DOCSLIB.ORG

Screening Environmental Pseudomonads for Characteristics

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Screening Environmental Pseudomonads for Characteristics Screening Environmental Pseudomonads for Characteristics Suitable for a Bio-engineered Oil Remediation Agent By Mohammed Ibrahim Umar B Eng., MSc A thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy (PhD) in the School of Science, Engineering and Technology, Abertay University Dundee, United Kingdom April, 2016 DEDICATION This dissertation work is dedicated to my loving and caring children for given me joy when am bored writing and resuscitating me back on track; Ummu-salma (Mami), Umar-Farouk (Amir), Halimatu-sadiya, Abubakar-sadiq (Bappa/Sarki), Sumayya, Ibrahim (Mallam), Fatimatu-zahra’u, and Muhammad Page ii AUTHOR’S DECLARATION I, Ibrahim Umar Mohammed hereby wilfully declare that the following thesis is based on the results of research wholly conducted by myself, and that it is of my own composition. This thesis has not, in whole or in part, been previously presented for a higher degree or qualification. All works used in the course of this work are evidently referenced as appropriate. …………………………….... Date: 27-04-2016 School of Science, Engineering & Technology (SET), Abertay University Dundee DD1 1HG, United Kingdom Page iii CERTIFICATION I hereby certify that Ibrahim Umar Mohammed, a PhD candidate has undertaken all the work described herein and is based on the original work done at the Abertay University, Dundee in partial fulfilment for the requirements for the award of Doctor of Philosophy in Environmental Bioremediation. This has not previously formed the basis for the award of any degree, diploma, assistanceship, fellowship or any similar title and represents an independent work on the part of the candidate. Signature of the Principal Supervisor…………………………….... Dr. Andrew Spiers, School of Science, Engineering & Technology (SET), Abertay University Dundee DD1 1HG, United Kingdom Page iv ACKNOWLEDGEMENT I would like to thank my PhD supervisors, Dr Andrew Spiers and Dr Yusuf Deeni, for supporting me during these past four years. Andrew has refined my research capabilities through patience, understanding through his immense knowledge. His guidance helped me in all the time of research and writing of this thesis. I hope that I could be as active, enthusiastic and robust as Andrew and to someday be able to use my wealth of knowledge and experience to sway an audience as well as he can. Good supervisor who always encouraged and expected the research group working in the laboratory to think more independently about their experiments and results. Yusuf has been supportive and has given me constructive advice and insightful discussions in all the stages of this work. My appreciation goes to members of the SIMBIOS group, and the entire PhD students of School of Science, Engineering and Technology, who were not part of the group but helped me in one way or the other. I would like to thank my family and parents, dad-late Alh Muhammadu Umaru, mum-Hajiya Salamatu and Hajiya Jummai Umaru for giving birth to me in the first place and supporting me spiritually throughout my life. My brothers and sisters Engr. Muhammad Kabir Umar, Maryam Mamman and Habibu Umar and others too many to mention here for the role you all played. I am indebted to Dr Labaran Lawal & family for their kindness, care and supportive role throughout my stay in Dundee. Page v Thanks also go out to Dr. Simona M. Hapca who provided me with statistical analysis and advice at times of critical need. My friends and colleagues at home, Dr Idris M. Jega, Engr Sirajo Muhammad, Abu Sabo (Small Z) Mu’azu Guraguri, Abdulwahab Garba, Aminu Ja’afar, Engr Ayuba Ndahi, Jamil Hayatu, Hassan Salisu Ahmed, Dr Martin O. Eduvie and others too many to list here but you know who you are, for providing support and friendship that I needed. Bilal Tijjani Kumalia, Sen. Muhammad Ali Ndume for the crucial role you played. Last but not the least; I would like to express my sincere gratitude to Zuzana Spiers for water sample collection and for making my stay in Dundee a memorable one on so many occasions. In conclusion, I recognize that this research would not have been possible without the full sponsorship from The Petroleum Technology Development Fund (PTDF), Nigeria and therefore, my unreserved gratitude and sincere appreciation goes to my beloved country Nigeria. LONG LIVE PTDF AND LONG LIVE FEDERAL REPUBLIC OF NIGERIA. Page vi AUD - PERMISSION TO COPY In submitting this thesis to the Abertay Unversity, Dundee, I understand that I am giving the permission for the research to be made available for use in accordance with the regulations of the University Library for the time being in force, subjected to any copy right vested in the work not being affected thereby. Ibrahim Umar Mohammed (Author) (signed) Date: 27- 04 - 2016 Page vii LIST OF ABBREVIATIONS AND ACRONYMS A – Absorbance AD – Anderson-darling statistics AD* – Adjustted Anderson-darling statistics A–L – Air-liquid interface ANOVA – Analysis of variance Ai – Aqueous index Ei – Emulsion index EPS – Extracellular polymeric substances HCA – Hierarchical cluster analysis IDI – Individual distribution identification LSTRA – Low surface tenstion reducing activity OD – Optical density Oi – Oil index P – Predicted value r – Correlation coefficient SE– Standard error SUDS – Sustainable urban drainage systems TK-HSD – Tukey Kramer Honest Significant Difference ULO – Used lubricating oil γMin – Minimum surface tension Page viii TABLE OF CONTENTS DEDICATION..................................................................................................................ii AUTHOR’S DECLARATION...………………………………………………………………iii CERTIFICATION............…………………………………………………………………......iv ACKOWLEDGEMENT……………………………………………………………………..v-vi UAD – PERMISSION TO COPY…………………………………………………...............vii LIST OF ABBREVIATIONS AND ACRONYMS....…………………………………...viii TABLE OF CONTENTS…………………………………………………………..ix-xv LIST OF FIGURES………………………………………………………………..........xvi-xvii LIST OF TABLES...……………………………………………………………………xviii-xix ABSTRACT……………………………………………………………………………………xx CHAPTER 1 INTRODUCTION……………………………………………………………1 1.1 An introduction to this thesis……………………………………………………………...1 1.2 The origins of environmental pollution…………………………………………………..3 1.3 Bioremediation of hydrocarbon of hydrocarbon contaminated soils and other environments……………………………………………………………………………………4 1.4 The Involvement of bacteria in bioremediation ………………………………………...9 1.4.1 A brief introduction to bacteria for non-microbiologist……………………………...9 1.4.2 Bacterial biofilms……………………………………………………………………...11 1.4.3 The Pseudomonads………………………………………………………………….13 1.5 Bacterial surfactants……………………………………………………………………..16 1.5.1 A brief introduction to surfactants and biosurfactants for the non-chemist…….16 1.5.2 Types of biosurfactants produced by microorganisms and their uses ………...18 Page ix 1.5.3 Prediction of minimum surface tension achieved by bacteria ………………….22 1.5.4 Biosurfactant-oil behaviour…………………………………………………………23 1.6 Aims of this thesis………………………………………………………………………...25 1.6.1 Study rationale………………………………………………………………………..25 1.6.2 Research objectives for this work…………………………………………………..26 CHAPTER 2 MATERIALS AND METHODS……………………………………………...29 2.1 General Microbiology…………………………………………………………………….29 2.1.1 Media and culturing conditions…………………………………………................29 2.1.2 Growth and other measurements by spectrophotometry………………………..30 2.1.3 Bacterial strains used in this work………………………………………………….31 2.2 Sample collection…………………………………………………………………………31 2.2.1 Wastewater…………………………………………………………………………...31 2.2.2 Soil…………………………………………………………………………………….32 2.3 Isolation of pseudomonads……………………………………………………………...32 2.4 Phenotypic characterisation……………………………………………………………..33 2.4.1 Acid production in the presence of maltose……………………………………….33 2.4.2 Catalyse test………………………………………………………………………….34 2.4.3 Drop collapse assay………………………………………………………...............34 2.4.4 Gelatinase activity…………………………………………………………..............34 2.4.5 Kanamycin resistance……………………………………………………………….35 2.4.6 KB culture acidity…………………………………………………………………….35 2.4.7 Lipase activity…………………………………………………………….................35 2.4.8 Mercury resistance…………………………………………………………………..36 Page x 2.4.9 Oxidase test…………………………………………………………………………..36 2.4.10 Protease secretion………………………………………………………………….36 2.4.11 Sodium chloride tolerance………………………………………………..............37 2.4.12 Swarming motility…………………………………………………………………..37 2.4.13 Sugar utilisation…………………………………………………………………….37 2.4.14 Swimming motility…………………………………………………………………..38 2.4.15 Tetracycline resistance…………………………………………………………….38 2.4.16 Twitching motility……………………………………………………………………38 2.5 Biofilm formation………………………………………………………………………….39 2.5.1 Qualitative assessment of biofilm-formation in static microcosms….................39 2.5.2 Quantitative assessment of biofilm-formation in static microcosms…...............39 2.5.3 Glass bead column biofilms………………………………………………………...40 2.6 Measurement and prediction of liquid surface tension……………………………….40 2.6.1 Measurement of liquid surface tension…………………………………………….40 2.6.2 Prediction of the minimum surface tension (Min)………………………...............41 2.7 Cellulose expression……………………………………………………………………..41 2.8 Microbial attachment to hydrocarbons (MATHS assay)……………………. ……….42 2.9 Emulsion assay…………………………………………………………………………...42 2.10 Oil-displacement assay………………………………………………………………...42 2.11 Bacterial foaming assay………………………………………………………………..43 2.12 Statistics………………………………………………………………………………….43 CHAPTER 3 ISOLATION AND CHARACTERISATION OF PSEUDOMONAS SPP. BACTERIA…………………………………………………………………………………….45
Load more

Recommended publications
  • Microbial Community Structure Dynamics in Ohio River Sediments During Reductive Dechlorination of Pcbs
    University of Kentucky UKnowledge University of Kentucky Doctoral Dissertations Graduate School 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS Andres Enrique Nunez University of Kentucky Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Nunez, Andres Enrique, "MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS" (2008). University of Kentucky Doctoral Dissertations. 679. https://uknowledge.uky.edu/gradschool_diss/679 This Dissertation is brought to you for free and open access by the Graduate School at UKnowledge. It has been accepted for inclusion in University of Kentucky Doctoral Dissertations by an authorized administrator of UKnowledge. For more information, please contact [email protected]. ABSTRACT OF DISSERTATION Andres Enrique Nunez The Graduate School University of Kentucky 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS ABSTRACT OF DISSERTATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Agriculture at the University of Kentucky By Andres Enrique Nunez Director: Dr. Elisa M. D’Angelo Lexington, KY 2008 Copyright © Andres Enrique Nunez 2008 ABSTRACT OF DISSERTATION MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments. Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon.
    [Show full text]
  • Pseudomonas Pseudoalcaligenes Subsp. Citruzzi Subsp. Nov
    OO20-7713/78/OO28-0 I 17$02.OO/O INTERNATIONALJOLJRNAL OF SYSTEMATIC BACTERIOLOGY,Jan. 1978, p. 117-125 Vol. 28, No. I Copyright 0 1978 International Association of Microbiological Societies Printed in U.S. A. Pseudomonas pseudoalcaligenes subsp. citruZZi subsp. nov. N. W. SCHAAD,' G. SOWELL, JR.,' R. W. GOTH,3 R. R. COLWELL? AND R. E. WEBB3 Department of Plant Pathology, University of Georgia, Athens, Georgia .30602l; and Agricultural Research Seruice, Georgia Experiment Station, Experiment, Georgia 30212; Agricultural Research Seruice, P G G I, Beltsuille, Maryland 29705'; and Department of Microbiology, University of Maryland, College Park, Maryland 20747L Ten nonfluorescent Pseudomonas strains isolated from water-soaked lesions on cotyledons of plants of five Citrullus lanatus (watermelon) plant introductions were characterized and compared phenotypically with 22 other pseudomonads. The strains were distinguished phenotypically from other known plant pathogenic pseudomonads. The watermelon bacterium was aerobic. Cells were rod-shaped, gram negative, and motile by means of a single polar flagellum. They were nonfluorescent and grew at 41°C but not at 4°C. Oxidase production and the 2- ketogluconate reaction were positive. The 10 strains utilized p-alanine, rA-leucine, D-serine, n-propanal, ethanol, ethanolamine, citrate, and fructose for growth. No growth occurred with sucrose or glucose. Their deoxyribonucleic acid base com- position was 66 1mol% guanine plus cytosine. The bacterium is phenotypically similar to P. pseudoalcaligenes but differs from it in being pathogenic to water- melon, Cucumis melo (cantaloupe), Cucumis sativus (cucumber), and Cucurbita pep0 (squash). The name P. pseudoalcaligenes subsp. citrulli is proposed for the new subspecies, of which strain C-42 (= ATCC 29625) is the type strain.
    [Show full text]
  • Comparative Genomic Analysis of Three Pseudomonas
    microorganisms Article Comparative Genomic Analysis of Three Pseudomonas Species Isolated from the Eastern Oyster (Crassostrea virginica) Tissues, Mantle Fluid, and the Overlying Estuarine Water Column Ashish Pathak 1, Paul Stothard 2 and Ashvini Chauhan 1,* 1 Environmental Biotechnology Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL 32307, USA; [email protected] 2 Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G2P5, Canada; [email protected] * Correspondence: [email protected]; Tel.: +1-850-412-5119; Fax: +1-850-561-2248 Abstract: The eastern oysters serve as important keystone species in the United States, especially in the Gulf of Mexico estuarine waters, and at the same time, provide unparalleled economic, ecological, environmental, and cultural services. One ecosystem service that has garnered recent attention is the ability of oysters to sequester impurities and nutrients, such as nitrogen (N), from the estuarine water that feeds them, via their exceptional filtration mechanism coupled with microbially-mediated denitrification processes. It is the oyster-associated microbiomes that essentially provide these myriads of ecological functions, yet not much is known on these microbiota at the genomic scale, especially from warm temperate and tropical water habitats. Among the suite of bacterial genera that appear to interplay with the oyster host species, pseudomonads deserve further assessment because Citation: Pathak, A.; Stothard, P.; of their immense metabolic and ecological potential. To obtain a comprehensive understanding on Chauhan, A. Comparative Genomic this aspect, we previously reported on the isolation and preliminary genomic characterization of Analysis of Three Pseudomonas Species three Pseudomonas species isolated from minced oyster tissue (P.
    [Show full text]
  • Cultivation-Independent Analysis of Pseudomonas Species in Soil and in the Rhizosphere of field-Grown Verticillium Dahliae Host Plants
    Blackwell Publishing LtdOxford, UKEMIEnvironmental Microbiology1462-2912© 2006 The Authors; Journal compilation © 2006 Society for Applied Microbiology and Blackwell Publishing Ltd200681221362149Original Article Pseudomonas diversity in the rhizosphereR. Costa, J. F. Salles, G. Berg and K. Smalla Environmental Microbiology (2006) 8(12), 2136–2149 doi:10.1111/j.1462-2920.2006.01096.x Cultivation-independent analysis of Pseudomonas species in soil and in the rhizosphere of field-grown Verticillium dahliae host plants Rodrigo Costa,1 Joana Falcão Salles,2 Gabriele Berg3 rescens lineage and showed closest similarity to and Kornelia Smalla1* culturable Pseudomonas known for displaying anti- 1Federal Biological Research Centre for Agriculture and fungal properties. This report provides a better under- Forestry (BBA), Messeweg 11/12, D-38104 standing of how different factors drive Pseudomonas Braunschweig, Germany. community structure and diversity in bulk and rhizo- 2UMR 5557 Ecologie Microbienne (CNRS – Université sphere soils. Lyon 1), USC 1193 INRA, bâtiment G. Mendel, 43 boulevard du 11 Novembre 1918, F-69622 Villeurbanne, Introduction France. 3Graz University of Technology, Institute of Environmental Verticillium dahliae causes wilt of a broad range of crop Biotechnology, Petersgasse 12, A-8010 Graz, Austria. plants and significant annual yield losses worldwide (Tja- mos et al., 2000). Control of V. dahliae in soil had been largely dependent on the application of methyl bromide in Summary the field. As this ozone-depleting soil fumigant has been Despite their importance for rhizosphere functioning, recently phased-out, the use of alternative, ecologically rhizobacterial Pseudomonas spp. have been mainly friendly practices to combat V. dahliae is a subject of studied in a cultivation-based manner.
    [Show full text]
  • Rapport Nederlands
    Moleculaire detectie van bacteriën in dekaarde Dr. J.J.P. Baars & dr. G. Straatsma Plant Research International B.V., Wageningen December 2007 Rapport nummer 2007-10 © 2007 Wageningen, Plant Research International B.V. Alle rechten voorbehouden. Niets uit deze uitgave mag worden verveelvoudigd, opgeslagen in een geautomatiseerd gegevensbestand, of openbaar gemaakt, in enige vorm of op enige wijze, hetzij elektronisch, mechanisch, door fotokopieën, opnamen of enige andere manier zonder voorafgaande schriftelijke toestemming van Plant Research International B.V. Exemplaren van dit rapport kunnen bij de (eerste) auteur worden besteld. Bij toezending wordt een factuur toegevoegd; de kosten (incl. verzend- en administratiekosten) bedragen € 50 per exemplaar. Plant Research International B.V. Adres : Droevendaalsesteeg 1, Wageningen : Postbus 16, 6700 AA Wageningen Tel. : 0317 - 47 70 00 Fax : 0317 - 41 80 94 E-mail : [email protected] Internet : www.pri.wur.nl Inhoudsopgave pagina 1. Samenvatting 1 2. Inleiding 3 3. Methodiek 8 Algemene werkwijze 8 Bestudeerde monsters 8 Monsters uit praktijkteelten 8 Monsters uit proefteelten 9 Alternatieve analyse m.b.v. DGGE 10 Vaststellen van verschillen tussen de bacterie-gemeenschappen op myceliumstrengen en in de omringende dekaarde. 11 4. Resultaten 13 Monsters uit praktijkteelten 13 Monsters uit proefteelten 16 Alternatieve analyse m.b.v. DGGE 23 Vaststellen van verschillen tussen de bacterie-gemeenschappen op myceliumstrengen en in de omringende dekaarde. 25 5. Discussie 28 6. Conclusies 33 7. Suggesties voor verder onderzoek 35 8. Gebruikte literatuur. 37 Bijlage I. Bacteriesoorten geïsoleerd uit dekaarde en van mycelium uit commerciële teelten I-1 Bijlage II. Bacteriesoorten geïsoleerd uit dekaarde en van mycelium uit experimentele teelten II-1 1 1.
    [Show full text]
  • Wildlife Reserves Singapore Year Book • 2009 / 2010 Year in Review
    WILDLIFE RESERVES SINGAPORE YEAR BOOK • 2009 / 2010 YEAR IN REVIEW The reporting year was one of carefully considered expansion. While the many new exhibits, attractions and inaugural events which characterised the previous year settled into their second year of operations, WRS focused on laying the groundwork this reporting year for exciting things to come in the future. HIGHLIGHTS OF THE YEAR • Wildlife Reserves Singapore Conservation Fund (WRSCF) was launched on 10 July 2009. The Fund is an independent conservation fund dedicated primarily to native wildlife conservation and education. • WRS and WRSCF signed a memorandum of understanding to formalise collaboration with the world-renowned conservation establishment, Wildlife Conservation Society (WCS), New York, on field conservation and public education to protect biodiversity. • WRS announced that it will receive a pair of giant pandas from China Wildlife Conservation Association (CWCA) as part of the collaboration to promote giant panda conservation, raise public awareness of China wildlife conservation and to implement giant panda breeding programme. The giant pandas will be housed in River Safari. • Construction of River Safari, the fourth attraction of WRS and Asia’s first freshwater- themed wildlife attraction got underway. • Night Safari won the Best Visitor Attraction Award, adding to a total of 19 for WRS, out of 21 awards given out by STB for the best in the leisure attraction category. • A tour agency named ‘Wildlife Travel & Tours’ was set up in September 09 to provide one-stop leisure attraction offerings to tourists. • Hollywood Dinos, a travelling exhibition that employs animatronics to showcase life-size dinosaurs, was held at Jurong Bird Park from 12 December 2009 to 18 February 2010.
    [Show full text]
  • Vol. 2(1) 2008
    Vol. 2(1) 2008 Editorial (p. 3) Focus: Neighbourhood & Violence Guest Editorial Miles Hewstone, Douglas S. Massey (pp. 4–5) Hating the Neighbors: The Role of Hate Crime in the Perpetuation of Black Residiential Segregation Ami Lynch (pp. 6 – 27) Neighborhood Violence and Adolescent Friendship David Harding (pp. 28 – 55) The effects of living in segregated vs. mixed areas in Northern Ireland: A simultaneous analysis of contact and threat effects in the context of micro-level neighbourhoods Katharina Schmid, Nicole Tausch, Miles Hewstone, Joanne Hughes, Ed Cairns (pp. 56 – 71) Youth Criminality and Urban Social Conflict in the City of Rosario, Argentina Celina Del Felice (pp. 72 – 97) How Insecurity impacts on school attendance and school drop out among urban slum children in Nairobi Netsayi N. Mudege, Eliya M. Zulu, Chimaraoke Izugbara (pp. 98 – 112) How Neighborhood Disadvantage Reduces Birth Weight Emily Moiduddin, Douglas S. Massey (pp. 113 – 129) Open Section Crossing the Rubicon: Deciding to Become a Paramilitary in Northern Ireland Neil Ferguson, Mark Burgess, Ian Hollywood (pp. 130 – 137) Policing and Islamophobia in Germany – The Role of Workplace Experience Heidi Mescher (pp. 138 – 156) urn:nbn:de:0070-ijcv-2008104 ISSN: 1864-1385 IJCV : Vol. 2 (1) 2008, p. 2 2 International Journal of Conflict and Violence – IJCV The International Journal of Conflict and Violence (IJCV) is a new peer-re- viewed periodical for scientific exchange and public dissemination of the latest academic research on conflict and violence. The subjects on which the IJCV concentrates have always been the subject of interest in many different areas of academic life.
    [Show full text]
  • Università Degli Studi Di Padova Dipartimento Di Biomedicina Comparata Ed Alimentazione
    UNIVERSITÀ DEGLI STUDI DI PADOVA DIPARTIMENTO DI BIOMEDICINA COMPARATA ED ALIMENTAZIONE SCUOLA DI DOTTORATO IN SCIENZE VETERINARIE Curriculum Unico Ciclo XXVIII PhD Thesis INTO THE BLUE: Spoilage phenotypes of Pseudomonas fluorescens in food matrices Director of the School: Illustrious Professor Gianfranco Gabai Department of Comparative Biomedicine and Food Science Supervisor: Dr Barbara Cardazzo Department of Comparative Biomedicine and Food Science PhD Student: Andreani Nadia Andrea 1061930 Academic year 2015 To my family of origin and my family that is to be To my beloved uncle Piero Science needs freedom, and freedom presupposes responsibility… (Professor Gerhard Gottschalk, Göttingen, 30th September 2015, ProkaGENOMICS Conference) Table of Contents Table of Contents Table of Contents ..................................................................................................................... VII List of Tables............................................................................................................................. XI List of Illustrations ................................................................................................................ XIII ABSTRACT .............................................................................................................................. XV ESPOSIZIONE RIASSUNTIVA ............................................................................................ XVII ACKNOWLEDGEMENTS ....................................................................................................
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 7476,532 B2 Schneider Et Al
    USOO7476532B2 (12) United States Patent (10) Patent No.: US 7476,532 B2 Schneider et al. (45) Date of Patent: Jan. 13, 2009 (54) MANNITOL INDUCED PROMOTER Makrides, S.C., "Strategies for achieving high-level expression of SYSTEMIS IN BACTERAL, HOST CELLS genes in Escherichia coli,” Microbiol. Rev. 60(3):512-538 (Sep. 1996). (75) Inventors: J. Carrie Schneider, San Diego, CA Sánchez-Romero, J., and De Lorenzo, V., "Genetic engineering of nonpathogenic Pseudomonas strains as biocatalysts for industrial (US); Bettina Rosner, San Diego, CA and environmental process.” in Manual of Industrial Microbiology (US) and Biotechnology, Demain, A, and Davies, J., eds. (ASM Press, Washington, D.C., 1999), pp. 460-474. (73) Assignee: Dow Global Technologies Inc., Schneider J.C., et al., “Auxotrophic markers pyrF and proC can Midland, MI (US) replace antibiotic markers on protein production plasmids in high cell-density Pseudomonas fluorescens fermentation.” Biotechnol. (*) Notice: Subject to any disclaimer, the term of this Prog., 21(2):343-8 (Mar.-Apr. 2005). patent is extended or adjusted under 35 Schweizer, H.P.. "Vectors to express foreign genes and techniques to U.S.C. 154(b) by 0 days. monitor gene expression in Pseudomonads. Curr: Opin. Biotechnol., 12(5):439-445 (Oct. 2001). (21) Appl. No.: 11/447,553 Slater, R., and Williams, R. “The expression of foreign DNA in bacteria.” in Molecular Biology and Biotechnology, Walker, J., and (22) Filed: Jun. 6, 2006 Rapley, R., eds. (The Royal Society of Chemistry, Cambridge, UK, 2000), pp. 125-154. (65) Prior Publication Data Stevens, R.C., “Design of high-throughput methods of protein pro duction for structural biology.” Structure, 8(9):R177-R185 (Sep.
    [Show full text]
  • Xylella Fastidiosa Biologia I Epidemiologia
    Xylella fastidiosa Biologia i epidemiologia Emili Montesinos Seguí Catedràtic de Producció Vegetal (Patologia Vegetal) Universitat de Girona [email protected] www.youtube.com/watch?v=sur5VzJslcM Xylella fastidiosa, un patogen que no és nou Newton B. Pierce (1890s, USA) Agrobacterium tumefaciens Chlamydiae Proteobacteria Bartonella bacilliformis Campylobacter coli Bartonella henselae CDC Chlamydophila psittaci Campylobacter fetus Bartonella quintana Bacteroides fragilis CDC Brucella melitensis Bacteroidetes Chlamydophila pneumoniae Campylobacter hyointestinalis Bacteroides thetaiotaomicron Campylobacter jejuni CDC Brucella melitensis biovar Abortus CDC Chlamydia trachomatis Capnocytophaga canimorus Campylobacter lari CDC Brucella melitensis biovar Canis Chryseobacterium meningosepticum Parachlamydia acanthamoebae Campylobacter upsaliensis CDC Brucella melitensis biovar Suis Helicobacter pylori Candidatus Liberibacter africanus CDC Candidatus Liberibacter asiaticus Borrelia burgdorferi Epsilon Borrelia hermsii CDC Anaplasma phagocytophilum Borrelia recurrentis Alpha CDC Ehrlichia canis Spirochetes Borrelia turicatae CDC Ehrlichia chaffeensis Eikenella corrodens Leptospira interrogans CDC Ehrlichia ewingii CDC CDC Neisseria gonorrhoeae Treponema pallidum Ehrlichia ruminantium CDC Neisseria meningitidis CDC Neorickettsia sennetsu Spirillum minus Orientia tsutsugamushi Fusobacterium necrophorum Beta Fusobacteria CDC Bordetella pertussis Rickettsia conorii Streptobacillus moniliformis Burkholderia cepacia Rickettsia
    [Show full text]
  • Pseudomonas Gessardii Sp. Nov. and Pseudornonas Migulae Sp. Nov., Two New Species Isolated from Natural Mineral Waters
    International Journal of Systematic Bacteriology (1 999), 49, 1 559-1 572 Printed in Great Britain Pseudomonas gessardii sp. nov. and Pseudornonas migulae sp. nov., two new species isolated from natural mineral waters Sophie Verhille,l Nader Batda,' Fouad Dabboussi,' Monzer Hamze,* Daniel Izard' and Henri Leclerc' Author for correspondence: Henri Leclerc. Tel: + 33 3 20 52 94 28. Fax: + 33 3 20 52 93 61. e-mail : leclerc(@univ-lille2.fr Service de Bact6riologie- Twenty-f ive non-identif ied fluorescent Pseudomonas strains isolated from Hygihne, Facult6 de natural mineral waters were previously clustered into three phenotypic Medecine Henri Warembourg (p81e subclusters, Xlllb, XVa and XVc. These strains were characterized genotypically recherche), 1 place de in the present study. DNA-DNA hybridization results and DNA base Verdun, 59045 Lille Cedex, composition analysis revealed that these strains were members of two new France species, for which the names Pseudomonas gessardii sp. nov. (type strain CIP * Facult6 de Sant6 Publique, 1054693 and Pseudomonas migulae sp. nov. (type strain CIP 1054703 are U n iversite Liba na ise, Tripoli, Lebanon and CNRS proposed. P. gessardii included 13 strains from phenotypic subclusters XVa and Liban, Beirut, Lebanon XVc. P. migulae included 10 strains from phenotypic subcluster Xlllb. The levels of DNA-DNA relatedness ranged from 71 to 100% for P. gessardii and from 74 to 100% for P. migulae. The G+C content of the DNA of each type strain was 58 mol%. DNA similarity levels, measured with 67 reference strains of Pseudomonas species, were below 55%, with ATm values of 13 "C or more.
    [Show full text]
  • Characterisation of Pseudomonas Spp. Isolated from Foods
    07.QXD 9-03-2007 15:08 Pagina 39 Annals of Microbiology, 57 (1) 39-47 (2007) Characterisation of Pseudomonas spp. isolated from foods Laura FRANZETTI*, Mauro SCARPELLINI Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Agraria Alimentare Ecologica, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy Received 30 June 2006 / Accepted 27 December 2006 Abstract - Putative Pseudomonas spp. (102 isolates) from different foods were first characterised by API 20NE and then tested for some enzymatic activities (lipase and lecithinase production, starch hydrolysis and proteolytic activity). However subsequent molecular tests did not always confirm the results obtained, thus highlighting the limits of API 20NE. Instead RFLP ITS1 and the sequencing of 16S rRNA gene grouped the isolates into 6 clusters: Pseudomonas fluorescens (cluster I), Pseudomonas fragi (cluster II and V) Pseudomonas migulae (cluster III), Pseudomonas aeruginosa (cluster IV) and Pseudomonas chicorii (cluster VI). The pectinolytic activity was typical of species isolated from vegetable products, especially Pseudomonas fluorescens. Instead Pseudomonas fragi, predominantly isolated from meat was characterised by proteolytic and lipolytic activities. Key words: Pseudomonas fluorescens, enzymatic activity, ITS1. INTRODUCTION the most frequently found species, however the species dis- tribution within the food ecosystem remains relatively The genus Pseudomonas is the most heterogeneous and unknown (Arnaut-Rollier et al., 1999). The principal micro- ecologically significant group of known bacteria, and bial population of many vegetables in the field consists of includes Gram-negative motile aerobic rods that are wide- species of the genus Pseudomonas, especially the fluores- spread throughout nature and characterised by elevated cent forms.
    [Show full text]