DOCSLIB.ORG

Conformational Switching of Chiral Colloidal Rafts Regulates Raft–Raft Attractions and Repulsions

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Conformational Switching of Chiral Colloidal Rafts Regulates Raft–Raft Attractions and Repulsions Conformational switching of chiral colloidal rafts regulates raft–raft attractions and repulsions Joia M. Millera, Chaitanya Joshia, Prerna Sharmaa,b, Arvind Baskarana, Aparna Baskarana, Gregory M. Grasonc, Michael F. Hagana, and Zvonimir Dogica,d,1 aDepartment of Physics, Brandeis University, Waltham, MA 02454; bDepartment of Physics, Indian Institute of Science, Bangalore 560012, India; cDepartment of Polymer Science and Engineering, University of Massachusetts, Amherst, MA 01003; and dDepartment of Physics, University of California, Santa Barbara, CA 93106 Edited by Monica Olvera de la Cruz, Northwestern University, Evanston, IL, and approved June 19, 2019 (received for review January 25, 2019) Membrane-mediated particle interactions depend both on the membranes limits visualizing the nature of membrane-mediated properties of the particles themselves and the membrane envi- interactions and associated assembly pathways (17–26). ronment in which they are suspended. Experiments have shown Tunable depletion interactions enable the robust assembly that chiral rod-like inclusions dissolved in a colloidal membrane of monodisperse rod-like molecules into colloidal monolayer of opposite handedness assemble into colloidal rafts, which are membranes, structures that mimic many of the properties of the finite-sized reconfigurable droplets consisting of a large but pre- lipid bilayers yet are about 2 orders of magnitude larger (27–30). cisely defined number of rods. We systematically tune the chirality The 1-μm-thick colloidal membrane allows for direct visualization of the background membrane and find that, in the achiral limit, of inclusions within the membrane and the associated environ- colloidal rafts acquire complex structural properties and interac- mental deformations. For example, recent experiments have shown tions. In particular, rafts can switch between 2 chiral states of op- that chiral inclusions dissolved in a colloidal membrane of opposite posite handedness, which alters the nature of the membrane- chirality assemble into colloidal rafts, which are micrometer-sized – mediated raft raft interactions. Rafts with the same chirality have deformable liquid droplets consisting of a large but precisely de- long-ranged repulsions, while those with opposite chirality acquire fined number of rods (31–33). Here, we show that colloidal rafts attractions with a well-defined minimum. Both attractive and repul- can assemble even in achiral membranes and that such rafts can SCIENCES sive interactions are qualitatively explained by a continuum model switch between 2 distinct chiral states. Furthermore, we show that APPLIED PHYSICAL that accounts for the coupling between the membrane thickness interconverting between these 2 conformational states switches the and the local tilt of the constituent rods. These switchable interac- effective raft–raft pair interaction from a long-ranged repulsion to tions enable assembly of colloidal rafts into intricate higher-order a short-ranged attraction. These conformationally switchable self- architectures, including stable tetrameric clusters and “ionic crystal- assembled mesoscopic structures and interactions drive formation lites” of counter-twisting domains organized on a binary square lattice. Furthermore, the properties of individual rafts, such as their of diverse higher-order reconfigurable assemblages. Using the- sizes, are controlled by their complexation with other rafts. The oretical modeling, we show that the complex interaction behav- emergence of these complex behaviors can be rationalized purely ior can be explained by considering the coupling between the in terms of generic couplings between compositional and orienta- shape of raft interfaces and the tilt field of colloidal rods within tional order of fluids of rod-like elements. Thus, the uncovered prin- the membrane. We find that a minimal symmetry-based model of ciples might have relevance for conventional lipid bilayers, in which phase-separated and tilted membranes qualitatively describe most the assembly of higher-order structures is also mediated by complex membrane-mediated interactions. Significance self-assembly | membranes | liquid crystals | colloids We describe hierarchical assemblages of colloidal rods that mimic some of the complexity and reconfigurability of bi- hen suspended in a bulk isotropic liquid, colloids interact ological structures. We show that chiral rod-like inclusions Wby forces that have steric, electrostatic, or entropic origins dissolved in an achiral colloidal membrane assemble into rafts, (1). In comparison, the same particles suspended in an aniso- which are adaptable finite-sized liquid droplets that exhibit tropic liquid crystals, or at a fluid interface, distort their envi- 2 distinct chiral states of opposite handedness. Interconverting ronment and thus acquire fundamentally different interactions. between these 2 states switches the membrane-mediated raft interactions between long-ranged repulsions and attractions. For example, colloids in a liquid crystal alter the nematic director Rafts with switchable interactions assemble into analogs of field, generating topological defects, which lead to unique long- electrostatic complexation observed in charged particulate range interactions that drive the assembly of complex architec- matter. Our results demonstrate a robust pathway for self- – – tures (2 5). Colloids bound to oil water interfaces provide an- assembly of reconfigurable colloidal superstructures that does other example in which long-ranged attractive or repulsive not depend on tuning the shape and interactions of the ele- interactions are caused by the interface deformations. These in mental units, but rather on the complexity of the emergent turn are determined by how the interface wets the colloids (6, 7). membrane-mediated interactions. Lipid membranes provide a complex environment that combines both liquid crystalline-like anisotropy due to the alignment of the Author contributions: J.M.M. and Z.D. designed research; J.M.M., C.J., and Arvind Baskaran performed research; P.S. acquired preliminary data; J.M.M., C.J., Arvind Baskaran, Aparna fatty chains and interface deformations due to effective 2D Baskaran, and G.M.G. analyzed data; and J.M.M., M.F.H., and Z.D. wrote the paper. confinement (8). Inclusions distort both the membrane thickness The authors declare no conflict of interest. and the chain alignment, leading to interactions that are even This article is a PNAS Direct Submission. more multifaceted than those observed for colloids in liquid Published under the PNAS license. – crystals or on interfaces (9 12). For example, even inclusions 1To whom correspondence may be addressed. Email: [email protected]. with purely repulsive interactions may form stable clusters due to This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. the interplay of overlapping regions of membrane deformation in 1073/pnas.1900615116/-/DCSupplemental. lipid bilayers (13–16). However, the nanometer size of lipid www.pnas.org/cgi/doi/10.1073/pnas.1900615116 PNAS Latest Articles | 1of10 Downloaded by guest on September 30, 2021 of the experimental observations. Our results demonstrate how mains of either handedness have the same spacing. This criterion properties of the membrane environment can be tuned to generate reveals that colloidal membranes composed of 63% Y21M and complex self-assemblages from structurally simple building blocks. 37% wt rods are effectively achiral. Based on these findings, we define the chiral parameter: ϕch = ðrM13KO7 − 0.37Þ=0.63, where Assembly of Rafts in Achiral Colloidal Membranes rM13KO7 = nM13KO7=ðnM13KO7 + nM13KO7−Y21M Þ, is the fraction of fd We used M13KO7 and bacteriophage, which are both rod-like left-handed rods, and nM13KO7 and nM13KO7−Y21M are the concen- colloidal particles with ∼7-nm diameter and 2.8-μm persistence trations of long rods. For achiral mixtures, ϕch = 0,while ϕch = 1 length (34). M13KO7 is 1,200 nm long, while fd-wt is 880 nm indicates maximally left-handed rod composition. Achiral colloidal long. Addition of a nonadsorbing polymer to a virus solution membranes composed of fd-wt and fd-Y21M exhibit an edge in- induces attractive interactions, causing the viruses to assemble stability that is consistent with the absence of rod twist (40). into colloidal membranes, which are liquid-like monolayers of Colloidal membranes comprised of a uniform mixture of rods rods, aligned lengthwise, with lateral dimensions reaching hun- of opposite chirality, such as M13KO7 and fd-Y21M, force the dreds of microns (27, 28). Local twisting of rods as preferred by constituents within the membrane interior to untwist. However, their intrinsic chirality is fundamentally incompatible with the rods with opposite chirality and different lengths can form chiral global constraints of the 2D membrane geometry. Consequently, colloidal rafts that allow the twist to penetrate the membrane all of the rods within the membrane interior are forced into a interior and thus lower the system free energy. These colloidal higher-energy untwisted state, while rods within a twist pene- rafts are finite-sized equilibrium droplets of 1-handedness and tration length of the membrane edge are free to twist and thus length that coexist with the background membrane of the op- lower their energy (35). This frustration leads to chiral control of posite handedness and different thickness (31). The finite twist at the membrane edge tension (36). a raft’s edge decays
Load more

Recommended publications
  • THE LAST RAFT As It Appeared to a Contemporary
    THE LAST RAFT As It Appeared to a Contemporary By LEWIS EDWIN THEISS W ITH the highly commendable purpose of giving the present generation a glimpse of a phase of the life of the past, Mr. R. Dudley Tonkin, a lumber operator of Tyrone, Pa., with his brother, C. Ord Tonkin, in the spring of 1938 constructed the Last Raft. The craft was put together at Burnside, above -iMc- Gee's Mills, far up the West Branch of the Susquehanna, and was to be floated to Harrisburg, approximately 200 miles dis- tant. There, through the cooperation of J. D. Bogar, Jr., a Har- risburg lumiber dealer, the logs would be purchased at the end of the journey. Thousands of persons would have an opportunity to see this log raft-the first to go down the river since 1912, when commercial log rafting came to an end. It was said, too, that Mr. Tonkin's effort was also to celebrate the centennial of his grandfather's first voyage down the river on a log raft. However that may be, this was truly an effort to resurrect the past; for not only would the raft be constructed exactly as log rafts had been fashioned for generations, but the men who would operate it were old time raftmen. Necessarily they were men of advanced years. Seldom has any effort along historical lines stirred up such tremendous interest. Here was to be no static picture of the past, carefully posed behind glass display windows, but an actual exhibition of the real thing-a bit of the past come to life.
    [Show full text]
  • Download the Basketballplayer.Ngql Fle Here
    Nebula Graph Database Manual v1.2.1 Min Wu, Amber Zhang, XiaoDan Huang 2021 Vesoft Inc. Table of contents Table of contents 1. Overview 4 1.1 About This Manual 4 1.2 Welcome to Nebula Graph 1.2.1 Documentation 5 1.3 Concepts 10 1.4 Quick Start 18 1.5 Design and Architecture 32 2. Query Language 43 2.1 Reader 43 2.2 Data Types 44 2.3 Functions and Operators 47 2.4 Language Structure 62 2.5 Statement Syntax 76 3. Build Develop and Administration 128 3.1 Build 128 3.2 Installation 134 3.3 Configuration 141 3.4 Account Management Statement 161 3.5 Batch Data Management 173 3.6 Monitoring and Statistics 192 3.7 Development and API 199 4. Data Migration 200 4.1 Nebula Exchange 200 5. Nebula Graph Studio 224 5.1 Change Log 224 5.2 About Nebula Graph Studio 228 5.3 Deploy and connect 232 5.4 Quick start 237 5.5 Operation guide 248 6. Contributions 272 6.1 Contribute to Documentation 272 6.2 Cpp Coding Style 273 6.3 How to Contribute 274 6.4 Pull Request and Commit Message Guidelines 277 7. Appendix 278 7.1 Comparison Between Cypher and nGQL 278 - 2/304 - 2021 Vesoft Inc. Table of contents 7.2 Comparison Between Gremlin and nGQL 283 7.3 Comparison Between SQL and nGQL 298 7.4 Vertex Identifier and Partition 303 - 3/304 - 2021 Vesoft Inc. 1. Overview 1. Overview 1.1 About This Manual This is the Nebula Graph User Manual.
    [Show full text]
  • Pilot Stories
    PILOT STORIES DEDICATED to the Memory Of those from the GREATEST GENERATION December 16, 2014 R.I.P. Norm Deans 1921–2008 Frank Hearne 1924-2013 Ken Morrissey 1923-2014 Dick Herman 1923-2014 "Oh, I have slipped the surly bonds of earth, And danced the skies on Wings of Gold; I've climbed and joined the tumbling mirth of sun-split clouds - and done a hundred things You have not dreamed of - wheeled and soared and swung high in the sunlit silence. Hovering there I've chased the shouting wind along and flung my eager craft through footless halls of air. "Up, up the long delirious burning blue I've topped the wind-swept heights with easy grace, where never lark, or even eagle, flew; and, while with silent, lifting mind I've trod the high untrespassed sanctity of space, put out my hand and touched the face of God." NOTE: Portions Of This Poem Appear On The Headstones Of Many Interred In Arlington National Cemetery. TABLE OF CONTENTS 1 – Dick Herman Bermuda Triangle 4 Worst Nightmare 5 2 – Frank Hearne Coming Home 6 3 – Lee Almquist Going the Wrong Way 7 4 – Mike Arrowsmith Humanitarian Aid Near the Grand Canyon 8 5 – Dale Berven Reason for Becoming a Pilot 11 Dilbert Dunker 12 Pride of a Pilot 12 Moral Question? 13 Letter Sent Home 13 Sense of Humor 1 – 2 – 3 14 Sense of Humor 4 – 5 15 “Poopy Suit” 16 A War That Could Have Started… 17 Missions Over North Korea 18 Landing On the Wrong Carrier 19 How Casual Can One Person Be? 20 6 – Gardner Bride Total Revulsion, Fear, and Helplessness 21 7 – Allan Cartwright A Very Wet Landing 23 Alpha Strike
    [Show full text]
  • Annotated Books Received
    ANNOTATED BOOKS RECEIVED EDITOR'S NOTE: ANTHOLOGIES In 1983 when Translation Review began its "Annotated Books Received," approximately 60 publishers were represented. Over the years, the publishing of (French) A Flea in Her Rear (or Ants in Her Pants) and other translations has become more widespread and Translation Vintage French Farces. Tr. Norman R. Shapiro. Applause Review's contacts with publishers more numerous. The Books. 1994. 479 pp. Paper: $15.95; ISBN 1-55783-165-3. journal celebrates both that growth and those contacts with "Replete with mistaken identities, concealments and sudden this first issue of a separate "Annotated Books Received revelations, jack-in-the-box irruptions, physical disorder, and assaults on logic, both situational and linguistic..." [N.S.] the Supplement," in which almost 100 publishers are plays in this collection are such noted farces as "The Castrata," represented. This listing of books sent to Translation "Signor Nicodemo," "Boubouroche, or She Dupes to Review will be published twice each year. Conquer," "A Flea in Her Rear, or Ants in Her Pants," and "For Love or Monkey." Shapiro won the 1992 ALTA Outstanding Two primary reasons for the new publication are space Translation Award for his translation of The Fabulists French. and convenience. The "Annotated Books Received" section in regular issues of Translation Review has grown (Arabic) Arabic Short Stories. Tr. Denys Johnson-Davies. to the point of dominating issue space. This new University of California Press. 1994. 216 pp. Cloth: $32.00; supplement will allow more critical discussion and reviews ISBN 0-520-08563-9. Paper: $12.00; ISBN 0-520-08944-8.
    [Show full text]
  • SAT I: Reasoning Test
    SAT I: Reasoning Test Saturday, May 2002 1 YOUR NAME (PRINT) LAST FIRST MI TEST CENTER NUMBER NAME OF TEST CENTER ROOM NUMBER SAT ® I: Reasoning Test — General Directions Timing IMPORTANT: The codes below are unique to your •You will have three hours to work on this test. test book. Copy them on your answer sheet in boxes 8 • There are five 30-minute sections and two 15-minute sections. and 9 and fill in the corresponding ovals exactly as •You may work on only one section at a time. shown. • The supervisor will tell you when to begin and end each section. • If you finish a section before time is called, check your work on that 8. Form Code section. You may NOT turn to any other section. •Work as rapidly as you can without losing accuracy. Don't waste time on questions that seem too difficult for you. A A 0 0 0 Marking Answers B B 1 1 1 • Carefully mark only one answer for each question. C C 2 2 2 • Make sure each mark is dark and completely fills the oval. D D 3 3 3 • Do not make any stray marks on your answer sheet. E E 4 4 4 • If you erase, do so completely. Incomplete erasures may be scored as intended answers. F F 5 5 5 •Use only the answer spaces that correspond to the question G G 6 6 6 numbers. H H 7 7 7 • For questions with only four answer choices, an answer marked in I I 8 8 8 oval E will not be scored.
    [Show full text]
  • The Drink Tank Sixth Annual Giant Sized [email protected]: James Bacon & Chris Garcia
    The Drink Tank Sixth Annual Giant Sized Annual [email protected] Editors: James Bacon & Chris Garcia A Noise from the Wind Stephen Baxter had got me through the what he’ll be doing. I first heard of Stephen Baxter from Jay night. So, this is the least Giant Giant Sized Crasdan. It was a night like any other, sitting in I remember reading Ring that next Annual of The Drink Tank, but still, I love it! a room with a mostly naked former ballerina afternoon when I should have been at class. I Dedicated to Mr. Stephen Baxter. It won’t cover who was in the middle of what was probably finished it in less than 24 hours and it was such everything, but it’s a look at Baxter’s oevre and her fifth overdose in as many months. This was a blast. I wasn’t the big fan at that moment, the effect he’s had on his readers. I want to what we were dealing with on a daily basis back though I loved the novel. I had to reread it, thank Claire Brialey, M Crasdan, Jay Crasdan, then. SaBean had been at it again, and this time, and then grabbed a copy of Anti-Ice a couple Liam Proven, James Bacon, Rick and Elsa for it was up to me and Jay to clean up the mess. of days later. Perhaps difficult times made Ring everything! I had a blast with this one! Luckily, we were practiced by this point. Bottles into an excellent escape from the moment, and of water, damp washcloths, the 9 and the first something like a month later I got into it again, 1 dialed just in case things took a turn for the and then it hit.
    [Show full text]
  • Einstein, History, and Other Passions : the Rebellion Against Science at the End of the Twentieth Century
    Einstein, history, and other passions : the rebellion against science at the end of the twentieth century The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Holton, Gerald James. 2000. Einstein, history, and other passions : the rebellion against science at the end of the twentieth century. Cambridge, MA: Harvard University Press. Published Version http://www.hup.harvard.edu/catalog.php?isbn=9780674004337 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:23975375 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA EINSTEIN, HISTORY, ANDOTHER PASSIONS ;/S*6 ? ? / ? L EINSTEIN, HISTORY, ANDOTHER PASSIONS E?3^ 0/" Cf72fM?y GERALD HOLTON A HARVARD UNIVERSITY PRESS C%772^r?<%gf, AizziMc^zzyeZZy LozzJozz, E?zg/%??J Q AOOO Many of the designations used by manufacturers and sellers to distinguish their products are claimed as trademarks. Where those designations appear in this book and Addison-Wesley was aware of a trademark claim, the designations have been printed in capital letters. PHYSICS RESEARCH LIBRARY NOV 0 4 1008 Copyright @ 1996 by Gerald Holton All rights reserved HARVARD UNIVERSITY Printed in the United States of America An earlier version of this book was published by the American Institute of Physics Press in 1995. First Harvard University Press paperback edition, 2000 o/ CoMgre.w C%t%/og;Hg-zM-PMMt'%tz'c7t Dzztzz Holton, Gerald James.
    [Show full text]
  • JOURNEY to the CENTER of the EARTH by DIANA MITCHELL
    A TEACHER’S GUIDE TO THE SIGNET CLASSIC EDITION OF JULES VERNE’S JOURNEY TO THE CENTER OF THE EARTH By DIANA MITCHELL SERIES EDITORS: W. GEIGER ELLIS, ED.D., UNIVERSITY OF GEORGIA, EMERITUS and ARTHEA J. S. REED, PH.D., UNIVERSITY OF NORTH CAROLINA, RETIRED A Teacher’s Guide to the Signet Classic Edition of Jules Verne’s Journey to the Center of the Earth 2 INTRODUCTION Journey to the Center of the Earth by Jules Verne is a novel that literally plunges the reader into the center of the earth through vivid description, detailed explanations, and the “eyewitness” accounts of the narrator. On the most basic level, Journey is an adventure story—a tale of the obstacles, encounters, and wonders. The eccentric scientist Professor Hardwigg finds directions to the center of the earth in an old book and sets out, along with his nephew Henry and the guide Hans, to Iceland where they find the mountain and the shaft that allows them access to the depths of the earth. On a deeper level the story can be seen as man’s journey into himself, always probing deeper for what lies at his center. Written in 1864 this novel is a remarkable look into the future. Although students will recognize scientific predictions that were based on inaccurate assumptions, language that is somewhat antiquated, and a beginning that proceeds at a leisurely pace, they will appreciate Verne’s ability to weave into the story information and questions about science that will keep them in a state of curiosity and wonderment.
    [Show full text]
  • Improving Our Ability to Estimate Vital Rates of Endangered Fishes on the San Juan River Using Novel Applications of PIT-Tag Technology
    Utah State University DigitalCommons@USU All Graduate Theses and Dissertations Graduate Studies 8-2020 Improving Our Ability to Estimate Vital Rates of Endangered Fishes on the San Juan River Using Novel Applications of PIT-Tag Technology J. Benjamin Stout Utah State University Follow this and additional works at: https://digitalcommons.usu.edu/etd Part of the Terrestrial and Aquatic Ecology Commons Recommended Citation Stout, J. Benjamin, "Improving Our Ability to Estimate Vital Rates of Endangered Fishes on the San Juan River Using Novel Applications of PIT-Tag Technology" (2020). All Graduate Theses and Dissertations. 7824. https://digitalcommons.usu.edu/etd/7824 This Thesis is brought to you for free and open access by the Graduate Studies at DigitalCommons@USU. It has been accepted for inclusion in All Graduate Theses and Dissertations by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. IMPROVING OUR ABILITY TO ESTIMATE VITAL RATES OF ENDANGERED FISHES ON THE SAN JUAN RIVER USING NOVEL APPLICATIONS OF PIT-TAG TECHNOLOGY by J. Benjamin Stout A thesis submitted in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in Ecology Approved: ______________________ _______________________ Phaedra Budy, Ph. D. Mary M. Conner, Ph.D. Major Professor Committee Member ______________________ _______________________ Mark C. McKinstry, Ph.D. Richard S. Inouye, Ph.D. Committee Member Vice Provost for Graduate Studies UTAH STATE UNIVERSITY Logan, Utah 2020 ii Copyright © J. Benjamin Stout 2020 All Rights Reserved iii ABSTRACT Improving Our Ability to Estimate Vital Rates of Endangered Fishes on the San Juan River Using Novel Applications of Pit-Tag Technology by J.
    [Show full text]
  • UNIVERSITY of ARIZONA PRESS SPRING 2019 the University of Arizona Press Is the Premier Publisher of Academic, Regional, and Literary Works in the State of Arizona
    Celebrating 60 Years THE UNIVERSITY OF ARIZONA PRESS SPRING 2019 The University of Arizona Press is the premier publisher of academic, regional, and literary works in the state of Arizona. We disseminate ideas and knowledge of lasting value that enrich understanding, inspire curiosity, and enlighten readers. We advance the University of Arizona’s mission by connecting scholarship and creative expression to readers worldwide. www.uapress.arizona.edu CONTENTS AFRICAN AMERICAN STUDIES, 11 ANTHROPOLOGY, 20, 21, 22, 23, 24, 25, 31, 33 ARCHAEOLOGY, 29, 30, 31, 32, 33 BIOGRAPHY, 6 BORDER STUDIES, 15, 22, 25 ENVIRONMENTAL STUDIES, 8, 9, 24 HISTORY, 15, 16, 17 INDIGENOUS STUDIES, 2, 5, 18, 19, 20, 21, 22, 23, 26, 27 LATIN AMERICAN STUDIES, 17, 19, 23, 26, 27, 28 LATINX STUDIES, 3, 4, 10, 12, 13, 14, 15, 16, 21 NATURE, 8, 9 POETRY, 2, 3, 4, 5 SOCIAL JUSTICE, 10, 11 SPACE SCIENCE, 6, 7 RECENTLY PUBLISHED, 34–37 RECENT BEST SELLERS, 38–43 SALES INFORMATION, INSIDE BACK COVER CATALOG DESIGN BY LEIGH MCDONALD COVER PHOTOS: FROM STORM IN THE DESERT BY CEBIMAGERY [FRONT]; OPENING TO CANYON DE CHELLY, NAVAJO INDIAN RESERVATION, ARIZONA, CA. 1900 BY CHARLES C. PIERCE, DIGITALLY REPRODUCED BY THE USC DIGITAL LIBRARY; FROM THE CALIFORNIA HISTORICAL SOCIETY COLLECTION AT THE UNIVERSITY OF SOUTHERN CALIFORNIA [INSIDE] PROUD MEMBER OF AUPRESSES BROTHER BULLET POEMS CASANDRA LÓPEZ A vivid response to love and loss, trauma and survival Speaking to both a personal and collective loss, in Brother Bullet Casandra López confronts her relationships with violence, grief, guilt, and ultimately, endurance. Revisiting the memory and lasting consequences of her brother’s murder, López traces the course of the bullet—its trajectory, impact, wreck- age—in lyrical narrative poems that are haunting and raw with emotion, yet tender and alive in revelations of light.
    [Show full text]
  • The Manifold Trilogy, Book 1) Pdf, Epub, Ebook
    TIME (THE MANIFOLD TRILOGY, BOOK 1) PDF, EPUB, EBOOK Stephen Baxter | 464 pages | 22 Oct 2015 | HarperCollins Publishers | 9780008134464 | English | London, United Kingdom Time (the Manifold Trilogy, Book 1) PDF Book Is he a prophet? End of story. I recommend it. I seem to have had a similar experience to many who have struggled doggedly through Stephen Baxter's novels: the ideas he presents generally hard science in the form of current theoretical physics, mathematics, bioengineering, etc. Readers also enjoyed. But this is where it starts to get weird. This is a fundamental change in the structure of the universe. Well worth your time. This statistical doomsday argument is not only counter intuitive, it is also completely bogus. Error rating book. Related Articles. Want your brain twisted with some crazy hard science? Used this way the model makes a set of independent predictions on consecuitive time steps. Try "Flood" if you like near-future thrillers. Details if other :. For a short story set in the universe of this novel, click here. His novel Voyage won the Sidewise Award for Best Alternate History Novel of the Stephen Baxter is a trained engineer with degrees from Cambridge mathematics and Southampton Universities doctorate in aeroengineering research. News Click the links below for more details:. Sprinkle with stale dust of Ayn Rand's Far right Technocracy and add a tiny drop of Essence of God - just a whiff makes all the difference. Structured data. This is likely erroneous. The last column of the data, wd deg , gives the wind direction in units of degrees.
    [Show full text]
  • Ellsworth American—Only Paper
    Voi- XLV. )sy,"«,'^?^"c,yVig,.-BaneocH Co ah GLL8WORTII, MAINE, WEDNESDAY AFTERNOON, JULY 12, 1899. J'*T;?ul£JZS?%V$Sn,"Zrai No. 28 abprttiermrntfl. atibertiBementss. LOCAL AFFAIRS. can, while Editor Drinkwnter was in the legislature at Augusta. Prof. Sampson NEW ADVERTISEMENTS THIS WEEK. got newspaper experience enough to Inst C. 6 HURRILL & him a life-time, and went hack to teach- SON™ In bankruptcy—K«t Chan U Runs. — Prof. Probate tiotlc'- Kata Melinda B Cnndagc et ing. Sampson left Ellsworth als. Thursday to attend the American Insti- Insolvency noth- K-t Kiank W Glle.- et al. tute of instruction at liar Harbor. ®AKINfr notice—K-t B 1 Kxec Walter Blalsdell. I 1 I \ I /*VAl. who attended dohn A Hale—Bunk store for Among Ellsworth people Bithti.i, Bank ami stationery Bt.dg., ELLSWORTH, ME. sale. rao-tings of the American Institute of pow^-^ M Gallert —l>ry goods, Instruction at liar Harbor the'1 ^ d A < ii i) id,Nirb:',m •'..•tlojicr during Absolutely pure wK KKPKBAKMT TUB A W Cushman A Sou—Furniture. past week were Hoyt A. Moore, Ernest L. Moni Reliable Home ami Bangor Moore, Elmer F. Mureh, Mrs. II. C. Makes the food more delicious and wholesome Foreign Companies. Bangor business college. Miss M. A. and Hntheway, Greely Miss ROVAt BAKING POWDER NEW VORK. Tvlcr, Fogg .V. < u bonds. CO., Rates with -Municipal Minnie Moore. P—WP—1———————J——i—g——TXII 'HKST——A tm (Jompeitit)le Safety. Blake, Barrows ,V Brow u— Investment se- curities. Friday afternoon at 2.30 at the Free Portland■ in aumu to suit on improved real estate am ! Baptist church a council appointed by the members of the Sunday school will be .NEW I>001vt5.
    [Show full text]