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Molecular Phylogenetic Studies in the Genus Amanita
1170 Molecular phylogenetic studies in the genus Amanita I5ichael Weiß, Zhu-Liang Yang, and Franz Oberwinkler Abstracl A group of 49 Amanita species that had been thoroughly examined morphologically and amtomically was analyzed by DNA sequence compadson to estimate natural groups and phylogenetic rclationships within the genus. Nuclear DNA sequences coding for a part of the ribosomal large subunit were determined and evaluated using neighbor-joining with bootstrap analysis, parsimony analysis, conditional clustering, and maximum likelihood methods, Sections Amanita, Caesarea, Vaginatae, Validae, Phalloideae, and Amidella were substantially confirmed as monophyletic groups, while the monophyly of section Lepidell.t remained unclear. Branching topologies between and within sections could also pafiially be derived. Stbgenera Amanita an'd Lepidella were not supported. The Mappae group was included in section Validae. Grouping hypotheses obtained by DNA analyses are discussed in relation to the distribution of morphological and anatomical chamcters in the studied species. Key words: fungi, basidiomycetes phylogeny, Agarrcales, Amanita systematics, large subunit rDNA, 28S. R6sum6 : A partir d'un groupe de 49 esp,ces d'Amanita prdalablement examinees morphologiquement et anatomiquement, les auteurs ont utilisd la comparaison des s€quences d'ADN pour ddfinir les groupes naturels et les relations phylog6ndtiques de ce genre. Les sdquences de I'ADN nucl6aire codant pour une partie de la grande sous-unit6 ribosomale ont 6t6 ddterminEes et €valu6es en utilisant l'analyse par liaison en lacet avec le voisin (neighbor-joining with bootstrap), l'analyse en parcimonie, le rcgroupement conditionnel et les m€thodes de ressemblance maximale. Les rdsultats confirment substantiellement les sections Afiarira, Caesarea, Uaqinatae, Ualidae, Phalloideae et Amidella, comme groupes monophyldtiques, alors que la monophylie de la section Lepidella demerxe obscure. -
Since 2008, the Small Alaskan
View of the Girdwood ski area from the Alyeska Highway. Steve Trudell, Burke Museum Herbarium, University of Washington ince 2008, the small Alaskan ski Arm Mycological Society (TAMS). educational mushroom walks (including town of Girdwood, located 35 miles TAMS, whose motto appears in the title one for kids led by Girdwood’s local southeast of Anchorage on the of this article, came into being in January, 10-year-old MykoKid [and TAMS Snorth side of Turnagain Arm (the narrow 2017. Its founding co-Presidents are co-President], Gabriel Wingard) that west-east-trending body of water that Kate Mohatt and Gabriel Wingard and are so popular that most fill up as soon separates the northern Kenai Peninsula membership has quickly grown to over as online registration opens, a silent from the main mass of Alaska), has 60 people, not a huge number by Pacific auction to support local non-profit hosted an annual Fungus Fair. Having Northwest mushroom-club standards, organizations such as the Girdwood helped with eight of the ten, I thought it but a great start. Trails Committee, Health Clinic, Center was time to call attention to this fun little Although the Fungus Fair has for Visual Arts, and Skate Park, and an event held in a majestic northern setting. changed over time, regular activities evening social event, held this year at Plus, this year’s 10th Fair was special, not have included an increasingly tasteful the new Girdwood Brewing Company only because of the landmark anniversary, display of locally collected mushrooms (also the site of TAMS membership but also for being the first that involved displayed with classy name tags in beds meetings where weighty fungal matters the membership of the newly formed of vibrant green moss and conifer duff, are discussed over fine craft beers). -
Pipestem Foray Overview
Volume 49:1 January ⁄ February 2008 www.namyco.org Pipestem Foray Overview An East-Coaster’s Perspective A West-Coaster’s Perspective by Dave Wasilewski by Debbie Viess For about 25 years now I have As Steve Trudell rightly pointed out hunted and studied wild mush- to me, don’t gloat about your mush- rooms, but I’ve never been active in rooms until they are safely in your a club. The NAMA Orson K. Miller basket! The continuing “Curse of Memorial Foray held in Pipestem, NAMA” (some call it global warm- WV, this past August was the first ing) slipped in the back door, behind such event that I have ever at- the earlier and heartening West tended. Virginia thunderstorms. Extreme I must admit that, as I drove heat and lack of rain for the previ- south on Interstate 81 through two ous couple of weeks made condi- solid hours of Pennsylvania rainfall tions on the ground challenging for on an eight-hour trip to a place hopeful finders of fungi. Chlorosplenium aeruginascens, one of where little or no rain had fallen for Luckily, my Southern Belle the many delights found at Pipestem. over a week, for the purpose of hostess with the mostest, Coleman hunting wild mushrooms, I felt a bit McCleneghan, took me on a few names like Gyroporus and Pulvero- conflicted. My mind wandered pre-NAMA forays in Virginia, where boletus, tucked among the through conifer groves in the conditions were much improved. My many shades of forest green and Poconos where imaginary boletes very first walk ever along the brown. -
Insect and Disease Observations — May 2020
Vermont Forest Health Insect and Disease Observations — May 2020 Department of Forests, Parks & Recreation May 2020 vtforest.com Weather Recap This month’s weather started off cold and wet, with snowfall occurring in most parts of the state through mid-May. Compared to last year, this month was warmer and dryer than May of 2019. Statewide tem- peratures averaged 52.9°F, which was 2 degrees warmer than May of last year. Statewide, precipitation averaged 2.93 inches, which is 2.73 inches less than May of last year. Minimum relative humidity (RH) values for the Northeast Kingdom were below normal. On May 23rd and 24th, the low RH at the Nulhegan remote automatic weather station was 12%, and Temperature and precipitation departure from the low RH in Montgomery and Walden normal. Maps and data: Northeast Regional Cli- was 8%. This, combined with no precipi- mate Center. tation since May 16th, caused extremely dry fuel conditions and resulted in several fires. Soil moisture drought indices are approach- ing mid-summer values and large dead fuels are at the seasonal minimum. This spring has delayed green- up in most parts of the state, with many species breaking buds later in the month. Photo credit: FPR Staff. Sugar maple phenology at our long-term monitoring site in Underhill had observed bud break on May 11th, with full leaf-out following two weeks later on May 26th. Each date is 8 days later than the long-term average. Some parts of the state experienced frost on May 11th and 12th, which damaged understory sugar maple. -
Mushroom Toxins & Poisonings in New Jersey
Mushroom Toxins & Poisonings in New Jersey & Nearby Eastern North America What this document doesn’t do: (1) This document is not intended to be used as a guide for treatment and should not be so used. (2) Mushrooms should not be selected for eating based on the content of this document. [In identifying mushrooms in poisoning cases, this document does not replace expertise that should be obtained by calling NJPIES and obtaining contact with an experienced mycologist.] (3) This document is not a replacement for a detailed toxicological review of the subject of mushroom poisoning. (4) This document is intended for use with a broad set of audiences; for this reasons, it should not be used uncritically in setting protocols [for example, carrying out a Meixner test would be inappropriate for a first responder who would appropriately focus on collecting a poi- soning victim, the relevant objects from the scene of the poisoning, and the critical timing characteristics of the event such as the delay between ingestion and onset of symptoms.] POISON CONTROL: New Jersey “Poison Control” is called NJPIES (New Jersey Poison Information & Education System). Telephone: 1-800-222-1222 [works in all states—(WARN- ING) WILL CONNECT TO A MOBILE PHONE’S HOME STATE—IF YOU’RE UNCERTAIN, USE A LAND- LINE] If the victim is unconscious, call “911.” Background of these notes: This document was originally compiled by Rod Tulloss and Dorothy Smullen for an NJ Mycol. Assoc. workshop, 25 March 2006. Version 2.0 was compiled by Tulloss. When viewed with Acrobat Reader, underlined red or gray words and phrases are “hot linked cross-references.” We have included a few notes on fungal poisons that are not from “mushrooms.” The notes were prepared by mycologists with experience in diagnosis of fungi involved in cases in which ingestion of toxic fungi was suspected. -
A Case of the Yellow Morel from Israel Segula Masaphy,* Limor Zabari, Doron Goldberg, and Gurinaz Jander-Shagug
The Complexity of Morchella Systematics: A Case of the Yellow Morel from Israel Segula Masaphy,* Limor Zabari, Doron Goldberg, and Gurinaz Jander-Shagug A B C Abstract Individual morel mushrooms are highly polymorphic, resulting in confusion in their taxonomic distinction. In particu- lar, yellow morels from northern Israel, which are presumably Morchella esculenta, differ greatly in head color, head shape, ridge arrangement, and stalk-to-head ratio. Five morphologically distinct yellow morel fruiting bodies were genetically character- ized. Their internal transcribed spacer (ITS) region within the nuclear ribosomal DNA and partial LSU (28S) gene were se- quenced and analyzed. All of the analyzed morphotypes showed identical genotypes in both sequences. A phylogenetic tree with retrieved NCBI GenBank sequences showed better fit of the ITS sequences to D E M. crassipes than M. esculenta but with less than 85% homology, while LSU sequences, Figure 1. Fruiting body morphotypes examined in this study. (A) MS1-32, (B) MS1-34, showed more then 98.8% homology with (C) MS1-52, (D) MS1-106, (E) MS1-113. Fruiting bodies were similar in height, approxi- both species, giving no previously defined mately 6-8 cm. species definition according the two se- quences. Keywords: ITS region, Morchella esculenta, 14 FUNGI Volume 3:2 Spring 2010 MorchellaFUNGI crassipes Volume, phenotypic 3:2 Spring variation. 2010 FUNGI Volume 3:2 Spring 2010 15 Introduction Materials and Methods Morchella sp. fruiting bodies (morels) are highly polymorphic. Fruiting bodies: Fruiting bodies used in this study were collected Although morphology is still the primary means of identifying from the Galilee region in Israel in the 2003-2007 seasons. -
Minnesota Harvester Handbook
Minnesota Harvester Handbook sustainable livelihoods lifestyles enterprise Minnesota Harvester Handbook Additonal informaton about this resource can be found at www.myminnesotawoods.umn.edu. ©2013, Regents of the University of Minnesota. All rights reserved. Send copyright permission inquiries to: Copyright Coordinator University of Minnesota Extension 405 Cofey Hall 1420 Eckles Avenue St. Paul, MN 55108-6068 Email to [email protected] or fax to 612-625-3967. University of Minnesota Extension shall provide equal access to and opportunity in its programs, facilites, and employment without regard to race, color, creed, religion, natonal origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientaton, gender identty, or gender expression. In accordance with the Americans with Disabilites Act, this publicaton/material is available in alternatve formats upon request. Direct requests to the Extension Regional Ofce, Cloquet at 218-726-6464. The informaton given in this publicaton is for educatonal purposes only. Reference to commercial products or trade names is made with the understanding that no discriminaton is intended and no endorsement by University of Minnesota Extension is implied. Acknowledgements Financial and other support for the Harvester Handbook came from University of Minnesota Extension, through the Extension Center for Food, Agricultural and Natural Resource Sciences (EFANS) and the Northeast Regional Sustainable Development Partnership (RSDP). Many individuals generously contributed to the development of the Handbook through original research, authorship of content, review of content, design and editng. Special thanks to Wendy Cocksedge and the Centre for Livelihoods and Ecology at Royal Roads University for their generosity with the Harvester Handbook concept. A special thanks to Trudy Fredericks for her tremen- dous overall eforts on this project. -
Ekim 2017 2.Cdr
Ekm(2017)8(2)125-128 Do :10.15318/Fungus.2 017.42 29.06.2017 Research Artcle 18.09.2017 An Interestng Half-Free Morel Record for Turkish Mycobiota (Morchella populiphila M. Kuo, M.C. Carter & J.D. Moore) İsmail ACAR1 , Yusuf UZUN2 1Department of Organic Agriculture, Başkale Vocational High School, Yüzüncü Yıl University, 65080, Van, Turkey 2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Yüzüncü Yıl University, 65080, Van, Turkey Abstract: Morchella species that are known as "Kuzu göbeği" in many regions of Turkey are among the mushrooms consumed all around the world. Moreover, Morels are a group of mushrooms attracting people's interest because of its high economic value. Our study material, Morchella populiphila M. Kuo, M.C. Carter & J.D. Moore was firstly reported in Van province in 2017. Short depiction and the photographs of the species are provided and discussed briefly. Key words: Mycobiota, Morchella populiphila, New record, Van Türkiye Mikobiyotası İçin İlgnç Bir Yarı-Serbest Morel Kaydı (Morchella populiphila M. Kuo, M.C. Carter & J.D. Moore) Öz: Türkiye'de birçok bölgede "Kuzu göbeği" mantarı olarak bilinen Morchella türleri tüm dünyada tüketilen mantarlar arasında yer alır. Moreller ekonomik değeri yüksek olmasından dolayı da insanların ilgisini çeken bir mantar grubudur. Çalışma materyalini oluşturan Morchella populiphila M. Kuo, M.C. Carter & J.D. Moore 2017 yılında yapılan arazi çalışmasında ilk kez Van ilinde rapor edilmiştir. Türün kısa betimi ve fotoğrafları verilmiş, kısaca tartışılmıştır. Anahtar kelimeler: Mikobiyota, Morchella populiphila, Yeni kayıt, Van Introduction previous studies, this species was reported in Morels are devided into two groups such as true Canada and the United States by Kuo et al. -
Checklist of the Species of the Genera Amanita and Limacella (Agaricomycetes) in Estonia
Folia Cryptog. Estonica, Fasc. 45: 81–85 (2009) Checklist of the species of the genera Amanita and Limacella (Agaricomycetes) in Estonia Mall Vaasma Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 181 Riia St., 51014, Tartu, Estonia. Natural History Museum, University of Tartu, 46 Vanemuise St., 51014, Tartu, Estonia. E-mail: [email protected] Abstract: 19 species, 2 varieties and 1 form of genus Amanita and 3 species of genus Limacella (Agaricomycetes) have been recorded in Estonia. A checklist of these species with habitat, phenology and occurrence data are presented. Kokkuvõte: Kärbseseene (Amanita) ja limalooriku (Limacella) perekonna (Agaricomycetes) liikide kriitiline nimestik Eestis Eestis on kärbseseene perekonnas 19 liiki, 2 teisendit ja 1 vorm, limalooriku perekonnas on 3 liiki. Igale liigile on antud andmed tema kasvukoha, fenoloogia ja esinemissageduse kohta. The present checklist contains 19 Amanita spe- FE – Neville, Poumarat, Fungi Europaei, 2004 cies, 2 varieties and 1 form and 3 Limacella spe- Galli – Galli, 2001 cies recorded in Estonia. All the species included GBW – Krieglsteiner, 2003 have been proved by relevant exsiccata in the KL – Kalamees & Liiv, 2005 mycological herbarium TAAM of the Institute of Korh – Korhonen, 2007 Agricultural and Environmental Sciences of the Lud – Ludwig, 2000 Estonian University of Life Sciences and in the Phil – Pillips, 2006 mycological herbarium TU of the Natural History RH – Ryman & Holmåsen, 2006 Museum of the University of Tartu. According to RM – Rivista di Micologia, 2008 literary sources (Urbonas a.o. 1986) Limacella SNS – Salo, Niemelä & Salo, 2006 delicata (Fr.) Earle has also been recorded in Estonia, but the exsiccata available do not en- AMANITA Pers., Tent. -
Classification of Amanita Species Based on Bilinear Networks With
agriculture Article Classification of Amanita Species Based on Bilinear Networks with Attention Mechanism Peng Wang, Jiang Liu, Lijia Xu, Peng Huang, Xiong Luo, Yan Hu and Zhiliang Kang * College of Mechanical and Electrical Engineering, Sichuan Agricultural University, Ya’an 625000, China; [email protected] (P.W.); [email protected] (J.L.); [email protected] (L.X.); [email protected] (P.H.); [email protected] (X.L.); [email protected] (Y.H.) * Correspondence: [email protected]; Tel.: +86-186-0835-1703 Abstract: The accurate classification of Amanita is helpful to its research on biological control and medical value, and it can also prevent mushroom poisoning incidents. In this paper, we constructed the Bilinear convolutional neural networks (B-CNN) with attention mechanism model based on transfer learning to realize the classification of Amanita. When the model is trained, the weight on ImageNet is used for pre-training, and the Adam optimizer is used to update network parameters. In the test process, images of Amanita at different growth stages were used to further test the generalization ability of the model. After comparing our model with other models, the results show that our model greatly reduces the number of parameters while achieving high accuracy (95.2%) and has good generalization ability. It is an efficient classification model, which provides a new option for mushroom classification in areas with limited computing resources. Keywords: deep learning; bilinear convolutional neural networks; attention mechanism; trans- fer learning Citation: Wang, P.; Liu, J.; Xu, L.; Huang, P.; Luo, X.; Hu, Y.; Kang, Z. -
Patterns of Ectomycorrhizal Host-Fungus Specificity in the Pacific Northwest
AN ABSTRACT OF THE THESIS OF Randolph John Molina for the degree of Doctor of Philosophy in Botany and Plant Pathology presented on December 17, 1980 Title: PATTERNS OF ECTOMYCORRHIZAL HOST-FUNGUS SPECIFICITY IN THE PACIFIC NORTHWEST Redacted for Privacy Abstract approved: Dr. James M. Trappe Results from approximately 400 fungus-host pure culture inoculations indicate that specificity of ectomycorrhizal associations is a complex phenomenon and cannot be based solely on field observations of sporocarp-host associations. Of the numerous sporocarp-host specific fungi tested, most formed ectomycorrhizae with one or more unexpected, non-associated hosts. These results conclusively demonstrate that ectomycorrhizal fungi which produce sporocarps only with a specific host species or genus can still form mycorrhizae with other "non-asso- ciated" hosts. The ability to form ectomycorrhizae with various hosts is termed "ectomycorrhizal host potential". Some fungi, however, showed superior mycorrhizal development on their particular hosts over other non-associated hosts, indicating further specialization in those associations. A large group of fungi known for diverse sporocarp-host associa- tions showed wide ectomycorrhizal host potential by forming abundant, well developed ectomycorrhizae with all or most hosts. It's suggested that these fungi may share similar compatibility or recognition factors common to many ectomycorrhizal hosts thus allowing for diverse host associations. A spectrum from mycorrhizal generalists to specialists was seen among the hosts in their ability to form mycorrhizae with diverse fungi. The ericaceous hosts Arctostaphylos uva-ursi and Arbutus menziesii were broadly receptive towards the fungi, forming mycorrhizae with 25 of the 28 tested. This included most of the fungi which produce sporocarps only in association with specific conifers. -
2 the Numbers Behind Mushroom Biodiversity
15 2 The Numbers Behind Mushroom Biodiversity Anabela Martins Polytechnic Institute of Bragança, School of Agriculture (IPB-ESA), Portugal 2.1 Origin and Diversity of Fungi Fungi are difficult to preserve and fossilize and due to the poor preservation of most fungal structures, it has been difficult to interpret the fossil record of fungi. Hyphae, the vegetative bodies of fungi, bear few distinctive morphological characteristicss, and organisms as diverse as cyanobacteria, eukaryotic algal groups, and oomycetes can easily be mistaken for them (Taylor & Taylor 1993). Fossils provide minimum ages for divergences and genetic lineages can be much older than even the oldest fossil representative found. According to Berbee and Taylor (2010), molecular clocks (conversion of molecular changes into geological time) calibrated by fossils are the only available tools to estimate timing of evolutionary events in fossil‐poor groups, such as fungi. The arbuscular mycorrhizal symbiotic fungi from the division Glomeromycota, gen- erally accepted as the phylogenetic sister clade to the Ascomycota and Basidiomycota, have left the most ancient fossils in the Rhynie Chert of Aberdeenshire in the north of Scotland (400 million years old). The Glomeromycota and several other fungi have been found associated with the preserved tissues of early vascular plants (Taylor et al. 2004a). Fossil spores from these shallow marine sediments from the Ordovician that closely resemble Glomeromycota spores and finely branched hyphae arbuscules within plant cells were clearly preserved in cells of stems of a 400 Ma primitive land plant, Aglaophyton, from Rhynie chert 455–460 Ma in age (Redecker et al. 2000; Remy et al. 1994) and from roots from the Triassic (250–199 Ma) (Berbee & Taylor 2010; Stubblefield et al.