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ORAL PRESENTATIONS INDEX 1.1 Revisiting genotypic and phenotypic properties as an aid to circumscribe species of the genus Salinispora .......................... 1 1.2 The use of whole genome sequencing to confirm the recognition of M. noduli and M. saelicesensis ........................... 2 1.3 Genome-scale data call for a taxonomic rearrangement of Geodermatophilaceae ................................................................... 3 1.4 Diversity and distribution of sphingomonads ................... 4 1.5 Genome-informed Bradyrhizobium taxonomy: where to from here? .................................................................................... 5 1.6 Divergence and gene flow in Xanthomonas plant pathogens 6 2.1 A core-genome sequence based taxonomy of the family Leptotrichiaceae calculated using EDGAR 2.0 ......................... 7 2.2 A global catalogue of microbial genome: type strain sequencing project of WDCM .................................................... 8 2.3 Genome sequence-based criteria for species demarcation: insights from the genus Rickettsia .............................................. 9 2.4 What exactly are bacterial subspecies? ............................. 10 3.1 Actinobacterial biodiversity: a potential driver for the South African Bio-economy ..................................................... 11 3.2 Identification and recovery of “missing microbes” from the gut microbiota of human populations living non-industrial lifestyles ...................................................................................... 12 3.3 Capturing the hidden bacterial diversity at the Lonar Crater, India formed ~52000 years ago .................................................. 13 3.4 Phylogenetic characterisation of Streptomyces species causing fissure scab of potatoes in South Africa .................... 14 4.1 Genomic diversity of human gut-associated Treponema inferred from shotgun metagenomic datasets ........................ 15 4.2 Application of whole genome sequencing to exploring intra- generic heterogeneity ................................................................ 16 4.3 Relating tRNA gene diversity with the evolution of Planctomycetes-Verrucomicrobia-Chlamydia (PVC) and PVC-like bacteria. ..................................................................... 17 4.4 Phylogenomic analyses: The whole is greater than the sum of its parts .................................................................................. 18 5.1 Bacterial species are sui generis evolutionary units ......... 19 5.2 Multiple uses of genome sequences in characterizing novel bacteria. ..................................................................................... 20 5.3 Importance of microbial culture collections (mBRC’s) .. 21 POSTER PRESENTATIONS.................................................... 1 B1 Trusting phylogeny or chemotaxonomy? A case study for Turicella otitidis including Corynebacterium otitidis comb. nov… .......................................................................................... 23 B2 An EzBioCloud based k-mer database for an accurate metagenomic taxonomy classification ..................................... 24 B4 KI-S: a tool for very fast taxonomic comparison of genomic sequences .................................................................... 25 B5 Developing a molecular consensus between multiple phylogenies: A case study of the genus Aeromonas ............... 26 B8 Culture dependent and culture independent studies on Socorro island (Revillagigedo archipelago) suggests an intrinsic diversity of actinobacterial species ........................... 27 B9 A novel soil bacterium Hymenobacter humicola sp. nov. 3isolated in Antarctica .............................................................. 28 B10 Genomic encyclopedia of bacterial and archaeal type strains: the genomes and pangenomes of soil and plant- associated prokaryotes and newly described type strains ..... 29 B11 Diverse alpha- and beta-rhizobia nodulate Vachellia karroo in South Africa .............................................................. 30 B12 The genetic characterization of Streptomyces isolates causing fissure scab on potatoes in the Limpopo Province ... 32 B13 Morphological characterisation of Streptomyces species associated with fissure scab of potatoes in South Africa ....... 33 B14 Micromonospora species isolated from high altitude Atacama Desert soils ................................................................. 34 B15 Diversity of Actinobacteria in Indonesian arid habitats as a source of novel antimicrobial drug leads ............................. 35 B17 Optimization of motoho, a fermented sorghum beverage from Southern Africa ................................................................ 37 B19 Diversity and characterization of staphylococci associated with animals from Antarctica .................................................. 38 B20 Study of microbial contamination in meat sold in butcheries and supermarkets around Mafikeng, North West Province ...................................................................................... 39 B22 Studies on the efficient degradation of phthalates by Gordonia sp. YC-RL2 and Mycobacterium sp. YC-RL4 ...... 40 Oral Presentations 1.1 Revisiting genotypic and phenotypic properties as an aid to circumscribe species of the genus Salinispora LA Maldonado1, L Contreras-Castro2, JA Acevedo-Becerra2, ME Esteva- García2, JC Cancino-Díaz2, CJ Hernández-Guerrero3, S Martínez-Díaz3, ET Quintana2 1 Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico 2 Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Mexico City, Mexico 3 Centro Interdisciplinario de Ciencias Marinas (CICIMAR), IPN, La Paz, Mexico The genus Salinispora Maldonado et al. 2005 was originally described to accommodate two actinobacterial species, namely S. arenicola and S. tropica recovered from marine sediments. A third species, S. pacifica, was described later (2013) and the genus solely encompasses marine obligate actinobacterial species of biotechnological importance. Description of novel salinisporae is difficult mainly due to the high level of similarity found between the three species at the 16S rRNA gene level, thus, alternative genes are needed to properly circumscribe and facilitate the description of putative novel candidates. Although there are phenotypic properties which helps to differentiate between the three species, genotypic data has contributed heavily to the circumscription of salinisporae. It is important to properly separate between the three species due to their secondary metabolism: S. arenicola produces ansamycins, S. pacifica cyanosporoside A and S. tropica salinisporamide A, to name a few. In this work, a collection of 50 salinisporae were recovered from the Sea of Cortez and their taxonomic position within the validly described species of the genus compared by using a collection of geno- and phenotypic properties. All of the isolates were found to be related to S. arenicola but some formed a distinct subclade after atpD, gyrB, rpoB, secY, and 16S gene analyses. A comparison of the sequences of the rifK gene also confirmed the separation of some of our isolates to S. arenicola hence suggesting (at least) a fourth species within the genus though genome sequencing plus ANI comparisons may also contribute to resolve the intrinsic relationship within the isolates and to S. arenicola. Regarding secondary metabolite production, the isolates show in vitro antibiotic activity against clinical isolates of Staphylococcus aureus and Vibrio parahaemolyticus thus enhancing the bioprospecting importance of our putative novel species. 1 1.2 The use of whole genome sequencing to confirm the recognition of M. noduli and M. saelicesensis R. Riesco1, C. Prieto2, M. E. Trujillo1 1 Departamento de Microbiología y Genética, Edificio Departamental, Universidad de Salamanca, Salamanca, Spain. [email protected] 2 Servicio de Bioinformática, NUCLEUS, Edificio I+D+I, Universidad de Salamanca Micromonospora noduli, isolated from a nitrogen fixing nodule of Pisum sativum was formally described in 2016. The genomic delimitation of this species was based on 16S rRNA gene phylogeny, five partial core gene multilocus sequence analysis (MLSA) and DNA-DNA hybridization (DDH). The latter test showed that M. saelicesensis, also isolated from a nodule tissue, but in this case of the legume Lupins angustifoius, was the closest species, sharing a DDH value of 62.8% (63.4%). As part of a comparative genomics study of the species Micromonospora saelicesensis, ten strains that had a 16S rRNA gene identity above 99.5% with the type strain, were selected and their genomes sequenced. As previous analyses based on 16S rRNA gene phylogeny and MLSA also suggested that some of these strains were closely related to the species M. noduli, the genome of the type strain, GUI43T, was also sequenced for comparison. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses, with values just above the recommended threshold for the delimitation of species (96.64% and 71.20% respectively), highlighted the close relationship between M. saelicensesis and M. noduli type strains, questioning the species description of the latter one 2016. Phylogenomic analyses using whole-genome sequences of the 10 strains, separated them in two very closely related groups, one including the type strain of M. saelisecensis (ANI values ≥97.8% and