Inactivation Efficiency to Bacillus Subtilis and Escherichia Coli Bacterial Aerosols of Spraying Neutral Electrolyzed Water
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Journal of the Air & Waste Management Association ISSN: 1096-2247 (Print) 2162-2906 (Online) Journal homepage: http://www.tandfonline.com/loi/uawm20 Inactivation efficiency to Bacillus subtilis and Escherichia coli bacterial aerosols of spraying neutral electrolyzed water Chi-Yu Chuang , Shinhao Yang , Ming-Yih Chang , Hsiao-Chien Huang , Chin- Hsiang Luo , Po-Chen Hung & Wei Fang To cite this article: Chi-Yu Chuang , Shinhao Yang , Ming-Yih Chang , Hsiao-Chien Huang , Chin-Hsiang Luo , Po-Chen Hung & Wei Fang (2013) Inactivation efficiency to Bacillussubtilis and Escherichiacoli bacterial aerosols of spraying neutral electrolyzed water, Journal of the Air & Waste Management Association, 63:12, 1447-1456, DOI: 10.1080/10962247.2013.827604 To link to this article: https://doi.org/10.1080/10962247.2013.827604 Accepted author version posted online: 06 Aug 2013. Published online: 06 Aug 2013. Submit your article to this journal Article views: 598 Citing articles: 4 View citing articles Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=uawm20 TECHNICAL PAPER Inactivation efficiency to Bacillus subtilis and Escherichia coli bacterial aerosols of spraying neutral electrolyzed water ⁄ Chi-Yu Chuang,1 Shinhao Yang,2, Ming-Yih Chang,3 Hsiao-Chien Huang,2 Chin-Hsiang Luo,4 Po-Chen Hung,5 and Wei Fang1 1Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei, Taiwan, Republic of China 2Center for General Education, Toko University, Taiwan, Republic of China 3Department of Biomechatronics Engineering, National Ilan University, Taiwan, Republic of China 4Department of Safety, Health and Environmental Engineering, Hungkuang University, Taiwan, Republic of China 5Institute of Occupational Safety and Health, Council of Labor Affairs, Taiwan, Republic of China ⁄ Please address correspondence to: Shinhao Yang, 51 University Rd., Sec. 2, Pu-tzu City, Chia Yi County 613, Taiwan, Republic of China; e-mail: [email protected] The main objective of this study is to apply neutral electrolyzed water (NEW) spraying to inactivate bioaerosols. We evaluated the inactivation efficiency of NEWapplied to inactivate two airborne bacterial Escherichia coli and Bacillus subtilis aerosols inside an environmental-controlled chamber in the study. Generated with electrolyzing 6.15 M sodium chloride brine, the NEW with free available chlorine (FAC) concentration 50, 100, and 200 ppm was pumped with an air pressure of 70 kg/cm2 through nozzle into the chamber to inactive E. coli and B. subtilis aerosols precontaminated air (initial counts of 3 Â 104 colony-forming units [CFU]/m3). Bacterial aerosols were collected and cultured from chamber before and after NEW spray. The air exchange rate (ACH, hrÀ1) of the chamber was set to simulate fresh air ventilating dilution of indoor environment. First-order concentration decaying coefficients (Ka, minÀ1) of both bacterial aerosols were measured as an index of NEW inactivation efficiency. The result shows that higher FAC concentration of NEW spray caused better inactivation efficiency. The Ka values under ACH 1.0 hrÀ1 were 0.537 and 0.598 for E. coli of FAC 50 and 100 ppm spraying, respectively. The Ka values of FAC 100 ppm and 200 ppm spraying for B. subtilis were 0.063 and 0.085 under ACH 1.0 hrÀ1, respectively. The results indicated that NEW spray is likely to be effective in inactivation of bacterial airborne contamination. Moreover, it is observed in the study that the increase of ventilation rate and the use of a larger orifice-size nozzle may facilitate the inactivation efficiency. Implications: Bacterial aerosols have been implicated in deterioration of air quality and occupational health. Effective, safe, and economic control technology is highly demanded, especially for agricultural and food industries. In the study, NEW mist spraying performed effectively in controlling E. coli and B. subtilis modeling bioaerosols contamination. The NEW revealed its potential as an alternative airborne disinfectant worth being discovered for improving the environmental quality in the future. Introduction were found in a swine confinement building investigation in Canada (Cormier et al., 1998; Cormier et al., 2000). There was Biological contamination in agricultural and 2.8 Â 104 CFU/m3 of respirable airborne microorganisms recov- food-processing facilities ered when Predicala et al. studied the bioaerosols concentration in the swine finishing barns (Predicala et al., 2002). An on-site Nowadays, indoor airborne biological contamination has survey in swine houses in Korea found 4 log CFU/m3 for total raised public health concerns worldwide (Douwes et al., 2003; airborne bacteria where air quality management is demanded Schenker et al., 1998). Exposure to high level of airborne bacter- (Kim et al., 2007). High density of bacterial aerosols and related ial aerosols in agricultural and food-processing facilities such as endotoxin exposure may lead to adverse health effect of poultry greenhouses and swine and poultry housing may cause adverse workers. The workers were reported to be high-prevalence health effects. This has become an important issue that led to groups for work-related eye, respiratory, and skin symptoms in intensive investigation in recent years (Lacey and Dutkiewicz, previous studies (Lacey and Dutkiewicz, 1994; Radon et al., 1994; Heederik and Sigsgaard, 2005; Mackiewicz et al., 1999). 2002; Heederik and Sigsgaard, 2005; Heederik et al., 2007). In High airborne bacterial aerosols concentrations (up to 105 greenhouse facilities, occupational asthma and rhinitis due to the colony-forming units [CFU]/m3) for nasal breathing exposure exposure to microorganisms and endotoxin during growth 1447 Journal of the Air & Waste Management Association, 63(12):1447–1456, 2013. Copyright © 2013 A&WMA. ISSN: 1096-2247 print DOI: 10.1080/10962247.2013.827604 1448 Chuang et al. / Journal of the Air & Waste Management Association 63 (2013) 1447–1456 seasons have been reported in various investigations (Adhikari NEW has been reported to pose creditable antimicrobial et al., 2010; Radon et al., 2002). High concentrations of bioaer- reactions against a variety of microorganisms in agricultural osols and endotoxin exposure of greenhouse workers harvesting and food industries. The performance of bactericidal efficiency cucumbers and tomatoes were detected in Denmark (Madsen of diluted NEW against E. coli O157:H7, Erwinia carotova, et al., 2009). The exposure to high-concentration bioaerosols can Salmonella enteritidis, and Listeria monocytogenes is at 1–2 also be identified in food facilities. More than 105 CFU/m3 of log units (Abadias et al., 2008). NEW was also revealed to be bacterial aerosols concentration in a noodle factory was effective sanitizer for reducing the presence of E. coli, observed in central Taiwan (Tsai and Liu, 2009). An investiga- L. monocytogenes, P. aeruginosa, and Staphylococcus aureus tion of a high-throughput chicken slaughter facility showed high on stainless-steel and glass surfaces with 6 log CFU/50 cm2 counts of E. coli, Bacillus cereus, Staphylococcus aureus, reduction (Deza, Araujo, and Garrido, 2005). Methicillin- Pseudomonas aeruginosa, and Salmonella spp. in the airborne resistant S. aureus (MRSA) and Acinetobacter baumannii were microbial levels; controlling measures are recommended before inoculated on the surface of ceramic tiles and was reduced by processing materials to prevent the spread of microorganisms 106.8-fold with electrolyzed water fogging treatment (Clark et al., downstream (Lues et al. 2007). 2006). Escherichia coli O157:H7 food-borne pathogenic strains were spot-inoculated on lettuce leaves and were significantly Inactivating mechanism of electrolyzed water on reduced by NEW (Pangloli and Hung, 2011). bacteria According to the investigation conducted by Monnin (Monnin, Lee, and Pascall, 2012), E. coli K12 and L. innocua Facing the air quality problem caused by airborne microor- were significantly sanitized from cutting boards by 4 log CFU/ ganisms in the agricultural and food facilities, researchers have 100 m2 with NEW. In field application, as an alternative for shown interest in the use of chemical technologies to reduce chemicals traditionally used for bactericidal purposes, electro- airborne bacteria without causing harmful effects to workers, lyzed water is gaining popularity due to its strong bactericidal food materials, and animals. Electrolyzed water is generated by effects when used on food and equipment surfaces and its electrolysis of saline brine in a cell within anodic and cathodic advantages such as safety and nonirritating response of mucous electrodes with or without ion-selective permeating membrane. membranes and skins. In addition, the electrolyzed water also The electrolyzed water contains high oxidation–reduction poten- has the advantages of being less toxic and causing less adverse tial (ORP) and free available chlorine (FAC) compounds (hypo- environmental impact than other chemical disinfectants (Graça chlorous acid HOCl, chlorine gas Cl2, and hypochlorite ion et al., 2011; Arevalos-Sánchez et al., 2012; McCarthy and OClÀ), resulting in strong antimicrobial activity (Huang et al., Burkhardt Iii, 2012; Guentzel et al., 2008; Zheng et al., 2012). 2008). Studies on disinfecting mechanisms found that electro- Despite the widely proven effectiveness bacterial contamina- lyzed water performs dehydrogenase activities on E. coli and tion on food products, food