bioRxiv preprint doi: https://doi.org/10.1101/2020.06.30.179812; this version posted June 30, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 A global analysis of song frequency in passerines provides no support for the acoustic 2 adaptation hypothesis but suggests a role for sexual selection 3 Running title: Evolutionary ecology of song frequency 4 5 Peter Mikula1,2, Mihai Valcu3, Henrik Brumm4, Martin Bulla3,5, Wolfgang Forstmeier3, 6 Tereza Petrusková6, Bart Kempenaers3*, Tomáš Albrecht1,2* 7 8 1Department of Zoology, Faculty of Science, Charles University, Viničná 7, 128 44, Praha 2, 9 Czech Republic 10 2Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, 603 65, Brno, Czech 11 Republic 12 3Department of Behavioural Ecology and Evolutionary Genetics, Max Planck Institute for 13 Ornithology, Eberhard-Gwinner-Str. 7, 82319 Seewiesen, Germany 14 4Communication and Social Behaviour Group, Max Planck Institute for Ornithology, 15 Eberhard-Gwinner-Str. 11, 82319 Seewiesen, Germany 16 5Department of Ecology, Faculty of Environmental Sciences, Czech University of Life 17 Sciences, 16521 Prague, Czech Republic 18 6Department of Ecology, Faculty of Science, Charles University, Viničná 7, 128 43, Praha 2, 19 Czech Republic 20 *Authors for correspondence: E-mail:
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[email protected], 21 phone no.: +498 157 932 232 and +420 608 237 158 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.06.30.179812; this version posted June 30, 2020.