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ACHE COL4A1 ACTG1 ANXA5 ACTG1 GSN ACTG1 TMSB4X ACTG1 TMSB4Y ACTG1 PTPRO ACTG1 CFL1 ACTG1 PLD1 ACTG1 FHOD1 ACTG1 CAP2 ACTA1 GSN ACTA1 PRKCE ACTA1 TNNI1 ACTA1 VCL ACTA1 TMSB4X ACTA1 MYLK ACTA1 TJP1 ACTA1 CFL1 ACTA1 MYO10 ACTA1 AIF1 ACTA1 PLD1 ACTA1 PAK1 ACTA1 CORO2B ACTA1 FHOD1 ACTA1 CLIC5 ACTA1 CCT5 ACTA1 S100A4 ACTA1 PARVA ACTB ACTG1 ACTB VIL2 ACTB NT5E ACTB EEF1A1 ACTB GSN ACTB LMNA ACTB PTN ACTB LYN ACTB TMSB4X ACTB CFL1 ACTB MYO1E ACTB PLD1 ACTB HNRPAB ACTB SMARCA4 ACTB CPNE2 ACTB CCT5 ACTB ARPC1B ACTB TPM2 AK1 FHL2 ADCY1 GNAI2 ARF1 CHRM3 ARF1 PLD1 ARF1 COPE ADM MME ADM CALCRL A2M B2M A2M CTSB A2M PAEP A2M KLK3 A2M PDGFB A2M ADAM19 A2M NGFB APLP1 TMSB4X APLP1 TCF21 APLP1 TSC22D1 ANXA3 EMG1 CD38 PECAM1 ARRB1 FLNA ARRB1 NSF NPR1 PRKG1 AXL IL2RG AXL NCK2 AXL TENC1 CD40 MAP3K8 CD40 JAK3 B2M HLA-E B2M CD1D CEACAM1 FLNA BMP2 ENG BMP2 MGP BMP2 TGFB1 BMP2 BMPER CDH2 CTNNB1 CDH2 CREBBP CDH2 BOC CALCA RAMP2 CALCA CALCRL CALM1 CSNK2A1 CALM1 CLTB CALM1 HSP90AA1 CALM1 ITPKB CALM1 RRAD CALM1 RAB3B CALM1 RGS2 CALM1 MYLK CALM1 MYO10 CALM1 NRGN CALM1 RGS4 CALM1 RGS10 CALM1 PLCD1 CALM2 RAB3B CALM3 RAB3B CALM3 AKAP9 CPM EGF CSNK2A1 CTNNB1 CSNK2A1 TOP1 CSNK2A1 EGR1 CSNK2A1 FGF1 CSNK2A1 HSP90AA1 CSNK2A1 IFI16 CSNK2A1 FOS CSNK2A1 MYCN CSNK2A1 JUN CSNK2A1 SPP1 CSNK2A1 RRAD CSNK2A1 MEF2C CSNK2A1 UBTF CSNK2A1 PSEN2 CSNK2A1 PAK1 CSNK2A1 RNF7 CSNK2A1 HSPB8 CSNK2A1 PSMA4 CSNK2A1 PLEKHO1 CTNNB1 VIL2 CTNNB1 FYN CTNNB1 FLT1 CTNNB1 PECAM1 CTNNB1 PTPRM CTNNB1 TGFBR2 CTNNB1 KDR CTNNB1 PTPN13 CTNNB1 PSEN2 CTNNB1 FHL2 CTNNB1 SMARCA4 CTNNB1 DLG5 CTNNB1 TCF7L1 CTNNB1 BOC CTNNB1 MAGI2 CTNNB1 SOX17 CTSB REN CTSB RGS2 CEBPD CREBBP CDKN1A CSNK2A1 CDKN1A GADD45A CDKN1A PCNA CDKN1A GADD45B CDKN1A GADD45G CDKN1A CCDC85B CDKN1A IBRDC2 CDC25B CSNK2A1 CDC25B CREBBP CDC25B YWHAZ CHRM3 GNAQ CLTB CSNK2A1 CLTB HIP1 SSFA2 LUC7L2 COL4A3 CD93 COL4A3 DCN COL4A3 SERPINE2 COL4A1 CD93 COL4A1 DCN COL4A1 NID1 COL4A1 SERPINE2 COL4A4 CD93 COL4A4 DCN COL4A4 SERPINE2 COL13A1 NID1 COL13A1 SPARC COL13A1 NID2 MME VIP CTGF TGFB1 CTGF VEGFA GJA1 GJA3 GJA1 PRKCE GJA1 TJP1 GJA1 GJA5 GJA3 TJP1 VIL2 SLC9A3R2 VIL2 CLIC5 VIL2 SCYL3 VIL2 PALLD CD55 FYN DCN TGFB1 DCN FLNA GADD45A PCNA EGR1 JUN EGR1 NAB1 EGR1 NAB2 EEF1A1 PAEP EEF1A1 PLAUR EEF1A1 WARS EEF1A1 PAFAH1B3 EEF1A1 EIF3S5 EEF1A1 GADD45G EEF1A1 KIF1B EEF1A1 C11orf79 EEF1A1 STMN2 EEF1A1 POLR2C EEF1A1 ORMDL3 ENG TGFB1 ENG TGFBR2 ENG TGFBR3 SELE VCL FGF1 S100A13 FGF1 NRP1 FGF1 RPS2 FGF1 FGFBP1 ANXA5 ITGB5 ANXA5 KDR EDNRA GNAI2 GATA3 LMO2 GATA3 LMO1 GATA3 TAL1 EGF LMNA EGF ADAM12 EPOR LYN EPOR KITLG EPOR SOCS3 EPOR STAT5B EPOR GRAP EPOR GAB2 EPOR INPP5D EPOR SH2B2 F3 FLNA FNTA TGFB1 FNTA PTP4A3 FAS CALM1 FAS VIL2 FAS EEF1A1 FAS FYN FAS RAP1A FAS UBE1L FAS PTPN13 FAS CD47 FAS TNFSF13 FTH1 GOLPH2 ITGA5 ITGB1 ITGA5 ITGB5 ITGA5 SPP1 ITGA5 PECAM1 ITGB1 LGALS1 ITGB1 SPP1 ITGB1 PRKCE ITGB1 VCAM1 ITGB1 TSPAN4 ITGB1 MAP4K4 ITGB1 ITGB1BP1 ITGB1 ITGA8 FLT4 ITGA5 FLT4 ITGB1 FLT4 KDR FYN MCAM FYN PLAUR FYN PDGFRB FYN PECAM1 FYN PRKCE FYN SDC3 FYN KDR FYN TEK FYN TYRO3 FYN STAT1 FYN LCP2 FYN K-ALPHA-1 FYN NPHS1 FYN TRPC6 FYN GRAP FYN PRKCH FYN ADAM15 FYN SH2B2 FYN DOK4 GSN VCL GSN CLIC5 GNAI2 RGS12 GNAI2 RGS4 GNAI2 RGS5 GUCY1B3 HSP90AA1 HSP90AA1 NOS3 HSP90AA1 KDR HSP90AA1 NR2C2 HSP90AA1 MAP3K3 HSP90AA1 ST13 HLA-DRA HLA-DRB1 ICAM2 VIL2 ICAM2 MSN IGFBP5 SPP1 IGFBP5 SERPINE1 IGFBP5 ADAM12 IGFBP5 PAPPA2 ITPR3 TRPC6 ITPR3 TRPC4 ITPR3 TRPC1 ITGB5 MYO10 ITGB5 PALLD ITGB5 TENC1 ISG15 USP18 IRF1 STAT1 IL6 IL6ST LAMC1 NID1 LAMC1 NID2 LMNA TMPO LMNA PCBP1 LMNA SYNE1 LMNA TOR1AIP1 ANXA1 DYSF ANXA2 CEACAM1 ANXA2 S100A6 ANXA2 CTSB ANXA2 PLAT ANXA2 DYSF ANXA2 S100A4 ALOX12 LMNA LOR VIM MAP4 MARK1 MAP4 MARK4 PTN SDC1 PTN SDC3 PTN SNCAIP PTN EIF3S5 PTN GADD45G PTN MYCBP PTN RIT1 PTN LUC7L2 NOS3 NOSTRIN HMGB1 PLAT HMGB1 MECP2 HMGB1 TLR4 HMGB1 CTCF HMGB1 SOX18 NFKB2 RELB EBF1 ZNF423 ETS1 GATA3 ETS1 ETS2 ETS1 FOS ETS1 JUN ETS1 STAT6 ETS1 STAT5B AKT2 HSP90AA1 ETS2 GATA3 ETS2 JUN ETS2 SMARCA4 ETS2 STAT5B FOS LMNA FOS JUN FOS JUNB FOS STAT1 FOS SUMO3 NOTCH4 PSEN2 NOTCH4 DLL4 NOTCH4 MAML1 FLT1 FYN FLT1 KDR FLT1 VEGFA FLT1 STAT1 FLT1 PGF FLT1 NRP1 RRAS RASIP1 LYN MME LYN PECAM1 LYN TEK LYN NPHS1 LYN GAB2 LYN SH2B2 JUN TOP1 JUN SKI JUN NFYA JUN STAT1 JUN SUMO3 JUN MAPK11 JUN PRKD1 JUN MAFB JUN TPM2 JUN SNRK RHOB FNTA RHOB ARHGEF3 SERPINE1 PLAT PLAT SPARC PLAT CLEC3B PDGFRB STAT1 PDGFRB STAT5B PDGFRB NCK2 PECAM1 PRKCE PCNA GADD45G KLK3 EPOR PROS1 TYRO3 PTPRB TEK EPHA2 TNFAIP1 EPHA2 SLA PRKCE AKAP9 PRKCE PRKD1 RRAD YWHAZ RRAD TPM2 RAP1A TCEB3 RAP1A SMARCA4 RAP1A RASA3 RAP1A RASGRP2 RAP1A RAPGEF5 RAP1A RASIP1 RAP2A MAP4K4 RAP2A RAPGEF5 RAP2A RASIP1 CLIP1 CLASP2 LMO2 TAL1 LMO2 STAT1 LMO2 JARID1A SPARC TGFB1 SPARC VEGFA KITLG PIK3CD VAMP2 STX11 VAMP2 VAPB LMO1 TAL1 TNC ITGA5 TNC PTPRB F2R GNAI2 TBXA2R YWHAZ TBXA2R RAB11A NFYA ZHX2 CEBPB CEBPD CEBPB EGR1 CEBPB HMGB1 CEBPB CREBBP CEBPB STAT6 CEBPB SMARCA4 E2F1 SP3 FES JAK3 FES RASA3 PDGFB PDGFRB TGFB1 TGFBR2 TGFB1 TGFBR3 TGFB1 EIF3S2 TGFB1 VASN TGFBR2 ARHGEF6 TGFBR2 TGFBR3 TGFBR2 EIF3S2 NOTCH1 PSEN2 NOTCH1 DLL4 NOTCH1 MAML1 TNNI1 CCDC85B MAP3K8 NFKB2 KDR TIMP3 KDR VEGFA KDR STAT1 KDR NRP1 UBE1L ISG15 VCAM1 VIL2 VEGFA NRP1 VIM YWHAZ VIM MICAL1 VIM PDLIM1 VIM SIAHBP1 VCL CORO2B C1R C1S CYBA NCF2 NCF2 S100A8 LPL UBE2Z LPL LUC7L2 MECP2 SKI FLNA SELE FLNA ITGB1 FLNA ITGB5 FLNA LMNA FLNA PSEN2 FLNA SH2B3 EFNB1 PTPN13 EFNB1 EPHB1 EFNB1 RGS3 EFNB1 NCK2 FHL1 FHL2 ARHGEF6 PARVB CRLF2 TSLP CHM RAB3B CHM RAB18 BGN TGFB1 IL2RG JAK3 IL2RG STAT1 MSN CD93 MSN VIL2 MSN VCAM1 SNTB2 DGKZ PTPN12 TGFB1I1 INPP5A YWHAZ CREBBP CSNK2A1 CREBBP CTNNB1 CREBBP EGR1 CREBBP NAP1L1 CREBBP EBF1 CREBBP ETS1 CREBBP ETS2 CREBBP JUN CREBBP EVI1 CREBBP E2F1 CREBBP RPS6KA3 CREBBP STAT1 CREBBP UBTF CREBBP STAT6 CREBBP FHL2 CREBBP RBBP4 CREBBP CITED2 CREBBP IRF7 CREBBP KLF13 CREBBP MAML1 CREBBP WT1 CREBBP NFIC CREBBP SERTAD1 CREBBP SERTAD3 CREBBP DACH1 JAK3 SOCS3 JAK3 SH2B2 DUSP3 EGF DUSP3 STAT1 AES HMGB1 AES PRDM1 AES SNCAIP AGER HMGB1 TEK TIE1 TEK DOK4 HIRA ASF1A PTPN13 YWHAZ ID3 IFI16 GPC1 VEGFA PPARD GADD45G PPARD HDAC7A FABP4 VIM EFNB2 PECAM1 EFNB2 PTPN13 EFNB2 EPHB1 EFNB2 RGS3 EFNB2 NCK2 STAT1 STAT5B PSME1 VIM PSME1 VCL PSME1 EMG1 MEF2C NOTCH1 MEF2C SOX18 MEF2C VGLL4 UBTF SMURF1 PTBP1 QKI IL6ST PLAUR IL6ST OSMR IL6ST SOCS3 DUSP1 MAPK12 GLG1 SELE GLG1 NFKB2 MMP14 SDC1 MMP14 TIMP2 MMP14 TIMP3 MMP14 SPOCK1 PSEN2 RAB11A CDKN1C PCNA CDKN1C NR4A2 GRK5 TBXA2R MYLK PAK1 KCNJ8 ABCC9 GNAQ TBXA2R GNAQ KDR GNAQ RGS2 GNAQ RGS4 GNAQ RGS5 TJP1 CLDN5 TJP1 JAM2 TJP1 KIRREL TJP1 TRPC4 VCP DERL1 FOXC1 HMGB1 MPP3 RPS2 MPP3 RAB31 CDH5 CTNNB1 CDH5 PTPRB CDH5 EPHA2 CDH5 KDR CDH5 VIM CDH5 PKP4 CDH5 SMG1 CDH5 P2RX4 PGF VEGFA PGF NRP1 SEMA3F VEGFA SEMA3F NRP1 RAN GADD45G RAN IPO11 CRIP2 PTPN13 CRIP2 PRKG1 PCBP1 PTBP1 PCBP1 SFRS3 PCBP1 PABPC1 PCBP1 QKI PCBP1 PCBP4 PCBP1 SIAHBP1 CD97 CD55 ACVRL1 TGFB1 ACVRL1 TGFBR2 YWHAZ RIN1 YWHAZ PARD3 YWHAZ MARK4 YWHAZ PARD6B CFL1 YWHAZ CFL1 MYCBP ING1 PCNA ING1 YWHAZ ING1 RBBP4 ING1 RBP1 OAZ1 OAZ2 LCP2 LYN NSF LUC7L2 BIRC3 CD40 BIRC3 DIABLO EDG3 GNAQ EDG1 PDGFRB RGS3 YWHAZ RGS3 MAPK12 HTRA1 GDF5 SUMO3 VIM CD151 ITGB1 CD151 ITGA3 PER1 TIMELESS ARVCF CDH2 ARVCF TJP1 TGFB1I1 TGFBR2 TGFB1I1 GIT2 PLD1 STXBP1 SERPINI1 PLAT K-ALPHA-1 EPN2 MAP3K3 YWHAZ MAP3K3 WNK1 JAG2 NOTCH1 MFNG NOTCH1 PAK1 TGFBR2 PAK1 ARHGEF6 PAK1 NCK2 PAK1 ABI3 PAK1 SMURF1 PAK1 GIT2 PAK1 PPM1F PAK1 ARPC1B PODXL SLC9A3R2 FHL2 ITGB1 FHL2 IGFBP5 FHL2 ITGB5 FHL2 PSEN2 FHL2 ITGA3 FHL2 WT1 TNFRSF11B TNFSF10 TNFRSF11B TNFSF13 PSMC6 FLJ38984 PSMC6 CCDC85B APBB2 SMURF1 ADAM12 SNX9 NPHS1 NPHS2 NPHS1 NCK2 NPHS1 KIRREL SEPT5 LSM8 PRKAB1 PRKAG1 DNAJA1 TM4SF1 IGFBP7 SDC1 IGFBP7 VEGFA RBBP4 SP3 RBBP4 MBD3 RBBP4 RBP1 SMAD6 SMURF1 SMAD6 TOB1 SGK SLC9A3R2 DHX9 JUN DHX9 PCNA SMARCA4 PB1 SMARCA4 CHMP5 CDK6 CDKN1A CDK6 PCNA MBD3 GATAD2A SNCAIP SH2D3C RNF7 ITM2C EIF3S5 PCID1 ITGA9 ITGB1 ITGA9 SPP1 AKAP9 FNBP1 DLG5 SORBS3 ACTR3 ARPC2 STAT5B TEK STAT5B STAP2 CBLB FYN CBLB NCK2 CBLB SORBS3 ACTN4 ITGB1 ACTN4 GSN ACTN4 SLC9A3R2 ACTN4 PDLIM1 PABPC1 PAIP2 NPHS2 KIRREL GADD45B PCNA GADD45B PPARD GADD45B GADD45G GADD45G LUC7L2 ITGA3 ITGB1 ITGA3 TIMP2 ITGA3 TSPAN4 PRKD1 HDAC7A PRKCH PRKD1 ADAM15 SNX9 PARD3 PARD6B SCARF1 SCARF2 QKI SERF2 QKI C10orf10 QKI RBM9 QKI PCBP4 HSP90AB1 NR2C2 HSP90AB1 MAP3K3 ATF4 JUN ATF4 CEBPB ATF4 POLR2C HHEX JUN RELB SMARCA4 SMURF1 CDGAP UBC UCHL1 UBC YWHAZ UBC OTUD7B UBC DLGAP4 BNIP3L STEAP3 TSC22D1 CORO2B MYCBP CPNE2 ST13 ASF1A GOLPH2 ZFAND3 CHMP6 VPS25 EDNRB NOS3 MCL1 PCNA PBX1 PBX2 PTGDS PTGER2 PRKG1 SPP1 PRKG1 RGS2 PRKG1 MRVI1 CD81 TSPAN4 CD81 DLG5 CD81 IFITM1 UPP1 VIM ID1 IFI16 PCSK5 PTPRM PCSK5 REN INPP5D LYN INPP5D PECAM1 INPP5D JAK3 INPP5D GAB2 HSPB1 TGFB1I1 HSPB1 HSPB8 CLIP2 CLASP2 MPP5 PARD6B PRKD2 HDAC7A MORF4L1 MRFAP1L1 ARL6IP1 SKIP RAB3IL1 RAB11A RIT1 SMURF1 STYX CARHSP1 ITGA8 NPNT ARPC1B ARPC2 ARPC4 ACTR3 ARPC4 ARPC1B ARPC4 ARPC2 SORBS3 DDX17 CALCRL RAMP2 CALCRL RAMP3 TRPC1 TRPC4 GNG11 SMURF1 PLEKHO1 SMURF1 C10orf10 USHBP1 SERTAD3 USHBP1 S100A4 SEPT6 S100A4 TPM2 CCDC85B EXOC8 CCDC85B C21orf91 CCDC85B C10orf10 CCDC85B FAM13C1 GPSM1 USHBP1 LUC7L2 NDUFA4L2 NDRG2 HCCA2 SIAHBP1 SAP30BP USHBP1 FLJ38984 USHBP1 EXOC8 PARD6B PARD3B.
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  • Merlin Is a Potent Inhibitor of Glioma Growth

    Merlin Is a Potent Inhibitor of Glioma Growth

    Research Article Merlin Is a Potent Inhibitor of Glioma Growth Ying-Ka Ingar Lau,1 Lucas B. Murray,1 Sean S. Houshmandi,2 Yin Xu,1 David H. Gutmann,2 and Qin Yu1 1Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York and 2Department of Neurology, Washington University School of Medicine, St. Louis, Missouri Abstract The NF2 gene shares sequence similarity with the members of Neurofibromatosis 2 (NF2) is an inherited cancer syndrome in Band 4.1 superfamily (4, 5). In particular, the NF2 protein, merlin (or schwannomin), most closely resembles proteins of the ezrin- which affected individuals develop nervous system tumors, including schwannomas, meningiomas, and ependymomas. radixin-moesin (ERM) subfamily. Similar to the ERM proteins, The NF2 protein merlin (or schwannomin) is a member of the merlin serves as a linker between transmembrane proteins and the Band 4.1superfamily of proteins, which serve as linkers actin cytoskeleton and regulates cytoskeleton remodeling and cell between transmembrane proteins and the actin cytoskeleton. motility (6–8). Unlike ERM proteins, merlin functions as a negative In addition to mutational inactivation of the NF2 gene in NF2- growth regulator or tumor suppressor. In this regard, mutational associated tumors, mutations and loss of merlin expression inactivation of the NF2 gene is sufficient to result in the have also been reported in other types of cancers. In the development of NF2-associated nervous system tumors. Moreover, present study, we show that merlin expression is dramatically NF2 mutations have also been reported in other tumor types, reduced in human malignant gliomas and that reexpression of including melanoma and mesothelioma (9), suggesting that merlin plays an important role, not only in NF2-associated tumors but also functional merlin dramatically inhibits both subcutaneous and intracranial growth of human glioma cells in mice.
  • Goat Anti-JAM2 / JAMB / CD322 Antibody Peptide-Affinity Purified Goat Antibody Catalog # Af1576a

    Goat Anti-JAM2 / JAMB / CD322 Antibody Peptide-Affinity Purified Goat Antibody Catalog # Af1576a

    10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Goat Anti-JAM2 / JAMB / CD322 Antibody Peptide-affinity purified goat antibody Catalog # AF1576a Specification Goat Anti-JAM2 / JAMB / CD322 Antibody - Product Information Application WB Primary Accession P57087 Other Accession NP_067042, 58494, 67374 (mouse), 619374 (rat) Reactivity Human Predicted Mouse, Rat, Dog, Cow Host Goat Clonality Polyclonal Concentration 100ug/200ul Isotype IgG Calculated MW 33207 Goat Anti-JAM2 / JAMB / CD322 Antibody - AF1576a (0.5 µg/ml) staining of Human Additional Information Heart lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the Gene ID 58494 immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Other Names Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, JAM-2, Goat Anti-JAM2 / JAMB / CD322 Antibody - Vascular endothelial junction-associated Background molecule, VE-JAM, CD322, JAM2, C21orf43, VEJAM Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial Format cell sheets, forming continuous seals around 0.5 mg IgG/ml in Tris saline (20mM Tris cells and serving as a physical barrier to pH7.3, 150mM NaCl), 0.02% sodium azide, prevent solutes and water from passing freely with 0.5% bovine serum albumin through the paracellular space. The protein encoded by this immunoglobulin superfamily Storage gene member is localized in the tight junctions Maintain refrigerated at 2-8°C for up to 6 between high endothelial cells. It acts as an months. For long term storage store at adhesive ligand for interacting with a variety of -20°C in small aliquots to prevent immune cell types and may play a role in freeze-thaw cycles.
  • A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus

    A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus

    Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
  • A Multistep Bioinformatic Approach Detects Putative Regulatory

    A Multistep Bioinformatic Approach Detects Putative Regulatory

    BMC Bioinformatics BioMed Central Research article Open Access A multistep bioinformatic approach detects putative regulatory elements in gene promoters Stefania Bortoluzzi1, Alessandro Coppe1, Andrea Bisognin1, Cinzia Pizzi2 and Gian Antonio Danieli*1 Address: 1Department of Biology, University of Padova – Via Bassi 58/B, 35131, Padova, Italy and 2Department of Information Engineering, University of Padova – Via Gradenigo 6/B, 35131, Padova, Italy Email: Stefania Bortoluzzi - [email protected]; Alessandro Coppe - [email protected]; Andrea Bisognin - [email protected]; Cinzia Pizzi - [email protected]; Gian Antonio Danieli* - [email protected] * Corresponding author Published: 18 May 2005 Received: 12 November 2004 Accepted: 18 May 2005 BMC Bioinformatics 2005, 6:121 doi:10.1186/1471-2105-6-121 This article is available from: http://www.biomedcentral.com/1471-2105/6/121 © 2005 Bortoluzzi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Searching for approximate patterns in large promoter sequences frequently produces an exceedingly high numbers of results. Our aim was to exploit biological knowledge for definition of a sheltered search space and of appropriate search parameters, in order to develop a method for identification of a tractable number of sequence motifs. Results: Novel software (COOP) was developed for extraction of sequence motifs, based on clustering of exact or approximate patterns according to the frequency of their overlapping occurrences.
  • Protein Identities in Evs Isolated from U87-MG GBM Cells As Determined by NG LC-MS/MS

    Protein Identities in Evs Isolated from U87-MG GBM Cells As Determined by NG LC-MS/MS

    Protein identities in EVs isolated from U87-MG GBM cells as determined by NG LC-MS/MS. No. Accession Description Σ Coverage Σ# Proteins Σ# Unique Peptides Σ# Peptides Σ# PSMs # AAs MW [kDa] calc. pI 1 A8MS94 Putative golgin subfamily A member 2-like protein 5 OS=Homo sapiens PE=5 SV=2 - [GG2L5_HUMAN] 100 1 1 7 88 110 12,03704523 5,681152344 2 P60660 Myosin light polypeptide 6 OS=Homo sapiens GN=MYL6 PE=1 SV=2 - [MYL6_HUMAN] 100 3 5 17 173 151 16,91913397 4,652832031 3 Q6ZYL4 General transcription factor IIH subunit 5 OS=Homo sapiens GN=GTF2H5 PE=1 SV=1 - [TF2H5_HUMAN] 98,59 1 1 4 13 71 8,048185945 4,652832031 4 P60709 Actin, cytoplasmic 1 OS=Homo sapiens GN=ACTB PE=1 SV=1 - [ACTB_HUMAN] 97,6 5 5 35 917 375 41,70973209 5,478027344 5 P13489 Ribonuclease inhibitor OS=Homo sapiens GN=RNH1 PE=1 SV=2 - [RINI_HUMAN] 96,75 1 12 37 173 461 49,94108966 4,817871094 6 P09382 Galectin-1 OS=Homo sapiens GN=LGALS1 PE=1 SV=2 - [LEG1_HUMAN] 96,3 1 7 14 283 135 14,70620005 5,503417969 7 P60174 Triosephosphate isomerase OS=Homo sapiens GN=TPI1 PE=1 SV=3 - [TPIS_HUMAN] 95,1 3 16 25 375 286 30,77169764 5,922363281 8 P04406 Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3 - [G3P_HUMAN] 94,63 2 13 31 509 335 36,03039959 8,455566406 9 Q15185 Prostaglandin E synthase 3 OS=Homo sapiens GN=PTGES3 PE=1 SV=1 - [TEBP_HUMAN] 93,13 1 5 12 74 160 18,68541938 4,538574219 10 P09417 Dihydropteridine reductase OS=Homo sapiens GN=QDPR PE=1 SV=2 - [DHPR_HUMAN] 93,03 1 1 17 69 244 25,77302971 7,371582031 11 P01911 HLA class II histocompatibility antigen,
  • To Study Mutant P53 Gain of Function, Various Tumor-Derived P53 Mutants

    To Study Mutant P53 Gain of Function, Various Tumor-Derived P53 Mutants

    Differential effects of mutant TAp63γ on transactivation of p53 and/or p63 responsive genes and their effects on global gene expression. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science By Shama K Khokhar M.Sc., Bilaspur University, 2004 B.Sc., Bhopal University, 2002 2007 1 COPYRIGHT SHAMA K KHOKHAR 2007 2 WRIGHT STATE UNIVERSITY SCHOOL OF GRADUATE STUDIES Date of Defense: 12-03-07 I HEREBY RECOMMEND THAT THE THESIS PREPARED UNDER MY SUPERVISION BY SHAMA KHAN KHOKHAR ENTITLED Differential effects of mutant TAp63γ on transactivation of p53 and/or p63 responsive genes and their effects on global gene expression BE ACCEPTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF Master of Science Madhavi P. Kadakia, Ph.D. Thesis Director Daniel Organisciak , Ph.D. Department Chair Committee on Final Examination Madhavi P. Kadakia, Ph.D. Steven J. Berberich, Ph.D. Michael Leffak, Ph.D. Joseph F. Thomas, Jr., Ph.D. Dean, School of Graduate Studies 3 Abstract Khokhar, Shama K. M.S., Department of Biochemistry and Molecular Biology, Wright State University, 2007 Differential effect of TAp63γ mutants on transactivation of p53 and/or p63 responsive genes and their effects on global gene expression. p63, a member of the p53 gene family, known to play a role in development, has more recently also been implicated in cancer progression. Mice lacking p63 exhibit severe developmental defects such as limb truncations, abnormal skin, and absence of hair follicles, teeth, and mammary glands. Germline missense mutations of p63 have been shown to be responsible for several human developmental syndromes including SHFM, EEC and ADULT syndromes and are associated with anomalies in the development of organs of epithelial origin.