SETMAR Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. SETMAR polyclonal antibody Catalog: BS61494 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: munogen and the purity is > 95% (by SDS-PAGE). SETMAR (SET domain and mariner transposase fusion Applications: gene-containing protein), also known as METNASE or WB: 1:500~1:1000 Hsmar1, is a ubiquitously expressed fusion protein with Storage&Stability: histone-lysine N-methyltransferase activity and Store at 4°C short term. Aliquot and store at -20°C long DNA-binding, DNA-looping and DNA-cleavage activi- term. Avoid freeze-thaw cycles. ties. Localizing to the nucleus, SETMAR contains one Specificity: N-terminal SET domain which facilitates the his- SETMAR polyclonal antibody detects endogenous levels tone-lysine methyltransferase activity (at H3-Lys4 and of SETMAR protein. H3-Lys36) and a C-terminal transposase domain which is DATA: responsible for the DNA-binding, -looping and -cleavage activities. Both domains are essential for the proper func- tion of SETMAR. SETMAR specifically functions in DNA repair but, on its own, SETMAR can only bind to 5’-TIR (terminal inverted repeats) in DNA. For interac- tions with non-TIR DNA, SETMAR (via its SET domain) binds to and forms a stable complex with the pre-mRNA processing protein PRP19. Due to alternative splicing Western blot (WB) analysis of SETMAR polyclonal antibody at 1:500 events, two isoforms exist. dilution Product: Lane1:HEK293T whole cell lysate(40ug) Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, Lane2:PC12 whole cell lysate(40ug) 50% glycerol, pH7.2 Lane3:NIH-3T3 whole cell lysate(40ug) Molecular Weight: Note: ~ 78 kDa For research use only, not for use in diagnostic procedure. Swiss-Prot: Q53H47 Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific im- Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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Whole-Genome Microarray Detects Deletions and Loss of Heterozygosity of Chromosome 3 Occurring Exclusively in Metastasizing Uveal Melanoma
Anatomy and Pathology Whole-Genome Microarray Detects Deletions and Loss of Heterozygosity of Chromosome 3 Occurring Exclusively in Metastasizing Uveal Melanoma Sarah L. Lake,1 Sarah E. Coupland,1 Azzam F. G. Taktak,2 and Bertil E. Damato3 PURPOSE. To detect deletions and loss of heterozygosity of disease is fatal in 92% of patients within 2 years of diagnosis. chromosome 3 in a rare subset of fatal, disomy 3 uveal mela- Clinical and histopathologic risk factors for UM metastasis noma (UM), undetectable by fluorescence in situ hybridization include large basal tumor diameter (LBD), ciliary body involve- (FISH). ment, epithelioid cytomorphology, extracellular matrix peri- ϩ ETHODS odic acid-Schiff-positive (PAS ) loops, and high mitotic M . Multiplex ligation-dependent probe amplification 3,4 5 (MLPA) with the P027 UM assay was performed on formalin- count. Prescher et al. showed that a nonrandom genetic fixed, paraffin-embedded (FFPE) whole tumor sections from 19 change, monosomy 3, correlates strongly with metastatic death, and the correlation has since been confirmed by several disomy 3 metastasizing UMs. Whole-genome microarray analy- 3,6–10 ses using a single-nucleotide polymorphism microarray (aSNP) groups. Consequently, fluorescence in situ hybridization were performed on frozen tissue samples from four fatal dis- (FISH) detection of chromosome 3 using a centromeric probe omy 3 metastasizing UMs and three disomy 3 tumors with Ͼ5 became routine practice for UM prognostication; however, 5% years’ metastasis-free survival. to 20% of disomy 3 UM patients unexpectedly develop metas- tases.11 Attempts have therefore been made to identify the RESULTS. Two metastasizing UMs that had been classified as minimal region(s) of deletion on chromosome 3.12–15 Despite disomy 3 by FISH analysis of a small tumor sample were found these studies, little progress has been made in defining the key on MLPA analysis to show monosomy 3.
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SETMAR Antibody
Product Datasheet SETMAR Antibody Catalog No: #43086 Orders: [email protected] Description Support: [email protected] Product Name SETMAR Antibody Host Species Rabbit Clonality Polyclonal Purification Antigen affinity purification. Applications WB IHC Species Reactivity Hu Specificity The antibody detects endogenous levels of total SETMAR protein. Immunogen Type protein Immunogen Description Fusion protein of human SETMAR Target Name SETMAR Other Names Mar1; HsMar1; METNASE Accession No. Swiss-Prot#: Q53H47Gene ID: 6419 Calculated MW 78kd Concentration 1mg/ml Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C Application Details Western blotting: 1:200-1:1000 Immunohistochemistry: 1:20-1:100 Images Gel: 6%SDS-PAGE Lysate: 40 µg Lane: Jurkat cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43086 at dilution 1/20. Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43086 at dilution 1/20. Background This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Note: This product is for in vitro research use only and is not intended for use in humans or animals.
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