Exploring and Potentiating Human Bone Marrow Derived Mesenchymal Stem Cells [hBMSCs] and Differentiated Islets for Effective Diabetes Therapy ABBREVIATION
LIST OF ABBREVIATION AF555-Alexa Fluor 555 EBs- Embryoid bodies AGE- advanced glycosylation end-product EDTA- Ethylenediaminetetraacetic acid aPKC- Atypical protein kinase C EGF-Epidermal growth factor ANOVA-Analysis of variance EGFR- epidermal growth factor receptor Arx- Aristaless-related homeobox gene EL- Enicostemma littorale ATP- Adenosine triphosphate ELISA- Enzyme linked immunosorbent bHLH- Basic helix–loop–helix assay BM- Bone marrow EMT - epithelial–mesenchymal transition BMC- Bioactive molecules cocktail ESCs- Embryonic stem cells BMP-bone morphogenic protein FACS- Fluorescent activated cell sorter BSA- Bovine serum albumin FBS- Fetal Bovine Serum cAMP- Cyclic Adenosine monophosphate FDA- Food and Drug Administration
CaCl2 –Calcium chloride FGF- Fibroblast growth factor CD-Cluster of differentiation FITC- Fluorescein isothiocyanate cDNA –Complementary DNA FOXO1-Forkhead box protein O1 ChIP-Chromatin immunoprecipitation FOXA2 - Forkhead box protein A2 CCl4- Carbon tetrachloride GAD- Glutamate decarboxylase CDK- Cyclin dependent kinase GATA4 -(GATA Binding Protein 4) CK-Cytokeratin GATA6- (GATA Binding Protein 6) CNS- Central Nervous System GCK- Glucokinase CPCSEA- Committee for the purpose of GFP- Green Fluorescent protein control and supervision of experiments on GLP-1- Glucagon-like peptide-1 animals GLUT- Glucose Transporter DAPI- 4’6’-diamidino-2-phenylindole GPx- Glutathione peroxidase Dihydrochloride GSH- Reduced glutathione DCFDA- 2',7' –dichlorofluorescin diacetate GSIS- Glucose-stimulated insulin secretion DM-Diabetes mellitus GTT- Glucose tolerance test DMEM- KO -Dulbecco’s Modified Eagle H & E- Hematoxylin and Eosin Media-Knock out hBMSCs: Human bone marrow-derived DMSO- Dimethyl Sulphoxide mesenchymal stem cell DTZ- Dithizone HGF- Hepatocyte Growth Factor
Mitul Vakani. Ph.D. Thesis 2020 Exploring and Potentiating Human Bone Marrow Derived Mesenchymal Stem Cells [hBMSCs] and Differentiated Islets for Effective Diabetes Therapy ABBREVIATION
HFM- Hollow fiber membrane LNA- Locked nucleic acid HNF- Hepatocyte nuclear factor MafA- musculoaponeurotic fibro sarcoma HNF-1 beta - hepatocyte nuclear oncogene homology A factor 1 homeobox B) MafB- musculoaponeurotic fibro sarcoma oncogene homology B HLA-Human leukocyte antigen MHC- Major histocompatibility complex HSCs-Hematopoietic stem cells MSCs- Mesenchymal stem cells IP-Immunoprecipitation MTT- (3-(4,5-Dimethylthiazol-2-yl)- I.P.- Intraperitoneal 2,5Diphenyltetrazolium Bromide) I.V. Intravenous NGN-3-Neurogenin-3 ICC- Immunocytochemistry NIDDM- Non-insulin dependent diabetes IFG-Impaired fasting glucose mellitus IGT- Impaired Glucose Tolerance NO- Nitric Oxide IHC- Immunohistochemistry NOD- Non-obese diabetic IL- Interleukin NEUROD1- Neuronal differentiation 1 ILCC- Islet like cell clusters NKX 6.1 -NK6 homeobox 1 ILC-Islet like clusters NKX2-2 NK2 homeobox 2 IPC-Insulin producing clusters NANOG-Homeobox protein NANOG INF- Interferon ONS- Oct4, Sox-2, Nanog INS- insulin gene Oct-4-Octamer binding transcription factor INGAP-Islet neogesis associated protein 4 IPC- Intra-Islet Precursor Cells PARG- PAR glycohydrolase iNOS-Inducible nitric oxide synthase PARP- Poly (ADP-ribose) Polymerase iPSC- Induced pluripotent stem cells PAX-4-Paired box-containing gene-4 IR- Insulin receptor PANC-1-Pancreatic carcinoma cell line IRS- Insulin receptor substrate PBS- Phosphate buffer saline PCR- Polymerase chain reaction ITS-Insulin transferrin selenium PCSK1- Proprotein Convertase kDa- Kilodaltons Subtilisin/Kexin Type 1 KGF- Keratinocyte growth factor PDL- Partial duct ligation KRBH- Krebs-Ringer bicarbonate hepes PDX-1- Pancreatic duodenal homeobox-1 buffer PE- Phycoerythrin KLF4- Kruppel-like factor 4
Mitul Vakani. Ph.D. Thesis 2020 Exploring and Potentiating Human Bone Marrow Derived Mesenchymal Stem Cells [hBMSCs] and Differentiated Islets for Effective Diabetes Therapy ABBREVIATION
PFA- Paraformaldehyde SIRT-1- Sirtuin1 PI- Propidium iodide SSB- Single strand DNA breaks PI3-K- phosphatidylinositol 3-kinase SST- Somatostatin PKB- Protein kinase B STZ- Streptozotocin PP-Pancreatic Polypeptide SYBR- Synergy Brands PPY-Polypyrrole T1DM- Type 1 diabetes mellitus PPAR-γ- Peroxisome proliferative T2DM- Type 2 diabetes mellitus activated TBARS-Thiobarbituric acid reactive receptor gamma substances PREPs-Pancreatic resident endocrine TF-Transcription factors Progenitors TGF-β- Transforming growth factor-β Psf-TPSG-Polysulfones D-ɑ-tocopheryl TGS-Transcriptional gene silencing polyethylene glycol 1000 succinate TNF- Tumor necrosis factor PPx- Partial pancreatectomy TRITC-Tetramethyl Rhodamine Iso- PTGS- Posttranscriptional gene silencing Thiocyanate qPCR- Quantitative PCR TSS-Transcription start site RISC- RNA-induced silencing complex TLDA-TaqMan Low density Array RFX6- Regulatory factor X6 UCB- Umbilical cord blood Reg- Regenerating islet-derived gene VEGF-Vascular endothelial growth factor family
RNU6- RNA, U6 Small Nuclear-1 ROS-Reactive oxygen species RT-PCR- Reverse transcriptase PCR SFM- Serum free media SEM-Standard error of mean shRNA-Short hairpin RNA Shh-Sonic hedgehog siRNA-Small interfering RNA SOD- superoxide dismutase SOX2- SRY (sex determining region Y)- box 2 SOX17- SRY-Box Transcription Factor 17
Mitul Vakani. Ph.D. Thesis 2020