RESEARCH ARTICLE Ectomycorrhizal fungal communities in endangered Pinus amamiana forests Masao Murata1*, Seiichi Kanetani2, Kazuhide Nara1 1 Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan, 2 Kyushu Research Center, Forestry and Forest Products Research Institute, Chuo-ku, Kumamoto, Japan *
[email protected] a1111111111 a1111111111 Abstract a1111111111 a1111111111 Interactions between trees and ectomycorrhizal (ECM) fungi are critical for the growth and a1111111111 survival of both partners. However, ECM symbiosis in endangered trees has hardly been explored, complicating conservation efforts. Here, we evaluated resident ECM roots and soil spore banks of ECM fungi from endangered Pinus amamiana forests on Yakushima and Tanegashima Islands, Kagoshima Prefecture, Japan. Soil samples were collected OPEN ACCESS from remaining four forests in the two islands. The resident ECM roots in soil samples were subjected to molecular identification. Soil spore banks of ECM fungi were analyzed via bio- Citation: Murata M, Kanetani S, Nara K (2017) Ectomycorrhizal fungal communities in assays using a range of host seedlings (P. amamiana, P. parviflora, P. densiflora and Casta- endangered Pinus amamiana forests. PLoS ONE nopsis sieboldii) for 6±8 months. In all remaining P. amamiana forests, we discovered a new 12(12): e0189957. https://doi.org/10.1371/journal. Rhizopogon species (Rhizopogon sp.1), the sequence of which has no match amoung pone.0189957 numerous Rhizopogon sequences deposited in the international sequence database. Host Editor: Erika Kothe, Friedrich Schiller University, identification of the resident ECM roots confirmed that Rhizopogon sp.1 was associated GERMANY only with P. amamiana. Rhizopogon sp.1 was far more dominant in soil spore banks than in Received: August 27, 2017 resident ECM roots, and its presence was confirmed in nearly all soil samples examined Accepted: December 5, 2017 across the major remaining populations.