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(10) Patent No.: US 7857978 B2

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(10) Patent No.: US 7857978 B2 US007857978B2 (12) United States Patent (10) Patent No.: US 7,857,978 B2 Jensen et a]. (45) Date of Patent: Dec. 28, 2010 (54) MEMBRANE FOR FILTERING OF WATER (58) Field of Classi?cation Search .......... .. 210/ 500.35, 210/500.36, 500.42, 641, 321.75, 257.2, (75) Inventors: Peter Holme Jensen, Copenhagen (DK); 210/500.2, 7, 490, 6504653, 655, 500.1, Danielle Keller, Odense (DK); Claus 210/500.29, 900; 435/17.1, 4; 436/524, 436/172, 82.05; 530/402; 425/450, 1.21, HéliX Nielsen, Taastrup (DK) 425/9, 32, 417, 489; 264/4.1, 4.3, 4.6, 41; 424/450; 977/7l3i7l4, 718; 422/101 (73) Assignee: Aquaporin A/S, Copehhagen N (DK) See application ?le for complete search history. ( * ) Notice: Subject to any disclaimer, the term of this (56) References Cited patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days. U.S. PATENT DOCUMENTS 3,906,250 A 9/1975 Loeb (21) Appl. No.: 11/915,121 (Continued) (22) PCT Filed: May 19, 2006 FOREIGN PATENT DOCUMENTS EP 1885477 Bl 2/2010 (86) PCT No.: PCT/DK2006/000278 W0 WO 02/13955 2/2002 W0 WO 2004/099088 11/2004 § 371 (0X1)’ W0 WO 2007/033675 3/2007 (2), (4) Date: May 12, 2008 OTHER PUBLICATIONS Coury et al., Reconstintution of Water channel function of aquaporins (87) PCT Pub. No.: WO2006/122566 l and 2 by expression in yeast secretory vesicles, 1998, The American Physiological Society, pp. F34 -F42 .* PCT Pub. Date: Nov. 23, 2006 (Continued) (65) Prior Publication Data Primary ExamineriTony G Soohoo Assistant ExamineriDavid C Mellon US 2009/0120874 A1 May 14, 2009 (74) Attorney, Agent, or FirmiKristina Bieker-Brady; Clark & Elbing LLP Related U.S. Application Data (57) ABSTRACT (60) Provisional application No. 60/ 683,466, ?led on May Disclosed are novel Water membranes comprising lipid bilay 20, 2005, provisional application No. 60/718,890, ers incorporating functional aqua-porins. The lipid bilayers ?led on Sep. 20, 2005. are arranged in sandwich structures including hydrophilic or hydro-phobic support materials. Also disclosed are Water (30) Foreign Application Priority Data puri?cation devices/systems, including reverse osmosis ?l tering devices that include membranes having functional May 20, 2005 (DK) ....................... .. PA 2005 00740 aquaporins. Methods of Water puri?cation and methods of Sep. 20, 2005 (DK) ....................... .. PA 2005 01309 preparing the membranes are also disclosed. Further, the invention provides for a neW type of perforated, hydrophobic (51) Int. Cl. polymer ?lm and to membranes containing lipid bilayers B01D 69/02 (2006.01) having other transmembrane proteins than aquaporins intro (52) U.S. Cl. ..................... .. 210/652; 210/650; 210/651; duced. 210/653; 210/655; 210/500.1; 210/500.29; 210/641; 210/321.75; 422/101; 424/450 46 Claims, 14 Drawing Sheets Supported lipid bilayer with incorporated Aquaporln molecules Porous support of llpirl lrilayer, like mica, nurscovlte, mica time . polyeulfon, M0,, oellulose or other sunnort with IIWII'OIJIIIIIC surface. Planar liniil bilayer membrane with Incorporated mumporlns. {if-l Auuaporinmulecule n Plrospholipill molecule or other :unplu'philic lipid molecule US 7,857,978 B2 Page 2 US. PATENT DOCUMENTS Ilan et al., “The Mechanism of Proton Exclusion in 4,193,267 A 3/1980 Loeb Aquaporin Channels,” PROTEINS: Structure, Function, and 4,966,708 A 10/1990 Oklejas et a1. Bioinformatics 551223-228 (2004). 5,741,416 A 4/1998 Tempest, Jr. Jensen et al., “Electrostatic Tuning of Permeation and Selec 6,297,059 B1* 10/2001 Song et a1. ................ .. 436/501 tivity in Aquaporin Water Channels,” Biophys. .1. 8512884 7,563,370 B2 7/2009 Thorsen et a1. 2899 (2003). 7,566,402 B2 7/2009 Thorsen et a1. Leonenko et al., “Supported Planar Bilayer Formation by 7,713,544 B2* 5/2010 Chaikofet a1. ............ .. 424/450 Vesicle Fusion: The Interaction of PhospholipidVesicles With 2001/0034432 A1* 10/2001 Sodroskiet a1. ........... .. 530/350 Surfaces and the Effect of Gramicidin on Bilayer Properties 2002/0107215 A1* 8/2002 Brown et a1. ..... .. 514/44 Using Atomic Force Microscopy,” Biochim. Biophys. Acta 2003/0102263 A1 * 6/2003 Lopez et a1. .............. .. 210/639 2004/0049230 A1 3/2004 Montemagno et a1. 1509:131-147 (2000). 2007/0087328 A1* 4/2007 Sleytr et a1. .................. .. 435/4 Lin et al., “Amyloid [3 Protein Forms Ion Channels: Implica 2007/0275480 A1 * 11/2007 Brander et a1. ............ .. 436/501 tions for AlZheimer’s Disease Pathophysiology,” FASEB .1. 2009/0007555 A1 1/2009 Jensen 15:2433-2444 (2001). 2009/0120874 A1 5/2009 Jensen et a1. Montal et al., “Formation of Biomolecular Membranes from 2010/0178592 A1 * 7/2010 Cinquin et a1. ............ .. 429/512 Lipid Monolayers and a Study of Their Electrical Properties,” Proc. Nat. Acad. Sci. USA. 69:3561-3566 (1972). OTHER PUBLICATIONS Murata et al., “Structural Determinants of Water Permeation Saparov et al., Water and Ion Permeation of Aquaporin-l in Through Aquaporin-l,” Nature 407:599-605 (2000). Planar Lipid Bilayers, Jun. 15, 2001, The Journal of Biologi Pohl et al., “The Effect of a Transmembrane Osmotic Flux on cal Chemistry, pp. 31515-31520.* the Ion Concentration Distribution in the Immediate Mem Becker et al., The World of the Cell, 2006, Pearson Benjamin brane Vicinity Measured by Microelectrodes,” Biophys. .1. Cummings, Sixth Edition, pp. 171-174,203.* 721171 1-1718 (1997). Tien et al., Planar Lipid Bilayers (BLMs) and their Applica Pohl et al., “Highly Selective Water Channel Activity Mea tions, 2003, Elsevior, Membrane Science and Technology sured by Voltage Clamp: Analysis of Planar Lipid Bilayers Series 7, pp. 381-382, 450-454, 807-819, 825-829.* Reconstituted With Puri?ed AqpZ,” Proc. Natl. Acad. Sci. Heyse et al.,“Emerging Techniques for Investigating Molecu USA. 98:9624-9629 (2001). lar Interactions at Lipid Membranes,” Biochimica et Preston et al., “Appearance of Water Channels in Xenopus Biophysica Acta. Mr. Reviews on Biomembranes, Oocytes Expressing Red Cell CHIP28 Protein,” Science 1376(3):319-338 (1998). 256:385-387 (1992). Mou et al., “Gramicidin A Aggregation in Supported Gel Reimhult et al., “Intact Vesicle Adsorption and Supported State Phosphatidylcholine Bilayers,” Biochemistry, 3513222 Biomembrane Formation from Vesicles in Solution: In?u 3226 (1996). ence of Surface Chemistry, Vesicle SiZe, Temperature, and Reviakine I., Brisson A., “Formation of Supported Osmotic Pressure,” Langmuir 19:1681-1691 (2003). Phospholipid Bilayers from Unilamellar Vesicles Investi Ren et al., “Visualization of Water-Selective Pore by Electron gated by Atomic Force Microscopy,” Langmuir, 1611806 Crystallography in Vitreous Ice,” Proc. Natl. Acad. Sci. US. 1815 (2000). A. 98:1398-1403 (2001). Agre et al., “The Aquaporins, Blueprints for Cellular Plumb Rinia et al., “Visualization of Highly Ordered Striated ing Systems,” .1. Biol. Chem. 273:14659-14662 (1998). Domains Induced by Transmembrane Peptides in Supported Borgnia et al., “Cellular and Molecular Biology of the Phosphatidylcholine Bilayers,” Biochemistry 39:5852-5858 Aquaporin Water Channels,” Annu. Rev. Biochem. 68:425 (2000). 458 (1999). Saparov et al., “Water and Ion Permeation of Aquaporin-l in Brian et al., “Allogeneic Stimulation of Cytotoxic T Cells by Planar Lipid Bilayers,” .1. Biol. Chem. 276:31515-31520 Supported Planar Membranes,” Proc. Natl. Acad. Sci. USA. (2001). 81:6159-6163 (1984). Simonsen et al., “Structure of Spin-Coated Lipid Films and Burykin et al., “What Really Prevents Proton Transport Domain Formation in Supported Membranes Formed by through Aquaporin? Charge Self-Energy versus Proton Wire Hydration,” Langmuir 20:9720-9728 (2004). Proposals,” Biophys. .1. 85:3696-3706 (2003). Sui et al., “Structural Basis of Water-Speci?c Transport Chakrabarti et al., “Molecular Basis of Proton Blockage in Through the AQPl Water Channel,” Nature 414:872-878 Aquaporins,” Structure 12:65-74 (2004). (2001). Dainty et al., “Unstirred Layers’ in Frog Skin,” .1. Physiol. Tajkhorshid et al., “Control of the Selectivity of the 182166-78 (1966). Aquaporin Water Channel Family by Global Orientation Tun de Groot et al., “Water Permeation Across Biological Mem ing,” Science 296:525-530 (2002). branes: Mechanism and Dynamics of Aquaporin-l and Tokumasu et al., “Nanoscopic Lipid Domain Dynamics GlpF,” Science 294:2353-2357 (2001). Revealed by Atomic Force Microscopy,” Biophys. .1. de Groot et al., “The Mechanism of Proton Exclusion in the 84:2609-2618 (2003). Aquaporin-l Water Channel,” .1. Mol. Biol. 333:279-293 van Kan et al., “The Peptide Antibiotic Clavanin A Interacts (2003). Strongly and Speci?cally With Lipid Bilayers,” Biochemistry Fettiplace et al., “Water Permeability of Lipid Membranes,” 42:11366-11372 (2003). Physiological Reviews 601510-550 (1980). Webber et al., “Hydrodynamic Studies of Adsorbed Diblock Fu et al., “Structure of a Glycerol-Conducting Channel and Copolymers in Porous Membranes,” Macromolecules the Basis for Its Selectivity,” Science 290:481-486 (2000). 23:1026-1034 (1990). Heymann et al., “Aquaporins: Phylogeny, Structure, and Zeidel et al ., “Reconstitution of Functional Water Channels in Physiology of Water Channels,” News Physiol. Sci. 14: 187 Liposomes Containing Puri?ed Red Cell CHIP28 Protein,” 1 93 (1 999). Biochemistry 31 17436-7440 (1992). US 7,857,978 B2 Page 3 Zhu et al., “Theory and Simulation of Water Permeation in Written Opinion of the International Searching Authority Aquaporin-1,”Bi0phys_ J, 8650-57 (2004), from PCT/DK2006/000278, completed Jan. 8, 2007, com International Search Report from PCT/DK2006/000278, Pleted 12111293001 Completed Jan 8 2007 mailed Jan 29 2007_ Thesis of Danielle Keller, “Chapter 4: Reconstitution of International Preliminary Report on Patentability from PCT/ Cytochrome C oxidase’” pp’ 41-45’ 2005' DK2006/000278, completed Aug. 23, 2007. * Cited by examiner US. Patent Dec. 28, 2010 Sheet 2 0f 14 US 7,857,978 B2 N.22 !_=,... EEEWEWEWEEEEmc??EmEEm2_._Qm_uEEEamzgq225mg? EEEEmEEEE-22-52.5.22:EEEEEE2:62:25.5 522EE==E=EnH2.222555um?higg.FEEmP?EEmEH .EEEEEEEEmE@2285: 2.522::tEEEE US.
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