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Wo 2011/013133 A2 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date 3 February 2011 (03.02.2011) WO 2011/013133 A2 (51) International Patent Classification: Not classified DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, (21) International Application Number: KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, PCT/IN2009/000429 ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (22) International Filing Date: NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 28 July 2009 (28.07.2009) SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, (71) Applicant: THE ENERGY AND RESOURCES IN¬ GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, STITUTE (TERI) [IN/IN]; DS Block, IHC Complex, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, Lodhi Road, New Delhi 110 003 (IN). TM), European (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (72) Inventor: NUTAN, Kaushik; The Energy and Resources MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, Institute (TERI), DS Block, IHC Complex, Lodhi Road, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, New Delhi 110 003 (IN). ML, MR, NE, SN, TD, TG). (74) Common Representative: THE ENERGY AND RE¬ Declarations under Rule 4.17: SOURCES INSTITUTE (TERI); MOHAN, Joshi, — as to applicant's entitlement to apply for and be granted Madan, Director, Technology Dissemination and Enter a patent (Rule 4.1 7(H)) prise Development, DS Block, IHC Complex, Lodhi Road, New Delhi 110 003 (IN). Published: (81) Designated States (unless otherwise indicated, for every — without international search report and to be republished kind of national protection available): AE, AG, AL, AM, upon receipt of that report (Rule 48.2(g)) AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, (54) Title: NOVEL BIOPESTICIDE COMPOSITIONS AND METHOD FOR ISOLATION AND CHARACTERIZATION OF SAME (57) Abstract: The present invention describes the isolation and characterization of the novel biopesticide compositions and/or biopesticide formulations obtained from Eucalyptus species capable of serving as effective biocontrol agents and/or pest control management agents. The invention focuses on the isolation of these biopesticide compositions and formulations that are known to possess pesticidal properties and are derived from natural sources having biological origin. The invention more particularly de scribes the isolation and characterization, including but not confined to, novel biopesticide compositions possessing pesticidal at tributes along with other pharmaceutically important attributes so as to also function as effective biocontrol agents. Field of Invention This invention relates generally to the field of compositions and methods for controlling of pests and pest populations which are known to be having a detrimental effect on human life and human activities. The invention focuses on the isolation of these biopesticide compositions and formulations that are known to possess pesticidal properties and are derived from natural sources having biological origin. The invention more particularly describes the isolation and characterization, including but not confined to, novel biopesticide compositions possessing pesticidal attributes along with other pharmaceuticallyimportant attributes so as to also function as effective biocontrol agents. Background of Invention There is a large amount of activity in the general field of biopesticides derived from natural sources and having an essentially biological origin and their possible exploitation in the field of biocontrol. The prior art is flooded with a large number of patented inventions and technical literature on the subject in question. The genus Eucalyptus of family (Myrtaceae) owing to the presence of pharmacologically important oils and compounds as well as substances which have known pesticidal and biocontrol attributes has attracted interest of scientific community to investigate the nature, working mechanism and chemistry of these compounds. The genus Eucalyptus (Family Myrtaceae) shows a fairly large distribution in several regions of the world, with about 300 species being known. It is a native of Australian region, however the distribution today is fairly broad to several parts of Europe, South Africa, Northern Africa, America and even tropical countries like India. The Eucalyptus tree has its origin in Australia. Eucalyptus leaves and its oil have been traditionally used as Aboriginal cure for a wide range of diseases. Presently the £tvca/ypftvs*extracts are extensively used across the globe in pills, liquids, inhalers and ointments as a cure for several ailments that are general in nature. Field of Invention This invention relates generally to the field of compositions and methods for controlling of pests and pest populations which are known to be having a detrimental effect on human life and human activities. The invention focuses on the isolation of these biopesticide compositions and formulations that are known to possess pesticidal properties and are derived from natural sources having biological origin. The invention more particularly describes the isolation and characterization, including but not confined to, novel biopesticide compositions possessing pesticidal attributes along with other pharmaceutically important attributes so as to also function as effective biocontrol agents. Background of Invention There is a large amount of activity in the general field of biopesticides derived from natural sources and having an essentially biological origin and their possible exploitation in the field of biocontrol. The prior art is flooded with a large number of patented inventions and technical literature on the subject in question. The genus Eucalyptus of family (Myrtaceae) owing to the presence of pharmacologically important oils and compounds as well as substances which have known pesticidal and biocontrol attributes has attracted interest of scientific community to investigate the nature, working mechanism and chemistry of these compounds. The genus Eucalyptus (Family Myrtaceae) shows a fairly large distribution in several regions of the world, with about 300 species being known. It is a native of Australian region, however the distribution today is fairly broad to several parts of Europe, South Africa, Northern Africa, America and even tropical countries like India. The Eucalyptus tree has its origin in Australia. Eucalyptus leaves and its oil have been traditionally used as Aboriginal cure for a wide range of diseases. Presently the £uca/ypfus*extracts are extensively used across the globe in pills, liquids, inhalers and ointments as a cure for several ailments that are general in nature. It is a well known fact today that, plants are subject to attack by a great number of pests. These pests can be, for example, bacteria, fungi, or nematodes. Pesticidal compounds have long been used to increase yields and extend agricultural production capabilities into new areas. They have also been extremely important tools for ameliorating season-to-season differences in yield and quality caused by weather-driven variations in pest pressure. The aspect of the longstanding global demand for new, effective, environmentally friendly, and safe means to control pests that damage agriculture or serve as disease vectors is not new to the world today. As per one of the recent reports, in US alone the agriculture costs incurred by pests exceed billions of dollars annually in decreased crop yields, reduced crop quality, increased harvesting costs, pesticide application costs, and negative'ecological impact. In addition to agriculture pests, many blood-feeding insects and cockroaches are vectors for pathogenic microorganisms that threaten human and animal health, or are annoying at the least. As in the case of agriculture pests, direct and intangible costs incurred by blood-feeding and household pests concern pesticide safety hazards to humans and animals, bioaccumulation and environmental incompatibility, and synthesis and application costs. It is also common knowledge today that almost all field crops, nursery and horticulture plants, and commercial farming areas are susceptible to attack by one or more pests. The notable examples that cduld be cited are inclusive of the particularly problematic Coleopteran and Lepidopteran pests. A n example of a Lepidopteran pest is the hornworm larva of Manduca sexta, and an example of a Coleopteran pest is the Colorado potato beetle, Leptinotarsa decemlineata Vegetable and cole crops, lentils, leafy vegetables, melons, peppers, potatoes and related tubers, tomatoes, cucumbers and related vine crops, as well as a variety of spices are sensitive to infestation by one or more pests including loopers, armyworms, moth larvae, bud webworms, earworms, leafeaters, «worms, borers, cloverworms, melonworms, leafrollers, various caterpillars, fruitworms, homworms, and pinworms. Likewise, pasture and hay crops such as alfalfa, pasture and forage grasses and silage are often attacked by a variety of pests including armyworms, alfalfa caterpillars, European skipper, a variety of loopers
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