DOCSLIB.ORG

Synthetic Components Network (SCN)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Synthetic Components Network (SCN) SSCCNN LLooggoo The first annual conference of the SSyynntthheettiicc CCoommppoonneennttss NNeettwwoorrkk SSyynntthheettiicc BBiioollooggyy ffrroomm tthhee bboottttoomm uupp 23 – 24 September 2009 St Anne’s College Oxford SCN Annual Conference, 23rd – 24th September 2009 Welcome Welcome message Dear all, Welcome to St Anne’s College, and the first Annual Conference of the Synthetic Components Network (SCN). First of all thank you to everyone who has contributed to the exciting conference programme, which represents the breadth and depth of research and expertise across the Network. We hope that the programme, together with the poster sessions, will provide a real insight into some of the work already going on in the Network, and highlight some of the areas that are ripe for collaboration. Indeed, our overall objective is to foster communication and collaborations across the Network, but, of course, whether this happens is ultimately up to you. We are also very pleased to welcome the PIs of three other BBSRC/EPSRC-funded Networks in Synthetic Biology. They will be taking part in the panel Q&A session on Wednesday evening. The aim here is not to define what synthetic biology is, but to explore what people understand by it more broadly. We hope you will use this session to find out about how the other Networks view Synthetic Biology, learn something of the projects that are underway in them, and to explore opportunities for wider collaborations. Because new interactions and exploring potential collaborations are key, we have left plenty of time for networking and discussion over lunches, dinner, poster sessions, and in the bar! Finally, your feedback on how this meeting goes, and how it might be improved in future will be very welcome. Please give this to us, to Kathleen Sedgley directly, or use the tear-out form at the end of this programme. We hope you enjoy this first Annual Conference and the scientific and social interactions that it fosters. Best wishes, Dek Woolfson and Jonathan Rossiter 2 SCN Annual Conference, 23rd – 24th September 2009 Contents Table of Contents Welcome from Dek 2 Programme 4 Session 1: Components and self-assembly 6 Session 2: Encapsulation 11 Session 3: ELSI 15 Session 4: Responsive materials and movement 16 Session 5: Public engagement 19 Session 6: Towards systems 20 Panel Q&A session: What is Synthetic Biology and who cares? 25 Poster session PIs 29 PhDs and PDRAs 34 Delegate list 44 Thank you 46 Researcher exchange form 47 Feedback form 49 Maps 51 3 SCN Annual Conference, 23rd – 24th September 2009 Programme Programme ------------------------------------------------------------------------------------------------------------------ WEDNESDAY 23RD ------------------------------------------------------------------------------------------------------------------ 09-30 – 10-20 Arrivals and coffee 10-20 – 10-30 Welcome Dek Woolfson, Bristol Session 1: Components and self-assembly Chair: Louise Serpell, Sussex 10-30 – 11-00 William Taylor, NIMR Stick models of proteins 11-00 – 11-30 Andrew Turberfield, Oxford DNA nanostructures and molecular machinery 11-30 – 11-50 Karen Marshall, Sussex Structures of amyloid-like fibres and crystals formed from a self-assembling peptide 11-50 – 12-10 James Arpino, Cardiff Engineering allosteric control over GFP fluorescence 12-10 – 12-30 Craig Armstrong, Bristol Peptide components for Synthetic Biology 12-30 – 14-00 Lunch Session 2: Encapsulation Chair: Andrew Turberfield, Oxford 14-00 – 14-30 Steve Evans, Leeds Encapsulation: lipid membranes 14-30 – 15-00 Hagan Bayley, Oxford Engineered protein pores as components of soft micromachines 15-00 – 15-20 Nikolaos Daskalakis, Leeds Generating proton-motive force in probe-loaded vesicles 15-20 – 15-40 Jon Howse, Sheffield Vesicles by design 15-40 – 17-00 Posters Tea Session 3: ELSI ELSI research in SynBio: What’s being done? What should be done? 17-00 – 18-00 Lead: Ainsley Newson, Bristol 18-00 – 18-30 Drinks 18-30 – 20-00 Dinner ~ Wednesday’s programme continues over leaf ~ 4 SCN Annual Conference, 23rd – 24th September 2009 Programme Panel What is Synthetic Biology, and who cares? Discussion Short talks form PIs of Networks in Synthetic Biology followed by a panel Q&A session Chair: Kathy Sykes, Bristol 20-00 – 21-30 Rob Edwards, Durham SPPI-NET: a network for synthetic plant products for industry Alistair Elfick, Edinburgh SynBio Standards: Standards for the design and engineering of modular biological devices John Ward, UCL Synbion: the UCP Network in Synthetic Biology Dek Woolfson, Bristol Synthetic Components Network 21-30 - late Bar ------------------------------------------------------------------------------------------------------------------ THURSDAY 24TH ------------------------------------------------------------------------------------------------------------------ Session 4: Responsive materials and movement Chair: Mark Dillingham, Bristol 09-00 – 09-30 Neil Cameron, Durham Responsive materials from sugars and peptides 09-30 – 10-00 Richard Berry, Oxford Protein motors 10-00 – 10-20 Joe Yeeles, Bristol Activation of a helicase motor protein upon encounter with a specific sequence in the DNA track 10-20 – 11-30 Discussion session Coffee Session 5: Public engagement Public engagement with Synthetic Biology 11-30 – 12-30 Lead: Philippa Bayley 12-30 – 14-00 Lunch and discussion (Management Committee Meeting) Session 6: Towards systems Chair: Beth Bromley, Bristol & Durham 14-00 – 14-30 Caroline Colijn, Bristol Mathematical modelling in Synthetic Biology 14-30 – 15-00 Nigel Savery, Bristol Gene regulation by natural and synthetic components 15-00 – 15-20 Mike Sternberg, Imperial Tools for protein modelling 15-20 – 15-40 Paul Gardner, Oxford Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria 15-40 – 16-00 Eric Tippmann, Cardiff Application of physical organic chemistry to an expanded genetic code 16-00 – 16-10 Summary and departures 5 SCN Annual Conference, 23rd – 24th September 2009 Session 1: Components and self-assembly Taylor, William Institution: National Institute for Medical Research Oral presentation Title: Stick models of proteins Key words: Protein structure / topology / fold space Abstract The specification of a protein fold is typically viewed as poorly defined, being sensitive to secondary structure elements that may be based on marginal or unconserved hydrogen bonds. We use of a secondary structure lattice based on regular layers of secondary structure to overcome this problem. or decoy models, the structure is built directly from the lattice and so their fold is predefined. For known structures, the ideal lattice must be matched to the structure and although this step might appear susceptible to marginal effects, we do not rely on a single best fit but retain all good fits to different lattice cores. This does not provide a unique definition of a protein fold but a match to any of the variations counts as a hit, giving a ``fail-safe'' position towards matching known folds. These predefined fold definitions can be encoded as simple string of text (called a topology string), and there is no ambiguity in whether a pair of folds are the same as they will either have identical strings or strings that contain mismatches. This approach avoids the problems encountered using RMSD-based measures associated with length differences and statistical significance. We used the more familiar RMSD measures as an extensive cross-check on our topological measure and it was clear that it could not distinguish many distinct topological changes against the background 'noise' associated with secondary structure shifts and loop variation. This level of protein structure analysis has been used in the prediction of protein structure as well as the classification of structure. Most recently these two studies have been combined in an analysis of the folds generated during structure prediction. Of the many thousands of distinct folds constructed, only one-in-ten were found to exist among the known structure. The remainder have been likened to cosmological dark matter. Taylor, W.R ., Chelliah, V., Hollup, S.H., MacDonald, J.T., Jonassen, I. (2009) “Probing the 'dark matter' of protein fold-space”. Structure 17:1244-1252 6 SCN Annual Conference, 23rd – 24th September 2009 Session 1: Components and self-assembly Turberfield, Andrew Institution: University of Oxford Oral presentation Title:0B DNA nanostructures and molecular machinery Key words: Nanostructure / self-assembly / molecular motor / Brownian ratchet Abstract DNA is a wonderful material for nanoscale construction: it is a structural material whose self-assembly can be programmed by making use of its information-carrying capability, and its hybridization or hydrolysis can be used as to provide energy for molecular devices. I shall describe our recent work on self-assembled molecular structures and on molecular machinery, including the free-running bipedal molecular motor shown above, which is a chemically fuelled Brownian ratchet inspired by kinesin. [1] Yurke, B., Turberfield, A.J., Mills, A.P., Simmel, F.C. & Neumann, J.L. A DNA- fuelled molecular machine made of DNA. Nature 406, 605-608. (2000) [2] Turberfield, A.J. et al. DNA fuel for free-running nanomachines. Phys. Rev. Lett. 90, 4 (2003) [3] Goodman, R.P. et al. Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication. Science 310, 1661-1665 (2005) [4] Erben, C.M., Goodman, R.P. & Turberfield, A.J. Single-Molecule Protein Encapsulation in a Rigid DNA
Load more

Recommended publications
  • 35566 Annreport06 Txt 15-18
    02 pp15-18 students.qxp 24/11/06 12:54 Page 1 STUDENTS The student experience has always been characterised by transition, change and development – that’s what higher education is for. But as the landscape of education itself undergoes radical change, Bristol’s enterprising students continue to excel in their chosen fields and branch out into extra-curricular activities with energy and imagination. Postgrads rally to Mongolia Two Bristol postgraduates completed one of the most extreme car challenges in the world – the 8,000-mile Mongol Rally – in an old Volkswagen Polo. Dan Bailey (Department of Mathematics) and George Chapman (Department of Physics) covered a quarter of the Earth’s surface in a car with a one-litre Right: Key members of the Bristol/Havana engine, driving on roads ranging from bad to team.Top, l-r: Robert almost non-existent, with no support vehicles Cottrell, Hayley Sharp and obstacles including two deserts and five Jose Ernesto Gonzalez Hugo Baker. Bottom, mountain ranges. l-r: Ian Baggs, Alejandro Perez The Mongol Rally raises funds for two Malagon. Inset: Machinery inside a charities: ‘Send a Cow’, which provides poor pump house. farmers in Africa with livestock, training and advice; and ‘Save the Children in Mongolia’. Engineers without Borders Competitors’ cars must have an engine no bigger than 1,000cc. After completing the Four Bristol students flew out to Havana in rally in 27 days, Dan and George arrived in July in a bid to improve the Cuban capital’s Ulaan Bataar, where they donated their car to water supplies. The Engineers Without Save the Children in Mongolia.
    [Show full text]
  • INAUGURAL SCN MEETING 24 – 25 March 2009
    INAUGURAL SCN MEETING 24 – 25 March 2009 Coombe Lodge, Bristol Dear Network Member, First of all, welcome to Coombe Lodge, and welcome to the Inaugural Meeting of the Synthetic Components Network (SCN). The SCN is one of seven Reseach Council-funded Networks in Synthetic Biology. Synthetic biology is one of the BBSRC’s ten new research priorities and is signposted at EPSRC. The Research Council’s aims in setting up the Networks include: to engender a culture of taking a synthetic-biology approach in biological sciences and engineering in the UK; to grow a UK community in synthetic biology; and to help define* what is new and emerging research area, with many interested parties from a broad collection of disciplines. *Though, like many, we feel that keeping this definition as loose and broad as possible may be the best and healthiest option. As stated in our original proposal for the SCN, in broad terms its scientific aim is ‘…to address the challenge of creating new biological and biomimetic systems by combining de novo designed molecular components, pared-down biological moieties and engineering-design principles to build self-organising, functional biomolecular systems’. Now that we are gathered together for the first time, some of the things that we should address are: is this aim appropriate and tractable, and can we better-define what we would like to achieve through the Network? In considering this, however, in some respects this a discussion exercise because we do not have funds for research as such, though we hope sincerely that major grant applications will emerge from it.
    [Show full text]
  • BBSRC Support for Industrial Biotechnology and Bioenergy
    BBSRC support for Industrial Biotechnology and Bioenergy Dr Colin Miles What is UK Research and Innovation (UKRI) ? • UKRI is the new funding organisation for research and innovation in the UK. • UK Research and Innovation launched in April 2018. • UKRI comprises seven UK research councils, Innovate UK and a new organisation, Research England, working closely with its partners in the devolved administrations UKRI Strategic Prospectus : Objectives UKRI objectives: − Pushing the frontiers of human knowledge − Delivering economic impact and prosperity − Creating social and cultural impact − Providing the best foundation and environment for research and innovation − Delivering and being accountable as an outstanding organisation What does BBSRC do ? Invests in world- Invests in class bioscience bioscience training research in UK & skills for the next Universities & generation of Institutes bioscientists Drives the widest possible social & Promotes public economic impact dialogue on from our bioscience bioscience BBSRC Forward Look for UK Bioscience Advancing the frontiers of bioscience discovery Understanding Transformative the rules of life technologies Tackling strategic challenges Bioscience for Bioscience for Bioscience for renewable an integrated sustainable resources and understanding agriculture and food clean growth of health Building strong foundations Collaboration, People Infrastructure partnerships and talent and KE BBSRC Vision and Strategy: IBBE (2013-16) VISION: UK bioscience research delivering new products and processes for
    [Show full text]
  • The 7Th Alpbach Workshop On: COILED-COIL, FIBROUS
    The 7The 7th Ath Alpbach Workshop on: lpbach Workshop on: COILEDCOILED--COILCOIL, , FIBROUS FIBROUS & & REPEAT REPEAT COILED-COIL, FIBROUS & REPEAT PROTEINSPROTEINS At The Romantikhotel BöglerhofAt The Romantikhotel Böglerhof, Alpbach, Austria, Alpbach, Austria, Alpbach, Austria Sunday Sunday 3rd 3rd September– Friday September– Friday 8th 8th September September 20172017 7th Alpbach Workshop on: Coiled-coil, fibrous and repeat proteins SUNDAY SEPTEMBER 3 Arrivals, Reception and Dinner MONDAY SEPTEMBER 4 09:00 – 10:40 New developments in coiled coils (Andrei Lupas) Andrei Lupas (MPI Tübingen) - Coiled coils - between structure and unstructure Partho Ghosh (UC San Diego) - Functional essential instability in the M protein coiled coil Marcus Jahnel (MPI Dresden) - Coiled-coils as molecular motors: multistable polymer engines Jeni Lauer (MPI Dresden) - Structural Dynamics of the Rab5- Modulated Coiled-Coil Protein EEA1 Revealed by Hydrogen- Deuterium Exchange Mass Spectrometry 10:40 – 11:10 Tea break 11:10 – 12:30 Of α-fibers and β-fibers (Andrei Lupas) Birte Hernandez Alvarez (MPI Tübingen) - α/β-coiled coils Antoine Schramm (Marseille) - Characterization of measles virus phosphoprotein: A coiled-coil domain containing conserved motifs that are crucial for its function Michelle Peckham (Leeds) - Stable single α helices that do not form coiled coils - what makes them stable? 12:30 - Lunch Afternoon free 17:00 – 17:50 Flash Presentations for Posters 17:50 – 18:30 Posters 18:30 – 20:00 Dinner 20:00 – 21:40 Coiled coils at the membrane (Alexander
    [Show full text]
  • Appendices Emerging Biotechnologies
    Appendices Emerging biotechnologies Appendix 1: Method of working Background The Nuffield Council on Bioethics established the Working Party on ‗Emerging biotechnologies‘ in January 2011. The Working Party met eleven times over a period of 18 months. In order to inform its deliberations, it held an open consultation and a series of 'fact-finding‘ meetings with external stakeholders and invited experts. It also commissioned two reports on topics relevant to the work of the project and received comments on a draft of the Report from 12 external reviewers. Further details of each of these aspects of the Working Party‘s work are given below and in Appendix 2. The Working Party would like to express its gratitude to all those involved for the invaluable contribution they made to the development of the final Report. Consultation document The Working Party launched a consultation in April 2011, which ran until July 2011. 84 responses were received, of which 48 were submitted by individuals and 36 on behalf of organisations. Those responding to the consultation included students, academics, faith groups and professional organisations. A full list of those responding is set out in Appendix 2. A summary of the responses is available on the Council‘s website. Copies of individual responses will also be made available on the website in those instances where the Council has permission from respondents to do so. Fact-finding As part of its work, the Working Party held a series of 'fact-finding‘ meetings, the details of which can be found below. (Details
    [Show full text]
  • Electronic Control of Protein Interactions Mark Anthony Sellick
    1 Electronic Control of Protein Interactions Mark Anthony Sellick Submitted in accordance with the requirements for the degree of Doctor of Philosophy The University of Leeds Centre for Doctoral Training in Molecular-Scale Engineering School of Electronic and Electrical Engineering September 2016 2 3 The candidate confirms that the work submitted is his own and that appropriate credit has been given where reference has been made to the work of others. This copy has been supplied on the understanding that it is copyright material and that no quotation from the thesis may be published without proper acknowledgement. © 2016 The University of Leeds and Mark Anthony Sellick The right of Mark Anthony Sellick to be identified as Author of this work has been asserted by Mark Anthony Sellick in accordance with the Copyright, Designs and Patents Act 1988. 4 5 Acknowledgements I would first like to acknowledge my supervisor Professor Christoph Wälti for his invaluable guidance and support during my studies. Doctor Steven Johnson and Doctor Simon White, the other members of the coiled coil team, have also been integral to this project and I thank them for their assistance and friendship. I would also like to thank Doctor Franziska Thomas and Professor Dek Woolfson for their insights and assistance designing and synthesising the peptides used in this work. I must also thank all the members of the Bioelectronics Group, past and present, for their expertise and friendship over the years. Thanks also to the members of the various biology and chemistry groups in Leeds and Bristol for helping the confused physicist who could occasionally be found in their labs asking odd questions and trying not to break anything.
    [Show full text]
  • Bristol Biodesign Institute
    WELCOME Bristol BioDesign Institute Biomolecules to biosystems from understanding to design The Bristol BioDesign Institute (BBI) is one of the University of Bristol’s Specialist Research Institutes. The BBI brings together BrisSynBio, a UK Synthetic Biology Research Centre, the SynBio Centre for Doctoral Training, our Innovation Programme and Public Engagement activities. With wide-ranging applications from health to food security, BBI combines pioneering synthetic biology approaches with understanding biomolecular systems to deliver the rational design and engineering of biological systems for useful purposes. This is delivered through multidisciplinary research which brings together postgraduate and postdoctoral researchers, academics, policy makers and industry, whilst also engaging the public with emerging solutions to global challenges. The BBI places the University of Bristol among the forerunners of UK and international synthetic biology and biodesign research, teaching and innovation. Director: Professor Dek Woolfson Co-Directors: Professor Imre Berger Professor Claire Grierson Professor Mario di Bernardo Conference organising committee: Imre Berger, BrisSynBio Director and Bristol BioDesign Institute Co-Director Graham Day, PhD Student, School of Cellular and Molecular Medicine Bethany Hickton, PhD student, School of Cellular and Molecular Medicine Kathleen Sedgley, Bristol BioDesign Institute Manager Mark Winfield, Post-doctoral Research Assistant, School of Biological Sciences Marie Woods, Bristol BioDesign Administrator Dek
    [Show full text]
  • 005 School of Biochemistry & Cell Biology Newsletter
    VOLUME 1 ISSUE 5 SCHOOL OF BIOCHEMISTRY AND CELL BIOLOGY NEWSLETTERMARCH 2014 have the potential to offer major opportunities as well as Message from Head of School presenting enormous challenges. In this issue th February 28 was a red-letter day for this School as a Not to be outdone, a number of other PhD students Relay for Life total of 35 students graduated with MSc’s and 4 with have since successfully completed their PhD viva voce Biochemists in a Beautiful World PhD’s at the SEFS conferring ceremony held in Áras examinations and will be graduating next summer. Spring Conferrings na Mac Léinn. This is a testament to the hard work of Upcoming Events the students themselves, and also, their lab mates and I hope you find this Newsletter interesting and that it supervisors who helped them through their project helps keep you in touch with the School of Biochemistry Hot Off the Press work. and Cell Biology. In particular, it is noteworthy that of the 12 students who graduated at this conferring represented the first ever cohort of the MSc in Molecular and Cell Biology with Professor David Sheehan Bioinnovation organised by Dr Maryanne O’Donovan and Dr Kellie Dean. This MSc is a new taught programme that we plan to develop further into the future. The conferring was addressed by Rear Admiral Mark Mellet, the highest-ranking officer in the history of the Naval Service, who delivered a thoughtful and stirring speech around the idea of “constructive disruption”. He proposed that we are living through a time when novel SCHOOL(some as-yet unthought of) “disruptive” technologies PAGE 2 With students and staff from the School of Biochemistry and Cell Biology, Team Mad Scientists participated in Donations to sponsor the second Relay for Life UCC, on Friday, 28th February, Team Mad Scientists in 2014, in support of the Irish Cancer Society.
    [Show full text]
  • Contributors to the Emerging Investigators Issue
    View Article Online / Journal Homepage / Table of Contents for this issue Analyst Dynamic Article LinksC< Cite this: Analyst, 2011, 136, 3406 www.rsc.org/analyst PROFILE Contributors to the Emerging Investigators Issue DOI: 10.1039/c1an90056k University of Illinois at Urbana-Cham- paign and is affiliated with the University’s Institute for Genomic Biology and Micro and Nanotechnology Laboratory. Ryan received his BS in Chemistry from Eastern Illinois University in 1999 and his PhD in Chemistry from Northwestern University in 2004, after which he was a joint post- doctoral fellow at the California Institute of Technology and Institute for Systems Biology. His research interests lie in the development of new multiparameter bio- logical analysis technologies for applica- Jeffrey Anker is an Assistant Professor of tions in informative disease diagnostics, Christa Brosseau is from Halifax, Nova Chemistry at Clemson with a BS in applied homeland security, and fundamental bio- Scotia. She attended Dalhousie University physics (Yale University, 1998), and PhD logical studies. (BSc) and Acadia University (MSc) before from the University of Michigan (2005). As goingontocompleteaPhDininterfacial a graduate student with Raoul Kopelman he electrochemistry and vibrational spectros- developed magnetically modulated optical copy under the supervision of Jacek Lip- nanoprobes (MagMOONs) for chemical kowski at the University of Guelph in and mechanical measurements. For this Ontario, Canada. She was a postdoctoral work, he received a Grand Prize at the 2003 fellow in the laboratory of Richard Van Published on 28 July 2011. Downloaded 30/10/2014 08:00:28. National Inventor’s Hall of Fame Collegiate Duyne, and worked towards the application Inventor’s Competition.
    [Show full text]
  • EPSRC & BBSRC Synthetic Biology Centre for Doctoral Training
    EPSRC & BBSRC Synthetic Biology Centre for Doctoral Training Student Handbook 2015 1 Contents Introduction 3 Programme Background 4 Key Objectives Programme Summary 7 Module Outlines 8 Core Modules; Career Development Skills; DTC Teaching Experience, Graduate Academic Programme Projects 18 The Supervisory Relationship Monitoring and Assessment 20 Student Space, Weblearn, Module Assessment; Module Assessment Submission; Module Questionnaires; Project Assessment; Project Assessment Submission; Academic Integrity; Project Questionnaire; Transition to DPhil; DPhil Assessment; Oxford Digital Theses; Alumni DTC Fourth Year Symposium Student Representatives Internships Resources 34 Libraries; DTC Library; Computing Facilities; Seminar Programmes; Telephone/Email/Mail Staff 39 DTC Staff; SynBioCDT Staff, Affiliated Staff Rex Richards Building 53 Parking; Access and Security; Fire Alarms; Office Etiquette; First Aid University of Oxford, Bristol and Warwick 55 University Card; Careers Service; Medical Care; Student Associations; Student Counselling Service; Sports and Physical Recreation; Support for Student Parents Administrative Matters 61 Programme Management; Position within the University; Timekeeping; Attendance; Absence/Illness; Academic Terms; Finance; Proof of Study, Equality and Diversity Unit; Interruption of Studies; University Health and Safety; Data Protection City of Oxford, Bristol and Warwick 69 Travelling to Information; Maps Appendix 77 MPLS Research Supervision, A Brief Guide 2 Introduction Welcome to the EPSRC & BBSRC Centre for Doctoral Training in Synthetic Biology (SynBioCDT), which is a joint venture between the Universities of Oxford, Bristol and Warwick. SynBioCDT is a 4-year Doctorate Programme in the interdisciplinary field of Synthetic Biology, which has been identified as an emerging discipline with the potential to create new industries and economies in the recent UK Synthetic Biology Roadmap report (http://www.rcuk.ac.uk/publications/reports/syntheticbiologyroadmap/).
    [Show full text]
  • Baker, EG, Bartlett, GJ, Porter Goff, KL, & Woolfson, DN (2017
    Baker, E. G. , Bartlett, G. J., Porter Goff, K. L., & Woolfson, D. N. (2017). Miniprotein design: past, present, and prospects. Accounts of Chemical Research, 50(9), 2085-2092. https://doi.org/10.1021/acs.accounts.7b00186 Peer reviewed version License (if available): Unspecified Link to published version (if available): 10.1021/acs.accounts.7b00186 Link to publication record in Explore Bristol Research PDF-document This is the author accepted manuscript (AAM). The final published version (version of record) is available online via at ACS Publications http://pubs.acs.org/doi/abs/10.1021/acs.accounts.7b00186. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ Miniprotein Design: Past, Present and Prospects Emily G. Baker,1† Gail J. Bartlett,1† Kathryn L. Porter Goff,1† and Derek N. Woolfson1,2,3†* 1School of Chemistry, University of Bristol, Cantock’s Close, Bristol, BS8 1TS, UK 2School of Biochemistry, University of Bristol, Biomedical Sciences Building, University Walk, Bristol, BS8 1TD, UK 3BrisSynBio and the Bristol BioDesign Institute, University of Bristol, Life Sciences Building, Tyndall Avenue, Bristol, BS8 1TQ, UK *Corresponding author: DNW ([email protected]) †All authors contributed equally to this review. CONSPECTUS: The design and study of miniproteins – that is, polypeptide chains < 40 amino acids in length that adopt defined and stable 3D structures – is resurgent.
    [Show full text]
  • Brissynbio: a BBSRC/EPSRC-Funded Synthetic Biology Research Centre
    Sedgley, K. R., Race, P. R., & Woolfson, D. N. (2016). BrisSynBio: a BBSRC/EPSRC-funded Synthetic Biology Research Centre. Biochemical Society Transactions, 44(3), 389-391. DOI: 10.1042/BST20160004 Publisher's PDF, also known as Version of record License (if available): CC BY Link to published version (if available): 10.1042/BST20160004 Link to publication record in Explore Bristol Research PDF-document This is the final published version of the article (version of record). It first appeared online via Portland Press at http://www.biochemsoctrans.org/content/44/3/689. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms Synthetic Biology UK 2015 689 BrisSynBio: a BBSRC/EPSRC-funded Synthetic Biology Research Centre Kathleen R. Sedgley*1, Paul R. Race*† and Derek N. Woolfson*†‡ *BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K. †School of Biochemistry, University of Bristol, Bristol BS8 1TD, Avon, U.K. ‡School of Chemistry, University of Bristol, Bristol BS8 1TS, Avon, U.K. Abstract BrisSynBio is the Bristol-based Biotechnology and Biological Sciences Research Council (BBSRC)/Engineering and Physical Sciences Research Council (EPSRC)-funded Synthetic Biology Research Centre. It is one of six such Centres in the U.K. BrisSynBio’s emphasis is on rational and predictive bimolecular modelling, design and engineering in the context of synthetic biology.
    [Show full text]