Aquaporins: Structure, Systematics, and Regulatory Features A

Total Page:16

File Type:pdf, Size:1020Kb

Aquaporins: Structure, Systematics, and Regulatory Features A Russian Journal of Plant Physiology, Vol. 51, No. 1, 2004, pp. 127–137. Translated from Fiziologiya Rastenii, Vol. 51, No. 1, 2004, pp. 142–152. Original Russian Text Copyright © 2004 by Shapiguzov. REVIEWS Aquaporins: Structure, Systematics, and Regulatory Features A. Yu. Shapiguzov Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276 Russia; fax: 7 (095) 977-9372; e-mail: [email protected] Received May 13, 2003 Abstract—The review describes current views on the molecular structure, systematics, and functional regula- tion of aquaporins. These recently discovered channel proteins play a principal role in water transport across cell membranes in the majority of living organisms. Key words: aquaporins - cell membranes - water relations INTRODUCTION family—is sometimes used for aquaporins in the litera- ture. Multicellular organisms have developed specialized Presently, the number of discovered aquaporins tissues with low hindrance to water flows. Nonetheless, exceeds two hundred, and the plant aquaporins consti- the transfer of water molecules across the cell mem- tute a considerable part of this family [10]. For exam- brane is the main step in water transport [1]. ple, Arabidopsis thaliana genome contains 35 aqua- The ability of cells to control outward or inward porin genes [11–13], and Zea mays contains more than movement of water and solutes is a matter of principal 30 such genes [14]. significance. Cell membranes represent selective com- Water transport is extremely difficult for quantita- plex filters that regulate the transport of ions, organic tive assessment. Unlike the transmembrane ion trans- substances, and water. The current knowledge of mem- port associated with membrane potential changes, brane structure and functions is rapidly expanding [2]. water transport is normally evaluated from the osmoti- A number of transmembrane carrier proteins have been cally induced changes of the cell volume, and such discovered and characterized. However, despite this measurements are often complicated [15]. Further- progress, molecular basis of the transmembrane water more, a background component of water transport is transport remained poorly understood until recently. rather high owing to universal abundance of water and The water permeability of biological membranes was its rapid diffusion through the lipid bilayer. commonly attributed to diffusion of water across the The oocytes of a frog Xenopus played an important lipid bilayer. However, some physiological processes role in studying water carriers [16]. The membranes of are associated with translocation of large amounts of these cells feature a very low intrinsic permeability to water or with rapid changes in membrane permeability water. When mRNA is injected into the oocyte, it is nor- to water. Such phenomena cannot be explained by mally expressed as a functional protein. After the injec- water diffusion across the lipids, which implicates the tion of aqp1 gene mRNA, the water permeability of the existence of water-carrying proteins. oocyte membrane increased manyfold; the cell swelled The first of such carriers was revealed in the mam- rapidly in a hypoosmotic buffer and lysed. By compar- malian erythrocytes whose cell membrane is highly ing the kinetics of cell volume changes in untreated and permeable to water [3]. The initial observation that aqp1-expressing oocytes, it was possible to assess the activity of the protein examined. HgCl2 and organic–mercurial substances inhibit water transport implied the involvement of protein in this pro- Another important approach to studying aquaporins cess [4]. A specific polypeptide was isolated later and is the evaluation of their activity in proteoliposomes termed CHIP28 (channel-like integral protein of 28 kD) [17]. [5, 6]. Several homological proteins were revealed in The investigation of aquaporins led to several other mammalian tissues. One of such proteins, called important conclusions. (1) Many aquaporins signifi- MIP (major intrinsic protein of lens), had been known cantly reduce the activation energy for the transmem- for a long time although its function was unclear [7]. brane water transfer. The rate of water movement Later studies demonstrated the presence and, in some across the channel approaches to the diffusion rate in cases, abundance of homological proteins in many bulk water [17, 18]. (2) Aquaporins provide for bidirec- organisms [8], and their transport activity was proven. tional passage of water, and the transport direction is A term “aquaporins” (Aqp) was adopted, and CHIP28 determined by physical conditions of the medium [19, was renamed as Aqp1 [9]. An alternative term—MIP 20]. (3) Water transport is passive: it does not require 1021-4437/04/5101-0127 © 2004 MAIK “Nauka /Interperiodica” 128 SHAPIGUZOV energy supply, and water moves downhill the gradient proteins is an inherent homology between the halves of of water potential [20]. (4) Aquaporin channels are their molecules. The repeated sequences are oriented in highly selective. The water transport across the cell the same direction. It is likely that the aquaporin gene membrane normally occurs without a concomitant originated from the duplication of a half-sized gene [10]. transfer of any ion species, including protons [21, 22]. Six transmembrane α-helical protein domains form (5) Many aquaporins are sensitive to mercury-contain- a barrel-like configuration in the membrane plane. The ing inhibitors. The mercury atoms bind to conserved amino- and carboxy-terminal domains face the cyto- cysteine residues and are thought to block water pas- plasm and account for a specific regulation of aqua- sage through the channel by steric hindrance [23, 24]. porin activity. One of the cytoplasmic loops and one of There is evidence that mercury induces conformational the periplasmic loops comprise two short α-helical changes in aquaporins [25]. domains on the opposite sides of the “barrel.” These Some aquaporins turned out to be specific not so domains participate in the formation of the water chan- much to water as to glycerol and several other sub- nel. The tops of these domains are located in close stances [26], for example, gases [20, 27]. Some aqua- proximity to each other inside the molecule (Fig. 1). porins, including aquaporins of plant origin, are perme- Each of these tops contains an NPA (Asn-Pro-Ala) able to formamide [28] and urea [29]. The substrate motif, which is conserved for all aquaporins with rare specificity of these proteins in plants is still unknown. exceptions [14, 46]. This type of structure was called There is evidence that aquaporins are permeable to eth- the “hourglass model” [19, 47]. anol and methanol; however, the study of this phenome- When incorporated into the membrane, aquaporins non is highly complicated owing to unhampered diffu- produce homotetramers [48, 49]. Apparently, this sion of alcohols through the lipid bilayer [30]. The aqua- assemblage is required for correct folding and stability porins of Chara corallina are permeable to H2O2 [31]. of the protein, as well as for protein sorting and post- The expression of Aqp1 increases the CO2 perme- translational modifications including glycosylation. ability of the Xenopus oocyte membrane [32], although Each of the four subunits within the complex produces the significance of this property for the mammalian an independent water channel, whereas the pore for the physiology in vivo was questioned in some works [33, aforementioned cGMP-dependent ion transport is ori- 34]. Mercury-containing organic substances inhibit ented along to the tetramer axis (Fig. 2) [39]. The sta- CO2 transport across the plasma membrane in cyano- bility of the quaternary structure varies for different bacteria Synechococcus [35] and in higher plants [36]. phylogenetic clusters of aquaporins: the tetramers of This provides evidence for CO2-transporting activity of aquaporins with glycerol specificity are less stable [50]. aquaporins and suggests their involvement in photosyn- The most complete data about the structure of water thesis; nevertheless, this topic remains poorly understood. channel were obtained after crystallization of Aqp1 It is possible that some aquaporins are permeable to other [51] and its X-ray crystallographic analysis [52, 53]. gases, including O2, NH3, and NO [27]. The attempts to Most likely the structural features discovered for Aqp1 study this issue encounter the problem of high permeabil- are also valid for other water-conducting proteins of the ity of lipids to the substances mentioned [37]. family. A small part of Aqp1 molecules expressed in Xenopus The shape of the aquaporin channel is reminiscent oocytes becomes permeable to Na+ after the intracellular of a dumb-bell (Fig. 3). The channel consists of an injection of cyclic GMP [38]. Apparently, the binding of external and cytoplasmic funnels and a long selective cGMP results in the opening of the cationic channel in pore with connecting them. The funnel surface consists the aquaporin molecule. The formation of aqueous and mainly of polar amino-acid residues. Here the hydro- cationic pores seems to occur in different regions of the philic substrates undergo primary selection and their protein molecule (see below) [39]. The ionic perme- hydration shell is removed at the expense of hydrogen ability was also demonstrated for Aqp0 [40–42], Aqp6 bond formation with the protein atoms. The funnels of [43, 44], and plant aquaporin Nod26 [45]. Further glycerol-specific aquaporins are less
Recommended publications
  • Protein Family Review
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Protein family review The aquaporins comment Elisabeth Kruse, Norbert Uehlein and Ralf Kaldenhoff Address: Institute of Botany, Department of Applied Plant Sciences, Darmstadt University of Technology, Schnittspahnstraße 10, D-64287 Darmstadt, Germany. Correspondence: Ralf Kaldenhoff. Email: [email protected] reviews Published: 28 February 2006 Genome Biology 2006, 7:206 (doi:10.1186/gb-2006-7-2-206) The electronic version of this article is the complete one and can be found online at http://genomebiology.com/2006/7/2/206 © 2006 BioMed Central Ltd reports Summary Water is the major component of all living cells, and efficient regulation of water homeostasis is essential for many biological processes. The mechanism by which water passes through biological membranes was a matter of debate until the discovery of the aquaporin water channels. Aquaporins are intrinsic membrane proteins characterized by six transmembrane helices that deposited research selectively allow water or other small uncharged molecules to pass along the osmotic gradient. In addition, recent observations show that some aquaporins also facilitate the transport of volatile substances, such as carbon dioxide (CO2) and ammonia (NH3), across membranes. Aquaporins usually form tetramers, with each monomer defining a single pore. Aquaporin-related proteins are found in all organisms, from archaea to mammals. In both uni- and multicellular organisms, numerous isoforms have been identified that are differentially expressed and modified by post- translational processes, thus allowing fine-tuned tissue-specific osmoregulation. In mammals, aquaporins are involved in multiple physiological processes, including kidney and salivary gland refereed research function.
    [Show full text]
  • Single Amino Acid Substitutions in the Selectivity Filter
    Ampah-Korsah et al. BMC Plant Biology (2017) 17:61 DOI 10.1186/s12870-017-1009-3 RESEARCHARTICLE Open Access Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable Henry Ampah-Korsah, Yonathan Sonntag, Angelica Engfors, Andreas Kirscht, Per Kjellbom and Urban Johanson* Abstract Background: Aquaporins (AQPs) are integral membrane proteins that facilitate transport of water and/or other small neutral solutes across membranes in all forms of life. The X Intrinsic Proteins (XIPs) are the most recently recognized and the least characterized aquaporin subfamily in higher plants. XIP1s have been shown to be impermeable to water but permeable to boric acid, glycerol, hydrogen peroxide and urea. However, uncertainty regarding the determinants for selectivity and lack of an activity that is easy to quantify have hindered functional investigations. In an effort to resolve these issues, we set out to introduce water permeability in Nicotiana benthamiana XIP1;1α (NbXIP1;1α), by exchanging amino acid residues of predicted alternative aromatic/arginine (ar/R) selectivity filters of NbXIP1;1α for residues constituting the water permeable ar/R selectivity filter of AtTIP2;1. Results: Here, we present functional results regarding the amino acid substitutions in the putative filters as well as deletions in loops C and D of NbXIP1;1α. In addition, homology models were created based on the high resolution X-ray structure of AtTIP2;1 to rationalize the functional properties of wild-type and mutant NbXIP1;1α. Our results favour Thr 246 rather than Val 242 as the residue at the helix 5 position in the ar/R filter of NbXIP1;1α and indicate that the pore is not occluded by the loops when heterologously expressed in Pichia pastoris.
    [Show full text]
  • Combining Genome-Wide and Transcriptome-Wide Analyses Reveal
    Li et al. BMC Genomics (2019) 20:538 https://doi.org/10.1186/s12864-019-5928-2 RESEARCHARTICLE Open Access Combining genome-wide and transcriptome-wide analyses reveal the evolutionary conservation and functional diversity of aquaporins in cotton Weixi Li, Dayong Zhang, Guozhong Zhu, Xinyue Mi and Wangzhen Guo* Abstract Background: Aquaporins (AQPs) are integral membrane proteins from a larger family of major intrinsic proteins (MIPs) and function in a huge variety of processes such as water transport, plant growth and stress response. The availability of the whole-genome data of different cotton species allows us to study systematic evolution and function of cotton AQPs on a genome-wide level. Results: Here, a total of 53, 58, 113 and 111 AQP genes were identified in G. arboreum, G. raimondii, G. hirsutum and G. barbadense, respectively. A comprehensive analysis of cotton AQPs, involved in exon/intron structure, functional domains, phylogenetic relationships and gene duplications, divided these AQPs into five subfamilies (PIP, NIP, SIP, TIP and XIP). Comparative genome analysis among 30 species from algae to angiosperm as well as common tandem duplication events in 24 well-studied plants further revealed the evolutionary conservation of AQP family in the organism kingdom. Combining transcriptome analysis and Quantitative Real-time PCR (qRT-PCR) verification, most AQPs exhibited tissue-specific expression patterns both in G. raimondii and G. hirsutum. Meanwhile, a bias of time to peak expression of several AQPs was also detected after treating G. davidsonii and G. hirsutum with 200 mM NaCl. It is interesting that both PIP1;4 h/i/j and PIP2;2a/e showed the highly conserved tandem structure, but differentially contributed to tissue development and stress response in different cotton species.
    [Show full text]
  • Snapshot: Mammalian TRP Channels David E
    SnapShot: Mammalian TRP Channels David E. Clapham HHMI, Children’s Hospital, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA TRP Activators Inhibitors Putative Interacting Proteins Proposed Functions Activation potentiated by PLC pathways Gd, La TRPC4, TRPC5, calmodulin, TRPC3, Homodimer is a purported stretch-sensitive ion channel; form C1 TRPP1, IP3Rs, caveolin-1, PMCA heteromeric ion channels with TRPC4 or TRPC5 in neurons -/- Pheromone receptor mechanism? Calmodulin, IP3R3, Enkurin, TRPC6 TRPC2 mice respond abnormally to urine-based olfactory C2 cues; pheromone sensing 2+ Diacylglycerol, [Ca ]I, activation potentiated BTP2, flufenamate, Gd, La TRPC1, calmodulin, PLCβ, PLCγ, IP3R, Potential role in vasoregulation and airway regulation C3 by PLC pathways RyR, SERCA, caveolin-1, αSNAP, NCX1 La (100 µM), calmidazolium, activation [Ca2+] , 2-APB, niflumic acid, TRPC1, TRPC5, calmodulin, PLCβ, TRPC4-/- mice have abnormalities in endothelial-based vessel C4 i potentiated by PLC pathways DIDS, La (mM) NHERF1, IP3R permeability La (100 µM), activation potentiated by PLC 2-APB, flufenamate, La (mM) TRPC1, TRPC4, calmodulin, PLCβ, No phenotype yet reported in TRPC5-/- mice; potentially C5 pathways, nitric oxide NHERF1/2, ZO-1, IP3R regulates growth cones and neurite extension 2+ Diacylglycerol, [Ca ]I, 20-HETE, activation 2-APB, amiloride, Cd, La, Gd Calmodulin, TRPC3, TRPC7, FKBP12 Missense mutation in human focal segmental glomerulo- C6 potentiated by PLC pathways sclerosis (FSGS); abnormal vasoregulation in TRPC6-/-
    [Show full text]
  • Study of Selectivity and Permeation in Voltage-Gated Ion Channels
    Study of Selectivity and Permeation in Voltage-Gated Ion Channels By Janhavi Giri, Ph.D. Visiting Research Faculty Division of Molecular Biophysics and Physiology Rush University Medical Center Chicago, IL, USA Email: [email protected] i TABLE OF CONTENTS Page TABLE OF CONTENTS ..................................................................................................... i LIST OF TABLES ............................................................................................................. iii LIST OF FIGURES ........................................................................................................... iv SUMMARY ............................................................................................................... xvi CHAPTER 1. INTRODUCTION ....................................................................................... 1 1.1. Background ................................................................................................... 1 1.2. Overview .................................................................................................... 10 CHAPTER 2. SELF-ORGANIZED MODELS OF SELECTIVITY IN CALCIUM CHANNELS ........................................................................................... 13 2.1. Introduction ................................................................................................ 13 2.2. Methods ...................................................................................................... 19 2.2.1. Model of Channel and Electrolyte ........................................................19
    [Show full text]
  • An Aquaporin-4/Transient Receptor Potential Vanilloid 4 (AQP4/TRPV4) Complex Is Essential for Cell-Volume Control in Astrocytes
    An aquaporin-4/transient receptor potential vanilloid 4 (AQP4/TRPV4) complex is essential for cell-volume control in astrocytes Valentina Benfenatia,1, Marco Caprinib,1, Melania Doviziob, Maria N. Mylonakouc, Stefano Ferronib, Ole P. Ottersenc, and Mahmood Amiry-Moghaddamc,2 aInstitute for Nanostructured Materials, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy; bDepartment of Human and General Physiology, University of Bologna, 40127 Bologna, Italy; and cCenter for Molecular Biology and Neuroscience and Department of Anatomy, University of Oslo, 0317 Oslo, Norway Edited* by Peter Agre, Johns Hopkins Malaria Research Institute, Baltimore, MD, and approved December 27, 2010 (received for review September 1, 2010) Regulatory volume decrease (RVD) is a key mechanism for volume channels (VRAC). Osmolyte efflux through VRAC is thought to control that serves to prevent detrimental swelling in response to provide the driving force for water exit (6, 7). The factors that hypo-osmotic stress. The molecular basis of RVD is not understood. initiate RVD have received comparatively little attention. Here Here we show that a complex containing aquaporin-4 (AQP4) and we test our hypothesis that activation of astroglial RVD depends transient receptor potential vanilloid 4 (TRPV4) is essential for RVD on a molecular interaction between AQP4 and TRPV4. fi in astrocytes. Astrocytes from AQP4-KO mice and astrocytes treated Our hypothesis rests on our recent nding that TRPV4 is with TRPV4 siRNA fail to respond to hypotonic stress by increased strongly expressed in astrocytic endfeet membranes abutting the – intracellular Ca2+ and RVD. Coimmunoprecipitation and immunohis- pia (including the extension of the pia that lines the Virchow tochemistry analyses show that AQP4 and TRPV4 interact and coloc- Robin spaces) and in endfeet underlying ependyma of the ven- alize.
    [Show full text]
  • Genome-Wide Identification, Characterization, and Expression
    www.nature.com/scientificreports OPEN Genome-wide identification, characterization, and expression profile of aquaporin gene family in Received: 23 August 2016 Accepted: 13 March 2017 flax (Linum usitatissimum) Published: 27 April 2017 S. M. Shivaraj1,*, Rupesh K. Deshmukh2,*, Rhitu Rai1, Richard Bélanger2, Pawan K. Agrawal3 & Prasanta K. Dash1 Membrane intrinsic proteins (MIPs) form transmembrane channels and facilitate transport of myriad substrates across the cell membrane in many organisms. Majority of plant MIPs have water transporting ability and are commonly referred as aquaporins (AQPs). In the present study, we identified aquaporin coding genes in flax by genome-wide analysis, their structure, function and expression pattern by pan- genome exploration. Cross-genera phylogenetic analysis with known aquaporins from rice, arabidopsis, and poplar showed five subgroups of flax aquaporins representing 16 plasma membrane intrinsic proteins (PIPs), 17 tonoplast intrinsic proteins (TIPs), 13 NOD26-like intrinsic proteins (NIPs), 2 small basic intrinsic proteins (SIPs), and 3 uncharacterized intrinsic proteins (XIPs). Amongst aquaporins, PIPs contained hydrophilic aromatic arginine (ar/R) selective filter but TIP, NIP, SIP and XIP subfamilies mostly contained hydrophobic ar/R selective filter. Analysis of RNA-seq and microarray data revealed high expression of PIPs in multiple tissues, low expression of NIPs, and seed specific expression of TIP3 in flax. Exploration of aquaporin homologs in three closely relatedLinum species bienne, grandiflorum and leonii revealed presence of 49, 39 and 19 AQPs, respectively. The genome-wide identification of aquaporins, first in flax, provides insight to elucidate their physiological and developmental roles in flax. Water absorption from soil through roots and its translocation to different parts is of paramount importance for innate physiological processes in plants.
    [Show full text]
  • Gadolinium Chloride Restores the Function of the Gap Junctional Intercellular Communication Between Hepatocytes in a Liver Injury
    Article Gadolinium Chloride Restores the Function of the Gap Junctional Intercellular Communication between Hepatocytes in a Liver Injury Le Yang, Chengbin Dong, Lei Tian, Xiaofang Ji, Lin Yang and Liying Li * Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China * Correspondence: [email protected]; Tel.: +0086-10-83950468; Fax: +0086-10-83950468; Received: 3 July 2019; Accepted: 30 July 2019; Published: 31 July 2019 Abstract: Background: Gadolinium chloride (GdCl3) has been reported to attenuate liver injury caused by a variety of toxicants. Gap junctional intercellular communication (GJIC) is thought to be essential in controlling liver homeostasis and pathology. Here we evaluate the effects of GdCl3 on functional GJIC and connexin expression in mouse models and primary hepatocytes. Methods: Mice were administered GdCl3 intraperitoneally the day before a carbon tetrachloride (CCl4) injection or bile duct ligation (BDL) operation. Primary hepatocytes were treated with CCl4 or lipopolysaccharides (LPS), with or without GdCl3. A scrape loading/dye transfer assay was performed to assess the GJIC function. The expression of connexins was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescent staining. Results: CCl4 treatment or the BDL operation led to the dysfunction of GJIC and a down-regulation of Cx32 and Cx26 in injured liver. GdCl3 administration restored GJIC function between hepatocytes by facilitating the transfer of fluorescent dye from one cell into adjacent cells via GJIC, and markedly prevented the decrease of Cx32 and Cx26 in injured liver. In primary hepatocytes, CCl4 or LPS treatment induced an obvious decline of Cx32 and Cx26, whereas GdCl3 pretreatment prevented the down-regulation of connexins.
    [Show full text]
  • Cooperativity in Plant Plasma Membrane Intrinsic Proteins
    www.nature.com/scientificreports OPEN Cooperativity in Plant Plasma Membrane Intrinsic Proteins (PIPs): Mechanism of Increased Water Received: 19 January 2018 Accepted: 10 July 2018 Transport in Maize PIP1 Channels in Published: xx xx xxxx Hetero-tetramers Manu Vajpai , Mishtu Mukherjee & Ramasubbu Sankararamakrishnan Plant aquaporins (AQPs) play vital roles in several physiological processes. Plasma membrane intrinsic proteins (PIPs) belong to the subfamily of plant AQPs. They are further subdivided into two closely related subgroups PIP1s and PIP2s. While PIP2 members are efcient water channels, PIP1s from some plant species have been shown to be functionally inactive. Aquaporins form tetramers under physiological conditions. PIP2s can enhance the water transport of PIP1s when they form hetero- tetramers. However, the role of monomer-monomer interface and the signifcance of specifc residues in enhancing the water permeation of PIP1s have not been investigated at atomic level. We have performed all-atom molecular dynamics (MD) simulations of homo-tetramers and four diferent hetero- tetramers containing ZmPIP1;2 and ZmPIP2;5 from Zea mays. ZmPIP1;2 in a tetramer assembly will have two interfaces, one formed by transmembrane segments TM4 and TM5 and the other formed by TM1 and TM2. We have analyzed channel radius profles, water transport and potential of mean force profles of ZmPIP1;2 monomers. Results of MD simulations clearly revealed the infuence of TM4-TM5 interface in modulating the water transport of ZmPIP1;2. MD simulations indicate the importance of I93 residue from the TM2 segment of ZmPIP2;5 for the increased water transport in ZmPIP1;2. Plant aquaporins constitute an important component in the superfamily of Major Intrinsic Proteins (MIPs)1,2.
    [Show full text]
  • Characterization of Membrane Proteins: from a Gated Plant Aquaporin to Animal Ion Channel Receptors
    Characterization of Membrane Proteins: From a gated plant aquaporin to animal ion channel receptors Survery, Sabeen 2015 Link to publication Citation for published version (APA): Survery, S. (2015). Characterization of Membrane Proteins: From a gated plant aquaporin to animal ion channel receptors. Department of Biochemistry and Structural Biology, Lund University. Total number of authors: 1 General rights Unless other specific re-use rights are stated the following general rights apply: Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Read more about Creative commons licenses: https://creativecommons.org/licenses/ Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. LUND UNIVERSITY PO Box 117 221 00 Lund +46 46-222 00 00 Printed by Media-Tryck, Lund University 2015 SURVERY SABEEN Characterization of Membrane Proteins - From a gated plant aquaporin to animal ion channel
    [Show full text]
  • Labned.Com Bovine
    Antibodies Predicted to Bind Homologous Bovine Targets This is a catalog of all antibodies predicted to react with bovine proteins in addition to their validated reactivities. This catalog was assembled by identifying proteins targeted by LabNed antibodies that share 99% sequence homology withtheir bovine counterparts. Table of Contents High Affinity Antibodies .................................................................................... 1 High Sensitivity ELISA Kits .................................................................................... 31 High Affinity Antibodies LabNed supplies only the highest quality antibodies. Our high-affinity polyclonal and monoclonal antibodies are thoroughly validated by Western Blotting, Immunohistochemistry and ELISA. This is our comprehensive catalog of our antibody products predicted to react with bovine proteins, sorted in alphabetical order by target gene name. Applications Gene Name Product Name Reactivity WB ABAT Anti-ABA Human, Mouse, Rat WB ABCA1 Anti-ABCA1 Human, Mouse, Rat WB ABCA1 Anti-ABCA1 Human, Mouse, Rat IHC-P, WB ABCB11 Anti-ABCB11 Human, Mouse, Rat IHC-P, WB ABCC1 Anti-MRP1 Human, Mouse, Rat WB ABCC4 Anti-MRP4 Human, Mouse, Rat IHC-P, WB ABCD3 Anti-PMP70 Human, Mouse, Rat WB ABCE1 Anti-ABCE1 Human, Mouse, Rat WB ABCG1 Anti-ABCG1 Human, Mouse, Rat WB ABCG2 Anti-ABCG2 Human, Mouse, Rat WB ABCG4 Anti-ABCG4 Human, Mouse, Rat WB ABCG5 Anti-ABCG5 Human IHC-P, ICC, WB ABI1 Anti-ABI1 Human, Mouse, Rat WB ABI2 Anti-ABI2 Human, Mouse, Rat WB ACO2 Anti-Aconitase 2 Human, Mouse, Rat
    [Show full text]
  • Transyt, the Transport Systems Tracker
    bioRxiv preprint doi: https://doi.org/10.1101/2021.04.29.441738; this version posted April 30, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. TranSyT, the Transport Systems Tracker Davide Lagoa1,2, José P. Faria2, Filipe Liu2, Emanuel Cunha1, Christopher S. Henry2 and Oscar Dias1,* 1 Centre of Biological Engineering, University of Minho, Braga, 4704-553, Portugal 2 Computing, Environment, and Life Sciences Division, Argonne National Laboratory, Lemont, IL 60439, USA * To whom correspondence should be addressed. Tel: +351 253 604422; Fax: ; Email: [email protected] ABSTRACT The importance and rate of development of GSM models have been growing for the last years, increasing the demand for software solutions that automatise several steps of this process. However, since TRIAGE’s release, software development for automatic integration of transport reactions into models has stalled. Here we present the Transport Systems Tracker (TranSyT), the next iteration of TRIAGE. Unlike its predecessor, TranSyT does not rely on manual curation to expand its internal database, derived from highly-curated records retrieved from TCDB, and complemented with information from other data sources. TranSyT compiles information regarding, TC families, transport proteins, and derives reactions into its internal database, making it available for rapid annotation of complete genomes. All transport reactions have GPR associations and can be exported with identifiers from four different metabolite databases. TranSyT is currently available as a plugin for merlin v4.0 and an app for KBase.
    [Show full text]