Objectives: PLANT TISSUES ศึกษาเนื้อเยื่อชนิดตางๆ ของพืช เนื้อเยอพื่ ืช ศึกษาตําแหนงและการจัดเรียงตัว PLANT TISSUES ของเนื้อเยื่อและเซลลแตละชนิดในพืช ศึกษาเปรียบเทียบลกษณะทั ี่แตกตาง กันของพืชใบเลี้ยงเดี่ยวและพืชใบเลี้ยงคู Dr. Boonsatien Boonsoong Department of Zoology ฝกการเตรียมสไลดสดโดยวิธี Faculty of Science free hand section Kasetsart University Plant growth: Stem growth: primary growth secondary growth apical meristem lateral meristem differentiate primary tissue vascular cambium cork cambium • epidermis differentiate differentiate •cortex • primary xylem • secondary xylem periderm • primary phloem • secondary phloem (cork, phelloderm) Plant growth: Plant tissues: cell division • Meristematic tissue • Apical meristem • Lateral meristem: vascular cambium, cork cambium • Intercalary meristem • Permanent tissue • Simple permanent tissue: epidermis, cork, parenchyma, collenchyma, sclerenchyma • Complex permanent tissue: xylem, phloem Meristematic tissue: Meristematic tissue: Apical meristem Apical meristem shoot apex root apex Meristematic tissue: Meristematic tissue: Lateral meristem Lateral meristem (cont.) • Vascular cambium - สราง secondary xylem • Cork cambium - สราง cork และ phelloderm และ secondary phloem - Dicot Meristematic tissue: Permanent tissue: Intercalary meristem Simple Epidermis Cork Parenchyma Collenchyma Sclerenchyma Simple permanent tissue: Simple permanent tissue: Epidermis Epidermis dermal tissue, single layer of cells •secretesa waxylayercalledthe cuticle that inhibits water loss guard cell Simple permanent tissue: Simple permanent tissue: Cork Parenchyma non-living, water-resistant • healing and tissue regeneration • protective tissue that displaces the stem epidermis • photosyntesis intercellular • food storage air space Parenchyma Chlorenchyma Simple permanent tissue: Simple permanent tissue: Parenchyma Collenchyma • unevenly thick primary cell wall palisade cell • support in young stems, roots, and petrioles spongy cell Transverse section of leaf of common barberry (Berberis vulgaris) Transverse section of stem of angelica Simple permanent tissue: Simple permanent tissue: Collenchyma cells Sclerenchyma Celery (Apium) • dead at maturity • support and protection • secondary cell wall C.S. L.S. X-section L-section Simple permanent tissue: Simple permanent tissue: Sclerenchyma Sclereid Fiber Sclereid Pear (Pyrus) Permanent tissue: Complex permanent tissue: Simple Complex Vessel member Epidermis Xylem XYLEM Cork Tracheid • Conduct water Phloem and dissolved Parenchyma Xylem fiber minerals • Support Collenchyma Xylem parenchyma Sclerenchyma Xylem: Complex permanent tissue: Vessel Tracheid PHLOEM • conduct food and other organic substances Phloem Phloem Sieve tube Companion fiber parenchyma member cell • dead at maturity • end wall - imperforation • end wall - perforation • gymnosperm • angiosperm Phloem: Monocot & Dicot: Sieve tube Companion cell •sieve plate Monocot & Dicot: Stem monocot: Monocot stem, c.s. Dicot stem, c.s. X - section Stem monocot: Stem dicot: Vascular tissue Epidermis Cortex Vascular bundle Pith X - section X - section Stem dicot: Stem dicot: Epidermis Cortex Pith Phloem Xylem Stem dicot: Lab. session: Vascular tissue Permanent Slide Meristem tissue Permanent tissue Monocot & Dicot • L-S), Allium sp. • Epidermis • Compare root • Periderm • X-S, corn stem •X-S, Coleus • Collenchyma •X-S, Coleus sp. sp. stem stem • Sclerenchyma • Vascular bundle • Parenchyma • Fiber • Chlorenchyma X - section Free hand sectioning: Lab. results: Fiber ของวานฝกดาบ Wet mount •X-S, หญาละออง •X-S, วานฝกดาบ •X-S, ฝรั่ง • กะลามะพราว • Safranin dye Safranin stained Aniline sulfate stained • Aniline sulfate Lab. results: Lab. results: X-S หญาละออง Sclerenchyma ของฝรั่ง Vascular tissue, safranin stained Sclereid, safranin stained.