2.201 0.009 -0.600 0.000 -2.582

PTGS1 0.000 -2.465 0.000 -2.102 0.000 -2.861

PTGS2 0.000 -2.847 0.000 -4.430 0.000 -3.808

PTH2R 0.000 -2.104 0.000 -2.039 0.000 -1.978

PTK2 0.329 0.113 0.000 1.138 0.000 -2.728

PTP4A3 0.000 1.017 0.000 3.095 0.000 1.503

PTPN12 0.001 -1.082 0.000 -0.970 0.000 -2.329

PTPN14 0.483 0.090 0.000 2.857 0.000 2.933

PTPN18 0.348 -0.290 0.000 -0.457 0.000 -2.320

PTPN22 0.000 -2.105 0.000 -2.873 0.000 -1.095

PTPN7 0.025 -0.554 0.000 -2.654 0.004 0.319

PTPRCAP 0.000 2.071 0.000 2.272 0.000 2.108

PTPRD 0.008 0.321 0.000 1.467 0.000 3.380

PTPRJ 0.237 -0.698 0.000 -3.104 0.000 -3.859

PTX3 0.000 -3.600 0.000 -5.415 0.000 -4.984

PXDN 0.734 0.136 0.000 6.336 0.000 3.032

PXK 0.016 -0.605 0.000 0.542 0.000 -2.007

PXN 0.000 -1.336 0.000 -2.858 0.000 -2.773

PXYLP1 0.156 -0.800 0.000 -2.234 0.000 2.429

PYGL 0.000 -3.024 0.000 -4.595 0.000 -4.183

QKI 0.000 -1.578 0.000 -0.982 0.000 -2.867

QPCT 0.000 -3.171 0.000 -5.415 0.000 -4.922

QRSL1 0.000 1.746 0.000 2.573 0.000 0.462

QSOX1 0.000 -2.055 0.000 -3.134 0.000 -2.173

171

RAB11FIP1 0.000 -1.696 0.000 -2.985 0.000 -1.921

RAB13 0.000 -2.728 0.000 -0.898 0.000 -3.092

RAB15 0.001 0.429 0.000 2.057 0.000 2.521

RAB20 0.255 -0.674 0.000 -2.103 0.000 -2.452

RAB27A 0.000 -2.033 0.000 -3.450 0.000 -3.357

RAB27B 0.000 -2.380 0.000 -3.136 0.010 -0.863

RAB30 0.000 2.483 0.038 0.188 0.053 -0.326

RAB31 0.000 -2.565 0.000 -5.804 0.000 -4.976

RAB32 0.000 -2.748 0.000 -5.846 0.000 -1.745

RAB33A 0.000 -1.398 0.000 -2.746 0.713 0.103

RAB39B 0.000 0.985 0.012 0.353 0.000 2.011

RAB3D 0.000 -1.832 0.000 -2.838 0.000 -1.523

RABEP2 0.000 0.942 0.000 2.933 0.000 1.669

RAC2 0.000 -1.807 0.000 -2.143 0.000 -1.546

RAG1 0.581 0.394 0.000 4.848 0.000 1.903

RAG2 0.015 0.900 0.000 2.903 0.000 1.598

RAPGEF5 0.000 1.789 0.000 1.768 0.000 2.718

RASD1 0.757 -0.145 0.000 3.995 0.440 0.190

RASEF 0.038 1.126 0.000 1.906 0.000 2.279

RASGEF1A 0.114 -0.782 0.000 -0.408 0.000 -2.342

RASSF2 0.224 -0.431 0.000 -1.155 0.000 -2.595

RASSF3 0.168 -0.360 0.002 -0.358 0.000 -2.315

RASSF4 0.001 -2.135 0.000 -1.287 0.000 -2.752

RASSF6 0.000 3.555 0.565 0.057 0.000 0.918

RBM15B 0.000 0.860 0.000 1.420 0.000 2.203

172

RBM47 0.012 -1.143 0.000 -2.325 0.000 -3.433

RBP7 0.000 -3.001 0.000 -5.365 0.000 -4.495

RCAN1 0.025 -0.756 0.000 2.915 0.009 0.348

RCBTB2 0.475 -0.443 0.000 2.004 0.000 2.966

REEP5 0.013 -0.722 0.000 -1.087 0.000 -2.659

REPS2 0.000 -2.505 0.000 -3.190 0.000 -2.994

RETN 0.000 -2.703 0.000 -5.016 0.000 -4.265

RFESD 0.000 -3.152 0.000 -3.341 0.000 -3.146

RFLNB 0.000 -2.854 0.000 -2.107 0.000 -1.991

RFTN1 0.000 1.241 0.000 2.326 0.000 0.725

RFX3 0.012 0.279 0.000 1.218 0.000 2.077

RFX7 0.027 0.763 0.000 1.199 0.000 2.100

RGCC 0.000 -1.850 0.000 -2.794 0.000 -2.300

RGL1 0.525 -0.245 0.000 3.840 0.000 -0.457

RGL4 0.000 -3.192 0.000 -4.731 0.000 -3.154

RGS13 0.000 2.092 0.295 0.202 0.468 0.144

RGS16 0.000 3.496 0.000 1.097 0.000 -0.705

RGS18 0.000 -4.051 0.000 -4.469 0.000 -3.771

RGS2 0.624 -0.231 0.000 -3.212 0.000 -2.956

RHAG 0.000 -4.728 0.000 -3.674 0.000 -3.487

RHD 0.000 -3.744 0.000 -3.863 0.000 -4.551

RHOB 0.676 0.333 0.013 0.678 0.000 -2.336

RHOH 0.011 0.999 0.000 1.499 0.000 2.671

RHOU 0.000 -2.106 0.000 -2.954 0.514 -0.233

RIMKLB 0.054 0.396 0.000 2.361 0.000 0.963

173

RIN2 0.013 -1.189 0.000 -1.125 0.000 -2.710

RIOK3 0.001 -1.443 0.000 -2.163 0.000 -2.488

RIPK2 0.568 0.435 0.002 -0.628 0.000 -2.755

RNASE2 0.000 -2.903 0.000 -5.696 0.000 -4.854

RNASE3 0.000 -4.369 0.000 -6.974 0.000 -6.307

RNASE6 0.003 -1.582 0.000 -2.057 0.000 -2.220

RNF144A 0.000 -2.018 0.000 -1.658 0.233 0.015

RNF144B 0.000 1.211 0.000 -3.495 0.000 -2.887

RNF150 0.162 0.135 0.312 -0.035 0.000 2.456

RNF182 0.000 -2.711 0.000 -2.990 0.000 -2.901

RNF217 0.000 -2.079 0.000 -2.503 0.000 -2.625

RNF24 0.000 -2.695 0.000 -1.307 0.000 -1.085

RNFT2 0.000 2.454 0.000 0.995 0.000 1.102

ROGDI 0.000 -1.800 0.000 -1.329 0.000 -2.880

ROPN1L 0.000 -2.401 0.000 -2.830 0.000 -2.961

RORA 0.008 -1.704 0.000 -3.269 0.026 -0.672

RORB 0.470 0.071 0.020 0.483 0.000 2.281

RPS29 0.000 0.557 0.000 1.919 0.000 2.381

RPS6KA2 0.000 -2.023 0.000 -1.569 0.000 -2.609

RRAGD 0.000 -2.641 0.000 -2.257 0.000 -1.932

RTN1 0.008 -1.495 0.000 -2.328 0.000 -1.946

RTN3 0.000 -1.530 0.000 -2.130 0.000 -1.708

RTN4 0.000 -0.710 0.000 -0.498 0.000 2.067

RUBCNL 0.000 3.557 0.003 0.975 0.372 -0.327

RUFY3 0.000 0.948 0.000 1.851 0.000 3.587

174

RUNX1-IT1 0.497 -0.464 0.000 1.472 0.000 2.014

RUNX2 0.000 -1.679 0.000 -3.171 0.000 -1.678

RUNX3 0.278 0.185 0.000 -2.281 0.000 -1.244

RXRA 0.000 -2.019 0.000 -3.037 0.000 -2.861

S100A10 0.022 -1.319 0.000 -2.501 0.000 -1.517

S100A11 0.005 -1.311 0.000 -4.903 0.000 -3.281

S100A12 0.000 -2.774 0.000 -5.606 0.000 -4.561

S100A13 0.000 1.432 0.000 3.733 0.002 -0.297

S100A16 0.526 -0.157 0.000 4.611 0.000 -0.379

S100A4 0.006 -0.858 0.000 -2.090 0.000 -2.067

S100A6 0.000 -1.304 0.000 -2.875 0.000 -2.596

S100A8 0.000 -0.855 0.000 -2.408 0.000 -1.904

S100A9 0.000 -1.599 0.000 -4.048 0.000 -3.233

S100P 0.000 -3.453 0.000 -6.276 0.000 -5.403

SALL2 0.001 0.482 0.000 0.700 0.000 2.541

SAMHD1 0.009 -1.313 0.000 -2.232 0.000 -2.460

SAMSN1 0.007 -1.524 0.000 -2.987 0.000 -1.969

SBF1 0.000 1.038 0.000 1.945 0.000 2.037

SBK1 0.071 0.620 0.388 0.127 0.000 3.202

SCAI 0.495 0.105 0.000 2.184 0.000 3.957

SCARB1 0.000 1.320 0.000 2.734 0.000 0.475

SCD 0.056 -0.749 0.000 -3.839 0.000 1.055

SCD5 0.008 0.525 0.000 2.538 0.000 3.605

SCGB3A1 0.861 -0.040 0.000 -0.292 0.000 2.974

SCN2A 0.000 0.091 0.020 0.328 0.000 2.706

175

SCN3A 0.021 0.028 0.000 2.769 0.000 3.373

SCO2 0.991 0.016 0.000 -2.411 0.000 -2.202

SCPEP1 0.000 -1.128 0.000 -1.820 0.000 -3.554

SCRN1 0.735 0.139 0.000 -2.255 0.000 -1.542

SDC2 0.725 0.055 0.000 2.456 0.000 -0.964

SDC4 0.222 -0.756 0.000 -1.950 0.000 -2.165

SDCBP 0.009 -0.678 0.000 -2.316 0.000 -1.505

SDK2 0.021 0.248 0.000 4.145 0.037 0.687

SEC11C 0.000 0.518 0.000 -2.203 0.000 -0.959

SEC14L4 0.000 -3.235 0.000 -3.538 0.000 -3.641

SEC31B 0.858 -0.081 0.000 1.294 0.000 2.822

SECTM1 0.211 0.739 0.000 -2.088 0.000 -1.635

SEL1L3 0.000 2.167 0.003 -0.871 0.000 -2.729

SELENBP1 0.000 -4.917 0.000 -4.802 0.000 -5.160

SELENOP 0.000 -4.092 0.000 -3.524 0.000 -4.854

SELENOW 0.562 -0.265 0.806 0.047 0.000 2.273

SELL 0.006 -1.479 0.000 -2.568 0.003 -0.752

SELP 0.001 -1.462 0.000 -2.121 0.000 -1.949

SELPLG 0.293 -0.432 0.000 -3.360 0.000 -0.809

SEMA4A 0.000 -1.827 0.000 -3.046 0.000 -2.848

SEMA6A 0.193 -0.328 0.000 2.779 0.000 -0.369

1-Sep 0.000 1.165 0.177 0.233 0.000 2.391

6-Sep 0.017 0.769 0.000 2.481 0.000 3.519

SERPINA1 0.047 0.327 0.000 -4.928 0.000 -3.538

SERPINB1 0.000 -2.378 0.000 -2.601 0.000 -2.072

176

SERPINB10 0.000 -3.840 0.000 -5.062 0.000 -4.854

SERPINB2 0.039 -1.619 0.000 -4.956 0.000 -3.921

SERPINB6 0.000 -2.020 0.000 -1.011 0.000 -3.300

SERPINB8 0.000 -0.941 0.000 -0.983 0.000 -2.004

SERPINE2 0.000 -1.959 0.000 -2.460 0.000 -1.359

SESN3 0.002 -1.858 0.000 -2.335 0.000 -3.313

SETD7 0.000 1.220 0.000 2.205 0.000 1.122

SGCE 0.000 3.161 0.000 1.386 0.601 -0.044

SGK1 0.004 -1.265 0.000 -1.848 0.000 -2.310

SGK223 0.619 0.420 0.000 2.802 0.006 -0.732

SGMS1 0.000 -1.360 0.000 -2.550 0.000 -2.554

SGMS2 0.000 -2.146 0.000 -3.500 0.000 -3.145

SH2B2 0.000 2.316 0.000 0.752 0.109 0.202

SH2D1A 0.003 0.441 0.000 -1.357 0.000 4.875

SH2D4B 0.016 0.206 0.000 2.268 0.631 0.010

SH3RF1 0.087 -0.438 0.000 2.848 0.000 0.701

SH3TC1 0.001 1.423 0.000 2.899 0.000 1.222

SHANK3 0.368 -0.138 0.000 4.799 0.000 1.562

SHISA2 0.002 0.832 0.000 2.437 0.000 4.151

SHTN1 0.001 -1.293 0.000 -3.018 0.000 -2.283

SIGLEC10 0.031 1.132 0.000 -2.068 0.000 -1.534

SIGLEC5 0.000 -2.302 0.000 -4.238 0.000 -3.756

SIGLEC7 0.026 -1.014 0.000 -2.792 0.000 -2.291

SIRPA 0.000 -2.212 0.000 -3.492 0.000 -3.292

SIRPB1 0.000 -1.770 0.000 -4.976 0.000 -4.203

177

SIRPB2 0.022 -1.110 0.000 -1.951 0.000 -2.058

SIT1 0.000 2.811 0.494 0.133 0.000 2.010

SKAP2 0.000 -1.769 0.000 -2.983 0.000 -3.601

SLA 0.001 -1.248 0.000 -3.296 0.000 -1.418

SLAMF7 0.066 0.941 0.000 -2.539 0.000 -2.618

SLC11A1 0.001 0.533 0.000 -3.852 0.000 -3.097

SLC14A1 0.000 -3.261 0.000 -2.841 0.000 -2.065

SLC15A3 0.139 -0.718 0.000 -3.005 0.000 -3.047

SLC16A14 0.002 -1.829 0.030 -0.712 0.000 -2.164

SLC16A6 0.457 -0.558 0.000 -3.991 0.000 -2.844

SLC19A2 0.072 -0.890 0.000 -3.190 0.153 0.315

SLC22A15 0.000 -2.812 0.000 -5.738 0.000 -4.511

SLC22A16 0.000 -4.249 0.000 -1.086 0.000 -2.251

SLC22A4 0.000 -3.862 0.000 -4.934 0.000 -4.985

SLC23A1 0.000 0.957 0.000 3.049 0.000 2.130

SLC24A4 0.063 -1.000 0.000 -3.039 0.000 -2.386

SLC25A21 0.000 -2.897 0.000 -3.109 0.000 -3.068

SLC25A36 0.088 0.290 0.000 1.079 0.000 2.032

SLC25A37 0.000 -2.550 0.000 -3.657 0.000 -3.215

SLC25A39 0.004 -1.831 0.000 -2.163 0.000 -2.472

SLC26A8 0.000 -1.755 0.000 -2.339 0.000 -2.168

SLC27A2 0.000 -1.790 0.000 -2.866 0.000 -2.958

SLC27A3 0.012 -0.975 0.000 1.485 0.000 -2.281

SLC28A3 0.000 -3.079 0.000 -3.705 0.000 -3.593

SLC29A1 0.085 -0.964 0.000 -2.307 0.000 -1.545

178

SLC2A1 0.021 -1.172 0.000 -1.322 0.000 -2.317

SLC2A3 0.000 -2.772 0.000 -2.546 0.000 -3.083

SLC2A5 0.000 -1.916 0.000 -1.914 0.000 -4.657

SLC2A6 0.000 2.243 0.433 -0.137 0.877 0.039

SLC30A4 0.001 0.939 0.000 1.570 0.000 2.022

SLC31A1 0.000 -1.521 0.000 -1.832 0.000 -2.035

SLC31A2 0.001 -1.667 0.000 -3.139 0.000 -3.109

SLC35D1 0.001 1.011 0.000 2.378 0.000 1.760

SLC35E3 0.067 0.071 0.000 2.492 0.001 0.153

SLC36A1 0.000 -1.910 0.000 -3.159 0.000 -2.702

SLC36A4 0.000 -1.997 0.000 -2.902 0.000 -2.017

SLC38A1 0.000 1.409 0.000 2.228 0.000 1.162

SLC39A11 0.002 -1.085 0.000 -2.387 0.000 -2.249

SLC40A1 0.000 -3.618 0.000 -5.343 0.000 -2.345

SLC41A1 0.711 0.095 0.000 2.491 0.445 0.105

SLC43A1 0.000 -1.864 0.000 -2.002 0.000 -2.920

SLC44A5 0.011 0.085 0.017 0.489 0.000 2.472

SLC45A4 0.000 -0.980 0.000 -2.056 0.000 -0.703

SLC4A1 0.000 -4.112 0.000 -3.114 0.000 -3.404

SLC4A4 0.281 -0.202 0.000 -0.309 0.000 2.370

SLC4A7 0.009 0.191 0.000 1.735 0.000 2.609

SLC5A3 0.045 0.378 0.000 1.682 0.000 2.609

SLC6A6 0.442 -0.435 0.000 -2.283 0.000 -1.724

SLC6A8 0.000 -3.594 0.000 -3.576 0.000 -4.039

SLC7A3 0.000 1.493 0.000 1.508 0.000 2.314

179

SLC7A7 0.447 0.433 0.000 -3.104 0.000 -3.266

SLCO3A1 0.000 -1.279 0.000 -2.592 0.000 -2.101

SLCO4C1 0.000 -2.400 0.000 -3.557 0.000 -3.072

SLFN13 0.000 -1.075 0.029 -0.558 0.000 -2.417

SLIT1 0.005 0.532 0.015 0.072 0.000 2.740

SLPI 0.000 -3.180 0.000 -5.843 0.000 -5.522

SMAD1 0.000 0.851 0.000 2.396 0.000 0.877

SMAGP 0.828 -0.061 0.000 3.428 0.000 1.596

SMARCA4 0.000 2.156 0.000 2.383 0.000 2.257

SMARCD3 0.001 -1.027 0.000 -2.795 0.000 -1.934

SMIM1 0.000 -1.783 0.000 -2.690 0.000 -2.802

SMIM24 0.000 -2.900 0.288 0.242 0.000 1.607

SMIM3 0.000 -2.064 0.000 1.724 0.011 -0.474

SMIM5 0.000 -2.453 0.000 -1.851 0.000 -2.455

SMPDL3A 0.869 -0.002 0.000 -3.680 0.000 -2.875

SNCA 0.000 -5.110 0.000 -3.359 0.000 -3.496

SNTB1 0.002 -0.795 0.000 -2.816 0.000 -2.891

SNX10 0.283 -0.419 0.000 -4.172 0.000 -1.091

SNX18 0.023 -0.883 0.000 -1.806 0.000 -2.654

SNX9 0.529 -0.446 0.221 -0.407 0.000 -2.540

SOCS2 0.000 -1.271 0.000 4.669 0.010 0.243

SOCS2-AS1 0.338 -0.076 0.000 4.874 0.000 0.715

SOCS7 0.000 1.071 0.000 1.258 0.000 2.188

SORT1 0.000 -1.266 0.000 -2.844 0.000 -2.700

SOX4 0.008 1.333 0.000 3.561 0.000 4.060

180

SOX6 0.000 -3.471 0.000 -3.319 0.000 -3.370

SOX7 0.103 0.026 0.000 2.990 0.031 -0.122

SPAG1 0.000 -1.640 0.000 -2.918 0.000 -1.390

SPARC 0.000 -3.786 0.000 -2.038 0.000 -2.967

SPECC1 0.000 -2.430 0.000 -0.987 0.000 -2.123

SPG20 0.000 -3.097 0.000 -2.570 0.000 -2.958

SPI1 0.442 -0.339 0.000 -1.168 0.000 -2.059

SPIB 0.000 2.576 0.001 0.973 0.000 -1.241

SPIDR 0.002 -1.631 0.000 -4.192 0.000 -3.649

SPNS3 0.000 -2.230 0.000 -2.139 0.001 0.678

SPOPL 0.673 -0.241 0.000 -1.616 0.000 -2.480

SPPL2B 0.045 0.434 0.000 2.043 0.000 1.143

SPRY1 0.207 -0.738 0.000 2.433 0.000 2.747

SPTA1 0.000 -4.255 0.000 -2.059 0.000 -3.815

SPTAN1 0.000 1.172 0.000 2.075 0.000 2.266

SPTB 0.000 -1.796 0.000 -2.310 0.000 -2.417

SPTBN1 0.000 0.694 0.000 2.547 0.000 2.088

SQRDL 0.000 -1.991 0.000 -3.081 0.000 -2.010

SRGN 0.000 -2.408 0.000 -3.704 0.000 -3.811

SRXN1 0.005 -1.049 0.000 -3.094 0.000 -2.820

SSBP2 0.000 0.812 0.000 3.592 0.000 2.860

SSBP3 0.000 -1.433 0.000 -2.362 0.000 0.617

ST14 0.000 2.916 0.000 -0.468 0.000 -0.671

ST18 0.606 -0.010 0.007 -0.026 0.000 2.826

STAG3 0.000 1.405 0.000 4.301 0.000 1.427

181

STAP1 0.000 2.059 0.000 2.118 0.000 -1.072

STAT3 0.000 -2.025 0.000 -2.122 0.000 -1.525

STAT4 0.131 -1.056 0.000 -2.606 0.038 -0.665

STEAP3 0.000 -3.134 0.000 -3.791 0.000 -3.845

STEAP4 0.007 -1.828 0.000 -3.083 0.000 -2.720

STK32B 0.626 -0.079 0.000 5.152 0.109 0.201

STK39 0.090 1.021 0.000 2.260 0.000 1.195

STMN3 0.440 -0.419 0.000 1.797 0.000 2.974

STOM 0.000 -2.999 0.000 -3.802 0.000 -4.740

STOX2 0.000 -1.830 0.000 -2.112 0.000 -1.328

STRADB 0.000 -2.809 0.000 -2.489 0.000 -2.681

STRBP 0.000 1.671 0.000 3.274 0.000 1.154

STX11 0.000 -2.220 0.000 -4.301 0.000 -3.996

STX3 0.000 -2.772 0.000 -1.309 0.000 -4.062

STXBP5 0.000 -1.632 0.000 -3.549 0.000 -1.790

SUCNR1 0.000 -2.544 0.000 -3.401 0.000 -2.778

SULF2 0.001 -1.580 0.000 -1.548 0.000 -2.961

SUSD3 0.024 0.054 0.000 -1.039 0.000 -2.529

SVIL 0.000 -2.377 0.000 -1.374 0.000 -1.351

SVIP 0.000 -2.616 0.000 -1.425 0.000 -1.059

SYNE1 0.000 -2.873 0.000 -4.742 0.000 -4.079

SYNGR1 0.000 -2.951 0.000 1.064 0.000 -3.444

TACSTD2 0.000 -3.666 0.000 -6.058 0.000 -5.566

TAGAP 0.003 -1.026 0.000 -1.931 0.000 -2.182

TAL1 0.000 -4.097 0.000 -4.842 0.000 -1.613

182

TANC1 0.028 0.201 0.001 0.698 0.000 2.763

TANC2 0.000 -1.621 0.000 -2.473 0.000 -0.732

TANGO2 0.000 -1.415 0.000 -1.509 0.000 -2.033

TANK 0.000 -1.151 0.000 -2.575 0.000 -1.567

TAPT1-AS1 0.013 0.806 0.000 2.734 0.000 0.826

TASP1 0.175 0.145 0.000 0.708 0.000 2.947

TBC1D16 0.196 0.066 0.000 2.775 0.000 1.001

TBC1D31 0.643 -0.042 0.000 0.691 0.000 2.169

TBC1D8 0.123 -0.689 0.000 -1.963 0.000 -2.422

TCEAL9 0.030 -0.847 0.000 -2.594 0.249 0.407

TCF3 0.000 1.325 0.000 2.466 0.000 1.458

TCF4 0.000 2.296 0.000 4.162 0.010 -0.503

TCF7 0.167 -0.369 0.006 -0.324 0.000 2.842

TCL1A 0.000 4.151 0.000 5.057 0.000 -2.040

TCN1 0.000 -4.343 0.000 -6.718 0.000 -6.266

TERF2 0.055 0.420 0.000 2.203 0.012 0.163

TERT 0.000 2.013 0.000 1.546 0.000 0.729

TES 0.697 0.188 0.000 -2.935 0.000 -1.712

TESC 0.000 -2.628 0.000 -4.209 0.000 -2.595

TESPA1 0.032 0.429 0.000 -0.402 0.000 2.109

TET2 0.004 -1.090 0.000 -2.088 0.000 -2.755

TFDP1 0.000 -1.235 0.000 -2.754 0.000 -2.466

TFDP2 0.005 0.686 0.000 -1.711 0.000 2.447

TFEB 0.001 0.702 0.000 1.306 0.000 -2.189

TFEC 0.000 -2.180 0.000 -3.829 0.000 -2.926

183

TFF3 0.000 -3.052 0.000 -3.373 0.000 -3.224

TFR2 0.000 -2.441 0.000 -1.911 0.000 -3.575

TFRC 0.000 -1.673 0.000 -2.424 0.000 -1.254

TGFBI 0.000 -1.687 0.000 -5.287 0.000 -4.271

TGFBR1 0.006 -1.176 0.000 -0.367 0.000 -2.193

TGFBR3 0.290 -0.704 0.000 -1.821 0.000 -2.404

THBD 0.286 -0.529 0.000 -2.699 0.000 -2.384

THBS1 0.157 0.524 0.000 -3.273 0.000 -3.628

THBS4 0.000 -1.700 0.000 -2.273 0.000 -2.433

THEM4 0.037 0.599 0.000 -2.457 0.000 -1.952

THEMIS2 0.001 -1.543 0.000 -1.123 0.000 -3.768

TIMD4 0.026 -1.736 0.000 -1.963 0.000 -2.017

TIMP2 0.003 -1.306 0.000 -2.566 0.000 -1.757

TKT 0.000 -1.159 0.000 -2.705 0.000 -2.610

TLE3 0.731 -0.324 0.000 -2.171 0.003 -0.526

TLR1 0.146 -0.852 0.000 -1.323 0.000 -3.864

TLR10 0.000 2.041 0.000 0.868 0.000 -0.409

TLR2 0.006 -1.228 0.000 -2.497 0.000 -3.570

TLR4 0.000 -1.570 0.000 -2.841 0.000 -2.617

TLR8 0.157 -1.010 0.000 -4.476 0.000 -3.661

TM6SF1 0.000 -2.815 0.000 -4.318 0.000 -3.596

TMBIM6 0.000 -1.562 0.000 -2.219 0.000 -2.202

TMCC2 0.001 -2.555 0.000 -2.590 0.000 -3.063

TMCC3 0.175 -0.905 0.000 -2.452 0.000 -2.541

TMED6 0.029 0.204 0.000 3.413 0.248 0.054

184

TMEM106A 0.559 -0.292 0.000 3.153 0.000 1.502

TMEM120A 0.023 -0.758 0.000 -1.500 0.000 -2.228

TMEM154 0.002 -1.072 0.000 -2.471 0.000 -1.916

TMEM156 0.000 0.657 0.000 2.154 0.000 0.761

TMEM158 0.000 -2.109 0.000 -2.127 0.000 -1.896

TMEM170B 0.000 -2.396 0.000 -3.848 0.000 -1.934

TMEM173 0.000 -2.062 0.000 -1.259 0.107 -0.301

TMEM176B 0.459 0.038 0.000 -2.738 0.000 -1.768

TMEM216 0.001 -0.996 0.000 -3.232 0.000 -2.413

TMEM237 0.000 0.963 0.000 0.580 0.000 2.530

TMEM263 0.011 1.085 0.000 3.405 0.000 2.457

TMEM268 0.000 1.815 0.000 2.794 0.000 1.277

TMEM30A 0.000 -1.845 0.000 -1.778 0.000 -2.123

TMEM52B 0.000 -2.140 0.000 -2.599 0.000 -2.451

TMEM55A 0.000 -2.942 0.000 -3.426 0.000 -3.268

TMEM56 0.000 -3.952 0.000 -4.863 0.000 -4.772

TMEM64 0.000 0.812 0.175 0.211 0.000 -2.126

TMEM71 0.000 -2.577 0.000 -3.217 0.000 -2.225

TMEM9B 0.000 -1.199 0.000 -1.793 0.000 -2.221

TMOD1 0.000 -2.918 0.000 -2.777 0.000 -3.540

TNFAIP2 0.034 -0.896 0.000 -2.228 0.001 -0.673

TNFAIP6 0.002 -2.076 0.000 -3.799 0.000 -3.251

TNFAIP8 0.064 -0.802 0.000 -2.082 0.000 -1.016

TNFRSF10C 0.000 -2.061 0.000 -3.832 0.000 -3.403

TNFRSF1B 0.140 -0.742 0.000 -2.119 0.000 -3.173

185

TNFSF13 0.000 -1.754 0.000 -2.506 0.000 -3.398

TNFSF13B 0.000 -2.078 0.000 -3.691 0.000 -4.122

TNFSF4 0.288 -0.377 0.000 3.795 0.000 1.288

TNS1 0.000 -5.065 0.000 -2.412 0.000 -4.793

TOX 0.112 0.670 0.000 1.505 0.000 4.386

TPM1 0.000 -2.403 0.000 -2.934 0.000 -2.984

TPM2 0.004 0.679 0.000 2.711 0.000 1.941

TPM4 0.027 0.251 0.000 2.138 0.000 1.473

TPP1 0.000 -1.136 0.000 -2.382 0.000 -2.039

TPRG1L 0.000 -1.411 0.000 -1.414 0.000 -2.081

TRAF3IP2 0.071 0.057 0.000 2.946 0.000 0.973

TRAF5 0.000 2.143 0.000 2.222 0.000 3.070

TRAK2 0.000 -2.605 0.000 -2.041 0.000 -2.699

TRAT1 0.552 -0.485 0.000 -1.638 0.000 3.578

TRBC1 0.708 0.342 0.000 -1.940 0.000 2.712

TREM1 0.010 -1.683 0.000 -5.015 0.000 -3.690

TRG-AS1 0.246 -0.453 0.000 0.653 0.000 2.675

TRIB1 0.005 -1.164 0.000 -3.175 0.000 -3.974

TRIB3 0.739 0.160 0.000 -3.121 0.000 -1.185

TRIM10 0.000 -4.714 0.000 -4.645 0.000 -4.643

TRIM58 0.000 -3.686 0.000 -1.887 0.000 -3.519

TRIO 0.019 0.246 0.000 2.011 0.000 -0.503

TRIQK 0.000 -2.570 0.000 -2.936 0.000 -1.928

TRMT6 0.088 0.361 0.000 -2.044 0.000 -1.541

TRO 0.001 0.683 0.000 0.508 0.000 2.014

186

TSHR 0.009 0.507 0.231 0.077 0.000 2.954

TSPAN2 0.000 -2.650 0.000 -3.015 0.000 -1.641

TSPAN32 0.000 -1.886 0.000 -2.136 0.000 -1.915

TSPAN5 0.000 -3.002 0.000 -2.105 0.000 -1.344

TSPAN7 0.239 -0.466 0.000 3.316 0.000 5.770

TSPO 0.000 -2.272 0.000 -3.127 0.000 -3.333

TSPO2 0.000 -3.825 0.000 -3.743 0.000 -3.948

TST 0.000 -1.583 0.000 -1.093 0.000 -2.936

TTC7A 0.000 0.796 0.000 2.101 0.000 1.191

TTC7B 0.000 -2.412 0.000 -1.023 0.000 -2.292

TTC8 0.000 -1.920 0.000 -3.501 0.000 -2.182

TUBA4A 0.000 -1.015 0.000 -1.499 0.000 -2.596

TUBB1 0.000 -5.474 0.000 -5.742 0.000 -5.822

TUBB2A 0.684 0.365 0.000 -2.145 0.432 -0.279

TUBB6 0.964 -0.042 0.000 -1.976 0.000 -3.263

TUBG1 0.008 -1.123 0.000 -2.013 0.000 -1.808

TUSC1 0.000 -1.742 0.001 -1.152 0.000 -3.013

TUSC3 0.078 0.145 0.001 0.733 0.000 2.323

TYMP 0.162 -0.659 0.000 -2.282 0.000 -2.551

TYROBP 0.000 -1.313 0.000 -4.730 0.000 -3.738

UAP1 0.265 -0.461 0.000 -2.018 0.000 -1.180

UBAC1 0.000 -1.424 0.000 -2.202 0.000 -2.115

UBASH3A 0.396 -0.471 0.000 -1.185 0.000 2.328

UBASH3B 0.107 -0.424 0.000 3.402 0.000 1.601

UBE2D1 0.252 -0.511 0.000 -1.844 0.000 -2.248

187

UBXN10-AS1 0.009 0.211 0.000 2.020 0.000 1.270

UBXN2B 0.259 -0.359 0.000 -2.459 0.000 -0.932

UCHL1 0.000 4.284 0.398 0.094 0.368 -0.011

UGCG 0.000 -2.374 0.000 -2.651 0.000 -3.562

UGGT2 0.000 0.837 0.000 -2.510 0.000 -2.291

UHRF1 0.847 0.117 0.000 2.654 0.000 1.422

UMODL1 0.000 0.833 0.000 1.453 0.000 3.289

UPP1 0.282 -0.597 0.000 -3.237 0.000 -2.664

UROD 0.000 -2.228 0.000 -2.135 0.000 -2.011

USP11 0.000 1.435 0.000 2.494 0.000 2.074

USP13 0.000 1.421 0.000 2.239 0.000 2.063

VAMP3 0.000 -1.820 0.000 -2.115 0.000 -2.118

VANGL1 0.098 -0.315 0.000 -0.698 0.000 2.090

VCAM1 0.000 -2.733 0.000 -3.052 0.000 -3.362

VCAN 0.002 -2.011 0.000 -4.867 0.000 -3.762

VCL 0.000 -2.181 0.000 -1.545 0.000 -2.726

VDR 0.019 -0.801 0.000 -2.620 0.000 -2.564

VEPH1 0.001 -1.272 0.000 -2.042 0.000 -1.822

VIM 0.000 -2.549 0.000 -0.926 0.000 -1.318

VNN1 0.010 -2.083 0.000 -2.859 0.000 -4.354

VNN2 0.290 -0.601 0.000 -3.466 0.000 -3.415

VNN3 0.001 -2.013 0.000 -3.958 0.000 -3.558

VPREB1 1.000 0.011 0.000 5.524 0.012 0.792

VPREB3 0.007 1.957 0.000 5.029 0.000 -1.849

VPS37C 0.000 -1.075 0.000 -2.167 0.000 -2.635

188

VPS8 0.001 -0.802 0.000 -2.050 0.000 -1.720

VSTM1 0.000 -3.258 0.000 -5.360 0.000 -4.509

WARS 0.113 0.476 0.000 -2.927 0.000 -2.210

WASF1 0.000 1.750 0.000 2.827 0.000 0.860

WASIR2 0.003 0.325 0.000 2.431 0.000 1.190

WDFY3 0.000 -1.840 0.000 -1.227 0.000 -2.458

WFS1 0.000 0.858 0.000 3.843 0.062 0.195

WIPI1 0.000 -2.332 0.000 -3.798 0.000 -3.919

WLS 0.000 -1.733 0.000 -2.023 0.000 -2.419

WT1 0.825 0.047 0.000 2.267 0.000 2.415

XCL1 0.017 -1.247 0.000 -3.287 0.000 -2.357

XK 0.000 -3.864 0.000 -4.421 0.000 -4.707

YME1L1 0.056 0.092 0.000 -2.044 0.000 3.061

YPEL1 0.001 0.929 0.000 2.679 0.000 2.450

YPEL4 0.000 -2.755 0.000 -2.965 0.000 -2.713

ZAP70 0.494 0.440 0.000 0.742 0.000 3.342

ZBED3 0.004 0.550 0.000 1.372 0.000 2.438

ZBP1 0.749 0.266 0.000 -2.188 0.000 -2.190

ZBTB10 0.001 0.363 0.000 2.180 0.000 1.358

ZBTB20 0.252 -0.337 0.000 1.115 0.000 2.026

ZBTB38 0.033 0.336 0.000 -2.518 0.000 -2.518

ZBTB8A 0.448 -0.247 0.000 1.996 0.000 4.013

ZC3H12D 0.053 0.944 0.000 3.211 0.000 -1.492

ZC3HAV1L 0.771 -0.214 0.000 1.390 0.000 2.060

ZC4H2 0.000 0.817 0.000 2.029 0.000 1.895

189

ZCCHC7 0.002 1.433 0.000 4.452 0.000 0.965

ZDHHC2 0.000 -1.700 0.000 -1.361 0.000 -2.256

ZDHHC3 0.000 -1.469 0.000 -2.427 0.000 -2.090

ZEB1 0.266 0.043 0.089 0.398 0.000 2.108

ZEB2 0.149 -0.717 0.000 0.950 0.000 -2.170

ZFP1 0.006 0.912 0.000 1.122 0.000 2.311

ZFPM1 0.016 -0.730 0.000 -2.063 0.000 -0.740

ZG16B 0.000 -1.647 0.000 -2.906 0.000 -2.493

ZHX2 0.000 1.520 0.000 2.856 0.000 0.613

ZMAT3 0.001 1.077 0.000 2.724 0.000 2.583

ZMYM6 0.000 -2.200 0.000 -1.024 0.000 -1.869

ZNF107 0.000 0.399 0.000 2.560 0.000 1.995

ZNF117 0.000 0.700 0.000 2.713 0.000 1.270

ZNF185 0.000 -2.251 0.000 -1.888 0.000 -2.220

ZNF385B 0.000 3.821 0.624 0.028 0.601 0.055

ZNF423 0.995 0.004 0.000 5.752 0.000 3.035

ZNF444 0.000 1.467 0.000 1.687 0.000 2.208

ZNF467 0.000 -1.387 0.000 -1.610 0.000 -2.442

ZNF496 0.000 0.302 0.000 1.997 0.000 2.717

ZNF512B 0.058 0.641 0.000 1.287 0.000 2.486

ZNF516 0.010 -0.904 0.000 -1.585 0.000 -2.118

ZNF521 0.010 0.633 0.520 -0.150 0.000 2.331

ZNF529 0.089 0.665 0.000 1.540 0.000 2.366

ZNF57 0.001 1.012 0.000 2.849 0.000 3.265

ZNF608 0.000 2.277 0.000 4.122 0.024 0.319

190

ZNF618 0.508 -0.205 0.000 2.428 0.000 1.873

ZNF70 0.761 0.130 0.000 2.628 0.026 0.289

ZNF704 0.006 0.625 0.000 3.550 0.000 2.388

ZNF706 0.001 0.725 0.000 2.098 0.000 2.197

ZNF709 0.000 0.778 0.000 3.024 0.000 2.595

ZNF711 0.000 1.030 0.000 1.120 0.000 3.494

ZNF738 0.090 0.668 0.000 2.050 0.000 2.721

ZNF827 0.000 2.302 0.000 1.749 0.000 3.887

ZNF91 0.032 1.347 0.000 2.747 0.000 2.721

ZNRF1 0.000 -1.592 0.000 1.890 0.000 3.506

ZSCAN2 0.107 0.194 0.000 1.943 0.000 2.199

ZYX 0.000 -1.675 0.000 -2.143 0.000 -2.381

191

Appendix L

DEGs with greater than +/- 2-log-fold change

192

DEGs with greater than +/- 2-log-fold change among 3 leukemia subsets

B-ALL with t(8;14) Pooled B-ALL without t(8;14) T-ALL Gene Log FC Gene Log FC Gene Log FC PEG10 4.75864 CSRP2 6.735985 TSPAN7 5.76955 UCHL1 4.28351 PXDN 6.335653 HHIP-AS1 5.58375 TCL1A 4.15142 PSD3 6.187163 CHI3L2 5.38168 BLK 4.06095 ZNF423 5.752159 ARPP21 5.07244 IGF2BP3 3.84243 CD19 5.705484 HHIP 5.01194 ZNF385B 3.82051 EBF1 5.648731 ELOVL4 4.99132 FCRLA 3.79575 DNTT 5.610306 CTHRC1 4.92959 CD19 3.78277 VPREB1 5.523951 CD1E 4.89232 RUBCNL 3.55742 ECM1 5.376437 MLLT11 4.89110 RASSF6 3.55503 CTGF 5.232749 SH2D1A 4.87542 DTX1 3.54189 NPY 5.231316 CD1B 4.63886 MCOLN2 3.51433 STK32B 5.151912 LOC10050550 4.59039 RGS16 3.49551 TCL1A 5.057323 DNTT 4.53981 CNR1 3.48198 LINC01013 5.054765 MYO7B 4.53138 HRK 3.45732 LOC100130458 5.040505 NREP 4.48950 PCDH9 3.45064 VPREB3 5.028716 H2BFXP 4.41912 ID3 3.43057 MACROD2 4.919058 TOX 4.38638 CD79B 3.42517 BLNK 4.900857 CRNDE 4.33364 AEBP1 3.28449 SOCS2-AS1 4.87385 CASC15 4.33140 EML6 3.21289 RAG1 4.848365 IKZF2 4.22346 SGCE 3.16131 SHANK3 4.798806 KCTD1 4.16639 CD79A 3.14261 COL5A1 4.760098 PCAT18 4.16463 CLECL1 3.10672 SOCS2 4.668747 SHISA2 4.15140 BTNL9 3.07388 S100A16 4.610645 SOX4 4.05986 IFNLR1 3.06347 MYO5C 4.564428 CD3D 4.01542 BEST3 2.96850 MME 4.533874 ZBTB8A 4.01286 ST14 2.91634 JUP 4.503304 FAT1 3.99682 P2RX5 2.87488 HMHB1 4.462488 ADA 3.96605 PAX5 2.81894 ZCCHC7 4.451519 SCAI 3.95748 SIT1 2.81121 PAX5 4.394562 PEX5L 3.93545 CAMK2D 2.78722 NAV1 4.319883 CD3G 3.92579 CD72 2.78027 STAG3 4.30085 ZNF827 3.88720 FCRL2 2.75340 CYGB 4.275507 CDK6 3.84171 AMN 2.75173 ID3 4.225248 IRX5 3.80438 POU2AF1 2.73287 ARPP21 4.19672 MAP1A 3.79510 GPM6A 2.70094 CMTM8 4.191228 LCK 3.76119 IGHM 2.67222 TCF4 4.162106 LEF1 3.75993 PRDM15 2.66927 SDK2 4.144844 LDOC1L 3.72230 OSBPL10 2.66671 ZNF608 4.121751 PARD3 3.70592 E2F5 2.58554 DPEP1 4.062542 PRTFDC1 3.67955 BCAS4 2.58310 BLK 4.036841 AEBP1 3.67396 193

SPIB 2.57584 LEF1-AS1 4.021419 DBN1 3.66234 CD22 2.53516 RASD1 3.995099 GXYLT2 3.62175 LOC10012944 2.53296 CLIP2 3.949653 FXYD2 3.60626 BMP7 2.52630 CRIM1 3.941781 SCD5 3.60532 ELL3 2.51182 CD72 3.928398 RUFY3 3.58744 BEND4 2.50653 WFS1 3.84292 TRAT1 3.57772 RAB30 2.48269 RGL1 3.840368 CEP70 3.56610 RNFT2 2.45354 GPSM1 3.82878 MYEF2 3.52471 EBF1 2.44301 AEBP1 3.826969 IKZF2 2.43551 POU4F1 3.822557 ZNRF1 3.50592 DMD 2.39608 CD200 3.809214 ZNF711 3.49433 BLNK 2.37489 TNFSF4 3.794926 LOC10106039 3.46801 PAWR 2.36578 FAM69B 3.789159 LRP12 3.46585 CADM1 2.36372 AUTS2 3.782747 CD1A 3.45813 MTCL1 2.36331 CD22 3.776326 PTPRD 3.38007 SH2B2 2.31608 S100A13 3.733369 SCN3A 3.37320 ZNF827 2.30233 MEF2C 3.73182 CAMK4 3.34969 GJC1 2.29881 CD79A 3.729215 CACNB3 3.34808 TCF4 2.29573 CD79B 3.707795 MSI2 3.34689 ZNF608 2.27690 FLT3 3.699143 ZAP70 3.34150 MAPK12 2.24376 FAM129C 3.670972 LINC01260 3.30228 SLC2A6 2.24348 HLA-DOA 3.668849 UMODL1 3.28877 FADS3 2.24274 LEF1 3.648044 CLIP3 3.28119 PMAIP1 2.21393 CIITA 3.617458 BAHCC1 3.27230 FAM129C 2.20128 NT5E 3.613406 ZNF57 3.26492 MS4A1 2.19243 DBN1 3.610414 PIK3C2B 3.25393 MYC 2.17298 FADS3 3.600531 CD7 3.23965 OAS1 2.17142 SSBP2 3.592255 EPHB6 3.21250 SEL1L3 2.16738 SOX4 3.560516 SBK1 3.20169 SMARCA4 2.15619 ZNF704 3.549638 CHRNA3 3.17960 TRAF5 2.14281 AFF3 3.541537 FAM110C 3.16937 MARCKSL1 2.12399 KCNK12 3.520033 ABI2 3.16256 HLA-DOB 2.12085 BCL7A 3.490274 HES4 3.13982 HLA-DPA1 2.11647 CD34 3.482346 MZB1 3.13611 GPR18 2.10365 FAM150B 3.470401 PLCG1 3.10416 PPFIBP2 2.09970 NPR1 3.461976 GUCY1A3 3.08975 RGS13 2.09210 PAN3-AS1 3.436176 TRAF5 3.06962 LRMP 2.08942 SMAGP 3.428144 YME1L1 3.06084 PTPRCAP 2.07070 PLEKHG4B 3.418811 CDCA7 3.05756 BCL7A 2.06201 TMED6 3.413322 ZNF423 3.03519 STAP1 2.05890 CLEC14A 3.407185 PXDN 3.03236 PHGDH 2.04708 TMEM263 3.40546 LZTFL1 3.03031 TLR10 2.04080 UBASH3B 3.402108 LINC01215 3.02661 BACH2 2.03777 LOC285097 3.398495 GATA3 2.99659 FAM167A 2.03602 ENAH 3.385695 BCL11B 2.99595 194

PDLIM1 2.01407 PCDH9 3.369264 NRXN2 2.98345 TERT 2.01277 GNAI1 3.343503 STMN3 2.97388 ITPR3 2.00387 TSPAN7 3.315523 SCGB3A1 2.97386 NTNG2 -2.00045 PIK3C2B 3.296379 MEX3B 2.96618 KAT2B -2.006 GUCY1A3 3.292083 RCBTB2 2.96605 GCLM -2.00624 AHDC1 3.287957 TSHR 2.95410 IL1R1 -2.00674 STRBP 3.274388 KCNK17 2.95277 HAGH -2.0071 BCL11A 3.255449 TASP1 2.94732 FRY -2.00755 DCHS1 3.242336 GALNT2 2.94068 FCHO2 -2.00936 ZC3H12D 3.210683 PTPN14 2.93333 IGHD -2.01074 CTHRC1 3.200537 MAL 2.93257 FAM117A -2.01097 BANK1 3.195929 CDH2 2.92161 GAS2L3 -2.0112 MSI2 3.186135 CR2 2.91372 VCAN -2.01136 IGF2BP3 3.171736 PLCB4 2.91218 VNN3 -2.01323 POU2AF1 3.161422 CD3E 2.89862 NLRP12 -2.0144 CACNB3 3.161095 PGGHG 2.89077 POMGNT2 -2.0156 TMEM106A 3.153125 CYTH2 2.87819 ERLIN1 -2.01731 C5orf56 3.142862 LPAR6 2.87357 RNF144A -2.01758 LINC00865 3.133389 C11orf95 2.87022 RXRA -2.01857 NRN1 3.123953 HES1 2.86081 CCR2 -2.01913 LDLRAD4 3.118056 SSBP2 2.86040 NLRC4 -2.0198 CD109 3.114848 CCDC136 2.85211 SERPINB6 -2.01991 FHL1 3.114036 PLEKHA5 2.85116 ITGA9 -2.02045 PRKCE 3.113794 TCF7 2.84236 RPS6KA2 -2.02287 FMNL2 3.097222 LDLRAD4 2.83030 GPR160 -2.02389 PTP4A3 3.095465 ST18 2.82563 LYST -2.02454 LPAR6 3.090575 SEC31B 2.82220 STAT3 -2.02518 INO80C 3.059716 ARHGEF10 2.81763 LAMP2 -2.02787 SLC23A1 3.04928 LEF1-AS1 2.81339 GRB10 -2.03075 MLLT11 3.033469 LINC01550 2.81242 RAB27A -2.03302 MDK 3.030129 NINL 2.80753 ZNF709 3.023561 AHI1 2.79923 GYS1 -2.036 JADE3 3.023192 PRKRA 2.78627 CALD1 -2.03837 LINC01215 3.005535 NDST3 2.77532 PHACTR2 -2.03992 IRX1 3.001782 TANC1 2.76317 NR4A2 -2.04237 BCAS4 3.000458 SPRY1 2.74672 CYSTM1 -2.0475 LCN10 2.994578 SLIT1 2.74049 NATD1 -2.0497 SOX7 2.989512 CD96 2.73477 ALOX5AP -2.0541 MZB1 2.984603 CD99 2.73370 QSOX1 -2.05477 MAPKBP1 2.982322 PABPC4L 2.73048 FYB -2.0551 DDIT4L 2.981392 ZNF91 2.72146 EMB -2.05964 GAB1 2.977039 KIF3A 2.72113 TNFRSF10C -2.06136 ERG 2.964632 ZNF738 2.72099 TMEM173 -2.06249 CD9 2.959482 RAPGEF5 2.71845 SMIM3 -2.06351 ARHGEF10 2.958793 ZNF496 2.71684 195

AQP3 -2.06569 TRAF3IP2 2.945849 TRBC1 2.71229 ARHGAP18 -2.06649 BTNL9 2.945816 SCN2A 2.70587 FBN1 -2.06986 RABEP2 2.933209 DCHS1 2.70408 CA4 -2.07101 MTCL1 2.929811 LINC01120 2.70405 FOS -2.07483 APCDD1 2.926723 NAT14 2.69940 ARHGEF37 -2.07571 BAALC 2.917884 FGFR1 2.69338 TNFAIP6 -2.0762 RCAN1 2.914643 TRG-AS1 2.67464 FPR1 -2.07759 RAG2 2.903296 RHOH 2.67072 TNFSF13B -2.07777 ICOSLG 2.899452 PFKP 2.65965 KCNE3 -2.07784 SH3TC1 2.899039 ACVR2B 2.64654 RNF217 -2.07875 AKAP12 2.884306 DGKE 2.64443 CEBPA -2.08169 ERVH-3 2.871839 CEL 2.63637 DCAF12 -2.08231 H2BFXP 2.859247 CD109 2.63214 VNN1 -2.08311 PRCD 2.858646 DTX3 2.62780 HAL -2.08329 PTPN14 2.856919 LOC10013047 2.62141 CPEB4 -2.0893 ZHX2 2.85613 SLC4A7 2.60941 LXN -2.09339 ZNF57 2.849203 SLC5A3 2.60857 CAT -2.09353 SH3RF1 2.847867 IRX3 2.59882 MGST3 -2.09463 C10orf10 2.845377 B4GALT6 2.59855 JAK2 -2.09516 ADA 2.844164 ZNF709 2.59530 F5 -2.09536 WASF1 2.826589 CBFA2T3 2.59186 CACNA2D3 -2.0999 LOC100505501 2.814512 ZMAT3 2.58344 ACRBP -2.10159 DLL1 2.811132 GABPB1-AS1 2.58088 PTH2R -2.10438 BEND5 2.80828 PRKCQ 2.56418 PTPN22 -2.10471 HHIP-AS1 2.807473 PCNX2 2.56388 P2RY13 -2.10534 INSR 2.802655 ITM2A 2.55343 RHOU -2.10574 SGK223 2.802383 LOC285097 2.55247 CXCL1 -2.10871 TMEM268 2.793646 ADAMTS17 2.54206 TMEM158 -2.10871 HPS4 2.790444 SALL2 2.54102 IRAK3 -2.11259 C11orf95 2.783548 CEP128 2.53671 ABCC5 -2.11515 CBFA2T3 2.781019 TMEM237 2.53006 LINC01268 -2.11672 GBAT2 2.780725 MARCKSL1 2.52898 C1orf228 -2.11798 SEMA6A 2.778861 RAB15 2.52059 AMMECR1 -2.12249 TBC1D16 2.775476 CMAHP 2.51895 MYADM -2.12281 LOC100505564 2.773189 EVL 2.51225 CLEC4A -2.12662 SCN3A 2.769477 OCIAD2 2.49820 IL1RN -2.13387 PDLIM1 2.769019 NLGN4X 2.49495 RASSF4 -2.13537 LRP6 2.761604 MED17 2.48797 TMEM52B -2.13963 IGHM 2.760965 ZNF512B 2.48617 BMX -2.14083 ZNF91 2.746663 LRRC1 2.48324 PPP1R3D -2.14155 GABPB1-AS1 2.736433 CCR9 2.47969 B4GALT5 -2.14368 SCARB1 2.734333 FBXO41 2.47879 CD58 -2.14416 TAPT1-AS1 2.733835 NKX3-1 2.47296 SGMS2 -2.14588 CEP68 2.725005 SLC44A5 2.47167 HEBP1 -2.14763 ZMAT3 2.72356 MYL6B 2.47045 196

CLU -2.14842 FAM213A 2.719274 GALNT6 2.46438 FRMD4B -2.14896 LINC00642 2.718919 LOC10260646 2.4641 ALDH3B1 -2.14934 ZNF117 2.712951 HIVEP3 2.46138 KIF1B -2.15982 TPM2 2.711002 PHC1 2.45818 LOC10004971 -2.16709 MYRIP 2.708899 TMEM263 2.45672 NEURL1B -2.16857 GNG7 2.704788 RNF150 2.45583 IL18RAP -2.17065 HIST1H2BD 2.689275 OGN 2.45550 DNAJC6 -2.17085 BAHCC1 2.686313 NOTCH3 2.45431 ICAM4 -2.17536 YPEL1 2.679047 ARMCX2 2.45329 GATA1 -2.17576 PARD3 2.674649 YPEL1 2.45045 ELL2 -2.17578 LRRC8C 2.667786 PIK3R3 2.44962 TFEC -2.17981 ELFN2 2.66158 TFDP2 2.44719 VCL -2.18057 UHRF1 2.653546 ZBED3 2.43784 CSF3R -2.18115 LRMP 2.651898 PXYLP1 2.42876 DYSF -2.18495 AFDN 2.651085 CEP85L 2.42366 MSRA -2.18644 GBP4 2.633632 N4BP2 2.42259 GLT1D1 -2.19435 ZNF70 2.627755 LGALS3BP 2.42083 MSRB1 -2.1956 NRXN2 2.613015 P2RX5 2.41688 DUSP6 -2.19891 FAM69C 2.610662 PKIA 2.41514 ZMYM6 -2.19978 ARL10 2.598057 WT1 2.41484 PTGR1 -2.2012 C21orf2 2.589816 PCDH10 2.39673 CAPG -2.20252 PRKX 2.585461 NMT2 2.39441 NUCB2 -2.20376 QRSL1 2.572584 9/1/2017 2.39141 ABCB6 -2.20586 GPM6B 2.564447 ZNF704 2.38812 SIRPA -2.21207 HIST1H2AM 2.560876 RPS29 2.38116 FES -2.21297 ZNF107 2.559943 PHGDH 2.37471 DNAH10 -2.21487 SPTBN1 2.547026 SLC4A4 2.37026 CYTL1 -2.21593 LAMC1 2.540781 ZNF529 2.36554 ITGA2B -2.2168 SCD5 2.538461 ADGRG1 2.35909 LGALS12 -2.21803 ABHD17B 2.535559 MAST4 2.35212 C10orf11 -2.21852 BACH2 2.526888 HDGFRP3 2.34039 STX11 -2.22029 BMP2 2.526302 KCNA3 2.33768 FUT4 -2.22502 MOB3B 2.523431 CSRNP2 2.33418 UROD -2.22777 MYO1B 2.522954 CBX2 2.33270 LAIR1 -2.22931 COL27A1 2.510802 ZNF521 2.33087 SPNS3 -2.22992 GDF11 2.510679 UBASH3A 2.32800 PKLR -2.23433 HSPB1 2.50838 BCL7A 2.32612 HMBS -2.23521 KLF8 2.496594 ARHGAP32 2.32561 INPP1 -2.23586 USP11 2.493748 TUSC3 2.32345 APOC1 -2.23862 SLC35E3 2.492315 LINC00342 2.32332 FZD5 -2.24005 SLC41A1 2.490883 CARMIL2 2.31440 ALDH1A1 -2.24186 HMCES 2.487722 SLC7A3 2.31404 BPGM -2.24281 PLEKHG1 2.487508 GSPT2 2.31212 EPDR1 -2.24827 KLHL6 2.485137 ZFP1 2.31146 ZNF185 -2.25085 9/6/2017 2.481449 RORB 2.28096 197

NNMT -2.26019 LOC101928841 2.472588 RASEF 2.27917 INHBA -2.26078 DYRK2 2.468028 SELENOW 2.27330 ERG -2.26555 DDAH2 2.466435 SPTAN1 2.26633 KIAA1211L -2.26656 TCF3 2.465815 ELP2 2.26132 LOC10192842 -2.26741 PMAIP1 2.463433 AJAP1 2.26044 LRRC4 -2.26993 SDC2 2.456153 GNA15 2.26022 TSPO -2.27243 DIP2C 2.453312 SMARCA4 2.25707 IGF1R -2.2775 PPP1R16B 2.451973 BICD1 2.25012 NCEH1 -2.27834 ETV5 2.443253 FZD6 2.23385 ABHD5 -2.2849 NREP 2.440376 ANTXR1 2.23250 CLIC2 -2.2874 SHISA2 2.43714 ANKRD36B 2.22946 PDZD8 -2.29449 SPRY1 2.433444 NSG1 2.22672 ALAD -2.29854 CSNK1E 2.431912 DUXAP10 2.22542 CRISPLD2 -2.30157 WASIR2 2.43122 DNMT3A 2.22248 SIGLEC5 -2.30189 ZNF618 2.428136 LAT 2.21995 CITED2 -2.30348 IGHV5-78 2.427541 ZNF444 2.20774 CAPN3 -2.30527 PHLPP1 2.425282 RBM15B 2.20348 MCTP1 -2.31294 EGFL7 2.423909 MKL2 2.19934 CMTM2 -2.31423 ERO1B 2.414961 ZSCAN2 2.19918 CCND2 -2.31593 MYL6B 2.410453 ZNF706 2.19713 PTAFR -2.31667 CALN1 2.408787 GTF3A 2.19363 HK3 -2.31767 SMAD1 2.396324 GRK3 2.19273 CDKN2AIPN AGTRAP -2.31874 PPP3CC 2.39548 L 2.18888 ATPIF1 -2.32086 SMARCA4 2.382561 SOCS7 2.18759 CSTA -2.3235 LIMD1 2.382283 MAGED1 2.18140 CMBL -2.32549 SLC35D1 2.378214 PITPNM2 2.17955 LGALS3 -2.32549 MLXIP 2.367882 C5orf56 2.17910 IVNS1ABP -2.3315 PRDX1 2.362679 EIF3M 2.17470 LOC10192800 GCA -2.33177 ALDH5A1 2.361667 0 2.17125 WIPI1 -2.33183 CDK6 2.360569 TBC1D31 2.16851 AIG1 -2.33189 RIMKLB 2.360567 CIB2 2.16757 P2RY2 -2.3323 HSPC324 2.348161 DHX30 2.15717 DHRS9 -2.33659 CHST15 2.346228 NUDT5 2.13540 ATP2B4 -2.33706 PHC1 2.345327 SLC23A1 2.13011 ENTPD1 -2.34449 FLT4 2.335451 NPTX2 2.12846 MAP7 -2.35934 LGR5 2.331826 AFF3 2.12586 MICALL2 -2.36582 KHDRBS3 2.331016 ARHGAP29 2.12341 CSGALNACT 1 -2.37237 RFTN1 2.326275 GKAP1 2.12159 CD33 -2.37277 FLNB 2.32405 FLJ10038 2.10892 UGCG -2.3741 ERGIC1 2.318037 TESPA1 2.10882 CES1 -2.37589 ALDH7A1 2.317734 PTPRCAP 2.10831 SVIL -2.37714 ATP8B2 2.314063 ZEB1 2.10771

198

SERPINB1 -2.37839 GALNT7 2.308887 NUDT11 2.10597 C11orf74 -2.37852 ASAP2 2.308853 RFX7 2.10013 RAB27B -2.37973 LIG4 2.301312 VANGL1 2.09032 MPP7 -2.39336 ARHGEF7 2.297777 SPTBN1 2.08810 KRT1 -2.39377 GREM1 2.297406 JADE3 2.08757 FAM105A -2.39623 FBXO41 2.28976 DSG2 2.08605 TMEM170B -2.39635 GALNT2 2.2892 FAM169A 2.08186 DACH1 -2.39899 NUDT5 2.286061 NUCB2 2.07764 CDC42BPA -2.39952 GTF2IRD1 2.284871 RFX3 2.07686 SLCO4C1 -2.39977 CCDC50 2.283735 USP11 2.07438 ADGRG3 -2.40096 ADCY9 2.277683 LRP8 2.07113 ROPN1L -2.40128 PTPRCAP 2.271561 RTN4 2.06711 CEACAM21 -2.40198 HAR1A 2.268446 MEX3C 2.06650 TPM1 -2.40258 SH2D4B 2.267579 ILDR2 2.06589 APOBEC3B -2.40291 WT1 2.266865 DNMT3B 2.06282 SRGN -2.40849 KLHL24 2.265902 USP13 2.06273 TTC7B -2.41222 NEGR1 2.261367 ZC3HAV1L 2.05979 C11orf21 -2.41509 NR3C1 2.260494 HELLS 2.05504 NCF2 -2.41742 FPGS 2.259847 PMAIP1 2.04115 ERMAP -2.4224 STK39 2.259535 MYT1L 2.04111 SPECC1 -2.43002 ADGRA3 2.258416 SBF1 2.03700 CTTN -2.43478 ARHGAP29 2.257784 MGA 2.03463 TFR2 -2.44077 USP13 2.239158 ITPR2 2.03204 MBOAT1 -2.44221 ITGA6 2.238858 SLC25A36 2.03198 ANKRD22 -2.44349 P2RY14 2.238678 PCDH9 2.02804 PSTPIP2 -2.44699 ENG 2.237674 GAS2 2.02591 ANXA1 -2.44746 MXRA7 2.228 DLG5 2.02582 MANSC1 -2.44989 SLC38A1 2.227647 ZBTB20 2.02554 PRAM1 -2.45179 LOC101060391 2.225281 PLEKHG4B 2.02497 SMIM5 -2.45304 HACD1 2.223588 SLC30A4 2.02237 FHL2 -2.45574 MMP28 2.222272 LUNAR1 2.02113 KCNH2 -2.46409 TRAF5 2.221578 ATAT1 2.02076 NRGN -2.4646 MYO10 2.216708 RUNX1-IT1 2.01442 PTGS1 -2.46468 SETD7 2.204979 TRO 2.01351 GNAQ -2.46743 ARID5B 2.204345 RAB39B 2.01090 IL18 -2.4711 PEG10 2.203578 SIT1 2.00992 FOLR3 -2.48572 FLJ10038 2.203424 ABHD17B 2.00927 GYPE -2.49069 TERF2 2.202751 B3GNT8 -2.00038 ACKR1 -2.49475 LOC100130476 2.199619 KLHL2 -2.00175 ANO10 -2.49819 MYLK 2.197865 CDKN3 -2.00244 REPS2 -2.50515 MOV10 2.196604 SERPINB8 -2.00435 HLX -2.51686 EFNA1 2.19515 PXK -2.0072 HNRNPLL -2.52351 IFI16 2.19109 EVI5 -2.00954 MPP1 -2.52607 FHIT 2.190716 SQRDL -2.01001 PF4V1 -2.53928 SCAI 2.183901 CYB5R1 -2.01097 199

SUCNR1 -2.54364 ZBTB10 2.179607 UROD -2.0113 VIM -2.54899 LCN6 2.179336 DLC1 -2.01312 SLC25A37 -2.54985 BCR 2.177755 BRE-AS1 -2.01403 DEFB1 -2.55032 C1QTNF4 2.17744 TIMD4 -2.01665 PDGFC -2.55053 KIF16B 2.174262 SLC36A4 -2.01705 NME8 -2.55164 MRC1 2.167554 LHFPL2 -2.01756 TMCC2 -2.55548 INTS3 2.159915 HLA-DRB6 -2.02043 RAB31 -2.56497 TMEM156 2.154329 CX3CR1 -2.02248 TRIQK -2.57038 NEURL1B 2.142619 CCR7 -2.02292 NPL -2.57311 TPM4 2.137667 NPC2 -2.02318 CA3 -2.5744 LMF1 2.130996 TANGO2 -2.03338 TMEM71 -2.5772 CRAMP1 2.126179 CNIH4 -2.03352 BCL2L15 -2.57908 EFNB2 2.123747 SLC31A1 -2.03458 ANKRD9 -2.58713 HIST1H2BK 2.120382 LTA4H -2.03857 ABCB10 -2.59735 STAP1 2.118059 TPP1 -2.03866 CD177 -2.59749 ANKRD10-IT1 2.114551 TCL1A -2.04016 TRAK2 -2.60466 TTC7A 2.100596 ENPP4 -2.04063 DPY19L1P1 -2.60949 GJC1 2.098842 ATG7 -2.04645 AKR1C2 -2.61151 ZNF706 2.097937 ICAM4 -2.04767 H1F0 -2.614 NRXN3 2.095758 DEF8 -2.04922 SVIP -2.61619 HRK 2.094604 C20orf27 -2.05272 TESC -2.62782 HECTD2 2.093659 LEPROT -2.05433 RRAGD -2.64088 PPP2R2C 2.09137 NINJ2 -2.05536 CLEC4D -2.64115 MARCKSL1 2.088637 KCTD12 -2.05688 IL6R -2.64236 MDM2 2.087443 C1orf106 -2.05781 CPNE3 -2.64474 FBRSL1 2.086794 SIRPB2 -2.05787 TSPAN2 -2.65035 FAIM 2.086124 LAMP1 -2.05839 CPOX -2.65076 MEF2A 2.085028 IL1B -2.05915 MCEMP1 -2.65611 CEL 2.084132 SPI1 -2.05943 MNDA -2.65767 DGKE 2.082954 LAPTM5 -2.05996 OLFM4 -2.6591 H2BFS 2.08075 MAF -2.06378 CST7 -2.6654 SPTAN1 2.075357 CCND2 -2.06439 MGST1 -2.68139 FOXO1 2.071597 SLC14A1 -2.06478 KBTBD11 -2.69334 CHML 2.070903 S100A4 -2.0667 RNF24 -2.69529 CLN8 2.070776 DPPA4 -2.0699 FAM13A -2.69642 LOC202181 2.07018 IGF2BP2 -2.07049 IGFBP7 -2.69879 PARP15 2.069095 H1F0 -2.071 PDZK1IP1 -2.70291 FAM171A1 2.066129 ADAP1 -2.07118 RETN -2.703 ETS2 2.065804 SERPINB1 -2.07174 MICAL2 -2.70824 CMTM7 2.063837 G6PD -2.07219 RNF182 -2.71112 HIST1H2BC 2.06154 CARD6 -2.07325 RAB13 -2.72848 LOC728175 2.061102 PREX1 -2.07505 FBN2 -2.73021 EIF3F 2.057711 PLCG2 -2.07573 VCAM1 -2.73272 FERMT2 2.057265 FLCN -2.07583 FHDC1 -2.74502 RAB15 2.056903 MICAL2 -2.07937 200

RAB32 -2.74826 ZNF738 2.050348 CCDC126 -2.08018 YPEL4 -2.75528 P4HA2 2.046484 LTB4R -2.0804 ANXA4 -2.76168 SPPL2B 2.042696 PHACTR1 -2.08099 IGF2BP2 -2.76172 PKD2 2.04228 TPRG1L -2.08129 CPA3 -2.77113 ASMTL 2.034115 FGFR1OP2 -2.08411 SLC2A3 -2.77153 ZC4H2 2.02945 GRB10 -2.08757 STX3 -2.77172 DNMT3A 2.027744 HPSE -2.08895 APP -2.77189 LRP5 2.025789 ZDHHC3 -2.08959 EPOR -2.77251 CDKN2A 2.02413 B3GNT5 -2.09113 S100A12 -2.77387 B4GALT6 2.021074 MAP3K7CL -2.09279 MBOAT2 -2.77676 UBXN10-AS1 2.020298 GCLM -2.09646 KLF3 -2.77736 GNAS 2.016151 COL1A1 -2.09647 FAM210B -2.77827 GPR176 2.014671 BRI3 -2.09721 MINPP1 -2.77952 LARGE1 2.01408 ANKRD9 -2.09733 MLC1 -2.78147 CD81 2.011959 EFHD2 -2.09768 PLBD1 -2.78629 TRIO 2.011396 MBP -2.09868 CEBPE -2.79767 NRIP1 2.008592 SLCO3A1 -2.10062 MFAP3L -2.80124 CREB3L4 2.008131 LXN -2.10211 ITGAM -2.80266 MACF1 2.006063 GLMP -2.10245 EPX -2.80444 NARFL 2.005811 ATP6V0D1 -2.10293 STRADB -2.80867 RCBTB2 2.003937 C10orf128 -2.10362 SLC22A15 -2.81169 DGKH 2.001177 ALOX12 -2.10405 LPCAT2 -2.81437 SLC43A1 -2.00219 LGALS2 -2.10579 B3GNT5 -2.81496 G6PD -2.00497 IFNGR1 -2.1077 TM6SF1 -2.81531 NAALADL1 -2.00732 FAM201A -2.10924 AKR1C1 -2.81999 IDS -2.00955 CPEB2 -2.11047 MGAM -2.8246 MS4A4A -2.01223 CEBPB -2.11276 FCER1A -2.82812 TUBG1 -2.01313 NLRP3 -2.11411 FOSB -2.84081 NR4A2 -2.01762 UBAC1 -2.11489 DNTT -2.84491 BCL11B -2.01783 FAM214B -2.11693 PTGS2 -2.84727 UAP1 -2.01805 ZNF516 -2.11785 ACTN1 -2.8525 GSR -2.01903 VAMP3 -2.1185 RFLNB -2.8544 PELO -2.01911 CAPN2 -2.12169 HBQ1 -2.85485 HMMR -2.02248 TMEM30A -2.12285 GPR84 -2.85768 WLS -2.02322 SPECC1 -2.12342 LYZ -2.86189 GZMH -2.02545 TMEM64 -2.12551 CNRIP1 -2.86251 LHFPL2 -2.02593 DENND1A -2.12595 CTDSPL -2.86592 GRK6 -2.02678 KCNQ1 -2.12805 PROK2 -2.86766 CD3D -2.02699 CHPT1 -2.13025 SYNE1 -2.87278 ARAP3 -2.02822 COQ2 -2.13191 DMTN -2.87559 MCL1 -2.02844 CCNDBP1 -2.13255 EIF4E3 -2.87867 MAP3K7CL -2.02864 PDE4D -2.13278 SLC25A21 -2.89674 ALDH3A2 -2.03 LOC643072 -2.1329 SMIM24 -2.90021 CHAC2 -2.03332 MOSPD1 -2.13375 MS4A6A -2.90249 ATP2C2 -2.03504 ITPRIP -2.13408 201

RNASE2 -2.90265 SPARC -2.03768 B3GALNT1 -2.13665 PAQR9 -2.91139 ANLN -2.03786 CD101 -2.13693 TMOD1 -2.91829 PTH2R -2.03927 ARL4A -2.13705 NFIA -2.92064 TRAK2 -2.04121 PNPLA4 -2.13774 TMEM55A -2.94226 PATL1 -2.04125 AURKA -2.14264 CLEC12A -2.9426 VEPH1 -2.04187 PBX1 -2.14296 SYNGR1 -2.95136 TRMT6 -2.04414 MS4A1 -2.14457 BLVRB -2.95415 YME1L1 -2.04449 IRF8 -2.1473 FGF13 -2.95865 ABCC13 -2.04676 CCDC125 -2.14839 GMPR -2.96398 VPS8 -2.05037 MAN1A1 -2.14886 COL17A1 -2.97548 IRAK3 -2.05286 ATP6V1C1 -2.15564 NINJ2 -2.98726 ATG7 -2.0531 CNN2 -2.15568 STOM -2.99943 CD247 -2.055 CREBL2 -2.16182 RBP7 -3.00104 DIAPH2 -2.05511 SLC16A14 -2.16407 TSPAN5 -3.00165 SLC45A4 -2.05589 SDC4 -2.16456 FAM46A -3.01082 RNASE6 -2.0569 DLGAP5 -2.16485 AMPD3 -3.02276 GALNT3 -2.0582 ARSD -2.16562 PYGL -3.02419 SPTA1 -2.05945 SLC26A8 -2.16751 GJB6 -3.0352 C1orf106 -2.05959 CPM -2.16873 FAM46C -3.0495 ZFPM1 -2.06347 NQO2 -2.16935 KLF5 -3.05149 ABHD2 -2.06355 ZEB2 -2.17033 TFF3 -3.05209 PDE4D -2.06528 PLCL2 -2.17174 GSN -3.06564 SIGLEC10 -2.06763 QSOX1 -2.17272 CRISP2 -3.07884 CCNB1 -2.07195 PLAC8 -2.17503 SLC28A3 -3.07887 LYN -2.07234 FUT4 -2.17566 MYL4 -3.0876 LOC105377276 -2.07954 CREG1 -2.1796 SPG20 -3.09717 TNFAIP8 -2.08155 ADAM28 -2.1802 LGALSL -3.09754 KIAA0485 -2.08209 DUSP6 -2.18159 1-Mar -3.10355 CPEB2 -2.0825 TTC8 -2.18164 DNAJA4 -3.10447 KCNQ1 -2.08273 TAGAP -2.18249 PIWIL4 -3.12346 CAPN2 -2.0865 CDK14 -2.1838 STEAP3 -3.13415 SECTM1 -2.08775 MB21D1 -2.1839 GPR27 -3.13939 TET2 -2.08849 PTGER2 -2.18583 RFESD -3.15197 NXF3 -2.08867 HYAL3 -2.18764 CLTCL1 -3.15381 IL18R1 -2.08906 HAGH -2.18851 ANK1 -3.1683 MXI1 -2.08965 TFEB -2.18886 QPCT -3.17063 S100A4 -2.08974 MYOF -2.19 CLEC5A -3.17487 CCNE1 -2.09103 ZBP1 -2.19047 SLPI -3.18027 DMXL2 -2.09195 MEF2C -2.19266 RGL4 -3.19195 CD300A -2.09667 TGFBR1 -2.19283 BST1 -3.19608 LMNA -2.09704 GYS1 -2.19302 CEACAM1 -3.19767 BAG4 -2.0987 KLRF1 -2.1962 EPCAM -3.19864 PLEKHO2 -2.10001 NUDT19 -2.19735 CDH1 -3.21996 CNN2 -2.10001 PPP3CA -2.20068 SEC14L4 -3.23525 PTGS1 -2.10221 TMBIM6 -2.2017 202

LIN7A -3.24346 RAB20 -2.10285 SCO2 -2.20245 GPAT3 -3.25156 TSPAN5 -2.10538 PLPP3 -2.20715 VSTM1 -3.25786 PLXDC2 -2.1056 IL17RA -2.20728 SLC14A1 -3.26092 KLRF1 -2.10678 ITGB3 -2.2075 BEX1 -3.27253 RFLNB -2.10685 WARS -2.20989 IGKC -3.27284 CRLS1 -2.10876 OSM -2.21302 ACSL6 -3.31482 ADGRE1 -2.10912 ARAP3 -2.21332 FAXDC2 -3.32507 BIRC3 -2.1102 PDZD8 -2.21454 C17orf99 -3.33543 STOX2 -2.11201 GPCPD1 -2.21719 HP -3.3436 VAMP3 -2.11532 GZMA -2.21941 PRG2 -3.34578 TNFRSF1B -2.11883 RNASE6 -2.21964 CDA -3.39072 SELP -2.12087 ZNF185 -2.22027 CHIT1 -3.40645 STAT3 -2.1217 TMEM9B -2.22127 HBD -3.41324 ARHGAP24 -2.12518 LILRA6 -2.2213 GAS2L1 -3.41775 3/2/2017 -2.12639 FCGRT -2.22177 CD59 -3.42811 TMEM158 -2.12704 TMEM71 -2.22522 OSBPL1A -3.43211 LST1 -2.12795 MYADM -2.22764 MSRB3 -3.4412 CCNA2 -2.12823 TMEM120A -2.2281 S100P -3.45294 RTN3 -2.13008 CALD1 -2.23506 CYBRD1 -3.46832 PIP4K2A -2.13032 LAMP2 -2.23581 SOX6 -3.47054 MAPK14 -2.13352 PLBD2 -2.23638 KEL -3.495 NMNAT3 -2.13505 ATP6V0A1 -2.23686 CR1L -3.49894 UROD -2.13526 AOAH -2.23986 LOC10192804 FAR2 -3.50605 MAPKAPK3 -2.13551 7 -2.24012 CLEC11A -3.5216 TSPAN32 -2.13625 PLXDC2 -2.24186 FECH -3.55353 SPNS3 -2.13869 AK1 -2.24426 CA1 -3.57209 GPCPD1 -2.13872 3/2/2017 -2.2457 F13A1 -3.57319 HIST3H2A -2.14096 KBTBD11 -2.24572 CHI3L1 -3.58618 ZYX -2.14316 EGR1 -2.24679 SLC6A8 -3.59419 RAC2 -2.14341 UBE2D1 -2.24779 PTX3 -3.59979 FAH -2.14369 SLC39A11 -2.24931 AKR1C3 -3.6183 CFLAR -2.14407 MFSD1 -2.24946 SLC40A1 -3.61842 TUBB2A -2.14454 SLC22A16 -2.25082 TACSTD2 -3.66599 DPEP2 -2.147 FAM49A -2.25137 TRIM58 -3.68611 HOOK1 -2.14868 DEFB1 -2.2524 CLC -3.69379 PCMT1 -2.1503 CTSA -2.25388 MMP9 -3.69899 LSP1 -2.15095 ZDHHC2 -2.25588 PCOLCE2 -3.69958 ATP1B1 -2.15243 FGFBP2 -2.25592 CTSE -3.69994 CPNE3 -2.1536 CACNA2D3 -2.25625 RHD -3.74374 PELI1 -2.15543 ANXA1 -2.26164 SPARC -3.78554 CITED2 -2.15858 LINC01268 -2.26198 LCN2 -3.80272 PAK1 -2.15926 C5orf30 -2.26402 TSPO2 -3.82535 MITF -2.16118 MAP3K8 -2.26488 CD36 -3.82968 RIOK3 -2.16301 PDK4 -2.26663 203

CA2 -3.83314 SLC25A39 -2.16321 GATA1 -2.26679 CYP4F3 -3.83845 MTMR11 -2.16565 ARHGEF40 -2.26843 SERPINB10 -3.8403 VPS37C -2.16688 MAP3K5 -2.26852 ATP8B4 -3.84384 PIR -2.1689 CD55 -2.27004 NFE2 -3.86094 BAG2 -2.16983 PHACTR2 -2.27037 SLC22A4 -3.8616 CEACAM21 -2.17045 HLA-DMB -2.27423 XK -3.8636 TLE3 -2.17126 KIAA0368 -2.27677 LTF -3.87539 CNIH4 -2.17228 LACTB -2.27828 PADI4 -3.87723 CXADR -2.17562 KLRD1 -2.2787 FCAR -3.89041 CDK1 -2.17769 PLEKHO2 -2.27958 ARG1 -3.90961 PTGES -2.17793 NFKBID -2.27977 GFI1B -3.92114 FAM201A -2.18282 SLC27A3 -2.28148 TMEM56 -3.95193 HEBP1 -2.18646 MCL1 -2.28181 OLR1 -3.96971 ZBP1 -2.18782 SHTN1 -2.28337 GGTA1P -4.00795 ERN1 -2.18921 ALAS1 -2.28479 MPO -4.02274 FUT4 -2.19196 CYBA -2.28518 HBM -4.03286 B3GNT8 -2.20197 ABCB6 -2.28728 RGS18 -4.05104 UBAC1 -2.20209 PRCP -2.28764 CAVIN2 -4.07339 SEC11C -2.20251 METRNL -2.28904 SELENOP -4.0922 DZIP1L -2.20286 PIK3AP1 -2.28956 TAL1 -4.09747 FAM127A -2.20392 FCRL1 -2.29071 SLC4A1 -4.11205 OSBP2 -2.21259 SIGLEC7 -2.29107 ECRP -4.12711 KRT1 -2.21393 UGGT2 -2.29135 DEFA4 -4.13643 GATA1 -2.21401 TTC7B -2.2919 ADD2 -4.14333 IGHD -2.21486 LILRB1 -2.29476 ABCA13 -4.1754 CD63 -2.21669 DAPP1 -2.29558 ELANE -4.22535 GALNS -2.21697 NADK -2.29582 CXCL12 -4.24694 TMBIM6 -2.21873 LDLRAP1 -2.29814 SLC22A16 -4.24867 POMGNT2 -2.22067 RGCC -2.29957 CEACAM8 -4.25435 BCL6 -2.22339 LST1 -2.30272 SPTA1 -4.25528 MICAL2 -2.22482 IGLJ3 -2.30369 KLF1 -4.26842 FGFBP2 -2.22702 GZMH -2.30413 PGLYRP1 -4.29779 ATPIF1 -2.22752 ICAM1 -2.30625 ANXA3 -4.33697 TNFAIP2 -2.22791 LOXL3 -2.30809 TCN1 -4.34259 GYPA -2.22954 CDC42BPA -2.30943 RNASE3 -4.36941 SAMHD1 -2.23179 SGK1 -2.31025 AHSP -4.37284 PXYLP1 -2.23427 LITAF -2.31135 AQP1 -4.43648 C8orf88 -2.24017 CD63 -2.31144 CEACAM6 -4.45166 CPEB4 -2.24086 GABARAPL1 -2.31272 CTSG -4.48032 ACP6 -2.24212 BLVRA -2.31353 EPB42 -4.59837 ITGB3 -2.24425 RASSF3 -2.31488 HEMGN -4.66 PMP22 -2.24458 PDLIM1 -2.31559 ALAS2 -4.68451 MAF -2.24748 SLC2A1 -2.31675 GYPB -4.70067 FAM129A -2.24942 ASAP1 -2.31753 TRIM10 -4.71419 GPR65 -2.24968 MXI1 -2.31784 204

RHAG -4.72799 C15orf48 -2.2515 GGH -2.31891 AZU1 -4.73256 SCRN1 -2.25535 PGD -2.31916 MS4A3 -4.77871 LOC101928047 -2.25641 PTPN18 -2.3199 ORM1 -4.79562 RRAGD -2.25687 BSG -2.32285 PRTN3 -4.84503 LNPK -2.25873 DSE -2.32418 PRG3 -4.86367 IVNS1ABP -2.25893 CD58 -2.32614 PPBP -4.88404 FGFR1OP2 -2.25931 PTPN12 -2.32874 SELENBP1 -4.91688 NELL2 -2.26359 CITED2 -2.32905 PF4 -4.93268 FAM13A -2.26361 AMMECR1 -2.32982 CRISP3 -4.95706 NIPAL2 -2.26675 FLOT2 -2.33522 GYPA -4.97065 DHRS13 -2.2698 RHOB -2.33564 MMP8 -5.01069 LYST -2.27031 PAK1 -2.33595 TNS1 -5.06519 THBS4 -2.27276 DCAF12 -2.33726 SNCA -5.1098 NAGA -2.27375 PIGK -2.33731 TUBB1 -5.47438 PPM1M -2.27467 LMO2 -2.34144 CAMP -5.48735 GCLC -2.27865 LRRC25 -2.34184 RUNX3 -2.281 RASGEF1A -2.34213 TYMP -2.28222 CPPED1 -2.3433 SLC6A6 -2.28322 SLC40A1 -2.3449 ICAM1 -2.28981 ABCC13 -2.34641 DCLRE1A -2.29161 DACH1 -2.3495 MGST3 -2.29357 CTSD -2.35353 PRCP -2.29365 XCL1 -2.35684 NNMT -2.29399 LRP10 -2.35748 IL17RA -2.29626 FAM105A -2.36059 LAPTM4B -2.2969 LTBP1 -2.36459 ICAM4 -2.29836 HLA-DRB4 -2.36849 HYAL3 -2.29977 MEGF9 -2.37527 FAM83D -2.30148 DTD1 -2.37866 CD101 -2.30354 ZYX -2.38082 SLC29A1 -2.30654 PLEK2 -2.38327 -2.30983 THBD -2.38362 SPTB -2.30993 GALC -2.38635 EHD1 -2.31317 SLC24A4 -2.38641 SDCBP -2.31595 ABCB10 -2.39131 CTSA -2.32007 LRP3 -2.39329 ITGAV -2.3222 FRAT2 -2.39374 NTNG2 -2.32225 CLCN4 -2.40276 LITAF -2.32378 TGFBR3 -2.40387 GALC -2.32537 CTBP2 -2.40399 RBM47 -2.32537 FOS -2.40413 RTN1 -2.32846 ARHGEF37 -2.40502 CTSZ -2.33352 CRTAP -2.4058 SESN3 -2.3346 FCGR2C -2.40645 PFKFB4 -2.33508 NNMT -2.4077 205

SLC26A8 -2.33918 MICALL2 -2.41076 KIF21B -2.34596 TMEM216 -2.41292 KLRD1 -2.34763 SLFN13 -2.41656 PLAGL1 -2.35018 SPTB -2.41705 OSM -2.3523 C10orf54 -2.4187 LTA4H -2.35337 WLS -2.41881 SSBP3 -2.36157 TBC1D8 -2.42208 DLGAP5 -2.36995 AMPD3 -2.42261 PLBD2 -2.37306 FOXO1 -2.4248 KBTBD7 -2.38197 CD74 -2.42739 TPP1 -2.38213 PLAUR -2.42791 DIRC2 -2.38462 CPD -2.43207 ALOX12 -2.38546 THBS4 -2.43317 BMS1P20 -2.38673 GALNS -2.4343 SLC39A11 -2.38713 CCR2 -2.43931 CACNA2D3 -2.38737 ICA1 -2.44013 PLEK2 -2.39324 ZNF467 -2.44205 ARHGEF37 -2.39409 IL2RB -2.44401 S100A8 -2.40829 COTL1 -2.44502 GPX3 -2.40899 HSPA6 -2.44525 SCO2 -2.41075 GPD1L -2.44748 C1QB -2.41185 C4orf32 -2.44841 TNS1 -2.41186 CSF2RA -2.44885 AURKA -2.41533 BORCS8 -2.45059 CCDC125 -2.41777 PSTPIP1 -2.45111 ACTN1 -2.41924 TMEM52B -2.45112 FCGR2C -2.42151 OSBP2 -2.45182 LOXL3 -2.4221 RAB20 -2.45203 PHLDA1 -2.42231 EIF4E3 -2.45246 TFRC -2.42406 HBEGF -2.45279 PAPSS2 -2.42418 EGLN1 -2.45372 LRRC75A -2.42495 SMIM5 -2.45512 ZDHHC3 -2.42651 IGHD -2.4571 OSBPL1A -2.42902 BTK -2.45751 ASGR2 -2.43053 WDFY3 -2.45773 IGLC1 -2.43863 SAMHD1 -2.45995 GPI -2.44586 CPVL -2.46165 MLLT3 -2.44672 PRSS21 -2.46551 ATP6V0A1 -2.44865 TFDP1 -2.46596 TMCC3 -2.45173 BPGM -2.46744 NOCT -2.4526 NELL2 -2.46823 PLAUR -2.4556 ITGA2B -2.46853 ABCB10 -2.45721 SLC25A39 -2.47165 THEM4 -2.45726 LILRA2 -2.47617 UBXN2B -2.45862 FAM127A -2.47708 206

SERPINE2 -2.46009 CHST15 -2.47971 BSG -2.46256 SPOPL -2.48026 HSPA6 -2.46553 OAT -2.48068 GLMP -2.46761 OGFRL1 -2.48339 CDKN3 -2.46989 RIOK3 -2.48757 ITK -2.46989 ERMAP -2.48979 TMEM154 -2.47145 ZG16B -2.49281 TANC2 -2.47319 ADAM9 -2.49662 OAT -2.47583 GPX3 -2.49727 CSGALNACT 3/1/2017 -2.47732 1 -2.50101 PLPP3 -2.47913 METTL7A -2.50255 LACTB -2.48176 GZMB -2.504 CORO1C -2.48482 IMPA2 -2.50416 STRADB -2.48905 ID2 -2.50757 NUDT19 -2.48965 KIAA1211L -2.51067 GABARAPL1 -2.49235 ZBTB38 -2.51779 TLR2 -2.49679 CYTL1 -2.52163 FABP4 -2.49699 PKP2 -2.52461 KIAA1211 -2.49838 DEGS1 -2.52499 S100A10 -2.5006 CLEC1B -2.52845 RNF217 -2.50304 SUSD3 -2.52883 PIP5K1B -2.50352 3/3/2017 -2.52886 TNFSF13 -2.50594 GCNT2 -2.53057 LAT -2.50621 PILRA -2.5321 UGGT2 -2.51042 C17orf58 -2.53558 ZBTB38 -2.51794 SNX9 -2.53996 PPIF -2.5212 TMCC3 -2.54057 MARCKS -2.52511 IGLV1-44 -2.54612 NLRP12 -2.52579 FBXO6 -2.54744 PGM1 -2.52817 TYMP -2.55139 MCTP1 -2.53252 ACTN1 -2.55141 FRMD3 -2.53482 BLNK -2.55288 DHRS7 -2.53779 SGMS1 -2.55408 SLAMF7 -2.53893 GZMK -2.5602 HNRNPLL -2.54024 VDR -2.56371 AGL -2.54163 APMAP -2.56915 ADAM28 -2.54165 APLP2 -2.56996 SLC2A3 -2.54611 CCL4 -2.57753 NLRP3 -2.54802 PTGR1 -2.58176 SGMS1 -2.54967 APOBR -2.59192 CD86 -2.5569 ASL -2.59377 MEGF9 -2.55706 RASSF2 -2.59456 PSAP -2.55961 TESC -2.5946 BPGM -2.56191 S100A6 -2.59635 207

CD2 -2.56255 TUBA4A -2.59641 CTSS -2.56261 PLXNC1 -2.59832 TIMP2 -2.56638 NLRP12 -2.60079 SELL -2.5682 GGA2 -2.60468 PRKAR2B -2.56916 ITGA9 -2.60477 SPG20 -2.5697 RPS6KA2 -2.60873 PECR -2.57395 ADM -2.60905 PNKD -2.57451 TKT -2.61023 TANK -2.57475 PIWIL4 -2.61076 C10orf54 -2.57615 FRY -2.61259 MGLL -2.57882 FOSL2 -2.61274 CD8A -2.57937 TLR4 -2.6172 ABCA5 -2.58024 SLAMF7 -2.61818 ALAS1 -2.58071 PKLR -2.61884 CARD16 -2.58253 FABP4 -2.62182 CORO2A -2.58263 KYNU -2.62196 TMCC2 -2.59001 RNF217 -2.62481 SLCO3A1 -2.59186 DNAJA4 -2.62625 ARL4C -2.59216 BMS1P20 -2.62983 TCEAL9 -2.59437 ANKLE1 -2.63127 CLIC2 -2.59721 JAML -2.63144 TMEM52B -2.59857 VPS37C -2.63514 PKP2 -2.60035 MGST3 -2.63904 SERPINB1 -2.60066 IRAK2 -2.6469 AMPD3 -2.60155 DPY19L1P1 -2.64779 FKBP1B -2.60237 BMX -2.65047 STAT4 -2.60621 SNX18 -2.65357 CXCL16 -2.60984 GALNT11 -2.65434 PKLR -2.61255 CA4 -2.65563 VDR -2.62014 GYPE -2.65801 CPD -2.62055 REEP5 -2.6593 ICAM3 -2.62265 CYTIP -2.66204 CCL4 -2.62852 UPP1 -2.66433 ERMAP -2.62968 PLSCR1 -2.66603 NATD1 -2.63358 AIG1 -2.66797 CSF2RB -2.63461 ANO10 -2.66918 GYPE -2.63492 FZD5 -2.67 DPPA4 -2.63536 FRMD3 -2.67081 MLC1 -2.63865 CLEC4A -2.67214 CLEC4A -2.6395 PLA2G4A -2.67952 CCR2 -2.63969 FGD2 -2.68033 ALDH1A1 -2.64075 STRADB -2.68135 PLA2G4A -2.64152 LMNA -2.68456 KCNK5 -2.64529 FXN -2.68637 GZMB -2.64833 IL13RA1 -2.68913 208

UGCG -2.65108 MSRB3 -2.69672 LPCAT2 -2.65124 LYN -2.69714 CYP4F2 -2.65286 DRAM1 -2.69861 PTPN7 -2.65402 TRAK2 -2.69938 CLEC1B -2.65564 SORT1 -2.69981 LGALS2 -2.65836 SLC36A1 -2.70185 LAMP2 -2.66024 CPOX -2.70244 DEFB1 -2.66243 GNG7 -2.7074 LGALS1 -2.66813 RIN2 -2.71009 C1QC -2.6733 GAPT -2.71045 CCPG1 -2.67646 ALAD -2.71119 CPPED1 -2.67953 FAS -2.71183 BLVRA -2.68065 LILRA5 -2.71218 AKTIP -2.68167 YPEL4 -2.71283 GNLY -2.68278 NATD1 -2.71663 ITGB2-AS1 -2.68743 NFIA -2.71707 ARL4A -2.68896 FGD4 -2.71913 SMIM1 -2.68956 STEAP4 -2.72005 IL10RA -2.6939 VCL -2.72552 THBD -2.69886 PTK2 -2.72809 PARVB -2.70411 SEL1L3 -2.72876 CEBPA -2.70479 GNLY -2.73908 TKT -2.70495 PFKFB4 -2.74028 MICALL2 -2.70813 LGALS12 -2.74338 CLEC4E -2.70909 IL10RA -2.74453 KCTD12 -2.70996 MS4A4A -2.74503 PRKCD -2.71191 AKTIP -2.74846 GNPDA1 -2.71279 RASSF4 -2.75154 ITGA2B -2.7224 LAPTM4B -2.75345 GYPB -2.7233 TET2 -2.75506 CR1 -2.72592 RIPK2 -2.7554 KIT -2.7271 CXCL8 -2.76058 CXCL3 -2.72906 ANXA4 -2.76998 CDC42BPA -2.72916 PXN -2.77336 FGD4 -2.73001 PNP -2.77515 FZD5 -2.7329 SUCNR1 -2.77791 TMEM176B -2.73774 CPNE3 -2.78725 AKR1C2 -2.74419 HMBS -2.79356 RAB33A -2.74563 FHDC1 -2.8001 CTSW -2.75189 CTSB -2.80011 TFDP1 -2.75361 SMIM1 -2.80197 DHCR7 -2.75693 PDE4B -2.80464 JDP2 -2.7641 CTSZ -2.80621 C12orf29 -2.77344 CIDEB -2.80733 CECR1 -2.77497 ITGB2 -2.81263 209

TMOD1 -2.77727 C1QB -2.81404 PIK3CB -2.78607 ERLIN1 -2.81499 SIGLEC7 -2.79216 HEBP1 -2.81626 RGCC -2.79369 EPCAM -2.81644 SMARCD3 -2.79524 SRXN1 -2.81964 DPY19L1P1 -2.79627 CTSH -2.82187 IL2RB -2.7972 C1QC -2.82222 ADAM8 -2.80876 CPNE2 -2.82509 KBTBD6 -2.81162 KIF1B -2.82644 SNTB1 -2.81596 MS4A7 -2.83227 PLS1 -2.82325 FAM107B -2.8361 PIWIL4 -2.82851 NAGA -2.84039 ROPN1L -2.82997 EHD4 -2.84371 LRRC4 -2.83029 SLC16A6 -2.84437 RAB3D -2.83812 SEMA4A -2.84798 LOC101928429 -2.83988 NACC2 -2.8486 SLC14A1 -2.84097 LPCAT2 -2.85208 TLR4 -2.84123 MAP7 -2.85247 SORT1 -2.84447 ALDH2 -2.85868 PGD -2.845 PTGS1 -2.86093 APLP2 -2.84506 RXRA -2.86136 ADRB2 -2.8456 CLIC2 -2.86155 ABCA13 -2.84666 IGHM -2.86185 NFIA -2.85458 HACD4 -2.86531 FRAT2 -2.85523 QKI -2.86684 ALAD -2.857 AAED1 -2.87114 PXN -2.85849 CAT -2.87225 VNN1 -2.85857 CXCL16 -2.87476 FAR2 -2.86428 SMPDL3A -2.87502 KLRB1 -2.86587 ROGDI -2.87962 SLC27A2 -2.86606 LATS2 -2.88121 PTPN22 -2.87291 RNF144B -2.88675 DNAJA4 -2.87386 GPR183 -2.88796 GCLM -2.87407 PFKFB3 -2.88839 S100A6 -2.87547 SNTB1 -2.89055 GNS -2.87747 LYST -2.89119 ACKR1 -2.8855 CD9 -2.8971 C17orf58 -2.89067 KIAA0513 -2.89934 HCK -2.90173 IGLC1 -2.90113 SLC36A4 -2.90243 RNF182 -2.90129 ZG16B -2.90599 FECH -2.90395 CCL5 -2.91195 CCDC50 -2.90667 INPP1 -2.9169 LRRC4 -2.90703 SPAG1 -2.91767 ADAM8 -2.91294 PDZD8 -2.91984 FES -2.91342 210

FHDC1 -2.92212 SLC43A1 -2.91995 GFI1B -2.92428 PPP1R3D -2.92331 WARS -2.92721 TFEC -2.92587 PRSS21 -2.92845 KLF4 -2.92636 MS4A7 -2.92921 ANKRD22 -2.93015 PAQR9 -2.92986 ACKR1 -2.93477 ALDH3B1 -2.93202 LY86 -2.93583 APMAP -2.93348 TST -2.93626 TPM1 -2.93382 CA3 -2.94438 IL18 -2.93425 DNAJC6 -2.94895 TES -2.93495 RGS2 -2.9559 TRIQK -2.9357 SLC27A2 -2.95753 ANK1 -2.94183 SPG20 -2.95777 ANO10 -2.94484 NCEH1 -2.95915 BCL2L15 -2.95178 ROPN1L -2.96067 RHOU -2.95401 SULF2 -2.96148 LINC01268 -2.95456 FHL2 -2.96436 ATXN1 -2.96223 SPARC -2.9673 GPR183 -2.9633 JAZF1 -2.97059 PF4V1 -2.96429 PF4V1 -2.972 YPEL4 -2.96452 IER3 -2.97761 IL6R -2.9675 KLF11 -2.98268 MAP7 -2.96878 TPM1 -2.98436 GCNT2 -2.97861 CHD7 -2.9856 SKAP2 -2.98333 CCPG1 -2.98989 RAB11FIP1 -2.98459 REPS2 -2.99409 FAM210B -2.98661 MMP25 -2.99808 SAMSN1 -2.98682 NFE2 -3.01158 RNF182 -2.99049 TUSC1 -3.013 FAM105A -2.99407 CPEB4 -3.02312 CSF2RA -3.0012 MYO1F -3.03687 JAG1 -3.00236 NR4A2 -3.03779 SLC15A3 -3.00518 CEACAM21 -3.03805 GALNT11 -3.00593 CTSS -3.0429 TSPAN2 -3.0148 SLC15A3 -3.0472 NKG7 -3.01523 MAFB -3.04767 SHTN1 -3.01795 ALDH3A2 -3.05064 MYO1F -3.02356 HLA-DPB1 -3.05217 BMX -3.02751 CTTN -3.05474 DACH1 -3.03432 CAST -3.05841 RXRA -3.03737 NAMPT -3.05949 SLC24A4 -3.03901 TMCC2 -3.06291 ERLIN1 -3.04516 PSTPIP2 -3.0676 SEMA4A -3.04553 SLC25A21 -3.06843 NDFIP1 -3.04607 SLCO4C1 -3.07202 211

MMP25 -3.04693 LRG1 -3.07405 VCAM1 -3.05189 C2orf88 -3.07406 DMTN -3.05573 KLF9 -3.08163 CPOX -3.05707 SLC2A3 -3.08259 ANXA5 -3.06488 BCL2L15 -3.08579 LRRC25 -3.06827 RAB13 -3.09191 JAZF1 -3.07487 SLC11A1 -3.0965 SQRDL -3.08083 PAQR9 -3.09881 CTTN -3.08125 FPR2 -3.10584 STEAP4 -3.08316 NFAM1 -3.10663 MAL -3.09134 SLC31A2 -3.10916 SRXN1 -3.09361 ASAH1 -3.11103 CA3 -3.10126 MLC1 -3.1134 PTPRJ -3.10362 GPR65 -3.11406 SLC7A7 -3.10398 GAS2L1 -3.1179 ENTPD1 -3.10562 CTNNA1 -3.12024 SLC25A21 -3.10854 FAR2 -3.12427 MPZL3 -3.11085 NOD2 -3.12462 FAS -3.11092 GNS -3.1251 APOC1 -3.113 HLA-DPA1 -3.12653 SLC4A1 -3.11416 PDZK1IP1 -3.12705 PIP4K2C -3.11507 HLA-DQB1 -3.12786 CTSL -3.11538 CEACAM3 -3.13981 TRIB3 -3.1211 NMU -3.14413 TSPO -3.12679 SGMS2 -3.14462 NOD2 -3.13021 RFESD -3.14632 QSOX1 -3.13355 FAM198B -3.14685 FYB -3.13509 FUCA2 -3.15368 RAB27B -3.13626 RGL4 -3.15375 SLC31A2 -3.1392 GYG1 -3.15694 ACSL1 -3.1488 AQP9 -3.16847 FBN1 -3.14881 TNFRSF1B -3.17344 GYG1 -3.15349 ACSL6 -3.17569 AQP1 -3.15497 ANPEP -3.18477 SLC36A1 -3.159 CTSL -3.18774 HMBS -3.15967 KANK2 -3.19219 FBN2 -3.16098 PDXK -3.1933 EPOR -3.16151 AREG -3.19802 CEBPB -3.16585 KLF3 -3.2067 C2orf88 -3.16865 EPOR -3.21244 RUNX2 -3.17081 SLC25A37 -3.21519 TRIB1 -3.1747 IL18 -3.22387 CEACAM3 -3.18617 TFF3 -3.22422 REPS2 -3.18979 ANXA5 -3.22776 SLC19A2 -3.1901 CAPN3 -3.23027 212

ASAH1 -3.19703 S100A9 -3.23329 IMPA2 -3.20073 NLRC4 -3.23581 CDC14B -3.20352 FCER1A -3.23759 HCAR3 -3.20507 MFAP3L -3.2393 RGS2 -3.21245 ARHGAP24 -3.23955 IER3 -3.2138 HCAR3 -3.24668 CXCL12 -3.21396 TNFAIP6 -3.25106 PDZK1IP1 -3.21468 NEK6 -3.25155 TMEM71 -3.21688 KIF13A -3.25288 AKR1C3 -3.21928 HBQ1 -3.25402 DHRS9 -3.2302 CXCR2 -3.25742 NMU -3.23128 FAM210B -3.25882 TMEM216 -3.23204 TUBB6 -3.2631 NCEH1 -3.23284 CD86 -3.26333 IL1R2 -3.23445 NME8 -3.26461 UPP1 -3.23674 SLC7A7 -3.26624 APOBEC3B -3.23889 TMEM55A -3.26831 LILRA5 -3.24858 OSCAR -3.27256 PILRA -3.25181 CDC14B -3.27327 MPP7 -3.26303 JDP2 -3.27394 AKR1C1 -3.26462 APOBEC3B -3.27525 RORA -3.26909 IMPACT -3.27936 MFAP3L -3.27019 S100A11 -3.2808 COTL1 -3.27289 GFI1B -3.28163 THBS1 -3.27334 APOC1 -3.28313 ADD2 -3.27413 CSF3R -3.28541 JAML -3.28173 SIRPA -3.29174 HBQ1 -3.2821 GLT1D1 -3.29609 LTBP1 -3.28447 SERPINB6 -3.29984 XCL1 -3.28742 IL1R2 -3.3008 CLTCL1 -3.29312 NKG7 -3.3034 SLA -3.29561 PLD1 -3.30512 APOBR -3.29706 PARVB -3.30623 MBOAT2 -3.29989 SESN3 -3.31329 ICA1 -3.30959 C10orf11 -3.31679 SOX6 -3.31852 F5 -3.32413 PLEK -3.32064 NA -3.32626 EIF4E3 -3.33094 BCL2A1 -3.33106 CEBPE -3.33125 TSPO -3.33284 FCGR2A -3.33303 DMTN -3.33462 RFESD -3.34107 KCNH2 -3.33738 FPR2 -3.34593 CLEC7A -3.3449 GRN -3.35734 IL6R -3.34799 FGF13 -3.35877 FBP1 -3.35236 SNCA -3.35933 RAB27A -3.35669 213

SELPLG -3.36045 VCAM1 -3.36244 HBD -3.36483 FCGR3B -3.36758 DTD1 -3.37102 SOX6 -3.36956 TFF3 -3.37309 HLA-DMA -3.37262 CSF3R -3.37887 PRAM1 -3.37894 ACSL6 -3.38172 CD163 -3.38527 PSTPIP1 -3.39019 FAM46C -3.39224 LRG1 -3.39027 TNFSF13 -3.39765 SUCNR1 -3.40113 CST3 -3.40205 CD300LF -3.41839 TNFRSF10C -3.4034 FECH -3.42313 SLC4A1 -3.40449 BEX2 -3.42341 INHBA -3.41121 TMEM55A -3.42587 IGFBP7 -3.41258 FAXDC2 -3.42821 FNDC3B -3.41504 NINJ2 -3.43505 VNN2 -3.41519 ABHD5 -3.43675 P2RY2 -3.4178 PSTPIP2 -3.44052 ALDH1A1 -3.42519 GMPR -3.44148 MINPP1 -3.42812 KCNE3 -3.44696 CEBPE -3.42927 RAB27A -3.44974 CYBRD1 -3.43005 VNN2 -3.46567 RBM47 -3.43314 ANKRD22 -3.47048 FGL2 -3.43756 MS4A6A -3.47851 ADGRG3 -3.43932 KIAA0513 -3.48494 PLEK -3.44179 SIRPA -3.49235 SYNGR1 -3.44411 RNF144B -3.49541 CYP1B1 -3.44414 SGMS2 -3.50008 DHRS9 -3.44806 TTC8 -3.50057 GSAP -3.4484 FRY -3.50152 MBOAT2 -3.45109 GAS2L1 -3.50427 CCL5 -3.4594 FAM46C -3.50442 IGSF6 -3.47102 CTSD -3.5191 GNG11 -3.47293 SELENOP -3.52386 MS4A6A -3.47352 C10orf11 -3.52753 FCGR2A -3.47812 SEC14L4 -3.53765 RHAG -3.48699 ASRGL1 -3.5421 PRKCD -3.49032 STXBP5 -3.54943 CLTCL1 -3.49052 SLCO4C1 -3.55689 CD300LF -3.49084 DNAJC6 -3.56149 ADRB2 -3.49476 NFAM1 -3.56339 SNCA -3.4961 NACC2 -3.56698 CYSTM1 -3.49963 CYBRD1 -3.57145 OSBPL1A -3.50493 SLC6A8 -3.5759 CRISP2 -3.50576 NA -3.59824 CFP -3.50641 LGALS12 -3.61277 PDGFC -3.51069 214

ENPP4 -3.61599 CPA3 -3.51305 MYL4 -3.62371 HLA-DRA -3.51615 CXCR2 -3.62464 CFD -3.51662 NME8 -3.62925 TRIM58 -3.51948 FHL2 -3.65119 PTAFR -3.52277 AGTRAP -3.65508 HEMGN -3.52751 SLC25A37 -3.65672 IRS2 -3.52823 NPL -3.67192 PRKAR2B -3.52971 RHAG -3.67444 G0S2 -3.53003 CD59 -3.67646 SERPINA1 -3.53807 CD33 -3.67765 TMOD1 -3.54 SMPDL3A -3.67988 FGF13 -3.54078 TNFSF13B -3.6911 ENTPD1 -3.54213 SRGN -3.70355 ABHD5 -3.54449 NLRC4 -3.70537 SCPEP1 -3.55376 SLC28A3 -3.70547 PADI2 -3.5574 NRGN -3.71303 VNN3 -3.55833 P2RY2 -3.7165 UGCG -3.56168 MAP3K13 -3.71755 ALOX5AP -3.56272 TSPO2 -3.74318 FGR -3.56858 C1orf162 -3.74919 MSRA -3.56957 CTSE -3.74966 TLR2 -3.56989 KEL -3.75132 CEBPA -3.57416 PADI2 -3.75834 TFR2 -3.57509 AREG -3.76314 GRN -3.59119 PTAFR -3.77492 CLEC4E -3.59251 C17orf99 -3.77528 SLC28A3 -3.59324 MINPP1 -3.77927 C17orf99 -3.59489 CYP1B1 -3.78433 TM6SF1 -3.59645 STEAP3 -3.79055 SKAP2 -3.60096 IL1R1 -3.79151 LRRK2 -3.60719 CD1D -3.79478 MANSC1 -3.61571 GLT1D1 -3.79547 KCNJ15 -3.61635 WIPI1 -3.79823 FAXDC2 -3.61685 TNFAIP6 -3.79914 THBS1 -3.62845 STOM -3.80197 FBN2 -3.62861 CD163 -3.80773 HAL -3.63855 NAMPT -3.81247 SEC14L4 -3.64147 FAM65C -3.81347 SPIDR -3.64907 MXD1 -3.81986 ACPP -3.65471 BLVRB -3.82896 TLR8 -3.66083 TFEC -3.82912 CRISPLD2 -3.67196 TNFRSF10C -3.83151 GMPR -3.68274 DYSF -3.83659 TREM1 -3.68997 NCF2 -3.83698 KLF5 -3.69926 215

SCD -3.83854 HMOX1 -3.70201 GLIPR2 -3.84204 MYL4 -3.70579 KCNJ15 -3.84336 CXCL1 -3.7063 TMEM170B -3.84813 ITGAM -3.70917 SLC11A1 -3.85232 IL1R1 -3.71017 CCR1 -3.85644 C3AR1 -3.71028 CR1L -3.85818 CD33 -3.71105 CRISP2 -3.8614 CKAP4 -3.71258 CXCL2 -3.86244 JCHAIN -3.71679 RHD -3.86254 CEBPD -3.73311 CTSC -3.87146 TYROBP -3.73803 FNDC3B -3.8869 KEL -3.74384 FBP1 -3.89505 CDH1 -3.74491 NQO2 -3.90058 CTSE -3.74623 IGKC -3.90222 ARHGEF12 -3.74685 HBM -3.9064 SIGLEC5 -3.75646 NFE2 -3.91021 VCAN -3.76219 OSCAR -3.91049 THEMIS2 -3.76771 FCGR3B -3.91768 DYSF -3.76919 ALOX5AP -3.92509 CES1 -3.77088 BIN2 -3.92714 RGS18 -3.77138 ADGRG3 -3.92899 JAG1 -3.77937 MANSC1 -3.92908 AGTRAP -3.79106 CDH1 -3.95207 FAM46A -3.79765 VNN3 -3.95783 APP -3.80419 CA2 -3.98418 PTGS2 -3.80849 SLC16A6 -3.99139 SRGN -3.81096 ITGB2 -4.01891 CNRIP1 -3.81401 CA4 -4.03878 SPTA1 -3.81491 S100A9 -4.04848 HBD -3.82502 CTSH -4.05059 IRAK3 -3.83007 F5 -4.07215 STEAP3 -3.8446 CFP -4.07631 PTPRJ -3.85866 MSRB3 -4.08024 TLR1 -3.86362 CYBB -4.08513 ABCA13 -3.87755 LOC10192842 CKAP4 -4.08766 9 -3.90148 BEX3 -4.09649 COL17A1 -3.906 PDGFC -4.10342 HCK -3.9116 HAL -4.10906 ELL2 -3.91849 IGSF6 -4.11808 WIPI1 -3.91867 FAM198B -4.122 SERPINB2 -3.92089 AOAH -4.13337 CCR1 -3.93495 COL17A1 -4.14854 FCHO2 -3.93808 GPR160 -4.16215 GAS2L3 -3.94612 216

FUCA2 -4.1624 TSPO2 -3.94809 CA1 -4.16386 CR1L -3.94868 SNX10 -4.17193 F13A1 -3.95027 CST3 -4.1812 ACSL1 -3.95028 FCGR1B -4.1825 LILRA3 -3.95136 ALDH2 -4.19088 TRIB1 -3.97386 SPIDR -4.19211 MARCKS -3.98672 TESC -4.20912 STX11 -3.99575 CXCL8 -4.2154 MSRB1 -4.00113 AQP9 -4.2182 FCGR1B -4.00947 SIGLEC5 -4.23842 EPX -4.02417 CLU -4.23997 C1orf162 -4.03118 CYSTM1 -4.24098 FAM65C -4.03213 B4GALT5 -4.27675 SLC6A8 -4.03881 ANPEP -4.27836 LIN7A -4.05553 MAFB -4.2785 STX3 -4.06166 PRAM1 -4.28358 PRRG4 -4.06877 CES1 -4.28731 ANK1 -4.07447 STX11 -4.30136 SYNE1 -4.07858 HEMGN -4.30677 ALOX5 -4.08303 MSRB1 -4.30952 LILRB2 -4.0832 TM6SF1 -4.31778 CD59 -4.09378 CEBPD -4.31985 HNMT -4.09451 PRRG4 -4.32687 ALDH3B1 -4.10157 LRRK2 -4.34171 CLEC4D -4.11584 EPX -4.36597 TNFSF13B -4.12232 EPCAM -4.37313 CLU -4.13479 MCEMP1 -4.39562 CSF2RB -4.13765 CPVL -4.41467 CD177 -4.13863 KCNH2 -4.41954 CA1 -4.14921 XK -4.42068 3/1/2017 -4.16369 PTGS2 -4.43023 EREG -4.16733 HMOX1 -4.43995 PYGL -4.18272 CRISPLD2 -4.45474 SIRPB1 -4.2033 AIG1 -4.45855 IL1RN -4.20385 ACPP -4.46695 FCER1G -4.22373 GPR84 -4.46755 LGALS3 -4.22733 RGS18 -4.46902 NPL -4.24879 INHBA -4.47533 IL18RAP -4.26319 TLR8 -4.47616 RETN -4.2647 LGALSL -4.49808 TGFBI -4.27092 DOCK5 -4.50141 LGALSL -4.27684 GCA -4.50448 HBM -4.28207 ITGAM -4.52396 PROK2 -4.2836 CFD -4.54502 IGKC -4.28903 217

AHSP -4.57046 BLVRB -4.28953 ALAS2 -4.57141 NRGN -4.29774 KLF1 -4.57727 MPEG1 -4.30076 MPEG1 -4.58718 GPR160 -4.31413 PYGL -4.59517 GPR84 -4.34476 HNMT -4.60931 B4GALT5 -4.35092 FGL2 -4.61155 VNN1 -4.35368 EPB42 -4.61742 BEX1 -4.35746 BCL2A1 -4.62728 MGAM -4.3733 TRIM10 -4.64471 BST1 -4.37884 CEACAM1 -4.64841 GYPA -4.38639 GPAT3 -4.65225 GPAT3 -4.3948 CST7 -4.65323 PIP5K1B -4.40521 C3AR1 -4.65641 C5AR1 -4.40803 LGALS3 -4.66172 CEACAM1 -4.42566 LIN7A -4.66237 CST7 -4.42922 CNRIP1 -4.66737 CXCL12 -4.43135 BASP1 -4.68874 GYPB -4.43603 PADI4 -4.6963 FCAR -4.45904 CLEC4D -4.70351 NCF4 -4.47256 ATP8B4 -4.71951 GSN -4.47369 CD177 -4.72003 CD14 -4.47784 KLF5 -4.72298 GPR27 -4.48123 IL1RN -4.72336 FOLR3 -4.49421 TYROBP -4.73012 RBP7 -4.49486 RGL4 -4.73114 VSTM1 -4.50862 CXCL1 -4.73135 GJB6 -4.51113 SYNE1 -4.74151 SLC22A15 -4.51115 GJB6 -4.75494 MAP3K13 -4.523 BST1 -4.79694 KCNE3 -4.53713 3/1/2017 -4.7977 GCA -4.53771 SELENBP1 -4.80221 HLX -4.53958 CPA3 -4.81573 RHD -4.55136 FOLR3 -4.82147 S100A12 -4.56067 SDPR -4.82727 FPR1 -4.57634 ANXA1 -4.84022 CYBB -4.58422 TAL1 -4.84151 HK3 -4.58683 TMEM56 -4.86309 OLR1 -4.58806 VCAN -4.86681 PADI4 -4.59517 LILRA3 -4.87543 CHIT1 -4.60987 S100A11 -4.90271 P2RY13 -4.62614 SERPINA1 -4.92816 TRIM10 -4.64333 SLC22A4 -4.93388 KLF1 -4.64567 FCAR -4.94134 SLC2A5 -4.65652 G0S2 -4.94623 CSTA -4.66156 218

SERPINB2 -4.9564 MCEMP1 -4.66241 OLR1 -4.95987 PCOLCE2 -4.66446 FCER1A -4.97041 XK -4.70741 PROK2 -4.97358 ATP8B4 -4.71658 SIRPB1 -4.97592 STOM -4.74049 IL18RAP -4.98454 FCN1 -4.77093 CLEC7A -4.99427 TMEM56 -4.77153 TREM1 -5.01543 CD24 -4.78172 RETN -5.01627 TNS1 -4.79313 PCOLCE2 -5.0489 NCF2 -4.79785 SERPINB10 -5.062 CLEC5A -4.81343 ECRP -5.07963 LYZ -4.8172 CLEC5A -5.10701 CLEC12A -4.82141 MGAM -5.17133 AQP1 -4.83354 FGR -5.19966 RNASE2 -4.85354 CHIT1 -5.24765 SELENOP -4.85359 TGFBI -5.28684 SERPINB10 -4.85396 CD36 -5.30749 SDPR -4.89417 PRG2 -5.32718 QPCT -4.92219 SLC40A1 -5.34347 AHSP -4.92732 VSTM1 -5.36015 ECRP -4.93154 RBP7 -5.36475 PRG2 -4.94652 CLEC12A -5.37369 RAB31 -4.97593 QPCT -5.41464 CA2 -4.97737 PTX3 -5.41531 PTX3 -4.98385 FCER1G -5.4239 SLC22A4 -4.98524 P2RY13 -5.42733 EPB42 -4.98655 FAM46A -5.48614 CYP4F3 -5.08024 EREG -5.52571 CD36 -5.10746 GGTA1P -5.52903 ADD2 -5.11506 HK3 -5.55371 BASP1 -5.11658 S100A12 -5.6062 HP -5.12968 CEACAM6 -5.62712 SELENBP1 -5.15989 GPR27 -5.63232 ALAS2 -5.20753 CD14 -5.64723 GGTA1P -5.24257 RNASE2 -5.69608 LTF -5.28227 HP -5.71071 MGST1 -5.28541 FPR1 -5.71592 PRG3 -5.31959 SLC22A15 -5.73849 ORM1 -5.35878 TUBB1 -5.74173 ARG1 -5.37264 ARG1 -5.75945 MMP9 -5.37422 C5AR1 -5.78327 CLC -5.39588 RAB31 -5.80423 S100P -5.40304 LTF -5.81098 MNDA -5.46505 PRG3 -5.81491 CHI3L1 -5.49732 219

SLPI -5.84343 CDA -5.52067 RAB32 -5.84625 SLPI -5.52198 BEX1 -5.8884 TACSTD2 -5.56649 CSTA -5.94749 PLBD1 -5.57173 CYP4F3 -5.97667 ANXA3 -5.5969 CHI3L1 -6.01671 TUBB1 -5.82247 LYZ -6.03483 PGLYRP1 -5.82292 MNDA -6.05367 CTSG -5.85979 OLFM4 -6.05707 LCN2 -5.87699 TACSTD2 -6.05838 OLFM4 -6.06011 CLC -6.10395 DEFA4 -6.11278 MGST1 -6.14151 ELANE -6.1259 ORM1 -6.19977 MPO -6.21017 MPO -6.27193 CEACAM8 -6.21053 S100P -6.27558 TCN1 -6.26604 MMP9 -6.33935 RNASE3 -6.30732 ANXA3 -6.36219 PF4 -6.37897 DEFA4 -6.39735 MS4A3 -6.39767 CDA -6.41194 CEACAM6 -6.46951 LCN2 -6.42839 MMP8 -6.47723 PGLYRP1 -6.43829 CAMP -6.50623 PF4 -6.49276 CRISP3 -6.70731 FCN1 -6.49783 PRTN3 -6.78491 CTSG -6.60026 AZU1 -6.79495 ELANE -6.65648 PPBP -6.96808 CEACAM8 -6.71623 TCN1 -6.7181 MS4A3 -6.74039 CAMP -6.81358 PRTN3 -6.82741 RNASE3 -6.97369 PLBD1 -6.98153 MMP8 -6.99871 PPBP -7.00914 AZU1 -7.35854 CRISP3 -7.40604

220

Appendix M

Master List of DEGs in B-ALL with t(8;14)

221

DEGs in the B-ALL with t(8;14) subset

Entrez Log Fold- Adusted p- Gene Symbol ID change value

ACTN1 87 -2.852502602 0.000001 ADD2 119 -4.143329181 0.000001 AEBP1 165 3.28449574 0.000001 ALAD 210 -2.298539705 0.000001 ALAS2 212 -4.68451253 0.000001 ALDH1A1 216 -2.24185851 0.000001 ALDH3B1 221 -2.149336653 0.000001 ALOX5AP 241 -2.054096218 0.000001 AMPD3 272 -3.022764083 0.000001 ANK1 286 -3.16829795 0.000001 ANXA1 301 -2.44745634 0.000001 ANXA3 306 -4.33696783 0.000001 ANXA4 307 -2.761678751 0.000001 APOC1 341 -2.23862491 0.000001 APP 351 -2.771891066 0.000001 AQP1 358 -4.43648495 0.000001 ARG1 383 -3.909607876 0.000001 ATP2B4 493 -2.337062935 0.000001 AZU1 566 -4.732562452 0.000001 BCL7A 605 2.062016757 0.000001 CEACAM1 634 -3.197665828 0.000001 BLK 640 4.060949935 0.000001 BLVRB 645 -2.954152103 0.000001 BMP7 655 2.526300502 0.000001 BST1 683 -3.196075846 0.000001 KLF5 688 -3.051490962 0.000001 TSPO 706 -2.272425123 0.000001 CA1 759 -3.572087079 0.000001 CA2 760 -3.833142786 0.000001 CAMK2D 817 2.787220921 0.000001 CAMP 820 -5.487345411 0.000001 CAPG 822 -2.202518722 0.000001 CAPN3 825 -2.305267575 0.000001 CAT 847 -2.093531718 0.000001 CCND2 894 -2.315929989 0.000001 CD19 930 3.782773175 0.000001 MS4A1 931 2.192436425 0.000001 MS4A3 932 -4.778706679 0.000001 222

CD22 933 2.535159719 0.000001 CD33 945 -2.372769837 0.000001 CD36 948 -3.829675427 0.000001 ENTPD1 953 -2.344494611 0.000001 CD58 965 -2.144159777 0.000001 CD59 966 -3.428113166 0.000001 CD79A 973 3.142616053 0.000001 CD79B 974 3.425172118 0.000001 CDA 978 -3.390719756 0.000001 CDH1 999 -3.219961391 0.000001 CEBPA 1050 -2.081688002 0.000001 CEBPE 1053 -2.797671708 0.000001 CEACAM8 1088 -4.254345654 0.000001 CHI3L1 1116 -3.586180416 0.000001 CHIT1 1118 -3.406454903 0.000001 CLC 1178 -3.693785364 0.000001 CNR1 1268 3.4819884 0.000001 COL17A1 1308 -2.97548388 0.000001 CPA3 1359 -2.771129855 0.000001 CPOX 1371 -2.650764208 0.000001 CR1L 1379 -3.498935234 0.000001 CSF3R 1441 -2.181152475 0.000001 CSTA 1475 -2.3235049 0.000001 CTSE 1510 -3.699939148 0.000001 CTSG 1511 -4.480317942 0.000001 DACH1 1602 -2.398992052 0.000001 AKR1C1 1645 -2.819988382 0.000001 AKR1C2 1646 -2.611506567 0.000001 DEFA4 1669 -4.136430341 0.000001 DMD 1756 2.396082105 0.000001 DTX1 1840 3.541891636 0.000001 E2F5 1875 2.585540819 0.000001 EBF1 1879 2.44301577 0.000001 ELANE 1991 -4.225350768 0.000001 SERPINB1 1992 -2.378387843 0.000001 CTTN 2017 -2.434776303 0.000001 EPB42 2038 -4.598369683 0.000001 STOM 2040 -2.999434524 0.000001 EPOR 2057 -2.772508688 0.000001 F13A1 2162 -3.57319008 0.000001 FBN1 2200 -2.069857165 0.000001 FBN2 2201 -2.7302116 0.000001 FCAR 2204 -3.890406582 0.000001 FECH 2235 -3.55352773 0.000001 FES 2242 -2.21296684 0.000001 223

FGF13 2258 -2.958649599 0.000001 FHL2 2274 -2.455736263 0.000001 FOSB 2354 -2.840808136 0.000001 FUT4 2526 -2.225017533 0.000001 GATA1 2623 -2.175764228 0.000001 GGTA1P 2681 -4.007953449 0.000001 GCLM 2730 -2.006237724 0.000001 GMPR 2766 -2.96398307 0.000001 GNAQ 2776 -2.467434978 0.000001 GPM6A 2823 2.700947052 0.000001 GPR27 2850 -3.139388661 0.000001 GRB10 2887 -2.030747853 0.000001 GSN 2934 -3.065641985 0.000001 GYPA 2993 -4.970654445 0.000001 GYPB 2994 -4.700674396 0.000001 GYS1 2997 -2.035999362 0.000001 H1F0 3005 -2.613999436 0.000001 HAGH 3029 -2.007099616 0.000001 HBM 3042 -4.03285919 0.000001 HBQ1 3049 -2.854849498 0.000001 HK3 3101 -2.317667556 0.000001 HLX 3142 -2.516860758 0.000001 HMBS 3145 -2.235212637 0.000001 HP 3240 -3.343603852 0.000001 ID3 3399 3.430575228 0.000001 IGF1R 3480 -2.277502265 0.000001 IGFBP7 3490 -2.698786324 0.000001 IGKC 3514 -3.272843436 0.000001 IL6R 3570 -2.642361199 0.000001 IL18 3606 -2.471102098 0.000001 INPP1 3628 -2.235861461 0.000001 ITGA2B 3674 -2.216800883 0.000001 ITGAM 3684 -2.802662233 0.000001 ITPR3 3710 2.003870687 0.000001 JAK2 3717 -2.095159184 0.000001 KCNH2 3757 -2.464094969 0.000001 KEL 3792 -3.495001782 0.000001 LAIR1 3903 -2.229308988 0.000001 LAMP2 3920 -2.027868605 0.000001 LCN2 3934 -3.802719134 0.000001 LGALS3 3958 -2.325488271 0.000001 FADS3 3995 2.242744105 0.000001 LRMP 4033 2.089421123 0.000001 CYP4F3 4051 -3.838445549 0.000001 LTF 4057 -3.875389948 0.000001 224

LYZ 4069 -2.861889952 0.000001 TACSTD2 4070 -3.665990474 0.000001 EPCAM 4072 -3.198643173 0.000001 MGST1 4257 -2.681386669 0.000001 MGST3 4259 -2.094631302 0.000001 MMP8 4317 -5.0106917 0.000001 MMP9 4318 -3.698985602 0.000001 MNDA 4332 -2.657671085 0.000001 MPO 4353 -4.022739977 0.000001 MPP1 4354 -2.526072025 0.000001 MSRA 4482 -2.186442538 0.000001 MYC 4609 2.17298663 0.000001 MYL4 4635 -3.087595629 0.000001 NA 4663 5.009511939 0.000001 CEACAM6 4680 -4.451658592 0.000001 NCF2 4688 -2.417420434 0.000001 NFIA 4774 -2.920644049 0.000001 NFE2 4778 -3.86093593 0.000001 NINJ2 4815 -2.987263584 0.000001 NRGN 4900 -2.464601501 0.000001 NUCB2 4925 -2.203763436 0.000001 OLR1 4973 -3.969710031 0.000001 ORM1 5004 -4.795623011 0.000001 P2RX5 5026 2.874889154 0.000001 P2RY2 5029 -2.332297242 0.000001 PAWR 5074 2.365784264 0.000001 PCDH9 5101 3.450646232 0.000001 PF4 5196 -4.932675746 0.000001 SERPINB6 5269 -2.01991328 0.000001 SERPINB10 5273 -3.840302723 0.000001 PKLR 5313 -2.234327732 0.000001 PPBP 5473 -4.884041202 0.000001 PPP1R3D 5509 -2.14155317 0.000001 SRGN 5552 -2.408487204 0.000001 PRTN3 5657 -4.845026293 0.000001 PTAFR 5724 -2.316673365 0.000001 PTGS1 5742 -2.464676446 0.000001 PTH2R 5746 -2.104376014 0.000001 QSOX1 5768 -2.05476812 0.000001 PTX3 5806 -3.59978738 0.000001 PYGL 5836 -3.024185539 0.000001 RAB13 5872 -2.728476373 0.000001 RAB27A 5873 -2.033021797 0.000001 RGS16 6004 3.49551102 0.000001 RHAG 6005 -4.727992416 0.000001 225

RHD 6007 -3.743738338 0.000001 RNASE2 6036 -2.902652239 0.000001 RNASE3 6037 -4.369410292 0.000001 RXRA 6256 -2.018570177 0.000001 S100A12 6283 -2.773874945 0.000001 S100P 6286 -3.452944244 0.000001 MAPK12 6300 2.243764082 0.000001 CLEC11A 6320 -3.521602579 0.000001 CXCL12 6387 -4.246944887 0.000001 SELENOP 6414 -4.092197861 0.000001 SLC2A3 6515 -2.771531087 0.000001 SLC4A1 6521 -4.112046991 0.000001 SLC6A8 6535 -3.594187899 0.000001 SLC14A1 6563 -3.26092248 0.000001 SLC22A4 6583 -3.861602249 0.000001 SLPI 6590 -3.180268988 0.000001 SMARCA4 6597 2.156194086 0.000001 SNCA 6622 -5.109802183 0.000001 SPARC 6678 -3.785537911 0.000001 SPIB 6689 2.575843053 0.000001 SPTA1 6708 -4.255278445 0.000001 ST14 6768 2.91634799 0.000001 STAT3 6774 -2.025181542 0.000001 STX3 6809 -2.771721252 0.000001 SVIL 6840 -2.377135099 0.000001 TAL1 6886 -4.09747115 0.000001 TCF4 6925 2.295735394 0.000001 TCN1 6947 -4.342585612 0.000001 TERT 7015 2.01277307 0.000001 TFF3 7033 -3.052085476 0.000001 TFR2 7036 -2.440766718 0.000001 TNS1 7145 -5.065188624 0.000001 TPM1 7168 -2.402581564 0.000001 CRISP2 7180 -3.078838326 0.000001 UCHL1 7345 4.283509642 0.000001 UGCG 7357 -2.374099854 0.000001 UROD 7389 -2.227773781 0.000001 VCL 7414 -2.180567942 0.000001 VIM 7431 -2.548987273 0.000001 XK 7504 -3.863604772 0.000001 ZNF185 7739 -2.250853407 0.000001 FZD5 7855 -2.24005465 0.000001 CLTCL1 8218 -3.153807844 0.000001 DYSF 8291 -2.18495014 0.000001 GFI1B 8328 -3.921141743 0.000001 226

CAVIN2 8436 -4.073391586 0.000001 CDC42BPA 8476 -2.399517991 0.000001 PPFIBP2 8495 2.099703913 0.000001 CST7 8530 -2.66539589 0.000001 AKR1C3 8644 -3.61830005 0.000001 HRK 8739 3.45732203 0.000001 SIGLEC5 8778 -2.301888382 0.000001 LIN7A 8825 -3.243461425 0.000001 KAT2B 8850 -2.005999824 0.000001 CPNE3 8895 -2.644739405 0.000001 SGCE 8910 3.161312024 0.000001 MGAM 8972 -2.824596662 0.000001 SELENBP1 8991 -4.916877983 0.000001 PGLYRP1 8993 -4.297792414 0.000001 PSTPIP2 9050 -2.446989759 0.000001 MAP7 9053 -2.359336648 0.000001 PDLIM1 9124 2.014072757 0.000001 SYNGR1 9145 -2.951360411 0.000001 REPS2 9185 -2.505149954 0.000001 B4GALT5 9334 -2.143682759 0.000001 MINPP1 9562 -2.779519696 0.000001 APOBEC3B 9582 -2.402907095 0.000001 MICAL2 9645 -2.708236266 0.000001 RNF144A 9781 -2.017581405 0.000001 MFAP3L 9848 -2.801236245 0.000001 KBTBD11 9920 -2.693336996 0.000001 AMMECR1 9949 -2.122486084 0.000001 GJC1 10052 2.298815826 0.000001 ABCC5 10057 -2.115147718 0.000001 ABCB6 10058 -2.205864238 0.000001 TSPAN5 10098 -3.001649221 0.000001 TSPAN2 10100 -2.65035048 0.000001 TRIM10 10107 -4.714186944 0.000001 FAM13A 10144 -2.696416363 0.000001 CTDSPL 10217 -2.865916246 0.000001 CRISP3 10321 -4.95706087 0.000001 CITED2 10370 -2.303483674 0.000001 PRG3 10394 -4.863668141 0.000001 SH2B2 10603 2.316080035 0.000001 ERLIN1 10613 -2.017314091 0.000001 IVNS1ABP 10625 -2.331501787 0.000001 GAS2L1 10634 -3.4177491 0.000001 IGF2BP3 10643 3.842438194 0.000001 IGF2BP2 10644 -2.761715497 0.000001 KLF1 10661 -4.268415211 0.000001 227

FAXDC2 10826 -3.32507033 0.000001 RAB32 10981 -2.748256799 0.000001 RAB31 11031 -2.564974536 0.000001 SLC2A6 11182 2.24348649 0.000001 RNF24 11237 -2.695285917 0.000001 TFEC 22797 -2.179813759 0.000001 ELL2 22936 -2.175778824 0.000001 PTGR1 22949 -2.201203779 0.000001 PEG10 23089 4.758647712 0.000001 SPG20 23111 -3.097166381 0.000001 MLC1 23209 -2.781470303 0.000001 SEL1L3 23231 2.167388585 0.000001 MTCL1 23255 2.363314781 0.000001 SYNE1 23345 -2.872780811 0.000001 ABCB10 23456 -2.597352024 0.000001 PADI4 23569 -3.877230957 0.000001 CLEC5A 23601 -3.174865965 0.000001 CADM1 23705 2.363724552 0.000001 QPCT 25797 -3.170633031 0.000001 GCA 25801 -2.331774867 0.000001 TRIM58 25893 -3.686106858 0.000001 CNRIP1 25927 -2.862512632 0.000001 PTPN22 26191 -2.104712997 0.000001 PHGDH 26227 2.047085229 0.000001 STAP1 26228 2.058908302 0.000001 PCOLCE2 26577 -3.69957962 0.000001 GPR160 26996 -2.023892395 0.000001 SIT1 27240 2.811210345 0.000001 RAB30 27314 2.48269924 0.000001 LGALSL 29094 -3.097541503 0.000001 C11orf21 29125 -2.415089001 0.000001 SLC40A1 30061 -3.618421563 0.000001 HEBP1 50865 -2.147629287 0.000001 ABHD5 51099 -2.28490437 0.000001 KLF3 51274 -2.77735575 0.000001 SLC25A37 51312 -2.549845498 0.000001 NME8 51314 -2.551640079 0.000001 AHSP 51327 -4.372840117 0.000001 AIG1 51390 -2.331888847 0.000001 TM6SF1 53346 -2.815314682 0.000001 GPR84 53831 -2.857682504 0.000001 FAM105A 54491 -2.396226538 0.000001 NEURL1B 54492 -2.168566892 0.000001 MANSC1 54682 -2.449886541 0.000001 FAM46C 54855 -3.049497056 0.000001 228

LPCAT2 54947 -2.814368847 0.000001 TESC 54997 -2.627818106 0.000001 WIPI1 55062 -2.331830468 0.000001 ANO10 55129 -2.498187652 0.000001 STEAP3 55240 -3.13415051 0.000001 SLC22A15 55356 -2.811692694 0.000001 HEMGN 55363 -4.660003176 0.000001 STRADB 55437 -2.808665675 0.000001 DNAJA4 55466 -3.104470374 0.000001 TMEM55A 55529 -2.942259935 0.000001 SOX6 55553 -3.470536546 0.000001 FAM46A 55603 -3.010821949 0.000001 BCAS4 55653 2.583104966 0.000001 FAR2 55711 -3.506049658 0.000001 CSGALNACT 1 55790 -2.372372518 0.000001 BEX1 55859 -3.272532211 0.000001 PDGFC 56034 -2.550529334 0.000001 SUCNR1 56670 -2.543643719 0.000001 RETN 56729 -2.702995658 0.000001 LXN 56925 -2.093387387 0.000001 AGTRAP 57085 -2.318739301 0.000001 ZNF608 57507 2.276909404 0.000001 NCEH1 57552 -2.27833748 0.000001 RRAGD 58528 -2.640882645 0.000001 BACH2 60468 2.037775983 0.000001 PROK2 60675 -2.867663278 0.000001 PRDM15 63977 2.669273756 0.000001 SLC28A3 64078 -3.078866046 0.000001 LRRC4 64101 -2.269925366 0.000001 MS4A6A 64231 -2.902486337 0.000001 RGS18 64407 -4.051038415 0.000001 3/1/2017 64757 -3.10354801 0.000001 MARCKSL1 65108 2.123990687 0.000001 TRAK2 66008 -2.604659472 0.000001 FCRL2 79368 2.753409464 0.000001 MICALL2 79778 -2.365819173 0.000001 PLBD1 79887 -2.786287699 0.000001 ATP8B4 79895 -3.843840857 0.000001 CYBRD1 79901 -3.468324737 0.000001 RUBCNL 80183 3.557420086 0.000001 ELL3 80237 2.511823599 0.000001 CPEB4 80315 -2.089296568 0.000001 NPL 80896 -2.573108587 0.000001 TUBB1 81027 -5.474384786 0.000001 229

FAM117A 81558 -2.010965674 0.000001 AMN 81693 2.751735164 0.000001 TLR10 81793 2.040809465 0.000001 FAM167A 83648 2.036025497 0.000001 ROPN1L 83853 -2.401281711 0.000001 C10orf11 83938 -2.218520495 0.000001 PRAM1 84106 -2.451792662 0.000001 CYSTM1 84418 -2.047497433 0.000001 NTNG2 84628 -2.00045449 0.000001 GPAT3 84803 -3.251562546 0.000001 FCRLA 84824 3.795755885 0.000001 POMGNT2 84892 -2.015599193 0.000001 RNFT2 84900 2.453541295 0.000001 LGALS12 85329 -2.218025533 0.000001 SLC22A16 85413 -4.248665968 0.000001 SLC25A21 89874 -2.896737903 0.000001 CEACAM21 90273 -2.401976212 0.000001 SPECC1 92521 -2.430016444 0.000001 HNRNPLL 92906 -2.52351085 0.000001 ARHGAP18 93663 -2.066489209 0.000001 ATPIF1 93974 -2.32086049 0.000001 ERMAP 114625 -2.422398027 0.000001 OSBPL1A 114876 -3.432113019 0.000001 OSBPL10 114884 2.666714629 0.000001 3/3/2017 115123 -2.035116427 0.000001 FCHO2 115548 -2.009356797 0.000001 FAM210B 116151 -2.778269445 0.000001 RBP7 116362 -3.001039485 0.000001 ANKRD22 118932 -2.443485048 0.000001 C11orf74 119710 -2.378520609 0.000001 TMEM52B 120939 -2.139631459 0.000001 ANKRD9 122416 -2.587129847 0.000001 MBOAT2 129642 -2.776761172 0.000001 EMB 133418 -2.059635791 0.000001 CMBL 134147 -2.325485774 0.000001 SIRPA 140885 -2.212066724 0.000001 MPP7 143098 -2.393359199 0.000001 PIWIL4 143689 -3.123456262 0.000001 GLT1D1 144423 -2.194345681 0.000001 BEST3 144453 2.968506787 0.000001 TTC7B 145567 -2.412223511 0.000001 TMEM56 148534 -3.951934572 0.000001 ZNF385B 151126 3.820518326 0.000001 ZNF827 152485 2.302335709 0.000001 BTNL9 153579 3.07388848 0.000001 230

MBOAT1 154141 -2.442213814 0.000001 RNF217 154214 -2.078750367 0.000001 ABCA13 154664 -4.175399927 0.000001 CLEC12A 160364 -2.942599268 0.000001 CLECL1 160365 3.106726142 0.000001 IFNLR1 163702 3.063471942 0.000001 RASSF6 166824 3.555035361 0.000001 DNAH10 196385 -2.214868988 0.000001 MCEMP1 199675 -2.656107249 0.000001 FAM129C 199786 2.201280239 0.000001 SPNS3 201305 -2.229924896 0.000001 TSPO2 222642 -3.82534595 0.000001 MSRB3 253827 -3.441201105 0.000001 MCOLN2 255231 3.514333766 0.000001 SVIP 258010 -2.616191773 0.000001 RGL4 266747 -3.191950422 0.000001 VSTM1 284415 -3.257857814 0.000001 SMIM24 284422 -2.900206937 0.000001 SEC14L4 284904 -3.235248412 0.000001 LINC01268 285758 -2.116715973 0.000001 TRIQK 286144 -2.570380639 0.000001 EIF4E3 317649 -2.878674703 0.000001 RFESD 317671 -3.151971681 0.000001 CLEC4D 338339 -2.641146728 0.000001 C1orf228 339541 -2.117981647 0.000001 TMEM173 340061 -2.062485957 0.000001 KIAA1211L 343990 -2.266557049 0.000001 PAQR9 344838 -2.911389711 0.000001 SLCO4C1 353189 -2.399767095 0.000001 RFLNB 359845 -2.854401668 0.000001 BEND4 389206 2.506533069 0.000001 ARHGEF37 389337 -2.075707965 0.000001 EML6 400954 3.212894269 0.000001 BCL2L15 440603 -2.579076666 0.000001 SMIM5 643008 -2.453040561 0.000001 ECRP 643332 -4.127111408 0.000001 LOC10004971 6 1E+08 -2.16708726 0.000001 LOC10012944 7 1E+08 2.532963508 0.000001 DPY19L1P1 1E+08 -2.609485236 0.000001 C17orf99 1E+08 -3.335432901 0.000001 HBD 1E+08 -3.413243845 0.000001 FCER1A 2205 -2.828121382 1.07088E-06 CYTL1 54360 -2.215932217 1.12873E-06 231

GAS2L3 283431 -2.011198365 1.19315E-06 CACNA2D3 55799 -2.099902048 1.31918E-06 MSRB1 51734 -2.195599598 1.34983E-06 MCTP1 79772 -2.312936218 1.3586E-06 GPR18 2841 2.103649893 1.57721E-06 PRG2 5553 -3.345775819 1.74588E-06 ICAM4 3386 -2.175355034 1.78431E-06 FRMD4B 23150 -2.148959849 1.8038E-06 ACSL6 23305 -3.314817319 1.91261E-06 MYADM 91663 -2.122807257 2.05863E-06 DCAF12 25853 -2.082306862 2.1424E-06 NR4A2 4929 -2.042369935 2.18594E-06 FHDC1 85462 -2.745016805 2.23142E-06 NATD1 256302 -2.0497034 2.54722E-06 INHBA 3624 -2.260782306 2.56639E-06 TCL1A 8115 4.15142482 2.56639E-06 PDZD8 118987 -2.294493546 2.57673E-06 HLA-DOB 3112 2.120855488 2.72347E-06 DUSP6 1848 -2.198910299 3.04033E-06 ACRBP 84519 -2.101591226 3.27997E-06 SGMS2 166929 -2.145883398 3.3002E-06 FRY 10129 -2.007547946 4.06456E-06 RGS13 6003 2.092105446 4.10735E-06 KIF1B 23095 -2.159817727 4.27257E-06 CES1 1066 -2.375890591 4.37146E-06 TMEM71 137835 -2.577203093 4.43971E-06 FOS 2353 -2.074828881 4.52204E-06 DMTN 2039 -2.875586226 4.6635E-06 DNAJC6 9829 -2.17084714 4.86801E-06 FYB 2533 -2.055098574 4.99342E-06 CLIC2 1193 -2.287399536 5.71823E-06 PTPRCAP 5790 2.070705893 6.00009E-06 B3GNT5 84002 -2.814957082 6.63981E-06 RHOU 58480 -2.105740871 6.71882E-06 CLEC4A 50856 -2.126616879 7.02635E-06 ERG 2078 -2.265549095 7.40905E-06 IGHM 3507 2.672220659 9.28518E-06 PAX5 5079 2.818946237 9.3651E-06 TMOD1 7111 -2.918291228 9.50433E-06 HAL 3034 -2.083285546 9.91355E-06 TMEM170B 1E+08 -2.396352909 1.00785E-05 LOC10192842 1.02E+0 9 8 -2.267409374 1.02992E-05 NNMT 4837 -2.260191727 1.12251E-05 PF4V1 5197 -2.539282676 1.18734E-05 232

CRISPLD2 83716 -2.301572609 1.30688E-05 ADGRG3 222487 -2.400955653 1.37536E-05 ITGA9 3680 -2.020446783 1.44481E-05 ZMYM6 9204 -2.199783249 1.6258E-05 YPEL4 219539 -2.755284954 1.72471E-05 SMIM3 85027 -2.063510295 1.76938E-05 CD72 971 2.780272347 1.92583E-05 CLU 1191 -2.148420856 1.92773E-05 BMX 660 -2.140828576 2.16466E-05 GYPE 2996 -2.490690475 2.1719E-05 RNF182 221687 -2.711120988 2.2675E-05 IL1R1 3554 -2.006738036 2.40948E-05 RAB27B 5874 -2.379728795 2.49789E-05 NLRP12 91662 -2.014396821 2.66024E-05 AQP3 360 -2.065691284 2.73477E-05 ACKR1 2532 -2.49474519 2.77735E-05 CA3 761 -2.574400162 3.40212E-05 TNFSF13B 10673 -2.077772879 3.59156E-05 PDZK1IP1 10158 -2.702912292 3.83632E-05 IKZF2 22807 2.435514554 3.91566E-05 LYST 1130 -2.024542906 3.96136E-05 P2RY13 53829 -2.10533932 4.16421E-05 RPS6KA2 6196 -2.022869402 4.96103E-05 PMAIP1 5366 2.213932443 5.25406E-05 DNTT 1791 -2.844907739 7.21231E-05 FPR1 2357 -2.077587329 8.1783E-05 POU2AF1 5450 2.732874354 8.1882E-05 HLA-DPA1 3113 2.11647055 8.29233E-05 STX11 8676 -2.22028629 8.73259E-05 GJB6 10804 -3.035198914 8.85727E-05 F5 2153 -2.09536269 9.27591E-05 KCNE3 10008 -2.077840376 9.8379E-05 IGHD 3495 -2.010742835 0.000101391 EPX 8288 -2.804441288 0.000124426 DHRS9 10170 -2.336592728 0.000132344 BPGM 669 -2.242807098 0.000154587 CMTM2 146225 -2.31423005 0.000162546 IRAK3 11213 -2.11259111 0.000172315 EPDR1 54749 -2.248265437 0.000173442 VCAM1 7412 -2.732716665 0.000190118 PHACTR2 9749 -2.03991855 0.00019578 TMEM158 25907 -2.108710314 0.000200519 FOLR3 2352 -2.485716437 0.000213998 PTGS2 5743 -2.847266768 0.000222017 IL18RAP 8807 -2.170649149 0.000224177 233

TNFRSF10C 8794 -2.061362745 0.000280112 TRAF5 7188 2.14281342 0.000340144 CALD1 800 -2.038373248 0.000392587 CXCL1 2919 -2.108708333 0.000475142 BLNK 29760 2.374892919 0.000504741 IL1RN 3557 -2.133873056 0.000529902 RASSF4 83937 -2.135371035 0.000591656 TMCC2 9911 -2.555483851 0.000668692 OLFM4 10562 -2.659098509 0.001101491 VNN3 55350 -2.013230192 0.001121447 DEFB1 1672 -2.550318665 0.001449309 NLRC4 58484 -2.019800281 0.001558963 CA4 762 -2.071011632 0.001934466 TNFAIP6 7130 -2.076202851 0.002139693 VCAN 1462 -2.011359345 0.002352654 CD177 57126 -2.597488784 0.002736027 KRT1 3848 -2.393767236 0.004047092 OAS1 4938 2.171425386 0.004340396 VNN1 8876 -2.083105096 0.010101823 CCR2 729230 -2.019125925 0.02320083

234

Appendix N

Master List of DEGs in pooled B-ALL without t(8;14)

235

DEGs from the pooled B-ALL without t(8;14) subset

Entrez Log Fold- Adjusted p- Gene Symbol ID change value

ACPP 55 -4.466952887 0.000001 ACTN1 87 -2.41923931 0.000001 ADA 100 2.844164303 0.000001 ADAM8 101 -2.808761199 0.000001 ADCY9 115 2.277683022 0.000001 ADD2 119 -3.274131958 0.000001 ADRB2 154 -2.845598699 0.000001 AEBP1 165 3.826968973 0.000001 AGL 178 -2.541630275 0.000001 JAG1 182 -3.002358384 0.000001 ALAD 210 -2.857004943 0.000001 ALAS1 211 -2.580711541 0.000001 ALAS2 212 -4.571410902 0.000001 ALDH1A1 216 -2.640747146 0.000001 ALDH2 217 -4.190882524 0.000001 ALDH3B1 221 -2.932016651 0.000001 ALDH3A2 224 -2.030004415 0.000001 ALOX12 239 -2.385458039 0.000001 ALOX5AP 241 -3.92508902 0.000001 AMPD3 272 -2.601545788 0.000001 ANK1 286 -2.941825917 0.000001 ANPEP 290 -4.278355506 0.000001 ANXA1 301 -4.840221974 0.000001 ANXA3 306 -6.362193421 0.000001 ANXA5 308 -3.064878511 0.000001 AOAH 313 -4.133370369 0.000001 BIRC3 330 -2.110197067 0.000001 APLP2 334 -2.845057185 0.000001 APOC1 341 -3.113001993 0.000001 FAS 355 -3.110917909 0.000001 AQP1 358 -3.15496738 0.000001 AQP9 366 -4.218198325 0.000001 AREG 374 -3.763139392 0.000001 ARG1 383 -5.759449636 0.000001 ASAH1 427 -3.197031402 0.000001 ASGR2 433 -2.430533387 0.000001 ATP1B1 481 -2.152428718 0.000001 ALDH7A1 501 2.317734276 0.000001 ATP6V0A1 535 -2.448651139 0.000001 AZU1 566 -7.358537478 0.000001 236

BCL2A1 597 -4.627279805 0.000001 BCL6 604 -2.22338559 0.000001 BCL7A 605 3.490274094 0.000001 BCR 613 2.177755328 0.000001 CEACAM1 634 -4.648405122 0.000001 BLK 640 4.036841191 0.000001 BLVRA 644 -2.680650748 0.000001 BLVRB 645 -3.828959506 0.000001 BMP2 650 2.526301819 0.000001 BMX 660 -3.027513638 0.000001 BPGM 669 -2.5619074 0.000001 BSG 682 -2.462562278 0.000001 BST1 683 -4.796937576 0.000001 KLF5 688 -4.722976093 0.000001 TSPO 706 -3.126792537 0.000001 C1QB 713 -2.411849983 0.000001 C1QC 714 -2.673304117 0.000001 C3AR1 719 -4.656412668 0.000001 C5AR1 728 -5.783266234 0.000001 LDLRAD4 753 3.11805573 0.000001 C21orf2 755 2.58981634 0.000001 CA1 759 -4.163855481 0.000001 CA2 760 -3.98418451 0.000001 CA3 761 -3.101262819 0.000001 CA4 762 -4.038775179 0.000001 CACNB3 784 3.161094545 0.000001 CAMP 820 -6.813578454 0.000001 CAPN2 824 -2.086496193 0.000001 RUNX2 860 -3.170811703 0.000001 CBFA2T3 863 2.7810189 0.000001 RUNX3 864 -2.280997634 0.000001 CCNA2 890 -2.128230706 0.000001 CCNB1 891 -2.071954716 0.000001 CCNE1 898 -2.09102715 0.000001 CD1D 912 -3.794784635 0.000001 CD2 914 -2.562547098 0.000001 CD3D 915 -2.026994681 0.000001 CD247 919 -2.054999271 0.000001 CD8A 925 -2.579367149 0.000001 CD9 928 2.959481936 0.000001 CD14 929 -5.647231382 0.000001 CD19 930 5.705483758 0.000001 MS4A3 932 -6.740388738 0.000001 CD22 933 3.776326345 0.000001 CD86 942 -2.556900745 0.000001 237

CD33 945 -3.677650147 0.000001 CD34 947 3.482346056 0.000001 CD36 948 -5.307488933 0.000001 SCARB1 949 2.734332514 0.000001 ENTPD1 953 -3.105616431 0.000001 CD59 966 -3.67646341 0.000001 CD63 967 -2.216687761 0.000001 CD72 971 3.928397631 0.000001 CD79A 973 3.72921509 0.000001 CD79B 974 3.707794902 0.000001 CD81 975 2.011959246 0.000001 CDA 978 -6.411937921 0.000001 CDK1 983 -2.177691446 0.000001 CDH1 999 -3.952066881 0.000001 CDK6 1021 2.360568875 0.000001 CDKN2A 1029 2.024129514 0.000001 CDKN3 1033 -2.469885032 0.000001 CEBPA 1050 -2.704791829 0.000001 CEBPB 1051 -3.165852935 0.000001 CEBPD 1052 -4.319853249 0.000001 CEBPE 1053 -3.331252774 0.000001 CEL 1056 2.084132162 0.000001 CES1 1066 -4.287312381 0.000001 CTSC 1075 -3.871461912 0.000001 CEACAM3 1084 -3.186166766 0.000001 CEACAM8 1088 -6.716227591 0.000001 RCBTB2 1102 2.003937395 0.000001 CHI3L1 1116 -6.016708796 0.000001 CHIT1 1118 -5.247650793 0.000001 CHML 1122 2.070903303 0.000001 LYST 1130 -2.270308875 0.000001 CLC 1178 -6.103952523 0.000001 CLU 1191 -4.239973227 0.000001 CLIC2 1193 -2.597207373 0.000001 TPP1 1200 -2.382129984 0.000001 CCR1 1230 -3.856443188 0.000001 CNN2 1265 -2.100014324 0.000001 COL5A1 1289 4.760098315 0.000001 COL17A1 1308 -4.148538143 0.000001 SLC31A2 1318 -3.139195615 0.000001 CPA3 1359 -4.815734428 0.000001 CPD 1362 -2.620547339 0.000001 CPOX 1371 -3.05706781 0.000001 CR1 1378 -2.725916636 0.000001 CR1L 1379 -3.858177458 0.000001 238

MAPK14 1432 -2.133521538 0.000001 CSF2RA 1438 -3.001201217 0.000001 CSF2RB 1439 -2.634606494 0.000001 CSF3R 1441 -3.378870679 0.000001 CSNK1E 1454 2.431911791 0.000001 VCAN 1462 -4.866805951 0.000001 CSRP2 1466 6.735985315 0.000001 CST3 1471 -4.181198901 0.000001 CSTA 1475 -5.947489766 0.000001 CTGF 1490 5.232748854 0.000001 CTSD 1509 -3.519100847 0.000001 CTSE 1510 -3.749661412 0.000001 CTSG 1511 -6.600263713 0.000001 CTSH 1512 -4.050593047 0.000001 CTSL 1514 -3.115382477 0.000001 CTSS 1520 -2.562610477 0.000001 CTSW 1521 -2.751891461 0.000001 CTSZ 1522 -2.333521231 0.000001 CXADR 1525 -2.175615827 0.000001 CYBB 1536 -4.085132203 0.000001 CYP1B1 1545 -3.784334383 0.000001 DACH1 1602 -3.034315175 0.000001 DBN1 1627 3.610414037 0.000001 AKR1C1 1645 -3.264620762 0.000001 AKR1C2 1646 -2.744185832 0.000001 DEFA4 1669 -6.397347452 0.000001 DEFB1 1672 -2.662431094 0.000001 CFD 1675 -4.54501562 0.000001 DHCR7 1717 -2.756930606 0.000001 DIAPH2 1730 -2.055107382 0.000001 DNMT3A 1788 2.027744015 0.000001 DNTT 1791 5.610305642 0.000001 DPEP1 1800 4.062541655 0.000001 RCAN1 1827 2.914642601 0.000001 EBF1 1879 5.648730705 0.000001 GPR183 1880 -2.963303716 0.000001 TYMP 1890 -2.282223078 0.000001 ECM1 1893 5.376436926 0.000001 PHC1 1911 2.345327072 0.000001 EFNA1 1942 2.195149977 0.000001 EFNB2 1948 2.123746527 0.000001 MEGF9 1955 -2.557055452 0.000001 ELANE 1991 -6.656481161 0.000001 SERPINB1 1992 -2.600660916 0.000001 ADGRE1 2015 -2.109121835 0.000001 239

CTTN 2017 -3.081251453 0.000001 ENG 2022 2.237673758 0.000001 SLC29A1 2030 -2.306542929 0.000001 EPB42 2038 -4.617423561 0.000001 DMTN 2039 -3.055731895 0.000001 STOM 2040 -3.801973418 0.000001 CLN8 2055 2.070775897 0.000001 EPOR 2057 -3.16151446 0.000001 EREG 2069 -5.525713372 0.000001 ERG 2078 2.964631792 0.000001 ERN1 2081 -2.189208552 0.000001 ETS2 2114 2.065804297 0.000001 ETV5 2119 2.443252774 0.000001 F5 2153 -4.072153665 0.000001 FABP4 2167 -2.496988394 0.000001 ACSL1 2180 -3.148800257 0.000001 FAH 2184 -2.143694702 0.000001 FBN1 2200 -3.148812862 0.000001 FBN2 2201 -3.160982149 0.000001 FBP1 2203 -3.895046712 0.000001 FCAR 2204 -4.941344774 0.000001 FCER1A 2205 -4.970407431 0.000001 FCER1G 2207 -5.42390005 0.000001 FCGR1B 2210 -4.182504052 0.000001 FCGR2A 2212 -3.333030013 0.000001 FCGR3B 2215 -3.917675323 0.000001 FCN1 2219 -6.497827081 0.000001 FECH 2235 -3.423128591 0.000001 FGF13 2258 -3.35877393 0.000001 FGR 2268 -5.199663285 0.000001 FHIT 2272 2.190715946 0.000001 FHL1 2273 3.114036471 0.000001 FHL2 2274 -3.651185734 0.000001 FKBP1B 2281 -2.602374938 0.000001 FOXO1 2308 2.071596844 0.000001 FLNB 2317 2.32404974 0.000001 FLT3 2322 3.699142634 0.000001 FLT4 2324 2.335450587 0.000001 FOLR3 2352 -4.821474487 0.000001 FPGS 2356 2.259846527 0.000001 FPR1 2357 -5.715919098 0.000001 FPR2 2358 -3.345926079 0.000001 FUCA2 2519 -4.162399384 0.000001 FUT4 2526 -2.191955362 0.000001 ACKR1 2532 -2.885495437 0.000001 240

FYB 2533 -3.135089224 0.000001 G6PD 2539 -2.004974655 0.000001 GAB1 2549 2.97703908 0.000001 GALC 2581 -2.325366072 0.000001 GALNS 2588 -2.216970862 0.000001 GALNT2 2590 2.289199677 0.000001 GALNT3 2591 -2.058198042 0.000001 GATA1 2623 -2.214011868 0.000001 GCNT2 2651 -2.978612821 0.000001 GGTA1P 2681 -5.529029038 0.000001 GCLC 2729 -2.278654348 0.000001 GCLM 2730 -2.874067701 0.000001 GMPR 2766 -3.441482871 0.000001 GNAI1 2770 3.343503456 0.000001 GNAS 2778 2.016150874 0.000001 GNG7 2788 2.704787685 0.000001 GNS 2799 -2.877469209 0.000001 GPI 2821 -2.445863919 0.000001 GPM6B 2824 2.5644473 0.000001 GPR27 2850 -5.63232023 0.000001 GRK6 2870 -2.026783615 0.000001 GPX3 2878 -2.40898618 0.000001 GRN 2896 -3.357335705 0.000001 NR3C1 2908 2.260494099 0.000001 CXCL1 2919 -4.731353114 0.000001 CXCL2 2920 -3.862438327 0.000001 CXCL3 2921 -2.729058037 0.000001 GSR 2936 -2.019025308 0.000001 GUCY1A3 2982 3.292082767 0.000001 GYG1 2992 -3.153492407 0.000001 GYPA 2993 -2.229541686 0.000001 GYPB 2994 -2.723296356 0.000001 GYPE 2996 -2.634915077 0.000001 GZMH 2999 -2.025446852 0.000001 GZMB 3002 -2.648326983 0.000001 HIST1H2BD 3017 2.689274665 0.000001 HAL 3034 -4.109057635 0.000001 HBM 3042 -3.90639535 0.000001 HBQ1 3049 -3.282103176 0.000001 HCK 3055 -2.901727104 0.000001 HK3 3101 -5.553707288 0.000001 HLA-DOA 3111 3.668848909 0.000001 HMBS 3145 -3.159665309 0.000001 HMMR 3161 -2.022483687 0.000001 HMOX1 3162 -4.439952007 0.000001 241

HNMT 3176 -4.609308206 0.000001 HP 3240 -5.710711067 0.000001 HSPA6 3310 -2.465533976 0.000001 HSPB1 3315 2.508379856 0.000001 ICA1 3382 -3.309585135 0.000001 ICAM1 3383 -2.289813814 0.000001 ICAM3 3385 -2.622650969 0.000001 ICAM4 3386 -2.298360925 0.000001 ID3 3399 4.225247741 0.000001 IDS 3423 -2.009554913 0.000001 IFI16 3428 2.191089779 0.000001 IGHD 3495 -2.21486201 0.000001 IGHM 3507 2.760965198 0.000001 IGKC 3514 -3.90221614 0.000001 IGLC1 3537 -2.438625074 0.000001 IL1R1 3554 -3.791509811 0.000001 IL1RN 3557 -4.723356391 0.000001 IL2RB 3560 -2.797201323 0.000001 IL6R 3570 -2.967502176 0.000001 CXCL8 3576 -4.215401894 0.000001 CXCR2 3579 -3.624638929 0.000001 IL10RA 3587 -2.693900309 0.000001 IL18 3606 -2.934252206 0.000001 IMPA2 3613 -3.20072953 0.000001 INHBA 3624 -4.475327506 0.000001 INPP1 3628 -2.916900092 0.000001 INSR 3643 2.802655175 0.000001 ITGA6 3655 2.238857613 0.000001 ITGA2B 3674 -2.722401022 0.000001 ITGAM 3684 -4.523961641 0.000001 ITGAV 3685 -2.322197799 0.000001 ITGB2 3689 -4.018906865 0.000001 ITGB3 3690 -2.244247206 0.000001 ITK 3702 -2.469888776 0.000001 JUP 3728 4.503304391 0.000001 KCNH2 3757 -4.41954342 0.000001 KCNJ15 3772 -3.843364209 0.000001 KCNQ1 3784 -2.082734107 0.000001 KEL 3792 -3.751321656 0.000001 KIT 3815 -2.727101962 0.000001 KLRB1 3820 -2.865872495 0.000001 KLRD1 3824 -2.347626041 0.000001 KRT1 3848 -2.213925115 0.000001 AFF3 3899 3.541537318 0.000001 LAMC1 3915 2.540780751 0.000001 242

LAMP2 3920 -2.66024139 0.000001 LCN2 3934 -6.428390462 0.000001 LGALS1 3956 -2.668132768 0.000001 LGALS2 3957 -2.658361651 0.000001 LGALS3 3958 -4.661720976 0.000001 LIG4 3981 2.301311671 0.000001 FADS3 3995 3.600531146 0.000001 LMNA 4000 -2.097036205 0.000001 LRMP 4033 2.65189784 0.000001 LRP6 4040 2.76160447 0.000001 LRP5 4041 2.025789118 0.000001 LSP1 4046 -2.150948536 0.000001 LTA4H 4048 -2.353366495 0.000001 CYP4F3 4051 -5.976673753 0.000001 LTBP1 4052 -3.284470695 0.000001 LTF 4057 -5.810980504 0.000001 LYN 4067 -2.072338649 0.000001 LYZ 4069 -6.034827263 0.000001 TACSTD2 4070 -6.058375048 0.000001 EPCAM 4072 -4.373132772 0.000001 MARCKS 4082 -2.525109977 0.000001 MXD1 4084 -3.819856273 0.000001 SMAD1 4086 2.396324364 0.000001 MAF 4094 -2.247483467 0.000001 MAL 4118 -3.091337739 0.000001 MCL1 4170 -2.028435775 0.000001 MDK 4192 3.030128634 0.000001 MDM2 4193 2.087443024 0.000001 MEF2A 4205 2.085028092 0.000001 MEF2C 4208 3.731819687 0.000001 MGST1 4257 -6.141510068 0.000001 MGST3 4259 -2.293573324 0.000001 CIITA 4261 3.61745805 0.000001 MITF 4286 -2.161175371 0.000001 MLLT3 4300 -2.446717299 0.000001 AFDN 4301 2.651084619 0.000001 MME 4311 4.533874212 0.000001 MMP8 4317 -6.99870834 0.000001 MMP9 4318 -6.339353449 0.000001 MNDA 4332 -6.053666653 0.000001 MOV10 4343 2.196603544 0.000001 CD200 4345 3.8092143 0.000001 MPO 4353 -6.271933187 0.000001 MRC1 4360 2.167553695 0.000001 MYO1B 4430 2.522954048 0.000001 243

MYO1F 4542 -3.023558853 0.000001 MXI1 4601 -2.089652414 0.000001 MYL4 4635 -3.623707999 0.000001 MYLK 4638 2.19786487 0.000001 MYO10 4651 2.216708384 0.000001 NA 4663 -3.59824205 0.000001 NAGA 4668 -2.273749346 0.000001 CEACAM6 4680 -5.627124598 0.000001 NCF2 4688 -3.836977424 0.000001 NELL2 4753 -2.263585595 0.000001 NFIA 4774 -2.854577708 0.000001 NFE2 4778 -3.91021264 0.000001 NINJ2 4815 -3.435049596 0.000001 NKG7 4818 -3.015234365 0.000001 NQO2 4835 -3.900580349 0.000001 NNMT 4837 -2.293992936 0.000001 NPY 4852 5.231315555 0.000001 NPR1 4881 3.461975987 0.000001 NRGN 4900 -3.713031486 0.000001 NT5E 4907 3.613405815 0.000001 NR4A2 4929 -2.017619788 0.000001 OAT 4942 -2.475832831 0.000001 OLR1 4973 -4.959871968 0.000001 ORM1 5004 -6.199767177 0.000001 OSM 5008 -2.352302494 0.000001 P2RY2 5029 -3.716500548 0.000001 PRDX1 5052 2.362679383 0.000001 SERPINB2 5055 -4.956396008 0.000001 PAK1 5058 -2.159263127 0.000001 PAX5 5079 4.394562239 0.000001 PCDH9 5101 3.369263755 0.000001 PCMT1 5110 -2.150301982 0.000001 PDE4D 5144 -2.065275758 0.000001 PF4 5196 -6.49276333 0.000001 PF4V1 5197 -2.964291308 0.000001 CFP 5199 -4.0763141 0.000001 PFKFB4 5210 -2.33507831 0.000001 PGD 5226 -2.845000714 0.000001 PGM1 5236 -2.528166153 0.000001 SERPINA1 5265 -4.928159761 0.000001 SERPINE2 5270 -2.460091761 0.000001 SERPINB10 5273 -5.061997124 0.000001 PIK3C2B 5287 3.296379404 0.000001 PIK3CB 5291 -2.786073043 0.000001 PIP4K2A 5305 -2.130319329 0.000001 244

PKD2 5311 2.042279606 0.000001 PKLR 5313 -2.612548727 0.000001 PKP2 5318 -2.60035312 0.000001 PLA2G4A 5321 -2.641518752 0.000001 PLAGL1 5325 -2.350180373 0.000001 PLAUR 5329 -2.455603586 0.000001 PLEK 5341 -3.320636316 0.000001 PLS1 5357 -2.823250213 0.000001 PMAIP1 5366 2.46343272 0.000001 PMP22 5376 -2.244582218 0.000001 POU2AF1 5450 3.161421997 0.000001 POU4F1 5457 3.822556568 0.000001 PPBP 5473 -7.009141283 0.000001 CTSA 5476 -2.320070807 0.000001 PPP2R2C 5522 2.091369725 0.000001 PPP3CC 5533 2.395480186 0.000001 PRCP 5547 -2.29364954 0.000001 SRGN 5552 -3.703548494 0.000001 PRG2 5553 -5.327178915 0.000001 PRKAR2B 5577 -2.569155896 0.000001 PRKCD 5580 -2.711914207 0.000001 PRKCE 5581 3.113794149 0.000001 PRKX 5613 2.585461036 0.000001 PRTN3 5657 -6.827413662 0.000001 PSAP 5660 -2.559605741 0.000001 PTAFR 5724 -3.774924702 0.000001 PTGS1 5742 -2.102207243 0.000001 PTGS2 5743 -4.430225459 0.000001 PTH2R 5746 -2.039272196 0.000001 QSOX1 5768 -3.133545446 0.000001 PTPN7 5778 -2.654018558 0.000001 PTPN14 5784 2.856919043 0.000001 PTPRCAP 5790 2.271561278 0.000001 PTPRJ 5795 -3.103620358 0.000001 PTX3 5806 -5.41531403 0.000001 PXN 5829 -2.8584863 0.000001 PYGL 5836 -4.595168488 0.000001 RAB27A 5873 -3.44974122 0.000001 RAB27B 5874 -3.136257701 0.000001 RAC2 5880 -2.14341247 0.000001 RAG1 5896 4.84836465 0.000001 RAG2 5897 2.903295759 0.000001 RGS2 5997 -3.212450599 0.000001 RHAG 6005 -3.674439445 0.000001 RHD 6007 -3.862544194 0.000001 245

RNASE2 6036 -5.696084846 0.000001 RNASE3 6037 -6.973692836 0.000001 RNASE6 6039 -2.056895951 0.000001 RORA 6095 -3.269092958 0.000001 RTN1 6252 -2.328464175 0.000001 RXRA 6256 -3.037367989 0.000001 SORT1 6272 -2.844472069 0.000001 S100A4 6275 -2.089738669 0.000001 S100A6 6277 -2.875469978 0.000001 S100A8 6279 -2.408287437 0.000001 S100A9 6280 -4.048478164 0.000001 S100A10 6281 -2.500603857 0.000001 S100A11 6282 -4.902711352 0.000001 S100A12 6283 -5.606196723 0.000001 S100A13 6284 3.733368554 0.000001 S100P 6286 -6.275581854 0.000001 ATXN1 6310 -2.962226952 0.000001 SCD 6319 -3.838540645 0.000001 SCN3A 6328 2.769476579 0.000001 CCL4 6351 -2.628518165 0.000001 CCL5 6352 -2.911951332 0.000001 XCL1 6375 -3.287417409 0.000001 SDC2 6383 2.456152615 0.000001 SDCBP 6386 -2.315946212 0.000001 CXCL12 6387 -3.213962597 0.000001 SECTM1 6398 -2.087753442 0.000001 SELL 6402 -2.568195879 0.000001 SELP 6403 -2.120874288 0.000001 SELPLG 6404 -3.360448408 0.000001 SELENOP 6414 -3.523857093 0.000001 SLA 6503 -3.295605745 0.000001 SLC2A3 6515 -2.546112627 0.000001 SLC4A1 6521 -3.114161147 0.000001 SLC6A6 6533 -2.283216892 0.000001 SLC6A8 6535 -3.575899992 0.000001 SLC11A1 6556 -3.852319761 0.000001 SLC14A1 6563 -2.840971951 0.000001 SLC22A4 6583 -4.933884782 0.000001 SLPI 6590 -5.843427403 0.000001 SMARCA4 6597 2.382561437 0.000001 SMARCD3 6604 -2.79524408 0.000001 SNCA 6622 -3.359334652 0.000001 SNTB1 6641 -2.815959632 0.000001 SOX4 6659 3.560516158 0.000001 SPAG1 6674 -2.917668074 0.000001 246

UAP1 6675 -2.018051021 0.000001 SPARC 6678 -2.037675301 0.000001 SPTA1 6708 -2.059454711 0.000001 SPTAN1 6709 2.075357309 0.000001 SPTB 6710 -2.309929251 0.000001 SPTBN1 6711 2.547025738 0.000001 STAT3 6774 -2.121699449 0.000001 STAT4 6775 -2.606206413 0.000001 AURKA 6790 -2.415329895 0.000001 TAL1 6886 -4.841507623 0.000001 TCF4 6925 4.162105522 0.000001 TCF3 6929 2.465815467 0.000001 TCN1 6947 -6.71810267 0.000001 TMBIM6 7009 -2.218727826 0.000001 TERF2 7014 2.202750801 0.000001 TFDP1 7027 -2.753611992 0.000001 TFF3 7033 -3.373086699 0.000001 TFRC 7037 -2.424064051 0.000001 TGFBI 7045 -5.286837883 0.000001 THBD 7056 -2.698864174 0.000001 THBS1 7057 -3.273337698 0.000001 THBS4 7060 -2.272759365 0.000001 TIMP2 7077 -2.56637835 0.000001 TKT 7086 -2.704952061 0.000001 TLE3 7090 -2.171255694 0.000001 TLR2 7097 -2.496790403 0.000001 TLR4 7099 -2.841225741 0.000001 TSPAN7 7102 3.315523359 0.000001 TMOD1 7111 -2.777270739 0.000001 TNFAIP2 7127 -2.227910289 0.000001 TNFAIP6 7130 -3.799135456 0.000001 TNFRSF1B 7133 -2.118826089 0.000001 TNS1 7145 -2.411862699 0.000001 TPM1 7168 -2.93382266 0.000001 TPM2 7169 2.711002487 0.000001 TPM4 7171 2.137666933 0.000001 CRISP2 7180 -3.8614004 0.000001 TRAF5 7188 2.221578461 0.000001 TRIO 7204 2.011396355 0.000001 TUBB2A 7280 -2.144543071 0.000001 TUBG1 7283 -2.013126229 0.000001 TNFSF4 7292 3.794925605 0.000001 TYROBP 7305 -4.730117681 0.000001 UGCG 7357 -2.651082615 0.000001 UPP1 7378 -3.236735691 0.000001 247

UROD 7389 -2.135263191 0.000001 VCAM1 7412 -3.051886604 0.000001 VDR 7421 -2.620138525 0.000001 VPREB1 7441 5.523950751 0.000001 WARS 7453 -2.927209798 0.000001 CLIP2 7461 3.949653453 0.000001 CORO2A 7464 -2.582630359 0.000001 WFS1 7466 3.842919784 0.000001 WT1 7490 2.266864796 0.000001 XK 7504 -4.420682813 0.000001 ZNF70 7621 2.627755327 0.000001 ZNF91 7644 2.746663313 0.000001 ZYX 7791 -2.143161521 0.000001 PXDN 7837 6.335652812 0.000001 IL1R2 7850 -3.234454027 0.000001 FZD5 7855 -2.732902866 0.000001 MAPKAPK3 7867 -2.135508355 0.000001 ALDH5A1 7915 2.361666926 0.000001 LST1 7940 -2.127952033 0.000001 TCL1A 8115 5.057323493 0.000001 NRIP1 8204 2.008591783 0.000001 CLTCL1 8218 -3.293115355 0.000001 USP11 8237 2.493748191 0.000001 EPX 8288 -4.365971429 0.000001 DYSF 8291 -3.836592798 0.000001 GFI1B 8328 -2.924276618 0.000001 HIST1H2AM 8336 2.560875798 0.000001 HIST1H2BC 8347 2.061540252 0.000001 HYAL3 8372 -2.29977227 0.000001 PIP5K1B 8395 -2.503518824 0.000001 SDPR 8436 -4.827270705 0.000001 DYRK2 8445 2.468028335 0.000001 CDC42BPA 8476 -2.729159808 0.000001 GPR65 8477 -2.249680645 0.000001 SLC43A1 8501 -2.002188836 0.000001 DGKE 8526 2.08295364 0.000001 CYP4F2 8529 -2.652863751 0.000001 CST7 8530 -4.653226002 0.000001 PIR 8544 -2.168901733 0.000001 LGR5 8549 2.331825889 0.000001 CDC14B 8555 -3.203521387 0.000001 PLPP3 8613 -2.479126904 0.000001 ASMTL 8623 2.034115317 0.000001 DCHS1 8642 3.242335595 0.000001 AKR1C3 8644 -3.219275974 0.000001 248

KCNK5 8645 -2.645289768 0.000001 EIF3F 8665 2.057710786 0.000001 STX11 8676 -4.301355701 0.000001 HRK 8739 2.094603521 0.000001 TNFSF13 8741 -2.505936545 0.000001 SIGLEC5 8778 -4.238418473 0.000001 RIOK3 8780 -2.163008518 0.000001 TNFRSF10C 8794 -3.831508712 0.000001 IL18RAP 8807 -4.984544144 0.000001 IL18R1 8809 -2.089062229 0.000001 LIN7A 8825 -4.662371804 0.000001 SOCS2 8835 4.668747243 0.000001 CFLAR 8837 -2.144074318 0.000001 HCAR3 8843 -3.205071259 0.000001 ASAP2 8853 2.30885272 0.000001 IER3 8870 -3.213798479 0.000001 ARHGEF7 8874 2.297776517 0.000001 VNN2 8875 -3.465674535 0.000001 VNN1 8876 -2.858572613 0.000001 CPNE3 8895 -2.15359751 0.000001 FAM127A 8933 -2.203918949 0.000001 SKAP2 8935 -2.983329407 0.000001 WASF1 8936 2.826588692 0.000001 MGAM 8972 -5.171332708 0.000001 P4HA2 8974 2.046484119 0.000001 USP13 8975 2.239158338 0.000001 SELENBP1 8991 -4.802212876 0.000001 PGLYRP1 8993 -6.43829239 0.000001 LIMD1 8994 2.382282562 0.000001 PSTPIP2 9050 -3.440521741 0.000001 PSTPIP1 9051 -3.390189388 0.000001 MAP7 9053 -2.968775526 0.000001 SLC7A7 9056 -3.103982958 0.000001 PAPSS2 9060 -2.42418373 0.000001 FCGR2C 9103 -2.421510036 0.000001 SLC16A6 9120 -3.991391702 0.000001 PDLIM1 9124 2.76901944 0.000001 MAP3K13 9175 -3.717545635 0.000001 REPS2 9185 -3.189787806 0.000001 HACD1 9200 2.22358845 0.000001 LARGE1 9215 2.014080216 0.000001 CCPG1 9236 -2.676456905 0.000001 KLF4 9314 -2.309826035 0.000001 NREP 9315 2.440376126 0.000001 B4GALT6 9331 2.021073938 0.000001 249

CD163 9332 -3.807731008 0.000001 B4GALT5 9334 -4.276746722 0.000001 VAMP3 9341 -2.115317042 0.000001 RAB33A 9363 -2.745629538 0.000001 NRXN3 9369 2.095757539 0.000001 NRXN2 9379 2.613014739 0.000001 CD101 9398 -2.303544753 0.000001 ARHGAP29 9411 2.25778382 0.000001 LITAF 9516 -2.323777073 0.000001 BAG4 9530 -2.098696916 0.000001 BAG2 9532 -2.169831387 0.000001 PTGES 9536 -2.177933264 0.000001 RAB3D 9545 -2.838119112 0.000001 MINPP1 9562 -3.779267017 0.000001 GTF2IRD1 9569 2.28487098 0.000001 APOBEC3B 9582 -3.238893033 0.000001 AKAP12 9590 2.884305985 0.000001 ARHGEF10 9639 2.958792886 0.000001 MICAL2 9645 -2.224824513 0.000001 KIAA0513 9764 -3.48494376 0.000001 JADE3 9767 3.02319242 0.000001 DLGAP5 9787 -2.369946062 0.000001 SCRN1 9805 -2.255349475 0.000001 DNAJC6 9829 -3.561494079 0.000001 MFAP3L 9848 -3.270193239 0.000001 TMCC2 9911 -2.590007123 0.000001 ATP2C2 9914 -2.035042649 0.000001 P2RY14 9934 2.238678472 0.000001 MAFB 9935 -4.278496358 0.000001 DCLRE1A 9937 -2.291611644 0.000001 SLC23A1 9963 3.049279646 0.000001 SCO2 9997 -2.410753227 0.000001 NAALADL1 10004 -2.007322251 0.000001 GNPDA1 10007 -2.712791752 0.000001 KCNE3 10008 -3.446955231 0.000001 TANK 10010 -2.574750599 0.000001 GJC1 10052 2.098842488 0.000001 TSPAN32 10077 -2.136250761 0.000001 TSPAN5 10098 -2.10537802 0.000001 TSPAN2 10100 -3.014796994 0.000001 PPIF 10105 -2.52119949 0.000001 TRIM10 10107 -4.644707073 0.000001 ARL4C 10123 -2.592162773 0.000001 ARL4A 10124 -2.688957031 0.000001 FRY 10129 -3.501517527 0.000001 250

NAMPT 10135 -3.812465439 0.000001 FAM13A 10144 -2.263605667 0.000001 PDZK1IP1 10158 -3.214681808 0.000001 LPAR6 10161 3.090575232 0.000001 DHRS9 10170 -3.230203584 0.000001 LHFPL2 10184 -2.025928383 0.000001 GDF11 10220 2.510679113 0.000001 TRIB1 10221 -3.174697119 0.000001 SPRY1 10252 2.433443784 0.000001 IGSF6 10261 -4.11807866 0.000001 RTN3 10313 -2.130077441 0.000001 CRISP3 10321 -7.406042377 0.000001 SIRPB1 10326 -4.975924699 0.000001 CITED2 10370 -2.158576574 0.000001 PRG3 10394 -5.814913716 0.000001 BASP1 10409 -4.68874325 0.000001 UBAC1 10422 -2.202087493 0.000001 ATG7 10533 -2.053098131 0.000001 SLC19A2 10560 -3.190095665 0.000001 OLFM4 10562 -6.057067118 0.000001 GNLY 10578 -2.682778324 0.000001 ERLIN1 10613 -3.045159424 0.000001 IVNS1ABP 10625 -2.258928424 0.000001 GAS2L1 10634 -3.504272548 0.000001 IGF2BP3 10643 3.171735743 0.000001 KHDRBS3 10656 2.331015797 0.000001 KLF1 10661 -4.577273322 0.000001 TNFSF13B 10673 -3.691095913 0.000001 YME1L1 10730 -2.044489395 0.000001 STAG3 10734 4.300850139 0.000001 TRAF3IP2 10758 2.945848768 0.000001 ARPP21 10777 4.19671984 0.000001 GJB6 10804 -4.754941619 0.000001 FAXDC2 10826 -3.428206527 0.000001 ADAM28 10863 -2.541652066 0.000001 NMU 10874 -3.231275627 0.000001 FGL2 10875 -4.611551088 0.000001 MTMR11 10903 -2.165648705 0.000001 SMPDL3A 10924 -3.679883801 0.000001 EHD1 10938 -2.313169001 0.000001 PRSS21 10942 -2.92845165 0.000001 MLLT11 10962 3.033468929 0.000001 CKAP4 10970 -4.087661907 0.000001 FERMT2 10979 2.057265343 0.000001 RAB32 10981 -5.846248691 0.000001 251

SLC27A2 11001 -2.866056768 0.000001 LILRA3 11026 -4.87542932 0.000001 RAB31 11031 -5.804230782 0.000001 ABHD2 11057 -2.063546363 0.000001 C10orf10 11067 2.845376816 0.000001 PTP4A3 11156 3.095465213 0.000001 NUDT5 11164 2.286060935 0.000001 IRAK3 11213 -2.052860437 0.000001 PADI2 11240 -3.758340019 0.000001 GPR176 11245 2.014670755 0.000001 KLF8 11279 2.49659419 0.000001 CD300A 11314 -2.096665091 0.000001 MGLL 11343 -2.578820481 0.000001 TFEC 22797 -3.829119996 0.000001 PHLDA1 22822 -2.422309476 0.000001 ENPP4 22875 -3.615990372 0.000001 MLXIP 22877 2.367881726 0.000001 ZHX2 22882 2.856130141 0.000001 DIP2C 22982 2.453311891 0.000001 MAPKBP1 23005 2.982322319 0.000001 KIF21B 23046 -2.345962045 0.000001 PEG10 23089 2.203577823 0.000001 ZNF423 23090 5.752158981 0.000001 SPG20 23111 -2.56970215 0.000001 9/6/2017 23157 2.481449466 0.000001 SLC35D1 23169 2.378214483 0.000001 CEP68 23177 2.725005184 0.000001 RGL1 23179 3.840368122 0.000001 RFTN1 23180 2.326274648 0.000001 MLC1 23209 -2.63864714 0.000001 PHLPP1 23239 2.425282393 0.000001 MTCL1 23255 2.929810896 0.000001 ACSL6 23305 -3.381724251 0.000001 ICOSLG 23308 2.899452447 0.000001 DMXL2 23312 -2.091949156 0.000001 SYNE1 23345 -4.741511205 0.000001 VPS8 23355 -2.050365263 0.000001 PSD3 23362 6.187162538 0.000001 FRAT2 23401 -2.85523095 0.000001 COTL1 23406 -3.27288558 0.000001 ABCB10 23456 -2.45721248 0.000001 ABCA5 23461 -2.580240843 0.000001 MACF1 23499 2.006062933 0.000001 SPIDR 23514 -4.19210933 0.000001 DDAH2 23564 2.466434799 0.000001 252

PADI4 23569 -4.696303112 0.000001 CLEC5A 23601 -5.107005283 0.000001 CORO1C 23603 -2.484818852 0.000001 SSBP2 23635 3.592255047 0.000001 SSBP3 23648 -2.361572803 0.000001 GABARAPL1 23710 -2.49234875 0.000001 OSBP2 23762 -2.212594934 0.000001 IL17RA 23765 -2.296256296 0.000001 QPCT 25797 -5.414637258 0.000001 GCA 25801 -4.504479489 0.000001 TNFAIP8 25816 -2.081549693 0.000001 NOCT 25819 -2.452595618 0.000001 TMEM158 25907 -2.127036818 0.000001 MYRIP 25924 2.708898706 0.000001 CNRIP1 25927 -4.667371038 0.000001 SAMHD1 25939 -2.231790333 0.000001 PNKD 25953 -2.574508036 0.000001 PPP1R16B 26051 2.451973182 0.000001 AUTS2 26053 3.782747404 0.000001 GPSM1 26086 3.828780163 0.000001 TANC2 26115 -2.473190284 0.000001 FGFR1OP2 26127 -2.259313082 0.000001 TES 26136 -2.934948066 0.000001 PTPN22 26191 -2.872908285 0.000001 STAP1 26228 2.118059453 0.000001 CLEC4E 26253 -2.709087867 0.000001 PLEK2 26499 -2.393235723 0.000001 PCOLCE2 26577 -5.04890218 0.000001 GREM1 26585 2.297405627 0.000001 GPR160 26996 -4.162145259 0.000001 BEX3 27018 -4.096492651 0.000001 SIGLEC7 27036 -2.792164571 0.000001 LAT 27040 -2.506210354 0.000001 AHDC1 27245 3.287956512 0.000001 STK39 27347 2.259535034 0.000001 SLCO3A1 28232 -2.591864195 0.000001 IGHV5-78 28387 2.427540839 0.000001 DLL1 28514 2.811132364 0.000001 TMEM176B 28959 -2.737735202 0.000001 RGCC 28984 -2.793691775 0.000001 LGALSL 29094 -4.498080742 0.000001 CNIH4 29097 -2.172283855 0.000001 UHRF1 29128 2.653546371 0.000001 BLNK 29760 4.900857478 0.000001 PARVB 29780 -2.704113248 0.000001 253

YPEL1 29799 2.679046963 0.000001 VPREB3 29802 5.028716026 0.000001 SNX10 29887 -4.171926567 0.000001 PILRA 29992 -3.251808844 0.000001 SLC40A1 30061 -5.343468696 0.000001 G0S2 50486 -4.946226646 0.000001 CLEC4A 50856 -2.639500436 0.000001 HEBP1 50865 -2.186458252 0.000001 ABHD5 51099 -3.4367452 0.000001 ABHD17B 51104 2.535558576 0.000001 ZNF706 51123 2.09793685 0.000001 EGFL7 51162 2.423909291 0.000001 LEF1 51176 3.64804363 0.000001 TCEAL9 51186 -2.594366639 0.000001 ACP6 51205 -2.242120551 0.000001 CRIM1 51232 3.941780888 0.000001 MZB1 51237 2.984603302 0.000001 3/2/2017 51257 -2.126390915 0.000001 TMEM216 51259 -3.232035692 0.000001 CLEC1B 51266 -2.655636748 0.000001 SLC15A3 51296 -3.005177479 0.000001 NRN1 51299 3.123952695 0.000001 ZDHHC3 51304 -2.426513354 0.000001 TLR8 51311 -4.47616386 0.000001 SLC25A37 51312 -3.656718908 0.000001 FAM198B 51313 -4.121999249 0.000001 NME8 51314 -3.629247354 0.000001 AHSP 51327 -4.570463976 0.000001 MS4A4A 51338 -2.012228495 0.000001 KLRF1 51348 -2.106784951 0.000001 ZNF117 51351 2.712951211 0.000001 HOOK1 51361 -2.148681872 0.000001 CHST15 51363 2.346227868 0.000001 AIG1 51390 -4.458553516 0.000001 BIN2 51411 -3.927144993 0.000001 ZNF107 51427 2.55994264 0.000001 TRMT6 51605 -2.044138007 0.000001 SLC25A39 51629 -2.163213583 0.000001 DHRS7 51635 -2.537792847 0.000001 RASD1 51655 3.995099003 0.000001 MSRB1 51734 -4.309521651 0.000001 GALNT7 51809 2.308886674 0.000001 CECR1 51816 -2.774971867 0.000001 BCL11A 53335 3.255448877 0.000001 TM6SF1 53346 -4.317776868 0.000001 254

P2RY13 53829 -5.427325994 0.000001 GPR84 53831 -4.467554383 0.000001 PELO 53918 -2.019109528 0.000001 H2BFS 54145 2.080750379 0.000001 TREM1 54210 -5.015430103 0.000001 SH3TC1 54436 2.899039468 0.000001 ANLN 54443 -2.037856355 0.000001 FAM105A 54491 -2.994067726 0.000001 NEURL1B 54492 2.142618868 0.000001 RBM47 54502 -2.325369792 0.000001 CPVL 54504 -4.41467228 0.000001 SDK2 54549 4.14484401 0.000001 CRLS1 54675 -2.108759114 0.000001 MANSC1 54682 -3.929082416 0.000001 TET2 54790 -2.088492721 0.000001 KLHL24 54800 2.265901635 0.000001 FAM46C 54855 -3.504421517 0.000001 LPCAT2 54947 -2.651240735 0.000001 TESC 54997 -4.209119226 0.000001 3/1/2017 55016 -2.477322729 0.000001 BANK1 55024 3.19592945 0.000001 VPS37C 55048 -2.166882092 0.000001 FLJ10038 55056 2.203423772 0.000001 WIPI1 55062 -3.798225608 0.000001 ANO10 55129 -2.944841508 0.000001 FAIM 55179 2.086123849 0.000001 DPPA4 55211 -2.635363024 0.000001 STEAP3 55240 -3.79054632 0.000001 QRSL1 55278 2.572584119 0.000001 CPPED1 55313 -2.679530933 0.000001 STRBP 55342 3.274387823 0.000001 VNN3 55350 -3.957831587 0.000001 STK32B 55351 5.151912209 0.000001 LAPTM4B 55353 -2.296901794 0.000001 SLC22A15 55356 -5.738491309 0.000001 HEMGN 55363 -4.306767111 0.000001 STRADB 55437 -2.489053093 0.000001 DNAJA4 55466 -2.873856688 0.000001 SLC35E3 55508 2.492315429 0.000001 TMEM55A 55529 -3.425874505 0.000001 SOX6 55553 -3.31852028 0.000001 FAM46A 55603 -5.486139222 0.000001 KIF16B 55614 2.174262067 0.000001 RAB20 55647 -2.102847062 0.000001 BCAS4 55653 3.000458318 0.000001 255

FAR2 55711 -2.86427959 0.000001 ENAH 55740 3.385694857 0.000001 UGGT2 55757 -2.510415918 0.000001 C1orf106 55765 -2.059592818 0.000001 CACNA2D3 55799 -2.387373004 0.000001 PECR 55825 -2.5739496 0.000001 BEX1 55859 -5.888397095 0.000001 ZC4H2 55906 2.029449802 0.000001 APOBR 55911 -3.297061203 0.000001 MYO5C 55930 4.564428286 0.000001 NXF3 56000 -2.088673522 0.000001 PDGFC 56034 -4.103424068 0.000001 GPCPD1 56261 -2.138723189 0.000001 PARD3 56288 2.674648854 0.000001 ERO1B 56605 2.41496135 0.000001 KCNK12 56660 3.520032982 0.000001 SUCNR1 56670 -3.401132266 0.000001 RETN 56729 -5.016271938 0.000001 MAP3K7CL 56911 -2.028640967 0.000001 SPPL2B 56928 2.042695843 0.000001 HMCES 56941 2.487721836 0.000001 STOX2 56977 -2.112014315 0.000001 AGTRAP 57085 -3.655077091 0.000001 CD177 57126 -4.720032553 0.000001 APMAP 57136 -2.933478536 0.000001 PELI1 57162 -2.155434935 0.000001 ATP8B2 57198 2.314062616 0.000001 SLC45A4 57210 -2.055887304 0.000001 TTC7A 57217 2.100595652 0.000001 ERGIC1 57222 2.318036739 0.000001 SMAGP 57228 3.428143996 0.000001 KIAA0485 57235 -2.082086665 0.000001 TMCC3 57458 -2.451725743 0.000001 PLEKHG1 57480 2.487508492 0.000001 KIAA1211 57482 -2.498384559 0.000001 RIMKLB 57494 2.360567315 0.000001 ZNF608 57507 4.121750703 0.000001 NCEH1 57552 -3.232842309 0.000001 SEMA6A 57556 2.778860556 0.000001 CRAMP1 57585 2.126179204 0.000001 BAHCC1 57597 2.686312796 0.000001 SH3RF1 57630 2.847867347 0.000001 FBRSL1 57666 2.086794257 0.000001 SHTN1 57698 -3.017945693 0.000001 TRIB3 57761 -3.121102682 0.000001 256

SLAMF7 57823 -2.538932408 0.000001 HMHB1 57824 4.462488213 0.000001 CXCL16 58191 -2.609836818 0.000001 SQRDL 58472 -3.080833792 0.000001 MS4A7 58475 -2.929205171 0.000001 RHOU 58480 -2.954013257 0.000001 NLRC4 58484 -3.705373983 0.000001 RRAGD 58528 -2.25686898 0.000001 BACH2 60468 2.526888118 0.000001 PROK2 60675 -4.973584058 0.000001 GALNT11 63917 -3.0059322 0.000001 SLC28A3 64078 -3.705470959 0.000001 SAMSN1 64092 -2.986820916 0.000001 LRRC4 64101 -2.830285064 0.000001 C10orf54 64115 -2.576147966 0.000001 NOD2 64127 -3.130207899 0.000001 DPEP2 64174 -2.147002369 0.000001 SEMA4A 64218 -3.045531517 0.000001 MS4A6A 64231 -3.478511756 0.000001 MMP25 64386 -3.046934491 0.000001 ZMAT3 64393 2.723560288 0.000001 AKTIP 64400 -2.681671738 0.000001 RGS18 64407 -4.469021913 0.000001 ARAP3 64411 -2.028220858 0.000001 NARFL 64428 2.005810699 0.000001 CLEC7A 64581 -4.994271464 0.000001 3/1/2017 64757 -4.797701372 0.000001 FNDC3B 64778 -3.886900375 0.000001 LMF1 64788 2.130996312 0.000001 BCL11B 64919 -2.017830804 0.000001 MARCKSL1 65108 2.088637235 0.000001 INTS3 65123 2.159914862 0.000001 ZBTB10 65986 2.179606905 0.000001 C11orf95 65998 2.783548078 0.000001 TRAK2 66008 -2.041212038 0.000001 PRRG4 79056 -4.326874312 0.000001 MMP28 79148 2.22227249 0.000001 IRX1 79192 3.001782323 0.000001 BEND5 79656 2.808280248 0.000001 VEPH1 79674 -2.041870996 0.000001 STEAP4 79689 -3.083161336 0.000001 MCTP1 79772 -2.532524881 0.000001 MICALL2 79778 -2.708131401 0.000001 NIPAL2 79815 -2.266752079 0.000001 MOB3B 79817 2.52343149 0.000001 257

PIP4K2C 79837 -3.115067852 0.000001 BAALC 79870 2.917883906 0.000001 RABEP2 79874 2.933208567 0.000001 PLBD1 79887 -6.981533868 0.000001 ATP8B4 79895 -4.719508713 0.000001 CYBRD1 79901 -3.571449422 0.000001 SCD5 79966 2.538460734 0.000001 WLS 79971 -2.023224797 0.000001 DOCK5 80005 -4.501406725 0.000001 TMEM156 80008 2.15432896 0.000001 ASRGL1 80150 -3.542095643 0.000001 RAB11FIP1 80223 -2.984593691 0.000001 PLEKHO2 80301 -2.100010399 0.000001 CPEB4 80315 -2.240863292 0.000001 NDFIP1 80762 -3.046067241 0.000001 SETD7 80854 2.204979104 0.000001 LNPK 80856 -2.258728045 0.000001 NPL 80896 -3.671922636 0.000001 TUBB1 81027 -5.741726753 0.000001 ZBP1 81030 -2.187815499 0.000001 SLC38A1 81539 2.22764684 0.000001 FAM83D 81610 -2.301475331 0.000001 ARHGAP24 83478 -2.125183441 0.000001 SOX7 83595 2.98951208 0.000001 CALN1 83698 2.408786961 0.000001 CRISPLD2 83716 -4.454738477 0.000001 ROPN1L 83853 -2.829969106 0.000001 FGFBP2 83888 -2.227022454 0.000001 C10orf11 83938 -3.527527493 0.000001 KBTBD7 84078 -2.381966228 0.000001 PRAM1 84106 -4.283581554 0.000001 ARID5B 84159 2.204345071 0.000001 ZCCHC7 84186 4.451519214 0.000001 LRRC8C 84230 2.667786083 0.000001 C2orf88 84281 -3.168652517 0.000001 FAM213A 84293 2.719273909 0.000001 CYSTM1 84418 -4.240979248 0.000001 C15orf48 84419 -2.251499272 0.000001 NTNG2 84628 -2.322246216 0.000001 LOXL3 84695 -2.422103713 0.000001 BEX2 84707 -3.423414977 0.000001 GPAT3 84803 -4.652252342 0.000001 POMGNT2 84892 -2.220670506 0.000001 PLXDC2 84898 -2.105598693 0.000001 DIRC2 84925 -2.384615575 0.000001 258

UBASH3B 84959 3.402108372 0.000001 HIST1H2BK 85236 2.120381594 0.000001 COL27A1 85301 2.510801863 0.000001 LGALS12 85329 -3.612770979 0.000001 SHANK3 85358 4.798806468 0.000001 FHDC1 85462 -2.922117112 0.000001 HPS4 89781 2.790443692 0.000001 SIGLEC10 89790 -2.067629105 0.000001 NAV1 89796 4.319883401 0.000001 KLHL6 89857 2.4851372 0.000001 SLC25A21 89874 -3.108541773 0.000001 KBTBD6 89890 -2.811617909 0.000001 CEACAM21 90273 -2.170447957 0.000001 TMEM263 90488 3.40546017 0.000001 SEC11C 90701 -2.202506784 0.000001 C12orf29 91298 -2.773435505 0.000001 NLRP12 91662 -2.525791745 0.000001 PXYLP1 92370 -2.234266636 0.000001 DTD1 92675 -3.37102222 0.000001 HIST3H2A 92815 -2.140959758 0.000001 HNRNPLL 92906 -2.540235902 0.000001 ATPIF1 93974 -2.227520261 0.000001 BMS1P20 96610 -2.386734752 0.000001 CMTM7 112616 2.063837247 0.000001 GLMP 112770 -2.467608459 0.000001 TMEM106A 113277 3.153125408 0.000001 LACTB 114294 -2.481762504 0.000001 NLRP3 114548 -2.548015186 0.000001 ERMAP 114625 -2.629684639 0.000001 CYGB 114757 4.275506854 0.000001 CARD16 114769 -2.582525011 0.000001 FMNL2 114793 3.097222238 0.000001 ELFN2 114794 2.661579856 0.000001 OSBPL1A 114876 -2.4290168 0.000001 C1QTNF4 114900 2.177439545 0.000001 ZNF618 114991 2.42813649 0.000001 KCTD12 115207 -2.709955079 0.000001 DDIT4L 115265 2.981392435 0.000001 GBP4 115361 2.633631928 0.000001 CTHRC1 115908 3.200537367 0.000001 FAM210B 116151 -2.986610784 0.000001 RBP7 116362 -5.364746215 0.000001 SLC26A8 116369 -2.339181842 0.000001 FAM129A 116496 -2.249416521 0.000001 LRG1 116844 -3.3902657 0.000001 259

THEM4 117145 -2.457261236 0.000001 ANKRD22 118932 -3.47047878 0.000001 PDZD8 118987 -2.919839907 0.000001 SLC36A4 120103 -2.902427129 0.000001 JAML 120425 -3.281734581 0.000001 LRRK2 120892 -4.341712182 0.000001 TMEM52B 120939 -2.598570168 0.000001 FGD4 121512 -2.730008377 0.000001 JDP2 122953 -2.764101192 0.000001 TTC8 123016 -3.500569695 0.000001 SLC24A4 123041 -3.03900761 0.000001 ZG16B 124220 -2.905990663 0.000001 MSI2 124540 3.186135062 0.000001 TBC1D16 125058 2.775476247 0.000001 INO80C 125476 3.059715844 0.000001 FAM69C 125704 2.610661514 0.000001 OSCAR 126014 -3.910489899 0.000001 ZNF57 126295 2.849202726 0.000001 LRRC25 126364 -3.068265503 0.000001 C1orf162 128346 -3.749192476 0.000001 MBOAT2 129642 -3.299887098 0.000001 PPM1M 132160 -2.274667402 0.000001 CPEB2 132864 -2.082498061 0.000001 STXBP5 134957 -3.549430854 0.000001 CD109 135228 3.11484802 0.000001 TMEM71 137835 -3.216884764 0.000001 UBXN2B 137886 -2.458615401 0.000001 NACC2 138151 -3.56697804 0.000001 FAM69B 138311 3.789158819 0.000001 MYL6B 140465 2.410453205 0.000001 S100A16 140576 4.610645201 0.000001 MACROD2 140733 4.919057758 0.000001 SRXN1 140809 -3.093606396 0.000001 FAM65C 140876 -3.813472114 0.000001 SIRPA 140885 -3.492349422 0.000001 MPP7 143098 -3.263027368 0.000001 HECTD2 143279 2.093658814 0.000001 SESN3 143686 -2.33459782 0.000001 PIWIL4 143689 -2.828512774 0.000001 GLT1D1 144423 -3.795465143 0.000001 SOCS2-AS1 144481 4.873849543 0.000001 TMED6 146456 3.41332228 0.000001 CD300LF 146722 -3.418389028 0.000001 DHRS13 147015 -2.2697974 0.000001 APCDD1 147495 2.92672305 0.000001 260

ZNF738 148203 2.050348457 0.000001 CREB3L4 148327 2.008131214 0.000001 TMEM56 148534 -4.863091559 0.000001 ABCC13 150000 -2.046763879 0.000001 NFAM1 150372 -3.563388121 0.000001 FBXO41 150726 2.289760405 0.000001 GLIPR2 152007 -3.842037444 0.000001 CCDC50 152137 2.283735493 0.000001 CMTM8 152189 4.19122779 0.000001 PLEKHG4B 153478 3.418811091 0.000001 BTNL9 153579 2.945815783 0.000001 RNF217 154214 -2.503043299 0.000001 ABCA13 154664 -2.846663258 0.000001 SGK223 157285 2.80238308 0.000001 LCN6 158062 2.179335917 0.000001 FAM201A 158228 -2.182818931 0.000001 CLEC12A 160364 -5.373693982 0.000001 DGKH 160851 2.00117683 0.000001 CLEC14A 161198 3.407185136 0.000001 ZFPM1 161882 -2.063472629 0.000001 ZNF709 163051 3.023560817 0.000001 PARP15 165631 2.069094631 0.000001 ADGRA3 166647 2.258415859 0.000001 SGMS2 166929 -3.50007523 0.000001 MPZL3 196264 -3.110854801 0.000001 PLBD2 196463 -2.373060777 0.000001 DZIP1L 199221 -2.202862308 0.000001 MCEMP1 199675 -4.395617831 0.000001 FAM129C 199786 3.670972133 0.000001 SLC39A11 201266 -2.387132612 0.000001 SPNS3 201305 -2.138688463 0.000001 TMEM154 201799 -2.471447411 0.000001 TAPT1-AS1 202020 2.733834533 0.000001 LOC202181 202181 2.07018046 0.000001 CCDC125 202243 -2.417774971 0.000001 TMEM268 203197 2.793646337 0.000001 SLC36A1 206358 -3.158996188 0.000001 YPEL4 219539 -2.96452452 0.000001 MPEG1 219972 -4.587181125 0.000001 PATL1 219988 -2.041247096 0.000001 FAM171A1 221061 2.066129112 0.000001 RNF182 221687 -2.990493012 0.000001 JAZF1 221895 -3.074872518 0.000001 ADGRG3 222487 -3.928994832 0.000001 TSPO2 222642 -3.743178063 0.000001 261

ZBTB38 253461 -2.517938461 0.000001 MSRB3 253827 -4.080243664 0.000001 SLC41A1 254428 2.490883293 0.000001 RNF144B 255488 -3.495413679 0.000001 NATD1 256302 -2.633580607 0.000001 FRMD3 257019 -2.534820844 0.000001 NEGR1 257194 2.261367337 0.000001 SGMS1 259230 -2.549668404 0.000001 RGL4 266747 -4.731137179 0.000001 C17orf58 284018 -2.8906698 0.000001 VSTM1 284415 -5.360152877 0.000001 SEC14L4 284904 -3.537651224 0.000001 FAM150B 285016 3.470400781 0.000001 LOC285097 285097 3.398494894 0.000001 ARL10 285598 2.598057184 0.000001 LINC01268 285758 -2.954558233 0.000001 TRIQK 286144 -2.935697945 0.000001 SCAI 286205 2.183901311 0.000001 EIF4E3 317649 -3.330943398 0.000001 RFESD 317671 -3.341066495 0.000001 CLEC4D 338339 -4.70351261 0.000001 ZC3H12D 340152 3.210682878 0.000001 PAQR9 344838 -2.92985525 0.000001 NMNAT3 349565 -2.135047323 0.000001 SLCO4C1 353189 -3.556892162 0.000001 LILRA5 353514 -3.24858399 0.000001 RFLNB 359845 -2.106852328 0.000001 B3GNT8 374907 -2.201968974 0.000001 RAB15 376267 2.056902984 0.000001 SH2D4B 387694 2.26757897 0.000001 SHISA2 387914 2.437139635 0.000001 LRRC75A 388341 -2.424945821 0.000001 SMIM1 388588 -2.689559771 0.000001 ARHGEF37 389337 -2.394088518 0.000001 NUDT19 390916 -2.489654583 0.000001 LINC00642 400238 2.718918703 0.000001 LCN10 414332 2.994577531 0.000001 MXRA7 439921 2.22800022 0.000001 BCL2L15 440603 -2.951781029 0.000001 C5orf56 441108 3.142861704 0.000001 CHAC2 494143 -2.03332371 0.000001 ZNF704 619279 3.549638158 0.000001 LEF1-AS1 641518 4.021418565 0.000001 ECRP 643332 -5.079633489 0.000001 LINC00865 643529 3.133389454 0.000001 262

HHIP-AS1 646576 2.807472873 0.000001 LOC728175 728175 2.061102061 0.000001 CCR2 729230 -2.639692376 0.000001 H2BFXP 767811 2.859246652 0.000001 HAR1A 768096 2.268445793 0.000001 PRCD 768206 2.858646068 0.000001 TMEM170B 1E+08 -3.848130367 0.000001 C8orf88 1E+08 -2.240169772 0.000001 GABPB1-AS1 1E+08 2.736432527 0.000001 DPY19L1P1 1E+08 -2.796273933 0.000001 LOC10013045 8 1E+08 5.040504761 0.000001 LOC10013047 6 1E+08 2.199619374 0.000001 WASIR2 1E+08 2.431220117 0.000001 C17orf99 1E+08 -3.775277384 0.000001 HBD 1E+08 -3.364828056 0.000001 PAN3-AS1 1E+08 3.436176309 0.000001 ERVH-3 1E+08 2.871838721 0.000001 ANKRD10- 1.01E+0 IT1 8 2.114551238 0.000001 LOC10050550 1.01E+0 1 8 2.814511511 0.000001 LOC10050556 1.01E+0 4 8 2.773189118 0.000001 1.01E+0 ITGB2-AS1 8 -2.687431055 0.000001 1.01E+0 LINC01013 8 5.054765057 0.000001 LOC10106039 1.01E+0 1 8 2.22528121 0.000001 1.02E+0 GBAT2 8 2.780724998 0.000001 1.02E+0 UBXN10-AS1 8 2.020298457 0.000001 LOC10192804 1.02E+0 7 8 -2.256405514 0.000001 LOC10192842 1.02E+0 9 8 -2.83988207 0.000001 1.02E+0 HSPC324 8 2.348160797 0.000001 LOC10192884 1.02E+0 1 8 2.472588464 0.000001 1.02E+0 LINC01215 8 3.005534677 0.000001 LOC10537727 1.05E+0 6 8 -2.079535633 0.000001

263

Appendix O

Master List of DEGs in T-ALL

264

DEGs from the T-ALL subset

Entrez Log Fold- Adjusted p- Gene Symbol ID change value

ACPP 55 -3.654711885 0.000001 ACTN1 87 -2.55141432 0.000001 ACVR2B 93 2.64654248 0.000001 ADA 100 3.966057942 0.000001 ADAM8 101 -2.912941504 0.000001 ADD2 119 -5.115064263 0.000001 ADM 133 -2.609054546 0.000001 ADRB2 154 -3.494758715 0.000001 GRK3 157 2.192736651 0.000001 AEBP1 165 3.673966142 0.000001 JAG1 182 -3.779373354 0.000001 AK1 203 -2.244264644 0.000001 ALAD 210 -2.711190396 0.000001 ALAS1 211 -2.284794202 0.000001 ALAS2 212 -5.207530771 0.000001 ALDH1A1 216 -3.425192188 0.000001 ALDH2 217 -2.858678842 0.000001 ALDH3B1 221 -4.101571435 0.000001 ALDH3A2 224 -3.050641437 0.000001 ALOX12 239 -2.104049837 0.000001 ALOX5 240 -4.083028987 0.000001 ALOX5AP 241 -3.562722369 0.000001 AMPD3 272 -2.422614455 0.000001 ANK1 286 -4.074470121 0.000001 ANPEP 290 -3.184769986 0.000001 ANXA1 301 -2.26163981 0.000001 ANXA3 306 -5.596898707 0.000001 ANXA4 307 -2.769981113 0.000001 ANXA5 308 -3.227760618 0.000001 AOAH 313 -2.239855857 0.000001 APLP2 334 -2.569956422 0.000001 APOC1 341 -3.28312871 0.000001 APP 351 -3.804188474 0.000001 FAS 355 -2.711825334 0.000001 AQP1 358 -4.833541763 0.000001 AQP9 366 -3.168465052 0.000001 AREG 374 -3.198016419 0.000001 ARG1 383 -5.372643194 0.000001 RHOB 388 -2.335644278 0.000001 RHOH 399 2.670724077 0.000001 265

ARSD 414 -2.165618552 0.000001 ASAH1 427 -3.111025652 0.000001 ASL 435 -2.593768726 0.000001 FXYD2 486 3.606267619 0.000001 ATP6V1C1 528 -2.155638537 0.000001 ATP6V0A1 535 -2.236855213 0.000001 AZU1 566 -6.794952315 0.000001 BCL2A1 597 -3.331056991 0.000001 BCL7A 605 2.326128293 0.000001 CEACAM1 634 -4.425661833 0.000001 BICD1 636 2.250121499 0.000001 BLVRA 644 -2.313527271 0.000001 BLVRB 645 -4.289532036 0.000001 BMX 660 -2.650472937 0.000001 BPGM 669 -2.467436246 0.000001 BSG 682 -2.32284569 0.000001 BST1 683 -4.378841099 0.000001 KLF9 687 -3.081629774 0.000001 KLF5 688 -3.699256145 0.000001 BTK 695 -2.457509997 0.000001 TSPO 706 -3.332842687 0.000001 C1QB 713 -2.814039271 0.000001 C1QC 714 -2.822219209 0.000001 C3AR1 719 -3.710280671 0.000001 C5AR1 728 -4.408030263 0.000001 LDLRAD4 753 2.830303762 0.000001 CA1 759 -4.149206951 0.000001 CA2 760 -4.977370604 0.000001 CA3 761 -2.94437502 0.000001 CA4 762 -2.655631592 0.000001 CACNB3 784 3.34808209 0.000001 CALD1 800 -2.235060588 0.000001 CAMK4 814 3.349697468 0.000001 CAMP 820 -6.506226445 0.000001 CAPN2 824 -2.121693549 0.000001 CAPN3 825 -3.23026683 0.000001 CAST 831 -3.058406068 0.000001 CAT 847 -2.872247368 0.000001 CBFA2T3 863 2.591862712 0.000001 CCND2 894 -2.064393552 0.000001 CD1A 909 3.45813546 0.000001 CD1B 910 4.638868584 0.000001 CD1E 913 4.892323707 0.000001 CD3D 915 4.015425771 0.000001 CD3E 916 2.898621187 0.000001 266

CD3G 917 3.925795796 0.000001 CD7 924 3.239651584 0.000001 CD9 928 -2.897098709 0.000001 CD14 929 -4.477839335 0.000001 MS4A1 931 -2.144574367 0.000001 MS4A3 932 -6.397669622 0.000001 CD86 942 -3.263329429 0.000001 CD33 945 -3.711045113 0.000001 CD36 948 -5.107463368 0.000001 ENTPD1 953 -3.54213231 0.000001 CD58 965 -2.326142757 0.000001 CD59 966 -4.093780346 0.000001 CD63 967 -2.311437076 0.000001 CD74 972 -2.427388158 0.000001 CDA 978 -5.520674086 0.000001 CDH1 999 -3.744906967 0.000001 CDH2 1000 2.921610123 0.000001 CDK6 1021 3.841712499 0.000001 CDKN3 1033 -2.002435096 0.000001 CEBPA 1050 -3.574157741 0.000001 CEBPB 1051 -2.112761692 0.000001 CEBPD 1052 -3.73310899 0.000001 CEBPE 1053 -3.429273636 0.000001 CEL 1056 2.636377906 0.000001 CES1 1066 -3.77087832 0.000001 CEACAM3 1084 -3.139810957 0.000001 CEACAM8 1088 -6.210526564 0.000001 RCBTB2 1102 2.966054867 0.000001 CHI3L1 1116 -5.497323157 0.000001 CHI3L2 1117 5.381683322 0.000001 CHIT1 1118 -4.609870663 0.000001 LYST 1130 -2.891185857 0.000001 CHRNA3 1136 3.179606313 0.000001 CLC 1178 -5.395883188 0.000001 CLCN4 1183 -2.402763829 0.000001 CLU 1191 -4.134792453 0.000001 CLIC2 1193 -2.861547094 0.000001 TPP1 1200 -2.038663817 0.000001 CCR1 1230 -3.93494723 0.000001 CCR7 1236 -2.022920469 0.000001 LTB4R 1241 -2.08040284 0.000001 CNN2 1265 -2.155675776 0.000001 COL1A1 1277 -2.096474393 0.000001 COL17A1 1308 -3.905996091 0.000001 SLC31A1 1317 -2.034578109 0.000001 267

SLC31A2 1318 -3.109158084 0.000001 MAP3K8 1326 -2.264876068 0.000001 CPA3 1359 -3.51305253 0.000001 CPD 1362 -2.432071006 0.000001 CPM 1368 -2.168733377 0.000001 CPOX 1371 -2.702438966 0.000001 CR1L 1379 -3.948676972 0.000001 CR2 1380 2.913722635 0.000001 CREBL2 1389 -2.161823265 0.000001 CSF2RA 1438 -2.448854992 0.000001 CSF2RB 1439 -4.137645721 0.000001 CSF3R 1441 -3.285411101 0.000001 VCAN 1462 -3.762194648 0.000001 CST3 1471 -3.402046798 0.000001 CSTA 1475 -4.661564657 0.000001 CTBP2 1488 -2.403985291 0.000001 CTNNA1 1495 -3.120236626 0.000001 CTSB 1508 -2.800108999 0.000001 CTSD 1509 -2.353531199 0.000001 CTSE 1510 -3.74623493 0.000001 CTSG 1511 -5.859791464 0.000001 CTSH 1512 -2.821873585 0.000001 CTSL 1514 -3.187741328 0.000001 CTSS 1520 -3.04289954 0.000001 CTSZ 1522 -2.806214641 0.000001 CX3CR1 1524 -2.022484235 0.000001 CYBA 1535 -2.28518311 0.000001 CYBB 1536 -4.584216128 0.000001 CYP1B1 1545 -3.444144703 0.000001 DACH1 1602 -2.349498276 0.000001 CD55 1604 -2.270040581 0.000001 DBN1 1627 3.662342928 0.000001 DEFA4 1669 -6.11277832 0.000001 DEFB1 1672 -2.252403322 0.000001 CFD 1675 -3.516620471 0.000001 9/1/2017 1731 2.391418891 0.000001 DNMT3A 1788 2.222482997 0.000001 DNMT3B 1789 2.062825073 0.000001 DNTT 1791 4.539816702 0.000001 DSG2 1829 2.086051927 0.000001 HBEGF 1839 -2.452786503 0.000001 DUSP6 1848 -2.181590992 0.000001 GPR183 1880 -2.887961215 0.000001 TYMP 1890 -2.551391325 0.000001 PHC1 1911 2.458179626 0.000001 268

MEGF9 1955 -2.375267068 0.000001 EGR1 1958 -2.246792948 0.000001 ELANE 1991 -6.125902672 0.000001 SERPINB1 1992 -2.07173608 0.000001 CTTN 2017 -3.05474168 0.000001 EPB42 2038 -4.986551176 0.000001 DMTN 2039 -3.33462234 0.000001 STOM 2040 -4.740489036 0.000001 EPHB6 2051 3.212509038 0.000001 EPOR 2057 -3.212438547 0.000001 EREG 2069 -4.167333 0.000001 F5 2153 -3.324126131 0.000001 F13A1 2162 -3.950268623 0.000001 FABP4 2167 -2.621815677 0.000001 ACSL1 2180 -3.950275233 0.000001 FAT1 2195 3.996820902 0.000001 FBN2 2201 -3.628606965 0.000001 FBP1 2203 -3.352355978 0.000001 FCAR 2204 -4.459035663 0.000001 FCER1A 2205 -3.237586287 0.000001 FCER1G 2207 -4.223730694 0.000001 FCGR1B 2210 -4.009469391 0.000001 FCGR2A 2212 -3.478115898 0.000001 FCGR3B 2215 -3.367577958 0.000001 FCGRT 2217 -2.22177315 0.000001 FCN1 2219 -4.770934748 0.000001 FECH 2235 -2.903952035 0.000001 FES 2242 -2.913418382 0.000001 FGF13 2258 -3.540778279 0.000001 FGFR1 2260 2.693387086 0.000001 FGR 2268 -3.5685818 0.000001 FHL2 2274 -2.964359845 0.000001 FOXO1 2308 -2.424799208 0.000001 FLOT2 2319 -2.335221782 0.000001 FOLR3 2352 -4.494209025 0.000001 FOS 2353 -2.404134474 0.000001 FOSL2 2355 -2.612738295 0.000001 FPR1 2357 -4.576338092 0.000001 FPR2 2358 -3.105842239 0.000001 FXN 2395 -2.686365063 0.000001 FUCA2 2519 -3.153682956 0.000001 FUT4 2526 -2.17566158 0.000001 ACKR1 2532 -2.934770505 0.000001 G6PD 2539 -2.072185658 0.000001 GALC 2581 -2.386349417 0.000001 269

GALNS 2588 -2.434300628 0.000001 GALNT2 2590 2.940680129 0.000001 GAS2 2620 2.025919395 0.000001 GATA1 2623 -2.266791499 0.000001 GATA3 2625 2.996598588 0.000001 GCNT2 2651 -2.530573243 0.000001 GGTA1P 2681 -5.242574673 0.000001 GCLM 2730 -2.096462065 0.000001 GMPR 2766 -3.682739256 0.000001 GNA15 2769 2.26022036 0.000001 GNG7 2788 -2.707397495 0.000001 GNG11 2791 -3.472928108 0.000001 GNS 2799 -3.125098264 0.000001 GPR27 2850 -4.481232794 0.000001 GPX3 2878 -2.497271472 0.000001 GRB10 2887 -2.087565419 0.000001 GRN 2896 -3.591190139 0.000001 CXCL1 2919 -3.706304132 0.000001 GSN 2934 -4.473692721 0.000001 GTF3A 2971 2.193629911 0.000001 GUCY1A3 2982 3.089750179 0.000001 GYG1 2992 -3.15694041 0.000001 GYPA 2993 -4.386392665 0.000001 GYPB 2994 -4.436031425 0.000001 GYPE 2996 -2.658005739 0.000001 GYS1 2997 -2.193020852 0.000001 GZMH 2999 -2.304126302 0.000001 GZMA 3001 -2.219412405 0.000001 GZMB 3002 -2.503999541 0.000001 GZMK 3003 -2.560195322 0.000001 H1F0 3005 -2.071002475 0.000001 HAGH 3029 -2.18850775 0.000001 HAL 3034 -3.638550525 0.000001 HBM 3042 -4.282068174 0.000001 HBQ1 3049 -3.254018166 0.000001 HCK 3055 -3.911599427 0.000001 HELLS 3070 2.055044182 0.000001 HK3 3101 -4.586829206 0.000001 HLA-DMA 3108 -3.372616934 0.000001 HLA-DMB 3109 -2.274227268 0.000001 HLA-DPA1 3113 -3.126531623 0.000001 HLA-DPB1 3115 -3.052169771 0.000001 HLA-DQB1 3119 -3.12785905 0.000001 HLA-DRA 3122 -3.516148269 0.000001 HLA-DRB4 3126 -2.36849105 0.000001 270

HLA-DRB6 3128 -2.020429019 0.000001 HLX 3142 -4.539584387 0.000001 HMBS 3145 -2.793559755 0.000001 HMOX1 3162 -3.702008661 0.000001 HNMT 3176 -4.09450771 0.000001 HP 3240 -5.12968213 0.000001 HES1 3280 2.860819473 0.000001 HSPA6 3310 -2.445250242 0.000001 ICA1 3382 -2.440125308 0.000001 ICAM1 3383 -2.30624613 0.000001 ICAM4 3386 -2.047670657 0.000001 IRF8 3394 -2.147304906 0.000001 ID2 3398 -2.507565966 0.000001 IFNGR1 3459 -2.107700043 0.000001 IGFBP7 3490 -3.412583057 0.000001 IGHD 3495 -2.457102976 0.000001 IGHM 3507 -2.861853591 0.000001 JCHAIN 3512 -3.716787923 0.000001 IGKC 3514 -4.289027178 0.000001 IGLC1 3537 -2.901128689 0.000001 IL1B 3553 -2.059147842 0.000001 IL1R1 3554 -3.710167595 0.000001 IL1RN 3557 -4.203852077 0.000001 IL2RB 3560 -2.444013789 0.000001 IL6R 3570 -3.34798755 0.000001 CXCL8 3576 -2.760575476 0.000001 CXCR2 3579 -3.257423596 0.000001 IL10RA 3587 -2.744526875 0.000001 IL13RA1 3597 -2.68912688 0.000001 IL18 3606 -3.223868322 0.000001 IMPA2 3613 -2.504162369 0.000001 INHBA 3624 -3.411214918 0.000001 IRAK2 3656 -2.64689757 0.000001 ITGA2B 3674 -2.46852864 0.000001 ITGA9 3680 -2.604767875 0.000001 ITGAM 3684 -3.7091709 0.000001 ITGB2 3689 -2.812625007 0.000001 ITGB3 3690 -2.207496169 0.000001 ITPR2 3709 2.032049235 0.000001 KCNA3 3738 2.337688216 0.000001 KCNH2 3757 -3.337381583 0.000001 KCNJ15 3772 -3.616352031 0.000001 KCNQ1 3784 -2.128046865 0.000001 KEL 3792 -3.743837198 0.000001 KLRD1 3824 -2.278703371 0.000001 271

AFF3 3899 2.125860943 0.000001 LAMP1 3916 -2.058390887 0.000001 LAMP2 3920 -2.235811766 0.000001 LCK 3932 3.761194833 0.000001 LCN2 3934 -5.876990764 0.000001 LGALS2 3957 -2.105792729 0.000001 LGALS3 3958 -4.227334204 0.000001 LGALS3BP 3959 2.420829753 0.000001 LMNA 4000 -2.684558023 0.000001 LMO2 4005 -2.341436659 0.000001 LRP3 4037 -2.393289575 0.000001 LTA4H 4048 -2.038569814 0.000001 CYP4F3 4051 -5.080243038 0.000001 LTBP1 4052 -2.364593724 0.000001 LTF 4057 -5.282272863 0.000001 LYN 4067 -2.69713575 0.000001 SH2D1A 4068 4.875424932 0.000001 LYZ 4069 -4.817198352 0.000001 TACSTD2 4070 -5.566487406 0.000001 EPCAM 4072 -2.816442045 0.000001 MARCKS 4082 -3.986716533 0.000001 MAF 4094 -2.063778913 0.000001 MAL 4118 2.932570649 0.000001 MAN1A1 4121 -2.148857085 0.000001 MAP1A 4130 3.795102493 0.000001 MBP 4155 -2.098678142 0.000001 MCL1 4170 -2.281812589 0.000001 MEF2C 4208 -2.192660307 0.000001 MAP3K5 4217 -2.268516639 0.000001 MGST1 4257 -5.285409709 0.000001 MGST3 4259 -2.63903791 0.000001 CD99 4267 2.733709412 0.000001 MMP8 4317 -6.477232276 0.000001 MMP9 4318 -5.374221017 0.000001 MNDA 4332 -5.46504809 0.000001 MPO 4353 -6.21017394 0.000001 MSRA 4482 -3.5695742 0.000001 MYO1F 4542 -3.036870262 0.000001 MXI1 4601 -2.317837858 0.000001 MYL4 4635 -3.7057944 0.000001 MYO7B 4648 4.531387602 0.000001 NA 4663 -3.326264062 0.000001 NAGA 4668 -2.840388863 0.000001 CEACAM6 4680 -6.469511204 0.000001 NCF2 4688 -4.79784928 0.000001 272

NCF4 4689 -4.472557378 0.000001 NELL2 4753 -2.468226925 0.000001 NFIA 4774 -2.717067029 0.000001 NFE2 4778 -3.011575432 0.000001 NINJ2 4815 -2.055358883 0.000001 NKG7 4818 -3.303399318 0.000001 NKX3-1 4824 2.472968553 0.000001 NQO2 4835 -2.169345375 0.000001 NNMT 4837 -2.407695239 0.000001 NOTCH3 4854 2.454311576 0.000001 PNP 4860 -2.775152131 0.000001 NPTX2 4885 2.128468139 0.000001 NRGN 4900 -4.297739468 0.000001 NUCB2 4925 2.077645579 0.000001 NR4A2 4929 -3.037793589 0.000001 OAT 4942 -2.480676203 0.000001 OGN 4969 2.455501868 0.000001 OLR1 4973 -4.588063052 0.000001 ORM1 5004 -5.358778252 0.000001 OSM 5008 -2.213016009 0.000001 P2RX5 5026 2.416888021 0.000001 P2RY2 5029 -3.41779531 0.000001 SERPINB2 5055 -3.92089278 0.000001 PAK1 5058 -2.335954572 0.000001 PBX1 5087 -2.142956379 0.000001 PCDH9 5101 2.02804798 0.000001 PDE4B 5142 -2.804640826 0.000001 PDE4D 5144 -2.132777659 0.000001 PDK4 5166 -2.266631065 0.000001 PF4 5196 -6.378967708 0.000001 PF4V1 5197 -2.971996877 0.000001 CFP 5199 -3.506407676 0.000001 PFKFB3 5209 -2.888391969 0.000001 PFKFB4 5210 -2.740278484 0.000001 PFKP 5214 2.659655347 0.000001 CDK14 5218 -2.183797777 0.000001 PGD 5226 -2.319155384 0.000001 SERPINA1 5265 -3.538074592 0.000001 SERPINB6 5269 -3.299837955 0.000001 SERPINB8 5271 -2.004349849 0.000001 SERPINB10 5273 -4.853957459 0.000001 PIK3C2B 5287 3.253930966 0.000001 PKLR 5313 -2.61883578 0.000001 PKP2 5318 -2.524613803 0.000001 PLA2G4A 5321 -2.679519898 0.000001 273

PLAUR 5329 -2.427907581 0.000001 PLCB4 5332 2.912181961 0.000001 PLCG1 5335 3.104166434 0.000001 PLCG2 5336 -2.075728211 0.000001 PLD1 5337 -3.305119013 0.000001 PLEK 5341 -3.441790937 0.000001 PLSCR1 5359 -2.666029611 0.000001 PMAIP1 5366 2.041156264 0.000001 PPBP 5473 -6.968075421 0.000001 CTSA 5476 -2.253875578 0.000001 PPP1R3D 5509 -2.923310416 0.000001 PPP3CA 5530 -2.20068196 0.000001 PRCP 5547 -2.287639983 0.000001 SRGN 5552 -3.810962257 0.000001 PRG2 5553 -4.946521881 0.000001 PKIA 5569 2.415140514 0.000001 PRKAR2B 5577 -3.529705146 0.000001 PRKCD 5580 -3.490316806 0.000001 PRKCQ 5588 2.564182135 0.000001 PRTN3 5657 -6.784914733 0.000001 PTAFR 5724 -3.522773437 0.000001 PTGER2 5732 -2.185828037 0.000001 PTGS1 5742 -2.860929872 0.000001 PTGS2 5743 -3.808488505 0.000001 PTK2 5747 -2.728090926 0.000001 QSOX1 5768 -2.172721602 0.000001 PTPN12 5782 -2.328736491 0.000001 PTPN14 5784 2.933334043 0.000001 PTPRD 5789 3.380071685 0.000001 PTPRCAP 5790 2.108311869 0.000001 PTPRJ 5795 -3.858662731 0.000001 PTX3 5806 -4.983847603 0.000001 PXN 5829 -2.773355151 0.000001 PYGL 5836 -4.182721588 0.000001 RAB13 5872 -3.091906617 0.000001 RAB27A 5873 -3.356692285 0.000001 RFX3 5991 2.076864589 0.000001 RGS2 5997 -2.955899042 0.000001 RHAG 6005 -3.486986731 0.000001 RHD 6007 -4.551360183 0.000001 RNASE2 6036 -4.853535844 0.000001 RNASE3 6037 -6.307318391 0.000001 RNASE6 6039 -2.219636721 0.000001 RORB 6096 2.280964306 0.000001 RPS6KA2 6196 -2.608727391 0.000001 274

RPS29 6235 2.38116682 0.000001 RXRA 6256 -2.861363723 0.000001 SORT1 6272 -2.699813054 0.000001 S100A4 6275 -2.066704399 0.000001 S100A6 6277 -2.596345073 0.000001 S100A9 6280 -3.233290687 0.000001 S100A11 6282 -3.280800871 0.000001 S100A12 6283 -4.560670352 0.000001 S100P 6286 -5.403040748 0.000001 SALL2 6297 2.541023506 0.000001 SBF1 6305 2.037009483 0.000001 SCN2A 6326 2.705874093 0.000001 SCN3A 6328 3.373208578 0.000001 CCL4 6351 -2.577527566 0.000001 CCL5 6352 -3.459401934 0.000001 XCL1 6375 -2.356843931 0.000001 SDC4 6385 -2.164561648 0.000001 CXCL12 6387 -4.431348674 0.000001 SELENOP 6414 -4.853585853 0.000001 SELENOW 6415 2.273305966 0.000001 SGK1 6446 -2.310252225 0.000001 SLC2A1 6513 -2.316746277 0.000001 SLC2A3 6515 -3.08259069 0.000001 SLC2A5 6518 -4.656517808 0.000001 SLC4A1 6521 -3.404494889 0.000001 SLC5A3 6526 2.60857306 0.000001 SLC6A8 6535 -4.038813475 0.000001 SLC11A1 6556 -3.096501745 0.000001 SLC14A1 6563 -2.064775883 0.000001 SLC22A4 6583 -4.985239627 0.000001 SLIT1 6585 2.74049392 0.000001 SLPI 6590 -5.521980383 0.000001 SMARCA4 6597 2.257076919 0.000001 SNCA 6622 -3.496104234 0.000001 SNTB1 6641 -2.890552082 0.000001 SOX4 6659 4.059859929 0.000001 SPARC 6678 -2.967303738 0.000001 SPI1 6688 -2.059427407 0.000001 SPTA1 6708 -3.814909888 0.000001 SPTAN1 6709 2.266334238 0.000001 SPTB 6710 -2.417052159 0.000001 SPTBN1 6711 2.088103799 0.000001 ELOVL4 6785 4.991321157 0.000001 AURKA 6790 -2.142637439 0.000001 STX3 6809 -4.061660079 0.000001 275

TCF7 6932 2.842364707 0.000001 ZEB1 6935 2.10771094 0.000001 TCN1 6947 -6.266044412 0.000001 TMBIM6 7009 -2.20169658 0.000001 TFDP1 7027 -2.465964481 0.000001 TFDP2 7029 2.447197706 0.000001 TFF3 7033 -3.224216629 0.000001 TFR2 7036 -3.575094035 0.000001 TGFBI 7045 -4.270918258 0.000001 TGFBR1 7046 -2.192830408 0.000001 TGFBR3 7049 -2.403867956 0.000001 THBD 7056 -2.383618379 0.000001 THBS1 7057 -3.628450981 0.000001 THBS4 7060 -2.433166356 0.000001 TKT 7086 -2.610230849 0.000001 TLR1 7096 -3.863619029 0.000001 TLR2 7097 -3.569894364 0.000001 TLR4 7099 -2.617203755 0.000001 TSPAN7 7102 5.76955636 0.000001 TMOD1 7111 -3.540001726 0.000001 TNFAIP6 7130 -3.251057441 0.000001 TNFRSF1B 7133 -3.173440973 0.000001 TNS1 7145 -4.793126579 0.000001 TPM1 7168 -2.984363558 0.000001 CRISP2 7180 -3.505757526 0.000001 TRAF5 7188 3.069622981 0.000001 TRO 7216 2.01351929 0.000001 TSHR 7253 2.954107985 0.000001 TST 7263 -2.93625986 0.000001 TUBA4A 7277 -2.596410908 0.000001 TYROBP 7305 -3.738025343 0.000001 UBE2D1 7321 -2.247788146 0.000001 UGCG 7357 -3.561682134 0.000001 UPP1 7378 -2.664325767 0.000001 UROD 7389 -2.011302687 0.000001 VCAM1 7412 -3.362441524 0.000001 VCL 7414 -2.725515592 0.000001 VDR 7421 -2.563707033 0.000001 WARS 7453 -2.209892472 0.000001 WT1 7490 2.414842606 0.000001 XK 7504 -4.7074149 0.000001 ZAP70 7535 3.341505873 0.000001 ZNF711 7552 3.494332419 0.000001 ZNF91 7644 2.721465925 0.000001 ZNF185 7739 -2.220274043 0.000001 276

SLC30A4 7782 2.022370188 0.000001 ZYX 7791 -2.380816175 0.000001 LRP8 7804 2.071135668 0.000001 LAPTM5 7805 -2.059955947 0.000001 EVI5 7813 -2.009535982 0.000001 PXDN 7837 3.032362381 0.000001 IL1R2 7850 -3.300803792 0.000001 FZD5 7855 -2.670002227 0.000001 REEP5 7905 -2.659303444 0.000001 LST1 7940 -2.302722272 0.000001 TFEB 7942 -2.188856025 0.000001 TUSC3 7991 2.323451319 0.000001 TCL1A 8115 -2.040159766 0.000001 CLTCL1 8218 -3.490521839 0.000001 PNPLA4 8228 -2.137744814 0.000001 USP11 8237 2.07438153 0.000001 EPX 8288 -4.024170511 0.000001 DYSF 8291 -3.769185977 0.000001 FZD6 8323 2.233855155 0.000001 GFI1B 8328 -3.281628098 0.000001 HYAL3 8372 -2.187637194 0.000001 PIP5K1B 8395 -4.405206641 0.000001 CMAHP 8418 2.518959221 0.000001 SDPR 8436 -4.894172493 0.000001 KLF11 8462 -2.98268154 0.000001 CDC42BPA 8476 -2.309434021 0.000001 GPR65 8477 -3.114059358 0.000001 SLC43A1 8501 -2.919950309 0.000001 PIK3R3 8503 2.449620441 0.000001 DGKE 8526 2.644431869 0.000001 CST7 8530 -4.429220045 0.000001 CDC14B 8555 -3.273268998 0.000001 DEGS1 8560 -2.524991723 0.000001 PDXK 8566 -3.193302976 0.000001 PRKRA 8575 2.786274054 0.000001 PLPP3 8613 -2.207146068 0.000001 DCHS1 8642 2.704089168 0.000001 IRS2 8660 -3.528233905 0.000001 SLC4A4 8671 2.370260488 0.000001 STX11 8676 -3.995753632 0.000001 B3GALNT1 8706 -2.136648314 0.000001 TNFSF13 8741 -3.397647466 0.000001 ADAM9 8754 -2.496620054 0.000001 RIPK2 8767 -2.755399299 0.000001 SIGLEC5 8778 -3.756459448 0.000001 277

RIOK3 8780 -2.487573959 0.000001 TNFRSF10C 8794 -3.403404252 0.000001 CREG1 8804 -2.179597799 0.000001 IL18RAP 8807 -4.263187765 0.000001 LIN7A 8825 -4.055525497 0.000001 GGH 8836 -2.318912449 0.000001 HCAR3 8843 -3.246682923 0.000001 IER3 8870 -2.977614211 0.000001 VNN2 8875 -3.415192731 0.000001 VNN1 8876 -4.353683128 0.000001 CPNE3 8895 -2.787247152 0.000001 FAM127A 8933 -2.477076213 0.000001 SKAP2 8935 -3.600964571 0.000001 KYNU 8942 -2.621963626 0.000001 MGAM 8972 -4.373298195 0.000001 USP13 8975 2.062734666 0.000001 SELENBP1 8991 -5.159894965 0.000001 PGLYRP1 8993 -5.822924456 0.000001 PSTPIP2 9050 -3.0675955 0.000001 PSTPIP1 9051 -2.451111677 0.000001 MAP7 9053 -2.852469123 0.000001 SLC7A7 9056 -3.266241123 0.000001 FCGR2C 9103 -2.406448508 0.000001 ATP6V0D1 9114 -2.102933798 0.000001 SLC16A6 9120 -2.844367566 0.000001 PDLIM1 9124 -2.31558707 0.000001 SYNGR1 9145 -3.444107985 0.000001 MAP3K13 9175 -4.522999855 0.000001 REPS2 9185 -2.994087172 0.000001 DLG5 9231 2.025828315 0.000001 CCPG1 9236 -2.989892629 0.000001 CYTH2 9266 2.878193409 0.000001 ADGRG1 9289 2.359098758 0.000001 KLF4 9314 -2.926357307 0.000001 NREP 9315 4.489502024 0.000001 B4GALT6 9331 2.598552337 0.000001 CD163 9332 -3.385271669 0.000001 B4GALT5 9334 -4.350916667 0.000001 VAMP3 9341 -2.11849511 0.000001 NDST3 9348 2.775327303 0.000001 NRXN2 9379 2.983451436 0.000001 NMT2 9397 2.394414828 0.000001 CD101 9398 -2.136926288 0.000001 ARHGAP29 9411 2.123418257 0.000001 MED17 9440 2.487976929 0.000001 278

QKI 9444 -2.866839288 0.000001 LY86 9450 -2.935829355 0.000001 ITM2A 9452 2.553433829 0.000001 THEMIS2 9473 -3.767711846 0.000001 SLC4A7 9497 2.60941726 0.000001 MAGED1 9500 2.181403231 0.000001 LITAF 9516 -2.31135214 0.000001 MINPP1 9562 -3.428117606 0.000001 APOBEC3B 9582 -3.275251914 0.000001 CYTIP 9595 -2.662041073 0.000001 ARHGEF10 9639 2.817635677 0.000001 MICAL2 9645 -2.079368746 0.000001 ZNF516 9658 -2.117850637 0.000001 ST18 9705 2.82563896 0.000001 ARHGAP32 9743 2.325613834 0.000001 PHACTR2 9749 -2.270373249 0.000001 TOX 9760 4.386379807 0.000001 KIAA0513 9764 -2.899339909 0.000001 JADE3 9767 2.087578703 0.000001 RASSF2 9770 -2.59455748 0.000001 RAPGEF5 9771 2.718453361 0.000001 DLGAP5 9787 -2.164854986 0.000001 ARMCX2 9823 2.453292115 0.000001 DNAJC6 9829 -2.948954111 0.000001 ZEB2 9839 -2.170327843 0.000001 TESPA1 9840 2.1088214 0.000001 MFAP3L 9848 -3.23929822 0.000001 TMCC2 9911 -3.062912538 0.000001 KBTBD11 9920 -2.245719135 0.000001 MAFB 9935 -3.04766747 0.000001 AMMECR1 9949 -2.329817161 0.000001 SLC23A1 9963 2.130116194 0.000001 SCO2 9997 -2.202450057 0.000001 KCNE3 10008 -4.537125694 0.000001 PIGK 10026 -2.337313263 0.000001 ABCB6 10058 -2.287283508 0.000001 TRIM10 10107 -4.643327181 0.000001 ARL4A 10124 -2.137048699 0.000001 FRY 10129 -2.612590698 0.000001 NAMPT 10135 -3.059487195 0.000001 ABI2 10152 3.162569421 0.000001 PLXNC1 10154 -2.598321218 0.000001 PDZK1IP1 10158 -3.127046136 0.000001 LPAR6 10161 2.873577775 0.000001 DHRS9 10170 -3.44806429 0.000001 279

LHFPL2 10184 -2.017560392 0.000001 TRIB1 10221 -3.973864709 0.000001 CD96 10225 2.734771931 0.000001 SPRY1 10252 2.746725065 0.000001 IGSF6 10261 -3.471015087 0.000001 IRX5 10265 3.804387319 0.000001 LILRB2 10288 -4.083200996 0.000001 CRISP3 10321 -6.707307345 0.000001 SIRPB1 10326 -4.203298352 0.000001 CITED2 10370 -2.329049212 0.000001 PRG3 10394 -5.31959484 0.000001 DLC1 10395 -2.013121734 0.000001 BASP1 10409 -5.116583931 0.000001 UBAC1 10422 -2.114890819 0.000001 EIF3M 10480 2.174701718 0.000001 CRTAP 10491 -2.40580154 0.000001 CIB2 10518 2.167575833 0.000001 ATG7 10533 -2.046446428 0.000001 OLFM4 10562 -6.060113255 0.000001 NPC2 10577 -2.023180934 0.000001 GNLY 10578 -2.739080302 0.000001 ERLIN1 10613 -2.814987077 0.000001 GAS2L1 10634 -3.117896349 0.000001 IGF2BP2 10644 -2.070487283 0.000001 KLF1 10661 -4.645674147 0.000001 TNFSF13B 10673 -4.122321818 0.000001 YME1L1 10730 3.06084294 0.000001 ARPP21 10777 5.072440637 0.000001 NEK6 10783 -3.251547392 0.000001 CCR9 10803 2.479693022 0.000001 GJB6 10804 -4.511130571 0.000001 FAXDC2 10826 -3.616850869 0.000001 HPSE 10855 -2.08895369 0.000001 LILRB1 10859 -2.294764849 0.000001 ADAM28 10863 -2.180204337 0.000001 NMU 10874 -3.144128837 0.000001 FGL2 10875 -3.437564902 0.000001 SMPDL3A 10924 -2.875024554 0.000001 PRSS21 10942 -2.465510159 0.000001 MLLT11 10962 4.891100541 0.000001 CKAP4 10970 -3.712582956 0.000001 SLC27A3 11000 -2.281475892 0.000001 SLC27A2 11001 -2.957525219 0.000001 LILRA3 11026 -3.95136236 0.000001 LILRA2 11027 -2.476166476 0.000001 280

RAB31 11031 -4.975927814 0.000001 ADAP1 11033 -2.071181029 0.000001 KIF3A 11127 2.72113478 0.000001 TBC1D8 11138 -2.422079232 0.000001 NUDT5 11164 2.135404989 0.000001 IRAK3 11213 -3.830073879 0.000001 GALNT6 11226 2.464382574 0.000001 PADI2 11240 -3.55740319 0.000001 KLHL2 11275 -2.001750256 0.000001 TFEC 22797 -2.925874668 0.000001 IKZF2 22807 4.223461462 0.000001 ENPP4 22875 -2.040625186 0.000001 RUFY3 22902 3.587448565 0.000001 DHX30 22907 2.157176257 0.000001 ELL2 22936 -3.918489794 0.000001 PTGR1 22949 -2.581763027 0.000001 NINL 22981 2.807535523 0.000001 WDFY3 23001 -2.457725414 0.000001 MYT1L 23040 2.04111525 0.000001 GGA2 23062 -2.604675319 0.000001 ZNF423 23090 3.035199405 0.000001 KIF1B 23095 -2.826443972 0.000001 SPG20 23111 -2.957766641 0.000001 9/6/2017 23157 3.519170952 0.000001 GPD1L 23171 -2.447481131 0.000001 MLC1 23209 -3.113403016 0.000001 PLCL2 23228 -2.171741482 0.000001 SEL1L3 23231 -2.728761918 0.000001 MGA 23269 2.034630509 0.000001 ACSL6 23305 -3.175693398 0.000001 SYNE1 23345 -4.078584996 0.000001 ARHGEF12 23365 -3.746852524 0.000001 KIAA0368 23392 -2.276766383 0.000001 FRAT2 23401 -2.393738466 0.000001 COTL1 23406 -2.445020264 0.000001 ABCB10 23456 -2.391307332 0.000001 SPIDR 23514 -3.649071028 0.000001 PADI4 23569 -4.595172585 0.000001 CCNDBP1 23582 -2.132553194 0.000001 CLEC5A 23601 -4.813426398 0.000001 SSBP2 23635 2.860402871 0.000001 GSPT2 23708 2.312124956 0.000001 GABARAPL1 23710 -2.312718021 0.000001 OSBP2 23762 -2.451817994 0.000001 IL17RA 23765 -2.207279688 0.000001 281

QPCT 25797 -4.922190784 0.000001 BRI3 25798 -2.097209918 0.000001 GCA 25801 -4.537707347 0.000001 METTL7A 25840 -2.502550787 0.000001 DCAF12 25853 -2.337259716 0.000001 TRIM58 25893 -3.519479355 0.000001 ZNF521 25925 2.330878201 0.000001 CNRIP1 25927 -3.814006339 0.000001 SAMHD1 25939 -2.459950567 0.000001 SEC31B 25956 2.822201356 0.000001 KANK2 25959 -3.192191273 0.000001 CLIP3 25999 3.281194071 0.000001 LRP10 26020 -2.357483232 0.000001 FAM169A 26049 2.081860843 0.000001 LDLRAP1 26119 -2.298137756 0.000001 FGFR1OP2 26127 -2.084109615 0.000001 ZBTB20 26137 2.025540177 0.000001 PHGDH 26227 2.374712636 0.000001 CLEC4E 26253 -3.59251152 0.000001 FBXO6 26270 -2.547443557 0.000001 PTPN18 26469 -2.319897762 0.000001 PLEK2 26499 -2.383273634 0.000001 MYOF 26509 -2.190000709 0.000001 LATS2 26524 -2.88121021 0.000001 PCOLCE2 26577 -4.664456754 0.000001 GPR160 26996 -4.314130876 0.000001 SIGLEC7 27036 -2.291070076 0.000001 LAT 27040 2.21995871 0.000001 NSG1 27065 2.226720726 0.000001 DAPP1 27071 -2.295580054 0.000001 CIDEB 27141 -2.807331804 0.000001 COQ2 27235 -2.131905574 0.000001 SIT1 27240 2.009926904 0.000001 SLCO3A1 28232 -2.100620651 0.000001 TRBC1 28639 2.712290984 0.000001 IGLV1-44 28823 -2.546119214 0.000001 IGLJ3 28831 -2.303694389 0.000001 RGCC 28984 -2.299571691 0.000001 LGALSL 29094 -4.276841252 0.000001 CNIH4 29097 -2.033515228 0.000001 BLNK 29760 -2.552879997 0.000001 PARVB 29780 -3.306233104 0.000001 YPEL1 29799 2.450449693 0.000001 RBM15B 29890 2.203485689 0.000001 DSE 29940 -2.324184782 0.000001 282

LRP12 29967 3.465850587 0.000001 PILRA 29992 -2.532104726 0.000001 SLC40A1 30061 -2.344903964 0.000001 SOCS7 30837 2.187595782 0.000001 EHD4 30844 -2.843709028 0.000001 G0S2 50486 -3.530034972 0.000001 MYEF2 50804 3.524716491 0.000001 ASAP1 50807 -2.317530069 0.000001 HDGFRP3 50810 2.340399287 0.000001 TRAT1 50852 3.577729121 0.000001 CLEC4A 50856 -2.672143328 0.000001 STMN3 50861 2.973879604 0.000001 HEBP1 50865 -2.81625752 0.000001 ABHD5 51099 -3.544487803 0.000001 ABHD17B 51104 2.009275109 0.000001 ZNF706 51123 2.197138154 0.000001 LEF1 51176 3.759937472 0.000001 ZDHHC2 51201 -2.255880449 0.000001 MZB1 51237 3.136116251 0.000001 3/2/2017 51257 -2.245699515 0.000001 TMEM216 51259 -2.412923771 0.000001 CLEC1B 51266 -2.528446667 0.000001 KLF3 51274 -3.206695814 0.000001 SLC15A3 51296 -3.047202263 0.000001 ZDHHC3 51304 -2.089588112 0.000001 TLR8 51311 -3.660831823 0.000001 SLC25A37 51312 -3.215193655 0.000001 FAM198B 51313 -3.146854636 0.000001 NME8 51314 -3.264610506 0.000001 PLAC8 51316 -2.175030742 0.000001 MEX3C 51320 2.06650295 0.000001 AHSP 51327 -4.927323103 0.000001 MS4A4A 51338 -2.745028389 0.000001 KLRF1 51348 -2.196204335 0.000001 CHST15 51363 -2.479714683 0.000001 AIG1 51390 -2.667974288 0.000001 SNX9 51429 -2.539957412 0.000001 EVL 51466 2.512253059 0.000001 PEX5L 51555 3.935450502 0.000001 SLC25A39 51629 -2.471651573 0.000001 CYB5R1 51706 -2.010973694 0.000001 MSRB1 51734 -4.0011338 0.000001 TM6SF1 53346 -3.596453542 0.000001 UBASH3A 53347 2.32800305 0.000001 P2RY13 53829 -4.626140074 0.000001 283

GPR84 53831 -4.344760822 0.000001 GSAP 54103 -3.448397064 0.000001 TREM1 54210 -3.689969368 0.000001 CYTL1 54360 -2.521627694 0.000001 RIN2 54453 -2.710094123 0.000001 PLEKHA5 54477 2.851162059 0.000001 FAM105A 54491 -2.360585854 0.000001 RBM47 54502 -3.433139379 0.000001 CPVL 54504 -2.461645217 0.000001 EGLN1 54583 -2.453716697 0.000001 LZTFL1 54585 3.03031243 0.000001 MANSC1 54682 -3.615713387 0.000001 LEPROT 54741 -2.05433433 0.000001 TET2 54790 -2.755055311 0.000001 AHI1 54806 2.799237644 0.000001 DEF8 54849 -2.049216952 0.000001 FAM46C 54855 -3.392241595 0.000001 PXK 54899 -2.007195777 0.000001 LPCAT2 54947 -2.852083779 0.000001 C20orf27 54976 -2.05272164 0.000001 ZSCAN2 54993 2.199187438 0.000001 TESC 54997 -2.594596871 0.000001 VPS37C 55048 -2.635142803 0.000001 FLJ10038 55056 2.108928152 0.000001 WIPI1 55062 -3.918665672 0.000001 ANO10 55129 -2.669184258 0.000001 SLC25A36 55186 2.031983328 0.000001 NUDT11 55190 2.105974339 0.000001 DPPA4 55211 -2.069904797 0.000001 LRRC1 55227 2.483246611 0.000001 STEAP3 55240 -3.844598601 0.000001 ELP2 55250 2.261321084 0.000001 ZNF444 55311 2.207747594 0.000001 CPPED1 55313 -2.343296846 0.000001 DRAM1 55332 -2.698612618 0.000001 VNN3 55350 -3.558329133 0.000001 LAPTM4B 55353 -2.753453439 0.000001 SLC22A15 55356 -4.511153965 0.000001 HEMGN 55363 -3.527514807 0.000001 IMPACT 55364 -3.279364347 0.000001 STRADB 55437 -2.681349911 0.000001 DNAJA4 55466 -2.626251847 0.000001 TMEM55A 55529 -3.26831062 0.000001 SOX6 55553 -3.36956127 0.000001 FAM46A 55603 -3.797648121 0.000001 284

TASP1 55617 2.947320455 0.000001 CHD7 55636 -2.985604433 0.000001 RAB20 55647 -2.452030502 0.000001 ARHGEF40 55701 -2.268427797 0.000001 FAR2 55711 -3.124271708 0.000001 N4BP2 55728 2.422591786 0.000001 TMEM30A 55754 -2.122847569 0.000001 UGGT2 55757 -2.291350103 0.000001 C1orf106 55765 -2.057805723 0.000001 CSGALNACT 1 55790 -2.501007984 0.000001 CACNA2D3 55799 -2.256249542 0.000001 BEX1 55859 -4.35745759 0.000001 APOBR 55911 -2.591921732 0.000001 SULF2 55959 -2.961478311 0.000001 AJAP1 55966 2.260443548 0.000001 PDGFC 56034 -3.510688959 0.000001 MOSPD1 56180 -2.133753384 0.000001 GPCPD1 56261 -2.217193279 0.000001 PARD3 56288 3.705927708 0.000001 SUCNR1 56670 -2.77790716 0.000001 TMEM9B 56674 -2.221272072 0.000001 RETN 56729 -4.264704612 0.000001 MAP3K7CL 56911 -2.092792262 0.000001 LXN 56925 -2.102109434 0.000001 PRTFDC1 56952 3.679554403 0.000001 CHPT1 56994 -2.130246992 0.000001 AGTRAP 57085 -3.791062939 0.000001 NAT14 57106 2.699409319 0.000001 CD177 57126 -4.138631788 0.000001 APMAP 57136 -2.56914535 0.000001 RTN4 57142 2.067111525 0.000001 TMCC3 57458 -2.540570482 0.000001 ZNF512B 57473 2.486177577 0.000001 RNF150 57484 2.45582973 0.000001 MKL2 57496 2.199344299 0.000001 NLGN4X 57502 2.494955268 0.000001 NCEH1 57552 -2.959146476 0.000001 PCDH10 57575 2.396733456 0.000001 PREX1 57580 -2.075049152 0.000001 BAHCC1 57597 3.27230467 0.000001 PITPNM2 57605 2.179556951 0.000001 SHTN1 57698 -2.283374335 0.000001 DENND1A 57706 -2.125945819 0.000001 ZNF529 57711 2.365548722 0.000001 285

ANKRD36B 57730 2.229467758 0.000001 HES4 57801 3.13982278 0.000001 SLAMF7 57823 -2.618181963 0.000001 CXCL16 58191 -2.874755606 0.000001 SQRDL 58472 -2.010014522 0.000001 MS4A7 58475 -2.832268411 0.000001 NLRC4 58484 -3.235806304 0.000001 HIVEP3 59269 2.461388858 0.000001 SCPEP1 59342 -3.553758266 0.000001 PROK2 60675 -4.28360334 0.000001 GALNT11 63917 -2.654337617 0.000001 KIF13A 63971 -3.252878621 0.000001 SLC28A3 64078 -3.593242485 0.000001 LRRC4 64101 -2.907030385 0.000001 C10orf54 64115 -2.418696367 0.000001 NOD2 64127 -3.124623523 0.000001 SEMA4A 64218 -2.847977141 0.000001 MS4A6A 64231 -3.473523049 0.000001 MMP25 64386 -2.99808395 0.000001 ZMAT3 64393 2.583444985 0.000001 HHIP 64399 5.011947574 0.000001 AKTIP 64400 -2.748463674 0.000001 RGS18 64407 -3.771383788 0.000001 ARAP3 64411 -2.213324577 0.000001 CLEC7A 64581 -3.344895707 0.000001 MFSD1 64747 -2.249456222 0.000001 CCDC136 64753 2.852114342 0.000001 3/1/2017 64757 -4.163693635 0.000001 FNDC3B 64778 -3.415044635 0.000001 RFX7 64864 2.100135001 0.000001 BCL11B 64919 2.995951743 0.000001 TMEM237 65062 2.53006876 0.000001 MARCKSL1 65108 2.528979953 0.000001 NADK 65220 -2.295815407 0.000001 C11orf95 65998 2.870223455 0.000001 TRAK2 66008 -2.699381053 0.000001 LINC01260 79015 3.30228907 0.000001 PRRG4 79056 -4.068770891 0.000001 LILRA6 79168 -2.221300435 0.000001 EFHD2 79180 -2.097683049 0.000001 IRX3 79191 2.598825333 0.000001 OGFRL1 79627 -2.483392218 0.000001 ROGDI 79641 -2.879619682 0.000001 STEAP4 79689 -2.720053399 0.000001 MICALL2 79778 -2.410758218 0.000001 286

PLBD1 79887 -5.571732251 0.000001 ATP8B4 79895 -4.716584292 0.000001 CYBRD1 79901 -3.430050677 0.000001 SCD5 79966 3.605320552 0.000001 ATAT1 79969 2.020761578 0.000001 WLS 79971 -2.418810719 0.000001 PCNX2 80003 2.563889337 0.000001 PGGHG 80162 2.890772834 0.000001 RUNX1-IT1 80215 2.014426849 0.000001 FAM214B 80256 -2.116925328 0.000001 PLEKHO2 80301 -2.27957873 0.000001 CPEB4 80315 -3.023122195 0.000001 GKAP1 80318 2.121591856 0.000001 CEP70 80321 3.566103925 0.000001 NPL 80896 -4.248789104 0.000001 TUBB1 81027 -5.822467603 0.000001 ZBP1 81030 -2.190465893 0.000001 FAM49A 81553 -2.251367923 0.000001 CSRNP2 81566 2.334188632 0.000001 VANGL1 81839 2.090320151 0.000001 ARHGAP24 83478 -3.239552521 0.000001 FAM107B 83641 -2.836103693 0.000001 CRISPLD2 83716 -3.671961972 0.000001 ROPN1L 83853 -2.960674792 0.000001 TMEM120A 83862 -2.228099657 0.000001 CDCA7 83879 3.057564805 0.000001 FGFBP2 83888 -2.255915034 0.000001 RASSF4 83937 -2.751540115 0.000001 C10orf11 83938 -3.316788552 0.000001 B3GNT5 84002 -2.091125244 0.000001 PRAM1 84106 -3.378937594 0.000001 ANTXR1 84168 2.232502656 0.000001 MEX3B 84206 2.966179951 0.000001 LDOC1L 84247 3.722309245 0.000001 C2orf88 84281 -3.074064361 0.000001 ZBED3 84327 2.437848305 0.000001 CYSTM1 84418 -3.499630604 0.000001 TUBB6 84617 -3.263103447 0.000001 CARD6 84674 -2.073245649 0.000001 LOXL3 84695 -2.308090341 0.000001 CBX2 84733 2.332704674 0.000001 GPAT3 84803 -4.394802393 0.000001 NFKBID 84807 -2.279772677 0.000001 ZNF496 84838 2.716839812 0.000001 SLC7A3 84889 2.314045967 0.000001 287

PLXDC2 84898 -2.241855385 0.000001 ZNRF1 84937 3.505922559 0.000001 LGALS12 85329 -2.743378903 0.000001 SLC22A16 85413 -2.250821891 0.000001 ITPRIP 85450 -2.134079582 0.000001 TANC1 85461 2.76317007 0.000001 FHDC1 85462 -2.800101859 0.000001 UMODL1 89766 3.288772421 0.000001 KCNK17 89822 2.952778056 0.000001 SLC25A21 89874 -3.06842757 0.000001 CEACAM21 90273 -3.038052723 0.000001 C5orf30 90355 -2.264022441 0.000001 TMEM263 90488 2.456721658 0.000001 CCDC126 90693 -2.08017938 0.000001 CDKN2AIPN L 91368 2.188887184 0.000001 NLRP12 91662 -2.600786498 0.000001 MYADM 91663 -2.227635998 0.000001 TIMD4 91937 -2.016645226 0.000001 ZC3HAV1L 92092 2.059798443 0.000001 SCGB3A1 92304 2.973863649 0.000001 PXYLP1 92370 2.428767475 0.000001 SPECC1 92521 -2.123418172 0.000001 DTD1 92675 -2.37866026 0.000001 TBC1D31 93594 2.168515667 0.000001 BMS1P20 96610 -2.629825028 0.000001 SNX18 112574 -2.653574172 0.000001 GLMP 112770 -2.102446218 0.000001 LACTB 114294 -2.278278184 0.000001 NLRP3 114548 -2.114109861 0.000001 ERMAP 114625 -2.489790804 0.000001 OSBPL1A 114876 -3.504927759 0.000001 MB21D1 115004 -2.18390264 0.000001 3/3/2017 115123 -2.528856805 0.000001 KCTD12 115207 -2.056883952 0.000001 FCRL1 115350 -2.290709951 0.000001 FCHO2 115548 -3.938079373 0.000001 CTHRC1 115908 4.929597716 0.000001 FAM210B 116151 -3.258824369 0.000001 RBP7 116362 -4.494857195 0.000001 SLC26A8 116369 -2.167510436 0.000001 RAB39B 116442 2.01090549 0.000001 LRG1 116844 -3.074046658 0.000001 TAGAP 117289 -2.18248663 0.000001 PIK3AP1 118788 -2.289563549 0.000001 288

ANKRD22 118932 -2.930145188 0.000001 PDZD8 118987 -2.214535446 0.000001 SLC36A4 120103 -2.017046423 0.000001 JAML 120425 -2.631437288 0.000001 LRRK2 120892 -3.607190496 0.000001 TMEM52B 120939 -2.451116538 0.000001 FGD4 121512 -2.719127177 0.000001 ANKRD9 122416 -2.097327908 0.000001 JDP2 122953 -3.273942013 0.000001 TTC8 123016 -2.181643865 0.000001 SLC24A4 123041 -2.386412755 0.000001 ZG16B 124220 -2.492805848 0.000001 MSI2 124540 3.346897413 0.000001 OSCAR 126014 -3.27256084 0.000001 ZNF57 126295 3.264927822 0.000001 LRRC25 126364 -2.341841246 0.000001 ANKLE1 126549 -2.631268222 0.000001 TPRG1L 127262 -2.081294201 0.000001 C1orf162 128346 -4.03117667 0.000001 TANGO2 128989 -2.033384733 0.000001 MBOAT2 129642 -3.451087945 0.000001 OCIAD2 132299 2.498202082 0.000001 PABPC4L 132430 2.730487841 0.000001 C4orf32 132720 -2.448406057 0.000001 CPEB2 132864 -2.11047014 0.000001 CD109 135228 2.632143348 0.000001 TMEM71 137835 -2.225218159 0.000001 NACC2 138151 -2.848596424 0.000001 MYL6B 140465 2.470452594 0.000001 SRXN1 140809 -2.819639222 0.000001 FAM65C 140876 -4.0321336 0.000001 SIRPA 140885 -3.291738874 0.000001 SESN3 143686 -3.313285102 0.000001 PIWIL4 143689 -2.610761645 0.000001 GLT1D1 144423 -3.296094809 0.000001 CEP128 145508 2.536712201 0.000001 TTC7B 145567 -2.291896561 0.000001 CARMIL2 146206 2.314406539 0.000001 CD300LF 146722 -3.490842691 0.000001 SLFN13 146857 -2.416561378 0.000001 ZNF738 148203 2.720997675 0.000001 TMEM56 148534 -4.771525847 0.000001 ABCC13 150000 -2.346414723 0.000001 NFAM1 150372 -3.106627152 0.000001 FBXO41 150726 2.478795099 0.000001 289

LINC00342 150759 2.323321589 0.000001 SLC16A14 151473 -2.164070375 0.000001 CCDC50 152137 -2.906674163 0.000001 ZNF827 152485 3.887203185 0.000001 PLEKHG4B 153478 2.02497409 0.000001 RNF217 154214 -2.62480557 0.000001 ABCA13 154664 -3.877550399 0.000001 RASEF 158158 2.279174226 0.000001 FAM201A 158228 -2.109244039 0.000001 CLEC12A 160364 -4.821408212 0.000001 ZFP1 162239 2.311463578 0.000001 ZNF709 163051 2.595308421 0.000001 SGMS2 166929 -3.1446231 0.000001 ZNF467 168544 -2.442052542 0.000001 TMEM64 169200 -2.125506072 0.000001 C10orf128 170371 -2.103617981 0.000001 ADAMTS17 170691 2.542067762 0.000001 AAED1 195827 -2.871142182 0.000001 DTX3 196403 2.627804577 0.000001 PLBD2 196463 -2.236378759 0.000001 MCEMP1 199675 -4.66240675 0.000001 FLCN 201163 -2.075829978 0.000001 SLC39A11 201266 -2.249311175 0.000001 CCDC125 202243 -2.148392446 0.000001 GAPT 202309 -2.710448315 0.000001 SUSD3 203328 -2.528831014 0.000001 SLC44A5 204962 2.47167877 0.000001 SLC36A1 206358 -2.701850072 0.000001 YPEL4 219539 -2.712832747 0.000001 MPEG1 219972 -4.300759011 0.000001 RASGEF1A 221002 -2.342125304 0.000001 CPNE2 221184 -2.825094789 0.000001 FGD2 221472 -2.68033208 0.000001 RNF182 221687 -2.901286952 0.000001 PHACTR1 221692 -2.080992336 0.000001 JAZF1 221895 -2.970592319 0.000001 ADGRG3 222487 -3.439320362 0.000001 TSPO2 222642 -3.948090699 0.000001 ZBTB38 253461 -2.517787861 0.000001 MSRB3 253827 -2.696722908 0.000001 RNF144B 255488 -2.886752337 0.000001 NATD1 256302 -2.716630369 0.000001 FRMD3 257019 -2.670808792 0.000001 SGMS1 259230 -2.554077372 0.000001 RGL4 266747 -3.153747919 0.000001 290

RASSF3 283349 -2.314880915 0.000001 GAS2L3 283431 -3.946117329 0.000001 C17orf58 284018 -2.535581239 0.000001 METRNL 284207 -2.289040175 0.000001 KCTD1 284252 4.166392298 0.000001 VSTM1 284415 -4.508621083 0.000001 SIRPB2 284759 -2.057867497 0.000001 SEC14L4 284904 -3.641467118 0.000001 LOC285097 285097 2.552478226 0.000001 LINC01268 285758 -2.261983312 0.000001 SCAI 286205 3.957479549 0.000001 TUSC1 286319 -3.013001149 0.000001 EIF4E3 317649 -2.452460661 0.000001 RFESD 317671 -3.146324147 0.000001 CLEC4D 338339 -4.115840208 0.000001 SPOPL 339745 -2.480264121 0.000001 KIAA1211L 343990 -2.510671986 0.000001 PAQR9 344838 -3.098808471 0.000001 SLCO4C1 353189 -3.072016775 0.000001 LILRA5 353514 -2.712184581 0.000001 B3GNT8 374907 -2.000379996 0.000001 MAST4 375449 2.352123682 0.000001 RAB15 376267 2.520594219 0.000001 CEP85L 387119 2.423664221 0.000001 ILDR2 387597 2.065892592 0.000001 SHISA2 387914 4.151406169 0.000001 LINC01550 388011 2.812427506 0.000001 SBK1 388228 3.201693377 0.000001 SMIM1 388588 -2.801967413 0.000001 LINC01120 389043 2.704050813 0.000001 ARHGEF37 389337 -2.405021951 0.000001 NUDT19 390916 -2.197352774 0.000001 CASC15 401237 4.331409469 0.000001 HACD4 401494 -2.865305756 0.000001 BCL2L15 440603 -3.085790493 0.000001 C5orf56 441108 2.179100571 0.000001 DUXAP10 503639 2.225427955 0.000001 ZNF704 619279 2.388120537 0.000001 LEF1-AS1 641518 2.813397689 0.000001 FAM110C 642273 3.169374364 0.000001 SMIM5 643008 -2.455116211 0.000001 LOC643072 643072 -2.132897736 0.000001 ECRP 643332 -4.93154213 0.000001 CRNDE 643911 4.33364158 0.000001 HHIP-AS1 646576 5.583754583 0.000001 291

ZBTB8A 653121 4.012866573 0.000001 GXYLT2 727936 3.62175731 0.000001 PCAT18 728606 4.164636518 0.000001 CCR2 729230 -2.439308225 0.000001 BORCS8 729991 -2.450585473 0.000001 H2BFXP 767811 4.419125832 0.000001 GABPB1-AS1 1E+08 2.580885669 0.000001 DPY19L1P1 1E+08 -2.647789956 0.000001 LOC10013047 6 1E+08 2.621417425 0.000001 CD24 1E+08 -4.781722589 0.000001 C17orf99 1E+08 -3.59488839 0.000001 HBD 1E+08 -3.825017711 0.000001 BRE-AS1 1E+08 -2.014027717 0.000001 LOC10050550 1.01E+0 1 8 4.590390302 0.000001 1.01E+0 TRG-AS1 8 2.674648621 0.000001 LOC10106039 1.01E+0 1 8 3.468018873 0.000001 LOC10192800 1.02E+0 0 8 2.171255519 0.000001 LOC10192804 1.02E+0 7 8 -2.240121692 0.000001 LOC10192842 1.02E+0 9 8 -3.9014807 0.000001 1.02E+0 LINC01215 8 3.026615898 0.000001 LOC10260646 1.03E+0 5 8 2.464099788 0.000001 1.05E+0 LUNAR1 8 2.021138267 0.000001

292

Appendix P

Comprehensive KEGG pathway analysis

with adjusted p-values

293

Appendix P KEGG biologic pathways with adjusted p-value

KEGG Biologic Pathway p-value

Hematopoietic cell lineage 2.48E-09

Porphyrin and chlorophyll metabolism 9.21E-06

Amoebiasis 8.49E-05

Leishmaniasis 0.000144

Asthma 0.000159

Transcriptional misregulation in cancer 0.000238

Cell adhesion molecules (CAMs) 0.000317

Nitrogen metabolism 0.000731

Cytokine-cytokine receptor interaction 0.00134

Th17 cell differentiation 0.002142

Malaria 0.002236

Chemokine signaling pathway 0.003569

Staphylococcus aureus infection 0.004485

Phagosome 0.00467

B cell receptor signaling pathway 0.008874

Starch and sucrose metabolism 0.009194

Rheumatoid arthritis 0.009949

Metabolic pathways 0.011156

NOD-like receptor signaling pathway 0.013287

IL-17 signaling pathway 0.01339

Leukocyte transendothelial migration 0.016098

Tuberculosis 0.016413

Osteoclast differentiation 0.0184

294

Proximal tubule bicarbonate reclamation 0.023043

NF-kappa B signaling pathway 0.023488

Chemical carcinogenesis 0.025585

ABC transporters 0.028042

Signaling pathways regulating pluripotency of stem cells 0.029842

Renin secretion 0.030587

Glycolysis / Gluconeogenesis 0.032415

Thyroid cancer 0.036899

Inflammatory mediator regulation of TRP channels 0.037925

Jak-STAT signaling pathway 0.039831

Retrograde endocannabinoid signaling 0.040873

Insulin signaling pathway 0.054338

Pancreatic secretion 0.054634

Th1 and Th2 cell differentiation 0.055837

MicroRNAs in cancer 0.056372

Epstein-Barr virus infection 0.061012

Regulation of lipolysis in adipocytes 0.061292

Renin-angiotensin system 0.061826

Arachidonic acid metabolism 0.063796

Proteoglycans in cancer 0.065633

Metabolism of xenobiotics by cytochrome P450 0.067218

Hypertrophic cardiomyopathy (HCM) 0.067437

Primary immunodeficiency 0.072061

Arrhythmogenic right ventricular cardiomyopathy (ARVC) 0.072776

Inflammatory bowel disease (IBD) 0.074246

295

Prolactin signaling pathway 0.075076

ECM-receptor interaction 0.075134

Bladder cancer 0.078161

Glycine, serine and threonine metabolism 0.081915

Mineral absorption 0.08231

Salivary secretion 0.082391

HTLV-I infection 0.084999

Salmonella infection 0.085941

Tight junction 0.089749

Oocyte meiosis 0.091742

Endocrine resistance 0.09191

Hepatitis B 0.094906

Dilated cardiomyopathy 0.097864

Neuroactive ligand-receptor interaction 0.098951

Rap1 signaling pathway 0.099749

TNF signaling pathway 0.100564

PI3K-Akt signaling pathway 0.101339

Riboflavin metabolism 0.103445

Long-term potentiation 0.104565

Steroid hormone biosynthesis 0.108865

Pathways in cancer 0.10944

Adipocytokine signaling pathway 0.112187

Fc epsilon RI signaling pathway 0.1129

Ovarian steroidogenesis 0.112933

Insulin resistance 0.114762

296

Adherens junction 0.115252

Phospholipase D signaling pathway 0.117304

Mannose type O-glycan biosynthesis 0.117462

Systemic lupus erythematosus 0.117492

Complement and coagulation cascades 0.12159

Intestinal immune network for IgA production 0.124084

Amino sugar and nucleotide sugar metabolism 0.126463

Bile secretion 0.127108

Neomycin, kanamycin and gentamicin biosynthesis 0.127592

Influenza A 0.129975

Adrenergic signaling in cardiomyocytes 0.131371

Viral myocarditis 0.134862

Histidine metabolism 0.135603

African trypanosomiasis 0.136745

Glutathione metabolism 0.144997

Viral carcinogenesis 0.146301

Legionellosis 0.147813

Prostate cancer 0.160775

PPAR signaling pathway 0.161731

Nicotinate and nicotinamide metabolism 0.16384

Glycosphingolipid biosynthesis - lacto and neolacto series 0.16384

Collecting duct acid secretion 0.16384

Hepatitis C 0.166686

Pathogenic Escherichia coli infection 0.167342

Drug metabolism - cytochrome P450 0.170968

297

Graft-versus-host disease 0.173134

Dorso-ventral axis formation 0.180828

Antifolate resistance 0.181371

TGF-beta signaling pathway 0.185623

Type I diabetes mellitus 0.190534 p53 signaling pathway 0.191325

Galactose metabolism 0.192987

GnRH signaling pathway 0.193639 mTOR signaling pathway 0.202533

Allograft rejection 0.204693

Small cell lung cancer 0.211927

Glycerolipid metabolism 0.212091

Drug metabolism - other enzymes 0.212765

Type II diabetes mellitus 0.214656

EGFR tyrosine kinase inhibitor resistance 0.2152

Glycerophospholipid metabolism 0.21852

SNARE interactions in vesicular transport 0.219589

Wnt signaling pathway 0.227901

Melanoma 0.233678

Autoimmune thyroid disease 0.233957

Toxoplasmosis 0.235522

Dopaminergic synapse 0.236357

Apoptosis 0.237731

Focal adhesion 0.238129

Measles 0.24554

298

Endometrial cancer 0.250452

Estrogen signaling pathway 0.250864

Acute myeloid leukemia 0.254518

Huntington's disease 0.25886

Carbohydrate digestion and absorption 0.272196

Amyotrophic lateral sclerosis (ALS) 0.273546

Non-homologous end-joining 0.279386

Pyruvate metabolism 0.282932

Bacterial invasion of epithelial cells 0.283422

Biosynthesis of amino acids 0.284335

Fc gamma R-mediated phagocytosis 0.284569

HIF-1 signaling pathway 0.293952

Fatty acid biosynthesis 0.298801

Gastric acid secretion 0.299381

FoxO signaling pathway 0.300544

Sphingolipid metabolism 0.302978

ErbB signaling pathway 0.311277

RIG-I-like receptor signaling pathway 0.319536

Thyroid hormone signaling pathway 0.320243

Cardiac muscle contraction 0.321271

Pertussis 0.324869

Glucagon signaling pathway 0.335331

Taste transduction 0.337563

Cytosolic DNA-sensing pathway 0.348707

Carbon metabolism 0.350224

299

Retinol metabolism 0.352547

Phenylalanine metabolism 0.353972

Maturity onset diabetes of the young 0.356614

Antigen processing and presentation 0.360476

Progesterone-mediated oocyte maturation 0.366264

Peroxisome 0.37296

Platelet activation 0.374266

Shigellosis 0.376206

Non-small cell lung cancer 0.376566

Alzheimer's disease 0.37769

Serotonergic synapse 0.379936

Hippo signaling pathway -multiple species 0.383688

Pantothenate and CoA biosynthesis 0.388323

Platinum drug resistance 0.389786

Lysosome 0.393087

Protein digestion and absorption 0.394913

Gap junction 0.396606

Hedgehog signaling pathway 0.401247

Steroid biosynthesis 0.404808

Neurotrophin signaling pathway 0.405239

Long-term depression 0.41059

Cholinergic synapse 0.415363

Oxytocin signaling pathway 0.416749

Arginine biosynthesis 0.42085

Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate 0.42085

300

Amphetamine addiction 0.424216

Parkinson's disease 0.431895

Alcoholism 0.436356

Notch signaling pathway 0.445825

Choline metabolism in cancer 0.448527

Chronic myeloid leukemia 0.453911

Aldosterone synthesis and secretion 0.458297

Natural killer cell mediated cytotoxicity 0.459138

Vasopressin-regulated water reabsorption 0.470429

Regulation of actin cytoskeleton 0.473862

Phosphatidylinositol signaling system 0.480158

Vascular smooth muscle contraction 0.484929

Hippo signaling pathway 0.492976

Synaptic vesicle cycle 0.500413

AMPK signaling pathway 0.514837

Central carbon metabolism in cancer 0.529736

Glyoxylate and dicarboxylate metabolism 0.534584

Axon guidance 0.538053

Calcium signaling pathway 0.544825

Cell cycle 0.546358

Mucin type O-glycan biosynthesis 0.547134

GABAergic synapse 0.550017

Circadian entrainment 0.556376

Inositol phosphate metabolism 0.558007

Apoptosis - multiple species 0.559346

301

beta-Alanine metabolism 0.571229

Fructose and mannose metabolism 0.582792

MAPK signaling pathway 0.597345

AGE-RAGE signaling pathway in diabetic complications 0.601934

VEGF signaling pathway 0.608713

Colorectal cancer 0.612034

Tyrosine metabolism 0.615648

Endocrine and other factor-regulated calcium reabsorption 0.615842

Longevity regulating pathway - multiple species 0.621728

Sphingolipid signaling pathway 0.631786

Insulin secretion 0.637499

Purine metabolism 0.642954 cAMP signaling pathway 0.651915

Fat digestion and absorption 0.655473

Endocytosis 0.660919

Tryptophan metabolism 0.664768

Ether lipid metabolism 0.664768

Herpes simplex infection 0.670142

Toll-like receptor signaling pathway 0.673318

Breast cancer 0.678469

Fatty acid degradation 0.682614

Melanogenesis 0.686893

Glioma 0.692895 cGMP-PKG signaling pathway 0.69746

Cysteine and methionine metabolism 0.699511

302

Arginine and proline metabolism 0.70762

Fatty acid metabolism 0.70762

Olfactory transduction 0.725641

Pyrimidine metabolism 0.729342

Non-alcoholic fatty liver disease (NAFLD) 0.771944

T cell receptor signaling pathway 0.775075

Chagas disease (American trypanosomiasis) 0.799349

Cocaine addiction 0.833497

Epithelial cell signaling in Helicobacter pylori infection 0.834297

Thyroid hormone synthesis 0.89347

Longevity regulating pathway 0.929651

Pancreatic cancer 0.939527

Basal cell carcinoma 0.950819

Glutamatergic synapse 0.961856

Ras signaling pathway 0.997715

Protein processing in endoplasmic reticulum 0.999302

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Appendix Q

Curriculum Vitae of Dr Robert P Weinberg, DO, JD

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CURRICULUM VITAE

Name: Robert Weinberg, DO, JD Citizenship: U.S. Address: 23 Lapham Street Marital status: married (w/ 3 sons) Medford, MA 02155 Email: [email protected] Phone (617) 460-7898 Fax (617) 977-0902

Position Seeking (2017): Mid-management position in bio-pharma working as Medical Director for drug development & global clinical trials; Drug surveillance & safety monitoring; post-marketing surveillance

Recent Biomedical Research Experience

2016-17 Graduate Thesis research (MMSc, expected November 2017) Harvard Medical School, bioinformatics and computational genomic analyses investigating the role of the zinc- finger transcription factor SALL4 on leukemogenesis during the development of acute lymphoblastic leukemia

2013-17 Board Member & Co-Chair, Hummingbird IRB (www.hummingbirdirb.com) Reviews research & clinical trials involving human subjects per regulations (NIH, DHHS,FDA)

2010-17 Research Scientist, M.I.T., Biomaterials Science & Engineering Laboratory Bioinformatics and computational genomic analyses investigating the transcriptomic changes induced in mice by oil palm phenolic compounds and investigating potential new Alzheimer’s drugs from phytochemical extracts Working under Professor ChoKyun Rha

2008-09 Biotechnology Specialist, Cardinal Intellectual Property, Patent Analyst, Evanston, Il Reviews PCT applications of medical devices, biotechnology patents Database searches on DNA sequences, recombinant DNA products

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Education, Degrees and Certificates

2014-17 MMSc in Immunology candidate, Harvard Medical School, Boston, MA Expecting MMSc. in Immunology degree in June 2017

2004-06 New England School of Law, Boston, MA Awarded J.D. cum laude in May 2006, concentration in Intellectual Property and Patent Law

1996 Certification as Fellow of American Board of Certification in Family Medicine, American Association of Physician Specialists, Atlanta, GA

1994-95 Family Practice Resident, Lawrence General Hospital, Lawrence,MA

1987-88 Internal Medicine Resident, St. Vincent Hospital, Worcester, MA University of Massachusetts Medical school hospital

1986-87 Anatomic Pathology Resident, Roger Williams General Hospital Brown University Integrated Pathology program, Providence, RI

1982-86 New York College of Osteopathic Medicine, Old Westbury, NY Awarded Doctor of Osteopathic Medicine degree in June 1986

1971-76 Massachusetts Institute of Technology (M.I.T.), Cambridge, MA Awarded B.S. in Biology degree in June 1976

Academic Appointments

2012-2014 Instructor, Dept of Medicine, Boston University School of Medicine, Boston Teaching first- and second-year medical students Integrated Pathology (IP) and Introduction to Physical Diagnosis (ICM) [part-time per diem]

2009-2014 Adjunct Faculty, Dept of Physics, Eastern Nazarene College, Quincy, MA Taught college-level physics, chemistry, astronomy, algebra [part-time per diem]

2009-2010 Adjunct Faculty, Dept of Health Sciences, Bay State College, Boston, MA Taught Anatomy & Physiology, Biology, Medical Office Procedures 306

2006-2008 Adjunct Faculty, Dept of Mathematics, Newbury College, Brookline, MA Taught college algebra, consumer mathematics, pre-algebra [part-time per diem]

1990-98 Instructor, Department of Community Health & Family Practice, Tufts University School of Medicine, Boston, MA Taught Intro to Clinical Skills, Differential Diagnosis & Problem-based Learning [part-time per diem]

1990-92 Advanced Cardiac Life Support Instructor, Beth Israel Hospital, Boston Certified by American Heart Association to teach ER docs & physicians Protocols for resuscitation of persons succumbing to cardiac arrest

1991-92 Advanced Trauma Life Support Instructor-candidate, Longwood Area Trauma Certified by American College of Surgeons to teach trauma skills

Professional Association Committee Activities

2015-17 Disaster Response and Preparedness Committee member American College of Emergency Medicine (ACEP)

Additional Biomedical Research Experience

1981-82 Research Associate, Center for Blood Research, Boston, MA

Production of monoclonal antibodies to HLA antigens

Fusion & tissue culture of mouse hybridoma cells

In vivo propagation of hybridoma cells in mouse ascites

Immunofluorescent and tagged antibody identification

1979-80 Research Specialist, University of Pennsylvania Medical School

Study of platelet membrane glycoproteins in autoimmune thrombocytopenic purpuras

Polyacrylamide gel electrophoresis of membrane glycoproteins

Gel filtration column chromatography, ion exchange chromatography

I-125 radio-labelling of cellular membrane proteins & gamma counter

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1977-78 Immunology Research Technician, Children’s Hospital, Boston

Production & immunochemical characterization of idiotypic binding sites on human

immunoglobulins

Column chromatography: gel filtration, ion exchange, affinity

Ouchterlony gel immunoprecipitation

Preparation in rabbits of anti-human immunoglobulin antisera

T-cell and B-cell assays, rosetting assays, receptor assays

Analysis of gamma and mu cell surface receptors

1974-76 Cancer Research Institute, New England Deaconess Hospital

Virological & immunogenetic studies on NEDH Kx rat strain

Tissue homogenization and in vitro enzyme bio-assays

Rat Embryo Tissue Culture (RETC), viral propagation through tissue culture

Viral agglutination assays, viral titers by hemagglutination

1971-72 Research Assistant, Animal Pathology Laboratory, M.I.T., Cambridge, MA

studies on the histopathology of lipotrope-induced changes in the GI tract of rats work done under Dr. Paul Newberne and Adrienne Rogers

1970-71 Research program, Waldemar Medical Foundation, Long Island, NY microbial screening for bacteria capable of bio-degrading various detergents

Publications & Biographic Essays in Alumni Journals

Weinberg, RP (2017) “Identification of cell surface markers which correlate with SALL4 in a B-cell acute lymphoblastic leukemia with translocaiton t(8;14) discovered through bioinformatic analysis of microarray gene expression data” MMSc thesis, to be submitted to Harvard University Archive, June 2017

Weinberg RP, Koledova VV, Shin H et al. (2017). “Oil palm phenolics inhibit the in vitro aggregation of beta-amyloid peptide into oligomeric complexes” (manuscript submitted)

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“ Anti-Idiotypic Antisera in Man. I. Production and Immunochemical characterization of anti-idio-typic antisera to human antitetanus antibodies,” Raif S. Geha and Robert P. Weinberg, Journal of Immunology, Vol.121, No.4, October 1978

“Sky King,” Tufts Medicine, Magazine of the Tufts University Medical Alumni Association, Vol. 57, No.3, Summer 1998 Article about humanitarian charitable work of Flights for Humanity, Inc. 501(c)3 non-profit

“Robert Weinberg, Class of 1975,” Technology Review: MIT’s Magazine of Innovation, July-August 1998 Article about humanitarian charitable work of Flights for Humanity, Inc. 501(c)3 non-profit

“Mission of Mercy,” Fitchburg Sentinel & Enterprise newspaper, Vol.158, No.298, October 25, 1998 Article about humanitarian charitable work of Flights for Humanity, Inc. 501(c)3 non-profit

Medical Organization membership (past and present)

College of American Pathologists, Massachusetts College of Emergency Physicians, American College of Emergency Physicians, Massachusetts Medical Society, American Medical Association, American College of Osteopathic Family Practitioners, American Association for the Advancement of Science Institute of Electrical and Electronic Engineers, Biomedical Section

Employment in Clinical Medicine

2000-02 Attending Physician, Emergency Medicine, Jones Memorial Hospital, Wellsville, NY

1997-99 Primary Care Physician, Parkhill Family Practice, Fitchburg, MA

1991-97 Primary Care Physician, Boston Evening Medical Center, Boston, MA

1989-91 Attending Physician, Emergency Medicine, Hale-Haverhill Municipal Hospital, Haverhill MA

1988-89 Attending Physician, Goldberg Medical Associates, Salem, MA

Specific Biomedical Laboratory Skills & Methods cell and tissue culture, necropsies and autopsies, small animal surgery: mice, rats, rabbits, goats

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ELISA assays, RIA assays, radio-iodinating ; cloning genes & rDNA using phage & viral vectors, DNA & RNA isolation & purification; restriction endonuclease fragment analysis & gene mapping, PAGE electrophoresis, column chromatography using gel filtration, ion exchange, affinity columns, Western blotting, DNA/RNA separations using agarose gels, immunoprecipitation techniques, Spectrophotometric assays, Fluorometric assays, Ochterlony gels, immunoelectrophoresis, Immunofluorescent antibody staining, Phase Microscopy, Electron Microscopy, Small animal injections: SQ, IV, IM, IP, Monoclonal antibody production, hybridoma fusions, immunizing animals, Separation, purification & identification of proteins, peptides, nucleic acids

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