SCILOGEX D160/D500 Homogenizers
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Bezanilla Lab Moss Methods Manual
Bezanilla Lab Moss Methods Manual April 2008 Updated July 2012 Contents 1. Tissue Propagation 3 2. Tissue Storage 8 3. Generating Protoplasts 9 4. Transformation 11 5. DNA Isolation 14 6. Protein Isolation 15 7. GUS Assays 17 8. RNA Interference 18 9. 2-Fragment Recombination for N- and -C terminal tagging 23 10. 3-Fragment Recombination for generating knock out constructs 24 11. 4-Fragment Recombination for generating knock in constructs 26 Appendix I – Moss Growth Media Recipes 31 Appendix II – Moss Transformation Reagents 35 Appendix III – Cloning Reactions 38 2 Tissue Propagation Moss tissue should be propagated weekly. For routine culturing of moss, the following unusual items are needed: Cellophanes Tissue homogenizer These items can be acquired from the following sources: Cellophanes: Gel drying cellophanes from Research Products International, catalog #1080 It is ideal to have the cellophanes cut in circles the appropriate size for standard disposable Petri dishes. It is possible to acquire a "hole" punch that will cut out circles of the correct size from the following arts and crafts site: http://www.1stopsquare.com/gigapunc.html Order the Paper Punch Craft, Marvy Uchida Giga - Circle 3". Tissue Homogenizer: Powergen 125 homogenizer – Fisher Scientific 14-261-02 Adaptor for disposable generators – Fisher Scientific 14-261-35 Disposable generators 110 mm in length – Fisher Scientific 14-261-26 These generators can be placed in glass culture tubes, covered with aluminum foil and autoclaved. After use they can be washed in water, air- dried and re-autoclaved. Typically these generators can be re- autoclaved at least 20 times. -
2015 CATALOG the Homogenizer Company™
The Homogenizer Company™ 2015 CATALOG Innovative Solutions Omni International’s homogenizers have been a laboratory staple for over 50 years. Omni sets the indus- try standard with a commitment to outstanding design, performance and a uniquely diversified solution- based product line. We offer a complete portfolio of homogenizers designed for pharmaceutical, life science, biotechnology, agricultural, microbiology and chemical research and development. Our business is strictly homogenizer-focused and we offer the broadest range of solutions to process any sample, including high shear, ultrasonic, mortar and pestle, and bead mill homogenizers. In response to increased activity in global infectious disease research, Omni was first in designing and developing a patented plastic disposable generator probe, the Omni Tip™, which completely eliminates cross-contamination between samples. Our multi-sample and automated homogenizers set the standard by removing the repetitive labor-intensive work of processing samples one at a time. Customer Service Our staff of engineers and technicians are dedicated to finding solutions that improve sample processing, reduce noise, and improve sample processing efficiency, while our support team works to assure that we routinely exceed our customers’ expectations. We are confident that you will find our products and service superior to others. Omni International offers no-risk product evaluations for your convenience. Quality and Convenience Omni products offer the most cost-effective choice for quality, service, and convenience to effectively and ef- ficiently process all types of sample preparation applications, usually in 30 seconds or less. We offer the broadest choice of accessories to process the widest range of samples with user-friendly designs that are easy to clean and maintain. -
Effective Droplet Size Reduction and Excellent Stability of Limonene
colloids and interfaces Article Effective Droplet Size Reduction and Excellent Stability of Limonene Nanoemulsion Formed by High-Pressure Homogenizer Marcel Jonathan Hidajat 1 , Wantaek Jo 2, Hyeonhyo Kim 2 and Jongho Noh 2,* 1 Department of Research and Development, Hiflux Co. Ltd., 361-33, Gapcheon-ro, Daejeon 34037, Korea; [email protected] 2 Department of Research and Development, Ilshin Autoclave Co. Ltd., 255, Techno 2-ro, Daejeon 34026, Korea; jowt@suflux.com (W.J.); hh@suflux.com (H.K.) * Correspondence: njh@suflux.com; Tel.: +82-42-9316100 Received: 18 December 2019; Accepted: 8 January 2020; Published: 10 January 2020 Abstract: Limonene as an interesting bioactive material that has great benefits due to its antimicrobial and anti-carcinogen properties. However, it has several limitations such as its oxidative and oily nature. In order to overcome these limitations, a high-pressure homogenizer (HPH) was utilized to produce limonene nanoemulsion, which enhances its dispersibility while preventing oxidation with great stability. Limonene was pre-mixed with soybean oil as carrier oil prior to emulsification. The effect of soybean oil to limonene ratio, number of pass, homogenization pressure, emulsifier concentration and homogenization method were observed. A stability test was also conducted for 28 days at room temperature. The result revealed that soybean oil and limonene demonstrated a certain ratio to produce the most stable nanoemulsion. Meanwhile, emulsion size could be reduced from 327.8 nm to 55.5 nm in five passes at 1000 bar. Increasing the emulsifier concentration could reduce the droplet size to 40 nm. A comparison with other emulsification method showed that HPH was the best emulsification technique due to its intense emulsification power resulted from shear, cavitation, and droplet impacts. -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout -
WHEATON Media Bottles
> 1 Proven Tools™ for Scientific Research Mission Contact Info WHEATON is a First-Tier Best In Class Global Supplier, a highly effective > USA & Canada ...................................... 800.225.1437 marketer and a product / service innovator serving the general laboratory, life science and diagnostics packaging segments. > International ......................................... 856.825.1100 > Worldwide Fax ...................................... 856.825.1368 Our Life’s work is founded in our unrelenting passion for Customer Satisfaction and Performance Improvement making us incredibly easy to do business with. > Website ..........................................www.wheaton.com Our Associates take pride in our product, our workplace and in performance. > Street ...................................... 1501 North 10th Street Please contact us and our friendly associates will be glad to assist you. > City / State / Zip.....................Millville, NJ 08332-2038 > Country ................................................................USA > Hours ................................ 8:00 a.m. to 5:00 p.m. EST Stephen R. Drozdow President, Chief Executive Officer > 2 Table of Contents Cell Culture Adherent Culture > Incubators * .....................................................................52 > Roller Apparatus * ............................................................51 > Roller Bottles ...................................................................51 > Vented Caps ....................................................................51 -
Food Homogenization
The following is a section of the Elemental Analysis Manual for Food and Related Products. For additional information and to view other sections of the manual, visit the Elemental Analysis Manual for Food and Related Products web page at http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006954.htm. Elemental Analysis Manual for Food and Related Products 2.2 Food Homogenization September, 2014 Authors: William R. Mindak Richard M. Jacobs Stephen G. Capar William C. Cunningham Table of Contents 2.2.1 LABORATORY HOMOGENIZATION EQUIPMENT 2.2.2 HOMOGENIZATION PROCEDURES 2.2.2.1 GENERAL PROCEDURES 2.2.2.2 CANDY PROCEDURES 2.2.2.3 PILLS, CAPSULES, SUPPLEMENTS, ETC. GLOSSARY This section provides information to assist the analyst on physically preparing the analytical sample prior to taking an analytical portion for analysis. Obtaining representative analytical portions is critical for accurate and reproducible results. EAM methods assume the analyst has made the analytical sample homogeneous with respect to the size of the analytical portion. Homogenized foods will vary greatly in texture and viscosity and may separate into liquid and solid components. Analytical samples that have been stored after being homogenized usually require re-homogenization before acquiring an analytical portion. The analyst must carefully observe the physical characteristics of the homogenate and choose an appropriate means of obtaining a representative analytical portion (e.g., pouring directly from container possibly during active mixing, using a 1, 5 or 10 mL pipette or using a spatula). Elemental Analysis Manual (Section 2.2 Food Homogenization) Note: If non-homogeneity is known or suspected then replicate analytical portions should be analyzed and the results assessed. -
2021 Product Guide
2021 PRODUCT GUIDE | LIQUID HANDLING | PURIFICATION | EXTRACTION | SERVICES TABLE OF CONTENTS 2 | ABOUT GILSON 56 | FRACTION COLLECTORS 4 | COVID-19 Solutions 56 | Fraction Collector FC 203B 6 | Service Experts Ready to Help 57 | Fraction Collector FC 204 7 | Services & Support 8 | OEM Capabilities 58 | AUTOMATED LIQUID HANDLERS 58 | Liquid Handler Overview/selection Guide 10 | LIQUID HANDLING 59 | GX-271 Liquid Handler 11 | Pipette Selection Guide 12 | Pipette Families 60 | PUMPS 14 | TRACKMAN® Connected 60 | Pumps Overview/Selection Guide 16 | PIPETMAN® M Connected 61 | VERITY® 3011 18 | PIPETMAN® M 62 | Sample Loading System/Selection Guide 20 | PIPETMAN® L 63 | VERITY® 4120 22 | PIPETMAN® G 64 | DETECTORS 24 | PIPETMAN® Classic 26 | PIPETMAN® Fixed Models 66 | PURIFICATION 28 | Pipette Accessories 67 | VERITY® CPC Lab 30 | PIPETMAN® DIAMOND Tips 68 | VERITY® CPC Process 34 | PIPETMAN® EXPERT Tips 70 | LC Purification Systems 36 | MICROMAN® E 71 | Gilson Glider Software 38 | DISTRIMAN® 72 | VERITY® Oligonucleotide Purification System 39 | REPET-TIPS 74 | Accessories Overview/Selection Guide 40 | MACROMAN® 75 | Racks 41 | Serological Pipettes 43 | PLATEMASTER® 76 | GEL PERMEATION 44 | PIPETMAX® CHROMATOGRAPHY (GPC) 76 | GPC Overview/Selection Guide 46 | BENCHTOP INSTRUMENTS 77 | VERITY® GPC Cleanup System 46 | Safe Aspiration Station & Kit 47 | DISPENSMAN® 78 | EXTRACTION 48 | TRACKMAN® 78 | Automated Extraction Overview/ 49 | Digital Dry Bath Series Selection Guide 49 | Roto-Mini Plus 80 | ASPEC® 274 System 50 | Mini Vortex Mixer 81 | ASPEC® PPM 50 | Vortex Mixer 82 | ASPEC® SPE Cartridges 51 | Digital Mini Incubator 84 | Gilson SupaTop™ Syringe Filters 86 | EXTRACTMAN® 52 | CENTRIFUGES 52 | CENTRY™ 103 Minicentrifuge 88 | SOFTWARE 53 | CENTRY™ 117 Microcentrifuge 88 | Software Selection Guide 53 | CENTRY™ 101 Plate Centrifuge 54 | PERISTALTIC PUMP 54 | MINIPULS® 3 Pump & MINIPULS Tubing SHOP ONLINE WWW.GILSON.COM 1 ABOUT US Gilson is a family-owned global manufacturer of sample management and purification solutions for the life sciences industry. -
(Title of the Thesis)*
In-Situ Continuous Monitoring of Catastrophic Phase Inversion and Viscosity of Pickering Emulsions by Upinder Bains A thesis presented to the University of Waterloo in fulfillment of the thesis requirement for the degree of Master of Applied Science in Chemical Engineering Waterloo, Ontario, Canada, 2018 ©Upinder Bains 2018 AUTHOR'S DECLARATION I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ii Abstract Emulsions are used in the various field such as petroleum, pharmaceuticals, foods, cosmetics, paints, etc. Emulsions stabilized with solid nanoparticles are called Pickering emulsions. More recently, a growing awareness of using environment friendly products has led to more and more researchers to develop and modify natural materials. Starch nanoparticle might be a suitable candidate because they are environmentally friendly, safe and non-toxic. Also, from a practical point of view, starch nanoparticles are low cost, and rheological properties of their emulsions can easily be altered with the help of additives. Because of the unstable nature of these emulsions, continuous agitation is needed to keep the emulsion from separating. This makes the viscosity measurements a challenge, however this task was made possible at low shear rates under some controlled parameters. In this study, an in-situ viscosity measurement method is used to investigate the viscous behaviour of O/W and W/O emulsions at a different volume fraction of the dispersed phase. For this purpose, rotational viscometer installed in a tank was used along with a high shear mixer. -
Gilson Liquid Handling Catalogue 2016-2017 Pipette Selection Guide MECHANICAL PIPETTES PIPETMAN® L & Fixed
Gilson Liquid Handling Catalogue 2016-2017 pipette selection guide MECHANICAL PIPETTES PIPETMAN® L & Fixed ............................................................ 4 - 5 PIPETTES PIPETMAN® L Multi ................................................................. 6 - 7 Choosing the right pipette for your application welcome to Gilson PIPETMAN® G ........................................................................... 8 - 9 PIPETMAN Neo® Single & Multi ........................................10 - 11 PIPETMAN Classic™ & Fixed ..............................................12 - 13 PIPETTE SELECTION GUIDE ELECTRONIC - MOTORISED PIPETTES PIPETMAN® M Single ..........................................................14 - 15 Description Throughput Comfort Features Page PIPETMAN® M Multi............................................................16 - 17 SPECIALITY PIPETTES Reliable, Accurate and Cost Effective Low The original world renowned pipetting standard. 12-13 How To Order PIPETMAN Fixed volume also available. SPECIALITY PIPETTES - Positive Displacement MICROMAN® E ......................................................................18 -19 An Evolution of the Classic Ordering from Gilson has never been MICROMAN®& Capillary Pistons ......................................20 - 21 Lower pipetting and ejection forces PIPETTE ACC Low 10 reduce potential for RSI. easier. You can order online, by phone, fax DISTRIMAN® & REPETMAN® ..............................................22 - 23 or email. SPECIALITY PIPETTES - Large Volume Pipettor Extremely -
Catalog 2005 7.2Mb
These prices are suggested list. Many are discounted. E-mail [email protected] Phone 619-425-1932 Fax 619-425-7917 See www.clarksonlab.com/vHea.htm for discounted prices. Quality Lab Products We are committed to providing highly functional products that work for you in the laboratory. Our innovative ideas come from scientists working every day in labs. We strive to design quality lab products that make processes simpler. Table of Contents Pipetting .........................................2–6 Notebooks...........................................7 Filtering..............................................7 Microscopy...................................8–11 Tube Racks.................................12–20 Tubes.................................................21 Manual Pipette Racks Weighing Boats................................22 Keep pipettes within reach Tubby ® ..............................................23 Four angled compartments array your 0.1 to 25 mL pipettes or cans for easy use. Select the size and type Storage........................................24–25 of pipettes quickly by viewing through the clear acrylic Safety ................................................26 model. The ABS plastic racks feature cut-out windows Magnetized pipette rack Tissue Culture..................................27 for viewing and include two optional attachments that hold pipet bulbs, pipet fillers, and pipet pumps. Liquid Measurement.......................28 Measures 291 x 88 x 401mm. Instrumentation.........................29–31 Magnetic rack has -
BMBL) Quickly Became the Cornerstone of Biosafety Practice and Policy in the United States Upon First Publication in 1984
Biosafety in Microbiological and Biomedical Laboratories 5th Edition U.S. Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention National Institutes of Health HHS Publication No. (CDC) 21-1112 Revised December 2009 Foreword Biosafety in Microbiological and Biomedical Laboratories (BMBL) quickly became the cornerstone of biosafety practice and policy in the United States upon first publication in 1984. Historically, the information in this publication has been advisory is nature even though legislation and regulation, in some circumstances, have overtaken it and made compliance with the guidance provided mandatory. We wish to emphasize that the 5th edition of the BMBL remains an advisory document recommending best practices for the safe conduct of work in biomedical and clinical laboratories from a biosafety perspective, and is not intended as a regulatory document though we recognize that it will be used that way by some. This edition of the BMBL includes additional sections, expanded sections on the principles and practices of biosafety and risk assessment; and revised agent summary statements and appendices. We worked to harmonize the recommendations included in this edition with guidance issued and regulations promulgated by other federal agencies. Wherever possible, we clarified both the language and intent of the information provided. The events of September 11, 2001, and the anthrax attacks in October of that year re-shaped and changed, forever, the way we manage and conduct work -
UVM Cosmogenic Laboratory: Be/Al Extraction
Draft 9: Created on June 15, 2018, be_al_extraction_v9.docx UVM Cosmogenic Laboratory: Be/Al Extraction Purpose: This method details how we extract Be and Al from purified quartz. Hazards: The primary hazard associated with this method is contact with or inhalation exposure to concentrated acids (HF, HCLO4, HCl, HNO3, H2SO4) and beryllium. Personal Protective Gear: Goggles, thin (nitrile) gloves, rubber lab shoes, and lab coat at all times. Add rubber lab smock and face shield when handling HF and HCLO4. Why Blue and Yellow: We work with samples containing a wide range of different 10Be concentrations. To reduce the chance of contaminating low-level samples with material from high-level samples, we maintain two different sets of lab equipment. Low-level samples are processed on yellow lab equipment. High-level samples are processed on blue lab equipment. It is imperative that these two sets of equipment be kept apart and not traded between the processing stations. All labware is clearly color-coded, either on the item itself or on the storage box. Only take labware from the processing stream in which you are working. 1 Draft 9: Created on June 15, 2018, be_al_extraction_v9.docx TEN COMMANDMENTS OF THE UVM COSMOGENIC LABORATORY 1.) Safety is always top priority Approach all of your lab work with this in mind! Understand the safety features of the lab and know how to use them, and know what to do in an emergency. Make sure you are totally comfortable with a procedure before you attempt it. Never perform high-hazard parts of the method when you are alone or tired.