Superior Confi dence in Hormone Analysis AbsoluteIDQ® Stero17 Kit

KIT

Ready-to-Use

Comprehensive Coverage

Best Fit to Physiological Range

Superior Data Quality

Steroid Hormone Profiling by Mass Spectrometry (LC-MS)

For research use only. Not for use in diagnostic procedures. Key Benefi ts The Most Accurate Steroid Hormone Analysis

Steroid hormones are a group of metabolites that are derived from . They are produced primarily by adrenal glands, testes, and ovaries. Based on their receptor, steroid hormones are classifi ed into fi ve groups: , , , , and . Steroid hormones exert multiple biological functions, including regulation of glucose levels, cell growth, and proliferation. The trend in steroid analysis is moving from measuring single or small sets of steroid hormones with immunoassays towards broader, mass spectrometry-based quantifi cation of steroid hormones (Jeanneret et al., J Chromatogr A 2016). The AbsoluteIDQ® Stero17 Kit is designed to help researchers to unravel the complexities of steroid hormone effects. It provides highly repro- ducible data on 17 functionally important steroid hormones in a single assay.

Ready-to-Use Simple, Guided, Automated Workfl ow • All in one box: no method or reagent • Workfl ow manager guided procedure development required for enhanced process safety KIT • Implemented within one day • Automated quantifi cation and technical validation • For multiple (U)HPLC-MS platforms

High Throughput Superior Data Quality • Only 7 minutes per sample • Quantitative results • Up to 500 samples per week and instrument • Excellent accuracy and precision • Regularly proven in profi ciency tests

Best Fit to Physiological Range Comprehensive Coverage • Refl ection of actual steroid hormone status • Quantifi cation of 17 steroid hormones • Designed to cover physiological range for the • All fi ve steroid hormone classes covered widest range of conditions

Key Features Quantifi cation of 17 Steroid Hormones from all Five Classes

Metabolite Coverage

Gluco corticoids Mineralo corticoids Androgens • • • • • • • 11-Deoxycortisol • 11-Deoxycorticosterone (DOC) • (DHEA) • Dehydroepiandrosterone sulfate (DHEA-S) Progestogens Estrogens • (DHT) • 17α-Hydroxyprogesterone (17α-OHP) • (E2) • • • (E1) •

Kit Components

Reagents & Consumables Methods & Protocols Workfl ow Manager MetIDQ™ • 96-well SPE plate • Sample preparation protocol • Process guidance • Internal & calibration standards • Instrument-specifi c acquisition & • Automated technical validation • Quality controls quantifi cation methods • Basic statistics (optional) • Testmix • System suitability test

Instrument Platforms*

• Waters Xevo TQ-S UHPLC • Waters Xevo TQ-S micro UHPLC • SCIEX 5500 HPLC

* not suited for nano or micro fl ow systems Simple, Guided, Automated Workfl ow Standardized and Automated Workfl ows Designed for High Throughput and Robustness

Sample Registration Sample Measurement Technical Validation Statistics • Register samples • Run samples with LC-MS • Perform automated sensitivity and • Perform tests with MetIDQ™ StatPack Module • Defi ne plate layout • Instrument-specifi c acquisition accuracy check • Use statistical programs of your choice • Generate worklist methods are provided • Inter-plate monitoring of QC results • On demand data analysis by Biocrates and precision

30 min 12 - 32 h 15 min (depending on LC-MS system)

Workflow Manager MetIDQ™ optional Total: 19-40 h for up to 82 samples

5-6 h 1 h 15 min

Sample Preparation Data Quantifi cation Data Evaluation & Biological • Perform system suitability test Export Interpretation • Prepare kit (loading of samples) • Normalize data (optional) • HMDB • Export results in various fi le formats • Consult Biocrates for support

Plate Layout Easy to Use 96-Well Format, for up to 82 Samples

1 2 3 4 5 6 7 8 9 10 11 12 Internal standards (ISTDs) A B Blank: no sample, no ISTDs C D Zero sample: no sample, only ISTDs E F Sample G H 7-point calibration standards

AbsoluteIDQ® Stero17 Kit plate Quality control in low, medium, high concentration

Superior Data Quality Proven Accuracy and Reproducibility

The AbsoluteIDQ® Stero17 Kit provides highly Figure: Mean accuracy robust, quantitative data with excellent accuracy of 7 steroid hormone and precision, as well as reproducibility (Koal levels from quaterly DGKL profi ciency tests et al., J Steroid Biochem Mol Biol 2012). (2011-2017) based on The outstanding kit performance is proven in 48 samples in total. The orange bars periodical profi ciency tests organized by indicate the acceptance the German Society of Clinical Chemistry and range (55%-145%) Laboratory Medicine (DGKL), demonstrated in and the error bars the fi gure to the right. represent ±SD. Best Fit to Physiological Range Capture the Broadest Steroid Hormone Profi le

The serum concentrations of steroid hormones vary considerably depending on age, gender, and health status. The AbsoluteIDQ® Stero17 Kit has been developed to provide highly accurate results that fi t the expected physiological ranges (see table below). For more details, please contact us or refer to the respective client note.

Steroid Calibration Standard Calibration Range Hormone Cal 1-7 [ng/mL] Cal 1 Cal 2 Cal 3 Cal 4 Cal 5 Cal 6 Cal 7 Cortisol 1.0 – 1000 Gluco- Cortisone 0.10 – 100 corticoids 11-Deoxycortisol 0.010 – 10

Aldosterone < 0.025 – 5.0 Physiological range in human Mineralo- Corticosterone 0.030 – 30 serum, according to Human corticoids Metabolome Database DOC < 0.0075 – 15 (HMDB), May 2017 17α-OHP 0.050 – 50 Progestogens Progesterone 0.060 – 9.0 Estradiol < 0.005 – 20 Estrogens Physiological range in Estrone < 0.030 – 15 human serum, according to Biocrates in-house database, Androstenedione 0.032 – 8.0 March 2017 Androsterone < 0.050 – 5.0 DHEA 0.12 – 30 < < Androgens DHEA-S 32 – 8000 Physiological concentration in DHT < 0.012 – 30 human serum may be below Etiocholanolone < 0.060 – 6.0 LLOQ, according to HMDB May 2017 or Biocrates in- Testosterone 0.010 – 10 house database, March 2017

How to Get Started Global Access to High Quality Targeted Metabolomics Results

Your Laboratory Collaborating Laboratory Metabolic Phenotyping Services You have the right instrumentation to run the kit? You want to run the kit in a collaborating laboratory? You want us to measure your samples?  Please contact us for a detailed list of require-  Please contact us, and we will help you to fi nd  Biocrates can perform the full kit workfl ow, as ments and a quote. the right partner within a global network of >100 well as additional services, with short turnaround established laboratories. times. Please contact us for further information.

Related Products AbsoluteIDQ® Stero17 Kit AbsoluteIDQ® Stero17 Kit HPLC Column AbsoluteIDQ® Stero17 Kit HPLC Precolumn AbsoluteIDQ® Stero17 Kit HPLC Setup Box AbsoluteIDQ® Stero17 Kit UHPLC Column AbsoluteIDQ® Stero17 Kit UHPLC Precolumn

® AbsoluteIDQ Stero17 Kit UHPLC Setup Box Document Nr. 35 029, V02-2019 MetIDQ™ StatPack Module Photos: Biocrates, Fotolia, Depositphotos

Data Analysis Service For research use only. Not for use in diagnostic procedures. For USA and Canada: License pending

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