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Health and Safety Handbook Department of Biological Sciences

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Health and Safety Handbook Department of Biological Sciences Health and Safety Handbook Department of Biological Sciences Xi’an Jiaotong-Liverpool University (XJTLU) 1 Latest update 6th September, 2019 (version v2.3) Preface This handbook, which supplements Standard Operating Procedures (SOPs), outlines the responsibilities and arrangements for ensuring your safety. It is provided to help you work safely and avoid accidents by providing a framework within which a safe method of work can be established. It is therefore important that you read the information given here at the start of your work in the Department of Biological Sciences. Accident prevention is mainly common sense, tidiness and specialist knowledge, but safety within laboratories does require constant vigilance and care. Remember that a little planning and thought can save a great deal of trouble and regret. Always seek expert advice when in doubt. You are required to sign and return the declaration issued with this handbook stating that you have read the handbook and are satisfied as to your, and the Department’s, responsibilities with respect to safety. This handbook will be reviewed at least annually and supplementary information distributed to all members of the Department. Suggestions for inclusion, corrections and revisions for future editions of this handbook should be sent to the Lab Manager (Ms Jie Jiang; Email: [email protected]; Tel.: 0512-8188 0482). 2 Latest update 6th September, 2019 (version v2.3) Table of Contents 1. Useful Information .................................................................................................................... 6 1.1. Emergency Telephone Number ........................................................................................ 6 1.2. First Aiders ......................................................................................................................... 6 1.3. Contact information for Health and Safety ..................................................................... 6 1.4. Other useful telephone numbers....................................................................................... 6 1.5. Introduction ........................................................................................................................ 7 1.6. Safety training .................................................................................................................... 7 1.7. General conducts and sanctions ....................................................................................... 7 1.8 Responsibilities .................................................................................................................... 9 1.8.1 Responsibilities for Department Biosafety Committee ............................................ 9 1.8.2 Responsibilities for Biosafety Officer ......................................................................... 9 1.8.3 Responsibilities for PIs ................................................................................................ 9 1.8.4 Responsibilities for lab users..................................................................................... 10 2. The paper work requirements ............................................................................................... 10 2.1. Standard Operating Procedure (SOP) ........................................................................... 11 2.2. Risk Assessment (RA) ...................................................................................................... 11 2.3. Control of Substances Hazardous to Health (CoSHH) ................................................ 12 2.4. Summary of controlling risk in the laboratory ............................................................. 13 3. General laboratories guidelines ............................................................................................. 13 3.1. Working in a laboratory .................................................................................................. 13 3.1.1. Set requirements for access to the laboratory ........................................................ 13 3.1.2. Set standards for appropriate behaviour in the laboratory.................................. 14 3.1.3 Enforce procedures to minimize the risk of sharps injuries. ................................. 15 3.1.4 Set procedures for routine decontamination, accidental spill cleanup, disposal of contaminated materials, and emergencies. ....................................................................... 15 3.2. Personal Protective Equipment (PPE) ........................................................................... 16 3.3. General laboratory tidiness ............................................................................................. 16 4. Chemicals and hazardous substances ................................................................................... 17 4.1. Labelling ........................................................................................................................... 17 4.2. Safe use, storage and disposal of chemicals ................................................................... 18 4.3. Handling chemicals .......................................................................................................... 18 4.4. Carcinogens ...................................................................................................................... 19 4.5. Disposal of chemicals ....................................................................................................... 19 4.6. Spillages ............................................................................................................................ 19 3 Latest update 6th September, 2019 (version v2.3) 5. Biological safety ....................................................................................................................... 20 5.1 General information ......................................................................................................... 20 5.2 Risk group classification................................................................................................... 21 5.3 Summary of Biological Safety Levels of Practices and Containment .......................... 22 5.4 Warning Signs and Postings ............................................................................................ 24 5.5 Safety Equipment .............................................................................................................. 25 5.6 Personal Protective Equipment ....................................................................................... 25 5.7 Waste management ........................................................................................................... 27 5.7.1 Housekeeping.............................................................................................................. 27 5.7.2 Sterilization and Disinfection .................................................................................... 27 5.7.3 Biological waste disposal ........................................................................................... 29 5.7.4 Biological Spill Clean-Up Procedures ...................................................................... 32 5.7.5 Emergency Procedures .............................................................................................. 32 6. General laboratory practices ................................................................................................. 33 6.1. Electrophoresis ................................................................................................................. 33 6.2. Chemicals .......................................................................................................................... 33 6.3. Ultraviolet (UV) radiation ............................................................................................... 34 6.4. Light microscopy .............................................................................................................. 34 6.5. Phenol ................................................................................................................................ 35 7.1 Physical containment features and zone classification .................................................. 35 7.2 Facility assignment procedures ....................................................................................... 37 7.3 Medical considerations ..................................................................................................... 38 7.5 Biohazard spill outside a biological safety cabinet (BSC) ............................................. 38 7.6 Biohazard spill inside a biological safety cabinet (BSC) ............................................... 40 7.7 Malfunction of biological safety cabinet ......................................................................... 41 7.8 Operational procedures .................................................................................................... 41 7.9 Waste disposal procedures ............................................................................................... 43 7.10 Facility operations: ......................................................................................................... 44 8. Electricity ................................................................................................................................. 45 9. Compressed gasses .................................................................................................................
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