Supporting Information

Lundqvist et al. 10.1073/pnas.0805135105

Fig. S1. The different pellet sizes with different plasma concentrations, reflected as the amount of protein detected with SDS/PAGE. The numbers refer to the different polystyrene particles: 1, 100-nm amine-modified; 2, 50-nm amine-modified; 3, 100-nm plain; 4, 50-nm plain; 5, 100-nm carboxyl-modified; and 6, 50-nm carboxyl-modified. The letters refer to different plasma concentrations: a, 0.056; b, 0.28; c, 0.56; d, 2.8; and e, 5.6 ml plasma per m2 particle surface.

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 1of9 Fig. S2. The gel shows how the different pellet sizes are reflected in the intensity of the bands in a Coomassie blue-stained gel. The letters refer to different plasma concentrations: a, 0.056; b, 0.28; c, 0.56; d, 2.8; e, 5.6; and f, 7.8 ml plasma per m2 particle surface.

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 2of9 Fig. S3. SDS/PAGE (12%) of plasma proteins eluted from negatively and positively charged NIPAM/BAM copolymer particles (50:50 ratio of comonomers, size Ϸ50 nm) after washing.

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 3of9 Table S2. Proteins identified on each particle 100-nm amine-modified

P02647 Apolipoprotein A-I P04114 Apolipoprotein B-100 P02654 Apolipoprotein C-I P02649 Apolipoprotein E P02749 Beta-2-glycoprotein 1 Q96IY4 Carboxypeptidase B2 P10909 Clusterin P12259 Coagulation factor V P03951 Coagulation factor XI P02746 Complement C1q subcomponent subunit B P02747 Complement C1q subcomponent subunit C P08603 Complement factor H Q03591 Complement factor H-related protein 1 Q02985 Complement factor H-related protein 3 P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain Q04756 Hepatocyte growth factor activator P04196 Histidine-rich glycoprotein Q14520 Hyaluronan-binding protein 2 P17936 Insulin-like growth factor-binding protein 3 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 P01042 Kininogen-1 P18428 Lipopolysaccharide-binding protein P03952 Plasma kallikrein P05155 Plasma protease C1 inhibitor P05154 Plasma serine protease inhibitor P00747 Plasminogen P02768 Serum albumin P04004 Vitronectin P01857 Ig gamma-1 chain C region P01859 Ig gamma-2 chain C region P01861 Ig gamma-4 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions P01871 Ig mu chain C region

50-nm amine-modified P63104 14–3–3 protein zeta/delta P01011 Alpha-1-antichymotrypsin P01009 Alpha-1-antitrypsin P08697 Alpha-2-antiplasmin P02760 AMBP protein P02647 Apolipoprotein A-I P02652 Apolipoprotein A-II P06727 Apolipoprotein A-IV P04114 Apolipoprotein B-100 P02654 Apolipoprotein C-I P02656 Apolipoprotein C-III P05090 Apolipoprotein D P02649 Apolipoprotein E Q13790 Apolipoprotein F O14791 Apolipoprotein-L1 Q96KN2 Beta-Ala-His dipeptidase P04003 C4b-binding protein alpha chain O43866 CD5 antigen-like P00450 Ceruloplasmin P10909 Clusterin P00736 Complement C1r subcomponent P09871 Complement C1 s subcomponent P08603 Complement factor H P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 4of9 100-nm amine-modified

P02751 Fibronectin P00738 Haptoglobin P00739 Haptoglobin-related protein P19827 Inter-alpha-trypsin inhibitor heavy chain H1 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 Q06033 Inter-alpha-trypsin inhibitor heavy chain H3 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 O00187 Mannan-binding lectin serine protease 2 P11226 Mannose-binding protein C P04180 Phosphatidylcholine-sterol acyltransferase P80108 Phosphatidylinositol-glycan-specific phospholipase D P05155 Plasma protease C1 inhibitor P02768 Serum albumin P27169 Serum paraoxonase/arylesterase 1 Q15166 Serum paraoxonase/lactonase 3 P05452 Tetranectin P02766 Transthyretin P07225 Vitamin K-dependent protein S P04004 Vitronectin P01876 Ig alpha-1 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions P01871 Ig mu chain C region

100-nm plain P01011 Alpha-1-antichymotrypsin P01009 Alpha-1-antitrypsin P02765 Alpha-2-HS-glycoprotein P02760 AMBP protein P01008 Antithrombin-III P02647 Apolipoprotein A-I P06727 Apolipoprotein A-IV P04114 Apolipoprotein B-100 P05090 Apolipoprotein D P02649 Apolipoprotein E P02749 Beta-2-glycoprotein 1 P04003 C4b-binding protein alpha chain O43866 CD5 antigen-like P00450 Ceruloplasmin P10909 Clusterin P02746 Complement C1q subcomponent subunit B P02747 Complement C1q subcomponent subunit C P09871 Complement C1 s subcomponent P01024 Complement C3 P0C0L4 Complement C4-A P0C0L5 Complement C4-B P01031 Complement C5 P07357 Complement component C8 alpha chain P02748 Complement component C9 P00751 Complement factor B P08603 Complement factor H P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain P02751 Fibronectin P06396 Gelsolin P00738 Haptoglobin P00739 Haptoglobin-related protein P02790 Hemopexin P19827 Inter-alpha-trypsin inhibitor heavy chain H1 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 Q06033 Inter-alpha-trypsin inhibitor heavy chain H3 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 P01042 Kininogen-1 P02753 Plasma retinol-binding protein

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 5of9 100-nm amine-modified

P00747 Plasminogen P02787 Serotransferrin P02768 Serum albumin P02743 Serum amyloid P-component P27169 Serum paraoxonase/arylesterase 1 Q6PJ69 Tripartite motif-containing protein 65 P02774 Vitamin D-binding protein P07225 Vitamin K-dependent protein S P04004 Vitronectin P01876 Ig alpha-1 chain C region P01877 Ig alpha-2 chain C region P01857 Ig gamma-1 chain C region P01859 Ig gamma-2 chain C region P01860 Ig gamma-3 chain C region P01861 Ig gamma-4 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions P01871 Ig mu chain C region Ig kappa chain V-I region Ig kappa chain V-II region Ig heavy chain V-III region Ig kappa chain V-IV region

50-nm plain P01011 Alpha-1-antichymotrypsin P02765 Alpha-2-HS-glycoprotein P02760 AMBP protein P02647 Apolipoprotein A-I P06727 Apolipoprotein A-IV P02656 Apolipoprotein C-III P05090 Apolipoprotein D P02749 Beta-2-glycoprotein 1 P04003 C4b-binding protein alpha chain P10909 Clusterin P02746 Complement C1q subcomponent subunit B P02747 Complement C1q subcomponent subunit C P09871 Complement C1 s subcomponent P01024 Complement C3 P0C0L4 Complement C4-A P0C0L5 Complement C4-B P01031 Complement C5 P13671 Complement component C6 P07357 Complement component C8 alpha chain P00751 Complement factor B P08603 Complement factor H P36980 Complement factor H-related protein 2 Q02985 Complement factor H-related protein 3 P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain P02751 Fibronectin P06396 Gelsolin P00738 Haptoglobin P00739 Haptoglobin-related protein P68871 Hemoglobin subunit beta P02790 Hemopexin P04196 Histidine-rich glycoprotein P19827 Inter-alpha-trypsin inhibitor heavy chain H1 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 P01042 Kininogen-1 P51884 Lumican P02753 Plasma retinol-binding protein P00747 Plasminogen P02787 Serotransferrin

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 6of9 100-nm amine-modified

P02768 Serum albumin P35542 Serum amyloid A-4 protein P27169 Serum paraoxonase/arylesterase 1 P05452 Tetranectin P02766 Transthyretin P02774 Vitamin D-binding protein P07225 Vitamin K-dependent protein S P04004 Vitronectin P01876 Ig alpha-1 chain C region P01857 Ig gamma-1 chain C region P01859 Ig gamma-2 chain C region P01860 Ig gamma-3 chain C region P01861 Ig gamma-4 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions P01871 Ig mu chain C region P01591 Immunoglobulin J chain Ig kappa chain V-I region Ig kappa chain V-II region Ig heavy chain V-III region Ig kappa chain V-IV region

100-nm carboxyl-modified P01011 Alpha-1-antichymotrypsin P01009 Alpha-1-antitrypsin P08697 Alpha-2-antiplasmin P02760 AMBP protein P02647 Apolipoprotein A-I P06727 Apolipoprotein A-IV P04114 Apolipoprotein B-100 P02654 Apolipoprotein C-I O14791 Apolipoprotein-L1 P02749 Beta-2-glycoprotein 1 P04003 C4b-binding protein alpha chain P10909 Clusterin P02746 Complement C1q subcomponent subunit B P02747 Complement C1q subcomponent subunit C P01024 Complement C3 P0C0L4 Complement C4-A P0C0L5 Complement C4-B P00751 Complement factor B P08603 Complement factor H P05156 Complement factor I P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain P02751 Fibronectin P04196 Histidine-rich glycoprotein P19827 Inter-alpha-trypsin inhibitor heavy chain H1 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 P01042 Kininogen-1 P36955 Pigment epithelium-derived factor P05155 Plasma protease C1 inhibitor P00747 Plasminogen P02768 Serum albumin P02743 Serum amyloid P-component P07225 Vitamin K-dependent protein S P04004 Vitronectin P01876 Ig alpha-1 chain C region P01857 Ig gamma-1 chain C region P01859 Ig gamma-2 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions P01871 Ig mu chain C region

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 7of9 100-nm amine-modified

P01591 Immunoglobulin J chain Ig kappa chain V-II region

50-nm carboxyl-modified P02765 Alpha-2-HS-glycoprotein P01019 Angiotensinogen P01008 Antithrombin-III P02647 Apolipoprotein A-I P02652 Apolipoprotein A-II P06727 Apolipoprotein A-IV P02654 Apolipoprotein C-I P02656 Apolipoprotein C-III P02649 Apolipoprotein E P02749 Beta-2-glycoprotein 1 P61769 Beta-2-microglobulin P04003 C4b-binding protein alpha chain P10909 Clusterin P03951 Coagulation factor XI P01024 Complement C3 P0C0L4 Complement C4-A P0C0L5 Complement C4-B P13671 Complement component C6 P08603 Complement factor H Q03591 Complement factor H-related protein 1 P02671 Fibrinogen alpha chain P02675 Fibrinogen beta chain P02679 Fibrinogen gamma chain P02751 Fibronectin O75636 Ficolin-3 P00738 Haptoglobin P04196 Histidine-rich glycoprotein P19827 Inter-alpha-trypsin inhibitor heavy chain H1 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 Q14624 Inter-alpha-trypsin inhibitor heavy chain H4 P01042 Kininogen-1 P18428 Lipopolysaccharide-binding protein P61626 Lysozyme C P19105 Myosin regulatory light chain 2 P02775 Platelet basic protein P02776 Platelet factor 4 P07737 Profilin-1 Q92954 Proteoglycan-4 P02768 Serum albumin P02766 Transthyretin P02774 Vitamin D-binding protein P04004 Vitronectin P01857 Ig gamma-1 chain C region P01834 Ig kappa chain C region P01842 Ig lambda chain C regions Ig kappa chain V-II region

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 8of9 Table S3. Zeta potentials of AAC (؊)- and DMPA (؉)-modified copolymer particles in PBS Zeta potential, mV*

10,000 AAC Ϫ4.8 Ϯ 1.6 100,000 AAC Ϫ2.0 Ϯ 1.8 10,000 DMPA Ϫ2.8 Ϯ 2.4 100,000 DMPA Ϫ2.0 Ϯ 0.9

*Measured in PBS at 8 Ϯ 2°C.

Other Supporting Information Files

Table S1 (XLS)

Lundqvist et al. www.pnas.org/cgi/content/short/0805135105 9of9