HISTOLOGY LABORATORY Date: ______Species: ______DEPARTMENT OF PATHOLOGY SPECIAL INSTRUCTIONS:

COLLEGE OF VETERINARY MEDICINE Accession #(s): ______UNIVERSITY OF GEORGIA Lab: 706.542.5835 Block #(s): ______Fax: 706.542.5828 stained@uga .edu Pathologist/Requestor: ______LAB USE ONLY: Email and/or Phone: ______

DATE COMPLETED: ______Account Name: ______# OF TOTAL SLIDES: ______Account Number: ______LIST CONTROLS: ______ Large Specimen Histology Account: ______TECH: ______

Custom services are available upon request. Primary antibodies are validated and optimized on canine tissues unless otherwise specified. All IHC requests will be run with validated and optimized protocol. If optimal is not achieved there will be a one-time validation fee if further staining is requested. All special stain requests shall be received by 9:00 am

for same day service. IHC procedures are run routinely on Tuesday and Friday of each week. No requests will be accepted after 9:00 am on these days. When a change of scheduling is anticipated, notification will be posted or emailed.

ROUTINE HISTOLOGY SPECIAL STAINS   Frozen Section, H&E Process/Embed/Stain  Acid Fast (Ziehl-Neelsen)   Frozen Section, Unstained Process/Embed Only  Acid Fast Bacteria, Modified   Frozen Section, Special Stain Recut (1 H&E slide)  AgNOR  ______Serial Sections (3 H&E slides)  Alcian Blue/PAS   Cell Pellet Prep Step Sections (3 H&E slides, 10µm apart)  Alcian Blue, pH 1.0   LCM Slide Prep Unstained Plus Slides (+)  Alcian Blue, pH 2.5   Surface Decalcification Unstained Probe-On Slides  , Bennhold’s  Bullets (10µm, unstained rolls, 3 per tube)  **For full decalcification, use seperate form Copper  Deparaffinize Slides  PaH100.cv Histology Laboratory Fontana-Masson  Coverslip Slides  Decalcification Request Form, available in Fraser-Lendrum for fibrin  the lab. Decals may also be submitted Giemsa  through VetView. Gimenez  Gomori stain, reticular fibers  IMMUNOSTAINS: INFECTIOUS DISEASES  Grimelius  Aspergillus  Eastern Equine Encephalitis  Grocott’s Methenamine Silver (GMS)

 Bartonella henselae  EHDV-2  GMS for pythium  Hall’s stain for bile  Borrelia burgdorferi  Feline herpesvirus type I  Francisella tularensis  Helicobacter pylori, Modified  Bovine herpesvirus 1 (IBR)  Influenza A  Luna stain for eosinophils  BRSV  Luxol Fast Blue-Cresyl Echt Violet o Monoclonal  BVDV  Luxol Fast Blue-PASH o Polyclonal  Campylobacter spec.  Leptospira spec.  Jones, Basement Membrane  Canine adenovirus type 1  Listeria monocytogenes  Masson’s trichrome   Canine distemper virus Mayer mucicarmine    Neospora caninum Melanin bleach w/ H&E Chlamydia   Papillomavirus Melanin bleach w/ Fast Red o Monoclonal  Methyl Green-Pyronin Y  Parvovirus o Polyclonal  Red O  Coronavirus Group I o Canine, Feline  Okajima  o Canine, Feline, Ferret Rabiesvirus  Periodic Acid Schiff  Coronavirus Group II  Sarcocystis neurona o Fungal o Bovine, Elk, Porcine  Toxoplasma gondii . Light Green  Cryptosporidium parvum . Hematoxylin  West Nile virus o Basement Membrane . Light Green IMMUNOSTAINS: TISSUE/CELL MARKERS . Hematoxylin  PAS Diastase (w/ & w/out- 2 slides)  Calcitonin  Chromogranin A  MAC 387  PASH/Masson’s trichrome combo    Caspase-3 (cleaved) Cox 2 Melan A  Perl’s Iron  CD11d (canine)  Cytokeratin, Pan (Lu-5)  Melanoma PNL2  Picosirious Red (Collagen I & III)  CD117, c-kit  Cytokeratin, Pan (AE1/AE3)2  MHC II (canine)  PTAH , muscle striations & fibrin  CD18  Desmin  Myelin Basic Protein  O for cartilage Canine  E-Cadherin  Myoglobin  Sudan IV (paraffin/) o  Toluidine blue Feline  Factor VIII related antigen  Neurofilament o  Verhoeff van Gieson elastic stain  GFAP  Neuron Specific Enolase o Other:______ von Kossa calcium stain  CD20  Glucagon  Oligodendrocyte (OLIG2)  Warthin Starry  CD21  Hepatocyte Specific Antigen  S-100  CD3  IgG  Sematostatin  CD31 o Species:  Smooth Muscle Actin  CD45 (canine) ______ Synaptophysin  CD45 RA (canine)  Insulin  Thyroglobulin Form: PaH039.5  CD79  Ki67  Vimentin Effective: 11/18/14  Lysozyme  Wilm’s tumor