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Sesquiterpene Synthase Gene and Protein Joe Chappell University of Kentucky, [email protected]

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Sesquiterpene Synthase Gene and Protein Joe Chappell University of Kentucky, Chappell@Uky.Edu University of Kentucky UKnowledge Plant and Soil Sciences Faculty Patents Plant and Soil Sciences 9-16-2014 Sesquiterpene Synthase Gene and Protein Joe Chappell University of Kentucky, [email protected] Bryan T. Greenhagen University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits oy u. Follow this and additional works at: https://uknowledge.uky.edu/pss_patents Part of the Plant Sciences Commons Recommended Citation Chappell, Joe and Greenhagen, Bryan T., "Sesquiterpene Synthase Gene and Protein" (2014). Plant and Soil Sciences Faculty Patents. 20. https://uknowledge.uky.edu/pss_patents/20 This Patent is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in Plant and Soil Sciences Faculty Patents by an authorized administrator of UKnowledge. For more information, please contact [email protected]. USOO8835131B2 (12) United States Patent (10) Patent N0.: US 8,835,131 B2 Chappell et a]. (45) Date of Patent: *Sep. 16, 2014 (54) SESQUITERPENE SYNTHASE GENE AND 7,622,614 B2 11/2009 Julien et a1. ................. .. 568/327 PROTEIN 7,790,426 B2 9/2010 Schalk et a1. .. 435/167 8,106,260 B2 1/2012 Chappellet a1. .. .. 800/298 (75) Inventors: Joseph Chappell, Lexington, KY (US); 8,192,950 B2 * 6/2012 Chappell et a1. .. 435/41 _ Bryan Greenhagen’ Belmont, MA 8,354,504 B2 1/2013 Chappelletet a1. .. ... 530/379 (73) Assignee: University of Kentucky Research 8,445,231 B2 5/2013 Chappellet a1. .. 435/691 F°“““‘““°“’ Lexingm’ KY (Us) 3885813822 2‘1 13/5383 Ehaél’e“ " 4152/2/55}? . , ra ey .. (*) Nome: SubJectIO any dlsclalmer, Iheterm 0fth 2003/0203090 A1 10/2003 KOtaChl 61 61. .. 426/489 patent is extended or adjusted under 35 2004/0078840 A1 4/2004 Chappellet a1. .. .. 800/278 U_S_C_ 154(1)) by 225 day, 2005/0210549 A1 9/2005 SChalk 6161. .. 800/287 2006/0218661 A1 9/2006 Chappellet a1. .. .. 800/278 This patent is subject to a terminal dis- 2007/0089198 A1 4/2007 Chappellet a1. .. .. 800/280 Claimer_ 2007/0231861 A1 10/2007 Millis 6161. 435/69.1 2007/0238157 A1 10/2007 Millis et a1. .. 435/166 (21) Appl.No.: 13/385,794 2007/0238159 A1 10/2007 Millis et a1. 435/252.33 2007/0238160 A1 10/2007 Millis et a1. 435/252.33 ' - 2007/0254354 A1 11/2007 Millis et a1. 435/252.33 (22) Flled' Man 6’ 2012 2008/0178354 A1 7/2008 (:11 11 161 800/298 . 2008/0213832 A1 9/2008 Schalkappe et ea1. .. 435/69.1 (65) Pm" Pubhcatlon Data 2008/0233622 A1 9/2008 111116116161. .. 435/148 2010/0035329 A1 2/2010 Millis et a1. 435/254.2 Us 2012/0196340A1 Aug' 2’ 2012 2010/0120110 A1 5/2010 Chappell ..................... .. 435/166 Related US. Application Data (commued) (63) Continuation of application No. 12/259,497, ?led on FOREIGN PATENT DOCUMENTS Oct. 28, 2009, noW Pat. No. 8,192,950, Which is a EP 0036 776 5/1988 continuation of application No. 10/899,356, ?led on Jul. 26, 2004, HOW P211. NO. 7,442,785. EP 0 073 657 12/1990 (60) Provisional application No. 60/489,514, ?led on Jul. OTHEIg P05131151? KTIONS 24’ 2003' Altschul, S., “Amino acid substitution matrices from an information theoretic perspective,” J. M01. Biol. 219(3):555-565 (1991). (51) Int- Cl- ATCC Accession No. CCL 70, derived from CV-l cell line, Deposi C12N9/88 (2006.01) tor: Jensen, F., Retrieved from the Internet:<URL:atcc.org/ATC C12N15/29 (200601) CAdvancedCatalogSearch/ProductDetails/tabid/452/Defau1t.aspx, C12N15/52 (200601) [accessed Feb. 25, 2011; 4 pages]. ATCC Accession No. CCL 163, Depositor: Aaronson, S., Retrieved C12N5/14 (200601) from the Internet:<URL:atcc.org/ATCCAdvancedCatalogSearch/ (52) U-s- Cl- ProductDetails/tabid/452/Defau1taspx, [accessed Feb. 25, 2011; 3 CPC ...................................... .. C12N 9/88 (2013.01) pages]. USPC ...... .. 435/41; 536/231;536/23.6;435/252.3; ATCC Accession N0~ CCL IOTM, DePOSimF MaCPheTSOIl, 16, 4350551. 4356201. 435/348. 435/419 Retrieved from the Internet:<URL:atcc.org/ATC (58) Field of Classi?cation, Search ’ ’ CAdvancedCatalogSearch/ProductDetails/tabid/452/Defau1t.aspx, [accessed Feb. 27, 2011; 3 pages]. None _ _ _ ATCC Accession No. CRL 1651, Cell Type: SV40 transformed, See application ?le for complete search history. Depositor: Gluzman,Y., Retrieved from the Internet: <URL:atcc.0rg/ ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Defau1t. (56) References Cited aSPX, [accessed Fell 25, 2011; 4 Pages} ATCC Accession No. CRL 10478, Depositor: Immunex Corporation, U_S_ PATENT DOCUMENTS Retrieved from the Internet:<URL:atcc.org/ATC CAdvancedCatalogSearch/ProductDetails/tabid/452/Defau1t.aspx, 4,693,905 A 9/1987 Japikse 6161. .............. .. 426/599 [accessed Feb~2512011é4 Pages} 4,973,485 A 11/1990 Rich ............. .. 426/534 C t. d 5,260,086 A 11/1993 Downton 6161. 426/599 ( on “we ) 2’333’313 2‘ 13/1333 E?appell ‘1 ‘1' 28????) Primary Exam/"er * Russell Kan“ 5,847,226, , A 12/1998 Muff;a e et eal‘ a . 568/346 (74) AttorneyiAgein, oerrm. * McKenna Long &Aldr1dge. 5,981,843 A 11/1999 Chappell 6161. 800/301 LLP; Stephame Seldman 6,072,045 A 6/2000 Chappell et a1. .. 536/231 6,468,772 B1 10/2002 Chappell et a1. .. 435/183 (57) ABSTRACT 4 26 /651 The invention relates to sesquiterpene synthases and methods 435/ 183 for their production and use. Particularly, the invention pro 6,495,1936,495,3546,531,3036,559,297 B2B1 12/20023/20035/2003 HiramotoChappelletMillis etal. et a1.a1. 435/ 155 vides nucleic acids comprising the nucleotide sequence of ~~ 536/231 citrus valencene synthase (CVS) Which codes for at least one 6,645,7626,569,656 B2 11/20035/2003 Chappell et al‘a1. 435625435/183 CVS. The invention~ ~ further provides~ nucleic~ a01ds~ compris~ _ 6,689,593 B2 2/2004 Millis et al‘ “““““““““ n 435/155 mg the nucleotide sequence coding for amino a01d residues 6,890,752 B2 5/2005 Chappell et 31, ,,,,,,,,,,,, n 435/325 forming the tier 1 and tier 2 domains of CVS. The invention 7,186,891 B1 3/2007 Chappell et a1. .. 800/298 also provides for methods of making and using the nucleic 7,273,735 B2 9/2007 SChalk et al~ 435/166 acids and amino acids of the current invention. 7,405,057 B2 7/2008 Chappell et a1. .. 435/69.1 7,442,785 B2 * 10/2008 Chappell et a1. ........... .. 536/236 21 Claims, 13 Drawing Sheets US 8,835,131 B2 Page 2 (56) References Cited Craik, C., “Use of oligonucleotides for site-speci?c mutagenesis,” BioTechniques Jan. 12-19, 1985. U.S. PATENT DOCUMENTS Drawert et al., “Regioselective biotransformation of valencene in cell suspension cultures ofCitrus sp.,” Plant Cell Reports 3 :37-40 (1984). 2010/0129306 A1 5/2010 Julien et al. ................... .. 424/65 El Tamer et al., “Domain swapping of Citrus limon monoterpene 2010/0151519 A1 6/2010 Julien et al. 435/69.1 synthases: impact on enzymatic activity and product speci?city,” 2010/0151555 A1 6/2010 Julien et al. ................. .. 435/193 2010/0216186 A1 8/2010 Chappell et al. ........... .. 435/69.1 Arch. Biochem. Biophys. 411:196-203 (2003). 2011/0081703 A1 4/2011 Chappell et al. 435/193 Eyal, E ., “Computer modeling of the enzymatic reaction catalyzed by 2011/0318797 A1 12/2011 Chappell et al. 435/155 5-epi-aristolochene cyclase,” Masters Thesis, Department of Plant 2013/0071877 A1 3/2013 Chappell et al. .............. .. 435/41 Sciences, Weizmann Institute of Science, Rehovot, Israel (Jan. 2001) [44 pages]. FOREIGN PATENT DOCUMENTS Facchini, P. and J. Chappell, “Gene family for an elicitor-induced sesquiterpene cyclase in tobacco,” Proc. Natl. Acad. Sci. U.S.A. EP 1 033 076 9/2000 89: 1 1088-1 1092 (1992). W0 WO 97/38703 10/1997 Fleer et al., “High-level secretion of correctly processed recombinant W0 WO 99/37139 7/1999 W0 WO 00/17327 3/2000 human interleukin-15 in Kluyveromyces lactis,” Gene 107:285-295 W0 W0 02/072758 9/2002 (1991). W0 W0 03/025193 3/2003 GenBank Accession No. AF288465, “Citrus junos terpene synthase W0 WO 2004/031376 4/2004 mRNA, complete cds,” Retrieved from the Internet:<URL:ncbi.nlm. W0 WO 2005/021705 3/2005 nih.gov/nucore/9864188, Published on Aug. 22, 2000. [accessed Feb. W0 WO 2006/079020 7/2006 25,2011] [2 pages]. W0 WO 2010/019696 2/2010 Genbank Accession No. AF411 120 [online], “Citrusxparadisi puta OTHER PUBLICATIONS tive terpene synthase mRNA, complete cds,” Published on Apr. 2, 2002 [retrieved on Feb. 25, 2011] [retrieved from the ATCC Accession No. 37092, Depo sitor: Bernard, H., Retrieved from Internet:<URL:ncbi.nlm.nih.gov/nuccore/AF41 1120] [2 pages]. the Internet: <URL : atcc .org/ATCCAdvancedCatalo gSearch/ GenBank Accession No. AAM00426.1, “putative terpene synthase ProductDetails/tabid/452/Default.aspx, [accessed Apr. 28, 2010; 2 [Citrusxparadisi],” Retrieved from the Internet:<URL:ncbi.nlm.nih. pages]. gov/protein/AAMOO426.1, Published on Apr. 2, 2002. [accessed ATCC Accession No. 53082, Depositors: Immunex Corporation and Feb. 28, 2011] [2 pages]. S.Gillis., Retrieved from the Internet:<URL:atcc.org/ATC GenBank Accession No. AAQ0468.1 (AF441124i1), valencene CAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx, synthase [Citrus sinensis], Retrieved from the Internet:<URL:ncbi. [Apr. 28, 2010; 2 pages]. nlm.nih.gov/protein/33316389, Published on Jan. 12, 2004. Back et al., “Expression of a plant sesquiterpene cyclase gene in [accessed Feb. 25, 2011] [1 page]. Escherichia coli,” Arch. Biochem. Biophys. 315:527-532 (1994). GenBank Accession No. ACK18099, “Sequence 4 from patent US Back, K. and J.
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