BIHAREAN BIOLOGIST 6 (1): pp.61-64 ©Biharean Biologist, Oradea, Romania, 2012 Article No.: 121107 http://biozoojournals.3x.ro/bihbiol/index.html

New records of Phanerotomella rufa (Marshall, 1898) and Phanerotoma (Phanerotoma) acuminata Szépligeti, 1908 (: ) from northern Iran

Samira FARAHANI1, Ali Asghar TALEBI1* and Ehsan RAKHSHANI2

1. Department of Agricultural Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O. Box: 14115-336, Tehran, Iran. 2. Department of Plant Protection, College of Agriculture, University of Zabol, Iran. * Corresponding author: A.A. Talebi, E-mail: [email protected]

Received: 22. October 2011 / Accepted: 10. April 2012 / Available online: 4. May 2012 / Printed: June 2012

Abstract. Two rare species, Phanerotomella rufa (Marshall, 1898) and Phanerotoma (Phanerotoma) acuminata Szépligeti, 1908 (Hymenoptera: Braconidae: ) have been recorded for the first time in Iran. P. rufa represents the first record of the genus Phanerotomella Szépligeti, 1900 in Iran. Specimens were collected using malaise traps from different habitats of northern Iran during 2010. Diagnostic characters and geographical distribution of the species are briefly discussed.

Key words: Cheloninae, Phanerotomini, new record, rare species, Iran.

Introduction then treated with 100% ethanol for 5 min. followed by hexamethyld- isilazane (HMDS) for 30 min and finally placed on the glass plate for Braconidae is one of the largest families of the parasitic drying (Heraty & Hawks 1998). The dried specimens were then card- wasps and is grouped into 48 subfamilies worldwide (Yu et mounted and labeled. The specimens were identified using reliable al. 2006), with a diverse habitat and biology (van Achterberg keys and comparing with the original descriptions (van Achterberg 1990). Geographical characteristics of the collecting sites are given in 1990, 1993, Shaw & Huddleston 1991). The Cheloninae is the Table 1. All specimens are deposited in the collection of the most easily recognizable subfamily of Braconidae, because of Department of Entomology, Tarbiat Modares University, Tehran. the metasomal carapace (formed by the fusion of the first three tergites), the complete posterior transverse carina on the mesosternum and three submarginal cells in the fore- wing. Members of the subfamily Cheloninae are cosmopoli- tan species that include the solitary egg-larval endoparasi- toids of , especially Tortricoidea and Pyraloidea, but also other taxa whose larvae are born in stems, buds or fruits (Shaw 1997). Cheloninae comprise more than 1300 de- scribed species worldwide (Yu et al. 2006, Edmardash et al. 2011). In spite of the worldwide distribution, only the tribes Chelonini Förster, 1862 and Phanerotomini Baker, 1926 are represented in the Palaearctic region (van Achterberg 1990, Yu et al. 2006, Aydogdu 2008). The western Palaearctic spe- cies of the Phanerotomini were revised and keyed by van Achterberg (1990), who reported a total of 47 species. To date, a total of 288 species of Phanerotomini have been re- ported in the world and approximately 50 species are found Figure 1. The habitat in which specimens in the Palaearctic region (Yu et al. 2006). The fauna of Iranian were collected by malaise trap. Braconidae was poorly studied. The first work mentioning some Cheloninae from Iran was that of Telenga (1941) who reported six species. Until recently, 27 species of Cheloninae Results and Discussion have been recorded from Iran (van Achterberg & Mehrnejad 2002, Ghahari et al. 2009, Fallahzadeh & Saghaei 2010, Gha- Two species belonging to the tribe Phanerotomini were iden- hari & Fischer 2011, Lashkari Bod et al. 2011), distinctly rep- tified and recorded for the first time from Iran. The genus resenting a small part of this large group of . Six spe- Phanerotomella has been reported here for the first time from cies of the genus Phanerotoma have already been known from Iran. Iran (Hedwig 1957, Zettel 1990, Al-e-Mansour & Mostafavi Phanerotomella rufa (Marshall, 1898) (Fig. 2-6) 1993, Fallahzadeh & Saghaei 2010, Ghahari & Fischer 2011). Here, we present two new records of the tribe Phanero- Material examined: Alborz province - Karadj (N: 35° 46.336', tomini from Iran. E: 50° 56.749', 1278m asl), 22-VI-2010, 1♂; 07-IX-2010, 1♀; Al- borz province - Shahriar (N: 35° 40.135', E: 50° 56.944', 1168m asl), 28-IX-2010, 1♂, leg. A. Nadimi. Material and Methods Diagnosis: body length 2.6 mm (for the Iranian speci-

mens), body yellowish or brown (Fig. 2); number of antennal Material for this study was collected from different habitats of the northern Iran using malaise traps during 2010 (Fig. 1). The speci- segments of male 32 (Fig. 4) and female 31; eyes compara- mens were extracted from the traps and sorted weekly. They were tively large (Fig. 6); ocelli small; frons nearly always with

62 Farahani, S. et al.

Table 1. Geographical characteristics of sampling locations in Guilan and Alborz provinces during 2010.

Locality Latitude and longitude Altitude (m) Habitat Alborz province, Karadj N= 35˚ 46.336' / E= 50˚ 56.749' 1278 Orchard Alborz province, Shahriar N= 35˚ 40.135' / E= 50˚ 56.944' 1168 Orchard Guilan province, Eshmankamachal N= 37˚ 22.061' / E= 49˚ 57.964' -1 Humid forests Guilan province, Rudsar, Orkom N= 36˚ 45.739' / E= 50˚ 18.798' 1201 Deciduous forests and hazelnut

Figures 2-6. The external morphology of adult male of Phanerotomella rufa, 2., dor- sal view of general habitus; 3. Forewing; 4. Antenna; 5. Dorsal aspect of metasoma; 6. Lateral aspect of head.

some rugose laterally and smooth medially; fore wing length of male and female 23 (Fig. 9); face rugose; mesosternum 2.4 mm, marginal cell of fore wing narrow and short, fore shiny, mesoscutum densely and finely rugose (Fig. 12); fore wings 2-SR and SR1 straight, 1-R1 subequal to pterostigma; wing length 4-4.5 mm, marginal cell of fore wing wide and parastigma small, second submarginal cell usually distinctly long, 2-SR and SRI straight; 1-R1 longer than pterostigma petiolate (Fig. 3); hind wing length 1.9-2 mm, vein cu-a of (1.3 time), parastigma large, dark; first discal cell pigmented, hind wing absent; legs yellowish; apex of third metasomal hind wing length 3.2-3.5 mm, subbasal cell of hind wing tergite of male with lateral pair of lamelliform-lobes (Fig. 5), open, SC+R1 of hind wing straight; middle tibia with con- first and second tergites reticulated; spicuous blister (Fig. 11); third metasomal tergite of female Distribution: Austria, Greece, Hungary, Italy, Turkey, narrowed distally (Fig. 10) and its lateral sides nearly USSR (Moldavia), Serbia (van Achterberg 1990, Yu et al. straight, metasoma strongly flattened. 2006, Zikic et al. 2010) and Iran (newly recorded). Distribution: Austria, Czech Republic, Slovakia, Eng- Biology: unknown. land, Italy, France, Netherlands, Romania, former USSR (Azerbajdzhan, Crimea), Germany, Serbia, Mongolia, Mon- Phanerotoma (Phanerotoma) acuminata Szépligeti, 1908 tenegro (van Achterberg 1990, Yu et al. 2006, Papp 2009, (Fig. 7-12) Zikic et al. 2010) and Iran (newly recorded). Material examined: Guilan province - Eshmankamachal (N: Biology: It has been reported as a parasitoid of the 37°22.061', E: 49°57.964', -1m asl), 05-VII-2010, 1♀; 11-VII- Clover hay-worm , costalis Fabricius, 1775 2010, 2♂; Guilan province - Rudsar, Orkom (N: 36˚45.739΄, E: (Lepidoptera: ) (Yu et al. 2006). 50˚18.198΄, 1201m asl) 06-VII-2010, 2♀, leg. M. Khayrandish. Very little information is available about of Diagnosis: body length 4-4.9 mm (for the Iranian speci- the tribe Phanerotomini in Iran. Until recently, six Phanero- mens), body yellowish (Fig. 7); number of antennal segments toma species have been reported from Iran. These are: Phan- New records of Phanerotomella rufa and Phanerotoma (Phanerotoma) acuminate from northern Iran 63 erotoma (Bracotritoma) parva Kokujev, 1903, P. (B.) masiana erotomella has been recorded from Greece (van Achterberg Fahringer, 1934, P. (P.) fracta Kokujev, 1903, P. (P.) leucobasis 1990), Turkey (Aydogdu & Beyarslan 2009), Russia Far East Kriechbaumer, 1894, P. (P.) syleptae Zettel, 1990 and P. (B.) (Belokobylskij 1986), India (Ahmad & Shujauddin 2003), Ko- permixtellae Fischer, 1968 (Hedwig 1957, Zettel 1990, Al-e- rea (Papp, 2003), Austria (Zettel, 1987) and Germany, Bohe- Mansour & Mostafavi 1993, Fallahzadeh & Saghaei 2010, mia, Hungary, Moldavia (Zettel, 1989). The genus Phanero- Ghahari & Fischer 2011). Recently, Ghramh (2011) has re- toma has also a wide distribution in Central Asia (van ported four species of the genus Phanerotoma from Saudi Achterberg 1990, Aydogdu & Beyarslan 2009) indicating a Arabia. The genus Phanerotomella has been recorded for the need for further studies in Iran, concerning their potential as first time from Iran. Many species have been described from biological control agents against some important lepidop- Afrotropical region (van Achterberg 1990). The genus Phan- teran pests.

Figures 7-12. The external morphology of adult female of Phanerotoma (P.) acuminata: 7. lateral view of general habitus; 8. Forewing; 9. An- tenna; 10. Dorsal aspect of metasoma; 11. Mid- dle tibia; 12. Dorsal view of mesosoma.

Acknowledgements. We would like to thank the department of Aydogdu, M. (2008): A new species of the genus Chelonus Panzer, 1806 Entomology, Tarbiat Modares University for providing financial (Hymenoptera: Braconidae: Cheloninae) from Western Anatolia (Turkey). support for this research. We are most grateful to the editor, Dr. Biologia 63(2): 245-248. Aydogdu, M., Beyarslan, A. (2009): A review of the tribe Phanerotomini Mehmet Zülfü Yildiz and an anonymous reviewer for their very (Hymenoptera, Braconidae, Cheloninae) in Turkey, with a new host record valuable comments and suggestions on the earlier version of this for Phanerotoma (Bracotritoma) permixtellae. Biologia 64(4): 748–756. paper. Belokobylskij, S.A. (1986): Phanerotomella Szépligeti, 1900 – New for the fauna of Far East USSR, Insecta-Braconidae-Cheloninae (Hym., Braconidae). Hymenoptera of East Siberia and Far East, Vladivostok: 41–48. Edmardash, Y.A.E., Abdel-Dayem, M.S., Gadallah, N.S. (2011): The subfamily References Cheloninae (Hymenoptera, Braconidae) from Egypt, with the description of two new species. ZooKeys 115: 85-102. Ahmad, Z., Shuja-Uddin (2003): Description of a new species of Phanerotomella Fallahzadeh, M., Saghaei, N. (2010): Checklist of Braconidae (Insecta: (Hymenoptera: Braconidae) from India. Journal of Entomological Research Hymenoptera) from Iran. Munis Entomology & Zoology 5(1): 170–186. 27(4): 353–355. Ghahari, H., Fischer, M. (2011): A contribution to the Braconidae (Hymenoptera Al-e-Mansour, H., Mostafavi, M. S. (1993): The first record of Braconidae bees ) from north-western Iran. Calodema 134: 1–6. on forest and range vegetation in the Fars province. Proceeding of 11th Ghahari, H., Gadallah, N.S., Cetin Erdogan, O., Hedqvist, K. J., Fischer, F., Iranian Plant Protection Congress, University of Guilan, p. 236. Beyarslan, A., Ostovan, H. (2009): Faunistic note on the Braconidae 64 Farahani, S. et al.

(Hymenoptera: Ichneumonoidea) in Iranian cotton fields and surrounding van Achterberg, C. (1993): Illustrated key to the subfamilies of the Braconidae. grasslands. Egyptian Journal of Biological Pest Control 19: 115-118. (Hymenoptera: Ichneumonoidea). Zoologische Verhandelingen (Leiden) Ghramh, H.A. (2011): Description of a new species of the genus Phanerotoma 283: 1–189. Wesmael (Hymenoptera: Braconidae) and a key to Saudi Arabian species. van Achterberg, C., Mehrnejad, M.R. (2002): The braconid parasitoids African Journal of Biotechnology 10(65): 14649-14651. (Hymenoptera: Braconidae) of Kermania pistaciella Amsel (Lepidoptera: Hedwig, K. (1957): Ichneumoniden und Braconiden aus den Iran 1954 Tineidae: Hieroxestinae) in Iran. Zoologische Mededelingen (Leiden) 76(2): (Hymenoptera). Jahresheft des Vereins für Vaterlaendische Naturkunde 27-39. 112(1): 103–117. Yu D.S., van Achterberg, C., Horstmann, K. (2006): World Ichneumonoidea Heraty, J.M., Hawks, D. (1998): Hexamethyldisilazane: A chemical alternative 2005. Taxonomy, Biology, Morphology and Distribution (Braconidae). for drying insects. Entomological News 109: 369-374. Taxapad 2006 (Scientific Names for Information Management) Interactive Lashkari Bod, A., Rakhshani, E., Talebi, A.A., Lozan, A., Žikić, V. (2011): New Catalogue on DVD/CDROM. Vancouver. Records of Cheloninae (Förster, 1862) and Braconinae (Nees, 1811) (Insecta: Zettel, H. (1987): Beitrag zur kenntnis der Sigalphinen-und Cheloninen fauna in Hymenoptera: Braconidae) from Iran. Checklist 7: 632-634. Osterreich (Hymenoptera, Braconidae). Linzer biologische Beiträge 19(2): Papp, J. (2003): Braconidae (Hymenoptera) from Korea, XXI. species of fifteen 359-376. subfamilies. Acta Zoologica Academiae Scientarum Hungaricae 49(2): 115- Zettel, H. (1989): Die gattung Phanerotomella Szépligeti (Hymenoptera: 152. Braconidae: Cheloninae). Linzer Biologische Beiträge 21: 15-142. Papp, J. (2009): Braconidae (Hymenoptera) Mongolia, XVII. Eleven subfamilies. Zettel, H. (1990): Description of four new Phanerotoma species from eastern Asia Acta Zoologica Academiae Scientarum Hungaricae 55(2): 139–173. with a key for identifying the oriental species (Hymenoptera: Braconidae, Shaw, S.R., (1997): Subfamily Cheloninae: 193-201 In: Wharton, R.A., Marsh, Cheloninae). Zeitschrift der Arbeitsgemeinschaft Österreichischer P.M., Sharkey, M.J. (eds), Manual of the New World genera of the family Entomologen 42(3–4): 110–120. Braconidae (Hymenoptera). Special Publication of the International Society Zikic, V., van Achterberg, K., Stankovic, S. (2010): A contribution to Braconidae, of Hymenopterists 1: 1–439. Hybrizontidae (Hymenoptera: Ichneumonoidea) and Stephanidae Shaw, M.R., Huddleston, T. (1991): Classification and Biology of Braconid (Hymenoptera: Stephanoidea) from the South-West Balkans. Acta Wasps (Hymenoptera: Braconidae). British Museum of Natural History, Entomologica Serbica 15(2): 227-235. London. Telenga, N.A. (1941): Family Braconidae, subfamily Braconinae (continuation) and Sigalphinae. Fauna USSR. Hymenoptera 5(3), Moskva-Leningrad: Akademiya nauk SSSR. 466 pp. van Achterberg, C. (1990): Revision of the western Palaearctic Phanerotomini (Hymenoptera: Braconidae). Zoologische Verhandelingen (Leiden), 255: 1– 106.