AIBN ANNUAL REPORT 2010 33 STUDENT EXPERIENCE 34 RHD report 36 Student profi le: Mirjana Dimitrijev Dwyer 37 Graduate profi le: Anthony Musumeci 38 2010/11 Summer internship 39 Occupational trainees 40 Research Higher Degree students 1 Vice-Chancellor’s message 41 SENIOR RESEARCHERS 2 Director’s message 42 Professor Peter Gray 4 Unique identity recognised in 43 Professor Kirill Alexandrov milestone review 44 Dr Bronwyn Battersby 6 AIBN benefi ts the economy 45 Associate Professor Idriss Blakey 7 RESEARCH ACHIEVEMENTS 46 Professor Julie Campbell 8 Integrated approach to improving 47 Professor Justin Cooper-White human health 48 Dr Annette Dexter 10 Latest in vaccine technology 49 Dr Krassen Dimitrov 12 Biologics research grows at AIBN 50 Professor John Drennan 14 Metabolic engineering through systems 51 Associate Professor Aijun Du and synthetic biology 52 Professor Peter Halley 16 Nano developments for diagnostic 53 Professor Mark Kendall advances 54 Professor Max Lu 18 Collaboration advances energy research 55 Associate Professor Stephen Mahler 20 Novel polymers and nanostructures for 56 Associate Professor Darren Martin new generation of products 57 Professor Anton Middelberg 22 Our vision 58 Professor Michael Monteiro 59 Professor Lars Nielsen 23 ENGAGEMENT 60 Dr Steven Reid 24 New Dow MOU 61 Professor Sean Smith 25 Biojet 62 Professor Matt Trau 26 Fabrication facilities open doors to 63 Associate Professor Christine Wells industry 64 Professor Andrew Whittaker 27 Continuing professional development 65 Associate Professor Ernst Wolvetang 28 Engaging through affi liates program 66 Professor Chengzhong (Michael) Yu 29 2010 HIGHLIGHTS 67 GRANTS 30 Fellowships 73 PUBLICATIONS 31 ARC achievement 32 Awards 79 AIBN SEMINARS 2010 vice-chAncellor’s messAge

AIBN offers proof that high-quality research generates excellent results for society, industry and the economy.

In 2010 AIBN, in its eighth year, continued A third evaluation of AIBN, a seven-year to build on its record of conducting excellent review sanctioned by the UQ Senate and fundamental research and harnessing it to led by a global panel, including experts benefi t health, the environment and industry. from Harvard and University College London, commended the Queensland By early 2011 the national litmus test of Government, investors and UQ for “foresight universities’ research, the Excellence in in establishing AIBN with the remit to link Research for Australia (ERA) assessment, translation of research to commercial use”. had confi rmed AIBN as a national leader and a performer well above world standards The results from ERA, the Allen Group and in its fi elds. The University of Queensland’s the seven-year review can all be read as pinnacle ERA ratings in environmental vindicating the enabling investments by the biotechnology, industrial biotechnology and Queensland Government and The Atlantic nanotechnology owed signifi cant credit Philanthropies, as well as subsequent to AIBN. program and equipment funding from the Australian and Queensland governments. What ERA did not show – but this annual The results also endorse the commercial report does – is the value added to wisdom of Australian businesses and global high-quality discovery as it progressively corporations that network with AIBN. transmutes into products and services that industry and communities demand. With its focus on areas including human health, information and communications An assessment in 2010 by the Allen technology, energy, manufacturing and the Consulting Group clearly articulates environment, AIBN will increase its output the institute’s economic impact. Using of research that is high impact and very Queensland Government-approved relevant to communities and industry in methodology, the group found every Queensland, Australia and globally. additional dollar invested in AIBN during 2003-2009 would increase Queensland’s I commend Peter Gray and his economic output by $4.50 to $6.30, while colleagues for their sustained the national multiplier effect would be in the excellence. range of $2 to $2.90.

The Allen Group report forecast that Professor Paul Greenfi eld AO AIBN would boost state and Australia- Vice-Chancellor wide consumption and investment, The University of Queensland and remarked on the “strong practical application opportunities in existing major Queensland industries”. It concluded that further state government investments would ensure the Queensland Government’s ongoing leadership in bioengineering and nanotechnology.

1 director’s messAge

2 The year 2010 has turned out to A measure of the impact of AIBN research be a milestone one for AIBN in is demonstrated by Queensland Premier Anna Bligh highlighting two AIBN-linked many ways. projects at a press conference at the prestigious US BIO conference. One was During the year the institute was involved the announcement, with Boeing’s chief in three important reviews. We were the technology offi cer John Tracy and then fi rst UQ research institute to be reviewed by Amyris Biotechnology chief fi nancial offi cer UQ’s Academic Board, a formal process the Dr Kinkead Reiling, of government support university conducts every seven years on for the Queensland Sustainable Aviation all academic and research units; the Allen Fuel Initiative. This research consortium is Consulting Group conducted a review of led by Professor Lars Nielsen. AIBN’s economic contribution to Queensland The second, in association with DSM and Australia; and AIBN’s foundation group Biologics president Karen King, was leaders’ performance during the initial fi ve that DSM was establishing in Brisbane years was reviewed as per the terms of their a mammalian cell good manufacturing appointment contracts. practice production facility, which In this annual report we have summarised would draw on AIBN’s existing biologics the main fi ndings of these important reviews. expertise. During the same trip, the Premier In addition, we have used this opportunity announced support for Professor Anton to briefl y summarise some of the main Middelberg’s collaboration with Tianjin developments that have taken place since University, in China, and Professor Andrew Whittaker’s collaborative research project on the institute began research activities in spinal cord regeneration involving Ottawa mid-2005, and then started to expand Hospital and Monash University. activities in a major way in 2006 with the move into the new AIBN building. Since the In a move certain to continue AIBN’s move to the new building there has been success in international collaboration, the a rapid expansion in high-quality research institute welcomed new Group Leader and, pleasingly, many new multidisciplinary Professor Chengzhong (Michael) Yu, from projects that have been instigated and Fudan University, China. Professor Yu has grown successfully out of opportunities received several awards, including an ARC presented by the co-location of the range Future Fellowship. of expertise and disciplines in AIBN. Our Shanghai World Expo was a fi tting place numbers have grown from 56 people in the for UQ Deputy Vice-Chancellor (Research) initial group leaders’ groups to a headcount and AIBN Group Leader Professor Max Lu of more than 400. to be recognised, during Queensland Week The growing successes of AIBN’s celebrations, with a Queensland Education researchers resulted in the institute’s income and Training International Queensland- exceeding $37 million in 2010, with the China Education and Training Award for majority of this raised from competitive Excellence. Within the awards, Professor Lu sources. It included funding from companies was named a joint winner in the Alumnus of for collaborative research programs, the Year (Research) category. support from the full spectrum of Australian In another 2010 achievement, the number research funding schemes, and support of research higher degree students enrolled from an expanding list of international through AIBN passed 100 and AIBN’s funding agencies and foundations. There Student Association further consolidated is a strong collaborative and international its position as a key factor in the institute’s fl avour to AIBN’s research. academic, cultural and social activities. We During 2010 we examined AIBN’s were delighted that Dr Ian Nisbet joined us international collaborations where there is during the year to run the Industrial Affi liates a contractual agreement in place between Program, bringing to AIBN his extensive the parties, such as a signed contract, experience in commercialising technology. a memorandum of understanding, a Finally I would like to thank Euan Murdoch, confi dential disclosure agreement or a who has so ably chaired our State material transfer agreement, and found Government review board, and our three 157 such collaborations. Vice-Chancellor senior colleagues and friends from UQ, Professor Paul Greenfi eld has already Deputy Vice-Chancellor (Academic) Professor commented that, in the recent Excellence Debbie Terry, Deputy Vice-Chancellor in Research for Australia exercise, AIBN’s (Research) Professor Max Lu and Vice- research fi elds were all judged to be Chancellor Professor Paul Greenfi eld for their performing at levels well above world advice and guidance throughout the year. standard. The 233 papers published in peer-reviewed journals during 2010 is further evidence of a sustained, high-quality Professor Peter Gray research output. AIBN Director

3 Unique identity recognised in milestone review

In its first seven years, AIBN has established a unique identity in the Australian research community, working relentlessly to become a dynamic, integrated, multidisciplinary, research institute merging skills, technologies and ideas in the core fields of bioengineering and nanotechnology. At its core, AIBN is focused on research excellence and industrial relevance.

A significant milestone for AIBN in 2010 external stakeholders; building and facilities Membership of the review was an independent review of its operations inspections; and written submissions. and achievements in the first seven years, committee: The review outcomes supported AIBN’s commissioned by UQ’s Academic Board Professor Mike Hoare (Chair) claim it had provided a critical mass of and reporting to UQ Senate. The review Department of Biochemical Engineering multidisciplinary researchers and a range of found AIBN had grown from nothing University College London cutting-edge facilities unique to Australia; more than a concept into a dynamic, produced world-class research; and Professor Tom Davis accomplished organisation with 19 group attracted world-class researchers. AIBN has Director, Centre for Advanced leaders; 370 researchers, students and leveraged external funds; provided a culture Macromolecular Design operational personnel; an annual budget of of commercialisation and entrepreneurial School of Chemical Engineering $34 million; and a 15,500sq m purpose-built, acumen; and contributed to educating University of New South Wales superbly equipped, research facility. and training a future generation of smart Dr Carrie Hillyard While those numbers are impressive on scientists and research leaders. Founder and Partner paper, it was AIBN’s advances in research CM Capital Investments Beyond those achievements, AIBN has and its growing international reputation Brisbane developed a research environment that that were considered among its greatest is accessible to industry and contributes Professor Regis Kelly achievements. The review commended significantly to the Queensland and Director, California Institute for Quantitative AIBN for developing a superb international Australian economies. Biosciences (QB3) reputation by recruiting top quality staff Professor Pam Silver and students; creating research facilities Data presented to the review committee Department of Systems Biology of international standard; nucleating hubs in June 2010 demonstrated AIBN’s Harvard Medical School of research excellence; partnering with impressive research achievements during the first seven years, including developing Professor Jennifer Stow leading international entities; and securing collaborative programs between disciplines Deputy Director (Research) significant research funding comparable and with industry, with more than 150 active Institute for Molecular Bioscience with leading US institutions. international research collaborations; $47.7 Professor Mike Hoare, from the Professor Alastair McEwan million in external research income, with Department of Biochemical Engineering Head, School of Chemistry and Molecular national and international competitive grant at University College London, chaired Biosciences schemes making up 85 percent of research the committee reviewing and assessing University of Queensland income; and 25,000 citations recorded AIBN’s performance, purpose, goals and Ms Helen Weir for group leaders. Those achievements priorities. The comprehensive review was Institute Manager continued during 2010, with external based on interviews with internal and Queensland Brain Institute research income reaching $68 million since inception of the institute.

4 Researchers have collaborations in 25 demonstrate discovery-to-translation countries and have forged links with leading pathways. The review committee made international organisations, such as Dow commendations and recommendations Chemical Company, Boeing, Amyris, Intel covering research, internationalisation, and Sematech. Four start-up companies commercialisation, governance, have been established on AIBN discoveries management and AIBN’s future growth. and innovations, with $1.755 million of The Queensland Government, investors equity raised for them. Data presented and the UQ Vice-Chancellor’s Executive to the review committee showed AIBN were commended for their foresight in researchers had 52 patent families under establishing AIBN with the remit to link management and the Industrial Affiliates translation of research to commercial use. Program, established in 2009, had 17 members. AIBN Director Professor Peter Gray was commended for his leadership in developing Among highlights in education and training the research direction and strong were attaining close to 100 research translational focus. AIBN’s management higher degree students; hosting seven was commended for creating international- international conferences and workshops; standard research facilities and making and establishing a Weekly Research resources available to the UQ community Seminar series and an Annual Institute that would not otherwise have been Research Symposium. The institute’s possible. strong support for teaching has seen AIBN researchers actively engaged in Further specific commendations covered teaching UQ undergraduate courses and the creation of research excellence hubs participating strongly in the University’s that enriched the scientific community in ResTeach Fellowship program. Recognition important areas, including drug delivery, for AIBN researchers’ leadership has seen molecular diagnostics, tissue engineering, three group leaders cited among Australia’s bioprocessing and functional nanomaterials, 100 most influential engineers. and AIBN’s partnerships with outside organisations in strategically important In completing the first review of a UQ areas. institute, the committee found AIBN’s approach to achieving excellence in research was embedded in its ability to

5 AIBN benefits the economy

AIBN aims to have a strong technology and industry focus as a key point of difference from other academic research institutions. In the seven years since AIBN was established, the institute has developed, through domestic and international industry-led collaborations, technologies with commercial applications across a range of industries.

Developing innovations that can be The report found AIBN’s total income during commercialised to benefit human health, the 2003-2009 was $174 million. It estimated environment, manufacturing, sustainable AIBN successfully attracted $61.5 million energy and information technology was a in additional investment to Queensland – key aim when AIBN was established. An that is, money that would not have been integrated, multidisciplinary framework invested in research or other activities in the ensured its research programs focused on state without the institute’s presence. the emerging disciplines of nanotechnology “The additional funds AIBN has attracted and bioengineering. to date are expected to enhance the The result has been a strong performance productivity of Queensland’s economy and across a range of areas, including therefore stimulate further investment and research outcomes, attracting investment, economic activity,” the report said. commercialisation, training and employment. “The additional funds attracted by AIBN That performance has provided a significant during 2003-2009 are also expected to contribution to the Queensland and make a significant ongoing contribution to Australian economies. In 2010, AIBN’s the Australian economy.” economic contribution was extensively analysed by the Allen Consulting Group, The report highlighted that AIBN had one of Australia’s leading economic and achieved a lot in the short period since public policy analysts. AIBN commissioned it was created. It went beyond monetary the group to review the institute’s economic figures to highlight the “strong practical contribution in its first seven years.The application opportunities” of the institute’s group found AIBN was highly successful research in existing major Queensland in attracting additional investment to industries, with the AIBN Industrial Affiliates Queensland, with the funds making a Program flagged as a useful mechanism significant contribution to the state’s to facilitate those opportunities and economy. Figures in the report showed applications. every $1 invested in AIBN during 2003- The report concluded further State 2009 increased Queensland’s economic Government investments in AIBN would output by $4.50 to $6.30. At a national ensure Queensland’s ongoing leadership level, the report suggested GDP increased in bioengineering and nanotechnology and between $2 and $2.90 for every additional ongoing economic contributions. $1 attracted by AIBN.

6 research achievements

Fast facts – AIBN achieved $68 million in external research income from formation until the end of 2010. – 85 percent of research income in AIBN’s first seven years was derived from national and international competitive grants. – 233 papers were published in refereed journals in 2010.

7 Integrated approach to improving human health

Personalised regenerative medicine is expected to transform health care globally and during the next five years alone is projected to be a $10 billion growth industry.

8 At the core of this transformation is the can be differentiated into all cell types of Professor Campbell has developed an convergence of the rapidly advancing fields the human body, providing the basis for autologous vascular graft in the peritoneal of transcriptomic, genomic, metabolomic future patient-specific stem cell therapies, cavity, a technique now being used in and proteomic technologies, which is novel disease models and drug screening overseas laboratories to grow autologous leading to unprecedented detailed insights platforms. heart valves. into the molecular processes that govern In a world first, Associate Professor “In simple terms, it means that, in the future, stem cell behaviour. A strongly held belief Wolvetang’s stem cell engineering group patients may be able to grow, within their at AIBN is that integrating cutting-edge has found a way to see how brain bodies, their own vascular graft to replace stem cell biology with nanotechnology development can go awry in people who go a diseased or damaged segment,” she said. will be the next critical step in translating on to show symptoms of Down syndrome. “This avoids either the sacrifice of a healthy this knowledge into clinical and industrial The group generated iPS cells from skin blood vessel to act as a graft, or implanting applications, such as drug screening. cells and stepwise differentiated them into foreign material as a prosthetic graft. It also Challenges facing the development of stem mature neurons. means there would be no rejection of the cell-based therapies and drug screening graft as it is derived from the patient’s own “This is a significant advance as it allows the applications include the need for defined cells.” deciphering of the fundamental molecular culture systems that allow scale up of stem processes that underlie altered brain In a world first, AIBN’s Professor Lars cell expansion and efficient, homogeneous development in Alzheimer’s disease and Nielsen and his research group have generation of defined lineage restricted Down syndrome,” Associate Professor developed a bioprocess to manufacture progenitor cells. There is also a need to Wolvetang said. neutrophils in therapeutic doses to protect deliver stem cells or stem cell-derived cells cancer patients at risk of invading bacteria “This will provide valuable insights into the to specific sites in the human body and and fungi. methods to address immune rejection. molecular basis of these conditions in the general population and constitutes a Professor Nielsen’s research group is With alleviating problems in human health drug screening platform for testing small working with the Australian Red Cross identified as a primary aim of AIBN research, molecules that can intervene in those and the Peter MacCallum Cancer Centre multiple integrated multidisciplinary conditions’ development.” to arrest the risk of neutropenia in cancer projects have been established at the patients undergoing chemotherapy. institute. One project pursues development Stem cell research is also a key element of of ‘smart’ surfaces and nanoparticles that Professor Justin Cooper-White’s research The infections associated with neutropaenia can control and direct stem cell fate in bulk group, which has made a significant impact cause about 200,000 cancer patients culture systems, such as bioreactors, and in the way biomaterials scientists design worldwide to be hospitalised each year and in microfluidic devices. Another involves smart surfaces for tissue repair. result in the death of one in 14 of those people. High-dose chemotherapy in cancer developing biodegradable scaffolds and Professor Cooper-White has developed new patients interferes with the body’s ability to functionalised nanoparticles for targeted insights into how synthetic biomaterials and produce neutrophils, a type of white blood delivery of stem cells. A third deals with microfluidic devices may be tailored to direct cell that is the body’s first line of defence efficient, cost-effective generation of patient- stem cell behaviour and tissue genesis, against invading bacteria and fungi. specific induced pluripotent cells (iPS cells). with particular application to regenerating The 2007 discovery that any cell of the meniscal tissue and, more recently, heart Professor Nielsen’s manufactured adult human body can be turned back into tissue. neutrophils, called TheraPhils, are not a stem cell therapy, but the product of iPS cells that can generate every cell type “Stem cells can turn into most or all tissues expanded haematopoietic stem and of the human body has revolutionised the in the body,” Professor Cooper-White said. progenitor cells (HPC) using either umbilical stem cell field. The iPS technology allows “The challenge is to make sure they turn into cord blood or peripheral blood donations as the production of stem, progenitor and the right tissue in the right location with an the cell source. The cells are fully functional, somatic cells that are patient specific, and appropriate timeframe for functional repair fully differentiated, non-genetically modified therefore unlikely to be immune rejected; of lost or damaged tissues. and development of disease-specific cell therapy products. The neutrophils “Our research aims to create synthetic personalised drug-screening platforms. are manufactured through a proprietary, microenvironments for optimised culture, patented process capable of achieving a Having core facilities for the culture and expansion and eventual implantation minimum 2,000 fold expansion of initial HPC generation of embryonic and iPS cells in the of stem cells. Our research outcomes population. That is a more than 10-fold AIBN building – combined with bioinformatic, have had a significant impact in terms of improvement in cell number expansion over nanofabrication, microfluidic, protein informing the way biomaterials scientists current methods and produces up to 30 production and metabolomic expertise – design surfaces and scaffolds for tissue transfusable doses. places the institute in a strong position to repair and informing the way biologists think TheraPhils have been tested for their ability lead the regenerative medicine revolution of about how cells currently grow in vitro. the 21st century. to respond to and kill microbes and behave “The outcomes of our research into similar to normal neutrophils. Like normal Associate Professor Ernst Wolvetang’s regenerative medicine will eventually neutrophils, they express low levels of research group has made significant improve the quality of life of people suffering antigens and should be safe to transfuse advances, creating a discovery platform from degenerative joint diseases and without tissue typing. for understanding the fundamental cardiovascular diseases.” processes that underpin cancer and neuro- The next step in Professor Nielsen’s Tissue repair techniques developed at AIBN degeneration. The research group has research is a small clinical study in patients by Professor Julie Campbell are starting generated iPS cells from people with ataxia with acute myeloblastic leukaemia to to be used in overseas laboratories, with telangiectasia, a rare neurodegenerative, demonstrate that the manufactured expectations that people will one day grow inherited disease that affects the brain and neutrophils are safe for use in humans different tissues in their own bodies to other parts of the body. The generated and behave like normal neutrophils when replace diseased or damaged body parts. cells are a renewable resource of cells that transfused.

9 Latest in vaccine technology

AIBN researchers have made significant inroads in the past seven years in identifying ways to potentially tackle H1N1 swine influenza and group A streptococcus, which can lead to rheumatic fever and rheumatic heart disease. They are working with global leaders in biochemistry to develop a vaccine platform. They have also developed a needle-free vaccine delivery device that has shown in preclinical trials to use 100 times less vaccine while still being protective.

A virus vaccine, but not a virus

Vaccines are a big business and save an However, Professor Middelberg and his After five years of scientific platform estimated eight million lives annually. When team quickly realised the yields of VLPs evolution, Professor Middelberg was Professor Anton Middelberg arrived at AIBN using those methods were far too low. “It delighted in 2010 to receive the Queensland from the University of Cambridge in 2003, was an interesting laboratory method, but a Smart Futures Premier’s Fellowship to he looked carefully at vaccine developments long way from being technologically useful.” further develop the VLP approach. “This and concluded that virus-like particles After careful research, AIBN PhD student $2.5 million grant will allow us to take (VLPs) were the future. At the time, UQ was Yap Pang Chuan and Protein Expression the VLP technology to the next level and winning acclaim for the work of Professor Facility manager Dr Linda Lua managed address specific diseases,” Professor Ian Frazer’s breakthrough VLP Gardasil, to achieve the highest expression of viral Middelberg said. “By grafting elements of a while Professor Middelberg had been protein in bacteria to date. The result was dangerous pathogen on our VLP, we will be researching virus assembly at Cambridge. published in a leading biotechnology journal able to direct the immune response towards He wondered what might be possible if he and patented jointly with Professor Garcea. a new or existing disease.” could create a “generic” VLP that could be With this early breakthrough, Professor The opportunities for better technology are tailored for any disease. Middelberg adopted a focused strategy huge. “There is currently no commercial “I looked first at Gardasil,” Professor of scientific platform development. In product or technology that can deliver Middelberg said. “It’s an absolutely brilliant 2008 and 2009 a series of AIBN-led tailored vaccines within weeks of a new VLP for cervical cancer, but not other successes emerged. New techniques for disease emerging. Take, for example, H1N1 diseases.” So he established a strategy the quantitative assessment of VLP size swine flu. Our VLP platform can potentially and team focused on a related VLP – and structure were developed, leading deliver vaccine in weeks, not months, polyomavirus. “VLPs are just fantastic. They to productive collaboration with the stopping a pandemic before it starts.” cause an enhanced immune response that US National Institute of Standards and Having recently secured a $1 million has both cell and antibody components. Technology. Bioreactor methods were collaboration with a leading group in China You can freeze dry them so there is no devised that led to gram-per-litre levels through the Smart Futures NIRAP program, need for a cold chain in the developing of VLPs from bacteria – the highest in and a contract from a US pharmaceutical world and they can be given by nasal puffer, the world – and a new analysis of the company, Professor Middelberg and his overcoming the need for a syringe.” As thermodynamic drivers of virus assembly team are convinced the future of vaccines is VLPs do not contain infectious DNA – they was published in the prestigious Journal of indeed VLPs – and that AIBN will continue are a shell of the virus but not the virus itself the Royal Society. In 2010, a cutting-edge to lead developments internationally. “This – they are inherently safe. mathematical model of VLP assembly approach to vaccines is ideally suited to was published by Professor Middelberg Armed with a $2 million Australian Research Queensland and will add huge value to the and his group and featured on the front Council Federation Fellowship, Professor state’s biotechnology infrastructure. A billion cover of the leading journal Biotechnology Middelberg’s first real breakthrough plus people in Asia want a healthy future and Bioengineering. A series of invitations came when the pioneer of VLP assembly, and deserve protection against new threats followed, including one to publish the Professor Robert Garcea, in Colorado, such as bird flu. This technology is ideally platform details in a special issue of the sent key materials and protocols to AIBN. suited to those markets and the product journal Vaccine. can be manufactured in Australia and shipped more easily than coal.”

10 Needle-free devices

Needle-stick injuries and cross- The Nanopatch overcomes inefficient needle Organisation estimates 50 percent of vaccines contamination are the major concern delivery, which places vaccine in the muscle, in Africa do not work properly because the in vaccination programs, prompting the where there are few immune cells. Instead, ‘cold chain’ has been broken.” development of a relatively new research the Nanopatch has thousands of small Professor Kendall’s group is part of the field in microneedle-based technology. projections designed to hit abundant immune International Needle-free Vaccination Since arriving at AIBN five years ago, cells in the skin. The projections, measuring Alliance, providing a vehicle for collaboration Professor Mark Kendall has established less than 1000 nanometres at the outer tips, with world leaders at the Seattle Biomedical himself as a leader in the emerging field. have led to seven patent applications. Research Institute, the Program for When Professor Kendall joined AIBN, there Preclinical trials in mice have shown Appropriate Technology in Health and the were no published articles anywhere in the unprecedented efficiencies, with a 100-fold World Health Organisation to investigate world about microneedle-based technology dose-sparing of influenza vaccine while the Nanopatch’s ability to vaccinate against for use as an alternative vaccination delivery achieving equivalent protection. three major diseases. technique. By 2010, there were more than “This is at least 10 times better than anybody “To help optimise our delivery devices, we are 40, with about half coming from Professor has achieved with any other delivery performing fundamental research into key Kendall’s research group. In that time, the method,” Professor Kendall said. “We are mechanical and biological properties of skin. first microneedle-based device has been world leading. Indeed, we extended the Mechanical measurements are made with a launched on the market. Nanopatch and have shown it is applicable range of tools, including a Nanoindentor. Our While Professor Kendall has not been the to particular disease test-cases of human understanding of skin biology is enhanced first to launch a product on the market, his papillomavirus, herpes simplex, West Nile with a range of tools, including a new in vivo work is at the cutting edge in both vaccine and Chikungunya viruses.” multi-photon microscope, one of very few in delivery and biomedical engineering. In just the world capable of interrogating – in real Initial data on dry-coating the vaccine to the five years, he has put together a research time – in vivo interactions within the skin Nanopatch tips has shown the technology group, developed the Nanopatch and induced after delivery. may not need refrigeration in vaccine transport entered a joint partnership research and and distribution. “This is hugely important,” “If we succeed, we are well on the path to development project with an international Professor Kendall said. “The World Health revolutionising the way we vaccinate.” vaccine company.

11 Biologics research grows at AIBN

Since the advent of recombinant Such therapeutics are sometimes referred to commercial production. The research DNA technology, a new class as biopharmaceuticals but, increasingly, the focuses on developing improved CHO cell term biologics is being used. New biologics lines and bioprocesses for transient and of protein-based human have gained rapid acceptance as human stable expression of biologics. therapeutics has been therapeutics, accounting for more than It is possible to introduce nucleic acids into 17 percent of total global pharmaceutical developed, based on the mammalian cells, or transfect, using plasmid sales. Much of the pharmaceutical industry’s ability to produce any protein DNA, resulting in transient expression of the research is geared towards developing novel protein. The DNA plasmids do not integrate using modern biotechnology biologics. The majority of new biologics are into the host cells’ genome, but accumulate techniques. large proteins that have been modified with episomally and protein expression can the addition of complex carbohydrate chains occur until plasmids are cleared from the to the protein backbone. cells. Transient mammalian cell expression Because of the size and complexity of systems, such as HEK-293, have been most biologics, they need to be produced engineered to produce sufficient protein in cultured mammalian cells that mimic for early stage testing and evaluation of processes that occur in the human body. therapeutic potential. The lack of such a The cells grow slowly and have relatively transient expression system in CHO cells low productivity, resulting in high production has been a limitation. AIBN research has costs. The AIBN building was constructed further developed a CHO-based transient with specialist areas suitable for producing expression system called Epi-CHO to biologics, where mammalian cells can promote episomal plasmid replication and be grown in sterile areas to minimise the plasmid segregation on cell division so the chance of contamination. cells can transiently express proteins for several weeks, rather than only days. AIBN research involving Director Professor Peter Gray has been geared towards In 2010, Joe Codamo, Dr Trent Munro and developing improved mammalian cell Professor Gray published transient CHO lines for producing biologics. The expression data with the highest productivity Chinese hamster ovary (CHO) cell line is of a monoclonal antibody reported globally most commonly used for research and to date. Episomal replication was shown to allow cultures to grow and still maintain high levels of protein expression, and with the Epi-CHO system it was possible to have a final culture volume six times that of the original starting volume. Patents covering the Epi-CHO invention have been issued in the US, Europe, Singapore and Australia. The technology is being commercialised by Acyte Biotechnology Pty Ltd, a UQ and University of NSW spin-off company. In stable protein expression systems, the plasmid coding for the protein of interest is integrated into the host cell genome, so the host cell and its progeny will produce the protein of interest. Stable expression systems are used to produce clinical grade biologics during product manufacturing.

12 It usually takes six to nine months to While bioprocess optimisation has produce stable mammalian cell lines, facilitated significant increases in the level DSM Biologics comes to Brisbane needed for complex biologics. of recombinant biologic production, there Construction of the $345 million remains untapped potential for rational AIBN research has examined approaches Translational Research Institute is in cell line engineering approaches. Using a to speed the isolation of highly expressing progress at Princess Alexandra Hospital, combination of metabolomics, fluxomics, and clones using fluorescence activated cell Brisbane. It includes a mammalian cell targeted genetic manipulation using zinc finger sorting (FACS) and automated robotic good manufacturing practice production nuclease technology, Dr Munro is collaborating imaging. Such an approach is particularly facility that will be capable of producing with AIBN’s Professor Lars Nielsen to produce useful when the desired protein is a clinical-grade biologics ready for clinical the next generation of host cells for biologics multi-chain protein, such as a monoclonal trials. The concept of having the ability to production. Using a systems biology approach, antibody. The DNA coding for one chain produce clinical-grade material alongside it is possible to model ‘in silico’ cells in of the antibody is placed in a plasmid TRI has been advocated from the outset culture to identify genetic pathways that and attached by a sequence called an by the project champion, UQ’s Professor can be modified to provide more efficient attenuated IRES to a fluorescent marker, Ian Frazer. The Queensland Government recombinant protein production. Preliminary whereas the DNA coding for the other chain established BioPharmaceuticals Australia work has attracted significant commercial is placed in its plasmid and attached by an Pty Ltd, with Professor Frazer and AIBN interest and a research collaboration with a attenuated IRES to a second fluorescent Director Professor Peter Gray on the board, large US biologics company. marker. The host cells can be transfected to design and build the $60 million facility. with the mixture of the two plasmids and a Mammalian cell research at AIBN has In 2010, it was announced that DSM very small percentage of the host cells will developed rapidly, complemented by the Biologics had been selected to run have the plasmid DNA stably inserted into strong National Collaborative Research the facility, bringing with it high-level their genome. Infrastructure Strategy biologics group under international contract manufacturing the direction of Dr David Chin. AIBN is well That is where the power of FACS comes experience and credibility. That will ensure set up with equipment, such as FACS for in, as it can scan 70,000 cells a second to protocols, procedures and quality control at rapidly isolating highly expressing clones, look for the very few cells with DNA inserted the facility will be acceptable to regulatory and complementary robotic cell selection into the genome to give the correct ratio authorities, such as the FDA in the US, the equipment, the ClonePix. Such equipment of the two chains. It can also allow any EMEA in Europe and the TGA in Australia. complements the extensive suite of cloning, cells expressing both chains at high levels cell growth, and protein purification facilities DSM is an experienced global contract to be sorted from the bulk population and and the protein characterisation equipment manufacturing organisation. The parent grown for further study. Warren Pilbrough’s built up by AIBN Professor Anton Middelberg company has an annual turnover of $10 research at AIBN has shown the power of and his colleagues. billion. Senior DSM Biologics personnel the technique, with its ability to analyse have been quoted as saying one reason cultures cell by cell, to gain a much greater AIBN staff members work closely with they were attracted to the Brisbane facility understanding of what is happening at colleagues from contract manufacturers was the mammalian cell expertise existing the cellular level and the high degree of DSM Biologics and BioPharmaceuticals at AIBN. stochastic variation existing in supposed Australia, who are involved in building the monocultures. new DSM Biologics plant in Brisbane.

13 Metabolic engineering through systems and synthetic biology

Industrial biotechnology offers many advantages over conventional petrochemical production. Biocatalysis enables highly specific synthesis of complex chemicals and fuels from simple sugars with minimal losses. The system is engineered at micron scale (single cell) and readily scaled up in simple tank reactors using a benign solvent (water) at room temperature and low pressure. Until recently, however, industrial biotechnology was limited to products naturally produced at high levels.

Metabolic engineering – the purposeful profile in the narrow field of metabolic design of living organisms for producing engineering of biopolymer production to desired chemicals and fuels – has changed create a comprehensive, internationally that. In the past decade, metabolic competitive metabolic engineering engineering has evolved from crude program. The process was facilitated by retrofitting using genetic engineering the appointment of Professor Sang Yup towards greenfield designs using systems Lee from the Korea Advanced Institute of and synthetic biology. Organisms used in Science and Technology (KAIST) as AIBN’s producing the next generation of bio- inaugural visiting professor. Professor Lee chemicals are being purpose designed. is one of the world’s leading metabolic engineers and spent one year full-time and From the outset, AIBN identified metabolic a second year part-time at AIBN. Professor engineering as a pillar of growth with an Nielsen also spent half a year at KAIST. important nexus of local value, given Queensland’s large sugar industry, and The strategy was backed with substantial international significance. The strategy investments in infrastructure and foundation focused on leveraging group leader research projects by the Queensland Professor Lars Nielsen’s strong international Government Smart State Fund, the CRC

14 for Sugar Industry Innovation through “Ultimately, we hope to Biotechnology, the National Collaborative measure the success of Research Infrastructure Strategy (NCRIS), the metabolic engineering Australian and international companies and program in terms of UQ. From 2006 to 2009, about $3 million industrial biotechnology in infrastructure and $5 million in research companies established in grants were invested. Queensland,” Professor Nielsen said. The year 2010 was a break-through one for the initiative. Two companies that engaged “Facilitating linkages between international early – Metabolix and Dow – signed new, biotech and chemical companies and larger contracts, while several pilot projects Queensland companies is an important role were successfully completed and expected of the program.” to lead to new contracts in 2011. In total, Most recently, the Queensland Sustainable more than $10 million in new research Aviation Fuel Initiative, headed by AIBN, contracts was secured. Several key findings is bringing together aviation companies were published, including the first ever Boeing and Virgin Blue; sugar producer genome scale model for plants quickly Mackay Sugar; oil industry representative followed by the first model for C4 grasses, IOR Energy; and leading international such as sugarcane, sorghum and maize, biotech company Amyris to evaluate and and the first genome sequence and model ultimately develop sustainable solutions to for a sucrose using E. coli. The team was aviation in collaboration with researchers at invited to present work at key conferences UQ and James Cook University. in the US, Korea, China, Europe, Brazil and Australia and the work of several team The timing is right for a major infrastructure members was recognised through awards boom in Queensland industrial and research fellowships. biotechnology.

15 Nano developments for diagnostic advances

Developments in nanotechnology are helping AIBN researchers make rapid advances in medical diagnostics, with benefits for early detection and personalised treatment of diseases such as cancer, malaria and dengue fever.

Professor Matt Trau is at the forefront field was focused on discovering new Battersby being appointed the company’s of developing flexible molecular reading biomarkers that could be used to detect general manager. systems to accurately and cheaply detect diseases. Now, with tens of thousands of Dr Darby Kozak has been primarily involved cancer, which he hopes will replace promising biomarkers discovered worldwide, in Professor Trau’s lab in developing a variety of complicated tests using a there is a dual need for validation of the nanodevices for detecting protein-based range of expensive equipment. He has biomarkers and for suitable nanodevices biomarkers and advancing new elastic made major progress in miniaturising to perform the validation work rapidly, nanopore technology as a novel means many of the present diagnostic molecular inexpensively and accurately. of performing nanoparticle and biomarker processing steps onto the surface of a Through Professor Trau’s international readouts, in collaboration with New Zealand single nanoparticle. Professor Trau has also collaborations, his research group has company Izon Pty Ltd. devised novel methods to barcode these gained access to a range of clinical samples particles so each one can be used for a Dr Ashley Connolly has been developing which has enabled testing and refining of different diagnostic test. nanodevices for detecting DNA-based nanodevices being developed at AIBN for biomarkers and working on new methods That means quick, accurate diagnosis, diagnostics. for DNA amplification. hopefully when a disease such as cancer Professor Trau’s group covers a host of is in its early stage and most responsive to Research achievements and advances from research areas, including nanotechnology, treatment. Professor Trau’s group have resulted in genetics, proteomics and molecular more than 60 refereed journal publications “Unfortunately, current diagnostic protocols diagnostics. in the past seven years, along with eight typically depend on a complicated variety Associate Group Leader Dr Bronwyn patents and several important commercial of tests based on a wide range of different, Battersby’s research focuses on developing spin-offs and engagements. As well as and often expensive, technological biosensor technologies for molecular creating Nanomics BioSystems Pty Ltd, the platforms,” Professor Trau said. detection of cancer and infectious disease group has engaged with companies such as “Each different platform requires significant biomarkers. Merck, Genetech, Digene, Beckman Coulter, investment in single-use equipment and The biosensors are platform technologies Panbio and AI Scientific. training. Despite this investment, results can tailored for applications such as reading be ambiguous and require multiple, different The group’s research achievements have epigenetic signatures in breast and cervical tests to produce a confirmed result for a enabled the lab to secure more than cancer, and leukaemia; profiling the single pathogen. $20 million in competitive national and specificity of proteolytic enzymes associated international research funding during the “Nanotechnology offers the promise of with West Nile virus and dengue fever; past seven years. A $4 million Queensland miniaturised, inexpensive, flexible and detecting ovarian cancer protein biomarkers Government National and International robust ‘plug-and-play’ molecular reading in blood; and detecting DNA mutations in Research Alliances Program grant partnered systems that can be effectively deployed in cancer. Professor Trau’s lab with leading biomarker the field.” The biomarkers are being commercialised researchers from the Fred Hutchinson There has been rapid advancement in the by spin off company Nanomics Cancer Research Center, the University of field in the seven years since AIBN was BioSystems Pty Ltd. Her involvement in Washington and the Seattle Biomedical established. At that time, the research the commercialisation activity resulted in Dr Research Institute.

16 Research achievements have also been recognised through numerous awards to staff, such as an ARC Federation Fellowship, a Paul Harris Fellowship and a Pink Circle Breast Cancer Research Award to Professor Trau; and a Merck Fellowship and Smart State Fellowship to Dr Simon Corrie, who trained in his lab. Dr Krassen Dimitrov is working to improve accuracy in diagnosis, with a focus on determining marker molecules associated with tropical infectious diseases, such as malaria and dengue fever. His pioneering concept of direct digital counting (DDC) of single biomolecules has demonstrated sizable advantages in sensitivity and accuracy over classical, dose- responsive detection of analogue signals. That means single-molecule nanolabels can be used to determine marker molecules in small amounts of biological samples and provide an accurate diagnosis. The nanolabels are translocated with magnetic forces through a nanometer-sized electronic sensor for digital readout. Dr Dimitrov’s concept of a single-molecule labelling to a portable electronic format points to a new way of diagnosis. “Where this whole thing is headed is rapid, hypersensitive, point-of-care diagnostics with digital accuracy. In other words, tests with high sensitivity and accuracy that can be performed in a GP’s office,” he said. Developing the diagnostic platform is a multidisciplinary endeavour, requiring integration of knowledge from electrochemistry, nanofabrication, electronics and electrical engineering, nanoparticle science, physics of magnetic fields, biochemistry, bioconjugation, surface chemistry and polymer chemistry. The research is loosely segmented into three components. There is the design, synthesis and characterisation of a single-molecule nanolabel, consisting of a magnetic particle, a non-magnetic particle, flexible DNA-linkers and affinity probes. Then there is nanosensor work featuring flat CMOS- compatible 20-50nm nanoelectrodes. Finally, there is the magnetic assembly for magnetophoretic translocation of the single- molecule labels to the nanosensor. Following success in commercialisation with Dr Dimitrov’s single-molecule start-up company NanoString Technologies, which has been placing DDC technology all over the world, AIBN hopes to develop his latest research for the market.

17 Collaboration advances energy research

Interdisciplinary and international collaboration have been the cell technology through crystal engineering hallmark of many important scientific breakthroughs at AIBN, of titania single crystals and the viability of supercapacitor technology for high power including developing alternative renewable energy sources. density and capacity has increased through nanostructured carbon research. Major UQ Deputy Vice-Chancellor (Research) breakthrough photocatalytic catalysts have and AIBN Group Leader Professor Max been developed with promising applications Lu and his researchers are working on in environmental pollution control. various energy conversion and storage Photocatalysis is an important chemical systems with particular focus on optimising process that underpins development of the physical and chemical properties of critical environmental and renewable energy nanomaterials. technologies, such as photocatalytic water Since being established seven years ago, purification, hydrogen production from splitting AIBN has made notable advances in solar water and highly efficient, low-cost solar cells.

18 The search for more efficient photocatalysts Because photocatalysts underpin many The group has developed high-performance has intensified in the past decade because renewable energy industries, the research has phosphate adsorbents for use in water of their great significance in clean energy great potential to lead to economically viable treatment. The group is also developing a and environmental applications. AIBN alternative energy sources, including highly new generation of anti-cancer nano-carriers research has focused on design and efficient and cheap photovoltaic cell systems. and cancer diagnosis tools for cancer fabrication of nanostructured materials for AIBN has made a global contribution in detection and therapy. sustainable energy conversion applications, the field during the past seven years, with “The next stage of our research is to get including efficient photocatalysts for water cross-discipline collaborations, papers in deep fundamental understanding, through and air purification, solar cells, self-cleaning high-ranking research journals and the which we can design better materials in coatings and water splitting for hydrogen number of invited keynote addresses delivery; cancer diagnosis and therapy; and production. The measure of outstanding presented by the institute’s clean energy water treatment,” Professor Yu said. achievements in the field is highlighted with researchers a testament to the recognition the research on novel photocatalysts for AIBN Group Leader Professor John achieved in this dynamic field. hydrogen production being published in Drennan and his group are participating Nature Photonics in May 2011. Nanostructured materials, such as porous in a fruitful international collaboration with carbons, nanotubes, oxides nanoparticles the National Institute for Materials Science, In 2008, Professor Lu and his research and nanofilms, have been developed to offer in Tsukuba, Japan. Professor Drennan’s group published breakthrough results in tremendous potential in electrodes, energy group collaborates with Professor Toshiyuki the publication Nature, describing their storage media, catalysts and membranes for Mori and his researchers, developing a new theoretical computational studies and clean energy production, and CO separation way of looking at materials important in experimental techniques to synthesise a 2 – all important for a clean energy future. developing fuel cell technology. highly reactive and efficient photocatalyst. According to the ISI Web of Science Research from Professor Lu’s group in Solid oxide fuel cells that operate at about database, the Nature paper has since been those fields includes clean energy and green 750°C are extremely efficient at consuming cited 199 times, highlighting its impact on chemistry, under the leadership of Dr Jorge fuels ranging from hydrogen to natural the scientific community. Beltramini. His team’s interest includes gas. At the heart of the fuel cell is a dense the synthesis, manipulation, and physical, ceramic electrolyte that can conduct oxygen The Nature paper demonstrated the chemical and catalytic characterisation of collaborative strength of AIBN, with Professor ions. The ceramic electrolyte must have new nanomaterials for natural gas, coal, and good mechanical strength at the operating Lu co-authoring the work with research biomass conversion into valuable chemicals group members Associate Professor temperature and be chemically stable for and transportation fuels; clean hydrogen the life of the fuel cell. Shizhang Qiao, Huagui Yang and Gang production; reformulation of heavy residues; Liu; Chinese Academy of Sciences Dr Hui waste plastic degradation into fuels; and “The challenge is that the present generation Ming Cheng; AIBN Group Leader Professor heterogenisation of homogeneous catalysis of electrolyte materials has a tendency Sean Smith; computational scientist Dr for fine chemicals production. to ‘age’ over time. The materials are Chenghua Sun; and Centre for Microscopy & doped ceramics and the dopants tend to AIBN’s Dr Denisa Jurcakova has researched Microanalysis Professor Jin Zou. phase separate under fuel cell operating carbon and nanotechnology, specialising in conditions,” Professor Drennan said. Professor Lu’s group, including Associate nanostructured carbon for supercapacitors. Professor Qiao, successfully engineered the As part of Professor Lu’s group, Dr Jurcakova That results in reduced efficiency through a surface and crystallographic characteristics has a team developing several highly efficient drop in the ions’ conductivity and influences of crystalline materials to synthesise uniform nanostructured carbons from various various interfaces that make up the total fuel anatase titanium dioxide (TiO ) single 2 low-cost precursors. Novel insights into the cell package. The ageing of the electrolyte crystals with a high percentage of reactive fundamental understanding of the effect of material manifests itself through subtle surfaces, using hydrofluoric acid as a pore size and surface chemistry of carbons on changes in the material’s microstructure morphology controlling agent. their energy storage capacity were obtained when observed at the atomic level, which The fluorated surface of the single crystals in a very productive collaboration with the researchers have called microdomains. Professor Teresa Bandosz, of City University can easily be cleaned using heat treatment to “Microdomains are found throughout render a fluorine-free, high-purity anatase TiO of New York. Profound understanding of the 2 the family of electrolytes, irrespective of surface without altering the crystal morphology. energy storage mechanism in nanoporous composition, and appear to change over Inorganic single crystals with highly reactive carbons is critical for developing high time, consolidating into separate phases. surfaces have long been studied because of performance supercapacitors for many That change in the microstructure causes their scientific and technological importance applications, including hybrid vehicles and deterioration and an understanding of the as photocatalysts. But surfaces with high renewable energies. process will allow us to control the initial reactivity usually diminish rapidly during the AIBN Group Leader Professor Chengzhong microstructure to minimise the effects of crystal growth process. (Michael) Yu and his team are working ageing,” Professor Drennan said. on the synthesis and characterisation of A typical example is anatase TiO2. Most novel nanomaterials that can be used “Our understanding of the microstructure, available anatase TiO2 crystals are dominated by thermodynamically stable surfaces, rather in biotechnology, clean energy and coupled with conductivity measurements, than more reactive surfaces, which account environment protection. has given us a model of the process and we now have a predictive method that explains for only three percent of the total surface. Funding from the Environmental how the microdomains develop over time. The work of Professor Lu’s group illustrates Biotechnology Cooperative Research AIBN’s influence as a multidisciplinary Centre and the Australian Research Council “By understanding the evolution of the institute to enabling combining theoretical has given Professor Yu and his team the microstructure, we have the first steps computational studies and experimental opportunity to consider fundamental and in understanding the mechanisms of techniques to achieve engineering of application-driven research, important conduction, which gives us a means of surface and crystallographic characteristics for translating cutting-edge science and improving and controlling the materials’ of crystalline materials. technology into commercial products. properties,” he said.

19 Novel polymers and nanostructures for new generation of products

AIBN’s novel polymer and nanostructure research spans a broad reaction SET-NRC. His group developed spectrum of potential benefits, such as earlier detection of diseases; a dual ‘click’ reaction that could direct the pathway of forming complex polymer bio-based polymers for applications including packaging; biomedical architectures by modulating the catalytic advances; biocompatible polymers; and nanomaterials that can activity of each ‘click’ reaction. That meant outperform many existing commercially available materials. third generation dendrimers could be rapidly produced with very high efficiency in less than 30 minutes at room temperature. Ground-breaking research in polymer Showing the diversity of Professor Professor Monteiro’s research group architecture, biomaterials, polymer Whittaker’s group, the researchers are has also developed novel synthetic chemistry and nanocomposites has also world leaders in new materials methodologies to prepare cyclic polymers advanced beyond pure research with for manufacturing faster, more efficient and various cyclic topologies. The cyclic many developments very close to computer processors, attracting funding polymers have very interesting physical and commercialisation. from major manufacturers in North America behavioural properties. His contribution to and research groups globally. The work in Professor Andrew Whittaker’s group is those areas has been well recognised with polymers for integrated circuits has strongly at the forefront of applying fundamental an average of 30 citations per paper. influenced directions adopted by the chemical knowledge to biology and microelectronics industry, leading to some He has been at the forefront of major nanotechnology, placing it in a perfect technologies being cancelled and others scientific breakthroughs in the synthesis of position to attract interest from international adopted. Faster, more environmentally polymer nanostructures in water, leading to collaborations and commercial partners. sound computers are the end result. controlled particle size, structure, molecular The group works in a dynamic field that weight and chemical composition. That has The group’s research achievements has advanced enormously since AIBN was opened avenues for new drug and vaccine have been recognised with a prestigious established, starting with exploration of major delivery carriers, stem cell ‘on-demand’ Australian Research Council (ARC) advances in synthetic chemistry: controlled scaffolds, nanoreactors for organic and Australian Professorial Fellowship for radical and click chemistries for manufacturing polymer reactions, paints, and pressure- Professor Whittaker; an ARC Future complex, predictable molecules. The field has sensitive adhesives. The work led to high Fellowship for Associate Professor Idriss become more diverse. The scientific challenge impact publications in journals including Blakey; and a University of Queensland now is to translate chemical knowledge to Nature Nanotechnology, Angewandte Research Excellence Award for Dr Kris biological systems. Chemie and ACS Nano. Thurecht. Meeting the challenges in biomaterials, He was the first to prepare well-defined Polymer chemist Professor Michael Professor Whittaker’s research group has polymer nanoparticles with controlled Monteiro is recognised as a world leader introduced new concepts in molecular molecular weight by emulsion and in the field of ‘living’ radical polymerisation imaging and proposed novel materials miniemulsion, and the first to prepare to produce highly complex polymer for drug delivery and tissue regeneration. complex core-shell block copolymer architectures and nanostructures in water. With the help of national and international nanoparticles via reversible addition- He recently was awarded the prestigious collaborators, Professor Whittaker’s group fragmentation chair transfer (RAFT) in Future Leadership Award by the Australian is applying fluorine magnetic resonance emulsions. Davos Connection. Professor Monteiro was imaging agents to detect Alzheimer’s one of the first researchers to synthesise That means Professor Monteiro provided disease; forms of cancer including complex polymer architectures using a critical mechanistic knowledge for the melanoma, breast and prostate cancer; and combination of ‘living’ radical polymerisation design of new nanoreactors that produced malignant gliomas – tumours that start in and ‘click’ chemistries. His work has also polymer particles with unprecedented the brain or spine. The advances will lead to led to the new ‘living’ radical polymerisation control over both the particle size early detection of disease and the ability to technique SET-LRP and the ultrafast ‘click’ distribution and the molecular weight repair damaged, unhealthy tissue.

20 distribution. His work has, for the first time, Associate Professor Darren Martin’s overcome many barriers of conducting research group has progressed to RAFT in dispersion polymerisation, and has commercialising nanotechnology-enhanced led to significant industrial interest. rubbers (polyurethane nanocomposites) that outperform existing, commercially available Professor Monteiro has developed a novel materials in many industrial and biomedical method for creating micelle structures applications. by using a mechanical stimulus. Many well-known structures, such as cylinders, The group has engineered plate-shaped spheres and vesicles, can be made nanoparticles, about one nanometre thick instantaneously and in quite high amounts – and up to several hundred nanometres in a significant improvement on traditional self- diameter, and found clever, fully scalable assembly methods. The structures retain ways of incorporating small amounts of the their morphology after freeze drying and are particles into polymers – typically making up in a trial as ‘on-demand’ scaffolds for stem only 2 percent of the weight of the resulting cells and drug and vaccine delivery carriers. nanocomposites. “This is an exciting period for my group, as That ensures the nanoparticles add strength we use our polymer nanostructures for many and barrier, dimensional stability or thermal important biomedical and other industrial properties, without compromising flexibility, applications,” Professor Monteiro said. transparency or processability. Applications for AIBN Group Leader Associate Professor Martin said the work Professor Peter Halley’s work include starch- demonstrated a thorough, systematic based polymers for dengue fever control, approach to “fine tuning” very complex new lignin polymers for barrier applications, multiphase systems. and polymers from wastewater streams. With input from Dr Grant Edwards and Dr Group researcher Dr Bronwyn Laycock is Anthony Musumeci, the work led to several focused on low-cost polyhydroxyalkanoate patents and patent applications that form polymers from wastewater and degradable the intellectual property foundation of AIBN agricultural films. spin-off company TenasiTech Pty Ltd. Professor Halley’s group has the ability Although many researchers have to work on large, Cooperative Research investigated polymer nanocomposites, Centre-funded projects with 20-person few academic teams have expanded their research groups, including government, technologies through to industrial-scale university and industry research and manufacture while maintaining all the development personnel, which means benefits demonstrated at laboratory scale. larger goals are achieved faster. “The work also showed me that achieving effective In late 2010, Associate Professor Martin’s product development work required a group produced more than a tonne of combination of cutting-edge research work; material and demonstrated world’s best applied scale up and technology transfer performance levels. development; and project management Associate Professor Martin said the skills,” Professor Halley said. innovation and commercialisation The work builds on the experience of process sometimes challenged university- Professor Halley’s success in a previous based academics’ traditional roles and role in setting up spin-off company perspectives. Plantic Technologies, which has launched All TenasiTech group members had been biopolymer products such as chocolate and heavily involved in market and customer biscuit tray packaging. Dr Celine Chaleat is interactions, technology intelligence and working to develop water-resistant starch information-sharing activities. polymers. Other researchers from Professor The activities were not typically associated Halley’s team are working on lignin with “a day in the life of an academic”, polymers, aimed at replacing box coatings but the professional development they in industrial packaging and furniture’s resin generated was invaluable. coatings. In the future, bio-based polymers will be used in biomedical applications In the future, the team’s nanomaterials for scaffolds and biodevices and capsule could appear under car bonnets, in solar design for drug delivery. cells, in bullet-proof glass or even in next- generation cochlear implant devices. Professor Halley said the next stage of the work required improving key properties. The technology’s commercialisation was “Improvements in water resistance, close. “We are finalising large trials with mechanical properties and programmed major materials suppliers and end users. degradation may be achieved by novel Within two to three years we hope to see technologies like reactive processing, industrial products made from TenasiTech’s polymer blending and coating technologies.” super-tough elastomers.”

21 OUR VISION

To build a nationally and internationally acknowledged bioengineering and nanotechnology institute recognised for sustained research excellence, with strong collaborative links to leading global research groups and corporations.

22 engagement

Fast facts – Four start-up companies based on AIBN discoveries and innovations. – Total patent families managed in 2010 was 58. – AIBN has more than 150 international collaborations. – Independent review from the Allen Consulting Group confirms AIBN is a significant contributor to Queensland’s economy.

23 New dow Partnership

AIBN has extended the relationship with Dow Chemical Company in 2010 to focus on researching sustainable sources for chemicals, new- generation circuitry for electronics and improved energy storage systems.

AIBN and Dow Chemical Company signed manufacturing integrated circuits for use in areas such as alternative energy, affordable a Memorandum of Understanding (MOU) computers, cameras, smart phones, hand- and adequate food supply, decent housing, in November, with Dow to contribute about held gadgets and even fridges. clean water, and improved personal health $1.74 million in the three-year alliance. This and safety. Professor Nielsen and Dr Jens Kroemer will builds on the success of a relationship use scientific advances in the biosciences to Professor Gray said AIBN had experience in started in 2007, when AIBN and Dow signed genetically reprogram bacteria to produce working with large international companies the first research collaboration in the areas the chemical building blocks of the future. and had flexible arrangements, ensuring of bio-mimicry and a systems biotechnology the institute was responsive to a company’s approach to improving natural pesticides’ AIBN Director Professor Peter Gray said needs and allowed valuable research to be productivity and decreasing the cost. the institute had world-leading researchers, conducted. advanced capabilities and a strong record of The MOU signed in 2010 covers projects industrial collaboration, which ensured the He said the MOU demonstrated Dow’s involving AIBN Group Leaders Professor collaboration with Dow was a good fit. appreciation of the quality of AIBN work Max Lu, Professor Lars Nielsen and and was a powerful indicator of the high Professor Andrew Whittaker. Dow is the second largest chemical estimation of the institute as a research manufacturer in the world by revenue, Professor Lu and Dr Denisa Jurcakova partner for big business. providing plastics, chemicals and will lead a research project into high agricultural products. It has a presence Collaborations such as the one with Dow performance cathode materials based in more than 175 countries and employs gave AIBN researchers a real opportunity on low-cost nanocarbons. The project’s 46,000 people worldwide. It lists innovation to progress their life’s passions beyond objective is to develop improved cathode as the cornerstone of the company’s the confines of the bench to a potential materials with high energy and power success and uses the human element to commercial outcome, Professor Gray said. densities for applications in hybrid vehicles inspire scientists to strive to improve those and renewable energy storage systems. things essential to human progress. Professor Whittaker and Associate Professor Dow’s research and development arm has Idriss Blakey will conduct research into more than 400 projects. Since 2005, annual new-generation circuitry for electronics. patent disclosures have more than tripled. Researchers will use organic synthesis, The company focuses on developing physical chemistry and electrical engineering products and solutions that make to craft functional plastics and polymers for sustainable improvements in the world in

24 aviation fuel for the future

Researchers from AIBN are working in collaboration with world leaders to address issues covering areas as widely divergent as transport, sugar production and environmental sustainability.

Aviation giant Boeing, US renewable While Professor Nielsen will work on the and can be readily integrated with existing products company Amyris, airline Virgin, fermentation and conversion of sugar cane detailed models of sugar mill processes. oil processing company IOR Energy and juice, Boeing will bring its knowledge of Professor Nielsen said the research was Mackay Sugar are working with Professor sustainable aviation fuel and Amyris will important for both the sustainability and Lars Nielsen to develop a sustainable work on the microbial conversion of sucrose continuity of the aviation industry and the aviation fuel industry. to fuel. Mackay Sugar will bring with it health of the planet. knowledge of the sugar industry and IOR Professor Nielsen’s research to identify Energy will conduct final conversion steps “The replacement of petroleum-derived the most efficient method of developing to sustainable aviation fuel. transport fuels with renewable alternatives is industrial processes for producing an important step in mitigating greenhouse bio-derived jet fuel received $2 million in Professor Nielsen’s research will involve gas emissions and ultimately to meet Queensland Government funding. Boeing, systems and synthetic biology to improve transport needs once oil reserves have Amyris, The University of Queensland and the performance of yeast in fermentation. been exhausted,” he said. AIBN jointly matched that amount. Strain performance, covering yield, productivity and titre, is the critical factor Jet fuels account for 5 percent of the The project has started with Professor in cane juice fermentation, since it dictates world’s transport fuel use and 15 percent of Nielsen’s feasibility study of biofuel capital costs. the transport use in Australia. Unlike ground production from sugar. It is part of a bigger transport, where electric or hydrogen cars project, which involves other research Production of sustainable aviation fuel may prove a preferable alternative, aviation investigating the feasibility of using algae through fermentation of cane juice is by far depends fundamentally on liquid fuels with and oil seeds as alternative sources. the best understood of the three processes. high energy content. Fermentation processes are well-developed

25 Fabrication Facilities open doors to industry

Industry partners are continuing to develop their research using Queensland Government, looking into using AIBN facilities and capabilities, including the National Research and sucrose from sugar cane as a feedstock for biotechnological production of chemical Development Infrastructure. AIBN houses the Australian National feedstocks and jet fuels. Fabrication Facility, Queensland node (ANFF-Q); the Queensland The Biologics Facility collaborated with node of Metabolomics Australia; and the National Collaborative more than 20 Australian and international Research Infrastructure Strategy (NCRIS) Biologics Facility. biotech companies and research institutions in 2010. Facility staff worked on more than 100 projects and manufactured more The infrastructure ensures AIBN plays a The staff’s ability to support specialist than 100g of highly purified protein, mainly leading role in supporting Queensland and research is also being recognised at other recombinant antibody, for research and Australian businesses undertaking research, institutions, with partnerships involving early clinical uses. development and innovation. Its expertise the Queensland University of Technology, The facility employs 13 staff with experience and equipment is available to the broader Griffith, the University of Western Australia, in molecular biology, antibody engineering, Australian research community, which the University of South Australia, Flinders bioengineering/bioprocessing and protein includes industry, academia and private University and CSIRO. Staff have also chemistry. In 2010, the staff worked closely organisations. worked with groups from seven diverse faculties and institutes from around The with Queensland Health, CSIRO and Among the organisations developing University of Queensland. scientists from the US to manufacture a their research using the infrastructure high-quality antibody which will be used are ANFF-Q industrial users Intel, Metabolomics Australia expanded its to finalise pre-clinical and animal studies Unilever, Xerocoat Ltd and SkillPro Ltd. collaborative reach in 2010, creating new to determine its efficacy as a potential Metabolomics Australia has industry links around the country and overseas. therapeutic for the deadly Hendra virus. partners such as Dow Chemical Company The facility’s external focus was mainly on The facility plays an important role in and collaborations with the Royal Children’s industrial microbiology, serving industry narrowing the gap between research Hospital in Brisbane and the Australian partners such as the Dow Chemical and clinics, providing expert knowledge Wine Research Institute in Adelaide. Company with systems and synthetic biology services. Staff were involved in a in protein production and state-of-art The three facilities have reported pleasing Bioplatforms Australia sponsored research equipment. Through its subsidised service results in 2010, with a major expansion at project on the systems biology of wine for Australian researchers in protein ANFF-Q; the creation of new national and making, in collaboration with the Australian manufacturing, the facility has also assisted international links at Metabolomics Australia; Wine Research Institute in Adelaide. many start-up biotech companies attract and work on more than 100 projects at the further funding for their drug development Biologics Facility. Facility staff also supported research into programs. health issues, looking into juvenile diabetes in ANFF-Q further developed its capabilities collaboration with the Royal Children’s Hospital AIBN’s national research and development in deposition, device packaging, silicon in Brisbane and investigating the intracellular infrastructure is valued at more than carbide technology and metrology tooling fluxes of Leishmania major, a parasite $30 million and was established with during the year. The facility incorporated causing Leishmaniasis, in collaboration with support from the NCRIS, the Queensland the services and expertise from Griffith the Bio21 Institute in Melbourne. Government and The University of University’s Queensland Micro and Queensland. The expertise and Nanotechnology Centre in 2010, resulting in Internal projects with research groups equipment enables AIBN to conduct a major expansion of the facility’s capability at AIBN included the unravelling of globally competitive research in biologics, in fabrication. metabolism of human embryonic stem metabolomics and nanofabrication. cells in collaboration with the research ANFF-Q also attracted more industrial users, groups of Professor Justin Cooper- with a 50 percent usage increase from White and Associate Professor Ernst 2009 to 2010 and more than 6000 hours Wolvetang. The facility provided key of user access recorded during the year. capabilities in metabolomics analysis The facility’s five staff supported and trained for two major National and International more than 150 users on specialist, state-of- Research Alliances Program grants from the the-art equipment.

26 Continuing professional development

AIBN Associate Professor Stephen Mahler continued to engage with industry through professional development in 2010, designing a course for the biologics industry and attracting audiences in Malaysia and Brazil.

Associate Professor Mahler designed the government are in line with AIBN’s AIBN Short Course in Biologics Research commitment to engaging with industry and Development in 2010 to benefit the around the world. AIBN realises the longer- many stakeholders in the biologics industry. tem benefits of interacting with industry and the wider community and is expanding It provided an appreciation of the activities in the field. many facets of biologics research and development for stakeholders employed Continuing professional development in regulatory affairs; big pharmaceutical allows AIBN to engage with significant companies; small and medium-sized sectors in the economy and support key biotechnology enterprises; patent and professions; provides new access pathways legal services; research institutes; clinical into postgraduate award programs; and, medicine; and aligned organisations. potentially, opens new income streams. The course covered the many facets of biologic medicines, including a global overview of the industry, research leading to discovery, therapeutic modalities (the Biologics are recombinant DNA-derived diseases they treat and how they work), therapeutic proteins, used for treating how they are manufactured, regulatory diseases including cancer, diabetes issues and the future outlook for biologics. and inflammatory diseases, such as rheumatoid arthritis. It followed from previous continuing professional development courses Associate An increasing proportion of Professor Mahler has designed, starting pharmaceutical company revenue is with a successful collaboration with Pfizer derived from biologics, with growth Australia in August 2007. He helped develop in new biologics exceeding that of a three-day biologics course for Pfizer staff. pharmaceuticals. The growth is due to the increasing demand for new biologic Associate Professor Mahler went on to entities and the expiry of several key develop similar courses for Universiti Sains patents, driving the emergence of Malaysia in Penang in 2009; the Malaysian biosimilars – the biologics equivalent of Government’s National Pharmaceutical pharmaceutical generics. Control Bureau in May 2010; and the First National Symposium on Biosimilars at Curitiba, Brazil in November. Associate Professor Mahler’s professional development courses for industry and

27 engaging through affiliates Program

AIBN continues to pursue its mission to engage with industry and has made significant progress in this area in 2010. The Australian- There are three levels of IAP membership, designed to cater for small, medium and first Industrial Affiliates Program (IAP), established in late 2009, has large companies. They are: attracted a range of members from small Australian companies to Industrial Affiliate: This is the large multinational organisations. premium level of engagement with AIBN. Companies receive the greatest level of flexibility when interacting with The IAP was initiated as a formal, non- Brisbane-based contract drug manufacturer institute researchers, which means more project-specific mechanism to bring AIBN PharmaSynth, a subsidiary of Progen opportunities to benefit from AIBN’s ideas, and industry closer together. It provides Pharmaceuticals, joined the IAP because it know-how and capabilities. Industrial industry with an opportunity to access AIBN gave the company access to services it did Affiliate is most suited to organisations research and expertise, while the institute not have in-house. looking to aggressively drive their can improve the industrial relevance of its PharmaSynth chief executive officer Les research and development activities in research programs. Tillack said the ability to access AIBN’s areas where access to state-of-the-art The program aims to build long-term molecular biology facilities meant an facilities and know-how is critical. relationships with companies, promote expanded range of services was available Industrial Member: This membership collaborative research with those to its clients. AIBN’s proximity, relatively has a high level of engagement with AIBN organisations and maintain AIBN’s position attractive costs and a streamlining of and opportunities to interact directly with at the forefront of developing cutting-edge the ability to work with academics were groups and facilities within the institute. technology. positives for PhamaSynth. It is most suited to small-to-medium AIBN Director Professor Peter Gray said “AIBN is keen to work with industry, and enterprises needing to augment in-house it was part of AIBN’s push to “build we can use AIBN in our marketing. It’s a activities with access to knowledge and more bridges between the public and well-known, well-respected brand, so the facilities. private research sectors, expand national relationship is useful to us,” Mr Tillack said. Industrial Associate: This is an entry- capabilities, develop new industries and Wacol-based Very Small Particle Company level membership designed to give promote Australian innovation”. Pty Ltd (VSPC) was one of the first to sign companies an introduction to the breadth This was being achieved through various up to the program in 2010 and re-signed for and depth of AIBN. Industrial Associates means, including the IAP, individual another year in early 2011. have opportunities to interact with AIBN contracts and the National Research and VSPC chief technical officer Peter Talbot research and discuss the range of Development Infrastructure program – a said signing up helped his company services and capabilities available. $30 million cluster of facilities. understand and charaterise nanomaterials. Organisations signing up to the IAP have “We’d be blind without AIBN. It’s integral access to AIBN researchers and facilities; to what we do. Small companies like ours receive invitations to important symposiums don’t have the resources and equipment. and networking events; can receive staff We gain access to library databases and training; and have staff considered for people with current research knowledge.” appointments as adjunct academics. Standard membership fees for the IAP start at $1500 a year.

www.aibn.uq.edu.au/industry-engagement

28 2010 highlights

Fast facts – Prestigious ARC fellowships awarded to six researchers to work at AIBN. – Group Leader Chengzhong (Michael) Yu welcomed to AIBN. – Successful external review of AIBN’s first seven years of operation conducted.

29 Fellowships

Prestigious Australian Research Council (ARC) fellowships were awarded in 2010 to six researchers to work at AIBN, recognising work in cancer detection, drug delivery vehicles and next generation electronics.

AIBN Professor Andrew Whittaker was in finding late-stage tumours, it did not have awarded an ARC Australian Professorial the sensitivity for early detection. Idriss Blakey Fellowship and a Discovery-Project grant. “The MRI contrast agents being developed The funds will help Professor Whittaker’s at The University of Queensland are being research group determine how the design designed to have increased sensitivity to of molecules injected into the blood stream allow for early detection. The smart nature during magnetic resonance imaging (MRI) of the agents has the added advantage that examinations can impact on the quality of they are only visible by MRI when they enter the images received and improve accurate cancer cells, so should reduce the number diagnosis. The work is detailed opposite. of false positives.” Senior researcher at Japan’s National AIBN early-career researcher Dr Chunxia Institute for Materials Science, Professor Zhao was awarded an Australian Ajayan Vinu, was awarded a Future Postdoctoral Research Fellowship to Fellowship to undertake research at AIBN. research better ways to deliver life-saving The fellowship was awarded to Professor drugs. It was a major accomplishment, Vinu to develop a low-cost nanoporous given Dr Zhao was only three years out semiconductor device for the capture and from her PhD at the time and was the sole conversion of CO into fuels by using water 2 investigator on the research project. Aijun Du and sunlight. The novel approach is aimed at delivering low-cost technology offering Dr Zhao will work to develop nanoporous clean energy and helping to mitigate global materials to assist with targeted drug warming. Professor Vinu will take up the delivery. She is working to turn soft emulsion fellowship at AIBN in 2011. materials into nano-porous solids that will help ensure cancer drugs are carried to In recognition of the important work of an affected area, such as a tumour. Such AIBN Associate Professor Aijun Du, the targeted drug delivery aims to reduce ARC awarded a QEII Fellowship, which the seriousness of side effects inherent in formed part of an ARC Discovery-Project broad-spectrum cancer treatments. The grant. Associate Professor Du was also nanoporous materials may do more than awarded an ARC Future Fellowship, which just help with targeted drug delivery, with he declined in order to take up the QEII potential application in photocatalysis for Fellowship. sustainable clean energy. Associate Professor Du’s work involves AIBN’s Dr Jian Liu received an Australian manipulating grapheme materials for Postdoctoral Fellowship for work on a new- smaller, faster and smarter next-generation Jian Liu generation cancer drug delivery vehicle. He electronics devices. Graphene is a film is part of a larger team that secured an ARC of carbon only one atom in thickness. Its Discovery Project grant. The team includes strength, flexibility and electrical conductivity AIBN Professors Max Lu and Chengzhong have opened new horizons, with the (Michael) Yu; and Professor Xinguo Jiang material seen as promising for everything from Fudan University in Shanghai, China. from super-small computers to high- capacity batteries. Dr Liu is combining elements of material chemistry, nanotechnology and AIBN Associate Professor Idriss Blakey was bioengineering to engineer a nano-carrier awarded a Future Fellowship for his work on made of nanoporous silica, designed to smart polymeric contrast agents for MRI. have a high surface area. Dr Liu’s work Associate Professor Blakey said the focuses on preparing nano-carriers, making contrast agents could be used to improve surface modifications, ensuring the pH- the ability to detect cancerous tumours sensitive nano-carrier’s site-specific delivery using MRI. While MRI was already accurate and studying the kinetics of drug release.

Chunxia Zhao

30 We have been working in this specialist field for five or six years to develop a technology we believe has great potential for the early detection of diseases, particularly cancer.

ARC achievement

Professor Andrew Whittaker’s research group came a step closer performed annually worldwide, with about in 2010 to creating a new generation of imaging agents for MRI 30 percent using MRI imaging agents. Imaging agents allow doctors to study examinations, used to detect cancer and Alzheimer’s disease. blood flow and identify particular tissue Professor Whittaker was awarded an Australian Research Council types and diseases. Australian Professorial Fellowship and a Discovery-Project grant to Professor Whittaker said the ARC funds support this work. would accelerate the development of imaging agents and gave the research group the impetus to keep up their hard The funds will help with research on how Imaging, created in 2009 to bring together work. “It is an acknowledgement the work the design of molecules injected into the the skills of a critical mass of researchers and our team has been performing is of the blood stream during MRI examinations state-of-the-art research imaging instruments. highest quality and of great importance. can impact on the quality of the images It is the only facility of its type in Australia and “It is very important. We have been working received and improve accurate diagnosis. one of only a handful in the world. in this specialist field for five or six years Professor Whittaker will work with Centre The AIBN research group aims to design to develop a technology we believe has for Advanced Imaging Principal Research new classes of high-performance imaging great potential for the early detection of Fellow and Associate Professor Stephen agents which offer the prospect of diseases, particularly cancer. Discussions Rose, AIBN Associate Professor Idriss faster, more accurate diagnosis. This will with clinical oncologists have identified the Blakey, and Doctors Hui Peng and Kris involve a broad range of expertise in the enormous potential of the technology under Thurecht, to design new polymer structures polymer chemistry field, including radical development. They are excited by it. for the imaging agents used to detect polymerisation, advanced characterisation “We have initiated several small projects prostate cancer, malignant glioma and methods and magnetic resonance to demonstrate this potential. In addition, Alzheimer’s disease. spectroscopy. discussions are under way with potential Professor Whittaker’s research group is This will be an important step for the commercial partners.” affiliated with the Centre for Advanced estimated 60 million MRI procedures

31 Awards are one measure of the value of researchers and their work at AIBN. The AIBN research community received several awards during 2010, acknowledging the cutting-edge nature of work conducted at the institute. Below are a few of the honours bestowed during the year.

University of Queensland Deputy Vice- AIBN researcher Dr Akshat Tanksale was Chancellor (Research) and AIBN group selected in 2010 for the Australia Japan leader Professor Max Lu was named Emerging Research Leaders’ Exchange International Alumnus of the Year (Research) Program. The Australian Academy of at the 2010 Queensland Education and Technological Sciences and Engineering Training International Awards. The State selected Dr Tanksale on behalf of the Government awards recognised Professor Department of Innovation, Industry, Science Lu for his leadership in research, with a and Research, the Japanese Society for the citation from the judges saying he had promotion of Science, and the Engineering “become a role model, a benchmark, an Academy of Japan. He was one of eight inspiration for other researchers”. mid-career Australian researchers under the age of 45 years selected to visit prominent Professor Mark Kendall’s research team research institutes and universities in Japan won a major award in 2010 for its work in November. on needle-free vaccination delivery device Nanopatch. Winning the 2010 Translational Professor Mark Kendall’s research group Research Excellence Commercialisation was listed as one of three finalists in the Award gave Professor Kendall a chance prestigious Australian Research Council to meet senior executives from global Eureka Prize in 2010. The group was pharmaceutical company Merck Sharp a finalist in the Australian Museum’s & Dohme in the US. Merck Sharp & category Excellence in Research by an Dohme initiated the award together with Interdisciplinary Team. The group made the the Queensland Clinical Trials Network finals for their work on the Nanopatch. to boost the capability of the state’s AIBN students were recognised with a host burgeoning biotechnology industry and gain of prestigious awards and scholarships increased health and economic benefits for during 2010. These are detailed in the Awa r d s Queensland. The award aims to expand Student Experience section of this report. global connections for Queensland science and facilitate more global collaborations for domestic biotechnology companies.

32 Student Experience

Fast facts – Cohort of enrolled Research Higher Degree students reached 100 in late 2010. – Most recent Federal Government figures show growth in PhD commencements at AIBN outpaced all Australian Group of Eight institutions. – Diverse cohort includes students originating from every continent, except Antarctica.

33 RHD report

AIBN’s goal to develop and expand its high-quality student research program has achieved a significant milestone. Student numbers in the research higher degree program reached a new high. The student cohort reached a very healthy 100 in late 2010, with diverse international representation. Females just outnumbered their male counterparts in the PhD cohort by a single student, while numbers were level among MPhil students.

34 Graduations are expected to continue to Chemistry Institute or the New Zealand grow, with Joe Codamo, Drew Titmarsh, Institute of Chemistry. The recognition is Vinh Truong, Guak-Kim Tan and Chalida particularly impressive as Mr Kulis is only in Klaysom expected to have PhDs awarded the first year of his PhD. in 2011. Nani Wibowo won a prize for the best AIBN is conscious of the important presentation in the biopharmaceuticals contributions research students make to the stream of the Asia Pacific Biochemical success of a research organisation and, as Engineering Conference in Kobe, Japan. Ms a result, is committed to providing students Wibowo is working on virus-like particles with a comprehensive scientific skill set, and capsomeres as a vaccine platform. to form the foundation of a successful Nilay Thakar was awarded the prestigious scientific career. International Postgraduate Research The institute encourages and financially Scholarship in UQ’s first scholarship round supports students to engage with research for 2010. The scholarship will cover Mr groups outside AIBN, allowing both Thakar’s tuition fees for the duration of his personal development and skilling and PhD candidature. opening the institute’s door to potential Michael Crichton won the student collaboration and networking with the best category of the Trailblazer Award, minds from around the world. organised by Australia’s leading research It was this culture that enabled PhD commercialisation group, UniQuest. His idea chemistry student Oliver Squires to meet of a micro-needle applicator won because leaders such as former United Nations it was judged innovative and commercially Secretary-General Kofi Annan and anti- attractive. Mr Crichton was a finalist in apartheid activist Archbishop Desmond Tutu the competition, alongside fellow AIBN at a One Young World summit in London researcher Meihua Yu. in 2010. Graduating PhD students set a new Guak-Kim Tan won the International record for AIBN, with eight awarded in Awards and scholarships have assisted Bone-Tissue-Engineering Congress Award 2010 – up from four in 2009. The graduates students to travel and hone their scientific for the best young scientist. The annual were Wendy Chen, Yunyi Wong, Kwok skill sets. The breadth of student successes award recognises an outstanding young Wai Steny Cheung, Esteban Marcellin, in 2010, as exemplified below, recognises scientist who authored and presented at the Wadcharawadee Noohom, Annie Chen, the AIBN cohort’s high-quality research, Bonetec conference with creativity, novelty Lien Chau and Anthony Musumeci. innovation, commercial potential and and clarity. It allowed Ms Tan to complete Dr Marcellin was awarded a Dean’s enthusiasm. Students used those skills to an internship in Professor Yasuhiko Tabata’s Commendation for Outstanding Research give winning presentations and claim prizes Department of Biomaterials lab at the Higher Degree Thesis from The University at leading conferences and awards nights. Institute for Frontier Medical Sciences at Kyoto University in Japan. Professor Tabata of Queensland. It recognised his work in Alexandra Depelsenaire won the NSW PhD is an expert in the fields of biomaterials, the field of understanding molecular weight Student Researcher Award for Excellence in drug delivery system, tissue engineering and control of hyaluronic acid production, for Nanomedicine Research at the International stem cell technology. use in pharmacy, implants, injections for Nanomedicine Conference in Sydney in only osteoarthritis and eye surgery. her first year of PhD studies at AIBN. The Andrew Cameron won the Best Student The Dean’s Commendation gives formal award recognised her work in localised skin Presentation at the 2010 Annual CRC- recognition to outstanding PhD graduates cell death in generating improved immune Polymers Meeting held at CSIRO in who receive unanimous commendations responses during Nanopatch targeted Melbourne. vaccine delivery. from their examiners for genuine and Student Sooa Lim completed a successful substantial contributions to their field of PhD student Jakov Kulis was awarded the year, winning the 2010 Poster Prize Award research. No more than 10 percent of Treloar Prize for best oral presentation at the for a distinguished presentation at the research higher degree graduates are 11th Pacific Polymer Conference. The award Korean Peptide and Protein Symposium in recognised in this way each year. is given to a member of the Royal Australian Seoul on December 3.

35 Mirjana Dimitrijev Dwyer PHD STUDENT PROFILE

Mirjana Dimitrijev Dwyer Australia’s 100 most influential engineers in renewable and do not break down once spends her days in an AIBN Engineers Australia magazine. released to the environment. biochemistry lab while nearing Mrs Dimitrijev Dwyer has set her sights high, “We design peptide or protein sequences the end of her PhD studies. But but believes her time at AIBN has given her which we think will be good surfactants a good grounding to tackle the challenge. and have low production costs, make the she will leave with much more surfactants in bacterial culture, separate “I would like to be involved in the growth them, then test them. By learning which than a highly regarded PhD and of biotechnology in Queensland. I think I elements of the peptide design work or skills in her research field. have things to offer. I have skills across don’t work, we can re-iterate the design the board that you need as a scientist, until we get it right. Specifically, I was Sharing knowledge with leaders at the that you can take to other fields. I have studying the ability of peptide surfactants forefront of research in engineering, learned to plan, write, work with scientists to form viscoelastic skins on the bubble chemistry, biology and computational in other disciplines and work in the research surface, and whether this improves their sciences has broadened Mrs Dimitrijev environment. I have learned to critically performance.” Dwyer’s outlook. analyse difficult situations. I can now “I haven’t just learned to grow cells in a effectively manage myself, my time and The research has instilled in Mrs Dimitrijev shake flask. I listened to what was going other people – and communicate complex Dwyer an appreciation for the importance on all around me at AIBN. The researchers scientific ideas to others.” of commercialisation, in line with AIBN’s specific aim of combining research connect the different fields of science and Mrs Dimitrijev Dwyer was a chemical excellence with an industry focus. The research projects going on around the engineering student at The University of institute’s commercial objectives are building. It definitely opened up things I did Queensland when she made contact with to produce innovations assisting in the not know existed.” Professor Middelberg. She worked with growth of industries; develop new products, Professor Middelberg’s group while writing Mrs Dimitrijev Dwyer began her PhD processes and technologies; and play a in 2007, testing designed peptides as an undergraduate thesis as part of her major role in developing the Australian foaming agents used in cleaning products Bachelor of Engineering. nanotechnology and biotechnology in a bid to produce products not reliant on “I was hearing lots about the projects at industries, through establishing new environmentally unfriendly petrochemicals. AIBN. It sounded exciting because it had opportunities, obtaining the support of She secured a place in Professor Anton real world connections. I talked to Anton the industry sector and interfacing with Middelberg’s research team, which has and he told me about the surfactants.” industrial collaborators. projects in protein and nanoparticle Mrs Dimitrijev Dwyer chose surfactants as Mrs Dimitrijev Dwyer quickly realised being technology for new vaccines; vaccine the topic for her PhD. “Surfactants are a at AIBN opened many doors, given the nano-emulsions; recovery and modification problem for the environment. It is important broad scope of the research conducted and of biopharmaceuticals; design and to know the issue and the ways it can be the network of contacts already in place. bioprocessing of sustainable biosurfactants, solved. She travelled to Daejeon in South Korea; including pepfactants; and nanomaterial “I have been involved in developing an Oxford in the UK; and Knoxville in the manufacture through biomolecular templating. alternative technology to surfactants – the US as part of her studies. She also spent Professor Middelberg is an excellent example active ingredients of most cleaning products, time in the Australian Nuclear Science of the high-quality research supervisors such as shampoos, dishwashing liquid, and Technology Organisation laboratories available at AIBN. He is highly regarded, was and many creams and cosmetics. Just in Sydney, was involved in lodging a awarded a 2010 Queensland Smart Futures like plastics, surfactants are produced provisional patent application and published Premier’s Fellowship and named one of from petrochemical sources. They aren’t in scientific journals.

36 Research skills and problem- across the AIBN website and was quite solving abilities are only a few of impressed by the multidisciplinary nature of Anthony the institute.” the skills graduate Dr Anthony Musumeci has gained during his During a meeting with group leader and Musumeci subsequent PhD supervisor Associate time at AIBN. Professor Darren Martin, Dr Musumeci PHD GRADUATE PROFILE had his first look at the AIBN building and Access to a network of world leaders facilities. in research and cutting-edge facilities attracted Dr Musumeci to AIBN in 2007. “I was impressed by the quality of research, Once he arrived to start his PhD, he also facilities, opportunities and collaborative found a spirit of collaboration, plenty of culture present within AIBN.” support and a chance to travel around the Dr Musumeci also was impressed with world. the facilities and dedicated technicians Now that he has started a new phase on all advanced instrumentation, ensuring of his life, with his graduation in late researchers got the most out of their 2010 and a new role at leading research experiments. commercialisation group UniQuest, Dr While at AIBN, Dr Musumeci was involved Musumeci appreciates how much he in the synthesis and characterisation of a gained during his time at AIBN. range of different nanoparticles (clay and

“AIBN has set me up for a successful career TiO2). “My laboratory experiments were by allowing me to complete a PhD at a not carried out within a single lab in AIBN, respected institution, under the guidance but rather within multiple labs in AIBN, of world-class researchers. The wealth Chemistry and Pharmacology to ensure the of contacts made within AIBN and the best outcomes could be achieved.” research community more broadly will He published five articles in international greatly benefit me in my future career peer-reviewed journals and had the chance endeavours. to travel extensively. “In fact, I would “The research skills and problem-solving have spent more than a year (out of the abilities developed during my PhD greatly three-year candidature) working in different help me in my current role. In addition, laboratories nationally or internationally and in-depth knowledge of the nanomaterials attending conferences. field has been highly beneficial in my current “I had the chance to travel to the USA twice role as a physical science, technology – to attend a conference in Boston and then commercialisation analyst at UniQuest.” to spend four months working at the Center Dr Musumeci had completed an Applied for Nanoscale Materials at Argonne National Science Degree when he came across Labs Chicago. I also had the opportunity AIBN. “I was searching around for a good to attend international conferences in place to undertake PhD studies. I came Switzerland and Amsterdam and visited work colleagues at Cambridge University. “At a national level, I spent six months working at the Australian Nuclear Science and Technology Organisation during eight separate visits. I attended six national conferences in Brisbane, an international conference in Sydney, two international conferences in Melbourne and a national conference in Adelaide.” The collaboration, travel and skilling not only made Dr Musumeci’s time at AIBN memorable, it has instilled a life-long interest in research. “I have learned so much and enjoyed it all. It has given me such a buzz. I hope to stay close to cutting-edge research and make a positive impact for the greater community.”

37 2010/11 Summer Internship

Undergraduate students from The University of Queensland experienced life in an AIBN lab in late 2010. They gained skills for careers in engineering, chemistry and biology as part of AIBN’s Summer Internship Program.

A group of 12 students started their internships in November, with a chance to complete tasks such as sequencing DNA, testing saliva to determine the risks of heart disease and computer modelling exercises. The internship gave students exposure to the research environment; allowed them to explore the possibility of research careers; and displayed the breadth of AIBN’s various research fields. The annual summer internship provides undergraduate students with valuable laboratory experience and exposure to AIBN’s cutting-edge research. It James Briggs is an opportunity for highly motivated undergraduate students to spend eight to 12 weeks undertaking a focused research project. Mr Briggs took part in his second internship the cutting edge, a network of renowned Students assisted in crystallisation studies; in 2009-10 and was still with Associate researchers from around the world and a nanoparticle study involving styrene Professor Wolvetang’s research group in equipment found only in the top research polymerisation; cloning plant genes; early 2011 as a casual research assistant, facilities. while nearing completion in his studies for a synthesising particles for the enrichment AIBN also benefited from hosting interns Bachelor of Science. of peptides; and developing a polymerase such as Mr Briggs, with exposure to the chain reaction-based method for detecting “I have an appreciation of the freedom most promising young researchers at UQ. breast cancer biomarkers. you can have doing research,” Mr Briggs “I have been exposed to what happens at said. “It exposes you to something of They gained valuable research skills; had AIBN and I am feeding that back to other interest to you personally. It links back to access to cutting-edge research facilities; undergraduates,” he said. “That gives my undergraduate studies. Undergraduate received career mentoring; and some them some sense of what happens at the studies have a lot of lecture content but received credit towards an undergraduate institutes. They may then want to test the being at AIBN makes it seem more practical degree. Some students qualified for waters.” scholarships, with The University of and useful for my career.” Mr Briggs hopes to complete his Queensland and AIBN sharing the costs. Another major benefit for Mr Briggs was undergraduate degree mid-2011 and will working with leaders in the research field, AIBN has run a Summer Internship Program then consider eventually taking on PhD such as Associate Professor Wolvetang. Mr for several years, with students returning studies, possibly at AIBN. in subsequent years. One such student is Briggs said he was exposed to research at James Briggs, who first joined the program as part of Associate Professor Ernst Wolvetang’s research group in 2008-09.

38 AIBN’s occupational trainee The experience gained at AIBN was relevant program provided a valuable to the students’ research fields of interest and counted towards the completion of their exchange of knowledge and undergraduate degrees. ideas in 2010, with students AIBN’s occupational trainee program helps from a host of countries working extend collaborative relationships between in the institute’s labs for up to AIBN and renowned institutions around six months. the world and opens opportunities for future funding and further staff and student As many as 28 students from countries exchanges. including China, Germany, France and The program is part of AIBN’s wider Italy undertook occupational traineeships engagement with the global research at AIBN during 2010. They completed community, which includes training people research in fields such as vaccine from universities, research institutes and engineering, fluid mechanics, flow industry in other countries. As part of its simulation, polymer characterisation and development and engagement program, metabolic engineering. AIBN also encourages its own students While the students were at AIBN, they and early career researchers to engage with gained important experience, training and outside groups to develop networks, skills research skills to use in their post-graduate and provide strong foundation networks and studies in their own countries. skills for a successful scientific career. Occupational trainees

39 Research Higher Degree students

Ian Aird Michael Hines Defang Ouyang Samah Alharbi Md Daloar Hossain Ramkumar Palanisamy Eid Alosime Jia Hou Warren Pilbrough AbdulKarim AlSultan James Hudson Amirali Popat Yosephine Andriani Hoai Huynh Clementine Pradal Melisa Anggraeni Sani Saite Jahnke Kun Qian Colin Archer Siddharth Jambhrunkar Lake-Ee Quek Yalun Arifin Pamela Jaramillo Ferrada Anthony Raphael Timothy Brennan Yi Jia Tania Rivera Hernandez Marion Brunck Yan Jiao Suriana Sabri Michele Bruschi Atikah Kadri Anne Sandstrom Maria Buchsteiner Li Pin Kao Miriem Santander Borrego Sandy Budi Hartono Chalida Klaysom Khaled Sebakhy Andrew Cameron Jakov Kulis Abhijit Shrotri Jessica Cameron Kirsten Lawrie Oliver Squires Lien Chau Kebaneilwe Lebani Frances Stahr Annie Chen Pearl Lee Guak-Kim Tan Xiaojing Chen Hui Hui Lee-Wang Nilay Thakar Panagiotis Chrysanthopoulos Peng Li David Thomson Ya-Mi Chuang Ji Liang Kimberley Tilbrook Giuseppe Codamo Wing On Liew Drew Titmarsh Jacob Coffey Soo Lim Thi-Bich-Trinh Tran Natalie Connors Shui Liu Xuan Truong Holly Corbett Chunli Liu Nghia Truong Phuoc Jorja Cork Daria Lonsdale Jennifer Turner Michael Crichton Paul Luckman Srinivas Varanasi Licona Cuauhtemoc Veronica Martinez Salazar Sainimili Vaubula Mateyawa Alexandra Depelsenaire Leila Matindoost Jenny Vo Stefanie Dietmair Stefano Meliga David Wang Mirjana Dimitrijev Dwyer Richard Mills Tianyu Wang Tao Ding Mohd Hezmee Mohd Noor Joshua Watts Yong Ding Marek Mrozkiewicz Nani Wibowo Tania Falzun Sean Muir John Wright Liam Fearnley Dennis Murray Xiao Xia Yan Erika Fiset Geety Nabi Jie Yang Nicholas Fletcher Hoang Quan Nguyen Meihua Yu Marianne Gillard Tuan Anh Huu Nguyen Hidayatul Zakaria Zi Gu Andrew Nolan Bi Yun Zeng Md Haque Huey Wen Ooi Jun Zhang Ryan Harrison Ajay Orpe Yao Zheng Belinda Hartmann Azlin Fazlina Osman Yian Zhu Alejandro Hidalgo-Gonzalez Gillian Osmond

* List includes graduating students and those in a UQ RHD program undertaken at the AIBN during 2010

40 Fast facts – At the end of 2010, AIBN had 19 Group Leaders. – Three AIBN Group Leaders were cited among Australia’s 100 most influential engineers. – Citations for AIBN Group Leaders reached a milestone of 25,000 in 2010.

senior researchers

41 Professor Pe t er Gray AIBN DIRECTOR and group leader

Research: Bioengineering of mammalian cell protein expression and stem cell systems

Large complex proteins that make up • developing and patenting a FACS-based Prowse ABJ, Doran MR, Cooper-White JJ, the majority of biologics approved for system capable of rapidly scanning pools Chong F, Munro TR, Fitzpatrick J, Chung TL, human therapy can only be produced in of cells at up to 70,000 cells per second, Haylock DN, Gray PP, Wolvetang EJ. (2010) mammalian cell expression systems. Such and selecting those few cells from the Long-term culture of human embryonic stem systems can conduct complex assembly population that are expressing high levels cells on recombinant vitronectin in ascorbate and post-translational modification for a of the desired biologic; and free media. Biomaterials 31(32), 8281-8288. molecule’s biological activity. • using modern ‘omics’ approaches to Jones ML, Seldon T, Smede M, Linville A, Mammalian cell expression systems use gain better understanding of cellular Chin DY, Barnard R, Mahler SM, Munster Chinese hamster ovary (CHO) cells as metabolism to allow development of D, Hart D, Gray PP, Munro TP. (2010) A the production host. Although CHO cells host cell lines with improved bioreactor method for the rapid, ligation-independent have been widely used to produce human performance and improved specific reformatting of recombinant monoclonal therapeutics, there are still opportunities to productivity of the desired protein. antibodies. Journal of Immunological improve them as production hosts. Those Methods 354(1-2), 85-90. The research approaches, which have been opportunities include improving properties used to gain a greater understanding of Pilbrough W, Munro TP, Gray PP. (2009) of the host CHO cell line’s ability to grow mammalian cell processes, are now being Intraclonal protein expression heterogeneity at high rates and to high cell densities in applied to developing bioprocesses based in recombinant CHO cells. PLoS ONE 4(12), bioreactor systems, and the ability to rapidly on embryonic stem cells. e8432. doi: 10.1371/journal.pone.0008432. select clones producing the protein of Ladewig K, Niebert M, Xu ZP, Gray PP, Lu GQ. interest at high rates. The challenge with stem cells is to accurately define the physical and chemical environment (2009) Efficient siRNA delivery to mammalian Professor Peter Gray’s research is aimed at cells using layered double hydroxide that allows the controlled proliferation and reducing bottlenecks present when CHO nanoparticles. Biomaterials 31(7), 1821-1829. subsequent differentiation of the cells, then cells are used to produce biologics and translate those conditions into processes Prowse AE, Wolvetang E, Gray PP. (2009) includes: that can be scaled up to produce the A rapid, cost-effective method for counting • developing a CHO cell transient protein number of cells required for clinical testing. human embryonic stem cell numbers as expression system that will allow clumps. Biotechniques 47(1), 599-606. researchers to produce, within a few Key publications for the past five years: Vari F, Munster DJ, Hsu JL, Rossetti TR, days of obtaining DNA coding for the Codamo J, Munro TP, Hughes BS, Song Mahler SM, Gray PP, Turtle CJ, Prue RL, protein of interest, the desired protein M, Gray PP. (2011) Enhanced CHO cell- Hart DNJ. (2008) Practical blood dendritic for characterisation and testing of a new based transient gene expression with the cell vaccination for immunotherapy of potential biologic. The transient CHO Epi-CHO expression system. Molecular multiple myeloma. British Journal of system developed, Epi-CHO, makes Biotechnology 48(2), 109-115. Haematology 143(3), 374-377. use of episomal plasmid replication and plasmid segregation on cell division to Prowse ABJ, Chong F, Gray PP, Munro TP. Gray PP, Pilbrough W, Codamo G, Munro allow the protein to be produced over (2011) Stem cell integrins: Implications for TP. (2008) Speeding up the production several weeks, and has resulted in the ex-vivo culture and cellular therapies. Stem of recombinant proteins by CHO cells. J highest productivity reported for a CHO Cell Research 6(1), 1-12. Biotechnol 136(S1), S8. transient system; Dietmair S, Timmins NE, Gray PP, Nielsen Xu ZP, Niebert M, Porazik K, Walker TL, • high-throughput approaches that allow LK, Kromer JO. (2010) Towards quantitative Cooper HM, Middelberg APJ, Gray PP, Bartlett rapid selection of clones that stably metabolomics of mammalian cells: Develop- PF, Lu GQM. (2008) Subcellular compartment express high levels of the desired ment of a metabolite extraction protocol. targeting of layered double hydroxide biologic; Analytical Biochemistry 404(2), 155-164. nanoparticles. J Control Release 130(1), 86-94.

42 Professor Kirill Alexandrov ARC Future Fellow and Group Leader

Research: Next generation technologies for protein research

Professor Kirill Alexandrov’s research group is developing new methods for rapid in vitro synthesis of proteins and analysis of their structure and function. Advances in life sciences and biotechnology are driven by an ability to replicate the building blocks of life in vitro, modify them and use them in academic and industrial applications. Much biotechnological progress in the past 40 years stemmed from advances in analysis and synthesis technologies for DNA and proteins. However, while orders of magnitude cost reduction was achieved in DNA sequencing and synthesis, the protein technologies have changed comparatively little. Professor Alexandrov’s group is focusing on filling this technological gap with new methods for rapid in vitro synthesis of proteins and analysis of their structure and function. The group has developed a novel, cell-free protein expression system based on protozoan Leishmania tarentolae. It has demonstrated that, using this technology, large sets of genes can be converted into proteins within hours. Group researchers apply the technology to study cellular processes Key publications for the past five years: controlled by Rab GTPases and the role of Kovtun O, Mureev S, Johnston W, protein prenylation in the process. Due to their Alexandrov K. (2010). Towards the importance in many signalling and trafficking construction of expressed proteomes using pathways, a deregulation of the GTPases a Leishmania tarentolae based cell-free (expression defects, mutations, or defects expression system. PLOS One 5(12), e14388. in their prenylation status) is associated with Mureev S, Kovtun O, Nguyen UTT, numerous human pathologies. Alexandrov K. (2009). Species-independent Professor Alexandrov has research projects in: translational leaders enable the rapid development of novel cell-free expression • developing high-yield, cell-free protein systems. Nature Biotechnology 27, 747-752. expression system based on Leishmania tarentolae; Nguyen UT, Guo Z, Delon C, Wu Y, Deraeve • quantitative analysis of protein-protein C, Fränzel B, Bon RS, Blankenfeldt W, Goody protein-small molecule interactions using RS, Waldmann H, Wolters D, Alexandrov K. in vitro protein expression systems; (2009). Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature • proteome-wide analysis of protein Chemical Biology 4, 227-235. prenylation and its variation in human diseases; Wu Y, Tan KT, Waldmann H, Goody SR, Alexandrov K. (2007). Quantitative analysis • understanding mechanisms regulating of the interaction of prenylated Rab proteins protein prenylation machinery; and with REP and GDI explains the requirement • identifying small molecules modulating for both regulators in Rab function. prenylation and localisation of Proceedings of the National Academy of RabGTPases. Sciences USA 104, 12294-12299.

* Joint appointment with UQ’s Institute for Molecular Biosciences.

43 Dr Bronwyn Battersby Associate Group Leader

Research: Developing and commercialising biosensor technology for disease biomarker detection

company is incubated at AIBN. Dr Battersby was recently appointed General Manager. Commercialising Emerging Technologies (COMET) funding was awarded to Nanomics during 2010 for completion of pre-commercialisation activities. Nanomics recently developed its Promenade software with Melbourne- based Bioscience Applications Pty Ltd. Touchscreen-enabled for user-friendliness, Promenade has been designed for rapid analysis of data collected through the use of the biosensor technology in the laboratory.

Key publications for the past five years: Marcon L, Spriet C, Meehan TD, Battersby BJ, Lawrie GA, Héliot L, Trau M. (2009) Synthesis and Application of FRET Nanoparticles in the Profiling of a Protease. Small 5(18), 2053-2056. Chen A, Kozak D, Battersby BJ, Forrest RM, Scholler N, Urban N, Trau M. (2009) Antifouling Surface Layers for Improved Signal-to-Noise of Particle-Based Immunoassays. Langmuir 25(23), 13510- Dr Bronwyn Battersby is working with • reading epigenetic signatures in breast 13515. biomarkers, which are biological indicators and cervical cancer, and leukaemia; Corrie S, Sova P, Lawrie G, Battersby B, for disease found in blood, tissue, urine • profiling the specificity of proteolytic Kiviat N, Trau M. (2009) Development of a and cerebrospinal fluid. Blood components, enzymes associated with West Nile virus multiplexed bead-based assay for detection such as circulating tumour cells, RNA, DNA, and dengue fever; of DNA methylation in cancer-related genes. proteins, peptides and metabolites, are • detecting ovarian cancer protein Mol Biosyst 5, 262-268. potential biomarkers that indicate disease biomarkers in blood; status. Often, more than one biomarker can Corrie SR, Vogel R, Keen IJ, Jack K, Kozak be associated with a given disease. • detecting DNA mutations in cancer; and D, Lawrie GA, Battersby BJ, Fredericks P, Trau M. (2008) A Morphological Study of • diagnosing plant and animal-based There is a strong need to develop Hybrid Organosilica Materials for Colloid- viruses for biosecurity applications. biomarkers and associated molecular Based DNA Biosensors. J Mater Chem 18, diagnostic tests to enable clinicians to The research has been conducted in 523-529. assess for disease risk, personalise therapy, collaboration with the Fred Hutchinson Johnston APR, Battersby BJ, Lawrie GA, monitor response therapy and screen for Cancer Research Center and the University Lambert LK, Trau M. (2006) A Mechanism disease recurrence. of Washington in Seattle; the Peter for Forming Large Fluorescent Organo- MacCallum Cancer Centre; the Garvan Dr Battersby’s research focuses on Silica Particles: Potential Supports for Institute; Queensland Primary Industries and developing and commercialising biosensor Combinatorial Synthesis. Chem Mater 18, Fisheries; the Cooperative Research Centre technologies for molecular detection of 6163-6169. cancer and infectious disease biomarkers. for National Plant Biosecurity; and the Australian Biosecurity Cooperative Research Patents: The biosensors are platform technologies Centre for Emerging Infectious Disease. that have been tailored for several Battersby, Bryant, Trau. Carriers for applications, including: The University of Queensland’s spin-off combinatorial compound libraries company Nanomics BioSystems Pty Ltd (CA2352082-C). Granted September 21, 2010. is commercialising the biosensors. The

44 Associate Professor Idriss Blakey ARC Future Fellow and Associate Group Leader

Research: Functional polymers for value-added applications

Associate Professor Idriss Blakey’s research • maintaining strong links with the Thurecht KJ, Blakey I, Peng H, Squires O, interests span synthesis of functional semiconductor industry, such as with Hsu S, Alexander C, Whittaker AK. (2010) polymeric materials and nanomaterials; and Intel Corp and Sematech (an international Functional Hyperbranched Polymers: advanced characterisation of materials and consortium of semiconductor companies), Towards Targeted in vivo 19F Magnetic surfaces. and developing links with Dow Chemical Resonance Imaging using Designed Company; and Macromolecules. Journal of the American Associate Professor Blakey and his team Chemical Society 132(15), 5336-5337. have key strengths in bringing together these • being awarded an ARC Future Fellowship. two areas by developing a fundamental Active research projects include: Blakey I, Thurecht KJ, Whittaker AK. (2010) understanding of the structure-property High-Pressure Real-Time 129Xe NMR: • smart contrast agents for enhancing relationships for polymers and polymer- Monitoring of Surfactant Conformation performance of Magnetic Resonance based nanomaterials. That understanding During the Self-Assembly of Reverse Imaging (MRI); enables the design and synthesis of Micelles in Supercritical Carbon Dioxide. improved materials for a range of different • high-performance polymers for computer Chemical Communications 46(16), 2850- applications, including biomedical imaging, chip manufacture; 2852. sensors, photolithography and drug delivery. • self-assembly of hybrid nanoparticles for Merican Z, Schiller TL, Hawker CJ, The team and key collaborators cover a biosensors; Fredericks PM, Blakey I. (2007) Self- range of disciplines, including polymer • biodegradable polymers for drug delivery; assembly and encoding of polymer- physical chemistry, synthetic chemistry, and stabilized gold nanoparticles with surface- pharmacy, biomaterials, photolithography, • modification of polymer surfaces. enhanced Raman reporter molecules. spectroscopy, biomedical imaging and Langmuir 23(21), 10539-10545. oncology. Key publications for the past five years: Blakey I, George GA, Hill DJT, Liu Some achievements for 2010 include: Yu A, Liu H, Blinco JP, Jack K S, Leeson H, Rasoul F, Rintoul L, Zimmerman M, Younkin TR, Whittaker AK, Blakey I. P, Whittaker AK. (2007) Mechanism of • initiating an ARC funded program on (2010) Patterning of Tailored Polycarbonate 157 nm Photodegradation of Poly[4,5- development of multimodal biomedical Based Non-Chemically-Amplified Resists difluoro-2,2- bis(trifluoromethyl)-1,3- imaging agents; using Extreme Ultraviolet Lithography. dioxole-co-tetrafluoroethylene] (Teflon AF). • publishing research findings in high Macromolecular Rapid Communications Macromolecules 40(25), 8954-8961. impact/high ranking journals; 31(16), 1449–1455.

45 Professor Julie Campbell NHMRC Senior Principal Research Fellow and Group Leader

Research: Cell biology and tissue engineering

Professor Julie Campbell’s research group discovered that peritoneal macrophages can transdifferentiate into smooth muscle- like cells and that grafts of hollow, smooth- muscle organs (arteries, bladder, vas deferens and uterus) can be grown from those cells in the peritoneal cavity for autologous transplantation. The group’s research is concentrating on the pathways through which the transdifferentiation occurs and their regulatory factors. The group continues to investigate the mechanisms of vascular disease and potential therapeutic strategies. Professor Campbell has research projects in: • gene array analysis of the foreign body response; • macrophages as progenitors of myofibroblasts and their potential as a therapeutic target for fibrotic disease; • regulation of myofibroblast differentiation using lentiviral delivery of shRNA to knockdown genes for candidate growth factors; Key publications for the past five years: Le SJ, Gongora M, Zhang B, Grimmond • antibody-directed delivery of anti- S, Campbell GR, Campbell JH, Rolfe restenotic agents using inorganic Golledge J, Campbell JH. (2010) BE. (2010) Gene expression profile of the nanoparticles (collaboration with Peroxisome proliferator-activated receptor fibrotic response in the peritoneal cavity. Professor Max Lu, Dr Gordon Xu and Dr ligands reduce aortic dilatation in a mouse Differentiation 79, 232-43. Anita Thomas); model of aortic aneurysm. Atherosclerosis 210, 51-56. Gu Z, Rolfe BE, Xu Z, Thomas AC, • the mechanism by which oestrogen Campbell JH, Lu G. (2010) Enhanced mediates its atheroprotective effects on Mooney JE, Rolfe BE, Osborne GW, Sester effects of low molecular weight heparin the vasculature; DP, van Rooijen N, Campbell GR, Hume DA, Campbell JH. (2010) Cellular plasticity of intercalated with layered double hydroxide • the complement system in vascular inflammatory myeloid cells in the peritoneal nanoparticles on rat vascular smooth disease and tumour growth (collaboration foreign body response. Am J Pathol 176, muscle cells. Biomaterials 20, 5455-5462. with Professor Steve Taylor and Dr Trent 369-80. Woodruff, SBMS); and • tissue engineering the kidney. Stickler P, De Visscher G, Mesure L, Famaey N, Martin D, Campbell JH, Van Professor Campbell and Dr Barbara Rolfe Oosterwyck H, Meuris B, Flameng W. gained a new National Health and Medical (2010) Cyclically stretching developing Research Council grant in 2010 to study tissue in vivo enhances mechanical strength the mechanisms of peritoneal fibrosis. and organisation of vascular grafts. Acta Professor Campbell retired in the same year Biomater 6, 2448-2456. as a Senior Principal Research Fellow of the NHMRC after 30 years of continuous funding. She also became President of the Association of Australian Medical Research Institutes for two years.

46 Professor Justin Cooper-White Group Leader

Research: Smart surfaces, scaffolds and diagnostic microdevices for stem cell expansion, tissue engineering and early disease detection

Professor Justin Cooper-White and his George PA, Doran MR, Croll TI, Munro research group are focused on designing TP, Cooper-White JJ. (2009) Nanoscale and developing complex, polymer-based presentation of cell adhesive molecules via structures and device systems that are block copolymer self-assembly. Biomaterials tailored for investigation of biological 30(27), 4732-4737. systems and, in particular, to invoke control Chau L, Doran M, Cooper-White JJ. (2009) over stem cell behaviours. A novel multishear microdevice for studying Projects underway include investigations into: cell mechanics. Lab on a Chip 9(13), 1897- 1902. • engineering surfaces for stem cell attachment and phenotype control; Tan GK, Dinnes DLM, Butler LN, Cooper- • tailored polymeric scaffolds for drug White JJ. (2010) Interactions between delivery and stem cell-based tissue meniscal cells and a self-assembled engineering; biomimetic surface composed of hyaluronic acid, chitosan and meniscal extracellular • cell-based diagnostic microdevices for matrix molecules. Biomaterials 31(23), mapping cellular microenvironments, in 6104-6118. particular cell-surface, cell-cell and cell- scaffold interactions; • microfluidic devices for manufacturing functional microparticles/nanoparticles and probing complex fluid behaviour in those microdevices; and • microfluidic device platforms for biofluid (for example, saliva) characterisation and early disease detection (in particular for cardiovascular disease and cancer).

Key publications for the past five years: Croll T, O’Connor AJ, Stevens GW, Cooper-White JJ. (2006) A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces. Biomacromolecules 7(5), 1610-1622. Rowlands AS, George PA, Cooper-White JJ. (2008) Directing osteogenic and myogenic differentiation of MSCs: interplay of stiffness and adhesive ligand presentation. American Journal of Physiology – Cell Physiology 295(4), C1037-1044.

47 Research: Peptide surfactants and peptide hydrogels

Dr Annette Dexter research interests span • demonstrated the successful design of areas of control of oil-water mixtures and heat-resistant, digestion-resistant mini- peptide hydrogels. proteins for low-cost bioproduction of Dr peptide surfactants. Controlling stability in oil-water mixtures (emulsions) principally involves designing Dr Dexter has research projects in: Annette and testing peptide surfactants that can • peptide surfactants for reversible control stabilise emulsions in high salt, including sea of foams and emulsions; Dexter water and blood serum, with applications in: • biological production of peptide ARC Future Fellow and • drug delivery; concatemers; • crop protection; and Associate Group Leader • specific ion effects in self-assembly and • enhanced oil recovery. colloid stability; and Separately, Dr Dexter has demonstrated • peptide hydrogels for tissue repair and that specific ion effects can be used to drug delivery. control the stability of emulsions formed with conventional, non-peptide surfactants, with Key publications for the past five years: potential benefits in waste water treatment Dexter AF. (2010) Interfacial and emulsifying and disposal of industrial emulsions. properties of designed -strand peptides. Dr Dexter interacts closely with AIBN start- Langmuir 26(23), 17997-18007. up company Pepfactants Pty Ltd, of which Malcolm AS, Dexter AF, Katakdhond JA, she is a founding co-inventor, in developing Karakashev SI, Nguyen AV, Middelberg applications for designed peptides as APJ. (2009) Tuneable control of interfacial green surfactants. In 2010, that involved rheology and emulsion coalescence. sponsored research for a multinational ChemPhysChem 10(5), 778-781. household and personal care company on using designed peptides as cleaning agents. Middelberg APJ, He L, Dexter AF, Shen HH, A separate sponsored project is addressing Thomas RK. (2008) The interfacial structure the incorporation of peptides as green and Young’s modulus of peptide films surfactants in industrial lubricants. having switchable mechanical properties. Journal of the Royal Society Interface 5(18), Work on peptide hydrogels similarly involves 47-54. the design of new peptides, with the aim of forming soft supports for living cells, Dexter AF, Middelberg APJ. (2007) potentially leading to non-toxic injectable Switchable peptide surfactants with implants for new tissue generation, for designed metal binding capacity. Journal example, in repair of the eye or spinal cord. of Physical Chemistry C 111(28), 10484- 10492. Dr Dexter has successfully: Dexter AF, Malcolm AS, Middelberg APJ. • filed two Australian provisional patents on (2006) Reversible active switching of the emulsion stability control and formation of mechanical properties of a peptide film at salt-resistant peptide emulsions; a fluid-fluid interface. Nature Materials 5(6), • demonstrated pH-controlled assembly 502-506. of an elastic hydrogel using a single designed helical peptide; and

48 Dr Krassen Dimitrov Group Leader

Reseach: Single-molecule technologies for diagnostics

Dr Krassen Dimitrov’s research group JL, Peng T, Ratcliffe AL, Webster P J, is working to develop new diagnostic Davidson EH, Hood L, Dimitrov K. (2008) technologies involving single-molecule Direct multiplexed measurement of gene nanolabels that can be used for accurate expression with colour-coded probe pairs. and sensitive determination of marker Nature Biotechnology 26(3), 317-325. molecules associated with disease and Halvorsen OJ, Oyan AM, Bo TH, Olsen S, found in small amounts of biological Rostad K, Haukaas SA, Bakke AM, Marzolf samples. B, Dimitrov K, Stordrange L, et al. (2005) The nanolabels are translocated with Gene expression profiles in prostate cancer: magnetic forces through a nanometer-sized Association with patient subgroups and electronic sensor, for digital readout. The tumour differentiation. International Journal group is developing the technology with a of Oncology 26, 329-336. specific focus on diagnostics for tropical Wang H, Padmanabhan H, Dimitrov infectious diseases such as malaria and K. (2010) Controlled assembling of dengue fever. biomolecule-functionalised magnetic Future applications include such varied nanoparticles. ICBB 2010: International fields as biomedical research, forensics, Conference on Biotechnology and agribusiness, and biosurveillance. Bioengineering. Tokyo, Japan, May 26-28, 2010. Key publications for the past five years: Thomson DAC, Dimitrov K, Cooper MA. Awards and prizes: (2011) Amplification free detection of Nominated in 2009 for the 2010 ENI Award Herpes Simplex Virus DNA. Analyst 136, for Energy Research 1599-1607. Qin G, Darain F, Hui W, Dimitrov K. (2011) Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications. J Nanoparticle Res 13(1), 45-51. Rahaie M, Ghai R, Babi´c B, Dimitrov, K. (2010) Synthesis and characterisation of DNA-based micro and nanodumbbell structures. J Bionanosci 3(2) 73-79. Babic B, Ghai R, Dimitrov K. (2007) Induced movement of the magnetic beads and DNA- based dumbbell in a micro fluidic channel in Proceedings of SPIE: BioMEMS and Nanotechnology III ( Nicolau D V, Abbott D, Kalantar-Zadeh K, Matteo TD, Bezrukov SM, Eds). Babic B, Ghai R, Dimitrov K. (2008) Magnetophoresis of flexible DNA-based dumbbell structures. Applied Physics Letters 92(5). Article number 053910. Geiss GK, Bumgarner RE, Birditt B, Dahl T, Dowidar N, Dunaway DL, Fell HP, Ferree S, George RD, Grogan T, James JJ, Maysuria M, Mitton JD, Oliveri P, Osborn

49 Professor John Drennan Group Leader

Research: Materials development and characterisation

The main body of Professor John Drennan’s Key publications for the past five years: research relates to understanding the Ou DR, Mori T, Ye F, Kobayashi T, Zou J, relationship between microstructural Auchterlonie GJ, Drennan J. (2006) Oxygen characterisation and the physical property vacancy ordering in heavily rare-earth doped of materials. A central focus of the work is ceria. Applied Physics Letters 89, 171911. developing improvements in the conduction of oxygen ions in materials, which has Ye F, Mori T, Ou DR, Zou J, Auchterlonie GJ, application in solid oxide fuel cells. Drennan J. (2007) Compositional and Valent State Inhomogeneities and Ordering of Tight microstructural control at the Oxygen Vacancies in Terbium- doped Ceria. atomic level is proving to be a key factor Journal of Applied Physics 101(11) Art. No. in developing a new range of materials 113528. that have longer operating life and better properties. Yuan P, Liu N, Zhao LZ, Zhou XF, Zhou L, Auchterlonie GJ, Yao XD, Drennan J, Lu The expertise of Professor Drennan and GQ, Zou J, Yu CZ. (2008) Solving Complex his research group in this area has led to Concentric Circular Mesostructures by projects involving other refractory systems. Using Electron Tomography. Angewandte The group is turning its attention to materials Chemie-International Edition 47(35), 6670- for extreme environments and developing 6673. a novel system for protecting critical Yuan P, Zhou XF, Wang HN, Liu NA, Hu YF, components in hypersonic scram jets – a Auchterlonie GJ, Drennan J, Yao XD, Lu GQ, serious challenge for materials science. Zou J, Yu CZ. (2009) Electron-Tomography In addition, the group is examining the Determination of the Packing Structure of development of “zinc oxide (ZnO) soaps” in Macroporous Ordered Siliceous Foams artists’ paints. This phenomenon can be Assembled from Vesicles. Small 5(3), 377- devastating to an artwork, resulting in the 382. formation of large, unsightly protrusions, Knibbe R, Auchterlonie GJ, Mori T, which can crack and severely damage Lashtabeg A, Drennan J. (2010) Glass- the painting. Researchers are examining, Phase Movement in Yttria-Stabilised through very detailed microstructutral Zirconia/Alumina Composites. Journal of the analysis, the mechanism responsible for the American Ceramic Society 93(5), 1494- phenomenon. 1500. Funded through an Australian Research Council Industry Linkage project with major galleries across Australia and several in the US and Asia, the project is looking at the first stages of deterioration of ZnO particles at the atomic level with a view to developing curatorial protocols to minimise damage.

50 Associate Professor Aijun Du ARC QEII Fellow and Associate Group Leader

Research: theoretical modelling for smarter nanoelectronic devices and energy storage

Associate Professor Aijun Du’s research interests span the areas of clean energy, environmental science and nanoelectronics. His research focus is on tackling band-gap problems, charge/spin transport issues, hydrogen storage and carbon-dioxide capture in nanoscale materials. The aim is to develop improved devices for nano- electronics, spintronics, energy storage and conversion. As well as aiming to develop truly smaller, faster and smarter electronics materials, there is the potential for the research to open a new knowledge-based electronics industry and create emerging energy technologies in Australia. Associate Professor Du has strong interactions with disciplines of chemical engineering, environmental science, computational chemistry and condensed matter physics. Associate Professor Du has, for the first time, predicted that lithium-decorated porous graphene is a superb candidate for hydrogen storage. The enhanced adsorption energy and high storage capacity (7 wt %) meets the standard set for 2010 by the Department of Energy The group has research projects in: Du A, Chen Y, Zhu ZH, Amal R, Lu M, in the US. A new mechanism involving • nanomaterials for hydrogen storage; Smith SC. (2009) Dots versus Antidots: Computational Exploration of Structure, electrostatic interaction has been proposed. • nanomaterials for CO capture and 2 Magnetism and Half-metallicity in Boron- Huge magnetism may be associated with activation; Nitride Nanostructures. J Amer Chem Soc triangular nanodots or triangular holes • materials for nanoelectronics and 131, 17354. carved from boron nitride hexagonal spintronics; monolayers and a lattice of such nanoholes • materials for catalysis applications; and Du A, Smith SC, Lu M. (2007) Formation of Single Walled Carbon Nanotube via the demonstrates half metallicity. A collaborator • advanced theoretical modeling methods. is attempting to synthesise such structures Interaction of Graphene Nanoribbons: ab and measure their properties. Key publications for the past five years: initio Density Functional Calculations. Nano Letters 7, 3349. Porous graphene will open a direct gap in Du A, Zhu ZH, Smith SC. (2010) contrast to currently extensively studied Multifunctional Porous Graphene for Du A, Smith SC, Yao XD, Lu M. (2007) graphene. This will provide a novel pathway Nanoelectronics and Hydrogen Storage: Hydrogen Spill-over mechanism on a Pd to solve the formidable hurdle of graphene’s New Properties Revealed by First Principle doped Mg surface as revealed by ab initio lack of “obvious band gap” for building field Calculations. J Amer Chem Soc 132, 2876. Density Functional Theory Method. J Amer effect transistor. Chem Soc 129, 10201. Du A, Chen Y, Zhu Z, Lu M, Smith SC. (2009) C-BN Single Walled Nanotubes from Awards and prizes: Hybrid Connection of BN/C Nanoribbons: ARC Future and QEII Fellowship in 2010 Prediction by ab initio density functional calculations. J Amer Chem Soc 131, 1682.

51 Research: Biofluids characterisation and biopolymer processing

The research interests of Professor Peter Key publications for the past five years: Halley’s group span two major research Liu WC, Halley PJ, Gilbert RG. (2010) areas: Mechanism of Degradation of Starch, a • biofluids characterisation; and Highly Branched Polymer, during Extrusion. • biopolymer processing. Macromolecules 43(6), 2855–2864. Biofluids characterisation incorporates novel Halley PJ, George G. (2009) material and flow characterisation (rheology) Chemorheology; from fundamentals to for a variety of projects, including: reactive processing, Cambridge University Press, Cambridge, UK. • design of texture-modified foods for aged care; Zhao RX, Torley P, Halley PJ. (2008) Emerging biodegradable materials: starch- • cystic Fibrosis sputum diagnostics; and and protein-based bio-nanocomposites. • novel techniques for predicting Journal of Materials Science 43(9), 3058- swallowing of foods and tablets. 3071. The group has strong intereactions with Chaléat CM, Halley PJ, Truss RW. (2008) the disciplines of chemical engineering, Properties of a plasticised starch blend. Part pharmacy, biochemistry, food science and 1: Influence of moisture content on fracture speech pathology, and links with various properties. Carbohydrate Polymers 71(4), hospitals. 535-543. The biopolymer processing area of Russo M, Strounina E, Waret M, Nicholson research focuses on understanding and T, Truss R, Halley PJ. (2007) A study of optimising the processibility of a wide water diffusion into a high amylose starch range of biopolymers such as starch; lignin; Professor blend: The effect of moisture content and polyhydroxyalkanoates (PHA); polylactic temperature. Biomacromolecules 8(1), acid (PLA); and biopolymer nanocomposites, 296-301. Peter which include starch, polyurethane and polyester nanocomposites. The work aims Awards and prizes: to develop smart, functional biopolymer Halley 2009 UQ Award for Programs that Enhance systems for biomedical, drug delivery and Learning Group Leader high-value industrial applications. The group has successfully: • initiated three new projects investigating water resistant starch, texture-modified foods and PHA polymers from waste; • published a book discussing reactive processing; • published and patented work on biopolymers and degradable polymers; and • maintained strong links with relevant industries in the areas of biofluids and biopolymers. The group has research projects in: • novel texture-modified foods for aged care for increased nutrition; • water resistant starch polymers for smart food packaging applications; • PHA polymers from waste streams; • PHA/lignin composites from sugar cane; • rheology of TPU nanocomposites; and

• supercritical CO2 processing of starch nanocomposites.

52 Professor Mark Kendall ARC Future Fellow and Group Leader

Research: Targeting the skin for needle-free, minimally-invasive vaccine delivery and diagnostics for disease

Professor Mark Kendall’s research group • multi-photon microscopy for in vivo Fernando GJP, Chen X, Prow TW, Crichton focuses on physical methods for delivering imaging following delivery of drugs and ML, Fairmaid EJ, Roberts MS, Frazer IH, biomolecules and stimuli to key immune vaccines to skin; Brown LE, Kendall MAF. (2010) Potent response-inducing cells located in the skin; • MPM non-invasive imaging of biological Immunity to Low Doses of Influenza Vaccine and extracting important biomolecules for interactions following drug delivery with by Probabilistic Guided Micro-Targeted Skin diagnostics purposes. micro-nanoprojection patches; Delivery in a Mouse Model. PLoS ONE 5(4), e10266. doi:10.1371/journal.pone.0010266. The ultimate goal of the research is to • measurement of mechanical properties in dramatically improve the cost and efficiency skin at the cellular and subcellular scale; Crichton ML, Ansaldo A, Chen X, Prow of vaccination and treatment of major and TW, Fernando GJP, Kendall MAF. (2010) diseases, such as malaria and influenza. To The effect of strain rate on the precision • developing medical devices for clinical achieve this goal, the group is: of penetration of short densely-packed use. microprojection array patches coated with • developing needle-free gene and drug The group has started a joint partnership vaccine. Biomaterials 31(16), 4562-4572. delivery and extraction technologies to research and development project with an and from the skin; international large vaccine company. Awards and prizes: • onvestigating micro-nanoprojection array 2009 Future Summit Australian Leadership patch (Nanopatch) technology; Key publications for the past five years: Award • measuring the key biological and Corrie S, Fernando GJP, Crichton ML, Inaurgural ARC Future Fellow, Professorial mechanical properties of skin; and Brunck MEG, Anderson CP, Kendall MAF. level • assessing clinical application. (2010) Surface-modified microprojection arrays for intradermal biomarker capture, 2010 Translational Research Excellence This multidisciplinary research spans with low non-specific protein binding. Lab Commercialisation Award from MERCK, in biomedical engineering (fluid mechanics; on a Chip 10, 2655-2658. conjunction with the Queensland Clinical micro-nanofabrication; solid mechanics), Trials Network diagnostics (multi-photon microscopy) and Chen X, Corbett H, Yukiko SR, Raphael dermatology and vaccinology. A, Fernando GJP, Fairmaid, EJ, Prow 2010 finalist for Australian Museum TW, Brown LE, Kendall MAF. (2010) Eureka prize. Category: Research by an Professor Kendall’s group has research Site-selectively coated, densely-packed Interdisciplinary Team Nanopatch Influenza projects covering: microprojection array patches for targeted Vaccination Team • micro-nanoprojection patches for delivery of vaccines to skin. Advanced Enterprize Business Plan Competition’s minimally-invasive and targeted delivery Functional Materials 21(3), 464-473. People’s Choice Award 2010 of genes and drugs to skin cells; Prow TP, Chen X, Prow NA, Fernando GJP, 2010 UniQuest Trailblazer Award in Student • micro-nanoprojection patches for Tan CSE, Raphael AP, Chang D, Ruutu category for original, innovative early- targeted gene and drug delivery to the MP, Jenkins DWK, Pyke A, Crichton ML, stage research and entrepreneurial ideas skin and improved DNA vaccines; Raphaelli K, Goh LYH, Frazer IH, Roberts competition • micro-nanoprojection patches for MS, Gardner J, Khromykh AA, Suhrbier A, improved sampling in diagnosis of Hall RA, Kendall MAF. (2010) Nanopatch- disease; Targeted Skin Vaccination against West Nile Virus and Chikungunya Virus in Mice. Small 6(16), 1776-84. (front cover).

53 Professor Max Lu UQ deputy vice-chancellor (research) and AIBN Group Leader

Research: Functional nanomaterials

Professor Max Lu is the Foundation Li ZY, Zhu GS, Lu GQ, Qiu SL, Yao XD. Liu G, Yang HG, Wang XW, Cheng LN, Pan Director and current Research Director of (2010) Ammonia Borane Confined by a J, Lu GQ, Cheng HM. (2009) Visible Light

the Australian Research Council Centre of Metal-Organic Framework for Chemical Responsive Nitrogen Doped Anatase TiO2 Excellence for Functional Nanomaterials. He Hydrogen Storage: Enhancing Kinetics Sheets with Dominant {001} Facets Derived is also Deputy Vice-Chancellor (Research) at and Eliminating Ammonia. Journal of the from TiN. Journal of the American Chemical The University of Queensland. American Chemical Society 132(5), 1490- Society 131(36), 12868-12869. 1491. The research interests of Professor Lu Hulicova-Jurcakova D, Puziy AM, and his group lie in the synthesis and Liu G, Niu P, Sun CH, Smith SC, Chen Poddubnaya OI, Suarez-Garcia F, Tascon molecular engineering of nanomaterials, ZG, Lu GQ, Cheng HM. (2010) Unique JMD, Lu G Q. (2009) Highly Stable such as inorganic nanoparticles, carbons, Electronic Structure Induced High Performance of Supercapacitors from nanoporous materials and membranes. Photoreactivity of Sulfur-Doped Graphitic Phosphorus-Enriched Carbons. Journal C3N4. Journal of the American Chemical of the American Chemical Society 131(14), The team is developing many innovative Society 132(33), 11642-11648. 5026-5027. applications of these materials in the areas of clean energy and environmental Liu J, Qiao SZ, Hartono SB, Lu GQ. (2010) Zhang L, Qiao SZ, Jin YG, Chen ZG, Gu HC, technologies, and in biomedical fields. Monodisperse Yolk-Shell Nanoparticles with Lu GQ. (2008) Magnetic hollow spheres a Hierarchical Porous Structure for Delivery of periodic mesoporous organosilica and Specifically, Professor Lu’s group has Vehicles and Nanoreactors. Angewandte Fe3O4 nanocrystals: Fabrication and research projects in the areas of: Chemie-International Edition 49(29), 4981- structure control. Advanced Materials 20(4), • visible light photocatalysts for solar and 4985. 805-809. hydrogen energy generation and storage; Chen ZG, Cheng LN, Xu HY, Liu JZ, Zou J, Yang HG, Sun CH, Qiao SZ, Zou J, Liu • nanoparticles and mesoporous carriers Sekiguchi T, Lu GQ, Cheng HM. (2010) ZnS G, Smith SC, Cheng HM, Lu GQ. (2008) for drug and vaccine delivery; Branched Architectures as Optoelectronic Anatase TiO2 single crystals with a large • nanostructured materials for high-density Devices and Field Emitters. Advanced percentage of reactive facets. Nature supercapacitors and batteries; and Materials 22(21), 2376-2380. 453(7195), 638-641. • efficient catalysts and processes for Yang HG, Liu G, Qiao SZ, Sun CH, Jin YG, Wang DW, Li F, Liu M, Lu GQ, Cheng HM. renewable energy. Smith SC, Zou J, Cheng HM, Lu GQ. (2009) (2008) 3D aperiodic hierarchical porous Solvothermal Synthesis and Photoreactivity graphitic carbon material for high-rate Key publications for the past five years: of Anatase TiO2 Nanosheets with Dominant electrochemical capacitive energy storage. Li Z, Cheng LN, Sun Q, Zhu ZH, Riley MJ, {001} Facets. Journal of the American Angewandte Chemie-International Edition Aljada M, Cheng ZX, Wang XL, Hanson Chemical Society 131(11), 4078-4083. 47(2), 373-376. GR, Qiao SZ, Smith SC, Lu GQ. (2010) Wang LZ, Tang FQ, Ozawa K, Chen ZG, Diluted Magnetic Semiconductor Nanowires Awards and prizes: Mukherj A, Zhu YC, Zou J, Cheng HM, Lu Prepared by the Solution-Liquid-Solid GQ. (2009) A General Single-Source Route Aluminus of Year (Research), Queensland Method. Angewandte Chemie-International for the Preparation of Hollow Nanoporous Education International Award 2010 Edition 49(15), 2777-2781. Metal Oxide Structures. Angewandte Australia’s Top 100 Most Influential Chemie-International Edition 48(38), 7048- Engineers, 2010, Engineers Australia 7051. ISI Highly Cited Researcher in Materials Science, Thomson Reuters

54 Associate Professor Stephen Mahler

Research: Developing biological medicines

The principal theme of Associate Professor Matigian N, Abrahamsen G, Sutharsan Marcal H, Wanandy NS, Stephen Mahler’s research is discovering R, Cook A, Nouwens A, Bellette B, Vitale Sanguanchaipaiwong V, Woolnough CE, and developing biologic medicines. A, An J, Anderson M, Beckhouse B, Cecil Lauto A, Mahler SM, Foster LJR. (2008) R, Chalk A. Cochrane J, Fan Y, Féron BioPEGylation of polyhydroxyalkanoates: His research activities include a balanced F, McCurdy R, McGrath J, Perry C, Raju Influence on properties and satellite stem mix of basic and applied research. As a J, Ravishankar S, Silburn P, Sutherland cell cycle. Biomacromolecules 9, 2719– biotechnologist, Associate Professor Mahler G, Mahler SM, Mellick G, Wood S, Sue C, 2726. has specialised in applied immunology, Wells C, Mackay-Sim A. (2010) Disease- proteomics and bioengineering. Vari F, Munster DJ, Hsu JL, Rossetti TR, specific, neurosphere-derived cells as Mahler SM, Gray PP, Turtle, CJ, Prue RL, A highlight of his present research includes models for brain disorders. Disease Models Hart DNJ. (2008) Practical blood dendritic isolating monoclonal antibodies from large and Mechanisms 3, 785-798. cell vaccination for immunotherapy of immunoglobulin gene libraries using high De Leon EJ, Yuan FF, Pearson H, Marquis multiple myeloma. British J Haematology throughput selection techniques against C, Mahler SM. (2009) Evidence of 143, 374-377. selected targets. The targets are associated heterogeneity in the antibody response with cancer, infectious disease and graft Dinnes DM, Marcal H, Raftery M, Labow against the platelet antigen 3a; recognition versus host disease. RS, Mahler SM. (2008) The macrophage- of an 11-mer peptide carrying the HPA-3a biomaterial interface: A proteomic analysis Another highlight is isolating antibody polymorphic determinant. Vox Sanguinis 96, of the conditioned medium. J Chem Technol lead that binds to dendritic cells. The 252-255, and Biotechnol. 83, 482-495. research is part of a collaboration with Wang XS, Shao B, Heinecke J, Mahler SM, the Mater Medical Research Institute, Dinnes DM, Marcal H, Santerre JP, Stocker R. (2009) Detection of methionine and is associated with a novel approach Mahler SM, Labow RS. (2007) Material sulfoxide-containing, oxidized apolipoprotein for preventing graft versus host disease, surfacesaffect the protein expression AI in HDL and plasma by ELISA. J Lipid through antibody-mediated depletion of patterns of human macrophages: a Research 50, 586-594. dendritic cells. The lead antibody has proteomic approach. Journal of Biomedical shown to be effective in in vitro and Materials Research 80, 895-908. in vivo bioassays, and is undergoing further development for potential future clinical trial.

Key publications for the past five years: Falconer RJ, Jackson-Matthews D, Mahler SM. (2011) Analytical strategies for assessing comparability of biosimilars. J Chem Technol and Biotechnol. (Accepted for publication March 2011). Mahler SM. (2011) Biologics and biosimilars: emerging technologies driving global opportunity. J Chem Technol and Biotechnol. (Accepted for publication March 2011). Jones ML, Seldon T, Smede M, Linville A, Chin DY, Barnard R, Mahler SM, Munster D, Hart D, Gray PP, Munro TP. (2010) A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies. Journal of Immunological Methods 354, 85-90.

55 Associate Professor Darren Martin Group Leader

Research: Polymer nanocomposites and nanotoxicology

Associate Professor Darren Martin’s primary consumer products, or medical device Key publications for the past five years: research themes are the processing and component applications. For example, his Rowlands AS, Lim SA, Martin D, Cooper- structure-property performance of novel research group is working on new flexible White JJ. (2007) Polyurethane/poly(lactic- polymeric biomaterials; renewable-based nanocomposite insulation materials for co-glycolic acid composite scaffolds polymers and nanocomposites; and the Cochlear implants and high-pressure tubing fabricated by thermally induced phase toxicology of engineered nanoparticles. and hoses. separation. Biomaterials 28, 2109-2121. These interests overlap significantly, Associate Professor Martin is the chief with mature initiatives now well under Ladewig BP, Knott RB, Hill AJ, Riches JD, scientific officer of start-up company way, investigating the physical and White JW, Martin DJ, da Costa JCD, Lu TenasiTech, which is commercialising the biological performance of polyurethane GQ. (2007) Physical and electrochemical polyurethane nanocomposites. nanocomposites for biomedical and characterisation of nanocomposite industrial applications. The Martin research group has projects in: membranes of Nafion and functionalised silicon oxide. Chem Mater 19(9), 2372-2381. There is also parallel research that links • thermoplastic polyurethane a better understanding of the molecular nanocomposites, through TenasiTech Pty Cao Y, Zhang B, Croll TI, Rolfe BE, and cellular mechanisms associated with Ltd, Cochlear Ltd and Aortech Pty Ltd; Campbell JH, Campbell GR, Martin DJ, nanoparticle toxicology with measurements • the toxicology of engineered Cooper-White JJ. (2006) Engineering tissue of industrial exposure and bio-distribution. nanoparticles, through ARC and NHMRC tubes using novel multilayered scaffolds in One of Associate Professor Martin’s funded projects; and the rabbit and rat peritoneal cavity. Tissue Engineering 12(4), 1020-1021. key research aims is to shift the strong • renewable polymers and composites underlying science and engineering based on resin from spinifex native Musumeci AW, Schiller TL, Xu ZP, Minchin taking place in these projects towards grasses – an ARC funded project. RF, Martin DJ, Smith SV. (2010) Synthesis and characterization of dual radiolabeled layered double hydroxide nanoparticles for use in in vitro and in vivo nanotoxicology studies. Journal of Physical Chemistry C, 114(2), 734-740. McNally T, Pötschke P, Halley P, Murphy M, Martin DJ, Bell SEJ, Brennan GP, Bein D, Lemoine P, Quinn JP. (2005) Polyethylene multiwalled carbon nanotube composites. Polymer 46(19), 8222-8232. Musumeci AW, Gosztola D, Schiller TL, Dimitrijevic NM, Mujica V, Martin DJ. (2009) SERS of semiconducting nanoparticles (TiO2 hybrid composites). JACS Communication 131(17), 6040-6041. Smart S, Cassady A, Lu GQ, Martin DJ. (2006) The Biocompatibility of Carbon Nanotubes: A Review. Carbon 44, 1034- 1047.

Finnigan B, Jack K, Campbell K, Halley P, Truss R, Casey P, Cookson D, King S, Martin DJ. (2005) Segmented Polyurethane Nanocomposites: Impact of Controlled Size Nanofillers on the Morphological Response to Uniaxial Deformation. Macromolecules 38(17), 7386-7396.

56 Professor Anton Middelberg Smart Futures Premier’s Fellow and Group Leader

Research: Biomolecular engineering

Professor Anton Middelberg and his Professor Middelberg’s research brings Chuan YP, Fan YY, Lua LHL, Middelberg research team focuses on designing together knowledge from engineering and APJ. (2010) Virus assembly occurs and processing engineered proteins the physical and life sciences. following a pH- or Ca2+- triggered switch and peptides to develop new functional in the thermodynamic attraction between The group has research projects in: products and new manufacturing methods. structural protein capsomeres. J R Soc The work brings together bioengineering • protein and nanoparticle technology for Interface 7, 409-421. and nanotechnology, with application in new vaccines; He LZ, Wang H, Garamus VM, Hanley T, vaccines and biopharmaceuticals, and bio- • the aggregation of virus-like particle Lensch M, Gabius HJ, Fee CJ, Middelberg renewable sustainable materials. vaccines; APJ. (2010) Analysis of MonoPEGylated Professor Middelberg and his group • vaccine nano-emulsions; Human Galectin-2 by Small-Angle X-ray address global opportunities such as: • recovery and modification of and Neutron Scattering: Concentration Dependence of PEG Conformation in the • developing new vaccine technologies that biopharmaceuticals; Conjugate. Biomacromolecules 11(12), change the way we combat infectious • design and bioprocessing of sustainable 3504-3510. and chronic diseases, including influenza biosurfactants including pepfactants; and and arthritis; • nanomaterial manufacture through Ding T, Li RY, Zeitler JA, Huber TL, Gladden • understanding how biopharmaceuticals biomolecular templating. LF, Middelberg APJ, Falconer RJ. (2010) behave in solution and manufacturing Terahertz and far infrared spectroscopy processes, to devise new processes Key publications for the past five years: of alanine-rich peptides having variable ellipticity. Optics Express 18(26), 27431- to recover products from complex Liew MWO, Rajendran A, Middelberg APJ. 27444. suspensions; and (2010) Microbial production of virus-like • using bio-inspired approaches to deliver particle vaccine protein at gram-per-litre Awards and prizes: new manufactured materials, such as levels. J Biotechnol 150(2), 224-231. customised surfactants, self-assembling 2010 Queensland Smart Futures Premier’s Ding Y, Chuan YP, He L, Middelberg APJ. Fellow peptides and nanostructured materials, (2010) Modeling the competition between from sustainable resources. aggregation and self-assembly during virus- like particle processing. Biotechnol Bioeng 107(3), 550-560. (front cover).

57 Professor Michael Monteiro ARC Future Fellow and Group Leader

Research: Designer polymer nanoreactors for use in biomedical applications and environmentally friendly organic reactions

Professor Michael Monteiro’s research It is envisaged that this methodology will Skwarczynski M, Zaman M, Urbani CN, Lin focuses on synthesising complex polymer expand the range of structures available to IC, Jia Z, Batzloff MR, Good MF, Monteiro architectures. The tailor-made architectures materials scientists. MJ, Toth I. (2010) Polyacrylate dendrimer have the capacity to self-assemble into nanoparticles: a self-adjuvanting vaccine The group has projects in the areas of: nanostructures, such as rods, vesicles, delivery system. Angew Chem Int Ed Engl spheres or donuts. The nanostructure • nanoreactors for polymer and organic 49(33), 5742-5745. confers important characteristics that reactions in water; Percec V, Guliashvili T, Ladislaw JS, can be applied in drug delivery and tissue • customised nanostructures for drug and Wistrand A, Stjerndahl A, Sienkowska MJ, regeneration. vaccine delivery; and Monteiro MJ, Sahoo S. (2006) Ultrafast In one example, a new, self-adjuvanting • nanotoxicology of these designer Synthesis of Ultrahigh Molar Mass vaccine was created through the self- nanostructures. Polymers by Metal-Catalyzed Living Radical assembly of a dendrimer consisting of Polymerization of Acrylates, Methacrylates, epitopes covalently bound to a polymeric Key publications for the past five years: and Vinyl Chloride Mediated by SET at core. Incorporating certain polymers into Sebakhy KO, Kessel S, Monteiro MJ. 25°C. J Am Chem Soc 128(43), 14156- these structures is proving useful for siRNA (2010) Nanoreactors to Synthesize Well- 14165. delivery. Other work by Professor Monteiro defined Polymer Nanoparticles: Decoupling Whittaker MR, Urbani CN, Monteiro MJ. and his research group has demonstrated Particle Size from Molecular Weight. (2006) Synthesis of 3-Miktoarm Stars and that highly dense nanoparticles can Macromolecules 43(23), 9598-9600. 1st Generation Mikto Dendritic Copolymers denature specific serum proteins, which Deng ZJ, Liang MT, Monteiro MJ, Toth I, by “Living” Radical Polymerization and induces the activation of certain biochemical Minchin RF. (2011) Nanoparticle-induced “Click” Chemistry. J Am Chem Soc 128(35), pathways. unfolding of fibrinogen promotes Mac-1 11360-11361. Custom-made polymer architectures receptor activation and inflammation. Nature Lonsdale DE, Monteiro MJ. (2010) Various designed by Professor Monteiro’s group Nanotechnology 6(1), 39-44. polystyrene topologies built from tailored have also been used as nanoreactors. The Monteiro MJ. (2010) Nanoreactors for cyclic polystyrene via CuAAC reactions. group has developed a completely new way Polymerizations and Organic Reactions. Chem Commun (Cambridge, UK) 46(42), of conducting polymerisations in water by Macromolecules 43(3), 1159-1168. 7945-7947. creating the desired nanoenvironment using custom-made nanoreactors. Liang M, Lin IC, Whittaker MR, Minchin RF, Monteiro MJ, Toth I. (2010) Cellular Uptake of This new water-based methodology has Densely Packed Polymer Coatings on Gold been driven by increased demand for Nanoparticles. ACS Nano 4(1), 403-413. environmentally-friendly and economically- competitive polymeric materials for use in Kulis J, Bell CA, Micallef AS, Jia ZF, the coatings, biomedical and electronic Monteiro MJ. (2010) Rapid, Selective, industries. and Reversible Nitroxide Radical Coupling (NRC) Reactions at Ambient Temperature. Macromolecules 42(21), 8218-8227.

58 Professor Lars Nielsen Group Leader

Research: Systems and synthetic biology

Systems biology provides the means Key publications for the past five years: of answering intricate questions about Petrasovits LA, Purnell MP, Nielsen complex biological systems. The questions LK, Brumbley SM. (2007) Production of considered by Professor Lars Nielsen’s polyhydroxybutyrate in sugarcane. Plant research group are very diverse, but Biotechnology Journal 5, 162-172. generally inspired by some practical applications. For example: Quek L-E, Nielsen LK. (2008) On the reconstruction of the Mus musculus How do we efficiently convert stem cells genome-scale metabolic network model. into white and red blood cells for use in anti- Genome Informatics 21, 89-100. bacterial treatment? Chen W, Marcellin E, Hung J, Nielsen LK. Why does transgenic sugarcane produce (2009) Hyaluronan molecular weight is plastic efficiently in one type of leaf cells, but controlled by UDP-N- acetylglucosamine not the other? concentration in Streptococcus How do we efficiently engineer E. coli and zooepidemicus. J Biol Chem 284, 18007- yeast to produce fuels and chemicals from 18014. sucrose? Timmins NE, Palfreyman E, Marturana F, Given the number and diversity of mutations Dietmair S, Luikenga S, Lopez G, Fung YL, in any given tumour type, what are the Minchinton R, Nielsen LK. (2009) Clinical common signalling phenotypes responsible Scale Ex vivo Manufacture of Neutrophils for malignancy? from Hematopoietic Progenitor Cells. The common challenge for all these Biotechnol Bioeng 104, 832-840. problems lies in formulating the question de Oliveira Dal’molin CG, Quek LE, in such a way that it will yield a meaningful Palfreyman RW, Brumbley SM, Nielsen answer when using the appropriate LK. (2010) AraGEM - a Genome-Scale mathematical, statistical and analytical tools. Reconstruction of the Primary Metabolic A key focus of Professor Nielsen’s group is Network in Arabidopsis thaliana. Plant to develop the engineering frameworks and Physiol 152, 579–589. tools required to meet this challenge. With an applied focus, the proof is ultimately that the understanding gained can be used to synthesise better systems and processes. In addition to various in-house model systems, Professor Nielsen’s group works closely with Australian and international companies and academic groups exploring the potential of the novel strategies developed. The group has research projects in the areas of: • developing biological replacements for materials currently produced from petrochemical feedstocks; • producing plastics in sugar cane; • expanding neutrophils from stem cells for therapeutic purposes; • modelling and analysis of mammalian cell metabolism using genome-scale models and metabolomics; and • modelling signalling and transcription regulation networks in animal cells.

59 Dr Steven Reid Group Leader

Research: Systems biology approach to the production of baculovirus biopesticides

Specific research projects include: • development of a Heliothis bacmid system for manipulation of the H.arm virus genome (gene knockouts). This system will be used to generate altered viruses for further transciptomic and metabolomic studies; • using real time PCR to quantify virus- binding kinetics to enable optimisation of early process steps for manufacturing the virus in vitro; and • developing sample extraction procedures and appropriate HPLC/GC-MS techniques for quantifying intracellular metabolite levels for infected and non- infected cells in culture, which will enable the metabolomic studies.

Key publications in the past five years: Chan L, Reid S, Nielsen LK. (2010) The kinetics of virus production from cell culture. In Wiley Encyclopedia of Industrial Biotechnology (Flickinger MC, Ed.), pp. 1-24, Hoboken, NJ, US. Pedrini MRS, Chan LCL, Nielsen LK, Dr Steven Reid’s research group has Reid S. (2006) In vitro production of a process patent on a procedure for Helicoverpa armigera single-nucleocapsid producing baculoviruses using fermentation. nucleopolyhedrovirus. Brazilian Archives of The lead product is a baculovirus targeting Biology and Technology 49 (special issue), the Helicoverpa pest species, the 35-41. subjugation of which is responsible for a Pedrini MRS, Christian P, Nielsen LK, $US3.2 billion annual market in traditional Reid S, Chan LCL. (2006) Importance chemical pesticides. of virus-medium interactions on the A baculovirus product manufactured by Dr biological activity of wild-type Heliothine Reid’s group and formulated by Bioflexus nucleopolyhedroviruses propagated via has been registered for use on Australian suspension insect cell cultures. J Virol crops to combat heliothis caterpillars (more Methods 136, 267-272. widely known as the cotton bollworm), Pedrini MRS, Nielsen LK, Reid S, Chan LCL. under the trade name of Heliocide. Dr Reid’s (2005) Properties of a Unique Mutant of group is undertaking further research to Helicoverpa armigera Single – Nucleocapsid increase current fermentation yields, which Nucleopolyhedrovirus that Exhibits a will enable the product’s manufacture and Partial Many Polyhedra and Few Polyhedra evaluation in the niche Australian market. Phenotype on Extended Serial Passaging in The group is collaborating with AIBN’s Suspension Cell Cultures. In Vitro Cell Dev Professor Lars Nielsen to use a systems Biol – Animal 41, 289-297. biology approach using transcriptomic and metabolomic techniques in an effort to understand how the virus interacts with host cells in culture. The group anticipates further increases in yield, making it cost effective in broader markets, both nationally and internationally.

60 Research: Computational molecular science

The research of Professor Sean Smith • simulation of biological dendrimer-DNA Du AJ, Chen Y, Zhu ZH, Lu GQ, Smith SC. and his group applies theoretical and interactions for gene delivery applications; (2009) C-BN Single Walled Nanotubes from computational studies at the molecular • computational studies of C and BN Hybrid Connection of BN/C Nanoribbons: scale to gain enabling insights that nanotube and nanoribbon reactivity and Prediction by Ab Initio Density Functional will facilitate progress towards new functionalisation; Calculations. J Amer Chem Soc 131, 1682- technologies in electronics, sustainable 1683. • quantum dynamical studies of H transport energy and biomolecular/biomedical in confined systems; and Yao XD, Lu GQ, Li L, Sun CH, Du AJ, Smith applications. His research group conducts SC, Zhu ZH. (2009) Lithium-Catalyzed computational explorations of: • computational kinetics and quantum Dehydrogenation of Ammonia Borane dynamical studies of elementary reactions • chemical kinetics; within Mesoporous Carbon Framework for for combustion and atmospheric • catalysis; and Chemical Hydrogen Storage. Advanced chemistry. • complexation/association phenomena Functional Materials 19, 265-271. and transport within: Key publications for the past five years: Yang HG, Sun CH, Qiao SZ, Zhou J, Smith – nanostructured materials; Du AJ, Zhu ZH, Smith SC. (2010) SC, Cheng HM, Lu GQ. (2008) Anatase – proteins; Multifunctional Porous Graphene for TiO2 single crystals with a large percentage – hybrid nano-bio systems; and Nanoelectronics and Hydrogen Storage: of {001} facets. Nature 453, 638-641. – gaseous environments. New Properties Revealed by First Principle Du AJ, Smith SC, Lu GQ. (2007) Formation The research is performed at the atomistic Calculations. J Amer Chem Soc 132(9), of Single Walled Carbon Nanotube Via the level, including advanced applications 2876-2877. Interaction of Graphene Nanoribbons: ab of solid state and molecular electronic Li Z, Cheng L, Sun Q, Zhu Z, Riley MJ, initio Density Functional Calculations. Nano structure theory, molecular dynamics, Aljada M, Cheng Z, Wang X, Qiao S, Smith Lett 7, 3349-3354. quantum dynamics and kinetics theories. SC, Lu GQ. (2010) Diluted Magnetic Yao X, Wu CZ, Du AJ, Zou J, He Y, Zhu The studies are conducted in an Semiconductor Nanowires Prepared by ZH, Wang P, Cheng HM, Smith SC, Lu interdisciplinary context, in close Solution-Liquid-Solid Method. Angew Chem GQ. (2007) Metallic and Carbon Nanotube- collaboration with experimental and other Int Ed 49(15), 2777-2781. Catalyzed Coupling of Hydrogenation theoretical groups. They require state- Du AJ, Chen Y, Zhu ZH, Amal R, Lu GQ, in Magnesium. J Amer Chem Soc 129, of-the-art high-performance computing Smith SC. (2009) Dots versus Antidots: 15650-15654. facilities for their implementation. Computational Exploration of Structure, Du AJ, Smith SC, Yao XD, Lu G. (2007) Professor Smith’s group has research Magnetism and Half-metallicity in Boron- Hydrogen Spillover Mechanism on a Pd- projects in: Nitride Nanostructures. J Amer Chem Soc doped Mg Surface as Revealed by ab initio • computational studies of light metal 131, 17354-17359. Density Functional Calculations. J Amer hydride nanocomposite materials for Yang HG, Liu G, Qiao SZ, Sun CH, Jin YG, Chem Soc 129, 10201-10204. hydrogen storage; Smith SC, Zou J, Cheng HM, Lu GQ. (2009) • fluorescent proteins: photophysics, Solvothermal Synthesis and Photoreactivity

mechanism and dynamics; of Anatase TiO2 Nanosheets with Dominant • simulation of layered-double-hydroxide {001} Facets. J Amer Chem Soc 131, 4078- nanoparticle-DNA interactions for gene 4083. delivery applications;

Professor Sean Smith Group Leader

61 Professor Mat t Trau Group Leader

Key publications in the past 12 months: Connolly AR, Trau M. (2011) Rapid DNA detection by Beacon Assisted Detection Amplification. Nature Protocols. (Accepted Feb 2011) In print. Palanisamy R, Connolly AR, Trau M. (2011) Epiallele quantification using molecular inversion probes. Analytical Chemistry 83(7), 2631-2637. Corrie SR, Sova P, Feng Q, Blair T, Kiviat NB, Trau M. (2011) Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle- based flow cytometry assay. Analyst 136, 688-691. (front cover/feature article). Connolly A, Trau M. (2010) Isothermal Detection of DNA by Beacon Assisted Detection Amplification (BAD AMP). Angewandte Chemie (International Ed.) 122, 2780-2782. Roberts GS, Kozak D, Anderson W, Broom MF, Vogel R, Trau M. (2010) Tunable Nano/ Micropores for Particle Detection and Discrimination: Scanning Ion Occlusion Spectroscopy. Small 6(23), 2653-2658.

Research: Nanotechnology, biomarkers and Patents: molecular diagnostics Trau, Johnston. Synthesis and use of organosilica particles (US7754646-B2). Granted July 13, 2010. The use of biomarkers (molecules that The development of nanoscaled biosensors indicate the onset and status of disease) is highly multidisciplinary, bringing Battersby, Bryant, Trau. Carriers for is emerging as one of the most promising together scientists from many areas, such combinatorial compound libraries strategies for disease management. In as nanotechnology, molecular biology, (CA2352082-C). Granted September 21, nearly all forms of cancer, early diagnosis biochemistry, pathology, medicine and 2010. can lead to a cure at a fraction of the cost of bioinformatics. The research involves strong currently ineffective treatments for late-stage interactions with the Peter MacCallum Awards and prizes: disease. It is predicted the development of Cancer Centre; the Garvan Institute; The National Breast Cancer Foundation new biomarker diagnostic technologies will the University of Newcastle; the Fred awarded Professor Trau the 2010 Pink simultaneously improve survival rates and Hutchinson Cancer Research Center; the Circle Breast Cancer Research Award. quality of life, while significantly reducing Seattle Biomedical Research Institute; the health care costs. Benaroya Research Institute, the University of Washington; and Nanomics BioSystems At AIBN’s Centre for Biomarker Research Pty Ltd. and Development, Professor Matt Trau and his research group are currently focused on The single molecule readout area of several projects: research focuses on understanding tunable elastic nanopore sensors that are being • nano-scaled biosensors for epigenetic developed with New Zealand’s Izon Science readouts in breast cancer; Ltd. The sensors are used for monitoring • novel nano-devices for protein capture in particle-particle and particle-biomolecule diagnostics; interactions, and quantitative sizing of • single molecule readouts within elastic colloidal dispersions. nanopores; and • nanotechnology devices for capturing circulating tumour cells.

62 Associate Professor Christine Wells NHMRC Career Development Award and Group Leader

Research: Cellular differentiation and activation

Associate Professor Christine Wells was databases, but could bring important • genomics, bioinformatics and cell biology appointed to AIBN in 2010 and took up information on stem cell behaviours if to investigate the transcriptional analysis her position in January 2011. She heads collated and interrogated in a systematic of monocytes infected with an exemplar a research group that uses genomic manner. The Stemformatics web portal is pathogen series; technologies to understand genetic and aimed at collating the data and enabling • genomic and cell biology to understand environmental factors that lead to disease stem cell researchers to investigate the the epigenetic events that modify immune susceptibility. The group is interested in: gene signatures that correlate with stem cell differentiation; and cell function. The project is funded through • innate immune function and susceptibility • developing new computational approaches the Australian Stem Cell Centre as a to infectious disease; and for predictive models of cell fate. collaboration between the Wells group at • stem cell differentiation. AIBN; the Hilton group at the Walter and Key publications for the past five years: The innate immune system provides the first Eliza Hall Institute; and the Grimmond group Mar J, Wells CA, Quackenbush J. (2011) line of defence against infection. Infectious at The University of Queensland’s Institute Defining an Informativeness Metric diseases remain the leading cause of death for Molecular Bioscience. world-wide. Many infectious agents have for Clustering Gene Expression Data. Stemformatics.org currently: been implicated in the onset of cancers, Bioinformatics. Feb 16 [Epub ahead of print]. tissue death and organ failure; and in some • hosts expertly curated public gene FANTOM4 consortium including Wells CA. cases are responsible for exacerbating expression data from exemplar stem cell (2009) The transcriptional network that chronic diseases. To mount effective datasets; controls growth arrest and differentiation in preventative programs, such as vaccinations, • facilitates simple gene searches across a human myeloid leukemia cell line. Nature or provide appropriate diagnosis and point mouse and human stem cell datasets; Genetics 41(5), 553-562 of care, researchers need to understand why • presents data in high-quality images; and some people are susceptible to infection, Wells CA, Salvage-Jones J, Li X, Hitchens whereas others can resolve infection without • provides transparent links to the original K, Butcher S, Murray R, Beckhouse A, medical intervention. source and facilitates access to the Lo Y, Cobbold C, Ma B, Orr S, Stewart L, primary data. Lebus D, Sobieszczuk P, Hume D, Stow, The Wells group’s research is aimed at: J, Blanchard, H, Ashman, B. (2008) The The Wells group is multidisciplinary, with • identifying the networks of genes macrophage inducible c-type lectin, Mincle, marshalled to fight an infection; expertise in cell biology and signalling, genomics, transcriptomics and bioinformatics. is an essential component of the innate • modelling the interactions of the immune It has collaborations with microbiology labs, immune response to Candida albicans. system with our environment; and clinicians and immunology groups through the Journal of Immunology 180(11), 7404-7413. • developing markers for improved diagnosis Australian Infectious Disease Research Centre FANTOM3 consortium including Wells and treatment of susceptible individuals. at UQ; and is a participant in the international CA. (2006) Genome-wide analysis of FANTOM (Functional Annotation of the The group’s most recent success has been mammalian promoter architecture and Mammalian Genome) and the Functional identifying the C-type Lectin Mincle, which has evolution. Nature Genetics 38(6), 626-635. Glycomics consortia. The group works closely a critical role in immune responses to fungal Wells CA, Chalk AM, Forrest A, Taylor D, with John Quackenbush and his group at diseases, including thrush, and has been Waddell N, Schroder K, Himes SR, Faulkner the Dana-Farber Cancer Institute; and the implicated in mycobacterial infections, such as G, Lo S, Kasukawa T et al. (2006) Alternate biostatistics group at the Harvard School of TB. The group is characterising the regulation transcription of the Toll-like receptor signalling Public Health, Boston. and function of Mincle in human health. cascade. Genome Biology 7(2), R10. Stem cell biology promises new strategies The group has research projects in: for regenerative medicine. The Wells group • genome biology to identify key genes Awards and prizes: recognises there is a wealth of stem cell determining macrophage differentiation 2010 Women in Technology Research data that is effectively hidden in public and activation; Excellence Award

63 Professor Andrew Wh it taker ARC Professorial Fellow and Group Leader

Research: Polymer chemistry

Professor Andrew Whittaker and his team Key publications for the past five years: Peng H, Xiao Y, Mao X, Chen L, Crawford are working to develop novel polymeric Whittaker AK. (2006) The Structure of R, Whittaker AK. (2008) Amphiphilic Triblock materials for application in: Polymer Networks. Handbook of Modern Copolymers of Methoxy-poly(ethylene • biomaterials for diagnosis and treatment Magnetic Resonance 1, 579-585. G. Webb glycol)-b-poly(L-lactide)-b-poly(L-lysine) for of disease; (Ed.), Springer, Dordrecht, Neth. Enhancement of Osteoblast Attachment and Growth. Biomacromolecules 10(1), 95-104. • photolithography for manufacturing Zainuddin, Hill DJT, Traian CV, Whittaker AK, integrated circuits; and Kemp A. (2006) Experimental Calcification Peng H, Blakey I, Dargaville B, Rasoul F, • molecular imaging agents for disease of hydroxyethyl methacrylate-Based Rose S, Whittaker AK. (2009) Synthesis identification using nuclear magnetic Hydrogels in the Presence of Albumin and and Evaluation of Partly Fluorinated Block resonance imaging. a Comparison to the in Vivo Calcification. Copolymers as MRI Imaging Agents. Biomacromolecules 10(2), 374-381. This research is underpinned by extensive Biomacromolecules 7(6), 1758-1765. expertise in polymer synthetic chemistry, Thurecht KJ, Hill DJT, Whittaker A K. (2006) Mao X, Peng H, Ling J, Friis T, Whittaker polymer physical chemistry, interactions NMR microscopy: a tool for measuring AK, Crawford R, Xiao Y. (2009) Enhanced with biological systems and magnetic human bone marrow stromal cell affinity monomer diffusion in supercritical CO2. resonance technology. Macromolecular Chemistry and Physics for modified poly(-lactide) surfaces by the The group’s biomaterials research is 207(17), 1539-1545. upregulation of adhesion molecular genes. Biomaterials 30(36), 6903-6911. targeting various applications including: Plummer R, Hill DJT, Whittaker AK. • polymers for use as tissue implants; (2006) Solution Properties of Star and Thurecht KJ, Blakey I, Peng H, Squires O, • improving drug delivery; and Linear Poly(N-isopropylacrylamide). Hsu S, Alexander C, Whittaker AK. (2010) • aiding medical diagnosis. Macromolecules 39(24), 8379-8388. Functional Hyperbranched Polymers: Toward Targeted in Vivo 19F Magnetic This is in addition to projects shedding Tan I, Flanagan BM, Halley PJ, Whittaker AK, Resonance Imaging Using Designed light on drug delivery, the fundamentals Gidley MJ. (2007) A Method for Estimating Macromolecules. Journal of the American of hydrogel polymer networks, surface the Nature and Relative Proportions of Chemical Society 132(15), 5336-5337. modification and biosensors. Amorphous, Single, and Double-Helical Components in Starch Granules by 13C CP/ Blakey I, Thurecht KJ, Whittaker AK. (2010) Using immersion and ultra-violet immersion MAS NMR. Biomacromolecules 8(3), 885-891. lithography, the group is also investigating the High-pressure real-time 129Xe NMR: challenges facing the microelectronics industry Blakey I, George GA, Hill DJT, Liu monitoring of surfactant conformation as it struggles to incorporate nanometre-sized H, Rasoul F, Rintoul L, Zimmerman during the self-assembly of reverse micelles features on integrated circuits. P, Whittaker AK. (2007) Mechanism of in supercritical carbon dioxide. Chemical 157 nm Photodegradation of Poly[4,5- Communications (Cambridge, UK) 46(16), Research projects include: difluoro-2,2- bis(trifluoromethyl)-1,3- 2850-2852. • polymers for 193 nm immersion lithography; dioxole-co-tetrafluoroethylene] (Teflon AF). Ngo TTV, Duchet-Rumeau J, Whittaker • polymers for EUV lithography; Macromolecules 40(25), 8954-8961. • block copolymers for healing of line edge AK, Gerard, JF. (2010) Processing of roughness; Zainuddin, Le TT, Park Y, Chirila TV, Halley nanocomposite foams in supercritical • polymers for artificial vitreous; PJ, Whittaker AK. (2008) The behaviour of carbon dioxide. Part I: Effect of surfactant. • artificial blood vessels; aged regenerated Bombyx mori silk fibroin Polymer 51(15), 3436-3444. 1 • dental bone repair; solutions studied by H NMR and rheology. Paterson SM, Brown DH, Chirila TV, Keen • molecular imaging agents; Biomaterials 29(32), 4268-4274. I, Whittaker AK, Baker MV. (2010) The • hybrid imaging agents; Kealley CS, Rout MK, Dezfouli MR, synthesis of water-soluble PHEMA via • ultrasound contrast agents; Strounina E, Whittaker AK, Appelqvist ARGET ATRP in protic media. Journal • diffusion in hydrogels; IAM, Lillford PJ, Gilbert EP, Gidley of Polymer Science, Part A: Polymer • novel hydrogel networks; and MJ. (2008) Structure and Molecular Chemistry 48(18), 4084-4092. • spinal cord repair. Mobility of Soy Glycinin in the Solid State. Biomacromolecules 9(10), 2937-2946.

64 Associate Professor Ernst Wolvetang Group Leader

Research: Human pluripotent stem cells for regenerative medicine

Associate Professor Ernst Wolvetang’s Key publications in the past five years: Hannan N, Jamshidi P, Pera MF, Wolvetang research is focused on developing human Wolvetang EJ, Herszfeld D, Langton-Bunker EJ. (2009) BMP -11 and Myostatin Maintain pluripotent stem cell-based therapies and E, Chung T, Filipczyk A, Houssami S, Koh Human Embryonic Stem Cells in Feeder disease models. K, Laslett AL, Michalska A, Nguyen L, Free Cultures. Cloning and Stem Cells 11(3), Because human pluripotent stem cells can Reubinoff BE, Tellis I, Auerbach JM, Ording 427-435. be cultured indefinitely and can generate CJ, Looijenga LHJ, Pera MF. (2006) CD30 Doran MR, Frith JE, Prowse ABJ, Fitzpatrick every cell type of the human body, they are is a survival factor and a biomarker for J, Wolvetang EJ, Munro T, Gray PP, Cooper- transformed human pluripotent stem cells. the cell type of choice for stem cell based White JJ. (2010) Defined high protein Nature Biotech 24(3), 351-357. regenerative medicine, and discovery content surfaces for stem cell culture. platform for understanding the molecular Chung TL, Brena RM, Kolle G, Grimmond SM, Biomaterials 31(19), 5137-5142. basis of human disease and development. Berman BP, Laird PW, Pera MF, Wolvetang Hudson JE, Mills RJ, Frith JE, Brooke EJ. (2010) Vitamin C promotes widespread The ability to reprogram adult cells into G, Jaramillo-Ferrada P, Wolvetang EJ, pluripotent cells (called induced pluripotent yet specific demethylation of the hESC epigenome. Stem Cells 28(10), 1848-1855. Cooper-White JJ. (2010) A Defined Media stem cells (iPS cells)) that are essentially and Substrate for Expansion of Human equivalent to embryonic stem cells has Chung TL, Turner J, Thakar N, Kolle G, Cooper- Mesenchymal Stromal Cell Progenitors that removed the ethical concerns attached to White JJ, Grimmond SM, Pera MF, Wolvetang Enriches for Osteo-genic and Chondro-genic this brand of stem cell research. It also allows EJ. (2010) Ascorbate Promotes Epigenetic Precursors. Stem Cells Dev 20(1), 77-87. the generation of patient specific stem cells activation of CD30 in Human Embryonic that will not suffer from rejection. Generating Stem Cells. Stem Cells 28(10), 1782-1793. Prowse ABJ, Wilson J, Osborne GW, Gray iPS cells allows the creation of unique disease PP, Wolvetang EJ. (2009) Multiplexed Prowse A, Doran M, Cooper-White JJ, models previously not available to researchers. staining of live human embryonic stem cells Chong F, Munro T, Fitzpatrick J, Chung TL, for flow cytometric analysis of pluripotency Associate Professor Wolvetang joined AIBN Haylock DN, Gray PP, Wolvetang EJ. (2010) markers. Stem Cells and Development 18(8), in 2008. Current research activities in the Long-term culture of human embryonic stem Wolvetang group concentrate on: cells on recombinant vitronectin in ascorbate 1135-1140. • elucidating the role of specific signalling free media. Biomaterials 31(32), 8281-8288. Chung TL, Thaker N, Wolvetang EJ. (2010) pathways and the microenvironment Grandela C, Pera MF, Wolvetang EJ. (2007) Genetic and epigenetic instability of human in controlling the behaviour of human p53 is required for etoposide-induced pluripotent stem cells. The Open Stem Cell pluripotent cells to enable safer, more apoptosis of human embryonic stem cells. Journal (in press). efficient stem cell expansion and Stem Cell Research 1(2), 116-128. differentiation; and Vitale AM, Wolvetang EJ, Mackay-Sim Hannan NRF, Wolvetang EJ. (2008) Adipocyte A. (2011) Induced pluripotent cells: a new • generating species and patient-specific iPS differentiation in human embryonic stem cells technology to study human diseases. The cells to understand the molecular basis of transduced with Oct4 shRNA lentivirus. Stem International Journal of Biochemistry and disease and enable cellular therapy. Cells and Development 18(4), 653-660. Cell Biology (accepted for publication). Specific projects in these areas include: • developing novel cell reprogramming technologies; • generating Down Syndrome iPS cells to understand Alzheimer’s disease; • understanding the epigenetic effects of culture conditions; • elucidating BMP-SMAD signalling in human stem cells; • metabolomic analysis of human embryonic stem cells; and • Developing smart surfaces for stem cell expansion and differentiation. By combining cutting-edge molecular analysis and cell biology tools, the group will gain an in-depth understanding of the molecular machinery controlling human pluripotent stem cells and consequently be able to unlock the potential of these cells for application in regenerative medicine and drug development.

65 Professor Chengzhong (Michael) Yu ARC Future Fellow and Group Leader

Research: Nanomaterials for biotechnology, energy and environmental protection

Professor Chengzhong (Michael) Yu and Key publications for the past five years: his research group have an excellent Yang J, Zhou L, Zhao LZ, Zhang HW, Yin track record of research and innovation JN, Wei GF, Qian K, Wang YH, Yu CZ. in nanoporous and nano-materials with (2011) A Designed Nanoporous Material for various compositions, adjustable structures Phosphate Removal with High Efficiency. J and tailored functions for biotechnology, Mater Chem 21(8), 2489 - 2494. clean energy and environment protection. His group also focuses on investigating Zhu J, Tang JW, Zhao LZ, Zhou XF, Wang cancer carcinogenesis to identify YH, Yu CZ. (2010) Ultrasmall, Well- potential therapeutic targets and using Dispersed, Hollow Siliceous Spheres with nanotechnology to develop novel cancer Enhanced Endocytosis Properties. Small diagnosis and therapies. 6(2), 276-282. Research projects in the group include: Zhou L, Yang LC, Yuan P, Zou J, Wu YP, Yu CZ. (2010) Alpha-MoO Nanobelts: A High • a robust nanomaterial platform for 3 Performance Cathode Material for Lithium advanced delivery; Ion Batteries. J Phys Chem C 114, 21868- • nanoporous materials for bio-catalysis, 21872. bio-separation and bio-analysis; Qian K, Wan JJ, Huang XD, Yang PY, Liu • biomaterials for bone repair and dental BH, Yu CZ. (2010) A Smart Glycol-Directed applications; Nanodevice by Rationally Designed • skin cancer carcingenesis (EMT model), Macroporous Materials. Chem Eur J 16, development and therapy; and 822-828. • advanced nanomaterials for sustainable Yuan P, Liu N, Zhao LZ, Zhou XF, Zhou L, environment and energy applications. Auchterlonie GJ, Yao XD, Drennan J, Lu GQ, Zou J, Yu CZ. (2008) Solving Complex Concentric Circular Mesostructures Using Electron Tomography. Angew Chem-Int Edit 47(35), 6670.

66 Grants

Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

Australian ARC Discovery Prof Anton Sustainable processes for next- 2010-2012 $160,000 Competitive Projects Middelberg generation surface coatings and Grant Income core-shell nanoparticles based on biomolecular templating

Australian ARC Discovery Prof Mark Prof Ian Frazer, Improving immune response to 2010-2012 $270,000 Competitive Projects Kendall Prof Michael Roberts, vaccines by selective targeting of Grant Income Prof Davide Ambrosi epithelial regions with the Nanopatch Australian ARC Discovery Dr Jian Liu Nanostructured degradable polymer 2010-2012 $80,182 Competitive Projects for drug delivery Grant Income Australian ARC Discovery A/Prof Idriss Dr Kristofer Thurecht, Multimodal biomedical imaging probes: 2010-2012 $90,000 Competitive Projects Blakey Peter Fredericks, Cameron development of advanced polymer Grant Income Alexander nanocomposite devices for oncology Australian ARC Discovery Prof Justin Prof Nicholas Fisk, Scalable, high throughput microfluidic 2010-2012 $130,000 Competitive Projects Cooper-White Dr Lizbeth Grondahl, A/Prof platforms for tissue specific biomaterials Grant Income Ernst Wolvetang development and tissue genesis Australian ARC Discovery Dr Shizhang Dr Yonggang Jin, Prof Multifunctional porous panospheres 2010-2012 $85,000 Competitive Projects Qiao Mietek Jaroniec engineered composite membranes for Grant Income hydrogen and methanol fuel cells Australian ARC Discovery Prof Sean Dr Hong Zhang, Prof Walter Function, mechanism and 2010-2012 $120,000 Competitive Projects Smith Thiel dynamics in fluorescent proteins: a Grant Income computational investigation Australian ARC Discovery Prof Dr Xiangdong Yao Practical hydrogen storage for fuel 2010-2012 $130,000 Competitive Projects Chengzhong cells electrical vehicles by confined Grant Income Yu ammonia borane system Australian ARC Discovery Prof Michael A/Prof Nigel McMillan Engineered polymer nanoparticles: 2009-2011 $130,000 Competitive Projects Monteiro a potent weapon against cancer Grant Income Australian ARC Discovery Dr Simon Prof Mark Kendall Non-invasive diagnosis using 2009-2011 $120,000 Competitive Projects Corrie micropatches that sample biomarkers Grant Income from skin Australian ARC Discovery Prof Kirill Novel approaches for structural and 2009-2011 $180,000 Competitive Projects Alexandrov functional analysis of the protein Grant Income complex cog, a tether that controls intra-golgi trafficking

Australian ARC Discovery Prof Michael Prof Virgil Percec Designer nanoreactors: an 2009-2011 $150,000 Competitive Projects Monteiro environmentally friendly solution for Grant Income polymer synthesis Australian ARC Discovery Prof Andrew Dr Kevin Jack, Dr Hui Peng Designed delivery novel hydrogels for 2009-2011 $130,000 Competitive Projects Whittaker drug delivery from precisely-structured Grant Income networks Australian ARC Discovery Dr Shizhang Prof Duong Do, Synthesis of unique mesoporous 2009-2013 $40,715 Competitive Projects Qiao Dr Greg Birkett graphitic carbons and their application Grant Income to fundamental problems in adsorption science

Australian ARC Discovery Dr Hong Prof Suresh Bhatia, Quantum induced kinetic molecular 2008-2010 $140,000 Competitive Projects Zhang A/Prof Xiu Song Zhao sieving of hydrogen isotopes in Grant Income nanoporous materials Australian ARC Discovery A/Prof Idriss Prof Traian Chirila, Generation of peptidomimetic 2008-2010 $180,000 Competitive Projects Blakey Dr David Hill, surfaces for biomaterials applications Grant Income Dr Craig Hawker Australian ARC Discovery Prof Andrew Professor Maree Smith, Novel polymeric microparticles for 2010-2013 $32,000 Competitive Projects Whittaker Dr Bruce Wyse, Dr Kristofer slow-release intrathecal delivery of Grant Income Thurecht analgesics

67 Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

Australian ARC Future Prof Michael Transformer 3D nanostructures: 2010-2013 $222,800 Competitive Fellowships Monteiro stimuli responsive polymers Grant Income Australian ARC Future Prof Novel synthesis and bio-applications 2009-2014 $197,200 Competitive Fellowships Chengzhong of functional macroporous ordered Grant Income Yu siliceous foams Australian ARC Future Dr Annette Designed peptides as functional 2010-2013 $171,600 Competitive Fellowships Dexter surfactants Grant Income Australian ARC Future Prof Mark Optimising the body’s immune 2010-2014 $222,800 Competitive Fellowships Kendall response with a nanopatch that Grant Income delivers biomolecules to the skin Australian ARC Linkage Projects Prof Lars Dr Stevens Brumbley, Redirecting carbon flow through 2010-2014 $635,000 Competitive Nielsen Dr Kristi Snell mesophyll and bundle sheath cells Grant Income of sugarcane to produce poly-3- hydroxybutyrate

Australian ARC Linkage Projects Dr Zhi Ping Prof Anh Nguyen, Tailoring nano-crystal suspensions for 2009-2012 $120,535 Competitive (Gordon) Xu Dr Longbin Huang extended ion supply to hydrophobic Grant Income and hydrophilic leaf surfaces Australian ARC Linkage Projects Prof Andrew Dr Idriss Blakey, Dr Kevin Advanced lithographic solutions using 2009-2012 $484,000 Competitive Whittaker Jack, Prof John Drennan, block copolymers: integrating self Grant Income Dr Todd Ross Younkin assembly and lithography Australian ARC Linkage Projects Dr Steve Reid Prof Lars Nielsen In vitro production of baculovirus 2009-2012 $250,000 Competitive biopesticides – a systems biology Grant Income approach Australian ARC Linkage Projects Prof Max Lu Prof Victor Rudolph, Nano and micro-scale engineering of 2010-2013 $395,000 Competitive Dr Ronggang Ding, mos2-based catalyst for conversion of Grant Income Dr Guo Xiong Wang syngas to ethanol Australian ARC Linkage Projects Prof Andrew Prof Maree Smith, Novel polymeric microparticles for 2010-2013 $180,000 Competitive Whittaker Dr Bruce Wyse, slow-release intrathecal delivery of Grant Income Dr Kristofer Thurecht analgesics Australian ARC Linkage Projects Prof Justin Dr Tanja Eindorf, Intelligent scaffolds and methods for 2009-2012 $444,389 Competitive Cooper-White Prof Gail Anderson repair of osteochondral defects Grant Income Australian ARC Linkage Projects Prof Max Lu Dr Shizhang Qiao, Porous silica-based nanocapsules 2008-2011 $222,416 Competitive Dr Brenton Peters, for targeted and controlled release of Grant Income Dr Michael Kennedy biocides Australian ARC Linkage Projects A/Prof Prof Maree Smith, Dr Bruce Development of chaperonin 2010-2013 $150,000 Competitive Stephen Wyse, Dr Trent Woodruff, 10-based second generation Grant Income Mahler Prof Paul Marie Gerard biopharmaceuticals for treatment of Curmi, Dr Dean Naylor, inflammatory diseases Dr Richard Brown Australian ARC Linkage Projects Prof Peter Prof Bhesh Bhandari A novel rheological and chewing 2009-2012 $152,500 Competitive Halley and swallowing model for the smart Grant Income design of texture-modified foods for increased aged health

Australian Australian Institute of Dr Lizhong He The physical states of pharmaceutical 2008-2011 $165,982 Competitive Nuclear Science and proteins and self-assembled peptides Grant Income Engineering (AINSE Research Fellowship) Australian NHMRC Program Prof Kirill Prof Robert Parton Molecular and functional 2008-2012 $935,766 Competitive Grant Alexandrov characterisation of cell surface Grant Income microdomains Australian NHMRC Project Grant Prof Justin Prof Dietmar Hutmacher, Taking the limp out of cartilage repair 2010-2012 $144,375 Competitive Cooper-White Dr Michael Doran, Dr Garry Grant Income Brooke, Prof Julie Campbell Australian NHMRC Project Grant Prof Julie Dr Anita Thomas, Dr Antibody-directed delivery of anti- 2009-2011 $78,563 Competitive Campbell Zhiping Xu, Prof Max Lu restenotic agents using inorganic Grant Income nanoparticles Australian NHMRC Project Grant Prof Kirill Dr Daniel Abankwa Understanding changes in the 2009-2011 $181,250 Competitive Alexandrov mammalian prenylome induced by Grant Income statins and prenyltransferase inhibitors Australian NHMRC Project Grant Prof Mark Dr Germain Fernando, Prof Nanopatch immunisation against 2009-2011 $98,250 Competitive Kendall Lorena Brown, Prof Ian pandemic influenza: improved Grant Income Frazer, Dr Dexiang Chen immune responses at a reduced dose

68 Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

Australian NHMRC Project Grant Prof Julie Dr Sharon Ricardo Transplanted metanephroi as 2008-2010 $98,500 Competitive Campbell functional kidneys Grant Income Australian NHMRC Research Prof Julie Senior principal research fellowship 2006-2010 $158,675 Competitive Fellowship Campbell Grant Income Australian NHMRC Training Dr Bei Cheng NHMRC Australia-China exchange: 2009-2011 $188,190 Competitive (Postdoctoral) how does oestrogen affect blood Grant Income Fellowship vessels? Australian ARC Centres of Prof Sean ARC Centre for Functional 2003-2010 $238,667 Competitive Excellence Smith Nanomaterials – Computational Grant Income nanomaterials sciences Australian ARC Centres of Prof Matt Trau ARC Centre for Functional 2003-2010 $92,066 Competitive Excellence Nanomaterials – Healthcare Grant Income Australian ARC Centres of Prof Max Lu ARC Centre for Functional 2003-2010 $100,000 Competitive Excellence Nanomaterials – Healthcare and Grant Income environmental technologies National and Australian Academy of Dr Zhen Li Preparation and bioapplication of 2010 $4,100 International Science multifunctional magnetic nanoparticles Grant Income – AAS scientific visits to China National and Australian Academy of Dr Dawei Australian Academy of Science 2010 $5,000 International Science Wang scientific visits to North America Grant Income program National and Australian Stem Cell Dr Nick Prof Michael Atkinson, Development of a scalable, 2009-2011 $165,000 International Centre Timmins Dr Garry Brooke automated, closed system device Grant Income for manufacturing clinical grade mesenchymal stem cells

National and Australian Stem Cell A/ Prof Ernst Novel methods of reprogramming 2009-2011 $310,228 International Centre Wolvetang (ASCC collaborative stream 2, Grant Income module 1) National and Australian Stem Cell A/ Prof Ernst Prof Nicholas Fisk, Primitive ips-derived MSC for bone 2010-2011 $145,767 International Centre Wolvetang Dr Liza-Jane Raggatt repair (ASCC collaborative stream 2 Grant Income module 7) National and Australian Stem Cell A/ Prof Ernst Safe and efficient expansion of 2009-2011 $375,018 International Centre Wolvetang genetically stable hesc (ASCC Grant Income collaborative stream 1-module 6) National and Australian Stem Cell Prof Lars Production of neutrophils (ASCC 2009-2011 $518,532 International Centre Nielsen collaborative stream 1-module 4) Grant Income National and Australian Stem Cell Prof Peter A/ Prof Ernst Wolvetang, AIBN bioreactor program (ASCC 2009-2011 $808,212 International Centre Gray Prof Justin Cooper-White collaborative stream 1-module 2) Grant Income National and Australian Stem Cell A/ Prof Ernst Safe and efficient expansion of 2008-2010 $89,179 International Centre Wolvetang genetically stable hesc under defined Grant Income conditions (ASCC project p090) National and Australian Stem Cell Prof Lars Dr Nicholas Timmins, Ex vivo generated allogeneic 2008-2010 $234,262 International Centre Nielsen Dr Sia Athanasas-Platsis, neutrophils for anti-infective Grant Income Flavia Marturana, Penny supportive care in acute leukaemia Buntine National and Bill & Melinda Gates Dr Krassen Nano-dumbbells for single-molecule 2010-2011 $108,175 International Foundation Dimitrov diagnostics from saliva Grant Income National and Cancer Australia Prof Matt Trau A/Professor Melissa Brown, Nanoscaled biosensors: reading 2008-2011 $88,000 International Dr Glenn Francis, Dr epigenetic signatures to improve Grant Income Kymberley Vickery, breast cancer detection and treatment Dr Bronwyn Battersby National and CRC for Polymers Prof Peter A/Prof Rowan Truss, Prof Degradable packaging materials 2005-2012 $161,975 International Halley Mike Gidley, A/Prof Darren derived from renewable resources Grant Income Martin, Dr Fengwei Xie, Luke Matthew, Grant Edwards, Stephen Coombs, Robert Shanks

National and CRC for Sugar Industry Prof Lars Dr Stevens Brumbley The production of PHB/PHAs in plants 2006-2010 $750,740 International Innovation through Nielsen Grant Income Biotechnology

69 Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

National and Defence Materials Prof Peter Matthew Dargusch, Aircraft prognostic tools to reduce 2008-2015 $213,997 International Technology Centre Halley Prof Graeme George, corrosion impacts (DMTC) Grant Income A/Prof Martin Veidt National and Department of Prof John Prof Jin Zou Synthesis, characterisation, and 2010-2011 $54,780 International Innovation, Industry, Drennan applications of novel porous materials Grant Income Science and Research with complicated structures – ISL Australia-China Special Fund National and Go8 Australia- Prof Sean Dr Harendra Parekh Complexation and cellular uptake 2010-2011 $10,518 International Germany Joint Smith of genes with novel peptide-based Grant Income Research Co- dendrimers: a fundamental joint operation Scheme study involving synthesis, optical single-molecule spectroscopy and simulations

National and National Breast Prof Matt Trau Prof John Forbes, Prof Novel strategies for prediction and 2008-2013 $825,000 International Cancer Foundation Susan Clark, Prof Melissa control of advanced breast cancer Grant Income Brown, A/Prof Glenn Francis, via nanoscaled epigenetic-based Prof Alexander Dobrovic, biosensors Prof Rodney Scott, Dr Bronwyn J Battersby, Dr Kymberley Vickery

National and Qld Department of Prof Max Lu Dr Shizhang Qiao Platform technology for nanoparticle 2008-2011 $38,500 International Primary Industries & based non-injectible delivery of Grant Income Fisheries veterinary vaccines National and Queensland Prof Peter National Collaborative Research 2007-2011 $417,450 International Government Gray Infrastructure Strategy (NCRIS)- Grant Income capability area 5.5 biotechnology products

National and Queensland Prof Lars National Collaborative Research 2007-2011 $137,500 International Government Nielsen Infrastructure Strategy (NCRIS)- Grant Income capability area 5.1 evolving bio- molecular platforms and informatics (sub-capability-metabolomics)

National and Queensland Dr Claudia Smart Futures Fellowship: engineering 2010-2013 $55,000 International Government Smart Vickers sucrose-based industrial isoprenoid Grant Income Futures Fellowships production in yeast cells National and Queensland Dr Chamindie Smart Futures Fellowship: saving 2010-2013 $110,000 International Government Smart Punyadeera hearts with a simple saliva test Grant Income Futures Fellowships National and Queensland Dr Simon Smart Futures Fellowship: 2009-2012 $55,000 International Government Smart Corrie micropatches for non-invasive disease Grant Income Futures Fellowships diagnostics National and Queensland Dr Zhen Li Smart Futures Fellowship: 2009-2012 $110,000 International Government Smart multifunctional magnetic nanomatrials: Grant Income Futures Fellowships robust contrast agents for detection and treatment of cancers

National and Queensland Dr Chenghua Smart Futures Fellowship: computer- 2009-2012 $110,000 International Government Smart Sun aided synthesis of high-performance Grant Income Futures Fellowships titanium dioxide for solar cells and photocatalysts

National and Queensland Professor Delivering smarter vaccines faster 2010-2015 $250,000 International Government Smart Anton thorugh nanotechnology Grant Income Futures Premiers Middelberg Fellowships National and Queensland Prof Lars Prof Peter Gray, Queensland bio jet fuel initiative 2009-2011 $110,000 International Government Smart Nielsen Jason Fletcher Grant Income Futures Partnerships- Alliances Facilitation Program

National and Queensland Prof Peter Prof Lars Nielsen, Process modelling applicable to 2009-2011 $55,000 International Government Smart Gray Jason Fletcher biofuels Grant Income Futures Partnerships- Alliances Facilitation Program

National and Queensland Prof Andrew Dr Firas Rasoul, Prof Ian Spinal cord repair 2010-2013 $181,063 International Government Smart Whittaker Brereton, Dr Bronwin Grant Income State National and Dargaville, Assoc Prof John International Research Forsythe, Dr David Nisbet, Alliances Program Dr Eve Tsai

70 Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

National and Queensland Prof Anton Prof Sun Yan Vaccine now – beating infectious 2010-2013 $132,000 International Government Smart Middelberg disease with rapid response Grant Income State National and technology International Research Alliances Program National and Queensland Prof Lars Prof Peter P Gray, Brad Queensland sustainable aviation fuel 2010-2013 $495,000 International Government Smart Nielsen Wheatley, Dr Claudia E initiative Grant Income State National and Vickers, Prof Rocky De International Research Nys, A/Prof Ben Hankamer, Alliances Program Dr Ralf Dietzgen, Prof Peter M Gresshoff, Dr Neil Renninger

National and Queensland Prof Max Lu Professor Sean Smith, Queensland-China Alliance in 2008-2012 $804,375 International Government Smart Professor John Zhu, A/Prof Nanomaterials for Clean Energy Grant Income State National and Lianzhou Wiang, A/Prof Technologies (QCANCET) International Research Joe Diniz Da Costa Alliances Program National and Queensland Prof Mark Prof Ian Frazer, Prof International Needle-Free Vaccination 2009-2012 $454,861 International Government Smart Kendall Michael Roberts Alliance (INVAX) Grant Income State National and International Research Alliances Program

National and Queensland Dr Krassen Prof Karl Bohringer, Dr Molecular diagnostics for tropical 2009-2011 $536,250 International Government Smart Dimitrov Daniel Schwartz disease Grant Income State National and International Research Alliances Program

National and Queensland Prof Lars Prof Sang Yup Lee Korea-Australia Bio-Product Alliance 2008-2012 $855,671 International Government Smart Nielsen Grant Income State National and International Research Alliances Program

National and Queensland Prof Matt Trau Prof Leland (Lee) Hartwell, Novel nanotechnology platforms for 2007-2010 $467,500 International Government Smart Prof Kenneth D Stuart, Prof disease biomarker diagnostics Grant Income State National and Nancy B Kiviat International Research Alliances Program National and Queensland Prof Andrew Dr Firas Rasoul, Dr Anne International Biomaterials Research 2007-2011 $457,526 International Government Smart Whittaker Symons, Dr Craig Hawker, Alliance Grant Income State National and Prof Karen Wooley, Prof International Research Julie Campbell, Prof Alliances Program Traian Chirila, Prof David Haddleton, A/Prof Stephen Rose, Prof Steven Howdle

National and Queensland Prof Peter Prof Lars Nielsen, Bionano-products development 2007-2011 $2,721,057 International Government Smart Gray Prof Justin Cooper-White, facility Grant Income State Research Prof Peter Halley, Prof Max Facilities Fund Lu, Prof Anton Middelberg, Prof Andrew Whittaker, Prof Michael Monteiro, Prof Matt Trau, Dr Steve Reid, Prof Jonathan Golledge, Dr Stuart Hazell

National and Rural Industries Dr Akshat Dr Jorge Beltramini Conversion of lignocellulosic biomass 2010-2013 $82,500 International Research & Tanksale to dimethyl ether (BioDMR) Grant Income Development Corporation

National and Rural Industries Prof Justin A/Prof Paul Mills Modulation of gap junction expression 2007-2011 $11,000 International Research & Cooper-White in healing equine tendon Grant Income Development Corporation

National and Seattle Biomedical Prof Matt Trau Prof Gerard Cangelosi Accelerated molecular probe pipeline 2009-2011 $81,774 International Research Institute Grant Income

National and University of Sydney A/Prof Darren Linked centre and services (source of 2009-2012 $55,550 International Martin funds: NCRIS) Grant Income

71 Lead AIBN Other Chief 2010 Type Scheme Project Title Duration Investigator Investigators Income

National and Department of Prof Justin NCRIS fabrication 2007-2011 $1,400,000 International Innovation, Industry, Cooper White Grant Income Science and Research (DIISR)

National and Department of Prof Peter NCRIS biotech products 2007-2011 $700,000 International Innovation, Industry, Gray Grant Income Science and Research (DIISR)

National and Department of Prof Lars NCRIS-metabolomics 2007-2011 $121,500 International Innovation, Industry, Nielsen Grant Income Science and Research (DIISR)

National and Department Prof Justin EIF ANFF equipment 2010 $1,000,000 International of Innovation, Cooper White Grant Income Industry, Science and Research (DIISR) Equipment Infrastructure Funding (EIF)

Contract Queensland Prof Peter Hendra virus 2010 $300,000 Research Government Gray and other Industry Income

Contract Aortech A/Prof Darren Next generation nanocomposite 2008-2010 $10,000 Research Martin insulation materials for cochlear and other electrode arrays Industry Income Contract Cochlear A/Prof Darren Next generation nanocomposite 2008-2010 $44,500 Research Martin insulation materials for cochlear and other electrode arrays Industry Income Contract Uniquest Pty Ltd A/Prof Darren Next generation nanocomposite 2008-2010 $3,071 Research Martin insulation materials for cochlear and other electrode arrays Industry Income Contract Uniquest Pty Ltd Dr Zhi Ping Prof Justin Cooper-White, Coated IDH for RNAI delivery 2010 $32,550 Research (Gordon) Xu A/Prof Nigel McMillan and other Industry Income

Contract Uniquest Pty Ltd Prof Matt Trau Pathfinder forward osmosis 2010 $20,200 Research and other Industry Income

Contract Uniquest Pty Ltd Dr Annette Pepfactants 2010 $85,242 Research Dexter and other Industry Income

Contract Uniquest Pty Ltd A/Prof Bioproton phytase enzymes 2010-2011 $181,500 Research Stephen and other Mahler Industry Income Contract Incitec Pivot Ltd, Dr Zhi Ping Dr Longbin Huang, Preparation of cross-linking starch- 2010-2011 $36,667 Research Southern Cross (Gordon) Xu A/Prof Rowan W Truss based nanocomposite films and and other Operations characterisation of water adsorption Industry and mechanical strength Income

72 publications

Book Chapters Journal Articles 12. Chen, J. S., Luan, D. Y., Li, C. M., Boey, F. Y. C., Qiao, S. Z., and Lou, X. W. (2010) TiO 1. Chan, L., Reid, S. and Nielsen, L. K. (2010). 1. Aljada, M., and Asthana, A. (2010) Fabrication 2 and SnO @TiO hollow spheres assembled Baculovirus kinetics, insect cell culture, in of multilayer microstructures using dry film resist 2 2 from anatase TiO nanosheets with enhanced Encyclopedia of industrial biotechnology: and deep reactive ion etcher, Micro & Nano 2 lithium storage properties, Chemical Bioprocess, bioseparation, and cell technology. Letters 5, 121-124. Flickinger, M. C. (Ed.), (pp. 1-24) Hoboken, N.J. Communications 46, 8252-8254. 2. Bell, C. A., Bernhardt, P. V., Gahan, L. R., USA: John Wiley and Sons Inc. 13. Chen, J. S., Zhu, T., Hu, Q. H., Gao, J. J., Martinez, M., Monteiro, M. J., Rodriguez, Su, F. B., Qiao, S. Z., and Lou, X. W. (2010) 2. Halley, P. J. (2010). Morphology development C., and Sharrad, C. A. (2010) Copper(II) Shape-controlled Synthesis of Co3O4 in thermoset nanocomposites, in Optimization Complexes of a Hexadentate Mixed- of polymer nanocomposite properties. Mittal, V Nanocubes, Nanodiscs and Nanoflowers and Donor N3S3 Macrobicyclic Cage: Facile (Ed.), (pp. 21-40) Weinheim, Germany: Wiley-V Their Comparative Lithium Storage Properties, Rearrangements and Interconversions, C H Verlag GmbH & Co. Acs Applied Materials & Interfaces 2, 3628- Chemistry-A European Journal 16, 3166-3175. 3. Kendall, M. A. F. (2010). Needle-free vaccine 3635. 3. Bell, C. A., Sun, Q. A., Zhang, H., Smith, S. C., injection, in Drug delivery Schäfer-Korting, M. 14. Chen, Z. G., Cheng, L., Zou, J., Yao, X. D., Lu, Bernhardt, P. V., and Monteiro, M. J. (2010) (Ed.), (pp. 193-219) Heidelberg, Germany: G. Q., and Cheng, H. M. (2010) Zinc sulfide Methyl acrylate polymerizations in the presence Springer-Verlag. nanowire arrays on silicon wafers for field of a copper/N3S3 macrobicyclic cage in DMSO emitters, Nanotechnology 21, 065701. 4. Koops, Y., Brumbley, S.M, Poirier, A.J., de Laat, at 25 degrees C, Polymer Chemistry 1, 207- A., Scott, E., Sanders, J. and van der Meer, I.M. 212. 15. Chen, Z. G., Cheng, L. N., Lu, G. Q., and (2010). Production of chemical building blocks Zou, J. (2010) Sulfur-doped gallium phosphide by plants, The Biobased Economy: Biofuels, 4. Blakey, I., Schiller, T. L., Merican, Z., and nanowires and their optoelectronic properties, Materials and Chemicals in the Post-oil Era. In Fredericks, P. M. (2010) Interactions of Nanotechnology 21, 375701. Langeveld, H. Meeusen, M. and Sanders, J. Phenyldithioesters with Gold Nanoparticles (Ed.), (pp. 131-145) London, UK: Earthscan. (AuNPs): Implications for AuNP 16. Chen, Z. G., Cheng, L. N., Xu, H. Y., Liu, J. Z., Functionalization and Molecular Barcoding of 5. Lee-Wang, H. H., Blakey, I., Chirila, T. V., Peng, Zou, J., Sekiguchi, T., Lu, G. Q., and Cheng, H., Rasoul, F., Whittaker, A. K., and Dargaville, AuNP Assemblies, Langmuir 26, 692-701. H. M. (2010) ZnS Branched Architectures as Optoelectronic Devices and Field Emitters, B. L. (2010) Novel Supramolecular Hydrogels 5. Blakey, I., Thurecht, K. J., and Whittaker, A. Advanced Materials 22, 2376-2380. as Artificial Vitreous Substitutes, in Modern K. (2010) High-pressure real-time Xe-129 Trends in Polymer Science-EPF 09 Stelzer, F., NMR: monitoring of surfactant conformation 17. Chong, B. F., Abeydeera, W. P. P., Glassop, and Wiesbrock, E., Eds., pp 229-232. during the self-assembly of reverse micelles D., Bonnett, G. D., O’Shea, M. G., and 6. Nguyen, U. T. T., Goodall, A., Alexandrov, K., and in supercritical carbon dioxide, Chemical Brumbley, S. M. (2010) Co-ordinated synthesis Abankwa, D., (2010). Isoprenoid modifications, Communications 46, 2850-2852. of gentiobiitol and sorbitol, evidence of sorbitol glycosylation in transgenic sugarcane, in Post-translational modifications in health and 6. Blinco, J. P., Fairfull-Smith, K. E., Micallef, A. Phytochemistry 71, 736-741. disease. Vidal, C.J. (Ed.), (pp. 1-38) New York, S., and Bottle, S. E. (2010) Highly efficient, U.S.A.: Springer Science & Business Media, LLC. stoichiometric radical exchange reactions using 18. Chung, T. L., Brena, R. M., Kolle, G., 7. Poirier, Y. and Brumbley, S. M. (2010). Metabolic isoindoline profluorescent nitroxides, Polymer Grimmond, S. M., Berman, B. P., Laird, P. engineering of plants for the synthesis of Chemistry 1, 1009-1012. W., Pera, M. F., and Wolvetang, E. J. (2010) polyhydroxyalkanaotes, in Plastics from Bacteria: Vitamin C promotes widespread yet specific 7. Cao, X. A., Wang, T., Ngo, K. D. T., and Lu, Natural functions and applications. Chen DNA demethylation of the epigenome in G. Q. (2010) Parametric Study of Joint Height G-Q (Ed.), [electronic resource] (pp. 187-211) human embryonic stem cells, Stem Cells 28, for a Medium-Voltage Planar Package, Ieee London: Springer Verlag. 1848-1855. Transactions on Components and Packaging 8. Qiao, S. Z., Liu, J. and Lu, G. Q. (Max) (2010). Technologies 33, 553-562. 19. Chung, T. L., Turner, J. P., Thaker, N. Y., Kolle, Synthetic Chemistry of Nanomaterials, in G., Cooper-White, J. J., Grimmond, S. M., 8. Chandler-Temple, A., Wentrup-Byrne, Modern Inorganic Synthetic Chemistry. Xu, R. Pera, M. F., and Wolvetang, E. J. (2010) E., Whittaker, A. K., and Grondahl, Pang, W. and Huo, Q. (Ed.), (pp. 479-506) Ascorbate promotes epigenetic activation of L. (2010) Graft Copolymerization of Amsterdam: Elsevier. CD30 in human embryonic stem cells, Stem Methoxyacrylethyl Phosphate onto Expanded Cells 28, 1782-1793. 9. Roberts, M. and Kendall, M. A. F. (2010). Poly(tetrafluoroethylene) Facial Membranes, Needle free delivery, in Topical and transdermal Journal of Applied Polymer Science 117, 3331- 20. Codamo, J., Munro, T. P., Hughes, B. S., Song, drug delivery. Benson H. A. E. (Ed.), (pp. 1-464) 3339. M., and Gray, P. P. (2010) Enhanced CHO S.l.: John Wiley & Son. Cell-Based Transient Gene Expression with 9. Chandler-Temple, A. F., Wentrup-Byrne, E., 10. Smart, S. K., Edwards, G. A. and Martin, the Epi-CHO Expression System, Molecular Griesser, H. J., Jasieniak, M., Whittaker, A. D. J. (2010). Thermoplastic polyurethane Biotechnology, Nov 20. (Epub ahead of print). K., and Grondahl, L. (2010) Comprehensive nanocomposites, in Rubber nanocomposites: Characterization of Grafted Expanded Preparation, properties and applications. 21. Connolly, A. R., Palanisamy, R., and Trau, Thomas, S. and Stephen, R. (Eds.), (pp. 239- Poly(tetrafluoroethylene) for Medical M. (2010) Quantitative considerations 254) Singapore: Wiley. Applications, Langmuir 26, 15409-15417. for suspension array assays, Journal of Biotechnology 145, 17-22. 11. Wang, D., Hill, D. J. T., Peng, H., Symons, A., 10. Chen, D., Hou, J., Yao, L. H., Jin, H. M., Varanasi, S., Whittaker, A. K., and Rasoul, F. Qian, G. R., and Xu, Z. P. (2010) Ferrite 22. Connolly, A. R., and Trau, M. (2010) Isothermal (2010) Development of Injectable Biodegradable materials prepared from two industrial wastes: Detection of DNA by Beacon-Assisted Multi-Arms PEG-Based Hydrogels: Swelling and Electroplating sludge and spent pickle liquor, Detection Amplification, Angewandte Chemie- Degradation Investigations, in Modern Trends Separation and Purification Technology 75, International Edition 49, 2720-2723. in Polymer Science-EPF 09 Stelzer, F., and 210-217. 23. Corbett, H. J., Fernando, G. J. P., Chen, X. Wiesbrock, E., (Eds.), pp 233-237. 11. Chen, J. L., Zhu, Z. H., Wang, S. B., Ma, Q., F., Frazer, I. H., and Kendall, M. A. F. (2010) 12. Zhang H., Sun Q., Wang S., Olsen S., and Smith Rudolph, V., and Lu, G. Q. (2010) Effects of Skin Vaccination against Cervical Cancer S. C. (2010) Theoretical Studies of Green and nitrogen doping on the structure of carbon Associated Human Papillomavirus with a Novel Red Fluorescent Proteins, in Hydrogen Bonding nanotubes (CNTs) and activity of Ru/CNTs Micro-Projection Array in a Mouse Model, and Transfer in the Excited State. Han, K-L. and in ammonia decomposition, Chemical PLoS One 5, e13460 Zhao, G-J. (Eds.), John Wiley & Sons, Inc. Engineering Journal 156, 404-410.

73 24. Corrie, S. R., Fernando, G. J. P., Crichton, M. 39. Fernando, G. J. P., Chen, X. F., Prow, T. W., 52. Hartono, S. B., Qiao, S. Z., Liu, J., Jack, K., L., Brunck, M. E. G., Anderson, C. D., and Crichton, M. L., Fairmaid, E. J., Roberts, M. Ladewig, B. P., Hao, Z. P., and Lu, G. Q. M. Kendall, M. A. F. (2010) Surface-modified S., Frazer, I. H., Brown, L. E., and Kendall, M. (2010) Functionalized Mesoporous Silica with microprojection arrays for intradermal A. F. (2010) Potent Immunity to Low Doses Very Large Pores for Cellulase Immobilization, biomarker capture, with low non-specific of Influenza Vaccine by Probabilistic Guided Journal of Physical Chemistry C 114, 8353- protein binding, Lab on a Chip 10, 2655-2658. Micro-Targeted Skin Delivery in a Mouse 8362. Model, PLoS One 5, e10266 25. Crichton, M. L., Ansaldo, A., Chen, X. F., Prow, 53. He, C., Li, J. J., Li, P., Cheng, J., Hao, Z. T. W., Fernando, G. J. P., and Kendall, M. A. F. 40. Flegg, M. B., Poole, C. M., Whittaker, A. K., P., and Xu, Z. P. (2010) Comprehensive (2010) The effect of strain rate on the precision Keen, I., and Langton, C. M. (2010) Rayleigh investigation of Pd/ZSM-5/MCM-48 of penetration of short densely-packed theory of ultrasound scattering applied to composite catalysts with enhanced activity microprojection array patches coated with liquid-filled contrast nanoparticles, Physics in and stability for benzene oxidation, Applied vaccine, Biomaterials 31, 4562-4572. Medicine and Biology 55, 3061-3076. Catalysis B-Environmental 96, 466-475. 26. Dal’Molin, C. G. D., Quek, L. E., Palfreyman, R. 41. Fu, L. T., Chen, Z. G., Wang, D. W., Cheng, 54. He, C., Li, P., Cheng, J., Hao, Z. P., and Xu, W., Brumbley, S. M., and Nielsen, L. K. (2010) L. N., Xu, H. Y., Liu, J. Z., Cong, H. T., Lu, Z. P. (2010) A Comprehensive Study of Deep AraGEM, a Genome-Scale Reconstruction of G. Q., and Zou, J. (2010) Wurtzite P-Doped Catalytic Oxidation of Benzene, Toluene, Ethyl the Primary Metabolic Network in Arabidopsis, GaN Triangular Microtubes as Field Emitters, Acetate, and their Mixtures over Pd/ZSM-5 Plant Physiology 152, 579-589. Journal of Physical Chemistry C 114, 9627- Catalyst: Mutual Effects and Kinetics, Water Air 9633. and Soil Pollution 209, 365-376. 27. Dal’Molin, C. G. D., Quek, L. E., Palfreyman, R. W., Brumbley, S. M., and Nielsen, L. K. (2010) 42. Fu, L. T., Chen, Z. G., Zou, J., Cong, H. T., 55. He, L., Wang, H., Garamus, V. M., Hanley, C4GEM, a Genome-Scale Metabolic Model to and Lu, G. Q. (2010) Fabrication and visible T., Lensch, M., Gabius, H. J., Fee, C. Study C-4 Plant Metabolism, Plant Physiology emission of single-crystal diameter-modulated J., and Middelberg, A. (2010) Analysis 154, 1871-1885. gallium phosphide nanochains, Journal of of MonoPEGylated Human Galectin-2 Applied Physics 107, 124321. by Small-Angle X-ray and Neutron 28. Dexter, A. F. (2010) Interfacial and Emulsifying Scattering: Concentration Dependence Properties of Designed beta-Strand Peptides, 43. Fu, N., Li, Z., Myalitsin, A., Scolari, M., Weitz, of PEG Conformation in the Conjugate, Langmuir 26, 17997-18007. R. T., Butghard, M., and Mews, A. (2010) One- Biomacromolecules 11, 3504-3510. Dimensional Heterostructures of Single-Walled 29. Dietmair, S., Timmins, N. E., Gray, P. P., Nielsen, Carbon Nanotubes and CdSe Nanowires, 56. Heath, F., Saeed, A. O., Pennadam, S. L. K., and Kromer, J. O. (2010) Towards Small 6, 376-380. S., Thurecht, K. J., and Alexander, C. quantitative metabolomics of mammalian cells: (2010) ‘Isothermal’ phase transitions and development of a metabolite extraction protocol, 44. Gao, S. Y., Lees, J. G., Wong, J. C. Y., Croll, T. supramolecular architecture changes in Anal Biochem 404, 155-164. I., George, P., Cooper-White, J. J., and Tuch, thermoresponsive polymers via acid-labile B. E. (2010) Modeling the adhesion of human 30. Ding, T., Li, R., Zeitler, J. A., Huber, T. L., Gladden, side-chains, Polymer Chemistry 1, 1252-1262. embryonic stem cells to poly(lactic-co-glycolic L. F., Middelberg, A. P., and Falconer, R. J. acid) surfaces in a 3D environment, Journal 57. Hill, D. J. T., Whittaker, A. K., and Zainuddin, (2010) Terahertz and far infrared spectroscopy of Biomedical Materials Research Part A 92A, Z. (2010) Water diffusion into radiation of alanine-rich peptides having variable ellipticity, 683-692. crosslinked PVA-PVP network hydrogels, Optics Express 18, 27431-27444. Radiation Physics and Chemistry 80, 213-218. 45. Gazdag, E. M., Cirstea, I. C., Breitling, R., 31. Ding, Y., Chuan, Y. P., He, L., and Middelberg, Lukes, J., Blankenfeldt, W., and Alexandrov, K. 58. Hudson, J. E., Frith, J. E., Donose, B. C., A. P. (2010) Modeling the competition between (2010) Purification and crystallization of human Rondeau, E., Mills, R. J., Wolvetang, E. J., aggregation and self-assembly during virus- Cu/Zn superoxide dismutase recombinantly Brooke, G. P., and Cooper-White, J. J. (2010) like particle processing, Biotechnology and produced in the protozoan Leishmania A synthetic elastomer based on acrylated Bioengineering 107, 550-560. tarentolae, Acta Crystallogr Sect F Struct Biol polypropylene glycol triol with tunable 32. Dong, G. C., Chen, X., Zhang, X. J., Ngo, K. Cryst Commun 66, 871-877. modulus for tissue engineering applications, D. T., and Lu, G. Q. (2010) Thermal fatigue Biomaterials 31, 7937-7947. 46. George, P. A., Quinn, K., and Cooper- behaviour of Al2O3-DBC substrates under White, J. J. (2010) Hierarchical scaffolds via 59. Hudson, J. E., Mills, R. J., Frith, J. E., Brooke, high temperature cyclic loading, Soldering & combined macro and micro-phase separation, G., Jaramillo-Ferrada, P., Wolvetang, E. J., Surface Mount Technology 22, 43-48. Biomaterials 31, 641-647. and Cooper-White, J. J. (2010) A defined 33. Doran, M. R., Frith, J. E., Prowse, A. B., medium and substrate for expansion of human 47. Giraudet, S., Zhu, Z. H., Yao, X. D., and Fitzpatrick, J., Wolvetang, E. J., Munro, T. P., mesenchymal stromal cell progenitors that Lu, G. Q. (2010) Ordered Mesoporous Gray, P. P., and Cooper-White, J. J. (2010) enriches for osteo-genic and chondro-genic Carbons Enriched with Nitrogen: Application Defined high protein content surfaces for stem precursors, Stem Cells Development 20, 77- to Hydrogen Storage, Journal of Physical cell culture, Biomaterials 31, 5137-5142. 87. Chemistry C 114, 8639-8645. 34. Dou, B. J., Li, J. J., Hu, Q., Ma, C. Y., He, C., Li, 60. Hulicova-Jurcakova, D., Seredych, M., Jin, 48. Glushenkov, A. M., Hulicova-Jurcakova, D., P., Hu, Q. H., Hao, Z. P., and Qiao, S. Z. (2010) Y. G., Lu, G. Q., and Bandosz, T. J. (2010) Llewellyn, D., Lu, G. Q., and Chen, Y. (2010) Hydrophobic micro/mesoporous silica spheres Specific anion and cation capacitance in Structure and Capacitive Properties of Porous assembled from zeolite precursors in acidic porous carbon blacks, Carbon 48, 1767-1778. Nanocrystalline VN Prepared by Temperature- media for aromatics adsorption, Microporous Programmed Ammonia Reduction of V2O5, 61. Jambrina, P. G., Aoiz, F. J., Bulut, N., Smith, and Mesoporous Materials 133, 115-123. Chemistry of Materials 22, 914-921. S. C., Balint-Kurti, G. G., and Hankel, M. 35. Du, A. J., Zhu, Z. H., and Smith, S. C. (2010) The dynamics of the H+ + D-2 reaction: 49. Gu, Z., Rolfe, B. E., Xu, Z. P., Thomas, A. (2010) Multifunctional Porous Graphene for a comparison of quantum mechanical C., Campbell, J. H., and Lu, G. Q. (2010) Nanoelectronics and Hydrogen Storage: wavepacket, quasi-classical and statistical- Enhanced effects of low molecular weight New Properties Revealed by First Principle quasi-classical results, Physical Chemistry heparin intercalated with layered double Calculations, Journal of the American Chemical Physics 12, 1102-1115. hydroxide nanoparticles on rat vascular Chemical Society 132, 2876-2877. smooth muscle cells, Biomaterials 31, 5455- 62. Jiao, Y., Du, A. J., Zhu, Z. H., Rudolph, V., 36. Du, H. J., Ye, J. S., Zhang, J. Q., Huang, X. D., 5462. and Smith, S. C. (2010) A density functional and Yu, C. Z. (2010) Graphene Nanosheets theory study of CO and N-2 adsorption on 50. Haque, E., Bhandari, B. R., Gidley, M. J., 2 Modified Glassy Carbon Electrode as a Highly aluminium nitride single walled nanotubes, Deeth, H. C., Moller, S. M., and Whittaker, A. Sensitive and Selective Voltammetric Sensor Journal of Materials Chemistry 20, 10426- K. (2010) Protein Conformational Modifications for Rutin, Electroanalysis 22, 2399-2406. 10430. and Kinetics of Water-Protein Interactions in 37. Falconer, R. J., Marangon, M., Van Sluyter, S. Milk Protein Concentrate Powder upon Aging: 63. Jiao, Y., Du, A. J., Zhu, Z. H., Rudolph, C., Neilson, K. A., Chan, C., and Waters, E. Effect on Solubility, Journal of Agricultural and V., and Smith, S. C. (2010) Adsorption of J. (2010) Thermal Stability of Thaumatin-Like Food Chemistry 58, 7748-7755. Carbon Dioxide and Nitrogen on Single-Layer Protein, Chitinase, and Invertase Isolated from Aluminum Nitride Nanostructures Studied by 51. Haque, P., Barker, I. A., Parsons, A., Thurecht, Sauvignon blanc and Semillon Juice and Their Density Functional Theory, Journal of Physical K. J., Ahmed, I., Walker, G. S., Rudd, C. Role in Haze Formation in Wine, Journal of Chemistry C 114, 7846-7849. D., and Irvine, D. J. (2010) Influence of Agricultural and Food Chemistry 58, 975-980. 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74 65. Jones, M. L., Seldone, T., Smede, M., 79. Lashtabeg, A., Bradley, J. L., Vives, G., and 93. Lim, W. T., Seo, S. M., Wang, L. Z., Lu, G. Q., Heo, Linville, A., Chin, D. Y., Barnard, R., Mahler, Drennan, J. (2010) The effects of templating N. H., and Seff, K. (2010) Single-crystal structures S. M., Munster, D., Hart, D., Gray, P. P., synthesis procedures on the microstructure of of highly NH4+-exchanged, fully deaminated, and and Munro, T. P. (2010) A method for Yttria Stabilised Zirconia (YSZ) and NiO/YSZ fully Tl+-exchanged zeolite Y (FAU, Si/Al=1.56), all rapid, ligation-independent reformatting of templated thin films, Ceramics International 36, fully dehydrated, Microporous and Mesoporous recombinant monoclonal antibodies, Journal of 653-659. Materials 129, 11-21. Immunological Methods 354, 85-90. 80. Lawrie, K., Blakey, I., Blinco, J., Gronheid, R., 94. Ling, Y. C., Zhou, L. A., Tan, L., Wang, Y. H., 66. Kask, A. S., Chen, X., Marshak, J. O., Dong, Jack, K., Pollentier, I., Leeson, M. J., Younkin, and Yu, C. Z. (2010) Synthesis of urchin-like L., Saracino, M., Chen, D., Jarrahian, C., T. R., and Whittaker, A. K. (2010) Extreme CdWO4 microspheres via a facile template Kendall, M. A., and Koelle, D. M. (2010) DNA ultraviolet (EUV) degradation of poly(olefin sulfone) free hydrothermal method, CrystEngComm 12, vaccine delivery by densely-packed and short s: Towards applications as EUV photoresists, 3019-3026. microprojection arrays to skin protects against Radiation Physics and Chemistry 80, 236-241. 95. Liu, F., Yuan, P., Wan, J. J., Qian, K., Wei, G. vaginal HSV-2 challenge, Vaccine 28, 7483- 81. Le, S. J., Gongora, M., Zhang, B., Grimmond, F., Yang, J., Liu, B. H., Wang, Y. H., and Yu, C. 7491. S., Campbell, G. R., Campbell, J. H., and Z. (2010) Periodic Mesoporous Organosilicas 67. Keen, I., Lambert, L. K., Chirila, T. V., Paterson, Rolfe, B. E. (2010) Gene expression profile of with Controlled Pore Symmetries for Peptides S., and Whittaker, A. K. (2010) Degradable the fibrotic response in the peritoneal cavity, Enrichment., J. Nanosci. Nanotech (JNN-10- hydrogels for tissue engineering – Part Differentiation 79, 232-243. 094), publish online. I: Synthesis by raft polymerization and 82. Lee, H. K., Fragiadakis, D., Martin, D., 96. Liu, G., Niu, P., Sun, C. H., Smith, S. C., Chen, Z. characterization of PHEMA containing Milne, A., Milne, J., and Runt, J. (2010) G., Lu, G. Q., and Cheng, H. M. (2010) Unique enzymatically degradable crosslinks, Journal Dynamics of Uniaxially Oriented Elastomers Electronic Structure Induced High Photoreactivity of Biomimetics, Biomaterials and Tissue Using Broadband Dielectric Spectroscopy, of Sulfur-Doped Graphitic C3N4, Journal of the Engineering 6, 67-85. Macromolecules 43, 3125-3127. American Chemical Society 132, 11642-11648. 68. Kithva, P., Grondahl, L., Martin, D., and 83. Lee, J. 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Famaey, N., Martin, D., Campbell, J. H., Van Nouvel, C., Six, J. L., De Isla, N., Luo, L. 187. Wu, Y. W., Oesterlin, L. K., Tan, K. T., Oosterwyck, H., Meuris, B., and Flameng, H., Cooper-White, J., Rahouadj, R., and Waldmann, H., Alexandrov, K., and Goody, R. S. W. (2010) Cyclically stretching developing Wang, X. O. (2010) Aligned poly(L-lactic-co- (2010) Membrane targeting mechanism of Rab tissue in vivo enhances mechanical strength e-caprolactone) electrospun microfibers and GTPases elucidated by semisynthetic protein and organization of vascular grafts, Acta knitted structure: A novel composite scaffold probes, Nature Chemical Biology 6, 534-540. Biomaterialia 6, 2448-2456. for ligament tissue engineering, Journal of Biomedical Materials Research Part A 94A, 188. Wu, Y. Y., Chi, Y., Bai, H. M., Qian, G. R., 161. Su, Y. H., Qiao, S. Z., Yang, H. G., Yang, 1270-1282. Cao, Y. L., Zhou, J. Z., Xu, Y. F., Lu, Q., Xu, Z. C., Jin, Y. G., Stahr, F., Sheng, J. Y., Cheng, P., and Qiao, S. Z. (2010) Effective Removal L., Ling, C. Q., and Lu, G. Q. (2010) 174. Vickers, C. E., Blank, L. M., and Kromer, J. O. of Selenate from Aqueous Solutions by the Titanate-silica mesostructured nanocables: (2010) Grand Challenge Commentary: Chassis Friedel Phase, Journal of Hazardous Materials synthesis, structural analysis and biomedical cells for industrial biochemical production, 176, 193-198. applications, Nanotechnology 21, 065604 Nature Chemical Biology 6, 875-877. 189. Wu, Z. S., Wang, D. W., Ren, W., Zhao, J. 162. Sun, C. H., Du, A. J., Liu, G., Qiao, S. Z., Lu, 175. Wan, J. J., Qian, K., Zhang, J., Liu, F., Wang, P., Zhou, G. M., Li, F., and Cheng, H. M. G. Q., and Smith, S. C. (2010) Formation Y. H., Yang, P. Y., Liu, B. H., and Yu, C. Z. (2010) Anchoring hydrous RuO on graphene energies of low-indexed surfaces of tin (2010) Functionalized Periodic Mesoporous 2 sheets for high performance electrochemical dioxide terminated by nonmetals, Solid State Organosilicas for Enhanced and Selective capacitors, Advanced Functional Materials 20, Communications 150, 957-960. Peptide Enrichment, Langmuir 26, 7444- 3595-3602. 7450.

77 190. Wu, Z.-S., Ren, W., Wang, D. W., Li, F., Liu, Adsorption of Reduced Graphene Oxide onto Combined TG-DTA-MS Analyses, Journal of B., and Cheng, H. M. (2010) High-energy Self-Assembled Alkanethiol Monolayers on Gold Physical Chemistry C 114, 10768-10774. MnO Nanowire/Graphene and Graphene Electrodes: Tunable Electrode Dimension and 2 220. Zhang, J., Yu, M. H., Yuan, P., Wang, H. N., Asymmetric Electrochemical Capacitors., Acs Potential Electrochemical Applications, Journal of Qian, K., Tan, L., Wang, Y. H., and Yu, C. Z. Nano 4, 5835-5842. Physical Chemistry C 114, 4389-4393. (2010) Tuning cooperative vesicle templating 191. Xia, H. S., Lin, C. X., Zhou, C. H., Beltramini, J., 206. Ye, F., Mori, T., Ou, D. R., Zou, J., Drennan, and liquid crystal templating simply by varying Tong, D. S., and Yu, W. H. (2010) A magnetic J., Nakayama, S., and Miyayama, M. (2010) silica source, Journal of Materials Research zeolitic nanocomposite from occlusion of silica- Effect of nickel diffusion on the microstructure 25, 648-657. coated iron species by crystalline titanosilicate-1, of Gd-doped ceria (GDC) electrolyte film 221. Zhang, L., Geng, W. C., Qiao, S. Z., Zheng, H. J., Materials Letters 64, 2752-2754. supported by Ni-GDC cermet anode, Solid Lu, G. Q., and Yan, Z. F. (2010) Fabrication and State Ionics 181, 646-652. 192. Xia, H.-S., Zhou, C.-H., Tong, D., and Lin, C. Biosensing with CNT/Aligned Mesostructured (2010) Synthesis chemistry and application 207. Yu, A. G., Liu, H. P., Blinco, J. P., Jack, K. S., Silica Core-Shell Nanowires, Acs Applied development of periodic mesoporous Leeson, M., Younkin, T. R., Whittaker, A. K., Materials & Interfaces 2, 2767-2772. organosilicas, Journal of Porous Materials 17, and Blakey, I. (2010) Patterning of Tailored 222. Zhang, Y., Jin, Z., Wang, Y., and Cui, P. 225-252. Polycarbonate Based Non-Chemically (2010) Study on phosphorylated Zr-doped Amplified Resists Using Extreme Ultraviolet 193. Xie, F. W., Liu, W. C., Liu, P., Wang, J., Halley, hybrid silicas/PSF composite membranes Lithography, Macromolecular Rapid P. J., and Yu, L. (2010) Starch thermal for treatment of wastewater containing oil, Communications 31, 1449-1455. transitions comparatively studied by DSC and Journal of Membrane Science 361, 113-119. MTDSC, Starch-Starke 62, 350-357. 208. Yu, M. H., Yuan, P., Zhang, J., Wang, H. N., 223. Zheng, H. J., Tang, F. Q., Lim, M., Mukherji, Zhang, Y., Hu, Y. F., Wang, Y. H., and Yu, C. Z. 194. Xu, Y. F., Dai, Y. C., Zhou, J. Z., Xu, Z. P., Qian, G. A., Yan, X. X., Wang, L. Z., and Lu, G. (2010) A Bioinspired Route to Various Siliceous R., and Lu, G. Q. M. (2010) Removal efficiency Q. (2010) Multilayered films of cobalt Vesicular Structures, Journal of Nanoscience of arsenate and phosphate from aqueous oxyhydroxide nanowires/manganese oxide and Nanotechnology 10, 612-615. solution using layered double hydroxide nanosheets for electrochemical capacitor, materials: intercalation vs. precipitation, 209. Yu, S., Ahmadi, S., Sun, C., Palmgren, P., Journal of Power Sources 195, 680-683. Journal of Materials Chemistry 20, 4684-4691. Hennies, F., Zuleta, M., and GoÌthelid, M. 224. Zhou, G. M., Wang, D. W., Li, F., Zhang, L. L., Li, (2010) 4-tert-Butyl Pyridine Bond Site and 195. Xu, Y. F., Zhang, J., Qian, G. R., Ren, Z., Xu, N., Wu, Z. S., Wen, L., Lu, G. Q., and Cheng, H. Band Bending on TiO2(110), The Journal of Z. P., Wu, Y. Y., Liu, Q., and Qiao, S. Z. (2010) M. (2010) Graphene-Wrapped Fe3O4 Anode Physical Chemistry C 114, 2315-2320. Effective Cr(VI) Removal from Simulated Material with Improved Reversible Capacity Groundwater through the Hydrotalcite- 210. Yuan, P., Zhao, L. Z., Liu, N., Wei, G. F., and Cyclic Stability for Lithium Ion Batteries, Derived Adsorbent, Industrial & Engineering Wang, Y. H., Auchterlonie, G. J., Drennan, Chemistry of Materials 22, 5306-5313. Chemistry Research 49, 2752-2758. J., Lu, G. Q., Zou, J., and Yu, C. Z. (2010) 225. Zhou, J. Z., Wu, Y. Y., Liu, C., Orpe, A., Liu, Q. Solving hierarchical helical mesostructures 196. Xu, Z. P., and Braterman, P. S. (2010) A., Xu, Z. P., Qian, G. R., and Qiao, S. Z. (2010) by electron tomography, Chemical Synthesis, structure and morphology of Effective Self-Purification of Polynary Metal Communications 46, 1688-1690. organic layered double hydroxide (LDH) Electroplating Wastewaters through Formation hybrids: Comparison between aliphatic 211. Yuan, P., Zhao, L. Z., Liu, N. A., Wei, G. F., of Layered Double Hydroxides, Environmental anions and their oxygenated analogs, Applied Wang, Y. H., Auchterlonie, G. J., Drennan, Science & Technology 44, 8884-8890. Clay Science 48, 235-242. J., Lu, G. Q., Zou, J., and Yu, C. Z. (2010) 226. Zhou, L. A., Yang, L. C., Yuan, P., Zou, J., Evolution of Helical Mesostructures, 197. Yan, M., Mori, T., Zou, J., Auchterlonie, G. J., Wu, Y. P., and Yu, C. Z. (2010) alpha-MoO3 Chemistry-a European Journal 16, 1629-1637. and Drennan, J. (2010) Microstructural and Nanobelts: A High Performance Cathode chemical aspects of working-temperature 212. Zainuddin, Strounina, E. V., Hill, D. J. T., and Material for Lithium Ion Batteries, Journal of

aged Ca-doped CeO2, Journal of the Whittaker, A. K. (2010) Magnetization-prepared Physical Chemistry C 114, 21868-21872. European Ceramic Society 30, 2505-2513. NMR imaging of water penetration into 227. Zhou, L. A., Yu, M. M., Yang, J., Wang, Y. poly(vinyl alcohol)-poly(N-viny1-2-pyrrolidone) 198. Yan, M., Mori, T., Zou, J., Huang, H., and H., and Yu, C. Z. (2010) Nanosheet-Based hydrogels, Polymer International 59, 1520-1525. Drennan, J. (2010) Microstructures and Bi2MoxW1-xO6 Solid Solutions with mechanical properties of Ce1-xCaxO2-y 213. Zainuddin, Z., Chirila, T. V., Barnard, Z., Adjustable Band Gaps and Enhanced Visible- (x=0.05, 0.1, 0.2) with different sintering Watson, G. S., Toh, C., Blakey, I., Whittaker, Light-Driven Photocatalytic Activities, Journal temperatures, Journal of the European A. K., and Hill, D. J. T. (2010) F-2 excimer of Physical Chemistry C 114, 18812-18818. Ceramic Society 30, 669-675. laser (157 nm) radiation modification and 228. Zhou, L. A., Zhu, J., Yu, M. H., Huang, X. surface ablation of PHEMA hydrogels and the 199. Yan, X. X., Liu, G., Wang, L. Z., Wang, Y., D., Li, Z., Wang, Y. H., and Yu, C. Z. (2010) effects on bioactivity: Surface attachment and Zhu, X. F., Zou, J., and Lu, G. Q. (2010) MOxW1-xO3 center dot 0.3H(2)O Solid proliferation of human corneal epithelial cells, Antiphotocorrosive photocatalysts containing Solutions with Tunable Band Gaps, Journal of Radiation Physics and Chemistry 80, 219-229. CdS nanoparticles and exfoliated TiO2 Physical Chemistry C 114, 20947-20954. nanosheets, Journal of Materials Research 25, 214. Zaman, M., Skwarczynski, M., Malcolm, J. M., 229. Zhu, J., Tang, J. W., Zhao, L. Z., Zhou, X. F., 182-188. Urbani, C. N., Jia, Z., Batzloff, M. R., Good, Wang, Y. H., and Yu, C. Z. (2010) Ultrasmall, M. F., Monteiro, M. J., and Toth, I. (2010) Self- 200. Yan, X. X., Wei, G. F., Zhao, L. Z., Yi, J., Deng, Well-Dispersed, Hollow Siliceous Spheres adjuvanting polyacrylic nanoparticulate delivery H. X., Wang, L. Z., Lu, G. Q., and Yu, C. Z. with Enhanced Endocytosis Properties, Small system for group A streptococcus (GAS) (2010) Synthesis and in vitro bioactivity of 6, 276-282. vaccine, Nanomedicine: Nanotechnology, ordered mesostructured bioactive glasses Biology and Medicine 7, 168-173. 230. Zhu, Y. C., Liu, H. J., Li, F., Ruan, Q. C., Wang, with adjustable pore sizes, Microporous and H., Fujiwara, M., Wang, L. Z., and Lu, G. Q. Mesoporous Materials 132, 282-289. 215. Zeng, Q. H., Yu, A. B., and Lu, G. Q. (2010) (2010) Dipolar Molecules as Impellers Achieving Evaluation of Interaction Forces between 201. Yan, Z. F., Hao, Z. P., and Lu, G. Q. (2010) Electric-Field-Stimulated Release, Journal of the Nanoparticles by Molecular Dynamics Perspective on catalysis for sustainable American Chemical Society 132, 1450-1451. 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78 AIBN Seminars 2010

8 February: Professor Matthias Lutolf, Ecole 13 May: AIBN Seminar: Dr Amanda S Barnard, Polytechnique Fédérale de Lausanne, France Virtual Nanoscience Laboratory, CSIRO Materials Title: Designing materials to direct stem cell fate Science and Engineering, Melbourne, Australia Title: Predicting the properties of multi–functional 19 February: AIBN Special Seminar: Dr Jin nanoparticles for bio-applications Kawakita, Advanced Photovoltaics Center, the National Institute for Materials Science, Tsukuba, 19 May: AIBN Special Seminar: Professor O Japan. Savadogo, Laboratory of New Materials for Title: Introduction to photovoltaics research in NIMS Electrochemistry and Energy, École Polytechnique the Montréal, Canada 25 February: Professor Toshiro Ohashi, Title: Search for new families of electro catalysts Graduate School of Engineering, Hokkaido for the ORR cathodes University, Sapporo, Japan Title: Mechanical characterisation of vascular 20 May: Professor Yi-Bing Cheng, Department endothelial cells focusing on intracellular structures of Materials Engineering, Monash University, Melbourne, Australia 1 March: AIBN Special Seminar: Professor Title: Novel structures of dye-sensitised solar cell Mietek Jaroniec, Kent State University, Ohio, US devices Title: Major advances in chemistry of ordered nanoporous materials 27 May: Dr Suzanne V Smith, Senior Research Fellow, Node Director, Centre of Excellence in 11 March: Professor Martin Schwartz, Professor Antimatter Matter Studies, ANSTO, Sydney NSW of Microbiology and Biomedical Engineering, Title: Deploying nanotechnologies for the University of Virginia, US integration of PET and MRI in molecular imaging Title: Mechanotransduction and force transmission by integrins 3 June: Joint AIBN/QBI Seminar: Dr Muhammad Zaman, Department of Biomedical Engineering, 18 March: Professor Karl F Böhringer, Professor Boston University, US of Electrical Engineering; Adjunct Professor of Title: Quantifying cell-matrix interactions through Computer Science & Engineering and Mechanical materials and multi-scale modelling Engineering; Director, MEMS Laboratory Department of Electrical Engineering, University of 10 June: Associate Professor Peter Fredericks, Washington, US Chemistry, Faculty of Science & Technology, Title: Interfacial Engineering in Queensland University of Technology, Australia microelectromechanical systems: handling and Title: Vibrational spectroscopy in materials assembly of solids and liquids at the microscale science: applications in surface characterisation and processing 25 March: Dr Albert S Mellick, Head, Host Response to Cancer Lab, School of Medical 17 June: Professor John Prins, Mater Medical Science, Griffith University, Brisbane, Australia Research Institute, Brisbane, Australia Title: Endothelial progenitor cells and cancer Title: Obese Australia – understanding the biology of adipose tissue growth and implications for 15 April: Dr Kathryn Morris, Partner, Davies treatment Collison Cave, Brisbane, Australia Title: You may have an invention but is it 24 June: Professor Richard A Jefferson, commercially valuable – the untold perspective of Professor of Science, Technology & Law, a patent attorney Queensland University of Technology, Australia Title: Biological open source (BiOS): Innovation 22 April: Professor Mark Walker, School of and evolution in complex systems Chemistry & Molecular Bioscience, The University of Queensland, Australia 28 June: AIBN Special Seminar: Professor Title: Identification of safe and efficacious vaccines Patrick S Stayton, Department of Bioengineering, to prevent group A streptococcal infections University of Washington, US Title: Smart biohybrid materials that talk and listen 27 April: Professor Zhiguo Su, Director, National in nanospace Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese 8 July: Professor Rolf Müller, Helmholtz Centre Academy of Science, China for Infectious Research; Helmholtz-Institute for Title: A novel process for making polymer Pharmaceutical Research Saarland; Department microspheres and microcapsules Microbial Natural Products, Saarland University, Germany 29 April: Associate Professor Nigel McMillan, Title: Myxobacteria: cool bugs for novel drugs Deputy Director and Principal Research Fellow, Diamantina Institute for Cancer, Immunology and 15 July: Special Seminar: Professor Min Wang, Metabolic Medicine, The University of Queensland, Department of Chemistry, Zhenjiang University, Australia China Title: RNA interference: How do we deliver the Title: Fabrication of highly ordered goods? nanotopography and its applications for bioanalysis 4 May: AIBN Special Seminar: Professor David Edwards, Harvard University, US 26 July: Special Seminar: Dr Elsa Sanchez Title: Inhaled vaccines for infectious diseases in Garcia, Max-Planck-Institut für Kohlenforschung, low-income settings Mülheim an der Ruhr, Germany Title: QM/MM study of monomeric red fluorescent proteins

79 29 July: Special Seminar: Dr Paul Dalton, Institute 7 October: AIBN Special Seminar: Professor of Health and Biomedical Innovation, Queensland Hien Ngo, School of Dentistry, The University of University of Technology; Med-X Research Institute, Queensland, Australia Shanghai Jiao Tong University, Shanghai, China Title: Adhesive in dentistry: past, present and future Title: Materials and the neurosciences: New 8 October: AIBN Special Seminar: Professor directions with old challenges David Koelle, University of Washington, US 6 August: AIBN Special Seminar: Professor Lei Title: Human T-cell responses to large-genome Jiang, Centre of Molecular Sciences, Institute of viruses: use of unbiased genomic tools to capture Chemistry, Chinese Academy of Sciences, Beijing, 14 October: Dr. Ming S Liu, Research Scientist, China Molecular Simulation, CSIRO, Mathematics, Title: Bio-Inspired, smart, multiscale interfacial Informatics & Statistics, Melbourne, Australia materials Title: Operating mechanism and dynamics of 12 August: Dr Mike O’Shea, Principal Research multimeric proteins – Intrinsic fluctuation, dynamic Scientist, CSIRO Molecular and Health correlations and allosteric cooperativity Technologies, Melbourne, Australia 19 October: AIBN Special Seminar: Darin Title: Drug-polymer conjugates and polymer Zehrung, Technical Officer, Project Manager, scaffolds for biomedical applications and PATH, Seattle, US renewable opportunities for monomers, polymers Title: Innovative technology solutions for global and chemicals health: PATH’s product development approach 19 August: Associate Professor Alessandro and experience Martucci, Materials Science Engineering, 28 October: Professor Michael Monteiro, Dipartimento Ingegneria Meccanica Set. Materiali, AIBN Group Leader, AIBN, The University of Universita’ di Padova, Italy Queensland, Australia Title: Sol-gel nanocomposite films as gas sensing Title: Synthesis and self-assembly of complex and active optical materials polymer architectures into nanostructures 26 August: Dr James R McMillan, Centre for 4 November: AIBN Special Seminar: Associate Children’s Burns Research, The University of Professor Yin Xiao, Institute of Health and Queensland, Australia Biomedical Innovation, Queensland University of Title: Comparison of polymer and collagen-based Technology, Australia skin tissue engineering scaffolds Title: The identity of mesenchymal stem cells 26 August: Professor Pamela Silver, Department and their tissue forming capacity in hard tissue of Systems Biology, Harvard Medical School, Wyss regeneration Institute of Biologically Inspired Engineering, Harvard 5 November: AIBN Special Seminar: Professor Tao University, US Yi, Department of Chemistry, Fudan University, China Title: Designing biological systems Title: Stimulus responsive soft materials based on 2 September: Associate Professor John Albert supramolecular assembly Violet, Wellington Hospital, New Zealand 5 November: AIBN Special Seminar: Dr Richard Title: Antibody directed radiotherapy; CD25 CD20 Law, Principal Engineer, Product Development, and other targets Organovo, Inc, US 9 September: Associate Professor Michael Title: Product development, Organovo, Inc Raghunath, Division of Bioengineering; 8 November: AIBN Special Seminar: Professor Department of Biochemistry, National University of Dennis E Discher, School of Engineering and Singapore, Singapore Applied Science; Graduate Groups in Cell & Molecular Title: Reconstructing the stem cell microenvironment Biology and Physics, University of Pennsylvania, US by delegation – the liquid and the solid way Title: Matrix and myosin in cell fate decisions 9 September: AIBN Special Seminar: Professor 11 November: Dr Lars M Blank, Chemical P. Somasundaran, Columbia University, New Biotechnology, Faculty of Biochemical and Chemical York, US Engineering, TU Dortmund University, Germany Title: Exploration of nanobiointerface for Title: A new bioreactor concept – the envirostat understanding and mitigating nanotoxicity 18 November: Professor Raffaele Mezzenga, 16 September: Professor Melissa Little, Group ETH Zurich, Switzerland Leader, Institute for Molecular Bioscience, The Title: A journey into the physics of protein fibrils University of Queensland, Australia from their bulk properties to single molecule Title: Kidney regeneration: What are the options and how far have we come? 25 November: Barry Thomas, Vice President, Cook Medical Inc, Director Asia/Pacific, Brisbane, 23 September: Professor Chengzhong (Michael) Australia Yu, ARC Future Fellow, Group Leader, AIBN, The Title: Commercialisation in perspective: A medical University of Queensland, Australia devices experience Title: Understand the structure-property correlation and design nanostructures with 9 December: Dr Michael Johnston, University of advanced applications Oxford, UK Title: Ultrafast terahertz conductivity studies of 24 September: AIBN Special Seminar: Professor semiconductor nanowires Noboru Mizushima, Department of Physiology and Cell Biology Tokyo Medical and Dental 15 December: AIBN Special Seminar: Professor University, Tokyo Yateman Arryanto, Inorganic Material Research Title: Physiological role of autophagy and its Group, Faculty of Mathematic and Natural Sciences, regulation mechanism University of Gadjah Mada, Yogyakarta, Indonesia Title: Synthesis and applications of photocatalyst 30 September: Professor Robert Henry, Director and adsorbent material from natural bentonite and of Queensland Alliance for Agriculture and Food zeolite Innovation, The University of Queensland, Australia Title: Innovation in agriculture and food 16 December: AIBN Special Seminar: Dr Kiyoshi Ozawa, National Institute for Materials Science, 30 September: AIBN Special Seminar: Dr Lisbeth Ibaraki, Japan Grondahl, School of Chemistry and Molecular Title: Superior electrochemical charge and Biosciences, The University of Queensland, Australia discharge performance of the manganese-based Title: Use of polysaccharides in biomaterials layered cathode materials of Li[Lix/3M1-xMn2x/3] applications O2(M=Ni or Co)

80 OUR HISTORY: – AIBN was established by The University of Queensland Senate in December 2002. – Construction of a custom-designed 15,689sq m AIBN research facility started in November 2004. – First AIBN Group Leaders appointed in 2005. – The $73.6 million AIBN research facility was completed in August 2006. – Then Queensland Premier Peter Beattie opened the facility on October 23, 2006. 46948 JULY2011 PW 1500 • CRICOS PROVIDER NUMBER 00025B

The University of Queensland St Lucia, Brisbane Qld 4072 www.aibn.uq.edu.au