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HISTOLOGY LABORATORY Date: ______Species: ______DEPARTMENT of PATHOLOGY SPECIAL INSTRUCTIONS

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HISTOLOGY LABORATORY Date: ______Species: ______DEPARTMENT of PATHOLOGY SPECIAL INSTRUCTIONS HISTOLOGY LABORATORY Date: __________________ Species: ___________________ DEPARTMENT OF PATHOLOGY SPECIAL INSTRUCTIONS: COLLEGE OF VETERINARY MEDICINE Accession #(s): _______________________________________ UNIVERSITY OF GEORGIA Lab: 706.542.5835 Block #(s): ______________________________________ Fax: 706.542.5828 Pathologist/Requestor: __________________________________ stained@uga .edu LAB USE ONLY: Email and/or Phone: __________________________________ DATE COMPLETED: ______________________ Account Name: ___________________________________ # OF TOTAL SLIDES: ______________________ Account Number: ___________________________________ LIST CONTROLS: _________________________ Large Specimen Histology Account: ___________________________________ TECH: ___________________________________ Custom services are available upon request. Primary antibodies are validated and optimized on canine tissues unless otherwise specified. All IHC requests will be run with validated and optimized protocol. If optimal staining is not achieved there will be a one-time validation fee if further staining is requested. All special stain requests shall be received by 9:00 am for same day service. IHC procedures are run routinely on Tuesday and Friday of each week. No requests will be accepted after 9:00 am on these days. When a change of scheduling is anticipated, notification will be posted or emailed. ROUTINE HISTOLOGY SPECIAL STAINS Frozen Section, H&E Process/Embed/Stain Acid Fast Bacteria (Ziehl-Neelsen) Frozen Section, Unstained Process/Embed Only Acid Fast Bacteria, Modified Frozen Section, Special Stain Recut (1 H&E slide) AgNOR _______________________________ Serial Sections (3 H&E slides) Alcian Blue/PAS Cell Pellet Prep Step Sections (3 H&E slides, 10µm apart) Alcian Blue, pH 1.0 LCM Slide Prep Unstained Plus Slides (+) Alcian Blue, pH 2.5 Surface Decalcification Unstained Probe-On Slides Congo Red, Bennhold’s Bullets (10µm, unstained rolls, 3 per tube) **For full decalcification, use seperate form Copper Deparaffinize Slides PaH100.cv Histology Laboratory Fontana-Masson Coverslip Slides Decalcification Request Form, available in Fraser-Lendrum for fibrin the lab. Decals may also be submitted Giemsa through VetView. Gimenez Gomori stain, reticular fibers Gram stain IMMUNOSTAINS: INFECTIOUS DISEASES Grimelius Aspergillus Eastern Equine Encephalitis Grocott’s Methenamine Silver (GMS) Bartonella henselae EHDV-2 GMS for pythium Hall’s stain for bile Borrelia burgdorferi Feline herpesvirus type I Francisella tularensis Helicobacter pylori, Modified Bovine herpesvirus 1 (IBR) Influenza A Luna stain for eosinophils BRSV Luxol Fast Blue-Cresyl Echt Violet o Monoclonal BVDV Luxol Fast Blue-PASH o Polyclonal Campylobacter spec. Leptospira spec. Jones, Basement Membrane Canine adenovirus type 1 Listeria monocytogenes Masson’s trichrome Canine distemper virus Mayer mucicarmine Neospora caninum Melanin bleach w/ H&E Chlamydia Papillomavirus Melanin bleach w/ Fast Red o Monoclonal Methyl Green-Pyronin Y Parvovirus o Polyclonal Oil Red O Coronavirus Group I o Canine, Feline Okajima o Canine, Feline, Ferret Rabiesvirus Periodic Acid Schiff Coronavirus Group II Sarcocystis neurona o Fungal o Bovine, Elk, Porcine Toxoplasma gondii . Light Green Cryptosporidium parvum . Hematoxylin West Nile virus o Basement Membrane . Light Green IMMUNOSTAINS: TISSUE/CELL MARKERS . Hematoxylin PAS Diastase (w/ & w/out- 2 slides) Calcitonin Chromogranin A MAC 387 PASH/Masson’s trichrome combo Caspase-3 (cleaved) Cox 2 Melan A Perl’s Iron CD11d (canine) Cytokeratin, Pan (Lu-5) Melanoma PNL2 Picosirious Red (Collagen I & III) CD117, c-kit Cytokeratin, Pan (AE1/AE3)2 MHC II (canine) PTAH , muscle striations & fibrin CD18 Desmin Myelin Basic Protein Safranin O for cartilage Canine E-Cadherin Myoglobin Sudan IV (paraffin/lipids) o Toluidine blue Feline Factor VIII related antigen Neurofilament o Verhoeff van Gieson elastic stain GFAP Neuron Specific Enolase o Other:_________ von Kossa calcium stain CD20 Glucagon Oligodendrocyte (OLIG2) Warthin Starry CD21 Hepatocyte Specific Antigen S-100 CD3 IgG Sematostatin CD31 o Species: Smooth Muscle Actin CD45 (canine) _____________ Synaptophysin CD45 RA (canine) Insulin Thyroglobulin Form: PaH039.5 CD79 Ki67 Vimentin Effective: 11/18/14 Lysozyme Wilm’s tumor .
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