Objectives:

PLANT TISSUES ศึกษาเนื้อเยื่อชนิดตางๆ ของพืช เนื้อเยอพื่ ืช ศึกษาตําแหนงและการจัดเรียงตัว PLANT TISSUES ของเนื้อเยื่อและเซลลแตละชนิดในพืช ศึกษาเปรียบเทียบลกษณะทั ี่แตกตาง กันของพืชใบเลี้ยงเดี่ยวและพืชใบเลี้ยงคู Dr. Boonsatien Boonsoong

Department of Zoology ฝกการเตรียมสไลดสดโดยวิธี Faculty of Science free hand section Kasetsart University

Plant growth: Stem growth:

primary growth secondary growth

apical meristem lateral meristem

differentiate

primary tissue vascular cambium cork cambium

• epidermis differentiate differentiate •cortex • primary xylem • secondary xylem periderm • primary phloem • secondary phloem (cork, phelloderm) Plant growth: Plant tissues: cell division

• Meristematic tissue

• Apical meristem

• Lateral meristem: vascular cambium, cork cambium

• Intercalary meristem

• Permanent tissue

• Simple permanent tissue: epidermis, cork, parenchyma, collenchyma, sclerenchyma

• Complex permanent tissue: xylem, phloem

Meristematic tissue: Meristematic tissue:

Apical meristem Apical meristem

shoot apex root apex Meristematic tissue: Meristematic tissue:

Lateral meristem Lateral meristem (cont.)

• Vascular cambium - สราง secondary xylem • Cork cambium - สราง cork และ phelloderm และ secondary phloem - Dicot

Meristematic tissue: Permanent tissue:

Intercalary meristem Simple

Epidermis

Cork

Parenchyma

Collenchyma

Sclerenchyma Simple permanent tissue: Simple permanent tissue:

Epidermis Epidermis ƒ dermal tissue, single layer of cells •secretesa waxylayercalledthe cuticle that inhibits water loss

guard cell

Simple permanent tissue: Simple permanent tissue:

Cork Parenchyma ƒ non-living, water-resistant • healing and tissue regeneration • protective tissue that displaces the stem epidermis • photosyntesis intercellular • food storage air space

Parenchyma Chlorenchyma Simple permanent tissue: Simple permanent tissue:

Parenchyma Collenchyma • unevenly thick primary cell wall palisade cell • support in young stems, roots, and petrioles

spongy cell

Transverse section of leaf of common barberry (Berberis vulgaris) Transverse section of stem of angelica

Simple permanent tissue: Simple permanent tissue:

Collenchyma cells Sclerenchyma

Celery (Apium) • dead at maturity • support and protection • secondary cell wall C.S.

L.S.

X-section L-section Simple permanent tissue: Simple permanent tissue: Sclerenchyma Sclereid Fiber Sclereid

Pear (Pyrus)

Permanent tissue: Complex permanent tissue:

Simple Complex Vessel member Epidermis

Xylem XYLEM Cork Tracheid • Conduct water Phloem and dissolved Parenchyma Xylem fiber minerals • Support Collenchyma Xylem parenchyma Sclerenchyma Xylem: Complex permanent tissue:

Vessel Tracheid PHLOEM

• conduct food and other organic substances

Phloem Phloem Sieve tube Companion fiber parenchyma member cell • dead at maturity • end wall - imperforation • end wall - perforation • gymnosperm • angiosperm

Phloem: Monocot & Dicot:

Sieve tube Companion cell

•sieve plate Monocot & Dicot: Stem monocot:

Monocot stem, c.s. Dicot stem, c.s. X - section

Stem monocot: Stem dicot:

Vascular tissue Epidermis

Cortex

Vascular bundle

Pith

X - section X - section Stem dicot: Stem dicot:

Epidermis

Cortex

Pith

Phloem

Xylem

Stem dicot: Lab. session:

Vascular tissue Permanent Slide

Meristem tissue Permanent tissue Monocot & Dicot • L-S), Allium sp. • Epidermis • Compare root • Periderm • X-S, corn stem •X-S, Coleus • Collenchyma •X-S, Coleus sp. sp. stem stem • Sclerenchyma • Vascular bundle • Parenchyma • Fiber • Chlorenchyma

X - section Free hand sectioning: Lab. results:

Fiber ของวานฝกดาบ

Wet mount

•X-S, หญาละออง

•X-S, วานฝกดาบ

•X-S, ฝรั่ง

• กะลามะพราว • Safranin dye Safranin stained Aniline sulfate stained • Aniline sulfate

Lab. results: Lab. results:

X-S หญาละออง Sclerenchyma ของฝรั่ง

Vascular tissue, safranin stained Sclereid, safranin stained