Mollisia Cortegadensis Fungal Planet Description Sheets 377

376 Persoonia – Volume 43, 2019

Mollisia cortegadensis Fungal Planet description sheets 377

Fungal Planet 1019 – 18 December 2019 Mollisia cortegadensis De la Peña-Lastra & P. Alvarado, sp. nov.

Etymology. The epithet refers to the place where it was found (Illa de 8–14 × 2.8–3.5 µm, but those of M. cortegadensis can be as Cortegada, Parque Nacional Marítimo-Terrestre de las Islas Atlánticas, long as 18 µm. In addition, M. cortegadensis is drought-tolerant Galicia, Spain). and growing in clusters in the apical part of small decorticated Classiﬁcation — Mollisiaceae, Helotiales, Leotiomycetes. branches of Quercus robur, while M. spectabilis grows on rotten leaves of Q. robur or underneath rotten Quercus logs Apothecia gregarious, from 0.5–4 mm diam, ﬁrst slightly con- (Kirschstein 1938). Other species similar to M. spectabilis such cave and then flattened, irregularly disc-shaped, umbilicated or as M. elegantior and M. olivascens can be found in the same depressed, sessile, centrally attached to the substrate. Hyme- locality, but they have different spore dimensions and lack the nium smooth, wavy, gibbous, yellowish grey when fresh and orange ochre tones when dry (Richter & Baral 2008, Le Gal & orange-ochre when dry, with the external and central parts dark Mangenot 1958). The putative phylogenetic relationship with grey. Asci cylindrical-clavate 80–120 × 12–18 μm, 8-spored, M. ligni and M. minutella is only supported by a few shared with a conical apex and a base gradually narrowed into a ecological or morphological trait, since M. ligni has ascospores medium-sized stalk with croziers, showing no reaction to IKI 6–10 × 2–3 µm, cylindrical paraphyses with low refractive (Lugol’s solution), and turning only slightly yellowish in KOH (no vacuoles (Karsten 1873) and M. minutella, which is sometimes ionomidotic reaction). Paraphyses distinctly dimorphic, either considered a synonym of M. cinerea, has ascospores 7–14 cylindrical, inflated (molliform), or slightly broadened at the × 2.5–3 µm and the apices of asci stain blue in IKI (Karsten apex, with refractive vacuolar bodies at the top. In addition, the 1871). paraphyses extend beyond the asci. Ascospores elliptical with rounded ends, measuring 15–18 × 5.5–7 μm, with 1–2 small (< 1 μm) guttules at the poles. Ectal excipulum consisting of a HME4375 Hydrocina chaetocladia brownish texture globose at the base, and globose elements G.M. 2016-01-06.1 Mollisia caespiticia 1.00/99 CBS 132.51 Cadophora gregata in the surface. The margin lacks conspicuous protruding cells. 1.00/99 CBS 165.42 Cadophora malorum The medullary tissue consists of gelatinized hyphae. Subicular CBS 141.41 Cadophora luteo-olivacea PLOETTNERULACEAE CBS 268.33 Cadophora melinii TYPE hyphae sparse, hyaline and thick-walled. All observations made 1.00/96 CBS 401.78 Mollisia dextrinospora on fresh specimens. CBS 312.61 Mollisia cinerella Distribution — Currently known only from the type location G.M. 2014-06-15.1 Patellariopsis atrovinosa CNF 2/10097 Pyrenopeziza velebitica in north-western Spain. 1.00/99 FMR 11236 Fuscosclera lignicola Phylogeny — The analysis of ITS and 28S rDNA suggests BHI-F752a Phialocephala oblonga CPC 24607 Cheirospora botryospora (0.84/31) that the sample from Cortegada is related with the monophyletic 1.00/100 CBS 109321 Acephala applanata Vibrissea-Loramyces clade (Wang et al. 2006, Hustad & Miller 1.00/98 KUS F52181 Mollisia ventosa 2011, Han et al. 2014) of the Mollisiaceae s.lat. According to the CBS 258.91 Vibrissea truncorum ILLS 60499 Vibrissea filisporia 1.00/98 VIBRISSEACEAE family concepts proposed by Johnston et al. (2019), M. corte- KUS F52561 Vibrissea filisporia 1.00/78 gadensis belongs to the clade of families Mollisiaceae, Loramy- 1.00/100 MBH39316 Vibrissea flavovirens MFLU 16-1862 Strossmayeria bakeriana 1.00/100 Strossmayeria cetaceae and Vibrisseaceae, which could be merged into the BHI-F627a Durella connivens 1.00/100 clade oldest name Mollisiaceae. G.M. 2015-05-16 Durella connivens

0.99/77 CBS 468.94 Phialocephala scopiformis Typus. Spain, Galicia, Pontevedra, Parque Nacional de las Islas Atlánti- 1.00/96 CPC 13571 Trimmatostroma salicis cas de Galicia, Illa de Cortegada, N42°36'59.65" W8°46'59.22", 9.4 m asl, CBS 282.74 Trimmatostroma betulinum 1.00/93 a group of ascomata at the tip of a dead attached twig of Quercus robur, OSC 100029 Mollisia cinerea 1.00/98 CBS 235.53 Loramyces macrosporus 27 Apr. 2016, S. De la Peña-Lastra (holotype MSS906, ITS and 28S/LSU CBS 293.52 Loramyces juncicola 1.00/100 sequences GenBank MN129025 and MN129020, MycoBank MB831739). CBS 358.58 Septoria tanaceti MFLU 18-1819 Pulvinata tomentosa 1.00/82 1.00/96 1.00/98 Notes — Mollisia cortegadensis is characterised by its two CBS 553.79 Obtectodiscus aquaticus types of paraphyses: slightly broadened at their tips and others MFLU 18-0701 Neomollisia gelatinosa 1.00/99 61R8 Acidomelania panicicola 1.00/99 strongly swollen, but all of them have conspicuous refractive CM16S1 Acidomelania panicicola vacuolar bodies that stain in cresyl blue. In addition, the fungus 0.98/82 CBS 486.48 Mollisia fusca MOLLISIACEAE 0.98/72 1.00/99 CBS 589.84 Mollisia melaleuca is drought-tolerant suggested by the dry photo and the inamyloid 53 OA-2013 Mollisia sp. 1.00/100 asci. Mollisia spectabilis has similar spore dimensions about ZK71/08 Mollisia minutella 75 OA-2013 Mollisia sp. ALV8039 Mollisia cortegadensis sp. nov.

1.00/100 TTT2390 Mollisia sp. TTT2395 Mollisia sp.

1.00/100 MFLU 16-0599 Neopyrenopeziza nigripigmentata G.M. 2017-09-04.3 Patellariopsis dennisii KUS F52417 Pyrenopeziza sp. Colour illustrations. Location where M. cortegadensis was collected on 1.00/100 BHI-F738a Mollisia ligni 0.02 0.99/82 BHI-F606a Mollisia ligni Cortegada Island. Fresh apothecia; dry apothecia; elements of the hymenium; spores (two of them in IKI at the bottom); paraphyses, hymenium in 50 % majority rule ITS-28S rDNA consensus phylogram of several lineages KOH; ascus in IKI (-); ascus in water, base of an ascus showing the crozier, in the Mollisioid clade (Johnston et al. 2019), including families Mollisiaceae, paraphyses; detail of paraphyses in cresyl blue, ectal excipulum; medullary Loramycetaceae and Vibrisseaceae obtained in MrBayes from 1 650 sam- excipulum; medullary excipulum in KOH, medullary excipulum in NH4OH; pled trees. Nodes were annotated if supported by ≥ 0.95 Bayesian PP (left) ascome margin; marginal cells; flanks. Scale bars = 50 μm (apothecia, ascus or ≥ 70 % ML BP (right). Non-signiﬁcant support values are exceptionally in water and medullary excipulum in KOH), 20 μm (other structures). represented inside parentheses.

Saúl De la Peña-Lastra, Departamento de Edafoloxía e Química Agrícola, Facultade de Biología, Universidade de Santiago de Compostela, 15782-Santiago de Compostela, Spain; e-mail: [email protected] Pablo Alvarado, ALVALAB, La Rochela 47, 39012 Santander, Spain; e-mail: [email protected]