DOCSLIB.ORG

Wo 2011/009849 A2

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Wo 2011/009849 A2 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date 27 January 2011 (27.01.2011) WO 2011/009849 A2 (51) International Patent Classification: (74) Common Representative: BASF SE; 67056 Lud C12P 7/62 (2006.01) wigshafen (DE). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/EP2010/060458 kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (22) Date: International Filing CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, 20 July 2010 (20.07.2010) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (26) Publication Language: English ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (30) Priority Data: NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 09166015.9 2 1 July 2009 (21 .07.2009) EP SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, 61/227,797 23 July 2009 (23.07.2009) US TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicants (for all designated States except US): BASF (84) Designated States (unless otherwise indicated, for every SE [DE/DE]; 67056 Ludwigshafen (DE). MAX- kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgarten- ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, str. 8, 80539 Mϋnchen (DE). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (72) Inventors; and LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, (75) Inventors/Applicants (for US only): STURMER, Rain- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, er [DE/DE]; Hauptstrasse 153, 67127 Rόdersheim- GW, ML, MR, NE, SN, TD, TG). Gronau (DE). SCHNEIDER, Nina [DE/DE]; Carl-Blos- Str. 3, 77654 Offenburg (DE). BOY, Matthias [DE/DE]; Published: Ernst-Ludwig-Str. 39 a, 64625 Bensheim (DE). — without international search report and to be republished ACHATZ, Brigitte [DE/DE]; Windeckstr. 26, 681 63 upon receipt of that report (Rule 48.2(g)) Mannheim (DE). RABUS, RaIf [DE/DE]; Hinrich- — with sequence listing part of description (Rule 5.2(a)) Schmidt-Str. 52, 26 160 Bad Zwischenahn (DE). HEI- DER, Johann [DE/DE]; Spiegelslustweg 24b, 35039 Marburg (DE). (54) Title: METHOD FOR PREPARING OPTICALLY ACTIVE HYDROXY ACID ESTERS (57) Abstract: The invention relates to a method for preparing optically active 2-hydroxy acid ester derivatives of the formula Rl- C(OH)-C(O)-O-R2 (I),comprising the bringing into contact of 2-oxo acid ester derivatives of the formula R1-C(O)-C(O)-O-R2 (II) with an enzyme (E) selected from the class of dehydrogenases, in the presence of reduction equivalents, where the compound of the formula (II) is enzymatically reduced to the compound of the formula (I), and the reduction equivalents consumed in the course of the reaction are regenerated again by converting a reducing agent (RA) to the corresponding oxidation product (OP) with the help of the enzyme (E). The invention further comprises a dehydrogenase which reduces 2-oxo acid esters in the presence of reduction equivalents to the corresponding S- 2-hydroxy acid esters, and also a nucleic acid encoding the dehydrogenase. Method for preparing optically active hydroxy acid esters The present invention relates to a method for obtaining optically active hydroxy compounds through enantioselective reduction of organic keto compounds and methods for obtaining these hydroxy compounds in the two-phase system using enzymes with dehydrogenase activity. Background of the invention Optically active hydroxy compounds, such as, for example, ethyl 2-hydroxy-4- phenylbutyrate, of very high enantiomer purity are important precursors in the synthesis of drugs, in particular inhibitors of the so-called angiotensin converting enzyme (ACE), which are used for treating patients with hypertension. Many of these inhibitors, such as enalapril, ramipril, cilazapril, quinapril and lysinapril, have a common general structural feature which is responsible for improved application properties. The feature common to such inhibitors is the S-enantiomer form of the 2-amino-4-phenylbutyrate with the structural formula (Ia). The S-2-hydroxy-4-phenylbutyric acid of the formula Ib (R enantiomer) is used for preparing isomeric compounds. Obtaining chiral compounds through stereospecific microbiological reduction is known (for an overview cf. Simon et al., Angew. Chemie 97, 541 , 1985). The biocatalysts used are often intact microorganisms, for example fungi (e.g. Mucor, Geotrichum, Saccharomyces, Candida) or bacteria (e.g. Proteus, Pseudomonas). It is also possible to use microbial extracts. Electron donors are, for example, carbohydrates (e.g. glucose), formate, ethanol, hydrogen or the cathode of an electrochemical cell. Reduction of the substrate takes place through a reductase, e.g. through a substrate-specific dehydrogenase. In general, the reduction equivalents required by the reductase are supplied by a coenzyme, e.g. by pyridine nucleotides such as NADH (nicotinamide adenine dinucleotide) and NADPH (nicotinamide adenine dinucleotide phosphate) or by flavin nucleotides such as FMNH (flavin mononucleotide) and FADH (flavin adenine dinucleotide). The reduced nucleotides for their part are usually formed in a series of enzyme-catalyzed steps with the formation of competing electron acceptors or by electron transfer via natural or synthetic mediators (e.g. ferredoxin, viologens). Also known are final reductases which can absorb electrons directly from the mediators. For example, Lacerda et al., Tetrahedron: Asymmetry 17, 2006, pages 1186-1 188, describes a method for the microbial reduction of 2-oxo-4-phenylbutyrates using various bacterial strains. The European patent specification EP 0 347 374 describes a method of preparing R-2- hydroxy-4-phenylbutyric acid in which the substrate is reduced with the enzyme D-lactate dehydrogenase from Staphylococcus epidermidis in the presence of an electron donor and an enzyme-substrate system for regenerating the electron donor. Furthermore, WO2005/049816 discloses a NADPH-dependent dehydrogenase from Metschnikowia zobellii which, in the presence of water and NADPH, catalyzes the stereoselective reduction of carbonyl compounds to the corresponding chiral hydroxy compounds. EP 0 645 453 discloses an enantioselective alcohol dehydrogenase which is suitable for the reduction of organic keto compounds to the corresponding hydroxy compounds, this reduction leading enantioselectively to the corresponding R compounds. Short description of the Invention: The object of the invention was to find a route for the enantioselective reduction of 2-oxo acid esters, in particular ethyl 2-oxo-4-phenylbutyrate, wherein the reaction method should lead as quantitatively as possible to the product by a cost-effective route. This object was achieved by the surprising finding that enzymes with dehydrogenase activity, in particular those which can be prepared from microorganisms of the genus Azoarcus, are capable of the enantioselective catalysis of the above reaction with simultaneous cofactor regeneration. The invention firstly provides a method for preparing optically active 2-hydroxy acid ester derivatives of the formula (I), R1-C(OH)-C(O)-O-R2 or a salt thereof, wherein R 1 and R2, independently of one another, are a) -(C1-C20)-alkyl, in which alkyl is straight-chain or branched, b) -(C2-C20)-alkenyl, in which alkenyl is straight-chain or branched and comprises one, two, three or four double bonds depending on chain length, c) -(C2-C20)-alkynyl, in which alkynyl is straight-chain or branched and optionally comprises one, two, three or four triple bonds, d) -(C6-C14)-aryl, e) -(C1-C8)-alkyl-(C6-C14)-aryl, f) -(C5-C14)-heterocycle which is unsubstituted or mono- to trisubstituted by halogen, hydroxyl, amino or nitro, or g) -(C3-C7)-cycloalkyl, wherein the residues specified above under a . to g . are unsubstituted or are mono- to trisubstituted, independently of one another, by 1. -OH, 2 . halogen, such as fluorine, chlorine, bromine or iodine, 3 . -NO2, 4 . -C(O)-O-(CI -C20)-alkyl, in which alkyl is straight or branched and is unsubstituted or mono- to trisubstituted by halogen, hydroxyl, amino or nitro, or 5 . -(C5-C14)-heterocycle which is unsubstituted or mono- to trisubstituted by halogen, hydroxyl, amino or nitro the method comprising the bringing into contact of 2-oxo acid ester derivatives of the formula (II) R1-C(O)-C(O)-O-R2 with an enzyme (E) selected from the class of the dehydrogenases, in the presence of reduction equivalents, wherein the compound of the formula (II) is enzymatically reduced to the compound of the formula (I), and the reduction equivalents consumed in the course of the reaction are regenerated again by converting a reducing agent (RA) to the corresponding oxidation product (OP) with the help of the enzyme (E). Depending on the dehydrogenase used, the -OH group of the formula (I) is in the S configuration (Ia) or in the R configuration (Ib) relative to the carbon atom to which it is bonded. Enzymes (E) suitable according to the invention are in particular the enzymes of the families of the aldo-keto reductases of the aldo-keto reductase superfamily (K.M.Bohren, B.Bullock, B.Wermuth and K.H.Gabbay J.Biol. Chem. 1989, 264, 9547-9551) and of the short-chain alcohol dehydrogenases/reductases (SDR). The latter enzyme group is described in detail, for example, in H.Jδrnvall, B.Persson, M.Krook, S.Atrian, R.Gonzalez-Duarte, J.Jeffery and D.Ghosh, Biochemistry, 1995, 34, pp.
Load more

Recommended publications
  • Isotope Array Analysis of Rhodocyclales Uncovers Functional Redundancy and Versatility in an Activated Sludge
    The ISME Journal (2009) 3, 1349–1364 & 2009 International Society for Microbial Ecology All rights reserved 1751-7362/09 $32.00 www.nature.com/ismej ORIGINAL ARTICLE Isotope array analysis of Rhodocyclales uncovers functional redundancy and versatility in an activated sludge Martin Hesselsoe1, Stephanie Fu¨ reder2, Michael Schloter3, Levente Bodrossy4, Niels Iversen1, Peter Roslev1, Per Halkjær Nielsen1, Michael Wagner2 and Alexander Loy2 1Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark; 2Department of Microbial Ecology, University of Vienna, Wien, Austria; 3Department of Terrestrial Ecogenetics, Helmholtz Zentrum Mu¨nchen—National Research Center for Environmental Health, Neuherberg, Germany and 4Department of Bioresources/Microbiology, ARC Seibersdorf Research GmbH, Seibersdorf, Austria Extensive physiological analyses of different microbial community members in many samples are difficult because of the restricted number of target populations that can be investigated in reasonable time by standard substrate-mediated isotope-labeling techniques. The diversity and ecophysiology of Rhodocyclales in activated sludge from a full-scale wastewater treatment plant were analyzed following a holistic strategy based on the isotope array approach, which allows for a parallel functional probing of different phylogenetic groups. Initial diagnostic microarray, comparative 16S rRNA gene sequence, and quantitative fluorescence in situ hybridization surveys indicated the presence of a diverse community, consisting of an estimated number of 27 operational taxonomic units that grouped in at least seven main Rhodocyclales lineages. Substrate utilization profiles of probe-defined populations were determined by radioactive isotope array analysis and microautoradiography-fluorescence in situ hybridization of activated sludge samples that were briefly exposed to different substrates under oxic and anoxic, nitrate-reducing conditions.
    [Show full text]
  • Anaerobic Degradation of Steroid Hormones by Novel Denitrifying Bacteria
    Anaerobic degradation of steroid hormones by novel denitrifying bacteria Von der Fakultät für Mathematik, Informatik und Naturwissenschaften der Rheinisch- Westfälischen Technischen Hochschule Aachen zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigte Dissertation vorgelegt von Diplom-Biologe Michael Fahrbach aus Bad Mergentheim (Baden-Württemberg) Berichter: Professor Dr. Juliane Hollender Professor Dr. Andreas Schäffer Tag der mündlichen Prüfung: 12. Dezember 2006 Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online verfügbar. Table of Contents 1 Introduction.....................................................................................................................1 1.1 General information on steroids ...............................................................................1 1.2 Steroid hormones in the environment.......................................................................2 1.2.1 Natural and anthropogenic sources and deposits ............................................2 1.2.2 Potential impact on the environment ................................................................3 1.2.3 Fate of steroid hormones..................................................................................4 1.3 Microbial degradation of steroid hormones and sterols............................................5 1.3.1 Aerobic degradation..........................................................................................5 1.3.2 Anaerobic degradation......................................................................................7
    [Show full text]
  • Biodegradation of Aromatic Hydrocarbons in Oxygen-Limited Groundwater
    TECHNISCHE UNIVERSITÄT MÜNCHEN Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt Lehrstuhl für Mikrobologie Biodegradation of aromatic hydrocarbons in oxygen-limited groundwater Lauren Bradford Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzende(r): Hon.-Prof. Dr. Michael Schloter Prüfer der Dissertation: 1. Prof. Dr. Tillmann Lüders 2. Prof. Dr. Wolfgang Liebl Die Dissertation wurde am 27.01.2020 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 08.06.2020 angenommen. 1 Abstract Hydrocarbon contamination of groundwater is a global problem, negatively impacting human health and ecosystem functioning. One of the main ways to mitigate this issue is bioremediation, the use of microorganisms to sequester or break down pollutants. Bacteria capable of metabolizing hydrocarbons are widespread in the environment, including groundwater. They rely on a variety of metabolic and respiratory strategies, distinguished primarily by the availability of oxygen. Oxygen can act as a terminal electron acceptor (TEA) during respiration, but also as a metabolic co-substrate during the breakdown of stable compounds like aromatic hydrocarbons. Anaerobic degradation relies on alternative TEAs and mechanisms of aromatic destabilization. Strictly aerobic and anaerobic hydrocarbon degradation have been well studied in recent decades, but hot spots of degradation occur at the fringes of contaminant plumes, where mixing of oxygen and other TEAs create dynamic, TEA- limited conditions. Microaerobes and other microbes with specialized strategies that could offer advantages in such conditions have been poorly investigated, despite their probable contribution to pollutant degradation in environmentally-relevant situations.
    [Show full text]
  • The Succession Pattern of Bacterial Diversity in Compost Using Pig
    bioRxiv preprint doi: https://doi.org/10.1101/674069; this version posted June 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 The succession pattern of bacterial diversity in compost using pig 2 manure mixed with wood chips analyzed by 16S rRNA gene analysis 3 Zhengfeng Li4¶, Yan Yang1,2,3¶, Yuzhen Xia5, Tao Wu4, Jie Zhu4, Zhaobao Wang1,2,3*, Jianming 4 Yang1,2,3* 5 1 Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, 6 Qingdao Agricultural University, Qingdao, China 7 2 Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 8 Qingdao, China 9 3 College of Life Sciences, Qingdao Agricultural University, Qingdao, China 10 4 China Tobacco Yunnan Industrial Co., Ltd., Kunming, China 11 5 Hongta Tobacco (Group) Co., Ltd., Yuxi, China 12 13 14 * Corresponding authors 15 Email: [email protected] (ZW), 16 [email protected] (JY) 17 18 ¶ These authors are co-first authors on this work. 19 20 21 22 23 24 bioRxiv preprint doi: https://doi.org/10.1101/674069; this version posted June 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 25 Abstract 26 The pig manure mixed with wood chips and formed compost by means of 27 fermentation.
    [Show full text]
  • The Succession Pattern of Bacterial Diversity in Compost Using Pig
    bioRxiv preprint doi: https://doi.org/10.1101/674069; this version posted June 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 The succession pattern of bacterial diversity in compost using pig 2 manure mixed with wood chips analyzed by 16S rRNA gene analysis 3 Zhengfeng Li4¶, Yan Yang1,2,3¶, Yuzhen Xia5, Tao Wu4, Jie Zhu4, Zhaobao Wang1,2,3*, Jianming 4 Yang1,2,3* 5 1 Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, 6 Qingdao Agricultural University, Qingdao, China 7 2 Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 8 Qingdao, China 9 3 College of Life Sciences, Qingdao Agricultural University, Qingdao, China 10 4 China Tobacco Yunnan Industrial Co., Ltd., Kunming, China 11 5 Hongta Tobacco (Group) Co., Ltd., Yuxi, China 12 13 14 * Corresponding authors 15 Email: [email protected] (ZW), 16 [email protected] (JY) 17 18 ¶ These authors are co-first authors on this work. 19 20 21 22 23 24 bioRxiv preprint doi: https://doi.org/10.1101/674069; this version posted June 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 25 Abstract 26 The pig manure mixed with wood chips and formed compost by means of 27 fermentation.
    [Show full text]
  • Outline Release 7 7C
    Taxonomic Outline of Bacteria and Archaea, Release 7.7 Taxonomic Outline of the Bacteria and Archaea, Release 7.7 March 6, 2007. Part 4 – The Bacteria: Phylum “Proteobacteria”, Class Betaproteobacteria George M. Garrity, Timothy G. Lilburn, James R. Cole, Scott H. Harrison, Jean Euzéby, and Brian J. Tindall Class Betaproteobacteria VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.16162 Order Burkholderiales VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.1617 Family Burkholderiaceae VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.1618 Genus Burkholderia VP Yabuuchi et al. 1993. GOLD ID: Gi01836. GCAT ID: 001596_GCAT. Sequenced strain: SRMrh-20 is from a non-type strain. Genome sequencing is incomplete. Number of genomes of this species sequenced 2 (GOLD) 1 (NCBI). N4Lid DOI: 10.1601/nm.1619 Burkholderia cepacia VP (Palleroni and Holmes 1981) Yabuuchi et al. 1993. <== Pseudomonas cepacia (basonym). Synonym links through N4Lid: 10.1601/ex.2584. Source of type material recommended for DOE sponsored genome sequencing by the JGI: ATCC 25416. High-quality 16S rRNA sequence S000438917 (RDP), U96927 (Genbank). GOLD ID: Gc00309. GCAT ID: 000301_GCAT. Entrez genome id: 10695. Sequenced strain: ATCC 17760, LMG 6991, NCIMB9086 is from a non-type strain. Genome sequencing is completed. Number of genomes of this species sequenced 1 (GOLD) 1 (NCBI). N4Lid DOI: 10.1601/nm.1620 Pseudomonas cepacia VP (ex Burkholder 1950) Palleroni and Holmes 1981. ==> Burkholderia cepacia (new combination). Synonym links through N4Lid: 10.1601/ex.2584. Source of type material recommended for DOE sponsored genome sequencing by the JGI: ATCC 25416. High- quality 16S rRNA sequence S000438917 (RDP), U96927 (Genbank).
    [Show full text]
  • Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Copenhagen University Research Information System Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota Bradford, Lauren M.; Vestergaard, Gisle; Táncsics, András; Zhu, Baoli; Schloter, Michael; Lueders, Tillmann Published in: Frontiers in Microbiology DOI: 10.3389/fmicb.2018.02696 Publication date: 2018 Document version Publisher's PDF, also known as Version of record Document license: CC BY Citation for published version (APA): Bradford, L. M., Vestergaard, G., Táncsics, A., Zhu, B., Schloter, M., & Lueders, T. (2018). Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota. Frontiers in Microbiology, 9, [2696]. https://doi.org/10.3389/fmicb.2018.02696 Download date: 09. apr.. 2020 fmicb-09-02696 November 13, 2018 Time: 12:54 # 1 ORIGINAL RESEARCH published: 13 November 2018 doi: 10.3389/fmicb.2018.02696 Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota Lauren M. Bradford1, Gisle Vestergaard2,3, András Táncsics4, Baoli Zhu1, Michael Schloter4 and Tillmann Lueders1* 1 Institute of Groundwater Ecology, Helmholtz Zentrum München, Neuherberg, Germany, 2 Section of Microbiology, University of Copenhagen, Copenhagen, Denmark, 3 Research Unit Comparative Microbiome Analysis, Helmholtz Zentrum Edited by: München, Neuherberg, Germany, 4 Regional University Center of Excellence in Environmental Industry, Szent István Zofia Piotrowska-Seget, University, Gödöllö, Hungary University of Silesia of Katowice, Poland Reviewed by: While most studies using RNA-stable isotope probing (SIP) to date have focused on Siavash Atashgahi, ribosomal RNA, the detection of 13C-labeled mRNA has rarely been demonstrated.
    [Show full text]
  • The Geomicrobiology of Cementitious Radioactive Waste
    The Geomicrobiology of Cementitious Radioactive Waste A thesis submitted to The University of Manchester for the degree of Doctor of Philosophy in the Faculty of Engineering and Physical Sciences 2014 Adam John Williamson School of Earth, Atmospheric and Environmental Sciences Table of Contents Table of Contents .............................................................................................................. 2 List of Figures ................................................................................................................... 8 List of Tables................................................................................................................... 13 Abbreviations .................................................................................................................. 16 Abstract ........................................................................................................................... 18 Declaration ...................................................................................................................... 19 Copyright Statement ....................................................................................................... 20 Acknowledgments .......................................................................................................... 21 The Author ..................................................................................................................... 22 1. Introduction and Thesis Content ...........................................................................
    [Show full text]
  • Rapid Startup of Simultaneous Nitrogen and Phosphorus Removal (SNPR) Process and the Bacterial Community Dynamics in a GSBR
    Pol. J. Environ. Stud. Vol. 28, No. 4 (2019), 2931-2940 DOI: 10.15244/pjoes/92705 ONLINE PUBLICATION DATE: 2019-03-05 Original Research Rapid Startup of Simultaneous Nitrogen and Phosphorus Removal (SNPR) Process and the Bacterial Community Dynamics in a GSBR Xin Xin*, Ziling Wang College of Resources and Environment, Chengdu University of Information Technology, Chengdu, China Received: 14 March 2018 Accepted: 29 June 2018 Abstract This study inoculated aerobic granular sludge (AGS) in a sequencing batch reactor (SBR) treatment for low carbon nitrogen (COD/N) ratio wastewater, and gradually reduced the DO concentration in order to achieve the rapid startup of the simultaneous nitrogen and phosphorous removal (SNPR) process. Meanwhile, the microbial community dynamics at different DO levels were analyzed by high-throughput sequencing. The removal efficiencies of total nitrogen (TN) and phosphorus (TP) were significantly affected as different dissolved oxygen (DO) concentrations (2.0,1.2 and 0.8 mg/L) in stages I, II and III, respectively. When DO concentration was reduced to 0.8mg/L (stage III), the SNPR process was successfully implemented and the removal efficiencies of TN and TP were up to 77.30% and 85.78%, respectively. A total of 40,983 effective 16S rRNA gene sequences were generated from four samples (1-4) that widely represented microbial community diversity. The dominant phyla transformed from Candidate_division_TM7 (the relative abundance of 68.08%) and proteobacteria (25.78%) to Firmicutes (47.57%) and proteobacteria (41.49%) when DO concentration was decreased from 2.0 mg/L (stage I) to 0.8 mg/L(stage III).
    [Show full text]
  • Aalborg Universitet Single-Cell Ecophysiology of Key Microbial Taxa
    Aalborg Universitet Single-cell ecophysiology of key microbial taxa in wastewater treatment systems A trip to unveil the microbial "dark matter" Petriglieri, Francesca Publication date: 2020 Document Version Publisher's PDF, also known as Version of record Link to publication from Aalborg University Citation for published version (APA): Petriglieri, F. (2020). Single-cell ecophysiology of key microbial taxa in wastewater treatment systems: A trip to unveil the microbial "dark matter". Aalborg Universitetsforlag. Ph.d.-serien for Det Ingeniør- og Naturvidenskabelige Fakultet, Aalborg Universitet General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research. ? You may not further distribute the material or use it for any profit-making activity or commercial gain ? You may freely distribute the URL identifying the publication in the public portal ? Take down policy If you believe that this document breaches copyright please contact us at [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from vbn.aau.dk on: September 23, 2021 TAXA IN WASTEWATER TREATMENT SYSTEMS TREATMENT WASTEWATER IN TAXA MICROBIAL KEY OF ECOPHYSIOLOGY SINGLE-CELL SINGLE-CELL ECOPHYSIOLOGY OF KEY MICROBIAL TAXA IN WASTEWATER TREATMENT SYSTEMS A TRIP TO UNVEIL THE MICROBI AL “DARK MAT TER” BY FRANCESCA PETRIGLIERI DISSERTATION SUBMITTED 2020 FRANCESCA PETRIGLIERI FRANCESCA SINGLE-CELL ECOPHYSIOLOGY OF KEY MICROBIAL TAXA IN WASTEWATER TREATMENT SYSTEMS A TRIP TO UNVEIL THE MICROBIAL “DARK MATTER” By Francesca Petriglieri Dissertation submitted August 2020 .
    [Show full text]