DOCSLIB.ORG

Xylella Fastidiosa Biologia I Epidemiologia

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Xylella fastidiosa Biologia i epidemiologia Emili Montesinos Seguí Catedràtic de Producció Vegetal (Patologia Vegetal) Universitat de Girona [email protected] www.youtube.com/watch?v=sur5VzJslcM Xylella fastidiosa, un patogen que no és nou Newton B. Pierce (1890s, USA) Agrobacterium tumefaciens Chlamydiae Proteobacteria Bartonella bacilliformis Campylobacter coli Bartonella henselae CDC Chlamydophila psittaci Campylobacter fetus Bartonella quintana Bacteroides fragilis CDC Brucella melitensis Bacteroidetes Chlamydophila pneumoniae Campylobacter hyointestinalis Bacteroides thetaiotaomicron Campylobacter jejuni CDC Brucella melitensis biovar Abortus CDC Chlamydia trachomatis Capnocytophaga canimorus Campylobacter lari CDC Brucella melitensis biovar Canis Chryseobacterium meningosepticum Parachlamydia acanthamoebae Campylobacter upsaliensis CDC Brucella melitensis biovar Suis Helicobacter pylori Candidatus Liberibacter africanus CDC Candidatus Liberibacter asiaticus Borrelia burgdorferi Epsilon Borrelia hermsii CDC Anaplasma phagocytophilum Borrelia recurrentis Alpha CDC Ehrlichia canis Spirochetes Borrelia turicatae CDC Ehrlichia chaffeensis Eikenella corrodens Leptospira interrogans CDC Ehrlichia ewingii CDC CDC Neisseria gonorrhoeae Treponema pallidum Ehrlichia ruminantium CDC Neisseria meningitidis CDC Neorickettsia sennetsu Spirillum minus Orientia tsutsugamushi Fusobacterium necrophorum Beta Fusobacteria CDC Bordetella pertussis Rickettsia conorii Streptobacillus moniliformis Burkholderia cepacia Rickettsia felis Burkholderia mallei Rickettsia prowazekii CDC Firmicutes CDC Rickettsia rickettsii Staphylococcus aureus Burkholderia pseudomallei Gamma Enterotoxin A Ralstonia mannitolilytica Rickettsia typhi Enterococcus faecalis Ralstonia pickettii Enterotoxin B CDC Francisella tularensis Enterococcus faecium Ralstonia solanacearum Staphylococcus, coagulase-negative Tatlockia micdadei Enterococcus flavescens Staphylococcus epidermidis Legionella longbeachae Stentrophomonas maltophila Streptococcus acidominimus Staphylococcus haemolyticus CDC Legionella pneumophila Xanthomonas campestris pv. malvacearum Streptococcus agalactiae Staphylococcus intermedius CDC Xanthomonas oryzae pv. oryzae Streptococcus bovis Staphylococcus saprophyticus Coxiella burnetti Streptococcus canis Xanthomonas oryzae pv. oryzicola Listeria ivanovii Streptococcus criceti Acinetobacter baumannii Pseudomonas syringae pv. glycinea Xylella fastidiosa CDC Listeria monocytogenes Streptococcus equi Acinetobacter lwoffii Pseudomonas aeruginosa Alteromonas tetraodonis Listeria seeligeri Streptococcus intermedius Acinetobacter radioresistans Pseudomonas alcaligenes Tetrodotoxin Listeria welshimeri Streptococcus milleri Moraxella catarrhalis Pseudomonas fluorescens CDC CDC CDC Acinetobacter calcoaceticus Haemophilus influenzae Streptococcus pneumoniae Bacillus anthracis Pseudomonas putida Aeromonas sp. CDC Streptococcus pyogenes Acinetobacter calcoaceticus Pseudomonas stutzeri Haemophilus aegyptius Bacillus cereus Streptococcus suis subsp. Anitratus CDC Haemophilus ducreyi Streptococcus uberis Bacilli Bacillus subtilis Haemophilus parahaemolyticus Streptococcus viridans CDC Vibrio cholerae Haemophilus parainfluenzae Cholera Toxin Haemophilus paraphrophilus Mycoplasma pneumoniae Vibrio mimicus Pasteurella multocida Erysipelothrix rhusiopathiae Vibrio parahaemolyticus Mycoplasma mycoides Actinobacteria Mollicutes Vibrio vulnificus Mycoplasma capricolum CDC Mycobacterium tuberculosis CDC Escherichia coli subsp. Capripneumoniae Mycobacterium abscessus Shiga Toxin Rhodococcus equi Mycobacterium avium Salmonella bongori Clostridia Actinomyces israelii Citrobacter freundii Salmonella choleraesuis Anaerococcus prevotii Mycobacterium bovis Actinomyces naeslundii Edwardsiella tarda CDC Salmonella enteriditis CDC Clostridium botulinum Mycobacterium fortuitum Propionibacterium acnes Enterobacter aerogenes Salmonella paratyphi Botulinum toxin Mycobacterium kansasii Rothia dentocariosa Enterobacter cloacae CDC Salmonella typhimurium Clostridium difficile CDC Mycobacterium leprae Clavibacter michiganensis Klebsiella oxytoca Klebsiella pneumoniae CDC Salmonella typhi Clostridium perfringens Mycobacterium haemophilum subsp. Sepedonicus CDC Nocardia asteroides Morganella morganii Shigella boydii Epsilon toxin Mycobacterium marinum CDC Nocardia nova Proteus mirabilis Shigella dysenteriae Clostridium septicum Mycobacterium scrofulaceum CDC Shigella flexneri CDC Corynebacterium amycolatum Proteus vulgaris Clostridium tetani Mycobacterium simiae CDC Providencia stuartii Shigella sonnei Peptococcus niger Corynebacterium jeikeium Mycobacterium szulgai Serratia marcescens Yersinia enterocolitica Peptostreptococcus anaerobius Corynebacterium xerosis Mycobacterium ulcerans Yersinia pseudotuberculosis CDC Corynebacterium diphtheriae Veillonella dispar Mycobacterium xenopi CDC Yersinia pestis Diphtheria toxin Ecker et al. BMC Microbiology 2005 5:19 doi:10.1186/1471-2180-5-19 MALALTIES CAUSADES PER Xylella fastidiosa Malaltia de Pierce (vid) Clorosi variegada dels citrics http://ucce.ucdavis.edu/files/repository/ calag/img6801p20.jpg http://www.ivia.es/iocv/enfermedades/ Mort sobtada de les oliveres Asurat del baladre (Nereum oleander) http://www.eppo.int/QUARANTINE/special_topics/ http://orange.ifas.ufl.edu/cfnurseries/pdffiles/ Xylella_fastidiosa/Xylella_fastidiosa.htm plantfacts/Oleander.pdf ULTRAESTRUCTURA TEM http://www.apsnet.org/edcenter/intropp/lessons/prokaryotes/Pages/ BacterialLeafScorch.aspx Colonització dels vasos del xilema http://www.padil.gov.au/pests-and-diseases/pest/main/136652/3760 CULTIU Medi PCYE 1.0g KH2PO4 1.1g K HPO 2 4 0.25g FeSO4.7H2O 0.4g Mg SO4.7H2O 2.0 g carbó activat 10.0 g extracte de llevat, 1 L aigua destil.lada 8.0 g Gelrite o 10.0 g agar-agar. pH=6.9 con 1 M KOH Autoclavar Colònies en Agar (1-3 mm, 15-30 dies) Lopes y Torres. Current Microbiol. Vol. 53 (2006), pp. 467–469 http://www.apsnet.org/edcenter/intropp/ lessons/prokaryotes/Pages/ BacterialLeafScorch.aspx DIVERSITAT subsp. fastidiosa (xff): causa la malaltia de Pierce en vid en la UE i asurat de la fulla de l’ametller (almond leaf scorch). subsp. multiplex (xfm): produeix asurat (leaf scorch diseases) en una gran nombre de arbres i és endèmica de Nord Amèrica. Afecta a fruiters de pinyol, Quercus i pecans. subsp. pauca (xfp): causa la clorosi variegada dels cítrics (Brasil), Asurat de la fulla del cafeto a Sud Amèrica, del baladre a Costa Rica. subsp. sandyi (xfs): responsable del asurat de fulles del baladre a California y Texas (USA). També afecta a magnolies i xicrandes. Plantes hoste de Xylella fastidiosa Subespècie Hoste Gènere Espècie fastidiosa 42 138 164 multiplex 28 69 84 pauca 16 30 36 sandyi 5 6 5 Font: EFSA Journal 2015;13(1):3989. Xylella fastidiosa pest risk assessment ALTRES PLANTES HOSTE DE X. fastidiosa http://www.cnr.berkeley.edu/xylella/index.html I MOLTS MÉS … GENOMA CARÁCTERÍSTIQUES DEL GENOMA DE X. fastidiosa Soques seqüenciades: 9a5c (taronger, 1992) Ann-1 (baladre) Temecula-1 (vid, 1998) Dixon (ametller) 2.390-2.730 Kb 1600 gens (varis plàsmids) • absència de gens d’avirulència (avr) • toxines (hemolisines, bacteriocines, poliquètids…) • exomucopolisacàrids (xantans!). Agregació, taponament. • fímbries (adhesió, biofilm) • sistema secretor Tipus II (enzims) • betaglucanases (PG, CEL). Migració xilema • proteïnes de transport (ions, aa, ch, etc.). Nutrició • metabolisme oxidatiu (glucòlisi, Krebs, ETS) AÏLLAT D’APULIA (ITÀLIA) EN OLIVERES crossmark Draft Genome Sequence of the Xylella fastidiosa CoDiRO Strain Annalisa Giampetruzzi,a Michela Chiumenti,a Maria Saponari,a Giacinto Donvito,b Alessandro Italiano,b Giuliana Loconsole,a Donato Boscia,a Corrado Cariddi,c Giovanni Paolo Martelli,c Pasquale Saldarellia Institute for Sustainable Plant Protection, National Research Council (CNR), Bari, Italya; Department of Physics, University of Bari Aldo Moro, Bari, Italyb; Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari, Italyc A.G. and M.C. contributed equally to this work. We determined the draft genome sequence of the Xylella fastidiosa CoDiRO strain, which has been isolated from olive plants in southern Italy (Apulia). It is associated with olive quick decline syndrome (OQDS) and characterized by extensive scorching and desiccation of leaves and twigs. Received 22 December 2014 Accepted 5 January 2015 Published 12 February 2015 Citation Giampetruzzi A, Chiumenti M, Saponari M, Donvito G, Italiano A, Loconsole G, Boscia D, Cariddi C, Martelli GP, Saldarelli P. 2015. Draft genome sequence of the Xylella fastidiosa CoDiRO strain. Genome Announc 3(1):e01538-14. doi: 10.1128/genomeA.01538-14. Copyright © 2015 Giampetruzzi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. Address correspondence to Annalisa Giampetruzzi, [email protected]. ylella fastidiosa is a xylem-restricted Gram-negative bacterium resulting in the identification of 6 rRNA genes, 49 tRNA loci, 2,053 Xand the agent of diseases of a wide range of hosts (1). Currently, protein-encoding genes, and 2 noncoding RNAs. A plasmid of Genomaseven X. fastidiosa genomes: 2.508 have been completelyKb (+ sequenced, pXf35.3), in- 35,318 bp was 2053 also found sharing proteines 98% similarity in the tra and trb cluding citrus-variegated chlorosis strain 9a5c, Pierce’s disease loci with the conjugative plasmid pXF-RIV5 (10), but differing strains Temecula
Recommended publications
  • Evidence for Niche Adaptation in the Genome of The

    Evidence for Niche Adaptation in the Genome of The

    University of Warwick institutional repository: http://go.warwick.ac.uk/wrap This paper is made available online in accordance with publisher policies. Please scroll down to view the document itself. Please refer to the repository record for this item and our policy information available from the repository home page for further information. To see the final version of this paper please visit the publisher’s website. Access to the published version may require a subscription. Author(s): Philip N Ward, Matthew TG Holden, James A Leigh, Nicola Lennard, Alexandra Bignell, Andy Barron, Louise Clark, Michael A Quail, John Woodward, Bart G Barrell, Sharon A Egan, Terence R Field, Duncan Maskell, Michael Kehoe, Christopher G Dowson, Neil Chanter, Adrian M Whatmore, Stephen D Bentley and Julian Parkhill Article Title: Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis Year of publication: 2009 Link to published version: http://dx.doi.org/ doi: doi:10.1186/1471-2164- 10-54 Publisher statement: None BMC Genomics BioMed Central Research article Open Access Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis Philip N Ward1, Matthew TG Holden*2, James A Leigh3, Nicola Lennard2, Alexandra Bignell2, Andy Barron2, Louise Clark2, Michael A Quail2, John Woodward2, Bart G Barrell2, Sharon A Egan3, Terence R Field4, Duncan Maskell5, Michael Kehoe6, Christopher G Dowson7, Neil Chanter8, Adrian M Whatmore7,9, Stephen D Bentley2 and Julian Parkhill2 Address: 1Nuffield Department of Clinical Laboratory Sciences, Oxford University, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK, 2The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK, 3The School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire, LE12 5RD, UK, 4Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks, RG20 7NN, UK, 5Dept.
  • Arginine Metabolism in the Edwardsiella Ictaluri

    Arginine Metabolism in the Edwardsiella Ictaluri

    Louisiana State University LSU Digital Commons LSU Doctoral Dissertations Graduate School 2011 Arginine metabolism in the Edwardsiella ictaluri- channel catfish macrophage dynamic Wes Arend Baumgartner Louisiana State University and Agricultural and Mechanical College, [email protected] Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_dissertations Part of the Veterinary Pathology and Pathobiology Commons Recommended Citation Baumgartner, Wes Arend, "Arginine metabolism in the Edwardsiella ictaluri- channel catfish macrophage dynamic" (2011). LSU Doctoral Dissertations. 2821. https://digitalcommons.lsu.edu/gradschool_dissertations/2821 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Doctoral Dissertations by an authorized graduate school editor of LSU Digital Commons. For more information, please [email protected]. ARGININE METABOLISM IN THE EDWARDSIELLA ICTALURI- CHANNEL CATFISH MACROPHAGE DYNAMIC A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Interdepartmental Program in Veterinary Medical Sciences Through the Department of Pathobiological Sciences by Wes Arend Baumgartner B.S., University of Illinois, 1998 D.V.M., University of Illinois, 2002 Dipl. ACVP, 2009 December 2011 DEDICATION This work is dedicated to: my wife Denise who makes
  • Microbial Community Structure Dynamics in Ohio River Sediments During Reductive Dechlorination of Pcbs

    Microbial Community Structure Dynamics in Ohio River Sediments During Reductive Dechlorination of Pcbs

    University of Kentucky UKnowledge University of Kentucky Doctoral Dissertations Graduate School 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS Andres Enrique Nunez University of Kentucky Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Nunez, Andres Enrique, "MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS" (2008). University of Kentucky Doctoral Dissertations. 679. https://uknowledge.uky.edu/gradschool_diss/679 This Dissertation is brought to you for free and open access by the Graduate School at UKnowledge. It has been accepted for inclusion in University of Kentucky Doctoral Dissertations by an authorized administrator of UKnowledge. For more information, please contact [email protected]. ABSTRACT OF DISSERTATION Andres Enrique Nunez The Graduate School University of Kentucky 2008 MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS ABSTRACT OF DISSERTATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Agriculture at the University of Kentucky By Andres Enrique Nunez Director: Dr. Elisa M. D’Angelo Lexington, KY 2008 Copyright © Andres Enrique Nunez 2008 ABSTRACT OF DISSERTATION MICROBIAL COMMUNITY STRUCTURE DYNAMICS IN OHIO RIVER SEDIMENTS DURING REDUCTIVE DECHLORINATION OF PCBS The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments. Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon.
  • Pseudomonas Pseudoalcaligenes Subsp. Citruzzi Subsp. Nov

    Pseudomonas Pseudoalcaligenes Subsp. Citruzzi Subsp. Nov

    OO20-7713/78/OO28-0 I 17$02.OO/O INTERNATIONALJOLJRNAL OF SYSTEMATIC BACTERIOLOGY,Jan. 1978, p. 117-125 Vol. 28, No. I Copyright 0 1978 International Association of Microbiological Societies Printed in U.S. A. Pseudomonas pseudoalcaligenes subsp. citruZZi subsp. nov. N. W. SCHAAD,' G. SOWELL, JR.,' R. W. GOTH,3 R. R. COLWELL? AND R. E. WEBB3 Department of Plant Pathology, University of Georgia, Athens, Georgia .30602l; and Agricultural Research Seruice, Georgia Experiment Station, Experiment, Georgia 30212; Agricultural Research Seruice, P G G I, Beltsuille, Maryland 29705'; and Department of Microbiology, University of Maryland, College Park, Maryland 20747L Ten nonfluorescent Pseudomonas strains isolated from water-soaked lesions on cotyledons of plants of five Citrullus lanatus (watermelon) plant introductions were characterized and compared phenotypically with 22 other pseudomonads. The strains were distinguished phenotypically from other known plant pathogenic pseudomonads. The watermelon bacterium was aerobic. Cells were rod-shaped, gram negative, and motile by means of a single polar flagellum. They were nonfluorescent and grew at 41°C but not at 4°C. Oxidase production and the 2- ketogluconate reaction were positive. The 10 strains utilized p-alanine, rA-leucine, D-serine, n-propanal, ethanol, ethanolamine, citrate, and fructose for growth. No growth occurred with sucrose or glucose. Their deoxyribonucleic acid base com- position was 66 1mol% guanine plus cytosine. The bacterium is phenotypically similar to P. pseudoalcaligenes but differs from it in being pathogenic to water- melon, Cucumis melo (cantaloupe), Cucumis sativus (cucumber), and Cucurbita pep0 (squash). The name P. pseudoalcaligenes subsp. citrulli is proposed for the new subspecies, of which strain C-42 (= ATCC 29625) is the type strain.
  • Comparative Genomic Analysis of Three Pseudomonas

    Comparative Genomic Analysis of Three Pseudomonas

    microorganisms Article Comparative Genomic Analysis of Three Pseudomonas Species Isolated from the Eastern Oyster (Crassostrea virginica) Tissues, Mantle Fluid, and the Overlying Estuarine Water Column Ashish Pathak 1, Paul Stothard 2 and Ashvini Chauhan 1,* 1 Environmental Biotechnology Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL 32307, USA; [email protected] 2 Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G2P5, Canada; [email protected] * Correspondence: [email protected]; Tel.: +1-850-412-5119; Fax: +1-850-561-2248 Abstract: The eastern oysters serve as important keystone species in the United States, especially in the Gulf of Mexico estuarine waters, and at the same time, provide unparalleled economic, ecological, environmental, and cultural services. One ecosystem service that has garnered recent attention is the ability of oysters to sequester impurities and nutrients, such as nitrogen (N), from the estuarine water that feeds them, via their exceptional filtration mechanism coupled with microbially-mediated denitrification processes. It is the oyster-associated microbiomes that essentially provide these myriads of ecological functions, yet not much is known on these microbiota at the genomic scale, especially from warm temperate and tropical water habitats. Among the suite of bacterial genera that appear to interplay with the oyster host species, pseudomonads deserve further assessment because Citation: Pathak, A.; Stothard, P.; of their immense metabolic and ecological potential. To obtain a comprehensive understanding on Chauhan, A. Comparative Genomic this aspect, we previously reported on the isolation and preliminary genomic characterization of Analysis of Three Pseudomonas Species three Pseudomonas species isolated from minced oyster tissue (P.
  • Melanaphis Sacchari), in Grain Sorghum

    Melanaphis Sacchari), in Grain Sorghum

    DEVELOPMENT OF A RESEARCH-BASED, USER- FRIENDLY, RAPID SCOUTING PROCEDURE FOR THE INVASIVE SUGARCANE APHID (MELANAPHIS SACCHARI), IN GRAIN SORGHUM By JESSICA CARRIE LINDENMAYER Bachelor of Science in Soil and Crop Sciences Bachelor of Science in Horticulture Colorado State University Fort Collins, Colorado 2013 Master of Science in Entomology and Plant Pathology Oklahoma State University Stillwater, Oklahoma 2015 Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of DOCTOR OF PHILOSOPHY May, 2019 DEVELOPMENT OF A RESEARCH-BASED, USER- FRIENDLY, RAPID SCOUTING PROCEDURE FOR THE INVASIVE SUGARCANE APHID (MELANAPHIS SACCHARI), IN GRAIN SORGHUM Dissertation Approved: Tom A. Royer Dissertation Adviser Kristopher L. Giles Norman C. Elliott Mark E. Payton ii ACKNOWLEDGEMENTS I would like to thank my amazing committee and all my friends and family for their endless support during my graduate career. I would like to say a special thank you to my husband Brad for supporting me in every way one possibly can, I couldn’t have pursued this dream without you. I’d also like to thank my first child, due in a month. The thought of getting to be your mama pushed me to finish strong so you would be proud of me. Lastly, I want to thank my step father Jasper H. Davis III for showing me how to have a warrior’s spirit and to never give up on something, or someone you love. Your love, spirit, and motivational words will always be heard in my heart even while you’re gone.
  • Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis

    Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis

    International Journal of Molecular Sciences Review Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis David Johane Machate 1, Priscila Silva Figueiredo 2 , Gabriela Marcelino 2 , Rita de Cássia Avellaneda Guimarães 2,*, Priscila Aiko Hiane 2 , Danielle Bogo 2, Verônica Assalin Zorgetto Pinheiro 2, Lincoln Carlos Silva de Oliveira 3 and Arnildo Pott 1 1 Graduate Program in Biotechnology and Biodiversity in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] (D.J.M.); [email protected] (A.P.) 2 Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; pri.fi[email protected] (P.S.F.); [email protected] (G.M.); [email protected] (P.A.H.); [email protected] (D.B.); [email protected] (V.A.Z.P.) 3 Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] * Correspondence: [email protected]; Tel.: +55-67-3345-7416 Received: 9 March 2020; Accepted: 27 March 2020; Published: 8 June 2020 Abstract: Long-term high-fat dietary intake plays a crucial role in the composition of gut microbiota in animal models and human subjects, which affect directly short-chain fatty acid (SCFA) production and host health. This review aims to highlight the interplay of fatty acid (FA) intake and gut microbiota composition and its interaction with hosts in health promotion and obesity prevention and its related metabolic dysbiosis.
  • Bacillus Pseudomycoides Sp. Nov

    Bacillus Pseudomycoides Sp. Nov

    international Journal of Systematic Bacteriology (1 998), 48, 103 1-1 035 Printed in Great Britain Bacillus pseudomycoides sp. nov. L. K. Nakamura Tel: + 1 309 681 6395. Fax: + 1 309 681 6672. e-mail: [email protected] Microbial Properties Previous DNA relatedness studies showed that strains identified as Bacillus Research, National Center mycoides segregated into two genetically distinct yet phenotypically similar for Agricultural Utilization Research, Agricu Itura I groups, one being B. mycoides sensu stricto and the other, an unclassified Research Service, US taxon. In the present study, the taxonomic position of this second group was Department of assessed by measuring DNA relatedness and determining phenotypic Agricu Iture, Peoria, IL 61604, USA characteristics of an increased number of B. mycoides strains. Also determined was the second group's 165 RNA gene sequence. The 36 B. mycoides strains studied segregated into two genetically distinct groups showing DNA relatedness of about 30%; 18 strains represented the species proper and 18 the second group with intragroup DNA relatedness for both groups ranging from 70 to 100%. DNA relatedness to the type strains of presently recognized species with G+C contents of approximately 35 molO/i (Bacillus alcalophilus, Bacillus cereus, Bacillus circulans, Bacillus lentus, Bacillus megaterium and Bacillus sphaericus) ranged from 22 to 37%. Although shown to be genetically distinct taxa, the two B. mycoides groups exhibited highly similar (98%) 165 RNA sequences. Phylogenetic analyses showed that both B. mycoides and the second group clustered closely with B. cereus. Although not distinguishable by physiological and morphological characteristics, the two B. mycoides groups and B.
  • Food Microbiology 55 (2016) 73E85

    Food Microbiology 55 (2016) 73E85

    Food Microbiology 55 (2016) 73e85 Contents lists available at ScienceDirect Food Microbiology journal homepage: www.elsevier.com/locate/fm Transcriptome analysis of Bacillus thuringiensis spore life, germination and cell outgrowth in a vegetable-based food model Daniela Bassi a, Francesca Colla a, 1, Simona Gazzola a, Edoardo Puglisi a, * Massimo Delledonne b, Pier Sandro Cocconcelli a, a Istituto di Microbiologia e Centro Ricerche Biotecnologiche, Universita Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy e via Milano 24, 26100 Cremona, Italy b Dipartimento di Biotecnologie, Universita degli Studi di Verona, Strada le Grazie 15, 37134 Verona, Italy article info abstract Article history: Toxigenic species belonging to Bacillus cereus sensu lato, including Bacillus thuringiensis, cause foodborne Received 23 April 2015 outbreaks thanks to their capacity to survive as spores and to grow in food matrixes. The goal of this Received in revised form work was to assess by means of a genome-wide transcriptional assay, in the food isolate B. thuringiensis 3 November 2015 UC10070, the gene expression behind the process of spore germination and consequent outgrowth in a Accepted 10 November 2015 vegetable-based food model. Scanning electron microscopy and Energy Dispersive X-ray microanalysis Available online 12 November 2015 were applied to select the key steps of B. thuringiensis UC10070 cell cycle to be analyzed with DNA- microarrays. At only 40 min from heat activation, germination started rapidly and in less than two Keywords: Bacillus cereus sensu lato hours spores transformed in active growing cells. A total of 1646 genes were found to be differentially Food model expressed and modulated during the entire B.
  • FUNGI ASSOCIATED with the GLASSY-WINGED SHARPSHOOTER, Homalodisca Coagulata, in ITS NATIVE RANGE

    FUNGI ASSOCIATED with the GLASSY-WINGED SHARPSHOOTER, Homalodisca Coagulata, in ITS NATIVE RANGE

    FUNGI ASSOCIATED WITH THE GLASSY-WINGED SHARPSHOOTER, Homalodisca coagulata, IN ITS NATIVE RANGE By S. ELIE BREAUX A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2005 Copyright 2005 by S. Elie Breaux This document is dedicated to Stefanie, always there. ACKNOWLEDGMENTS I would like to thank the members of my committee for their support, perseverance, and knowledge. I consider myself lucky to have found in them the willingness to take a chance on a student. I would like to thank Dr. Linda Young for extensive assistance in the statistical analysis portion of this study. I would also like to thank my family. My father has always been a student of nature. Raised with his love of the outdoors, the choice to take this path was made without reservation. My mother has always provided every kind of support a son could ask for, free of expectation or judgment. I thank Nicholas and Silas for being so entertaining. They are so different in nature, but time spent with either of them makes one realize what is important. And finally, I would like to thank Stefanie. Always generous with encouragement and unwavering in support, there is no way I could have done this without her. iv TABLE OF CONTENTS page ACKNOWLEDGMENTS ................................................................................................. iv LIST OF TABLES...........................................................................................................
  • A Survey of Carbon Fixation Pathways Through a Quantitative Lens

    A Survey of Carbon Fixation Pathways Through a Quantitative Lens

    Journal of Experimental Botany, Vol. 63, No. 6, pp. 2325–2342, 2012 doi:10.1093/jxb/err417 Advance Access publication 26 December, 2011 REVIEW PAPER A survey of carbon fixation pathways through a quantitative lens Arren Bar-Even, Elad Noor and Ron Milo* Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel * To whom correspondence should be addressed. E-mail: [email protected] Received 15 August 2011; Revised 4 November 2011; Accepted 8 November 2011 Downloaded from Abstract While the reductive pentose phosphate cycle is responsible for the fixation of most of the carbon in the biosphere, it http://jxb.oxfordjournals.org/ has several natural substitutes. In fact, due to the characterization of three new carbon fixation pathways in the last decade, the diversity of known metabolic solutions for autotrophic growth has doubled. In this review, the different pathways are analysed and compared according to various criteria, trying to connect each of the different metabolic alternatives to suitable environments or metabolic goals. The different roles of carbon fixation are discussed; in addition to sustaining autotrophic growth it can also be used for energy conservation and as an electron sink for the recycling of reduced electron carriers. Our main focus in this review is on thermodynamic and kinetic aspects, including thermodynamically challenging reactions, the ATP requirement of each pathway, energetic constraints on carbon fixation, and factors that are expected to limit the rate of the pathways. Finally, possible metabolic structures at Weizmann Institute of Science on July 3, 2016 of yet unknown carbon fixation pathways are suggested and discussed.
  • Bacillus Pseudomycoides Sp. Nov. NOTE L

    Bacillus Pseudomycoides Sp. Nov. NOTE L

    International Journal ofSystematic Bacteriology (1998),48,1031-1035 Printed in Great Britain Bacillus pseudomycoides sp. nov. NOTE L. K. Nakamura Tel: + I 309681 6395. Fax: + I 3096816672. e-mail: [email protected] Microbial Properties Previous DNA relatedness studies showed that strains identified as Bacillus Research, National Center mycoides segregated into two genetically distinct yet phenotypically similar for Agricultural Utilization Research, Agricultural groups, one being B. mycoides sensu stricto and the other, an unclassified Research Service, US taxon. In the present study, the taxonomic position of this second group was Department of assessed by measuring DNA relatedness and determining phenotypic Agriculture, Peoria, IL 61604, USA characteristics of an increased number of B. mycoides strains. Also determined was the second group's 165 RNA gene sequence. The 36 B. mycoides strains studied segregated into two genetically distinct groups showing DNA relatedness of about 30%; 18 strains represented the species proper and 18 the second group with intragroup DNA relatedness for both groups ranging from 70 to 100%. DNA relatedness to the type strains of presently recognized species with G+C contents of approximately 35 mol% (Bacillus alcalophilus, Bacillus cereus, Bacillus circulans, Bacillus lentus, Bacillus megaterium and Bacillus sphaericus) ranged from 22 to 37 %. Although shown to be genetically distinct taxa, the two B. mycoides groups exhibited highly similar (98%) 165 RNA sequences. Phylogenetic analyses showed that both B. mycoides and the second group clustered closely with B. cereus. Although not distinguishable by physiological and morphological characteristics, the two B. mycoides groups and B. cereus were clearly separable based on fatty acid composition.