(2)-9-Pentacosene - Contact Sex Pheromone of the Locust Borer, Megacyllene Robinbe Matthew D
Chemoeeology 13: 135-141 (2003) 0937-7w/03/030 135-7 O BirkhIuser Verlag, Basel, 2003 (2)-9-Pentacosene - contact sex pheromone of the locust borer, Megacyllene robinbe Matthew D. Ginzel', Jocelyn G. Milla9 and Lawrence M. Hanks1 'Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 *Depament of Entomology, University of California, Riverside, CA 92521 Summary. Male locust borers, Megacyllene robiniae from desiccation (Gibbs 1998). Components of the wax (Forster), responded to females only after contacting them layer also may have a secondary role as contact pheromones with their antennae, indicating that mate recognition was (Blomquist et al. 1996). There is a growing body of evi- mediated by a contact sex pheromone. W-MS analyses of dence that mate recognition in cerambycid beetles is whole-body extracts of males and females determined that the mediated by such contact sex pheromones (Kim et al. 1993; profiles of compounds in the extracts were qualitatively simi- Fukaya et al. 1996, 1997, 2000; Wang 1998; Ginzel et al. lar, but differed considerably in the ratios of compounds 2003; Ginzel & Hanks 2003). We report here the results between sexes. Biological activities of reconstructed blends of studies on a cuticular sex pheromone of the locust borer, Megacyllene robiniae (Forster) (Coleoptera: of the most abundantstraight-chain (nq,, nq4,nC,, nC2&, w methyl-branched (3me-C,,, 3me-C,), and unsaturated Cerambycidae). This black and yellow longhorned beetle is (Z9:C2,, B:C,, Z9:q7)compounds in extracts from females native to temperate North America and causes substantial 58 were assessed in arena bioassays, assessing four distinct steps damage to poorly managed and older black locust trees, in the mating behavior sequence of males (orientation, arrest- Robinia pseudoacacia L., as well as to naturally regenerat- ment, body alignment, mounting and attempting to couple the ing locust trees and those in young plantations (Solomon genitalia).
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