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Question Bank KONGUNADU ARTS AND SCIENCE COLLEGE (AUTONOMOUS) COIMBATORE-641029 QUESTION BANK SUBJECT CODE: (15UBT612) TITLE OF THE PAPER: C.P.12 GENOMICS, PROTEOMICS AND BIOINFORMATICS DEPARTMENT OF BIOTECHNOLOGY APRIL 2019 1 Prepared by L SURENDRAN GUEST LECTURER Department of Biotechnology, Kongunadu Arts & Science College, Coimbatore - 29. 2 Kongunadu Arts & Science College (Autonomous) Department of Biotechnology, Question Bank C.P.12GENOMICS, PROTEOMICS AND BIOINFORMATICS (15UBT612) CONTENTS S.No Content Page No. 1 SECTION A 4 2 SECTION B 12 3 SECTION C 13 3 SECTION A 1. STS markers are assayed by ---------against a library of large insert clones. (a) PCR (b) SNP (c) fingerprinting (d) SAGE. 2. On the genetic map, distances between markers are measured in terms of. (a)Hertz (b) centimorgans (c) decibel (d) both a &b 3. Genetic mapping resolution has been increased through the application of ---------. (a) RNA technology (b) Microarray (c) recombinant DNA technology (d) PAGE 4. -------was the first marker to be used (a) Genes (b) RFLP (c) SSLP (d) snp 5.-----------were the first type of DNA marker to be studied. (a) RFLP (b) SSLP (c) SNP (d) STS 6. Different types of physical maps vary in their -----------. (a)degree of resolution (b)Density (c)distance (d)Length 7. The FISH is Mapping technique to study the (a) Gene location on a genome (b) Protein location (c) Amino acid location (d) Genome counting 8. The Chain termination method is related to (a) DNA sequencing (b) Protein Quantification (c) DNA Quantification (d) Colorimetric assay 9. The genome of an organism is its whole --------------hereditary information. (a) Hereditary (b) Structure (c) Complementary (d) Activity 10. The term genome was adapted in --------- (a) Johanson 1909 (b)Valter 1920 (c) Hans Winkler1920 (d)Hooke 1922 11. -------------is the first organism to be sequenced (a) S.Cerevisiae (b) E.coli (c) H.Influenzae (d)Mycoplasma Genitalium 12. SNP is a type of (a) Mutation (b) Restriction (c) Mapping (d) Ligation 13. Simple Sequence Length Polymorphism technique related to (a) VNTR (b) Mini satellite (c) Microsatellite (d) Both b and c 4 14. Some restriction enzymes cut the DNA very infrequently are called----- (a).rare- cutters (b).absolute cutters (c) SNP (d).RFLP 15. ---------Uses restriction map data to determine which fragments have a specific sequence in common and therefore overlap. (a).FISH (b)SNP (c) fingerprinting (d)SAGE 16. ---------- Techniques can be used to fill in the gaps. (a).FISH (b)SNP (c) fingerprinting (d) DNA probe. 17. The lowest- resolution physical map is the ------------map. (a) Integrated (b) Transcript (c) Chromosomal (d) Linkage 18. FISH was developed by---------- (a)Watson (b) John teller (c) Heiskanen (d) Peter 19. Individual unassembled sequence reads, produced by sequencing of clones containing DNA inserts are called------------ sequence. (a)Raw (b) Nucleotide (c) Transcribed (d) Translate 20. ---------- have long pedigrees but which were rarely applied in the pre-genomics era (a) Protein Electrophoresis (b)Gel Electrophoresis (c)Blotting (d)Sequencing UNIT II 21. A------------is an array of small glass slide containing Probes. (a)Alignment (b) Microarray (c) SNP (d) MSA 22. DNA Microarrays works on the principal of --------------. (a) Base Pairing (b) Mutation (c) Replication (d) Alignment 23. The composition of a transcriptome can be assayed by -------------. (a)Gel Electrophoresis (b) SAGE (c) SNP (d) RFLP 24.The relative abundance of each strain in the pool is measured on an --------array. (a) Oligonucleotide (b) Motif (c) RNA (d) Microarray 25. ----------------is the process by which inheritable information from a gene is made into a functional gene product. (a) Polymorphism (b) Gene Expression (c) Replication (d) Gene Regulation 5 26. Examination of the transcriptome Writes an accurate indication of which genes are active in a particular cell. (a)Active (b) Inactive (c) Discrete (d) Continuous 27. The expression of many genes is regulated after -----------------. (a)Transcription (b) Translation (c) Replication (d) Duplication 28. Proteins are activated by (a)Phosphorylation (b) Acetylation (c) Sumoylation (d) Glycosylation 29. Long chain of amino acids are called (a)Protein (b) Amino acids (c) Polypeptide (d) Poly Amino acids 30. Mass spectrometry is used to study (a)DNA sequence (b) Compound molecular weight (c)Protein sequence (d) None of these 31. ----------------is the process by which inheritable information from a gene is made into a functional gene product. (a) Polymorphism (b) Gene expression (c) Replication (d) Gene Regulation 32. At first the transcription process is related to (a) Gene transfer (b) c-DNA (c) Pre m-RNA Synthesis (d) Mature m-RNA 33. In DNA Microarray uses fluorescent dyes namely (a) Propidium iodide (b) Cyn 3 + Cyn 5 (c) Sybr green (d) Ethidium bromide 34. In the Microarray, a fluorescent emission of a gene analyzed by (a) Spot analysis (b) Spot and image analysis (c) Dot blots (d) Northern blot 35. DNA is associated with DNA-binding proteins called -------. (a)Histones (b) Nucleosome (c) Chromatid (d) Plastids 36. -------are transcripts of protein-coding genes and hence are translated into protein. (a)mRNAs (b) tRNAs (c) rRNA (d) HnRNAs 37. The ---------structure refers to the different conformations that can be taken up by the polypeptide. (a)Primary (b) Secondary (c) Tertiary (d) Quaternary 38. X ray diffraction analysis is to find the (a)Protein Structure (b) Structure comparison (c) DNA Structure (d) Both A and C 6 39. NMR analysis is to find the (a)Protein Structure (b) Structure comparison (c) DNA Structure (d) Both A and C 40. MALDI and SELDI are the techniques to (a) To sequencing of DNA (b) to characterize the protein (c) To quantification of protein (d) to sequencing of amino acids UNIT III 41. Who among the following invented the small pox vaccine? (a)Edward Jenner (b) Leuwis Pasteur (c) Karymullins (d) Sanger 42. The drug which is not used in the treatment of tuberculosis a) Rifampin b) Isoniazid c) DARQ d) Amantadine 43. A good example of drug protein interaction (a) Nutlin-mdm2 binding (b) Nutlin-p21 (c) Doxorubin mdm4 (d) Mdm4 ampicillin 44. Two drugs responsible for inhibition of mdm-2 binding (a)Nutlin-AMG232 (b) Nutlin- doxorubicin (c) Doxorubin alone (d) Nutlin- amoxlyin 45. Monoclonal antibodies made by (a)Single modification (b) multiple modification (c) Combination (d) Protein interaction 46. Drug designing was done in previous centuries by (a)Animal studies (b) Human studies (c) In-Vitro studies (d) In-silico studies Drug discovery is relation to (a)Inventing new therapeutic molecular (b) Antibody (c) Vaccine (d) Invention 47. The ------- is the multiple alignments of several protein sequences. (a) Motif (b) Profile (c) Domain (d) Clusters 48. ---------- have long pedigrees but which were rarely applied in the pre-genomics er(a) (a) Protein electrophoresis (b) Gel electrophoresis (c) Blotting (d) Sequencing The yeast is commonly called as the ------------. (a)Baker’s yeast (b) Spore yeast (c) Buds (d) Dead cells. 49. Yeast has been introduced as an experimental organism in -----------. (a)1982 (b)1981 (c)1880 (d)2000 50. Yeast is ----------- fungi. (a) Multicellular (b) Unicellular (c) Parasite (d) Cellular 7 51. The bait and prey are the two protein molecules employed in the (a) Yeast two hydrid system (b) ELISA method (c) Structure validation (d) Sequencing 52. First dimension electrophoresis of protein is called (a) Differential electrophoresis (b) Gel electrophoresis (c) Iso-electric focusing (d) none of them 53. Immunomics is study of (a)Antibody (b) Antibody interaction (c) Antibody Antigen Titer (d) Drug Discovery 54. Vaccinomics is study of (a)Antigen types (b) Epitope (c) Subunit (d) Antigen antibody interaction 55. Pharmacogenomics is related to (a)Pharma proteins (b) Immune proteins (c) Therapeutic proteins (d) Antibody 56. Drug discovery is related with the designing of (a)Drugs (b) Therapeutic drugs (c) Monoclonal antibody (d) Chemical drugs 57. In Genomics, the term “Drug - target” is related to (a) To find drug binding site (b) To find drug function (c) To predict drug activation (d) sequencing of amino acids 58. As early as in 1980, ----- was used to produce Hepatitis B vaccine. (a)Humans (b) S. Cerevisiae (c) Mus (d) Fruit fly 59. In DNA Microarray gene were hybridized with (a) tRNA (b) mRNA (c) cDNA (d) Genomic DNA 60. SAGE will be used for prediction of (a) Gene expression (b) Protein expression (c) Protein interaction (d) Chemical structure UNIT IV 61. Developmental biology of gene expression is related to (a) Gene expression at various stages of embryo development (b) Types of production of cells (c) Human embryo development (d) Protein production 62. Regulatory genes are (a)Regulating cells (b) Killing cells (c) Controlling cell cycle (d) Controlling cell signal 8 63. Stanford microarray data base is storing data of (a)Gene expression (b) Lipid production (c) Protein expression (d) Antibody production 64. Microarrays are used for the studying of (a)10 genes b, 100 genes (c)500 genes (d)1000genes 65. In Microarray, tagging is term using for (a) Group of genes (b) group of exons (c) group of intron (d) group of pseudo genes 66. OMIM database was initiated in the early 1960s by ----------. (a)Dr. Victor A (b) Dr. James (c) Dr. Wilson (d) Dr. Walter. 67. In Genomics and proteomics, the data normalization will be done (a) To avoid the error and noise (b) To remove normal data (c) To remove sample data (d) to adjustment of data 68. --------is the NIH genetic sequence database, a collection of all publicly available DNA sequences. (a)NCBI (b) GenBank (c) EXPASY (d) Both of a & b 69. The-------------- database is intended to provide information on the sequences. (a)NCBI (b) GenBank (c) EXPASY (d) ncRNA 70. ------- is an integrated knowledge database dedicated to non-coding RNAs (a) NONCODE (b) Profile (c) Domain (d) Clusters 71.
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