DOCSLIB.ORG

Sudan IV Stain

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Sudan IV Stain [+1] 800.221.6058 United States Directions For Use for the following: [+1] 360.260.2779 International Sudan IV Stain INTENDED USE BIBLIOGRAPHY Sudan IV Stain is used to detect fat in feces, urine and tissues. Patients 1. Balows, A., et al., 1991. Manual of Clinical Microbiology. ASM, demonstrating fat in stool (i.e., steatorrhea) may have a correlation to Washington, D.C., Fifth Ed. pancreatic diseases or other fat absorption diseases. 2. Clark, G. Staining Procedures, 1973, Biological Stain Commission. 3. Drummey, G.D.F., et al., 1961. “Microscopically Examination of the SUMMARY Stool for Steatorrhea,” Medical Intelligence”. Vol. 264, pp. 85-87. Intracellular protein-bound lipids are readily demonstrable by the use of 4. Gradwohl’s Clinical Laboratory Methods and Diagnosis, Eighth Sudan dyes when dissolved (or mixed) in a lipid solvent or wet Edition, 1980. specimen. When the specimen is mixed with the dye solvent (stain) the 5. Sonnenwirth, A., et al. 1980 Gradwohl’s Clinical Laboratory dye leaves the solvent for the intracellular (or extracellular) lipid, which Methods and Diagnosis, C.V. Mosby Co. takes on the color of the dye. The staining process is a physical 6. Van de Kamer, J.H., ten Bokkel Huinick, H., and Weyers, H.A. 1949 phenomenon that does not differentiate between the various types of “Rapid method for Determination of Fat in Feces”. J. Biology lipids. Sudan III and Sudan IV will stain neutral fats, while Sudan B will Chem., 177:347-355, 1949. identify phospholipids. CONTACT FOR IN VITRO DIAGNOSTIC USE ONLY Alpha-Tec Systems, Inc. offers a complete line of reagents, stains, and QC1™ Quality Control Slides for AFB, Parasitology, Bacteriology, and PRECAUTIONS Mycology processing, as well as O&P collection systems and For safety considerations, analysis of samples and cultures should be concentration devices for Parasitology. For Technical Assistance, email performed within a certified Class II biological safety cabinet (BSC). Any [email protected], and for Customer Service, email laboratory procedure involving infectious materials or cultures requires [email protected], or call either [+1] 800.221.6058 (USA) standard personal protective safety equipment, such as latex gloves and or [+1] 360.260.2779 between 8AM and 4PM Monday through Friday, laboratory coats (or disposable coats), and eye protection. Pacific Time. Decontamination can be accomplished with commercially available bleach solutions, diluted to an appropriate working concentration. WARRANTY Countertops, work surfaces and instruments can be wiped with the This product is warranted by Alpha-Tec Systems, Inc. to perform as bleach solution. Lab supplies (pipettes, loops, microscope slide, etc.) described in the labeling and literature supplied. Alpha-Tec Systems, should be immersed in decontamination solution and then autoclaved. Inc. disclaims any implied warranty or merchantability or fitness for any other purpose, and in no event shall Alpha-Tec Systems, Inc. be liable STABILITY AND STORAGE for any consequential damages arising out of aforesaid express warranty. The stain solution is stable for 18 months following manufacturing. Refer to the listed expiration date on the stain reagent bottles. Store the stain TRADEMARKS solution at 15-30ºC (room temperatures) away from direct light. QC1™ is a trademark of Alpha-Tec Systems, Inc., 1311 SE Cardinal Court, Suite 170, Vancouver, WA 98683 USA. USER QUALITY CONTROL Positive Control: Use a known source of neutral fats. PRODUCT CODES Out of Control Results: Recheck the positive control as a source of 0003538 Sudan IV Stain, 25 ml neutral fat. Repeat the stain test. If the Positive Control fails, reorder a new lot of stain. SPECIMEN COLLECTION AND PROCESSING The most common specimens utilized for the Sudan IV Stain is feces, urine or tissue. For patients suspected of steatorrhea, a fecal specimen is indicated. PROCEDURE Materials Provided: Sudan IV Stain Materials Not Provided: Applicator sticks, slides, coverslips, immersion oil, microscope SPECIMEN PROCESSING 1. Place a small portion of the sample onto a clean glass slide. 2. Add a few drops of the Sudan IV Stain to the sample and mix into the sample with an applicator stick. 3. Coverslip the stained specimen. If it becomes necessary to extend the viewing time, seal the coverslip to avoid evaporation of the dye. 4. View under a light microscope with low and high power. EXPECTED RESULTS Fat will stain orange to red. Cholesterol will stain less brilliant red. Cellular fatty acids will not stain. NOTE: Sudan III will give a darker orange-red or red color and is more stable in alcohol than Sudan IV. L003538.D // CC19-020, Effective Date: 16 May 2019 // ©2019 Alpha-Tec Systems, Inc. All rights reserved. 1 [+1] 800.221.6058 United States Directions For Use for the following: [+1] 360.260.2779 International Sudan IV Stain L003538.D // CC19-020, Effective Date: 16 May 2019 // ©2019 Alpha-Tec Systems, Inc. All rights reserved. 2 .
Load more

Recommended publications
  • The Morphology, Androgenic Function, Hyperplasia, and Tumors of the Human Ovarian Hilus Cells * William H
    THE MORPHOLOGY, ANDROGENIC FUNCTION, HYPERPLASIA, AND TUMORS OF THE HUMAN OVARIAN HILUS CELLS * WILLIAM H. STERNBERG, M.D. (From the Department of Pathology, School of Medicine, Tulane University of Louisiana and the Charity Hospital of Louisiana, New Orleans, La.) The hilus of the human ovary contains nests of cells morphologically identical with testicular Leydig cells, and which, in all probability, pro- duce androgens. Multiple sections through the ovarian hilus and meso- varium will reveal these small nests microscopically in at least 8o per cent of adult ovaries; probably in all adult ovaries if sufficient sections are made. Although they had been noted previously by a number of authors (Aichel,l Bucura,2 and von Winiwarter 3"4) who failed to recog- nize their significance, Berger,5-9 in 1922 and in subsequent years, pre- sented the first sound morphologic studies of the ovarian hilus cells. Nevertheless, there is comparatively little reference to these cells in the American medical literature, and they are not mentioned in stand- ard textbooks of histology, gynecologic pathology, nor in monographs on ovarian tumors (with the exception of Selye's recent "Atlas of Ovarian Tumors"10). The hilus cells are found in clusters along the length of the ovarian hilus and in the adjacent mesovarium. They are, almost without excep- tion, found in contiguity with the nonmyelinated nerves of the hilus, often in intimate relationship to the abundant vascular and lymphatic spaces in this area. Cytologically, a point for point correspondence with the testicular Leydig cells can be established in terms of nuclear and cyto- plasmic detail, lipids, lipochrome pigment, and crystalloids of Reinke.
    [Show full text]
  • Lysochrome Dyes Sudan Dyes, Oil Red Fat Soluble Dyes Used for Biochemical Staining of Triglycerides, Fatty Acids, and Lipoproteins Product Description
    FT-N13862 Lysochrome dyes Sudan dyes, Oil red Fat soluble dyes used for biochemical staining of triglycerides, fatty acids, and lipoproteins Product Description Name : Sudan IV Other names: Sudan R, C.I. Solvent Red 24, C.I. 26105, Lipid Crimson, Oil Red, Oil Red BB, Fat Red B, Oil Red IV, Scarlet Red, Scarlet Red N.F, Scarlet Red Scharlach, Scarlet R Catalog Number : N13862, 100g Structure : CAS: [85-83-6] Molecular Weight : MW: 380.45 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =513-529nm(red);Sol(EtOH):0.09% S:22/23/24/25 Name : Sudan III Other names: Rouge Sudan ; rouge Ceresin ; CI 26100; CI Solvent Red 23 Catalog Number : 08002A, 25g Structure : CAS:[85-86-9] Molecular Weight : MW: 352.40 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =503-510nm(red);Sol(EtOH):0.15% S:24/25 Name : Sudan Black B Other names: Sudan Black; Fat Black HB; Solvent Black 3; C.I. 26150 Catalog Number : 279042, 50g AR7910, 100tests stain for lipids granules Structure : CAS: [4197-25-5] S:22/23/24/25 Molecular Weight : MW: 456.54 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs=596-605nm(blue-black) Name : Oil Red O Other names: Solvent Red 27, Sudan Red 5B, C.I. 26125 Catalog Number : N13002, 100g Structure : CAS: [1320-06-5 ] Molecular Weight : MW: 408.51 λabs = 513-529 nm (red); Sol(EtOH): 0.09%abs =518(359)nm(red);Sol(EtOH): moderate; Sol(water): Insoluble S:22/23/24/25 Storage: Room temperature (Z) P.1 FT-N13862 Technical information & Directions for use A lysochrome is a fat soluble dye that have high affinity to fats, therefore are used for biochemical staining of triglycerides, fatty acids, and lipoproteins.
    [Show full text]
  • An Improved Method of Staining Lipides : - Acetic- Carbol - Sudan
    . Onderstepoort J ournal of Vetet·inary Science and Animal l ndustrJJ, Volu-me 19, Nmnbers 1 and 2, Jamwry and April, 1944. Printed in the Union of South Africa by the Government Printer, Pretoria. An Improved Method of Staining Lipides : - Acetic- Carbol - Sudan. By CECIL JACKSON, Section of Anatomy, Onderstepoort. INTRODUCTION. THE literature on the microscopical demonstration of fats and fat-like substances is largely a record of dissatisfaction with previous methods. It is noteworthy how many authors, investigating the lipides of normal or patho­ logical tissues, have felt impelled to turn their attention to imprpvement of technique. Some of these have gone_ further and have become absorbed in the problem of fat-staining for its own sake and apart from the difficulties they originally encountered. 'l'he technique of Sudan staining is essentially a physico-chemical prqblem and histologist§ or pathologists venturing into this realm have sometimes had to run the gauntlet of expert criticism. This danger may act as a deterrent to investigation; but it is comforting to reflect that a successful technique will stand up to a lot of argument about its rationale: a good method will remain, irrespective of the ability of its author to defend or explain the theory underlying it, Many attempts have been made, either to modify the methods of using existing dyes or to introduce new dyes in the hope of securing improvement in fat staining. Thus we have seen " Sudan III ", " Scharlach R ", and " Sudan IV " successively become _the favourite or at least the most highly recommended substances; and there are a number of technical variations depending on the solvents used or on the -procedure of making the dilutions from stock solutions.
    [Show full text]
  • How to Construct and Use a Simple Device to Prevent the Formation of Precipitates When Using Sudan Black B for Histology
    Acta Botanica Brasilica 29(4): 489-498. 2015. doi: 10.1590/0102-33062015abb0093 How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology João Marcelo Santos de Oliveira1 Received: April 17, 2015. Accepted: July 1, 2015 ABSTRACT The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixa- tion of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of precipitates. Furthermore, by placing the sections facing downward the device, any sporadically formed precipitates are prevented from settling on and fixing to the sectioned tissues or organs. By avoiding the formation of precipitates, plant cells, tissues and organs can be better observed, diagnosed and photomicrographically recorded. Keywords: histochemical tests, histology, lipids, plant anatomy, Sudan Black B Introduction for organic solvents, printer ink, varnishes, resins, oils, fats, waxes, cosmetics and contact lenses. Sudan reagents, including the traditional Sudan III, IV Lansink (1968) isolated two pure fractions of Sudan and Sudan Black B (SBB), are widely utilized to determine Black B, in addition to impurities, and denominated them lipids (Horobin 2002) in animals, plants and hydrophobic SBB-I and SBB-II.
    [Show full text]
  • A Method for Staining Whole Brains for Gross and Macroscopic Study by W
    [ 134 ] A METHOD FOR STAINING WHOLE BRAINS FOR GROSS AND MACROSCOPIC STUDY BY W. HEWITT Department of Anatomy, St Thomas's Hospital Medical School, London A number of staining techniques are available for the macroscopic examination of thick slices of brain. Examples are those of Mulligan (1931) or Tompsett (1955), which stain the grey matter; or those of Waldman & Michaels (1954) or Brody & Wirth (1957), which colour the white matter with sudan stains. In all of these only the surface of a previously cut slice is stained and surface staining has a number of disadvantages. The techniques of Mulligan (1931) and Tompsett (1955) require a painstaking removal of the membranes and blood vessels, before sections are cut, to prevent smearing of the grey and white matter. Even then success is by no means always assured. Surface-stained brain slices will also be spoilt, by exposure ofunstained areas underneath, if accidentally knocked or chipped. Moreover, the section originally made may not be the one desired and it is not possible to pare away the surface to the desired level. Apart from these disadvantages, it can be useful to stain a whole brain so that when it is sectioned the grey and white matter can be clearly distinguished. For these reasons a technique has been developed for staining a whole brain using sudan stains. This was devised before the writer was aware of the staining methods of Waldman & Michaels (1954) and Brody & Wirth (1957); it is in fact based on a different principle. METHOD The brains can be fixed in either 70 % alcohol or, for preference, 10 % formaldehyde.
    [Show full text]
  • Sudan Dyes and Other Illegal Dyes
    Sudan dyes and other illegal dyes Analysis in spices and food Sudan dyes, e.g. Sudan I to IV, Sudan In October 2004, the governmental Orange G, Sudan Red B, Sudan Red G, chemical institute “Bergisches Land” in Sudan Red 7B, and Sudan Black B as Wuppertal, Germany, reported the well as other dyes like 4-(Dimethylamino)- identification of two prohibited colours: azobenzene (Butter Yellow) and Para Butter Yellow and Para Red in bell pepper Red are basically synthetically produced powder and curry. azo dyes. Their degradation products are In February 2005, Great Britain experi- considered to be carcinogens and enced an extensive Rapid Alert action teratogens. Due to this fact, the EU does cycle. Due to the widespread use of one not permit the use of these colours as batch of chilli powder contaminated with food additives. However, in some Sudan I, batches of different products like countries, these dyes are still occasionally Worcester sauce, pizzas, pot noodles and used in order to intensify the colour of bell seafood sauces were subjected to com- pepper and chilli powder. plete recalls. Background Another recent concern is the discovery of the natural colour annatto in food and During 2003, the EU-Rapid Alert System spice mixes. Annatto (E 160b) is permit- (RASFF) issued a series of notifications ted by the EU for use in a variety of foods concerning the presence of Sudan dyes and beverages but not in spices and in chilli products and others such as spice mixtures. Its main colouring constit- spices, mixtures of spices, tomato uent is Bixin, with Norbixin being present sauces, pastas and sausages.
    [Show full text]
  • Application of Standard Micro-Anatomical Staining Methods to Epoxy Resin-Embedded Sections
    J Clin Pathol: first published as 10.1136/jcp.22.5.589 on 1 September 1969. Downloaded from J. clin. Path. (1969), 22, 589-592 Application of standard micro-anatomical staining methods to epoxy resin-embedded sections S. R. APARICIO AND P. MARSDEN From the Department ofPathology, School of Medicine, Leeds SYNOPSIS Staining of semi-thin sections of osmium-fixed epoxy resin (Epon or Araldite)-embedded tissues with haematoxylin and acid dyes as counterstains (eosin or phloxin) was successfully achieved after intense oxidation using hydrogen peroxide as the oxygen donor. Results are closely similar to those obtained with paraffin sections (haematoxylin and eosin): nuclei stain blue, cytoplasm and extracellular structures (eg, collagen, elastica, basement membranes) stain with varying shades of red. Preliminary studies show that similarly good results, comparable with standard paraffin- embedded histological materials, can be obtained, using H202 pretreatment, with Weigert's iron haematoxylin, Mallory's phosphotungstic acid haematoxylin, Alcian blue, PAS reaction, Sudan III, Sudan black, oil red, Gomori's aldehyde fuchsin, and Baker's acid haematein. Staining of semi-thin sections of osmium-fixed, since Epon and Araldite became universally adopted copyright. epoxy resin-embedded tissues was developed in as embedding media. the last decade, mainly using basic aniline dyes This communication reports a simple and efficient in neutral or alkaline solutions (Bencosme, Stone, method of obtaining haematoxylin-eosin and haema- Latta, and Madden, 1959; Moore, Mumaw, and toxylin-phloxin epoxy resin preparations identical Schoenberg, 1960; Richardson, Jarett, and Finke, in colour contrast with paraffin sections. 1960; Trump, Smuckler, and Benditt, 1961; Grimley, Albrecht, and Michelitch, 1965; Lee and Hopper, MATERIAL AND METHODS 1965; Aoki and Gutierrez, 1967; Aparicio and Mars- http://jcp.bmj.com/ den, 1969; Huber, Parker, and Odland, A variety of tissues (human brain, peripheral nerve, 1968).
    [Show full text]
  • Histochemical Studies on Two Milliped Species1-2
    HISTOCHEMICAL STUDIES ON TWO MILLIPED SPECIES1-2 RAYMOND C. BOWEN Department of Biology, The Cleveland State University, Cleveland, Ohio ABSTRACT Histological and histochemical tests give similar results for the midguts of Floridobolus penneri (Causey, 1957) and Narceus gordanus (Chamberlin, 1943). The peritrophic mem- brane is composed of basic proteins and acid mucopolysaccharides. The epithelium contains basic proteins and large lipid concentrations, including glycolipids, phospholipids, and fatty acids. The luminal epithelial border gives reactions for protein-bound amino groups, tyrosine and phenolic compounds, and neutral fats. Protein-bound amino groups, glycoproteins, acid mucopolysaccharides, and bound lipids are found in the collagenous basement membrane. The circular and longitudinal muscle layers contain basic proteins, tyrosine and phenols, and bound fats. A large glycogen concentration occurs within the sheath membrane. This region, which is primarily basic protein, also gives positive reactions for protein-bound groups and both tyrosine and phenols. Little work has been done on the internal anatomy of diplopods. Early anatomical studies included investigations by Verhoeff (1914), Randow (1924), Attems (1926), Hefner (1929), and Miley (1930). Most biochemical analyses have included the whole animal and not particular organs or tissues. Siddiqui, et al. (1944) isolated dimethylglyoxime from a species of Indian diplopod. Bergmann (1949) reported that Ueno and Yamasaki had isolated a sterol from the saponifiable matter of millipeds.
    [Show full text]
  • STAINING TECHNIQUES — Staining Is an Auxiliary Technique Used in Microscopy to Enhance Contrast in the Microscopic Image
    STAINING TECHNIQUES Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains or dyes are used in biology and medicine to highlight structures in biological tissues for viewing with microscope. Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. Using different stains, it is possible to stain preferentially certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living or non-living cells. In biochemistry, staining involves adding a class specific (DNA, lipids, proteins or carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescence tagging can serve similar purposes Purposes of Staining The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample. Cells may also be enumerated by staining cells to determine biomass in an environment of interest. Stains may be used to define and examine bulk tissues (e.g. muscle fibers or connective tissues), cell populations (different blood cells) or organelles within individual cells. Biological staining is also used to mark cells in flow cytometry, flag proteins or nucleic acids on gel electrophoresis Staining is not limited to biological materials, it can also be used to study the morphology (form) of other materials e.g.
    [Show full text]
  • Chapter 9 a Beginner's Guide to the Study of Plant Structure
    Chapter 9 A Beginner's Guide to the Study of Plant Structure Edward C. Yeung Department of Biological Sciences University of Calgary Calgary, Alberta, Canada T2N 1N4 Tel: (403) 220-7186; e-mail: [email protected] Edward C. Yeung obtained his B.Sc. from the University of Guelph in 1972 and a Ph.D. in biology from Yale University in 1977. After spending one year as a postdoctoral fellow at the University of Ottawa, Dr. Yeung joined the Department of Biological Sciences, University of Calgary, where he is now a Professor. His primary research interests have been reproductive biology of higher plants, especially the structural and physiological aspects of embryo development. Reprinted From: Yeung, E. 1998. A beginner’s guide to the study of plant structure. Pages 125-142, in Tested studies for laboratory teaching, Volume 19 (S. J. Karcher, Editor). Proceedings of the 19th Workshop/Conference of the Association for Biology Laboratory Education (ABLE), 365 pages. - Copyright policy: http://www.zoo.utoronto.ca/able/volumes/copyright.htm Although the laboratory exercises in ABLE proceedings volumes have been tested and due consideration has been given to safety, individuals performing these exercises must assume all responsibility for risk. The Association for Biology Laboratory Education (ABLE) disclaims any liability with regards to safety in connection with the use of the exercises in its proceedings volumes. © 1998 Edward C. Yeung Association for Biology Laboratory Education (ABLE) ~ http://www.zoo.utoronto.ca/able 125 126 Botanical Microtechniques
    [Show full text]
  • Permanent Slides of Plant Cuticle Stained with Sudan IV and Sudan Black B
    Proceedings of the Iowa Academy of Science Volume 49 Annual Issue Article 14 1942 Permanent Slides of Plant Cuticle Stained with Sudan IV and Sudan Black B H. L. Dean State University of Iowa Edwards Sybil Jr. State University of Iowa Let us know how access to this document benefits ouy Copyright ©1942 Iowa Academy of Science, Inc. Follow this and additional works at: https://scholarworks.uni.edu/pias Recommended Citation Dean, H. L. and Sybil, Edwards Jr. (1942) "Permanent Slides of Plant Cuticle Stained with Sudan IV and Sudan Black B," Proceedings of the Iowa Academy of Science, 49(1), 129-132. Available at: https://scholarworks.uni.edu/pias/vol49/iss1/14 This Research is brought to you for free and open access by the Iowa Academy of Science at UNI ScholarWorks. It has been accepted for inclusion in Proceedings of the Iowa Academy of Science by an authorized editor of UNI ScholarWorks. For more information, please contact [email protected]. Dean and Sybil: Permanent Slides of Plant Cuticle Stained with Sudan IV and Sudan PERMANENT SLIDES OF PLANT CUTICLE STAINED WITH SUDAN IV AND SUDAN BLACK B H. L. DEAN AND EDWARD SvmL, JR. Sudan IV is commonly used to stain fats, oils, suberin, and cut­ in. Materials stained in this dye are usually mounted temporarily in glycerine and are seldom kept as permanent slides. This may be due to the fact that balsam, clarite or similar mounting media, cannot be used to make permanent slides of preparations stained in Sudan IV. The dye is immediately removed by the xylene or toulene solvent of these media, leaving the preparations colorless.
    [Show full text]
  • Sudan IV (S8756)
    Sudan IV Product Number S 8756 Store at Room Temperature Product Description Preparation Instructions Molecular Formula: C24H20N4O This dye is soluble in chloroform (1 mg/ml). It is Molecular Weight: 380.5 soluble in water (0.7 mg/ml), slightly soluble in ethanol CAS Number: 85-83-6 and very soluble in benzene, methanol, acetone, 1 1 λmax: 520 nm, 357 nm (toluene) hydrocarbon solvents, oils, fats, and waxes. Synonyms: Oil Red IV, Fat ponceau, Cerotin ponceau 2 3B Storage/Stability Procedures using either isopropanol or ethanol as 3 This red β-naphthol disazo dye has the same basic stock solutions have been reported. The isopropanol structure as Sudan III with two added methyl groups. stock solution is diluted in water (6 ml of stock : 4 ml of This makes it a deeper, more intense stain, but with water), allowed to stand 5-10 minutes, and then the hydroxyl group in the ortho position, it has similar filtered. The filtrate can be used for several hours. physical properties and is fat soluble.1,2 This dye has Another stock solution is 0.1 gram Sudan IV in a been used industrially to color oils, waxes, shoe mixture of 50 ml 70% alcohol and 50 ml acetone. This polish, greases, oily-resin lacquers, varnishes, solution should be made fresh every few days. cellulose acetate and acrylic resins, and in wood stains. As a biological stain, it is used primarily to References stain lipids and fatty substances present in cells and 1. The Sigma-Aldrich Handbook of Stains, Dyes & tissues. The color imparted is a function of the Indicators, Green, F.J., ed., Aldrich Chemical Co.
    [Show full text]