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Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 2000 Pathogenesis of Bartonella Henselae in the Domestic Cat: Use of a PCR-based Assay for the Detection and Differentiation of B. Henselae Genotype I and Genotype II in Chronically Infected Cats. Alma Faye Roy Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Roy, Alma Faye, "Pathogenesis of Bartonella Henselae in the Domestic Cat: Use of a PCR-based Assay for the Detection and Differentiation of B. Henselae Genotype I and Genotype II in Chronically Infected Cats." (2000). LSU Historical Dissertations and Theses. 7294. https://digitalcommons.lsu.edu/gradschool_disstheses/7294 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6* x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. Bell & Howell Information and Learning 300 North Zeeb Road, Ann Arbor, Ml 48106-1346 USA 800-521-0600 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PATHOGENESIS OF BARTONELLA HENSELAE IN THE DOMESTIC CAT: USE OF A PCR-BASED ASSAY FOR THE DETECTION AND DIFFERENTIATION OF B. HENSELAE GENOTYPE I AND GENOTYPE H IN CHRONICALLY INFECTED CATS A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Interdepartmental Program in Veterinary Medical Sciences through the Department of Veterinary Microbiology and Parasitology by Alma F. Roy B. S., Louisiana State University, 1968 M. S., College o f St. Francis, 1990 August 2000 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. UMI Number 9984362 __ ___ __ <gi UMI UMI Microform9984362 Copyright 2000 by Bell& Howell Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17. United States Code. Bell & Howell Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, Ml 48106-1346 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. To all of my grandchildren that they may understand the value of education ii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ACKNOWLEDGMENTS I am greatly indebted to my major professor, Dr. R. E. Corstvet, for his constant support and guidance through the pursuit of my Ph.D. He believed in me from the beginning and he inspired me to begin this endeavor. Throughout this process he had eternal optimism and supported me through each step and mountain I had to climb. Because of him, a scientist has emerged where only a laboratory technologist was before. I look to him as to what a good teacher, researcher, and scientist should be. He has helped many people pursue their dreams, I being one of them. I would also like to thank Dr. Johannes Storz for the excellent programs in the Department of Veterinary Microbiology and Parasitology. I have worked in this Department for many years and have seen the support and guidance he has given to many and especially to graduate students. He supported my endeavors as a graduate student despite the fact that I was not the traditional graduate student. I would like to thank Dr. Hollis Utah Cox for all the years we have worked together. I know there were many times in which I failed to meet his expectations. Despite the fact that he wins most of the arguments or discussions we have, I still look forward everyday to seeing him. His knowledge and intelligence probably inspires me to think, question and seek knowledge more than anyone else does. He has given me the space to pursue this degree and I truly appreciate that. I am grateful to the other members of my committee Dr. R. Bauer, Dr. K. O’Reilly, Dr. M. Neer, Dr. M. Groves and Dr. M. Hellberg for all of their support and guidance. I am grateful to Dr. R. Bauer for all of the valuable time he spent in the necropsy room. Dr. Bauer is an excellent pathologist and a great person to challenge, iii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. because there are few things he does not know. Dr. K. O’Reilly deserves special thanks for allowing me to use cats from her research. Many members of her laboratory supported my project by drawing blood and performing western blots. Dr. O’Reilly’s advice and support was always very helpful. Dr. M. Neer, whom I admire as an outstanding clinician, has believed and supported me throughout this endeavor. Dr. Neer has contributed to my program through a clinical approach and with the eyes of a clinician, to him I am appreciative. I am grateful to Dr. Groves, because he is the person who introduced me to Bartonella and sparked my interest in this microorganism. Dr. Groves was one of the first to perceive the impact ofBartonella species in veterinary medicine. Dr. Hellberg has supported my program throughout and I thank him for his cooperation. I would like to thank Ron Tapp, who designed the primer for the PCR assay. Ron and I had many conversations about DNA and PCR assays. I thank Ron for all the support and as a friend and fellow sufferer through the hardships of graduate school. I would also like to thank Dr. Mark Mitchell a fellow graduate student, for all the conversations and his encouragement and all the pats on the back he gave me throughout this process. I would also like to thank Dr. Diana Williams, molecular biologist, and longtime friend, for all the help she gave me through this endeavor. I sought out her expertise of molecular biology on many occasions during this research, and she was always willing to teach and guide me. Diana is a brilliant scientist whose work with mycobacteria is renown and whom I have admired for a longtime. I would like to thank a special group, Jason Farrall, J.P. Ginady, and Lance Mayfield. These guys helped me with the cats and some of the laboratory work. Most iv Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. of all I thank them for all the discussions and their own perspective they brought to this project. I would also like to thank the people in the gene lab, Li Huang, Susan Newman, Valdamir Chouljenko, and Dr. Kousoulous for their guidance in exploring the molecular world. Other individuals who have been instrumental in allowing me to achieve this goal include members of the Louisiana Veterinary Medical Diagnostic Laboratory. They all gave me support and encouragement. Most of all, I am grateful to my husband, John D., without whose love and belief in me I never could have completed this degree. He too has continued with the quest for knowledge in his profession. He has always understood and allowed me to pursue whatever 1 wanted. He is a great husband and friend. He was patient and understanding through all the times and hours spent pursuing this dream. He attended a lot of movies by himself but also got to go on many quail hunts to Texas and Kansas because his wife was occupied. I would like to thank my family for being supportive of my degree. I would like to thank my daughters, Amy and Jennifer, for being such successful adults and beautiful people. Their success allowed me to pursue this degree. I would like to thank Creighton for being such a good husband to my daughter and father to my grandchildren. I would like to thank Madeline and Olivia and one day hope they will understand why their Grannie was always in school. I hope that one day they will pursue their dreams maybe even being a scientist. Thanks also go to my parents, Alfred and Hilda Mix for being supportive and, hopefully, proud of their daughter. Over the V Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. years, they have been great parents and role models for their children. There were many hours taken away from family and I thank them for understanding. vi Reproduced with permission of the copyright owner.

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