^ ^ ^ ^ I ^ ^ ^ ^ II ^ II ^ ^ ^ ^ ^ II ^ ^ ^ ^ ^ ^ ^ ^ ^ I ^ � European Patent Office Office europeen des brevets EP 0 841 398 A3
(12) EUROPEAN PATENT APPLICATION
(88) Date of publication A3: (51) |ntmtci.6: C|.6: C12N 15/54, C12N 9/10, 06.10.1999 Bulletin 1999/40 C1C12N 2N 115/70, 5/70, C1C12N 2N 1/211/21, , C12P 19/18, A61K 31/70 (43),,,,n) Date of.... publication t. A2:AO ' 13.05.1998 Bulletin 1998/20
(21) Application number: 97308981.6
(22) Date of filing: 07.11.1997
(84) Designated Contracting States: • Kubota, Michio AT BE CH DE DK ES Fl FR GB GR IE IT LI LU MC Okayama-shi, Okayama (JP) NL PT SE • Chaen, Hiroto Okayama-shi, Okayama (JP) (30) Priority: 08.11.1996 JP 31123596 • Miyake, Toshio 03.03.1997 J P 6171097 Okayama-shi, Okayama (JP)
(71) Applicant: KABUSHIKI KAISHA HAYASHIBARA (74) Representative: Daniels, Jeffrey Nicholas et al SEIBUTSU KAGAKU KENKYUJO Page White & Farrer Okayama-shi Okayama (JP) 54 Doughty Street London WC1N2LS (GB) (72) Inventors: • Nishimoto, Tomoyuki Okayama-shi, Okayama (JP)
(54) Kojibiose Phosphorylase, its preparation and use
(57) A kojibiose phosphorylase which hydrolyzes transfer reaction of glucosyl group to other saccharides kojibiose in the presence of an inorganic phosphoric ac- using p-D-glucose-1 -phosphoric acid as a saccharide id to form D-glucose and p-D-glucose-1 -phosphoric ac- donor. The enzyme is obtainable from natural sources id, forms kojibiose and an inorganic phosphoric acid such as microorganisms of the genus Thermoanaerobi- from p-D-glucose-1 -phosphoric acid, and catalyzes the um, and obtainable by recombinant technology.
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Printed by Jouve, 75001 PARIS (FR) EP 0 841 398 A3
European Patent Number J EUROPEAN SEARCH REPORT Application Office EP 97 30 8981
DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document with indication, where appropriate, Relevant CLASSIFICATION OF THE of relevant passages to claim APPLICATION
DATABASE WPI 22-25 Derwent Publications Ltd., London, GB; AN 95-125550 XP002110120 & JP 07 046991 A (KIKKOMAN CORP), 21 February 1995 (1995-02-21) * abstract * 1,2,6,7, 12
DATABASE CHEMABS 'Online! 22-24,26 CHEMICAL ABSTRACTS SERVICE, COLUMBUS , OHIO, US FUJII, SAT0SHI ET AL: "Oligosaccharides TECHNICAL FIELDS SEARCHED produced by the transglucosidation action (lnl.CI.6) of Protaminobacter C12N rubrurti.alpha.-glucosidase" C12P retrieved from STN A61K Database accession no. 105:222353 XP002110118 ^ 3bstr*3ct *^ & SEIT0 GIJUTSU KENKYU KAISHI (1985), (34), 37-44 , GB 1 390 065 A (HAYASHIBARA CO) 22-26 9 April 1975 (1975-04-09) * claim 1 *
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The present search report has been drawn up for all claims Place of search Date of completion of the search Examiner THE HAGUE 10 August 1999 van de Kamp, M CATEGORY OF CITED DOCUMENTS T : theory or principle underlying the invention E : earlier patent document, but published on, or X : particularly relevant if taken alone after the filing date Y : particularly relevant if combined with another D : document cited in the application document of the same category L : document cited for other reasons A : technological background O : non-written disclosure & : member of the same patent family, corresponding P : intermediate document document
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European Patent EUROPEAN SEARCH REPORT Number Office EP 97 30 3981
DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document with indication, where appropriate, Relevant CLASSIFICATION OF THE of relevant passages to claim APPLICATION (lnt.CI.6) KITAO ET AL: "Formation of kojibiose and 22-24 nigerose by sucrose phosphorylase" BIOSCI. BIOTECH. BIOCHEM. , vol. 58, no. 4, 1994, pages 790-791, XP002111594 * abstract * 1,2,6,7, 12 * page 790, left-hand column, line 3 *
TAKEWAKI ET AL. : "Substrate specificity 1,2,6,7, and subsite affinities of honeybee alpha 12 glucosidase II" BIOSCI. BIOTECH. BIOCHEM., vol. 57, no. 9, 1993, pages 1508-1513, XP002111595 * abstract * * page 1508, right-hand column, line 9 * * table 1 *
AISAKA K ET AL: "Purification and 1,2,6,7, characterization of trehalose 12 TECHNICAL FIELDS phosphorylase from Catellatospora SEARCHED (lnt.CI.6) ferruginea. " BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, (1998 APR) 62 (4) 782-787, XP002110345 * page 785 - page 786 * * table 2 *
The present search report has been drawn up for all claims Placs of search Date of completion of the search Examiner THE HAGUE 10 August 1999 van de Kamp, M CATEGORY OF CITED DOCUMENTS T : theory or principle underlying the invention E : earlier patent document, but published on, or X : particularly relevant if taken alone after the filing date Y : particularly relevant if combined with another D : document cited in the application document of the same category L : document cited for other reasons A : technological background O : non-written disclosure & : member of the same patent family, corresponding P : intermediate document document
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ANNEX TO THE EUROPEAN SEARCH REPORT ON EUROPEAN PATENT APPLICATION NO. EP 97 30 8981
This annex lists the patent family members relating to the patent documents cited in the above-mentioned European search report. The members are as contained in the European Patent Office EDP file on The European Patent Office is in no way liable for these particulars which are merely given for the purpose of information. 10-08-1999
Patent document Publication Patent family Publication cited in search report date member(s) date JP 3022957 A 31-01-1991 JP 2832037 B 02-12-1998
JP 7046991 A 21-02-1995 NONE
GB 1390065 A 09-04-1975 JP 55012231 B 31-03-1980 BE 780129 A 04-09-1972 CH 556313 A 29-11-1974 DE 2210128 A 21-09-1972 FR 2128447 A 20-10-1972 IT 1048964 B 20-12-1980 NL 7202660 A 05-09-1972 ZA 7201358 A 27-12-1972
2i For more details about this annex : see Official Journal of the European Patent Office, No. 12/82
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