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Austral Journal of Veterinary Sciences REVIEW ARTICLE The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction. Electrochemotherapy in the treatment of neoplasms in dogs and cats. Luis D. Ortega-Morales, Alicia Alcantar-Rodriguez, Maria C. Espejel, Alfredo Marcelo M.M. Rangel, Jean C.S. Luz, Krishna D. Oliveira, Javier Ojeda, Jennifer O. Medrano Freytag, Daniela O. Suzuki Faecal shedding of campylobacteria among domestic and wild animals from an urban coastal area. ORIGINAL ARTICLES Arturo Levican, Oriana Flores, Sergio Sanchez, María G. Bascuñan, Rafael Lopez, Karla Ojeda, Eduardo Hernandez, Pablo Salah Evaluation of reduced amino acids diets added with protected protease on productive performance in 25-100 kg barrows. Burnout syndrome prevalence in veterinarians working in Chile. Jose L. Figueroa, Jose A. Martinez, Maria T. Sanchez-Torres, Jose L. Cordero, Romy M. Weinborn, Braulio J. Bruna, Joan Calventus, Gerardo A. Sepúlveda Manuel Martinez Victor M. Valdez, Agustin Ruiz Comparison of yield characteristics of Damascus and Kilis goats in dry climatic SHORT COMMUNICATIONS conditions. Ali Murat Tatar, Selçuk Seçkin Tuncer, Halit Deniz Şireli Hemicastration in Chilean rodeo stallions in competition. Lisandro Muñoz, Consuelo Blu, Reinaldo Ortiz, Jaime Cruces, María Cordero, The effect of ginger on testis of Broiler breeders. Mario Briones Mansour Hamzehnezhad, Hossein Erik-Aghaji, Afshin Zakeri, Sajjad Hejazi Austral Journal of Veterinary Sciences Austral Journal of Veterinary Sciences ISSN 0719-8000 / ISSN 0719-8132 VOL. 51 - Nº 2 2019 VOL. VOLUME 51 / VALDIVIA - CHILE / 2019 / Nº 2 VOLUME 51 / VALDIVIA - CHILE / 2019 / Nº 2 phd programme diploma PHD PROGRAMME IN VETERINARY DIPLOMA IN FOOD SAFETY SCIENCES Coordinator: Carmen López Programme accredited by the National This journal is subsidised by Accreditation Commission (CNA) DIPLOMA IN QUALITY OF MEAT Dirección de Investigación y Desarrollo de la Universidad Austral de Chile (DID-UACh) Coordinator: Prof. Paulo Corti PRODUCTS Coordinator: Carmen Gallo Periodicity: Triannual (January-May-September). Funded in 1969. DIPLOMA IN APPLIED RESEARCH Indexed in: TECHNIQUES FOR WILDLIFE – Current Contents Agriculture, Biology and Environmental Sciences (CC/AB and ES). MANAGEMENT – Commonwealth Agricultural Bureau, International (C.A.B.I.). Coordinator: Angelo Espinoza – Dairy Science Abstracts. – Veterinary Bulletin. – Animal Breeding Abstracts. clinical graduate master of science – Helminthological Abstracts S.A. – Agrindex. – Biological Abstracts. POSTGRADUATE IN VETERINARY MASTER OF SCIENCE IN ANIMAL – Periódica. CLINICAL SCIENCES HEALTH – Focus on: Veterinary Sciences and Medicine. Coordinator: Assistant Prof. Marcelo Mieres Programme accredited by the National – Science Citation Index Expanded. Accreditation Commission (CNA) – Scielo - Chile. POSTGRADUATE IN RUMINANT (2014-2020) – Google Scholar. – Ebsco. HUSBANDRY Coordinator: Assistant Prof. Javier Ojeda Coordinator: Claudia Letelier MASTER OF SCIENCE IN ANIMAL SCIENCE Coordinator: Assistant Prof. Nancy Jerez MASTER IN PREVENTIVE VETERINARY MEDICINE Coordinator: Assistant Prof. Cristóbal Verdugo INFORMATION AND APPLICATIONS Escuela de Graduados – Facultad de Ciencias Veterinarias – Universidad Austral de Chile Tel. 56-63-2221548 – Casilla 567 – Valdivia – Chile e-mail: [email protected] – www.uach.cl ISSN 0719-8000 ISSN 0719-8132 Austral Journal of Veterinary Sciences VOL. 51, Nº 2, 2019 Editorial Committee President: Javier Ojeda O., DMV, M.Sc. Luis Vargas Ch., M.B., Ph.D. Miguel Salgado A., DMV, Mg.Sc., Ph.D. Claudio Henríquez Sch., DMV, M.Sc., Ph.D. Editorial Assistant: Claudia Cárdenas A., Agronomist Editorial Advisory Committee Jon Arnemo, DMV, Ph.D. - Hedmark University College, Norway Carmen Fuentealba, DMV, M.Sc., Ph.D. - Ross University, St. Kitts & Nevis Rodrigo Gallardo, DMV, Ph.D. - University of California Davis, USA Carlos Hermosilla, DMV, Ph.D., DipEVPC, Dr. habil. - Justus Liebig University, Germany Faham Khamesipour, DVM, M.Sc., Ph.D. - Shiraz University, Iran Giovanna Liguori, DMV, Ph.D. - Università degli Studi di Napoli “Federico II”, Italy Raúl Mainar, DMV, M.Sc., Ph.D., DipECVPH - Centro de Invest. y Tec. Agroalimentaria, España José Luis Muñoz, M.B., Ph.D. - Universidad de Los Lagos, Chile Alessandra Pelagalli, B.Pharm., Ph.D. - Università degli Studi di Napoli “Federico II”, Italy Manuel Quezada, DMV, Ph.D. - Universidad de Concepción, Chile Sergio Recabarren, B.S. - Universidad de Concepción, Chile Pedro Smith, DMV, M.Sc.- Universidad de Chile, Chile Jorge Toro, DMV, M.Sc., Ph.D. - Universidad Austral de Chile, Chile Francisco Uzal, DMV, M.Sc., Ph.D., Dipl. ACVP - University of California Davis, USA Gerdien van Schaik, M.Sc., Dipl Anim Sci, Ph.D. - Gezondheidsdienst voor Dieren, The Netherlands Noel Verjan, DMV, M.Sc., Ph.D. - Universidad de Tolima, Colombia Universidad Austral de Chile Facultad de Ciencias Veterinarias Casilla 567 - Valdivia - Chile Cover: Micrograph showing fluorescent merocyanine patterns on dog spermatozoa: opaque - low fluidity (spermatozoon on the left) or brilliant - high fluidity (spermatozoa on the right). Photograph captured by Alicia Alcantar-Rodríguez and provided by Ortega-Morales et al. This journal is licensed under Creative Commons 4.0 (CC BY-NC-ND 4.0) VOLUME 51, Nº 2, 2019 EDITORIAL V REVIEW ARTICLE Electrochemotherapy in the treatment of neoplasms in dogs and cats. Marcelo M.M. Rangel, Jean C.S. Luz, Krishna D. Oliveira, Javier Ojeda, Jennifer O. Freytag, Daniela O. Suzuki 45 ORIGINAL ARTICLES Evaluation of reduced amino acids diets added with protected protease on productive performance in 25-100 kg barrows. Jose L. Figueroa, Jose A. Martinez, Maria T. Sanchez-Torres, Jose L. Cordero, Manuel Martinez Victor M. Valdez, Agustin Ruiz 53 Comparison of yield characteristics of Damascus and Kilis goats in dry climatic conditions. Ali Murat Tatar, Selçuk Seçkin Tuncer, Halit Deniz Şireli 61 The effect of ginger on testis of Broiler breeders. Mansour Hamzehnezhad, Hossein Erik-Aghaji, Afshin Zakeri, Sajjad Hejazi 67 The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction. Luis D. Ortega-Morales, Alicia Alcantar-Rodriguez, Maria C. Espejel, Alfredo Medrano 73 Faecal shedding of campylobacteria among domestic and wild animals from an urban coastal area. Arturo Levican, Oriana Flores, Sergio Sanchez, María G. Bascuñan, Rafael Lopez, Karla Ojeda, Eduardo Hernandez, Pablo Salah 83 Burnout syndrome prevalence in veterinarians working in Chile. Romy M. Weinborn, Braulio J. Bruna, Joan Calventus, Gerardo A. Sepúlveda 91 SHORT COMMUNICATIONS Hemicastration in Chilean rodeo stallions in competition. Lisandro Muñoz, Consuelo Blu, Reinaldo Ortiz, Jaime Cruces, María Cordero, Mario Briones 101 Austral J Vet Sci 51, V (2019) Editorial The magic number Since the novel of Douglas Adams, The Hitchhiker’s Guide to the Galaxy (1979), where the answer of a supercomputer to “the most important question”, including life and the universe, is the number 42, no one has been so attached to a single number as scientists. This is the purpose that we often assigned to P-values, an arbitrary threshold delimiting “statistical significance”. Recently, more than 800 scientists from 50 countries signed a letter requesting the withdrawal of all concepts of Statistical Significance1. The dichotomized discussion involves the terms statistically “significant” and “non-significant”, which depend on whether the P-value falls below or above this “magic” number, giving the idea that the items evaluated are categorically different. Unfortunately, the false belief that crossing the threshold of statistical significance is enough to show that a result is ‘real’ has led scientists and us (journal editors) to privilege such results, thereby distorting the literature. Some could argue that an arbitrary but clear limit is better than an unclear one. Additionally, if we do not use statistical significance, then what should we be doing instead? Whatever the discussion might lead to in the future, what seems relevant now is to educate ourselves about statistical misconceptions, but further, we should consider the logic, the background knowledge, and the experimental design along with P value to conclude. Austral Journal of Veterinary Sciences is not considering changing how it considers the statistical analysis in evaluation of papers at this time, but we are aware of this discussion in the scientific world since the conclusions will necessarily have an impact on what is currently considered suitable for publication. Editorial Committee Austral Journal of Veterinary Sciences 1 https://www.nature.com/articles/d41586-019-00857-9 Austral J Vet Sci 51, 45-51 (2019) REVIEW Electrochemotherapy in the treatment of neoplasms in dogs and cats Marcelo M.M. Rangela*, Jean C.S. Luza, Krishna D. Oliveiraa, Javier Ojedab, Jennifer O. Freytaga, Daniela O. Suzukic ABSTRACT. Electrochemotherapy (ECT) is a technique that combines chemotherapy with local application of specific electric pulses with the aim of increasing the permeability of the plasma membrane in a reversible way, improving the influx of chemotherapeutic drugs into the cytoplasm and potentiating their cytotoxic effects. This technique has broadened the range of possible treatments for oncological patients, either on its own or as adjuvant to surgical procedures. It is especially useful in tumors located in regions with only a small surgical safety margin, such as the limb extremities, skull, oral cavity, neck
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