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Genome Characterization of the Snail Biomphalaria Glabrata: Lophotrochozoan Biology and Transmission of Schistosomiasis

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Genome Characterization of the Snail Biomphalaria Glabrata: Lophotrochozoan Biology and Transmission of Schistosomiasis 1 Whole genome analysis of a schistosomiasis-transmitting freshwater snail 2 Adema, Coen M.; Hillier LaDeana W.; Jones, Catherine S.; Loker, Eric S.; Knight, Matty; Minx, 3 Patrick; Oliveira, Guilherme; Raghavan, Nithya; Shedlock, Andrew; Amaral, Laurence Rodrigues 4 do; Arican-Goktas, Halime D.; Assis, Juliana; Baba, Elio Hideo; Baron, Olga Lucia; Bayne, 5 Christopher J.; Bickham-Wright, Utibe; Biggar, Kyle K.; Blouin, Michael; Bonning, Bryony C.; 6 Botka, Chris; Bridger, Joanna M.; Buckley, Katherine M.; Buddenborg, Sarah K.; Caldeira, Roberta 7 Lima; Carleton, Julia; Carvalho, Omar S.; Castillo, Maria G.; Chalmers, Iain W.; Christensens, 8 Mikkel; Clifton, Sandy; Cosseau, Celine; Coustau, Christine; Cripps, Richard M.; Cuesta-Astroz, 9 Yesid; Cummins, Scott; di Stephano, Leon; Dinguirard, Nathalie; Duval, David; Emrich, Scott; 10 Feschotte, Cédric; Feyereisen, Rene; FitzGerald, Peter; Fronick, Catrina; Fulton, Lucinda; Galinier, 11 Richard; Geusz, Michael; Geyer, Kathrin K.; Giraldo-Calderon, Gloria; Gomes, Matheus de Souza; 12 Gordy, Michelle A.; Gourbal, Benjamin; Grossi, Sandra; Grunau, Christoph; Hanington, Patrick C.; 13 Hoffmann, Karl F.; Hughes, Daniel; Humphries, Judith; Izinara, Rosse; Jackson, Daniel J.; Jannotti- 14 Passos, Liana K.; Jeremias, Wander de Jesus; Jobling, Susan; Kamel, Bishoy; Kapusta, Aurélie; 15 Kaur, Satwant; Koene, Joris M.; Kohn, Andrea B; Lawson, Dan; Lawton, Scott P.; Liang, Di; 16 Limpanont, Yanin; Lockyer, Anne E.; Lovato, TyAnna L.; Liu, Sijun; Magrini, Vince; McManus, 17 Donald P.; Medina, Monica; Misra, Milind; Mitta, Guillaume; Mkoji, Gerald M.; Montague, 18 Michael J.; Montelongo, Cesar; Moroz, Leonid L; Munoz-Torres, Monica C.; Niazi, Umar; Noble, 19 Leslie R.; Pais, Fabiano; Papenfuss, Anthony T.; Peace, Rob; Pena, Janeth J: Pila, Emmanuel A.; 20 Quelais, T; Raney, Brian J.; Rast, Jonathan P.; Ribeiro, Fernanda; Rollinson, David; Rotgans, 21 Bronwyn; Routledge, Edwin J.; Ryan, Kathryn M.; Scholte, Larissa; Silva, Francislon; Storey, 22 Kenneth B; Swain, Martin; Tennessen, Jacob A.; Tomlinson, Chad; Trujillo, Damian L.; Volpi, 23 Emanuela V.; Walker, Anthony J.; Wang, Tianfang; Wannaporn, Ittiprasert; Warren, Wesley C.; 24 Wu, Xiao-Jun; Yoshino, Timothy P.; Yusuf, Mohammed; Zhang, Si-Ming; Zhao, Min; Wilson, 25 Richard K. 26 27 Summary 28 Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni. 29 With the World Health Organization's goal to eliminate schistosomiasis as a global health problem 30 by 2025, there is now renewed emphasis on snail control. Our characterization of the genome of 31 Biomphalaria glabrata, a lophotrochozoan protostome, provides timely and important information 32 on snail biology. We describe phero-perception, stress resistance, immune function and multi-level 33 regulation of gene expression that support the persistence of B. glabrata in aquatic habitats and 34 define this species as a suitable snail host for S. mansoni. We identify several potential targets for 35 developing novel control measures aimed at reducing snail-mediated transmission of 36 schistosomiasis. 37 38 The fresh water snail Biomphalaria glabrata (Lophotrochozoa, Mollusca) is of medical relevance as 39 this Neotropical gastropod contributes as obligate intermediate host of Schistosoma mansoni 40 (Lophotrochozoa, Platyhelminthes) to transmission of the neglected tropical disease (NTD) human 41 intestinal schistosomiasis1. An S. mansoni miracidium initiates a chronic parasite-snail infection that 42 alters B. glabrata immunity and metabolism, causing parasitic castration such that the snail does not 43 reproduce but instead supports generation of cercariae, the human-infective stage of S. mansoni. 44 Patently infected snails continuously release free-swimming cercariae that penetrate the skin of 45 humans that they encounter in their aquatic environment. Inside the human definitive host, S. 46 mansoni matures to adult worms that reproduce sexually in the venous system surrounding the 47 intestines, releasing eggs, many of which pass through the intestinal wall and are deposited in water 48 with the feces. Miracidia hatch from the eggs and complete the life cycle by infecting another B. 49 glabrata. Related Biomphalaria species transmit S. mansoni in Africa. Schistosomiasis is chronically 50 debilitating; estimates of disease burden indicate that disability-adjusted life years (DALYs) lost due 51 to morbidity rank schistosomiasis second only to malaria among parasitic diseases in impact on 52 global human health2. 53 In the absence of a vaccine, current control measures emphasize mass drug administration (MDA) of 54 praziquantel (PZQ), the only drug available for large-scale treatment of schistosomiasis3. 55 Schistosomes, however, may develop resistance and reduce the effectiveness of PZQ4. Importantly, 56 PZQ treatment does not protect humans against rapid re-infection following exposure to water-borne 57 cercariae released from infected snails. Snail-mediated parasite transmission must be interrupted to 58 achieve long-term sustainable control of human schistosomiasis5. The World Health Organization 59 has set a strategy for the global elimination of schistosomiasis as a public health threat by the year 60 2025 that recognizes both MDA approaches and targeting of the snail intermediate host as crucial 61 toward achieving this goal6. This significant undertaking provides added impetus for detailed study 62 of B. glabrata. 63 Genome analyses of B. glabrata also provide evolutionary insights by increasing the relatively few 64 taxa of the Lophotrochozoa that have been characterized. To date a majority of lophotrochozoan 65 genomes have been derived from parasitic, sessile, or marine animals (i.e. platyhelminths, leech, 66 bivalve, cephalopod, polychaete)7-11. Thus, B. glabrata likely possesses novel genomic features that 67 characterize a free-living lophotrochozoan from a freshwater environment. Here we characterize the 68 B. glabrata genome and describe biological properties that enable the snail’s persistence in aquatic 69 environments and define this snail as a suitable host for S. mansoni, including aspects of immunity 70 and gene regulation. These efforts, we anticipate, will foster developments to interrupt snail- 71 mediated parasite transmission in support of schistosomiasis elimination. 72 The B. glabrata genome comprises eighteen chromosomes (Supplementary Text 1 and 73 Supplementary Fig. 1-3) and has an estimated size of 916Mb12. We assembled the genome of BB02 74 strain B. glabrata13 from short reads, Sanger sequences, and 454 sequence data (~27.5X coverage) 75 and used a linkage map to assign genomic contigs to linkage groups (Supplementary Text 2 and 76 Supplementary Table 1). We captured transcriptomes from twelve different tissues (Illumina 77 RNAseq) and mapped these reads to the assembly to aid annotation (Supplementary Text 3, 78 Supplementary Figs. 4-8 and Supplementary Tables 2-7). 79 Olfaction in an aquatic environment 80 Aquatic molluscs employ proteins for communication; e.g. Aplysia attracts conspecifics using water- 81 soluble peptide pheromones14. We collected B. glabrata proteins from snail conditioned water 82 (SCW) and following electrostimulation (ES), which induces rapid release of proteins. NanoHPLC- 83 MS/MS identified 177 secreted proteins shared among 533 proteins from SCW and 232 proteins 84 from ES (Supplementary Table 8). Detection of an ortholog of temptin, which plays a key role in 85 pheromone attraction of Aplysia15, suggests an operational pheromone sensory system in B. 86 glabrata. To gain insight into the mechanisms for pheromone perception, we analyzed the B. 87 glabrata genome for olfactory G-protein coupled receptor (GPCR)-like supergene families and 88 identified 242 seven transmembrane domain GPCR-like genes belonging to fourteen subfamilies that 89 are clustered in the genome (Supplementary Text 4, Supplementary Figs. 9,10 and Supplementary 90 Table 9). RT-PCR and in situ hybridization confirmed expression of a GPCR-like gene within the 91 mollusc’s tentacles, known to be involved in chemosensation (Figure 1). This communication 92 system allows B. glabrata to detect conspecifics in aquatic environments but may have a tradeoff 93 effect by potentially exposing the snail as a suitable target for pathogens. 94 95 Fig 1. Candidate chemosensory receptors of B. glabrata. (A) Scaffold 39 contains fourteen 96 candidate chemosensory receptor genes (BgCRa-n). Most encode 7-transmembrane domain proteins, 97 BgCRm and BgCRn are truncated to six-transmembrane domains. Genes occur in both directions. 98 (B) Phylogenetic analysis of scaffold 39 chemosensory receptors. (C) Schematic of receptor showing 99 conserved and invariable amino acids, transmembrane domains I-VII; and location of glycosylation 100 sites. (D) Scanning electron micrograph showing anterior tentacle, with cilia covering the surface. 101 (B) RT-PCR gel showing amplicon for BgCR509a and actin from B. glabrata tentacle. (F, G) In situ 102 hybridization showing sense (negative control) and antisense localization of BgCR509a in anterior 103 tentacle section (purple). 104 105 Stress responses and immunity 106 Biomphalaria glabrata endures environmental insults, including biotic and abiotic stress from heat, 107 cold, xenobiotics, pollutants, injury and infection. Additional to previous reports of Capsaspora16 as 108 single-cell eukaryote endosymbiont, we noted from the sequenced material an unclassified 109 mycoplasma or related mollicute bacteria
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