Effect of Seed Storage and Seed Treatments on Seedling Growth of Garcinia Gummi-Gutta (L.) Roxb
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Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 06 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.706.456 Effect of Seed Storage and Seed Treatments on Seedling Growth of Garcinia gummi-gutta (L.) Roxb. in Ponnampet, Western Ghat Aditya Kumar Jayant1, N.M. Poonacha1, G.M. Devagiri1, Vijay Kumar Yadav2* and P.S. Chauhan3 1College of Forestry Ponnampet, University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka 577204, India 2Department of Forestry and Natural Resources, HNB Garhwal University, Srinagar- Garhwal, Uttarakhand 246174, India 3College of Horticulture and Forestry, Jhalrapatan, Jhalawar, Agriculture University, Kota-326 023, India *Corresponding author ABSTRACT K e yw or ds The experiment was conducted to examine the effect of seed storage and seed treatments on seedling growth of Garcinia gummi-gutta at College of forestry, Ponnampet in split Garcinia gummi-gutta, plot design in that seed storage selected as main plot and seed treatment as sub plot. In the Growth , Seedling, Seed storage, Seed present study seed stored up to 0, 30, 60, 90 days respectively with the eight different seed treatment treatments. In each treatment 30 seeds were used per replication. The study indicated that seed stored up to 6o days has significant effect on growth. Results indicates that seed Article Info treated with mechanical scarification of seed coat showed best growth while seed treated Accepted: with hydrogen peroxide and hot water treatment showed less growth. The overall result 30 April 2018 concluded that seed stored up to 60 days and seed treated with mechanical scarification of Available Online: seed coat found best for the seedling growth of Garcinia gummi-gutta. 10 June 2018 Introduction bark with an average thickness of 5.3 mm (Hegde et al., 1998). These trees grow up to Genus Garcinia belongs to the family an average height of 18 m and attain diameter Clusiaceae (Guttiferrae). It is composed of of up to 70 cm. Gacinia gummi-gutta trees are approximately 400-600 species, including dioecious, with a male to female sex ratio of dioecious evergreen trees growing in the 1:1. Trees of both the sexes usually commence tropical parts of the world (Steentolft, 1988; flower production when they are about 14 cm Richards, 1990). Garcinia gummi-gutta (L.) in diameter. Male and female trees produce Roxb. is a moderate sized tree with round flowers from early February to April. Fruits canopy, drooping branches (Shringeshwara et ripen from June to August. Fruit is an al., 2000) and the trees have a dark, smooth indehiscent berry, pulp juicy and weigh about 3874 Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 80 g (Saldanha, 1984). The English name of and high yielding mother plants from nearby the tree is Malabar Gamboge. In Kannada it is estates in Ponnampet, Kodagu during month known as Simai Hunase, and Uppage of July. Seeds were extracted from the fruit of (Saldanha, 1984). The scientific name of the Garinia gummi-gutta and wash thoroughly to species is derived from a botanist Garcinia remove pulp. The experiment was conducted who described it in 1547 (Subhas, 1996). in split plot design with four replications in Garcinia gummi-gutta is also known as that seed storage selected as main plot and Garcinia cambogia, probably got its name seed treatment as sub plot. In the present study from the gum tree gummi-gutta or gamboges seed stored up to 0, 30, 60, 90 days used as pigment in miniature paintings and respectively with the eight different seed water colors (Bhandari, 1998). treatments. In each treatment 30 seeds were used per replication. Observation on growth Garcinia gummi-gutta, is a common medicinal recorded after 180 day of sowing for each plant, has been used historically to treat storage period. The data collected was respiratory infections such as sore throat and subjected to analysis of variance (adjusted for cough. The phyto-chemical constituents covariate) based on Split plot design using the include bi-flavinoid, xanthone and GENSTAT software (Genstat 5 Release 3.2) benzophenones and principle acid in the fruit after angular transformation of per cent values and rind is Hydroxycitric Acid (HCA) and square root transformation of count. The (Jayaprakash and Sakariah, 1998). The acid covariates considered for the analysis of has been found to suppress fatty acid covariance is Air temperature. synthesis, lipogenesis, and food intake and to promote glycogenesis, while inducing weight Treatment details loss (Jena et al., 2002). The sun dried rind of the fruit is astringent, antiseptic and purgative. Main plot- storage Due to its commercial importance several M1: Sowing of fresh seeds. farmers of the Western Ghats have shown M2: Sowing after 30 days storage. interest in bringing the species under M3: Sowing after 60 days storage. cultivation (Vasudeva et al., 2003). M4: Sowing after 90 days storage. Owing to these important properties the Sub plot - Seed treatments demand for the fruit has increased, which in S1 Control (soaking in water) turn has led to the unscientific and reckless S2 Mechanical damaging of seed coat exploitation. In rural areas of the Western S3 Acid scarification for 3 minute Ghats, people harvest the fruits even when S4 Treatment of kernel with 50 ppm GA3 they are green and there are also instances of after removal of seed coat severe loping of branches for easy collection S5 Cow dung slurry treatment for 48 of fruits. As a result, the species has already hours been declared as “threatened” as per the IUCN S6 Hot water treatment for 30 minutes guidelines (Ravi Kumar and Ved, 2000; S7 H2O2 (30%) treatment for 30 minutes Ramesh et al., 1997). S8 Fresh cow urine treatment for 24 hour Materials and Methods Observations recorded The present study was conducted at college of Root and shoot length (cm): Root and shoot forestry, Ponnampet, Kodagu district, length of seedlings was taken by the help of Karnataka. Fruits were collected from healthy scale after 180 days of sowing, the seedlings 3875 Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 were carefully uprooted with least disturbance Results and Discussion to the root from the soil. Root portion was washed to remove the soil. Shoot length was Effect of storage on seedling growth measured from collar region to the top most nodal region. The root length was measured The study revealed that the effect of seed from collar region to the tip of the longest storage on seed germination showed root. For each treatment one seedling was significant differences for growth parameters taken. except root length. Out of the different storage period tried, seed stored up to 60 days after Collar diameter (cm): Collar diameter of collection recorded the higher collar diameter, seedling was taken by using digital calliper. average number of leaves, root length and shoot length while the shoot weight and root Average Number of leaves: Leaves were weight were found maximum in seed stored counted after 180 days of sowing. up to 90 days after collection of seed. The minimum growth parameter were observed in Root and shoot biomass (g/plant): Dry weight fresh seeds except collar diameter which is of root and shoot were taken by harvesting one found minimum in seed stored up to 90 days plant from each of the replication for storage (Table 1). and seed treatment by keeping in hot air oven at 70˚ C for 72 hours. Table.1 Effect of seed storage on seedling growth parameters Storage Collar Average Root Shoot Root Shoot diameter number of length length (cm) weight weight (mm) leaves per (cm) (g) (g) plant b a a a a M1 2.06 8.80 13.92 11.67 0.61 0.93 (2.97)* c b b b ab M2 2.21 10.73 15.15 12.60 0.71 1.06 (3.28) c c b c b M3 2.25 11.92 15.71 13.44 0.75 1.12 (3.45) a a b d c M4 1.84 9.01 14.69 12.71 1.15 1.53 (3.01) Mean 2.09 10.08 14.86 12.60 0.80 0.60 (3.18) SEm± 0.04 0.09 0.47 0.27 0.03 0.13 LSD(0.05) 0.15 0.30 NS 0.92 0.09 0.17 *Values in Parentheses are square root transformed; LSD- Least Significant Difference NS- Non Significant, Figure with similar letters as superscript do not differ significantly 3876 Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 Table.2 Effect of seed treatments on seedling growth parameters Treatments Collar Average number Root Shoot Root Shoot diameter of leaves per length length weight weight (mm) plant (cm) (cm) (g) (g) c d b c c b S1 2.38 12.55 (3.54)* 16.62 13.21 0.74 1.34 c e bc d e c S2 2.63 13.68 (3.70) 17.37 15.58 1.23 1.77 b b a b bc a S3 1.66 8.68 (2.95) 10.25 10.71 0.57 0.59 c c c d e c S4 2.53 12.04 (3.47) 19.33 17.37 1.22 1.70 c c bc c d bc S5 2.41 12.22 (3.50) 18.62 13.63 0.93 1.53 a a a a ab a S6 1.29 5.16 (2.27) 8.92 8.54 0.40 0.40 ab a a a a a S7 1.42 4.89 (2.21) 10.58 8.46 0.36 0.31 c c b c d bc S8 2.40 12.17 (3.49) 17.21 13.33 0.97 1.60 Mean 2.09 10.8(2.19) 14.86 12.60 0.80 1.16 SEm± 0.10 0.10 0.73 0.65 0.06 0.12 LSD(0.05) 0.29 0.29 2.07 1.84 0.18 0.34 *Values in Parentheses are square root transformed; LSD- Least Significant Difference, Figure with similar letters as superscript do not differ significantly Fig.1 Effect of different treatments on germination per cent Plate.1 Influence of seed treatments on seedling growth in fresh seeds 3877 Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 Plate 2: Influence of seed treatments on seedling growth in seed stored for 30 days Plate 3: Influence of seed treatments on seedling growth in seed stored for 60 days Plate 4: Influence of seed treatments on seedling growth in seed stored for 90 days 3878 Int.J.Curr.Microbiol.App.Sci (2018) 7(6): 3874-3880 Effect of seed treatment on seedling growth method for breaking seed coat dormancy.