[N1596] Paraburkholderia Mimosarum A
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Arthrobacter Paludis Sp. Nov., Isolated from a Marsh
TAXONOMIC DESCRIPTION Zhang et al., Int J Syst Evol Microbiol 2018;68:47–51 DOI 10.1099/ijsem.0.002426 Arthrobacter paludis sp. nov., isolated from a marsh Qi Zhang,1 Mihee Oh,1 Jong-Hwa Kim,1 Rungravee Kanjanasuntree,1 Maytiya Konkit,1 Ampaitip Sukhoom,2 Duangporn Kantachote2 and Wonyong Kim1,* Abstract A novel Gram-stain-positive, strictly aerobic, non-endospore-forming bacterium, designated CAU 9143T, was isolated from a hydric soil sample collected from Seogmo Island in the Republic of Korea. Strain CAU 9143T grew optimally at 30 C, at pH 7.0 and in the presence of 1 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain CAU 9143T belonged to the genus Arthrobacter and was closely related to Arthrobacter ginkgonis SYP-A7299T (97.1 % T similarity). Strain CAU 9143 contained menaquinone MK-9 (H2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and two unidentified phospholipids as the major polar lipids. The whole-cell sugars were glucose and galactose. The peptidoglycan type was A4a (L-Lys–D-Glu2) and the major cellular fatty T acid was anteiso-C15 : 0. The DNA G+C content was 64.4 mol% and the level of DNA–DNA relatedness between CAU 9143 and the most closely related strain, A. ginkgonis SYP-A7299T, was 22.3 %. Based on phenotypic, chemotaxonomic and genetic data, strain CAU 9143T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter paludis sp. nov. is proposed. The type strain is CAU 9143T (=KCTC 13958T,=CECT 8917T). -
Here to from Here?
ORAL PRESENTATIONS INDEX 1.1 Revisiting genotypic and phenotypic properties as an aid to circumscribe species of the genus Salinispora .......................... 1 1.2 The use of whole genome sequencing to confirm the recognition of M. noduli and M. saelicesensis ........................... 2 1.3 Genome-scale data call for a taxonomic rearrangement of Geodermatophilaceae ................................................................... 3 1.4 Diversity and distribution of sphingomonads ................... 4 1.5 Genome-informed Bradyrhizobium taxonomy: where to from here? .................................................................................... 5 1.6 Divergence and gene flow in Xanthomonas plant pathogens 6 2.1 A core-genome sequence based taxonomy of the family Leptotrichiaceae calculated using EDGAR 2.0 ......................... 7 2.2 A global catalogue of microbial genome: type strain sequencing project of WDCM .................................................... 8 2.3 Genome sequence-based criteria for species demarcation: insights from the genus Rickettsia .............................................. 9 2.4 What exactly are bacterial subspecies? ............................. 10 3.1 Actinobacterial biodiversity: a potential driver for the South African Bio-economy ..................................................... 11 3.2 Identification and recovery of “missing microbes” from the gut microbiota of human populations living non-industrial lifestyles ..................................................................................... -
Stress-Tolerance and Taxonomy of Culturable Bacterial Communities Isolated from a Central Mojave Desert Soil Sample
geosciences Article Stress-Tolerance and Taxonomy of Culturable Bacterial Communities Isolated from a Central Mojave Desert Soil Sample Andrey A. Belov 1,*, Vladimir S. Cheptsov 1,2 , Elena A. Vorobyova 1,2, Natalia A. Manucharova 1 and Zakhar S. Ezhelev 1 1 Soil Science Faculty, Lomonosov Moscow State University, Moscow 119991, Russia; [email protected] (V.S.C.); [email protected] (E.A.V.); [email protected] (N.A.M.); [email protected] (Z.S.E.) 2 Space Research Institute, Russian Academy of Sciences, Moscow 119991, Russia * Correspondence: [email protected]; Tel.: +7-917-584-44-07 Received: 28 February 2019; Accepted: 8 April 2019; Published: 10 April 2019 Abstract: The arid Mojave Desert is one of the most significant terrestrial analogue objects for astrobiological research due to its genesis, mineralogy, and climate. However, the knowledge of culturable bacterial communities found in this extreme ecotope’s soil is yet insufficient. Therefore, our research has been aimed to fulfil this lack of knowledge and improve the understanding of functioning of edaphic bacterial communities of the Central Mojave Desert soil. We characterized aerobic heterotrophic soil bacterial communities of the central region of the Mojave Desert. A high total number of prokaryotic cells and a high proportion of culturable forms in the soil studied were observed. Prevalence of Actinobacteria, Proteobacteria, and Firmicutes was discovered. The dominance of pigmented strains in culturable communities and high proportion of thermotolerant and pH-tolerant bacteria were detected. Resistance to a number of salts, including the ones found in Martian regolith, as well as antibiotic resistance, were also estimated. -
Gordonia Alkanivorans Sp. Nov., Isolated F Rorn Tar-Contaminated Soil
International Journal of Systematic Bacteriology (1999), 49, 1513-1 522 Printed in Great Britain Gordonia alkanivorans sp. nov., isolated f rorn tar-contaminated soil Christei Kummer,’ Peter Schumann’ and Erko Stackebrandt2 Author for correspondence : Christel Kummer. Tel : + 49 36 4 1 65 66 66. Fax : + 49 36 4 1 65 66 52. e-mail : chkummer @ pmail. hki-jena.de ~ Hans-Knoll-lnstitut fur Twelve bacterial strains isolated from tar-contaminated soil were subjected to Naturstoff-Forschunge.V., a polyphasic taxonomic study. The strains possessed meso-diaminopimelicacid D-07745 Jena, Germany as the diagnostic diamino acid of the peptidoglycan, MK-9(H2) as the * DSMZ - Deutsche predominant menaquinone, long-chain mycolic acids of the Gordonia-type, Sammlung von Mikroorganismen und straight-chain saturated and monounsaturated fatty acids, and considerable Zellkulturen GmbH, amounts of tuberculostearic acid. The G+C content of the DNA was 68 molo/o. D-38124 Braunschweig, Chemotaxonomic and physiologicalproperties and 165 rDNA sequence Germany comparison results indicated that these strains represent a new species of the genus Gordonia. Because of the ability of these strains to use alkanes as a carbon source, the name Gordonia alkanivorans is proposed. The type strain of Gordonia alkanivorans sp. nov. is strain HKI 0136T(= DSM 4436gT). Keywords: Actinomycetales, Gordonia alkanivorans sp. nov., tar-contaminated soil, degradation, alkanes INTRODUCTION al., 1988). The genus was emended (Stackebrandt et al., 1988) to accommodate Rhodococcus species con- The genus Gordonia belongs to the family taining mycolic acids with 48-66 carbon atoms and Gordoniaceae of the suborder Corynebacterineae MK-9(H2) as the predominant menaquinone. Rhodo- within the order Actinomycetales (Stackebrandt et al., coccus aichiensis and Nocardia amarae were reclassified 1997). -
Isolation of Arthrobacter Species from the Phyllosphere and Demonstration of Their Epiphytic fitness Tanja R
ORIGINAL RESEARCH Isolation of Arthrobacter species from the phyllosphere and demonstration of their epiphytic fitness Tanja R. Scheublin1 & Johan H. J. Leveau1,2 1Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, Wageningen, 6708 PB, The Netherlands 2Department of Plant Pathology, University of California, One Shields Avenue, Davis, California 95616 Keywords Abstract Arthrobacter, biodegradation, phylloplane, phylloremediation, soil, triadimenol Bacteria of the genus Arthrobacter are common inhabitants of the soil environ- ment, but can also be recovered from leaf surfaces (the phyllosphere). Using Correspondence enrichment cultures on 4-chlorophenol, we succeeded in specifically isolating Johan Leveau, University of California, Arthrobacter bacteria from ground cover vegetation in an apple orchard. Based Department of Plant Pathology, One Shields on 16S rRNA gene sequencing, the isolates were found to belong to at least Avenue, Davis, CA 95616. three different species of Arthrobacter. Compared to the model bacterial epi- Tel: +1 (530) 752 5046; Fax: +1 (530) 752 5674; E-mail: [email protected] phyte Pantoea agglomerans, the Arthrobacter isolates performed as well or even better in a standardized laboratory test of phyllosphere fitness. A similar perfor- Funding Information mance was observed with the well-characterized soil isolate Arthrobacter chloro- This work was supported as part of the phenolicus A6. These findings suggest that the frequently reported presence of BACSIN project within -
Gordonia Polyisoprenivorans from Groundwater
Brazilian Journal of Microbiology (2006) 37:168-174 ISSN 1517-8382 GORDONIA POLYISOPRENIVORANS FROM GROUNDWATER CONTAMINATED WITH LANDFILL LEACHATE IN A SUBTROPICAL AREA: CHARACTERIZATION OF THE ISOLATE AND EXOPOLYSACCHARIDE PRODUCTION Roberta Fusconi1*; Mirna Januária Leal Godinho1; Isara Lourdes Cruz Hernández1; Nelma Regina Segnini Bossolan2 1Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos, São Carlos, SP, Brasil; 2Instituto de Física, Universidade de São Paulo, São Carlos, SP, Brasil Submitted: April 30, 2004; Returned to authors for corrections: June 01, 2005; Approved: February 26, 2006 ABSTRACT A strain of Gordonia sp. (strain Lc), from landfill leachate-contaminated groundwater was characterized by polyphasic taxonomy and studied for exopolysaccharide (EPS) production. The cells were Gram-positive, catalase-positive, oxidase-negative and non-motile. The organism grew both aerobically and, in anoxic environment, in the presence of NaNO3. Rods occured singly, in pairs or in a typical coryneform V-shaped. The organism had morphological, physiological and chemical properties consistent with its assignment to the genus Gordonia and mycolic and fatty acid pattern that corresponded to those of G. polyisoprenivorans DSM 44302T. The comparison of the sequence of the first 500 bases of the 16S rDNA of strain Lc gave 100% similarity with the type strain of Gordonia polyisoprenivorans DSM 44302T. Experiments conducted in anaerobic conditions in liquid E medium with either glucose or sucrose as the main carbon source showed that sucrose did not support the growth of Lc strain and that on glucose the maximum specific growth rate was 0.17h-1, representing a generation time of approximately 4 hours. On glucose, a maximum of total EPS was produced during the exponential phase (126.17 ± 15.63 g l-1). -
Investigation of New Actinobacteria for the Biodesulphurisation of Diesel Fuel
Investigation of new actinobacteria for the biodesulphurisation of diesel fuel Selva Manikandan Athi Narayanan A thesis submitted in partial fulfilment of the requirements of Edinburgh Napier University, for the award of Doctor of Philosophy May 2020 Abstract Biodesulphurisation (BDS) is an emerging technology that utilises microorganisms for the removal of sulphur from fossil fuels. Commercial-scale BDS needs the development of highly active bacterial strains which allow easier downstream processing. In this research, a collection of actinobacteria that originated from oil-contaminated soils in Russia were investigated to establish their phylogenetic positions and biodesulphurisation capabilities. The eleven test strains were confirmed as members of the genus Rhodococcus based on 16S rRNA and gyrB gene sequence analysis. Two organisms namely strain F and IEGM 248, confirmed as members of the species R. qingshengii and R. opacus, respectively based on the whole- genome sequence based OrthoANIu values, exhibited robust biodesulphurisation of dibenzothiophene (DBT) and benzothiophene (BT), respectively. R. qingshengii strain F was found to convert DBT to hydroxybiphenyl (2-HBP) with DBTO and DBTO2 as intermediates. The DBT desulphurisation genes of strain F occur as a cluster and share high sequence similarity with the dsz operon of R. erythropolis IGTS8. Rhodococcus opacus IEGM 248 could convert BT into benzofuran. The BDS reaction of both strains follows the well-known 4S pathway of desulphurisation of DBT and BT. When cultured directly in a biphasic growth medium containing 10% (v/v) oil (n-hexadecane or diesel) containing 300 ppm sulphur, strain F formed a stable oil-liquid emulsion, making it unsuitable for direct industrial application despite the strong desulphurisation activity. -
Characterization of Actinobacteria Degrading and Tolerating Organic Pollutants and Tolerating Organic Pollutants
Characterization of Actinobacteria Degrading Characterization of Actinobacteria Degrading and Tolerating Organic Pollutants and Tolerating Organic Pollutants Irina Tsitko Irina Tsitko Division of Microbiology Division of Microbiology Department of Applied Chemistry and Microbiology Department of Applied Chemistry and Microbiology Faculty of Agriculture and Forestry Faculty of Agriculture and Forestry University of Helsinki University of Helsinki Academic Dissertation in Microbiology Academic Dissertation in Microbiology To be presented, with the permission of the Faculty of Agriculture and Forestry of the To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public criticism in Auditorium 1015 at Viikki Biocentre, University of Helsinki, for public criticism in Auditorium 1015 at Viikki Biocentre, Viikinkaari 5, on January 12th, 2007, at 12 o’clock noon. Viikinkaari 5, on January 12th, 2007, at 12 o’clock noon. Helsinki 2007 Helsinki 2007 Supervisor: Professor Mirja Salkinoja-Salonen Supervisor: Professor Mirja Salkinoja-Salonen Department of Applied Chemistry and Microbiology Department of Applied Chemistry and Microbiology University of Helsinki University of Helsinki Helsinki, Finland Helsinki, Finland Reviewers Doctor Anja Veijanen Reviewers Doctor Anja Veijanen Department of Biological and Environmental Science Department of Biological and Environmental Science University of Jyväskylä University of Jyväskylä Jyväskylä, Finland Jyväskylä, Finland Docent Merja Kontro Docent Merja Kontro University of Helsinki University of Helsinki Department of Ecological and Environmental Sciences Department of Ecological and Environmental Sciences Lahti, Finland Lahti, Finland Opponent: Professor Edward R.B. Moore, Opponent: Professor Edward R.B. Moore, Department of Clinical Bacteriology Department of Clinical Bacteriology Sahlgrenska University Hospital, Sahlgrenska University Hospital, Göteborg University Göteborg University Göteborg, Sweden. -
Universidad Politécnica De Valencia
UNIVERSIDAD POLITÉCNICA DE VALENCIA ESCUELA TÉCNICA SUPERIOR DE INGENIEROS AGRÓNOMOS DIVERSIDAD DE ACTINOMICETOS NOCARDIOFORMES PRODUCTORES DE ESPUMAS BIOLÓGICAS AISLADOS DE PLANTAS DEPURADORAS DE AGUAS RESIDUALES DE LA COMUNIDAD VALENCIANA TRABAJO FINAL DE CARRERA INGENIERO AGRÓNOMO PRESENTADO POR: ALBERT SOLER HERNÁNDEZ DIRIGIDO POR: GONZALO CUESTA AMAT JOSE LUIS ALONSO MOLINA VALENCIA, JULIO DE 2008 ESCUELA TÉCNICA Anexo 4 SUPERIOR DE INGENIEROS Autorización del director/a, AGRÓNOMOS codirector/a o tutor/a Datos del trabajo de fin de carrera Autor: Albert Soler Hernández DNI: 22584259-F Título: DIVERSIDAD DE ACTINOMICETOS NOCARDIOFORMES PRODUCTORES DE ESPUMAS BIOLÓGICAS AISLADOS DE PLANTAS DEPURADORAS DE AGUAS RESIDUALES DE LA COMUNIDAD VALENCIANA Área o áreas de conocimiento a las que corresponde el trabajo: Microbiología y Medio Ambiente Titulación: Ingeniero Agrónomo A cumplimentar por el director/a, codirector/a o tutor/a del trabajo Nombre y apellidos: Gonzalo Cuesta Amat Departamento: Biotecnología En calidad de: X director/a codirector/a tutor/a Autorizo la presentación del trabajo de fin de carrera cuyos datos figuran en el apartado anterior y certifico que se adecua plenamente a los requisitos formales, metodológicos y de contenido exigidos a un trabajo de fin de carrera, de acuerdo con la normativa aplicable en la ETSIA. (Firma)* Gonzalo Cuesta Amat Jose Luis Alonso Molina Valencia, 21 de Julio de 2008 En el caso de codirección, han de firmar necesariamente los que sean profesores de esta Escuela; si existe tutor o tutora, -
Actividad Formadora De Canales Transmembrana En La Superficie De Gordonia Jacobaea
Actividad formadora de canales transm embrana en la superficie de Gordonia jacobaea Mª Guadalupe Jiménez Galisteo ADVERTIMENT . La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX ( www.tdx.cat ) i a través del Dipòsit Digital de la UB ( diposit.ub.edu ) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposici ó des d’un lloc aliè al servei TDX ni al Dipòsit Digital de la UB . No s’autoritza la presentació del seu contingut en un a finestra o marc aliè a TDX o al Dipòsit Digital de la UB (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona auto ra. ADVERTENCIA . La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR ( www.tdx.cat ) y a través del Repositorio Digital de la UB ( diposit.ub.edu ) ha sido au torizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR o al Repositorio Digital de la UB . -
Arthrobacter Pokkalii Sp Nov, a Novel Plant Associated Actinobacterium with Plant Beneficial Properties, Isolated from Saline Tolerant Pokkali Rice, Kerala, India
RESEARCH ARTICLE Arthrobacter pokkalii sp nov, a Novel Plant Associated Actinobacterium with Plant Beneficial Properties, Isolated from Saline Tolerant Pokkali Rice, Kerala, India Ramya Krishnan1¤, Rahul Ravikumar Menon1, Naoto Tanaka2, Hans-Jürgen Busse3, Srinivasan Krishnamurthi4, Natarajan Rameshkumar1¤* 1 Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India, 2 NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156–8502, Japan, 3 Institute of Microbiology, Veterinary University Vienna, A-1210, Vienna, Austria, 4 Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sec-39A, Chandigarh, 160036, India ¤ Current address: Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 001, India * [email protected] OPEN ACCESS Citation: Krishnan R, Menon RR, Tanaka N, Busse Abstract H-J, Krishnamurthi S, Rameshkumar N (2016) Arthrobacter pokkalii sp nov, a Novel Plant A novel yellow colony-forming bacterium, strain P3B162T was isolated from the pokkali rice Associated Actinobacterium with Plant Beneficial rhizosphere from Kerala, India, as part of a project study aimed at isolating plant growth Properties, Isolated from Saline Tolerant Pokkali Rice, Kerala, India. PLoS ONE 11(3): e0150322. beneficial rhizobacteria from saline tolerant pokkali rice and functionally evaluate their abili- doi:10.1371/journal.pone.0150322 ties to promote plant growth -
Aix Marseille Université Ecole Doctorale Des Sciences De La Vie Et De La Santé
Aix Marseille Université Ecole Doctorale des Sciences de la Vie et de la Santé THESE Pour l’obtention du grade de Docteur d’Aix Marseille Université en Microbiologie Thèse présentée et soutenue par Nicolas CHABERT Le 21 décembre 2017 Sous la direction de Dr. ACHOUAK Wafa (Directrice de recherche, UMR 7265 CNRS-CEA-AMU) Conception de biocathode et implication du fer dans de nouveaux modes de transfert d’électrons Rapporteurs Dr. BERGEL Alain (Directeur de recherche, CNRS) Dr. BOUSSERRHINE Noureddine (Maitre de conférence, UPEC) Examinateurs Dr. LOJOU Elisabeth (Directeur de recherche, CNRS) Dr. HAICHAR Feteh-El-Zahar (Maitre de conférence, Université de Lyon 1) Pr. CUNY Philippe (Professeur, Aix Marseille Université) Invités Dr. BONNEFOY Violaine (Directeur de recherche, CNRS) Dr. ROSE Jérôme (Directeur de recherche, CNRS) Résumé : Les piles à combustible microbiennes (PCMs) sont une technologie convertissant l’énergie chimique stockée dans la matière organique en énergie électrique à l’anode à l’aide de bactéries dites électroactives. Actuellement, les performances sont limitées par l’utilisation de cathodes abiotiques. L’intérêt porté aux cathodes biologiques est récent et ces dernières sont encore peu étudiées. Les objectifs de ces recherches ont donc été d’identifier et de décrire des bactéries ainsi que les mécanismes permettant de catalyser une réduction cathodique notamment celle de l’oxygène. La première partie des travaux portant sur la réalisation d’un criblage pour la formation de biocathodes a révélé l’inefficacité du métabolisme hétérotrophe pour la conception de biocathodes performantes. De ce fait, la suite des travaux a porté sur la bactérie chimiolithoautotrophe acidophile Acidithiobacillus ferrooxidans.