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Antioxidants and Second Messengers of Free Radicals
antioxidants Antioxidants and Second Messengers of Free Radicals Edited by Neven Zarkovic Printed Edition of the Special Issue Published in Antioxidants www.mdpi.com/journal/antioxidants Antioxidants and Second Messengers of Free Radicals Antioxidants and Second Messengers of Free Radicals Special Issue Editor Neven Zarkovic MDPI • Basel • Beijing • Wuhan • Barcelona • Belgrade Special Issue Editor Neven Zarkovic Rudjer Boskovic Institute Croatia Editorial Office MDPI St. Alban-Anlage 66 4052 Basel, Switzerland This is a reprint of articles from the Special Issue published online in the open access journal Antioxidants (ISSN 2076-3921) from 2018 (available at: https://www.mdpi.com/journal/ antioxidants/special issues/second messengers free radicals) For citation purposes, cite each article independently as indicated on the article page online and as indicated below: LastName, A.A.; LastName, B.B.; LastName, C.C. Article Title. Journal Name Year, Article Number, Page Range. ISBN 978-3-03897-533-5 (Pbk) ISBN 978-3-03897-534-2 (PDF) c 2019 by the authors. Articles in this book are Open Access and distributed under the Creative Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons license CC BY-NC-ND. Contents About the Special Issue Editor ...................................... vii Preface to ”Antioxidants and Second Messengers of Free Radicals” ................ ix Neven Zarkovic Antioxidants and Second Messengers of Free Radicals Reprinted from: Antioxidants 2018, 7, 158, doi:10.3390/antiox7110158 ............... -
Oecd Guideline for the Testing of Chemicals
474 Adopted: 21st July 1997 OECD GUIDELINE FOR THE TESTING OF CHEMICALS Mammalian Erythrocyte Micronucleus Test INTRODUCTION 1. The mammalian in vivo micronucleus test is used for the detection of damage induced by the test substance to the chromosomes or the mitotic apparatus of erythroblasts by analysis of erythrocytes as sampled in bone marrow and/or peripheral blood cells of animals, usually rodents. 2. The purpose of the micronucleus test is to identify substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments or whole chromosomes. 3. When a bone marrow erythroblast develops into a polychromatic erythrocyte, the main nucleus is extruded; any micronucleus that has been formed may remain behind in the otherwise anucleated cytoplasm. Visualisation of micronuclei is facilitated in these cells because they lack a main nucleus. An increase in the frequency of micronucleated polychromatic erythrocytes in treated animals is an indication of induced chromosome damage. 4. Definitions used are set out in the Annex. INITIAL CONSIDERATIONS 5. The bone marrow of rodents is routinely used in this test since polychromatic erythrocytes are produced in that tissue. The measurement of micronucleated immature (polychromatic) erythrocytes in peripheral blood is equally acceptable in any species in which the inability of the spleen to remove micronucleated erythrocytes has been demonstrated, or which has shown an adequate sensitivity to detect agents that cause structural or numerical chromosome aberrations. Micronuclei can be distinguished by a number of criteria. These include identification of the presence or absence of a kinetochore or centromeric DNA in the micronuclei. The frequency of micronucleated immature (polychromatic) erythrocytes is the principal endpoint. -
Anuran Assemblage Changes Along Small-Scale Phytophysiognomies in Natural Brazilian Grasslands
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.31.229310; this version posted August 3, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Anuran assemblage changes along small-scale phytophysiognomies in natural Brazilian grasslands Diego Anderson Dalmolin1*, Volnei Mathies Filho2, Alexandro Marques Tozetti3 1 Laboratório de Metacomunidades, Departamento de Ecologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. 2 Fundação Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brasil 3Laboratório de Ecologia de Vertebrados Terrestres, Universidade do Vale do Rio dos Sinos, Avenida Unisinos 950, 93022-000 São Leopoldo, Rio Grande do Sul, Brazil. * Corresponding author: Email: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/2020.07.31.229310; this version posted August 3, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Abstract 2 We studied the species composition of frogs in two phytophysiognomies (grassland and 3 forest) of a Ramsar site in southern Brazil. We aimed to assess the distribution of 4 species on a small spatial scale and dissimilarities in community composition between 5 grassland and forest habitats. The sampling of individuals was carried out through 6 pitfall traps and active search in the areas around the traps. -
S2(R1) Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use
Guidance for Industry S2(R1) Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Biologics Evaluation and Research (CBER) June 2012 ICH Guidance for Industry S2(R1) Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use Additional copies are available from: Office of Communications Division of Drug Information, WO51, Room 2201 Center for Drug Evaluation and Research Food and Drug Administration 10903 New Hampshire Ave., Silver Spring, MD 20993-0002 Phone: 301-796-3400; Fax: 301-847-8714 [email protected] http://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/default.htm and/or Office of Communication, Outreach and Development, HFM-40 Center for Biologics Evaluation and Research Food and Drug Administration 1401 Rockville Pike, Rockville, MD 20852-1448 http://www.fda.gov/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/default.htm (Tel) 800-835-4709 or 301-827-1800 U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Biologics Evaluation and Research (CBER) June 2012 ICH Contains Nonbinding Recommendations TABLE OF CONTENTS I. INTRODUCTION (1)....................................................................................................... 1 A. Objectives of the Guidance (1.1)...................................................................................................1 -
The Optimized Conditions for the in Vitro Micronucleus (MN) Test Procedures Using Chamber Slides
Environ. Mutagen Res., 27: 145-151 (2005) Original Article The optimized conditions for the in vitro micronucleus (MN) test procedures using chamber slides Mika Yamamoto*, Akira Motegi, Jiro Seki and Youichi Miyamae Toxicology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd. 1-6, Kashima 2-chome, Yodogawa-ku, Osaka 532-8514, Japan Summary Optimized conditions for an in vitro micronucleus (MN) test procedure were defined using a chamber slide that enabled the preparation of fine specimens without undergoing complicating pro- cedures using culture dishes. The issues investigated are 1) the effect of slide materials on the adhesion of cells, 2) the number of seeding cells necessary to obtain an adequate number of cells for observation and 3) effects of hypotonic treatment and fixation on the cytoplasmic: nuclear area ratio. In addition, we determined cell viability in each chamber using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay. The results of the investigation were as follows: 1) cell adhesion was best using plastic slides, 2) the optimum number of cells for seeding was 6.6× 103 cells/cm2, 3) the best condition for hypotonic treatment was incubation in 75 mM KCl at 37 ℃ for 5 min, and 4) the best condition for fixation was treatment of cells twice for about 2 min in ice- cold methanol containing 6% acetic acid. Finally, the result of the MTT assay correlated with the number of viable cells in chamber as determined by the trypan blue dye exclusion assay. An in vitro MN test was conducted under these conditions using the known clastogens, Mitomycin C and dimethylnitrosamine. -
Development of a Novel Micronucleus Assay in the Human 3-D Skin Model, Epidermtm
Development of a Novel Micronucleus Assay in the Human 3-D Skin Model, EpiDermTM. R D Curren1, G Mun1, D P Gibson2, and M J Aardema2. 1Institute for In VitroSciences, Inc., Gaithersburg, MD; 2The Procter & Gamble Co., Cincinnati, OH. Presented at the 44nd Annual Meeting of the Society of Toxicology New Orleans, Louisiana March 6-10, 2005 Abstract The rodent in vivo micronucleus assay is an important part of a tiered testing strategy in genetic toxicology. However, this assay, in general, only provides information about materials available systemically, not at the point of contact, e.g. skin. Although in vivo rodent skin micronucleus assays are being developed, the results will still require extrapolation to the human. Furthermore, to fully comply with recent European legislation such as the 7th Amendment to the Cosmetics Directive, non-animal test methods will be needed to assess new chemicals and ingredients. Therefore we have begun development of a micronucleus assay using a commercially available 3-D engineered skin model of human origin, EpiDermTM (MatTek Corp, Ashland, MA). We first evaluated whether a population of binucleated cells sufficient for a micronucleus assay could be obtained by exposing the tissue to 1-3 ug/ml cytochalasin B (Cyt B). The frequency of binucleated cells increased both with time (to at least 120 h) and with increasing concentration of Cyt B. Three ug/ml Cyt B allowed us to reliably obtain 40-50% binucleated cells at 48h. Mitomycin C (MMC) was then used (in the presence of 3 ug/ml Cyt B) to investigate toxicity and micronuclei formation in EpiDermTM. -
In Vivo Micronucleus Assay. Chronic Toxicity Study, Pursuant to the (A) Purpose
Environmental Protection Agency § 79.64 Program 13-week Studies.’’ Fundam. period and femoral bone marrow is ex- Appl. Toxicol. 11:343–358. tracted. The bone marrow is then (7) Russell, L.D., Ettlin, R.A., smeared onto glass slides, stained, and Sinhattikim, A.P., and Clegg, E.D PCEs are scored for micronuclei. Re- (1990) Histological and searchers may need to run a trial at Histopathological Evaluation of the the highest tolerated concentration of Testes, Cache River Press, Clearwater, the test atmosphere to optimize the FL. sample collection time for [59 FR 33093, June 27, 1994, as amended at 61 micronucleated cells. FR 36513, July 11, 1996] (ii) This assay may be done sepa- rately or in combination with the sub- § 79.64 In vivo micronucleus assay. chronic toxicity study, pursuant to the (a) Purpose. The micronucleus assay provisions in § 79.62. is an in vivo cytogenetic test which (2) Species and strain. (i) The rat is uses erythrocytes in the bone marrow the recommended test animal. Other of rodents to detect chemical damage rodent species may be used in this to the chromosomes or mitotic appa- assay, but use of that species will be ratus of mammalian cells. As the justified by the tester. erythroblast develops into an eryth- (ii) If a strain of mouse is used in this rocyte (red blood cell), its main nu- assay, the tester shall sample periph- cleus is extruded and may leave a eral blood from an appropriate site on micronucleus in the cell body; a few the test animal, e.g., the tail vein, as a micronuclei form under normal condi- source of normochromatic tions in blood elements. -
AMPHIBIA: ANURA: LEPTODACTYLIDAE Leptodactylus Pentadactylus
887.1 AMPHIBIA: ANURA: LEPTODACTYLIDAE Leptodactylus pentadactylus Catalogue of American Amphibians and Reptiles. Heyer, M.M., W.R. Heyer, and R.O. de Sá. 2011. Leptodactylus pentadactylus . Leptodactylus pentadactylus (Laurenti) Smoky Jungle Frog Rana pentadactyla Laurenti 1768:32. Type-locality, “Indiis,” corrected to Suriname by Müller (1927: 276). Neotype, Nationaal Natuurhistorisch Mu- seum (RMNH) 29559, adult male, collector and date of collection unknown (examined by WRH). Rana gigas Spix 1824:25. Type-locality, “in locis palu - FIGURE 1. Leptodactylus pentadactylus , Brazil, Pará, Cacho- dosis fluminis Amazonum [Brazil]”. Holotype, Zoo- eira Juruá. Photograph courtesy of Laurie J. Vitt. logisches Sammlung des Bayerischen Staates (ZSM) 89/1921, now destroyed (Hoogmoed and Gruber 1983). See Nomenclatural History . Pre- lacustribus fluvii Amazonum [Brazil]”. Holotype, occupied by Rana gigas Wallbaum 1784 (= Rhin- ZSM 2502/0, now destroyed (Hoogmoed and ella marina {Linnaeus 1758}). Gruber 1983). Rana coriacea Spix 1824:29. Type-locality: “aquis Rana pachypus bilineata Mayer 1835:24. Type-local MAP . Distribution of Leptodactylus pentadactylus . The locality of the neotype is indicated by an open circle. A dot may rep - resent more than one site. Predicted distribution (dark-shaded) is modified from a BIOCLIM analysis. Published locality data used to generate the map should be considered as secondary sources, as we did not confirm identifications for all specimen localities. The locality coordinate data and sources are available on a spread sheet at http://learning.richmond.edu/ Leptodactylus. 887.2 FIGURE 2. Tadpole of Leptodactylus pentadactylus , USNM 576263, Brazil, Amazonas, Reserva Ducke. Scale bar = 5 mm. Type -locality, “Roque, Peru [06 o24’S, 76 o48’W].” Lectotype, Naturhistoriska Riksmuseet (NHMG) 497, age, sex, collector and date of collection un- known (not examined by authors). -
Baixar Este Arquivo
1 Https://online.unisc.br/seer/index.php/cadpesquisa ISSN on-line: 1677-5600 Doi: 10.17058/cp.v29i1.11152 Universidade de Santa cruz do Sul - Unisc Recebido em 06 de Fevereiro de 2017 Aceito em 17 de Maio de 2017 Autor para contato: [email protected] Fungos aquáticos (Oomycota, Chytridiomycota) ocorrentes em anfíbios anuros em dois remanescentes de Mata Mtlântica, localizados em Santa Cruz do Sul e Venâncio Aires, RS, Brasil Aquatic fungi (Oomycota, Chytridiomycota) ocurring in anuran amphibians in two remaining of the Atlantic Forest in Santa Cruz do Sul and Venâncio Aires municipalilties, Southern Brazil Francine Kist Closs Universidade de Santa Cruz do Sul – Unisc – Santa Cruz do Sul – Rio Grande do Sul - Brasil Jair Putzke Universidade Federal do Pampa – Unipampa – Bagé – Rio Grande do Sul – Brasil Resumo Palavras-chave Fungos patógenos de anfíbios estão entre os maiores vilões à biodiversidade de anuros, sendo de Fungos Zoospóricos. extrema importância um maior conhecimento destes organismos causadores de doenças. Buscou-se Patógenos. Mata Atlântica. conhecer os fungos zoospóricos ocorrentes em anuros em remanescentes de Mata Atlântica na Anurofauna. região de Venâncio Aires e Santa Cruz do Sul – RS. As amostragens em campo realizaram-se entre outubro de 2014 e abril de 2015, em Linha Estrela em Venâncio Aires e dentro da UNISC em Santa Cruz do Sul. As amostragens de anuros foram feitas a partir do método de procura visual ativa e procura em sítios de reprodução. Em laboratório, foi utilizado o método de isolamento por iscas para fungos aquáticos, adaptada. Foram amostrados 24 indivíduos de Anura, compostos por 9 espécies, enquadrados em 3 famílias (Hylidae, Leptodactylidae e Leiuperidae). -
Review Article SOME PLANTS AS a SOURCE of ACETYL CHOLINESTERASE INHIBITORS: a REVIEW Purabi Deka *, Arun Kumar, Bipin Kumar Nayak, N
Purabi Deka et al. Int. Res. J. Pharm. 2017, 8 (5) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 – 8407 Review Article SOME PLANTS AS A SOURCE OF ACETYL CHOLINESTERASE INHIBITORS: A REVIEW Purabi Deka *, Arun Kumar, Bipin Kumar Nayak, N. Eloziia Division of Pharmaceutical Sciences, Shri Guru Ram Rai Institute of Technology & Science, Dehradun, India *Corresponding Author Email: [email protected] Article Received on: 27/03/17 Approved for publication: 27/04/17 DOI: 10.7897/2230-8407.08565 ABSTRACT The term dementia derives from the Latin demens (“de” means private, “mens” means mind, intelligence and judgment- “without a mind”). Dementia is a progressive, chronic neurological disorder which destroys brain cells and causes difficulties with memory, behaviour, thinking, calculation, comprehension, language and it is brutal enough to affect work, lifelong hobbies, and social life. Alzheimer’s disease, Parkinson’s disease, Dementia with Lewys Bodies are some common types of dementias. Acetylcholinesterase AChE) Inhibition, the key enzyme which plays a main role in the breakdown of acetylcholine and it is considered as a Positive strategy for the treatment of neurological disorders. Currently many AChE inhibitors namely tacrine, donepezil, rivastigmine, galantamine have been used as first line drug for the treatment of Alzheimer’s disease. They are having several side effects such as gastrointestinal disorder, hepatotoxicity etc, so there is great interest in finding new and better AChE inhibitors from Natural products. Natural products are the remarkable source of Synthetic as well as traditional products. Abundance of plants in nature gives a potential source of AChE inhibitors. The purpose of this article to present a complete literature survey of plants that have been tested for AChE inhibitory activity. -
Amphibia: Anura)
MUSEU PARAENSE EMÍLIO GOELDI UNIVERSIDADE FEDERAL DO PARÁ PROGRAMA DE PÓS-GRADUAÇÃO EM ZOOLOGIA CURSO DE DOUTORADO EM ZOOLOGIA ESTUDOS CROMOSSÔMICOS EM ANUROS DAS FAMÍLIAS HYLIDAE RAFINESQUE, 1815 E LEPTODACTYLIDAE WERNER, 1896 (AMPHIBIA: ANURA) PABLO SUÁREZ Tese apresentada ao Programa de Pós-graduação em Zoologia, Curso de Doutorado, do Museu Paraense Emílio Goeldi e Universidade Federal do Pará como requisito para obtenção do grau de doutor em Zoologia. Orientador: Dr. Julio César Pieczarka BELÉM – PARÁ 2010 Livros Grátis http://www.livrosgratis.com.br Milhares de livros grátis para download. II PABLO SUÁREZ ESTUDOS CROMOSSÔMICOS EM ANUROS DAS FAMÍLIAS HYLIDAE RAFINESQUE, 1815 E LEPTODACTYLIDAE WERNER, 1896 (AMPHIBIA: ANURA) Tese apresentada ao Programa de Pós-graduação em Zoologia, Curso de Doutorado, do Museu Paraense Emílio Goeldi e Universidade Federal do Pará como requisito para obtenção do grau de doutor em Zoologia Orientador: Dr. Julio César Pieczarka BELÉM – PARÁ 2010 III PABLO SUÁREZ ESTUDOS CROMOSSÔMICOS EM ANUROS DAS FAMÍLIAS HYLIDAE RAFINESQUE, 1815 E LEPTODACTYLIDAE WERNER, 1896 (AMPHIBIA: ANURA) Banca examinadora Dr. Julio César Pieczarka (Orientador) ICB (Belém) – UFPa Membros Dra. Luciana Bolsoni Lourenço IB/UNICAMP Dr. Odair Aguiar Junior Biociências/UNIFESP Dr. Evonnildo Costa Gonçalves ICB/UFPA Dr. Marinus S. Hoogmoed CZO/MPEG IV DEDICATÓRIA a minha família V AGRADECIMENTOS - Ao Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Museu Paraense Emilio Goeldi (MPEG), Universidade Federal do Pará (UFPa) e à Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) pelo financiamento do Projeto de Pesquisa; - Ao Instituto Brasileiro de Meio Ambiente (IBAMA) por conceder as licenças para a coleta dos animais estudados; - Ao Laboratório de Citogenética Animal pelo fornecimento de toda a infraestrutura acadêmico-científica, sem as quais o trabalho não se realizaria; - À coordenadoria do Curso de Pós-Graduação em Zoologia do Museu Paraense Emilio Goeldi pelo encaminhamento das questões burocrático-acadêmicas; - Ao Dr. -
Leptodactylus Bufonius Sally Positioned. the Oral Disc Is Ventrally
905.1 AMPHIBIA: ANURA: LEPTODACTYLIDAE Leptodactylus bufonius Catalogue of American Amphibians and Reptiles. Schalk, C. M. and D. J. Leavitt. 2017. Leptodactylus bufonius. Leptodactylus bufonius Boulenger Oven Frog Leptodactylus bufonius Boulenger 1894a: 348. Type locality, “Asunción, Paraguay.” Lectotype, designated by Heyer (1978), Museum of Natural History (BMNH) Figure 1. Calling male Leptodactylus bufonius 1947.2.17.72, an adult female collected in Cordillera, Santa Cruz, Bolivia. Photograph by by G.A. Boulenger (not examined by au- Christopher M. Schalk. thors). See Remarks. Leptodactylus bufonis Vogel, 1963: 100. Lap- sus. sally positioned. Te oral disc is ventrally po- CONTENT. No subspecies are recognized. sitioned. Te tooth row formula is 2(2)/3(1). Te oral disc is slightly emarginated, sur- DESCRIPTION. Leptodactylus bufonius rounded with marginal papillae, and possess- is a moderately-sized species of the genus es a dorsal gap. A row of submarginal papil- (following criteria established by Heyer and lae is present. Te spiracle is sinistral and the Tompson [2000]) with adult snout-vent vent tube is median. Te tail fns originate at length (SVL) ranging between 44–62 mm the tail-body junction. Te tail fns are trans- (Table 1). Head width is generally greater parent, almost unspotted (Cei 1980). Indi- than head length and hind limbs are moder- viduals collected from the Bolivian Chaco ately short (Table 1). Leptodactylus bufonius possessed tail fns that were darkly pigment- lacks distinct dorsolateral folds. Te tarsus ed with melanophores, especially towards contains white tubercles, but the sole of the the terminal end of the tail (Christopher M. foot is usually smooth.