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GADD45 Induction of a G2/M Cell Cycle Checkpoint
Proc. Natl. Acad. Sci. USA Vol. 96, pp. 3706–3711, March 1999 Cell Biology GADD45 induction of a G2/M cell cycle checkpoint XIN WEI WANG*, QIMIN ZHAN†,JILL D. COURSEN*, MOHAMMED A. KHAN*, H. UDO KONTNY†,LIJIA YU‡, M. CHRISTINE HOLLANDER†,PATRICK M. O’CONNOR‡,ALBERT J. FORNACE,JR.†, AND CURTIS C. HARRIS*§ *Laboratory of Human Carcinogenesis, †Laboratory of Biological Chemistry, and ‡Laboratory of Molecular Pharmacology, Division of Basic Science, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Communicated by Theodore T. Puck, Eleanor Roosevelt Institute for Cancer Research, Denver, CO, January 12, 1999 (received for review November 30, 1998) ABSTRACT G1yS and G2yM cell cycle checkpoints main- p53-deficient mice, p21-deficient mice undergo normal develop- tain genomic stability in eukaryotes in response to genotoxic ment and normal apoptotic response, and do not have an stress. We report here both genetic and functional evidence of a increased frequency of spontaneous malignancies (11, 16). These y Gadd45-mediated G2yM checkpoint in human and murine cells. data indicate that factors other than p21 in the G1 S checkpoint Increased expression of Gadd45 via microinjection of an expres- pathway may be required for p53-mediated tumor suppression. sion vector into primary human fibroblasts arrests the cells at Alternatively, other p53-mediated checkpoints may play more the G yM boundary with a phenotype of MPM2 immunoposi- important roles in the prevention of tumorigenesis. 2 y y tivity, 4n DNA content and, in 15% of the cells, centrosome In contrast to the G1 S checkpoint, the mammalian G2 M separation. The Gadd45-mediated G2yM arrest depends on checkpoint is poorly understood. -
Healthnet News V.19:No.1 Spring 2004 Judith S
University of Connecticut OpenCommons@UConn Articles - Patient Care Patient Care Spring 2004 Healthnet News v.19:no.1 Spring 2004 Judith S. Kronick University of Connecticut School of Medicine and Dentistry Alberta Richetelle University of Connecticut School of Medicine and Dentistry Follow this and additional works at: https://opencommons.uconn.edu/pcare_articles Part of the Medicine and Health Sciences Commons Recommended Citation Kronick, Judith S. and Richetelle, Alberta, "Healthnet News v.19:no.1 Spring 2004" (2004). Articles - Patient Care. 74. https://opencommons.uconn.edu/pcare_articles/74 HEALTHNET NEWS A newsletter for public librarians and others interested in consumer health information services Volume XIX Number 1 Spring 2004 UPDATE BIOMEDICINE AND HEALTH IN THE NEWS CEASES PUBLICATION Biomedicine and Health in the News, the database of research related news stories from the New York Times, has officially ceased publication. The database was created and maintained by Healthnet staff and appears on Healthnet’s homepage at http://library.uchc.edu/departm/hnet. New files have not been added to the database since the end of March. All of the backfiles are still available for viewing and searching. The database is ceasing publication because it has outlived its usefulness. When it first appeared it was one of just a few sources of news information about current medical research. Currently, there are numerous sites where a person can find information about medical research reported in the news. Within the next few weeks, we’ll be replacing Biomedicine and Health in the News with a page listing some of the best free medical news sites. -
95 CHAPTER 5 Looking at Science, Looking at You! the Feminist Re-Visions of Nature
CHAPTER 5 Looking at Science, Looking at You! The Feminist Re-visions of Nature (Brain and Genes) Cecilia Åsberg Vision has often been a central concern of feminist studies of science, medi- cine and technology. In cultural or social feminist analysis, the male gaze and the ways in which technoscience1 accommodates, and in effect organizes the watching of women, has been an important part of the feminist interrogation of the gender and power relations that produce the subjects and the objects of science.2 This attention is due to the intimate, and power-saturated, merge of processes of seeing and processes of knowing. Inherent in the notion of vision, there is always a politics to ways of seeing, ordering and observing, of organising the knowledge of the world. Historically, this can be exemplified by the eighteen-century Swedish “father” of biological classification, Linnaeus. Taking a leap away from Christian assumptions, Linnaeus placed human beings in a taxonomic order of nature together with other animals.3 In his large-scale vision, he located humans together with primates in the order of Homo sapiens, as Donna Haraway4 so eloquently describes it in her ground-breaking book Primate Visions. And as the “father” of a specific discourse on nature, one that was not understood biologically but rather representationally, and still within a highly Christian framework, he referred to himself as the second Adam, as the “eye” of God. As the second Adam, Linnaeus could give true representations and true names to nature’s creatures and in so doing also restore the purity of name-giving lost by the first, biblical Adam’s sin. -
Inhibition of Single Minded 2 Gene Expression Mediates Tumor-Selective Apoptosis and Differentiation in Human Colon Cancer Cells
Inhibition of Single Minded 2 gene expression mediates tumor-selective apoptosis and differentiation in human colon cancer cells Mireille J. Aleman*†‡§, Maurice Phil DeYoung*†§¶, Matthew Tress*†, Patricia Keating*†, Gary W. Perryʈ, and Ramaswamy Narayanan*†** *Center for Molecular Biology and Biotechnology, Departments of †Biology and ‡Chemistry, and ¶Center for Complex System and Brain Sciences, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431 Communicated by Herbert Weissbach, Florida Atlantic University, Boca Raton, FL, July 21, 2005 (received for review April 4, 2005) A Down’s syndrome associated gene, Single Minded 2 gene short bound AhR͞ARNT complex (12) and hence prevent carcinogen form (SIM2-s), is specifically expressed in colon tumors but not in metabolism, leading to cumulative DNA damage and cancer. the normal colon. Antisense inhibition of SIM2-s in a RKO-derived The growth arrest and DNA damage (GADD) family of genes colon carcinoma cell line causes growth inhibition, apoptosis, and was originally isolated from UV radiation-treated cells and subse- inhibition of tumor growth in a nude mouse tumoriginicity model. quently grouped according to their coordinate regulation by growth The mechanism of cell death in tumor cells is unclear. In the present arrest and DNA damage (13). The GADD family members include study, we investigated the pathways underlying apoptosis. Apo- GADD34,-45␣,-45,-45␥, and -153 (14, 15). These are stress- ptosis was seen in a tumor cell-specific manner in RKO cells but not response genes induced by both genotoxic and nongenotoxic in normal renal epithelial cells, despite inhibition of SIM2-s expres- stresses (16–18). GADD45␣ is the most extensively studied mem- sion in both of these cells by the antisense. -
Genetic Deletion Ofgadd45b, a Regulator of Active DNA
The Journal of Neuroscience, November 28, 2012 • 32(48):17059–17066 • 17059 Brief Communications Genetic Deletion of gadd45b, a Regulator of Active DNA Demethylation, Enhances Long-Term Memory and Synaptic Plasticity Faraz A. Sultan,1 Jing Wang,1 Jennifer Tront,2 Dan A. Liebermann,2 and J. David Sweatt1 1Department of Neurobiology and Evelyn F. McKnight Brain Institute, University of Alabama at Birmingham, Birmingham, Alabama 35294 and 2Fels Institute for Cancer Research and Molecular Biology, Temple University, Philadelphia, Pennsylvania 19140 Dynamic epigenetic mechanisms including histone and DNA modifications regulate animal behavior and memory. While numerous enzymes regulating these mechanisms have been linked to memory formation, the regulation of active DNA demethylation (i.e., cytosine-5 demethylation) has only recently been investigated. New discoveries aim toward the Growth arrest and DNA damage- inducible 45 (Gadd45) family, particularly Gadd45b, in activity-dependent demethylation in the adult CNS. This study found memory- associated expression of gadd45b in the hippocampus and characterized the behavioral phenotype of gadd45b Ϫ/Ϫ mice. Results indicate normal baseline behaviors and initial learning but enhanced persisting memory in mutants in tasks of motor performance, aversive conditioning and spatial navigation. Furthermore, we showed facilitation of hippocampal long-term potentiation in mutants. These results implicate Gadd45b as a learning-induced gene and a regulator of memory formation and are consistent with its potential role in active DNA demethylation in memory. Introduction along with the finding of activity-induced gadd45b in the hip- Alterations in neuronal gene expression play a necessary role pocampus led us to hypothesize that Gadd45b modulates mem- in memory consolidation (Miyashita et al., 2008). -
Advanced Sectioned Images of a Cadaver Head with Voxel Size Of
J Korean Med Sci. 2019 Sep 2;34(34):e218 https://doi.org/10.3346/jkms.2019.34.e218 eISSN 1598-6357·pISSN 1011-8934 Original Article Advanced Sectioned Images of a Cadaver Basic Medical Sciences Head with Voxel Size of 0.04 mm Beom Sun Chung ,1 Miran Han ,2 Donghwan Har ,3 and Jin Seo Park 4 1Department of Anatomy, Ajou University School of Medicine, Suwon, Korea 2Department of Radiology, Ajou University School of Medicine, Suwon, Korea 3College of ICT Engineering, Chung Ang University, Seoul, Korea 4Department of Anatomy, Dongguk University School of Medicine, Gyeongju, Korea Received: Jun 14, 2019 Accepted: Jul 22, 2019 ABSTRACT Address for Correspondence: Background: The sectioned images of a cadaver head made from the Visible Korean project Jin Seo Park, PhD have been used for research and educational purposes. However, the image resolution Department of Anatomy, Dongguk University is insufficient to observe detailed structures suitable for experts. In this study, advanced School of Medicine, 87 Dongdae-ro, Gyeongju sectioned images with higher resolution were produced for the identification of more 38067, Republic of Korea. E-mail: [email protected] detailed structures. Methods: The head of a donated female cadaver was scanned for 3 Tesla magnetic resonance © 2019 The Korean Academy of Medical images and diffusion tensor images (DTIs). After the head was frozen, the head was Sciences. sectioned serially at 0.04-mm intervals and photographed repeatedly using a digital camera. This is an Open Access article distributed Results: On the resulting 4,000 sectioned images (intervals and pixel size, 0.04 mm3; color under the terms of the Creative Commons Attribution Non-Commercial License (https:// depth, 48 bits color; a file size, 288 Mbytes), minute brain structures, which can be observed creativecommons.org/licenses/by-nc/4.0/) not on previous sectioned images but on microscopic slides, were observed. -
Gadd45a Regulates B-Catenin Distribution and Maintains Cell–Cell Adhesion/ Contact
Oncogene (2007) 26, 6396–6405 & 2007 Nature Publishing Group All rights reserved 0950-9232/07 $30.00 www.nature.com/onc ORIGINAL ARTICLE Gadd45a regulates b-catenin distribution and maintains cell–cell adhesion/ contact JJi1, R Liu1, T Tong, Y Song, S Jin, M Wu and Q Zhan State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China Gadd45a,a growth arrest and DNA-damage gene,plays 1999;Jin et al., 2000;Tong et al., 2005). Gadd45a important roles in the control of cell cycle checkpoints, also plays roles in negative regulation of cell malig- DNA repair and apoptosis. We show here that Gadd45a is nancy. Mouse embryonic fibroblasts (MEFs) derived involved in the control of cell contact inhibition and cell– from Gadd45a-null mice exhibited genomic instability, cell adhesion. Gadd45a can serve as an adapter to enhance single oncogene-mediated transformation, loss of nor- the interaction between b-catenin and Caveolin-1,and in mal cellular senescence, increased cellular proliferation, turn induces b-catenin translocation to cell membrane for centrosome amplification and reduced DNA repair maintaining cell–cell adhesion/contact inhibition. This is (Hollander et al., 1999;Smith et al., 2000). Gadd45a- coupled with reduction of b-catenin in cytoplasm and null mice have an increased frequency of tumorigenesis nucleus following Gadd45a induction,which is reflected by by ionizing radiation (IR), ultraviolet radiation (UVR) the downregulation of cyclin D1,one of the b-catenin and dimethylbenzanthracene (DMBA), although these targeted genes. Additionally,Gadd45a facilitates ultra- mice do not develop spontaneous tumors (Hollander violet radiation-induced degradation of cytoplasmic and et al., 1999, 2001;Hildesheim et al., 2002). -
State of Connecticut
REQUEST FOR PROPOSAL UCHC RFP-01 Form Rev. 1/12 ACKNOWLEDGMENT: RECEIPT OF RFP DOCUMENTS Buyer STATE OF CONNECTICUT UNIVERSITY OF CONNECTICUT HEALTH CENTER Telephone Number Procurement Operations & Contracts 263 Farmington Avenue, MC4036 E-mail Address Farmington, CT 06032-4036 Fax Number RFP NUMBER: PROPOSAL DUE DATE: PROPOSAL DUE TIME: RFP SURETY: EST RFP TITLE: NOTE: Please complete and return this acknowledgement as soon as possible to the Buyer’s email address or fax number indicated above. This document is crucial for proposal follow-up. Please check one of the following boxes: Submitting a proposal NOT submitting a proposal Print or type the following information: Firm/Corporation Name: Street Address: City, State, Zip Code: Contact Name/Title: Phone Number: Fax Number: E-mail Address: Page 1 of 1 REQUEST FOR PROPOSAL UCHC RFP-02 Form Rev. 8/12 PROPOSER’S CHECKLIST Buyer STATE OF CONNECTICUT UNIVERSITY OF CONNECTICUT HEALTH CENTER Telephone Number Procurement Operations & Contracts 263 Farmington Avenue, MC4036 E-mail Address Farmington, CT 06032-4036 Fax Number RFP NUMBER: PROPOSAL DUE DATE: PROPOSAL DUE TIME: RFP SURETY: EST RFP TITLE: IT IS SUGGESTED THAT YOU REVIEW AND CHECK OFF EACH ACTION ITEM AS YOU COMPLETE IT CHECK 1. Form UCHC RFP-01 (Acknowledgement: Receipt of RFP Documents) must be completed and returned at least 48 hours before the proposal due date and time listed on page 1 of this form. 2. If required, the amount of proposal surety has been checked and the surety has been included in the upper right hand corner of all forms except form UCHC RFP-05 (CHRO & SEEC Compliance). -
DNA Excision Repair Proteins and Gadd45 As Molecular Players for Active DNA Demethylation
Cell Cycle ISSN: 1538-4101 (Print) 1551-4005 (Online) Journal homepage: http://www.tandfonline.com/loi/kccy20 DNA excision repair proteins and Gadd45 as molecular players for active DNA demethylation Dengke K. Ma, Junjie U. Guo, Guo-li Ming & Hongjun Song To cite this article: Dengke K. Ma, Junjie U. Guo, Guo-li Ming & Hongjun Song (2009) DNA excision repair proteins and Gadd45 as molecular players for active DNA demethylation, Cell Cycle, 8:10, 1526-1531, DOI: 10.4161/cc.8.10.8500 To link to this article: http://dx.doi.org/10.4161/cc.8.10.8500 Published online: 15 May 2009. Submit your article to this journal Article views: 135 View related articles Citing articles: 92 View citing articles Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=kccy20 Download by: [University of Pennsylvania] Date: 27 April 2017, At: 12:48 [Cell Cycle 8:10, 1526-1531; 15 May 2009]; ©2009 Landes Bioscience Perspective DNA excision repair proteins and Gadd45 as molecular players for active DNA demethylation Dengke K. Ma,1,2,* Junjie U. Guo,1,3 Guo-li Ming1-3 and Hongjun Song1-3 1Institute for Cell Engineering; 2Department of Neurology; and 3The Solomon Snyder Department of Neuroscience; Johns Hopkins University School of Medicine; Baltimore, MD USA Abbreviations: DNMT, DNA methyltransferases; PGCs, primordial germ cells; MBD, methyl-CpG binding protein; NER, nucleotide excision repair; BER, base excision repair; AP, apurinic/apyrimidinic; SAM, S-adenosyl methionine Key words: DNA demethylation, Gadd45, Gadd45a, Gadd45b, Gadd45g, 5-methylcytosine, deaminase, glycosylase, base excision repair, nucleotide excision repair DNA cytosine methylation represents an intrinsic modifica- silencing of gene activity or parasitic genetic elements (Fig. -
The Virtual Hip: an Anatomically Accurate Finite Element Model Based on the Visible Human Dataset
University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School 10-4-2010 The Virtual Hip: An Anatomically Accurate Finite Element Model Based on the Visible Human Dataset Jonathan M. Ford University of South Florida Follow this and additional works at: https://scholarcommons.usf.edu/etd Part of the American Studies Commons, Biomedical Engineering and Bioengineering Commons, and the Chemical Engineering Commons Scholar Commons Citation Ford, Jonathan M., "The Virtual Hip: An Anatomically Accurate Finite Element Model Based on the Visible Human Dataset" (2010). Graduate Theses and Dissertations. https://scholarcommons.usf.edu/etd/3451 This Thesis is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. The Virtual Hip: An Anatomically Accurate Finite Element Model Based on the Visible Human Dataset by Jonathan M. Ford A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Biomedical Engineering Department of Chemical and Biomedical Engineering College of Engineering University of South Florida Co-Major Professor: Don Hilbelink, Ph.D. Co-Major Professor: Les Piegl, Ph.D. William Lee III, Ph.D., P.E. Karl Muffly, Ph.D. Date of Approval: October 4, 2010 Keywords: Quantitative Anatomy, Gluteus Minimus, COMSOL, Model Error Copyright © 2010, Jonathan M. Ford DEDICATION I would like to dedicate this thesis to “B” and Vegas. They pushed me to reach for the stars and when I lost my direction they were the ones to point me the way. -
High Performance Computing and Communications: Toward a National Information Infrastructure
DOCUMENT RESUME ED 368 330 IR 016 567 TITLE High Performance Computing and Communications: Toward a National Information Infrastructure. INSTITUTION Federal Coordinating Council for Science, Engineering and Technology, Washington, DC. PUB DATE 94 NOTE 190p.; Illustrations and photographs may not copy adequately. PUB TYPE Reports Evaluative/Feasibility (142) EDRS PRICE MF01/PC08 Plus Postage. DESCRIPTORS Computer Centers; Computer Networks; *Educational Technology; Government Role; Information Networks; Program Evaluation; Research and Development; *Technological Advancement; *Telecommunications; Training IDENTIFIERS *High Performance Computing; High Performance Computing Act 1991; *National Information Infrastructure; National Research and Education Network ABSTRACT This report describes the High Performance Computing and Communications (HPCC) initiative of the Federal Coordinating Council for Science, Engineering, and Technology. This program is supportive of and coordinated with the National Information Infrastructure Initiative. Now halfway through its 5-year effort, the HPCC program counts among its achievements more than a dozen high-performance computing centers in operation nationwide. Traffic on federally funded networks and the number of local and regional networks connected to these centers continues to double each year. Teams of researchers have made substantial progress in adapting software for use on high-performance computer systems; and the base of researchers, educators, and students trained in HPCC technologies has grown substantially. The five HPCC program components in operation at present are:(1) scalable computing systems ranging from affordable workstations to large-scale high-performance systems; (2) the National Research and Education Network;(3) the Advanced Software Technology and Algorithms program; (4) the Information Infrastructure Technology and Applications program; and (5) the Bas.c Research and Human Resources program. -
Spatiotemporal Expression Pattern of Gadd45g Signaling Pathway
Metab y & o g lic lo S o y n n i r d Labrie et al. Endocrinol Metabol Syndrome 2011, S:4 c r o o m d n e DOI: 10.4172/2161-1017.S4-005 E Endocrinology & Metabolic Syndrome ISSN: 2161-1017 Research Article Open Access Spatiotemporal Expression Pattern of Gadd45g Signaling Pathway Components in the Mouse Uterus Following Hormonal Steroid Treatments Yvan Labrie, Karine Plourde, Johanne Ouellet, Geneviève Ouellette, Céline Martel, Fernand Labrie, Georges Pelletier and Francine Durocher* Oncology and Molecular Endocrinology Research Centre, CHUQ Research Centre, CHUL, Department of Molecular Medicine, Laval University, Québec, G1V 4G2, Canada Abstract The uterus is a hormone-dependent organ under the control of estrogens, progestins and androgens. The actions of estradiol (E2) and progesterone on uterine physiology are well documented, while active androgens such as testosterone and dihydrotestosterone (DHT) are now also recognized to exert an important role to appropriately maintain the cyclical changes of the endometrium. Based on our previous observations that DHT modulates GADD45g and the expression of specific cell cycle genes, the aim of this study was to identify the specific uterine cell types in which these cell cycle genes are modulated by hormonal steroids. Using in situ hybridization and quantitative RT-PCR (QRT-PCR), the localization and measurement of mRNA expression of key cell cycle genes responding to E2 and DHT were performed in the uterus of ovariectomized mice. Interestingly, in situ hybridization experiments demonstrate that Gadd45g mRNA is detected early and strongly in stromal cells only, indicating that the early cell cycle arrest induced by DHT and E2 in the mouse uterus occurs in this compartment.